CN105062957B - 血管内皮细胞祖细胞的培养方法 - Google Patents
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CN105602888A (zh) * | 2016-01-12 | 2016-05-25 | 温州医科大学附属第一医院 | 一种内皮祖细胞快速扩增体系 |
CN107326006B (zh) * | 2017-07-21 | 2018-05-08 | 臻和(北京)科技有限公司 | 一种人胚胎干细胞分化为内皮细胞专用的培养基及其方法 |
CN108048390B (zh) * | 2017-12-21 | 2021-04-27 | 清华大学 | 一种制备血管内皮细胞的方法及其专用试剂盒 |
CN108315293A (zh) * | 2018-01-31 | 2018-07-24 | 溯源生命科技股份有限公司 | 一种血管内皮祖细胞的制备方法 |
CN110713984B (zh) * | 2018-11-27 | 2024-01-23 | 四川大学 | 诱导人间充质干细胞生成功能内皮细胞的方法 |
CN111440760B (zh) * | 2020-03-09 | 2022-09-23 | 浙江大学 | 一种人多能干细胞高效分化得到内皮祖细胞的方法 |
CN113046318B (zh) * | 2021-04-13 | 2023-04-18 | 中国科学院深圳先进技术研究院 | 一种诱导多能干细胞向造血前体细胞分化的培养基以及方法 |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102732483A (zh) * | 2011-03-31 | 2012-10-17 | 北京大学 | 造血祖细胞的制备方法及其专用培养基 |
CN103834613A (zh) * | 2012-11-27 | 2014-06-04 | 中国科学院上海生命科学研究院 | 制备多能心血管前体细胞及维持其心血管分化能力的方法 |
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CN102732483A (zh) * | 2011-03-31 | 2012-10-17 | 北京大学 | 造血祖细胞的制备方法及其专用培养基 |
CN103834613A (zh) * | 2012-11-27 | 2014-06-04 | 中国科学院上海生命科学研究院 | 制备多能心血管前体细胞及维持其心血管分化能力的方法 |
Non-Patent Citations (1)
Title |
---|
调节ALK5受体活化水平优化间充质干细胞的内皮分化及组织工程血管的应用;艾文佳等;《中山大学学报》;20130331;第34卷(第2期);207-214 * |
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