CN105028275B - A kind of aliphatic acid compensation method of hippocampus seedling - Google Patents
A kind of aliphatic acid compensation method of hippocampus seedling Download PDFInfo
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- CN105028275B CN105028275B CN201510399598.0A CN201510399598A CN105028275B CN 105028275 B CN105028275 B CN 105028275B CN 201510399598 A CN201510399598 A CN 201510399598A CN 105028275 B CN105028275 B CN 105028275B
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- 210000001320 hippocampus Anatomy 0.000 title claims abstract description 127
- 238000000034 method Methods 0.000 title claims abstract description 46
- 239000002253 acid Substances 0.000 title claims abstract description 15
- 125000001931 aliphatic group Chemical group 0.000 title claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 18
- 241000143060 Americamysis bahia Species 0.000 claims abstract description 13
- 241000239250 Copepoda Species 0.000 claims abstract description 13
- 241000238557 Decapoda Species 0.000 claims abstract description 13
- 241001247197 Cephalocarida Species 0.000 claims abstract description 10
- 241000159660 Nannochloropsis oculata Species 0.000 claims abstract description 10
- 239000011259 mixed solution Substances 0.000 claims abstract description 8
- 238000009395 breeding Methods 0.000 claims abstract description 7
- 230000001488 breeding effect Effects 0.000 claims abstract description 7
- 238000007654 immersion Methods 0.000 claims description 6
- 230000036541 health Effects 0.000 claims description 4
- 241000238582 Artemia Species 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 13
- 230000000052 comparative effect Effects 0.000 description 30
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 12
- 235000016709 nutrition Nutrition 0.000 description 8
- 230000035764 nutrition Effects 0.000 description 7
- 230000003014 reinforcing effect Effects 0.000 description 7
- 101000831205 Danio rerio Dynein axonemal assembly factor 11 Proteins 0.000 description 6
- 102100024282 Dynein axonemal assembly factor 11 Human genes 0.000 description 6
- 241001559542 Hippocampus hippocampus Species 0.000 description 6
- 101000831210 Homo sapiens Dynein axonemal assembly factor 11 Proteins 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 5
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 235000013305 food Nutrition 0.000 description 3
- 230000001228 trophic effect Effects 0.000 description 3
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 150000004965 peroxy acids Chemical class 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241001559546 Hippocampus erectus Species 0.000 description 1
- 241000595940 Notostraca Species 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000002079 cooperative effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a kind of aliphatic acid compensation method of hippocampus seedling, methods described step are as follows：S1. hippocampus parent is screened；S2. frost oppossum shrimp and 15 age in days shrimps are soaked respectively using bait hardening agent, feed parent daily 3 times；S3. the hippocampus parent fed in S2 is mated, after breeding out hippocampus seedling, is placed in other young pond seedling and cultivates；S4. hippocampus seedling bait is soaked using the mixed solution for intending Nannochloropsis oculata and bait hardening agent, and fed to the hippocampus seedling just hatched in S3, fed daily 6 ~ 8 times；The hippocampus seedling bait is the artemia and Copepods just hatched.The aliphatic acid and fat content in hippocampus seedling body can be significantly improved according to method provided by the invention, and survival rate and growth rate can be significantly improved.
Description
Technical field
The invention belongs to hippocampus method for culturing seedlings technical field, more specifically, more particularly to a kind of aliphatic acid of hippocampus seedling
Compensation method.
Background technology
In recent years, sea horse cultivation has become the new industry of China's mariculture industry, and there has been family more than 300 country at present
Sea horse cultivation enterprise, strain line hippocampus（Hippocampus erectus）It is main cultivation species.In the world, sea horse cultivation
Two big bottlenecks are the low survival rate of hippocampus seedling and the low reproductive efficiency of adult hippocampus respectively, wherein, the bait battalion of hippocampus seedling
Foster deficiency is one of principal element for causing the low survival rate of seedling, and hippocampus Seedling Stage needs substantial amounts of higher unsaturated fatty acid.
Accordingly, substantial amounts of exploration has been done for different aspects such as the bait composition of hippocampus seedling, trophic structure, feeding methods in the world
How report, specifically optimize above-mentioned influence factor, and the not research of related conclusions, causes each method difference at present
Between cultivation result is had a great influence, another also existing problem is due to the breeding characteristic of hippocampus seedling at present, its survival rate
It is difficult to always more than 90%.Therefore, the present invention seeks to obtain a kind of aliphatic acid compensation method of hippocampus, it is possible to increase it is in vivo not
Saturated fatty acid content, preferable culture efficiency is obtained, lift its survival rate.It is currently based on and parent hippocampus and seedling bait is entered
Row fortification, and then the report of the aliphatic acid supplement of compensating property of hippocampus seedling is rarely reported.
The content of the invention
It is an object of the invention to the deficiency according to existing hippocampus seedling technology, there is provided a kind of aliphatic acid of hippocampus seedling
Compensation method, the survival rate and growth rate of hippocampus seedling can be significantly improved according to method provided by the invention, is supported for hippocampus
Grow, especially strain line sea horse cultivation, there is preferable application prospect.
The above-mentioned purpose of the present invention is achieved through the following technical solutions：
The invention provides a kind of aliphatic acid compensation method of hippocampus seedling, methods described step are as follows：
S1. hippocampus parent is screened；
S2. frost oppossum shrimp and 15 age in days shrimps are soaked respectively using bait hardening agent, feed parent daily 3 times；
S3. the hippocampus parent fed in S2 is mated, after breeding out hippocampus seedling, is placed in other young pond seedling and supports
Grow；
S4. hippocampus seedling bait is soaked using the mixed solution for intending Nannochloropsis oculata and bait hardening agent, and fed in S3
The hippocampus seedling just hatched, feed daily 6 ~ 8 times；
The hippocampus seedling bait is the artemia and Copepods just hatched.
The present invention breeds seedling progress fortification using in hippocampus parent and its, and selects suitable bait to be raised
Support, establish a set of compensation method for being applied to improve hippocampus body fat and content of fatty acid.
Preferably, in S2 during immersion frost oppossum shrimp, it is 8 ~ 15ppm that bait, which strengthens agent concentration,；Soak time is 1 ~ 1.5h.
Preferably, when 15 age in days shrimps are soaked in S2, it is 5 ~ 10ppm that bait, which strengthens agent concentration,；Soak time is 4 ~ 7h.
Wherein, the present invention is to be replaced using between the frost oppossum shrimp of fortification and the shrimps of 15 ages in days in hippocampus parent's stage
Feeding pattern, specially 8：00、12：00 point feeds frost bait, and 17：00 point feeds live body shrimps.Mixing is replaced by
Pattern is fed, and can fully meet hippocampus parent's Stage Nutrition demand, improves unrighted acid and fat content in parent's body,
Offer nutritional support is bred for parent.
Preferably, in the mixed solution for intending Nannochloropsis oculata and bait hardening agent described in S4, intending Nannochloropsis oculata concentration is
1450 ~ 3600mg/L, the concentration of bait hardening agent is 5 ~ 10ppm.
The present invention intends Nannochloropsis oculata and commercially available higher unsaturated fatty acid is strengthened by using specific nutrition fortifier
The combination of agent, it can be that hippocampus parent breeds offer nutrition assurance, lift its internal unsaturated fatty acid content, and then lift it
Survival rate and growth rate.
Heretofore described bait hardening agent is Super Selco.
Preferably, the time of the immersion of mixed solution described in S4 hippocampus seedling bait is 5 ~ 8h.
Preferably, artemia described in S4 and the mixed proportion of Copepods are 2~4：6~8.
Preferably, artemia described in S4 and the mixed proportion of Copepods are 4：6.
Preferably, hippocampus parent is screened in S1 to select 4 ~ 5 monthly ages health, body surface is mellow and full, and ingest active hippocampus.
As described in background, in hippocampus seedling breeding process, its bait composition, corresponding food nutrition structure, feeding method
Cultivation will be had an impact, the present invention carries out reinforcing raising for parent's stage and hippocampus plantlet stage, with reference to optimization bait
Composition, play the reinforcing effect of nutrition fortifier simultaneously, obtain the rational composition supply raising hippocampus parent of trophic structure and
It breeds seedling, produces cooperative effect, and hippocampus seedling body fat and unsaturated fatty acids content are significantly improved, and enter one
Step ground, promotes its survival rate and growth rate.
Compared with prior art, the present invention has advantages below and beneficial effect：
The present invention improves the intrinsic PUFA contents of hippocampus parent by bait intensifying method, so as to ensure that hippocampus parent's is strong
Health is bred, and improves the PUFA of offspring seedling content indirectly；Secondly, based on to the seedling bait such as artemia, Copepods
Strengthen, directly improve the PUFA contents in hippocampus seedling body, and then improve the survival rate and growth rate of hippocampus.In addition, for parent
The food species and feed mode of this and plantlet stage optimize, and obtain preferable culture efficiency, this application technology it is excellent
Point is easy to operate, and controllability is strong, is adapted to large-scale commercialization sea horse cultivation.The hippocampus children of content of fatty acid is improved through the present invention
Seedling, survival rate 93.5%, growth rate improves 13.8%, possesses great application prospect.
Embodiment
Further illustrate this technology below in conjunction with specific embodiment, but embodiment this technology is not done it is any type of
Limit.Unless stated otherwise, the reagent of the invention used, method and apparatus is the art conventional reagent, methods and apparatus.
Specific growth rate（SGR）=100 ×（Ln final weight-ln original weights）/ experimental period
Embodiment 1
Present case is located at the Chinese Academy of Sciences South Sea base of sea horse cultivation in Shenzhen in March, 2014 to June, enforcement place.
The screening of 1 hippocampus parent
Experiment is 4-5 monthly age health strain line hippocampus with hippocampus（F3 generations）.Before experiment starts, by 1.2：1 ratio is from outdoor
Female, male hippocampus is selected in cement pit and amounts to 220, specific targets are as follows：（1）Male hippocampus：Select body length and be more than 12cm, body surface circle
Profit, vigor is good, ingests active；（2）Female hippocampus：To select body length and be more than 10cm, body surface is mellow and full, ingests active, belly both sides protuberance,
The wide 1.5-2 of a width of regular of belly or so body is accompanied, the slightly aobvious evagination of cloacal aperture.
The aquaculture management of 2 hippocampus parents
220 parents of this Setup Experiments（Female, male hippocampus=1.2：1）, every group has 10 each repetitions, each to repeat 22（Female,
Male hippocampus=1.2：1）.Hippocampus parent is placed on blue plastic barrel（The cm of base diameter 80,80 cm of height）Middle nursing, utilizes plastics
Pasture and water are as attachment, relevant environmental parameter：23-28 DEG C of water temperature, maximum illumination 2000-3000 lux, pH value 7.8-8.1,
Salinity 30-35, ammonia nitrogen concentration are not higher than 0.01mg/L.Continue to parent population fertility and breeding cycle uniformity carry out check and
It is determined that to ensure that hippocampus normally breeds.
3 bait are strengthened with feeding
The bait of hippocampus parent is fresh frost oppossum shrimp and 15 age in days shrimps, 3 bait is fed daily, 8：00、
12：00 point feeds frost bait, and 17：00 point feeds live body shrimps.Before being fed, with 8-15 ppm advanced insatiable hunger
And aliphatic acid（PUFA, SUPER SELCO, www.brineshrimpdirect.com）Immersion frost oppossum shrimp 1-1.5 hours,
Strengthened for 15 age in days shrimps 4-7 hours with 5-10 ppm PUFA.After feeding 30-40 days, the fat content in male and female hippocampus
The 1.2 ± 0.2% of hippocampus dry weight are accounted for, and the PUFA contents in male and female hippocampus account for the 33.7 ± 2.9% of total fat in vivo.
The reinforcing of 4 hippocampus seedling bait
The hippocampus parent that hippocampus seedling is derived from above-mentioned experiment is bred, with plan Nannochloropsis oculata（2300±210 mg/
L）, 5-10 ppm PUFA mixed solution strengthen the artemia just hatched and Copepods 5-8 hours, with fresh water flush bait, according to
Artemia：Copepods=4：6 ratio feeds the hippocampus seedling just hatched, feeds 6-8 times daily, is satiated with food and feeds, residual bait exists
Drained after feeding 2 hours from culturing pool.
After hippocampus seedling is fed 15 days and 30 days, fat content and PUFA assays in hippocampus seedling body are carried out, and
Calculate the hippocampus seedling percent and specific growth rate in 30 days.
Comparative example 1
With reference to the method for embodiment 1, blank control group is set, hippocampus parental source is to be selected with 1 equal batch of embodiment
Hippocampus parent, in blank control group, hippocampus parent feeds the frost oppossum shrimp do not strengthened and 15 age in days shrimps, remaining method
With step with embodiment 1.
Hippocampus seedling derives from above-mentioned blank control group（Do not strengthen）In hippocampus parent bred, hippocampus seedling feeds not
The artemia of reinforcing and Copepods（Feeding volume is 4：6）, remaining method and steps is the same as embodiment 1.
Comparative example 2
Method described in reference implementation example 1, the method that fatty peracid strengthening is carried out just for parent, plantlet stage without
Fortification operates, and hippocampus parental source is the hippocampus parent selected with 1 equal batch of embodiment, and remaining method and steps is the same as real
Apply example 1.
Comparative example 3
Method described in reference implementation example 1, the method that fatty peracid strengthening is carried out just for hippocampus seedling, parent's stage is not
Carry out fortification operation, hippocampus parental source is the hippocampus parent that is selected with 1 equal batch of embodiment, remaining method and steps
With embodiment 1.
Comparative example 4
With reference to the method for embodiment 1, the difference is that the bait that parent's stage strengthens is 15 age in days shrimps, hippocampus parent comes
Source is the hippocampus parent selected with 1 equal batch of embodiment, and remaining method and steps is the same as embodiment 1.
Comparative example 5
With reference to the method for embodiment 1, the difference is that the bait that parent's stage strengthens is shrimp and frost oppossum shrimp, hippocampus parent
Source is the hippocampus parent selected with 1 equal batch of embodiment, and remaining method and steps is the same as embodiment 1.
Comparative example 6
With reference to the method for embodiment 1, the difference is that the bait that parent's stage strengthens is for frost oppossum shrimp, hippocampus parental source
The hippocampus parent selected with 1 equal batch of embodiment, remaining method and steps is the same as embodiment 1.
Comparative example 7
With reference to the method for embodiment 1, the difference is that the bait that hippocampus plantlet stage is strengthened is wheel animalcule and Copepods, hippocampus parent
This source is the hippocampus parent selected with 1 equal batch of embodiment, and remaining method and steps is the same as embodiment 1.
Comparative example 8
With reference to the method for embodiment 1, the difference is that the bait that hippocampus plantlet stage is strengthened is Copepods and fairy shrimp, hippocampus
Parental source is the hippocampus parent selected with 1 equal batch of embodiment, and remaining method and steps is the same as embodiment 1.
Comparative example 9
With reference to the method for embodiment 1, the difference is that the mixed solution that hippocampus plantlet stage is strengthened is chlorella and SUPER
SELCO nutrition fortifiers, hippocampus parental source are the hippocampus parent that is selected with 1 equal batch of embodiment, remaining method and steps
With embodiment 1.
Table 1 below is that embodiment 1 and comparative example 1 ~ 9 determine average fat content in obtained hippocampus seedling（15d and 30d）With
Average PUFA contents（15d and 30d）.The fat content that wherein A is represented in hippocampus seedling body accounts for hippocampus dry weight（10 groups are averaged
Value, 15d, %）, the fat content that B is represented in hippocampus seedling body accounts for hippocampus dry weight（10 cell means, 30d, %）, C represents hippocampus
PUFA contents in seedling body account for internal total lipid content（10 cell means, 15d, %）, D represents the PUFA in hippocampus seedling body
Content accounts for internal total lipid content（10 cell means, 30d, %）.
Table 1
| A | B | C | D | |
| Embodiment 1 | 0.85 | 1.11 | 21.7 | 26.1 |
| Comparative example 1 | 0.61 | 0.91 | 19.0 | 21.1 |
| Comparative example 2 | 0.72 | 1.02 | 19.4 | 22.3 |
| Comparative example 3 | 0.73 | 1.05 | 20.0 | 22.8 |
| Comparative example 4 | 0.75 | 1.02 | 20.2 | 23.3 |
| Comparative example 5 | 0.76 | 1.05 | 20.5 | 23.8 |
| Comparative example 6 | 0.74 | 1.09 | 20.2 | 21.9 |
| Comparative example 7 | 0.77 | 1.05 | 20.8 | 22.5 |
| Comparative example 8 | 0.78 | 1.08 | 20.9 | 22.3 |
| Comparative example 9 | 0.72 | 0.95 | 19.8 | 21.8 |
Table 2 is the hippocampus seedling percent and specific growth rate in 30 days in embodiment 1 and comparative example 1 ~ 9, is each
The average value of experimental group.
Table 2
| The survival rate of hippocampus seedling（30d, %） | Specific growth rate（30d, %） | |
| Embodiment 1 | 93.5 | 19.8 |
| Comparative example 1 | 57.9 | 17.4 |
| Comparative example 2 | 80.9 | 17.9 |
| Comparative example 3 | 81.8 | 18.3 |
| Comparative example 4 | 84.2 | 18.5 |
| Comparative example 5 | 85.1 | 18.4 |
| Comparative example 6 | 82.3 | 18.2 |
| Comparative example 7 | 84.2 | 18.6 |
| Comparative example 8 | 84.5 | 18.7 |
| Comparative example 9 | 80.8 | 18.0 |
With reference to Tables 1 and 2 data, it can be seen that average fat content in the hippocampus seedling of embodiment 1（15d and 30d）With
Average PUFA contents（15d and 30d）, and hippocampus seedling percent in 30 days and specific growth rate reached preferable effect
Fruit, especially, the survival rate of hippocampus seedling breach 90%, reach 93.5%.
With reference to the data of comparative example 1 and 2, it can be seen that only only carried out in parent's stage or hippocampus seedling breeding stage
Fortification, it can not reach the effect of the embodiment of the present invention 1.
In addition, also there is certain influence in the specific bait composition trophic structure of the present invention, only to the culture efficiency of hippocampus
Have on the basis of the frost oppossum shrimp that parent's stage of the present invention is strengthened and 15 age in days shrimps, carried out with reference to hippocampus plantlet stage
The artemia of reinforcing and Copepods are fed, and be can be only achieved unsaturated fatty acid content and fat in higher hippocampus seedling body and are contained
Amount, and it is obviously improved survival rate and specific growth rate.
Meanwhile the nutrition fortifier that the present invention uses also has influence to feeding effect, in the SUPER that the present invention uses
SELCO and plan Nannochloropsis oculata are combined as hardening agent, bait reinforcing are carried out, than SUPER SELCO and the reinforcing effect of chlorella
More preferably.
Claims (4)
1. a kind of aliphatic acid compensation method of hippocampus seedling, it is characterised in that methods described step is as follows：
S1. hippocampus parent is screened；
S2. frost oppossum shrimp and 15 age in days shrimps are soaked respectively using bait hardening agent, feed parent daily 3 times；
S3. the hippocampus parent fed in S2 is mated, after breeding out hippocampus seedling, is placed in other young pond seedling and cultivates；
S4. using intending the mixed solution immersion hippocampus seedling bait of Nannochloropsis oculata and bait hardening agent, and feed to just being incubated in S3
The hippocampus seedling of change, feed daily 6 ~ 8 times；
The hippocampus seedling bait is the artemia and Copepods just hatched；
In S2 during immersion frost oppossum shrimp, it is 8 ~ 15ppm that bait, which strengthens agent concentration,；Soak time is 1 ~ 1.5h；
When 15 age in days shrimps are soaked in S2, it is 5 ~ 10ppm that bait, which strengthens agent concentration,；Soak time is 4 ~ 7h；
In the mixed solution for intending Nannochloropsis oculata and bait hardening agent described in S4, intend Nannochloropsis oculata concentration be 1450 ~ 3600mg/
L, the concentration of bait hardening agent is 5 ~ 10ppm；The bait hardening agent is Super Selco；
Artemia described in S4 and the mixed proportion of Copepods are 2~4：6~8.
2. the aliphatic acid compensation method of the hippocampus seedling according to claim 1, it is characterised in that mixing is molten described in S4
The time of liquid immersion hippocampus seedling bait is 5 ~ 8h.
3. the aliphatic acid compensation method of hippocampus seedling according to claim 1, it is characterised in that artemia described in S4 and oar
The mixed proportion of sufficient class is 4：6.
4. the aliphatic acid compensation method of hippocampus seedling according to claim 1, it is characterised in that hippocampus parent is screened in S1
To select 4 ~ 5 monthly ages health, body surface is mellow and full, and ingest active hippocampus.
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| 海马人工养殖技术研究进展;席寅峰等;《现代渔业信息》;20111025(第10期);第9-15页 * |
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