CN103891774A - Seed disinfectant - Google Patents
Seed disinfectant Download PDFInfo
- Publication number
- CN103891774A CN103891774A CN201210585570.2A CN201210585570A CN103891774A CN 103891774 A CN103891774 A CN 103891774A CN 201210585570 A CN201210585570 A CN 201210585570A CN 103891774 A CN103891774 A CN 103891774A
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- CN
- China
- Prior art keywords
- weight
- percetage
- seed disinfectant
- shitosan
- distilled water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The invention relates to a seed disinfectant which is characterized by comprising the following ingredients in percentage by weight: 1-2 percent of chitosan, 0.5-2 percent of KMnO4, 15 percent of ethanol and the balance being distilled water. According to the seed disinfectant disclosed by the invention, a synergistic effect among the ingredients is achieved, the disinfection effect is obvious, the application range is wide, the seed disinfectant is suitable for disinfection of various plant seeds, and a preparation method is simple.
Description
Technical field
The present invention relates to a kind of plant seed disinfectant.
Background technology
In Plant Tissue Breeding and gene engineering research, to explant disinfection, be very important previous work, seed disinfection is even more important.In the research of molecular biotechnology, aseptic seedling is desirable test starting material, often need to cultivate aseptic seedling.In group training, using aseptic seedling as explant, can obtain callus and regeneration plant, further carry out transgeneic procedure; In high-quality RNA extracts, also to use aseptic seedling.
The sterilize disinfectant that uses of plant seed has multiplely, common are hypochlorite, hydrogen peroxide, bromine water, mercury chloride, alcohol etc., offsets the research of chemical agent decontamination effect, and forefathers have done a large amount of work.
Shitosan (chitosan) is that the chitin (chitin) extensively being existed by natural world obtains through deacetylation, the premium properties such as the biological functionality of this natural polymer and compatibility, blood compatibility, safety, microbic resolvability, by all trades and professions extensive concern, have been obtained major progress in the application study of the numerous areas such as medicine, food, chemical industry, cosmetics, water treatment, METAL EXTRACTION and recovery, biochemistry and biomedical engineering.Shitosan is easy to dissolve in weak acid solvent, contains amino (NH2+) in the solution after dissolving, and these amino are by carrying out anti-bacteria in conjunction with negatron.The Antibacterial activity of shitosan, makes it have a wide range of applications in fields such as medicine, weaving and food.
Summary of the invention
Technical problem to be solved of the present invention is that a kind of environmental protection, efficient biological seed disinfectant are provided.
For solveing the technical problem, the technical solution used in the present invention is:
A kind of seed disinfectant, contains the shitosan that percetage by weight is 1%-2%, the KMnO that percetage by weight is 0.5%-2%
4, the ethanol that percetage by weight is 15% and the distilled water of surplus.
Optimize, a kind of seed disinfectant, contains percetage by weight and is 1% shitosan, the KMnO that percetage by weight is 1%
4, the ethanol that percetage by weight is 15% and the distilled water of surplus.
Seed disinfectant of the present invention, its each composition synergy, Disinfection Effect is remarkable, has wide range of applications, and is applicable to the sterilization of various plant seeds, and compound method is simple.
Embodiment
Embodiment 1
A kind of seed disinfectant, contains percetage by weight and is 1% shitosan, the KMnO that percetage by weight is 1%
4, the ethanol that percetage by weight is 15% and the distilled water of surplus.Compound method is: get 1g shitosan, 1gKMnO
4with 15 grams of ethanol solutions, be dissolved in 83 grams of distilled water.
Embodiment 2
A kind of seed disinfectant, contains percetage by weight and is 2% shitosan, the KMnO that percetage by weight is 0.5%
4, the ethanol that percetage by weight is 15% and the distilled water of surplus.Compound method is: get 2g shitosan, 0.5gKMnO
4with 15 grams of ethanol solutions, be dissolved in 82.5 grams of distilled water.
Embodiment 3
A kind of seed disinfectant, contains percetage by weight and is 1.5% shitosan, the KMnO that percetage by weight is 2%
4, the ethanol that percetage by weight is 15% and the distilled water of surplus. and compound method is: get 1.5g shitosan, 2gKMnO
4with 15 grams of ethanol solutions, be dissolved in 81.5 grams of distilled water.
Claims (2)
1. a seed disinfectant, is characterized in that: contain the shitosan that percetage by weight is 1%-2%, the KMnO that percetage by weight is 0.5%-2%
4, the ethanol that percetage by weight is 15% and the distilled water of surplus.
2. disinfectant according to claim 1, is characterized in that: contain percetage by weight and be 1% shitosan, the KMnO that percetage by weight is 1%
4, the ethanol that percetage by weight is 15% and the distilled water of surplus.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210585570.2A CN103891774A (en) | 2012-12-30 | 2012-12-30 | Seed disinfectant |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210585570.2A CN103891774A (en) | 2012-12-30 | 2012-12-30 | Seed disinfectant |
Publications (1)
Publication Number | Publication Date |
---|---|
CN103891774A true CN103891774A (en) | 2014-07-02 |
Family
ID=50983608
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201210585570.2A Pending CN103891774A (en) | 2012-12-30 | 2012-12-30 | Seed disinfectant |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103891774A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0878129A1 (en) * | 1997-05-15 | 1998-11-18 | Iskra Industry Co., Ltd. | Composition comprising chitosan for enhancing resistance to plant diseases |
CN1221554A (en) * | 1997-12-30 | 1999-07-07 | 中国科学院上海植物生理研究所 | Coated seeds and its prepn. method |
-
2012
- 2012-12-30 CN CN201210585570.2A patent/CN103891774A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0878129A1 (en) * | 1997-05-15 | 1998-11-18 | Iskra Industry Co., Ltd. | Composition comprising chitosan for enhancing resistance to plant diseases |
CN1221554A (en) * | 1997-12-30 | 1999-07-07 | 中国科学院上海植物生理研究所 | Coated seeds and its prepn. method |
Non-Patent Citations (4)
Title |
---|
宋淑淑 等: "壳聚糖对棉花种子萌发及相关酶活性的影响", 《种子》 * |
李桂珍 等: "种子处理对玉米矮花叶病的防治效果", 《植保技术与推广》 * |
杨文平 等: "天达-2116拌种对冬小麦新麦18种子萌发和幼苗生长特性的影响", 《广东农业科学》 * |
王云 等: "壳聚糖对镉胁迫条件下小麦生长及生理的影响", 《生态学杂志》 * |
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PB01 | Publication | ||
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SE01 | Entry into force of request for substantive examination | ||
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140702 |