CN105028269B - A kind of method for culturing seedlings of sturgeon - Google Patents
A kind of method for culturing seedlings of sturgeon Download PDFInfo
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- CN105028269B CN105028269B CN201510357345.7A CN201510357345A CN105028269B CN 105028269 B CN105028269 B CN 105028269B CN 201510357345 A CN201510357345 A CN 201510357345A CN 105028269 B CN105028269 B CN 105028269B
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- 238000000034 method Methods 0.000 title claims abstract description 33
- 238000012258 culturing Methods 0.000 title claims abstract description 18
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- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 18
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 18
- 210000004681 ovum Anatomy 0.000 claims abstract description 18
- 238000002347 injection Methods 0.000 claims abstract description 16
- 239000007924 injection Substances 0.000 claims abstract description 16
- 230000000366 juvenile effect Effects 0.000 claims abstract description 12
- 210000000582 semen Anatomy 0.000 claims abstract description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 11
- 230000009027 insemination Effects 0.000 claims abstract description 11
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 11
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- 230000000694 effects Effects 0.000 claims abstract description 7
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000012528 membrane Substances 0.000 claims abstract description 6
- 230000001817 pituitary effect Effects 0.000 claims description 17
- 230000037396 body weight Effects 0.000 claims description 14
- 241000252233 Cyprinus carpio Species 0.000 claims description 10
- 230000000384 rearing effect Effects 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 7
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 5
- 240000004510 Agastache rugosa Species 0.000 claims description 5
- 240000002234 Allium sativum Species 0.000 claims description 5
- 241000205585 Aquilegia canadensis Species 0.000 claims description 5
- 235000019743 Choline chloride Nutrition 0.000 claims description 5
- 235000019733 Fish meal Nutrition 0.000 claims description 5
- 235000013717 Houttuynia Nutrition 0.000 claims description 5
- 240000000691 Houttuynia cordata Species 0.000 claims description 5
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- FQPFAHBPWDRTLU-UHFFFAOYSA-N aminophylline Chemical compound NCCN.O=C1N(C)C(=O)N(C)C2=C1NC=N2.O=C1N(C)C(=O)N(C)C2=C1NC=N2 FQPFAHBPWDRTLU-UHFFFAOYSA-N 0.000 claims description 5
- 229960003556 aminophylline Drugs 0.000 claims description 5
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 5
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 5
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 5
- 229960003178 choline chloride Drugs 0.000 claims description 5
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 5
- 239000004467 fishmeal Substances 0.000 claims description 5
- 235000004611 garlic Nutrition 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 5
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 230000001568 sexual effect Effects 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 244000241257 Cucumis melo Species 0.000 claims description 4
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims description 4
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims description 4
- 239000004459 forage Substances 0.000 claims description 2
- 229940088594 vitamin Drugs 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 229930003231 vitamin Natural products 0.000 claims description 2
- 235000013343 vitamin Nutrition 0.000 claims description 2
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 2
- 230000004720 fertilization Effects 0.000 abstract description 5
- 238000011534 incubation Methods 0.000 abstract description 4
- 230000017448 oviposition Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 40
- 235000013601 eggs Nutrition 0.000 description 11
- 230000008569 process Effects 0.000 description 9
- 108010010803 Gelatin Proteins 0.000 description 6
- 229920000159 gelatin Polymers 0.000 description 6
- 239000008273 gelatin Substances 0.000 description 6
- 235000019322 gelatine Nutrition 0.000 description 6
- 235000011852 gelatine desserts Nutrition 0.000 description 6
- 230000035800 maturation Effects 0.000 description 6
- 239000002994 raw material Substances 0.000 description 5
- 210000000481 breast Anatomy 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 235000019742 Vitamins premix Nutrition 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000004886 process control Methods 0.000 description 3
- 206010062767 Hypophysitis Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010013911 Dysgeusia Diseases 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 241001560086 Pachyrhizus Species 0.000 description 1
- 206010039984 Senile osteoporosis Diseases 0.000 description 1
- 235000021167 banquet Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000007177 brain activity Effects 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a kind of method for culturing seedlings of sturgeon, it is to catch and select male and female parent population from cultivating pool, parent population is placed in culture pond and cultivated, before artificial propagation is carried out, water temperature is raised, adjust dissolved oxygen in water, and feed feed, expanded in dam's fish belly portion, lower abdomen is soft, cloacal aperture is ruddy, and milter is when gently pressing the belly to have a small amount of milky seminal fluid to flow out, its heat is promoted to female parent population and male parent population injection estrualization agent, from the upper collecting semen of male parent population, ovum is taken in 60 minutes in dam's steck ovum, using semidry method artificial insemination, and embryonated egg is put into talcum powder liquid and stirred, embryonated egg is placed in the incubator with agitating function and hatched, continue to cultivate in incubator after juvenile fish membrane, then juvenile fish is transferred in pond and raised.The method for culturing seedlings of the present invention can not only be such that the egg laying amount of female parent population and ovum quality also greatly increases, and the rate of fertilization of ovum and the incubation rate of embryonated egg greatly improve, significant effect.
Description
Technical field
The present invention relates to fish culture technical field, it particularly relates to a kind of method for culturing seedlings of sturgeon.
Background technology
Sturgeon is cartilage ganoid, is time immemorial fish, the title for having living fossil, and its form is unique, and body is tapered, head, trunk
For a plane, five-element's hone lamella is worn and with sharp spine just as armor, trip such as shuttle are quiet such as ship, body black gray expandable, belly white, wry tail
Shape, ornamental value are very high.Sturgeon is the fabulous large-scale economic fish of edibility.Sturgeon whole body is all precious, and utilization rate is high, removes
Outside sturgeon, its fish maw, fish nose, fish muscle, fish-bone etc. can make the Chinese famous dish of unique style, be first-class delicacies, after the meal
Enjoy endless aftertastes, frequently appear on the dining table of state banquet；Fish-skin can glue, while be also high-grade leather material；Material is done with fish-bone
Manufactured high calcium ticbit, there is unique medical value, to preventing senile osteoporosis, enhancing body immunity, improving
Brain activity, promote health it is highly beneficial.In recent decades, due to the original of the human factors such as environmental pollution and overfishing
Cause, the heavy damage ecosystem of sturgeon, causes some sturgeon species endangered.In order to save these endangered species,
China has started the new upsurge of sturgeon cultivation, and meets the market demand, is particularly achieved in terms of foreign exchange earning bigger
Economic benefit.During the artificial culture of existing sturgeon fry, due to being limited by technology, cause the rate of fertilization of ovum compared with
Low, the incubation rate of embryonated egg is relatively low.
The content of the invention
In view of this, it is an object of the invention to provide a kind of method for culturing seedlings of sturgeon, to solve the rate of fertilization of existing sturgeon
Low, the low and existing deficiency of incubation rate.
The present invention solves above-mentioned technical problem by the following technical programs：
A kind of method for culturing seedlings of sturgeon, comprises the following steps：
(1) parent population selects：Caught from cultivating pool and select physical strong, body surface is without wound, the normal male and female parent of activity
Fish；
(2) parent fish rearing：Parent population is placed in the culture pond that water temperature is 8~12 DEG C and cultivated, 40 before artificial propagation is carried out
~60 days, water temperature is increased to 12~16 DEG C, regulation dissolved oxygen in water is 8~12mg/L, and the forage volume fed daily is parent population body
The 0.7~0.9% of weight；
(3) promote the sexual maturity：Dam's fish belly portion expands, lower abdomen is soft, cloacal aperture is ruddy, and milter gently presses belly to have a small amount of breast
During white seminal fluid outflow, its heat is promoted to female parent population and male parent population injection estrualization agent；
(4) artificial insemination：From the upper collecting semen of male parent population, ovum is taken in 60 minutes in dam's steck ovum, using semidry method
Artificial insemination, and embryonated egg is put into 50~60min of stirring in 20~25% talcum powder liquid；
(5) hatch：Embryonated egg is placed in the incubator with agitating function and hatched, water temperature is 12~16 DEG C during hatching,
The content of dissolved oxygen is 8~9mg/L, and brooding time is 125~145h；
(6) seed rearing：Continue to cultivate 5~6 days in incubator after juvenile fish membrane, then juvenile fish is transferred in pond
Raising.
The body weight of the female parent population is 14~16kg, and the body weight of the male parent population is 20~24kg.
The feed be in parts by weight, by 16~24 parts of the broken leaf of tealeaves, 1~2 part of calcium dihydrogen phosphate, aminophylline 1.2~
1.8 parts, it is 14~20 parts of dregs of beans, 4~8 parts of fly maggot, 40~50 parts of gelatinized starch, 40~60 parts of fish meal, 10~14 parts of cordate houttuynia, wild
8~12 parts of pachyrhizus stem, 2~3 parts of garlic, 1.2~1.8 parts of Choline Chloride, 4~8 parts of honeysuckle, 1~3 part of salt, vitamin are pre-
6~12 parts of 1~2 part of batch mixing, 8~16 parts of radix scutellariae and wrinkled giant hyssop are prepared.
In the step (2), the cultivation density of parent population is 6~8kg/m3。
In the step (3), the estrualization agent injection volume of female parent population is 18~24mg estrualization agents/female parent populations of kg, male parent population
Estrualization agent injection volume is 9~12mg estrualization agents/kg hero parent populations.
The estrualization agent is sturgeon pituitary and carp pituitary.
The sturgeon pituitary and carp weight ratio pituitary are 1：(0.8~1).
The beneficial effects of the present invention are：The method for culturing seedlings of the present invention can not only make the egg laying amount of female parent population increase
Add, than original increase by 10~15%, ovum quality is also greatly increased, and the fertilization rate reached more than 91.8% of ovum, embryonated egg is incubated
Rate more than 70.3%, significant effect.
Embodiment
In order to facilitate the understanding of those skilled in the art, below in conjunction with embodiment, the present invention will be further described.
Embodiment is only for example, not being limitation of the invention, the step of not illustrated in embodiment to the invention
It is prior art, is not described in detail herein.
Embodiment one
A kind of method for culturing seedlings of sturgeon, comprises the following steps：
(1) parent population selects：Caught from cultivating pool and select physical strong, body surface is without wound, the normal male and female parent of activity
Fish, and the body weight of female parent population is 14kg, the body weight of male parent population is 20kg；
(2) parent fish rearing：Parent population is placed in the culture pond that water temperature is 8 DEG C and cultivated, the cultivation density of parent population is 6kg/m3,
40 days before artificial propagation is carried out, water temperature is increased to 12~16 DEG C, regulation dissolved oxygen in water is 8mg/L, the feed fed daily
Measure as the 0.7% of parent population body weight；
(3) promote the sexual maturity：Dam's fish belly portion expands, lower abdomen is soft, cloacal aperture is ruddy, and milter gently presses belly to have a small amount of breast
During white seminal fluid outflow, its heat is promoted to female parent population and male parent population injection estrualization agent, the estrualization agent injection volume of female parent population is 18mg
The female parent populations of estrualization agent/kg, the estrualization agent injection volume of male parent population is 9mg estrualization agents/kg hero parent populations, and the estrualization agent is that sturgeon brain hangs down
Body and carp pituitary, sturgeon pituitary and carp weight ratio pituitary are 1：0.8；
(4) artificial insemination：From the upper collecting semen of male parent population, ovum is taken in 60 minutes in dam's steck ovum, using semidry method
Artificial insemination, and embryonated egg is put into 20% talcum powder liquid and stirs 50min；
(5) hatch：Embryonated egg is placed in the incubator with agitating function and hatched, water temperature is 12 DEG C during hatching, dissolving
The content of oxygen is 8mg/L, brooding time 125h；
(6) seed rearing：Continue to cultivate 5 days in incubator after juvenile fish membrane, then juvenile fish is transferred in pond and raised
Support.
The raw material proportioning of the feed is：The broken leaf 16kg of tealeaves, calcium dihydrogen phosphate 1kg, aminophylline 1.2kg, dregs of beans 14kg,
Fly maggot 4kg, gelatinized starch 40kg, fish meal 40kg, cordate houttuynia 10kg, wild country melon stem 8kg, garlic 2kg, Choline Chloride 1.2kg, gold
Honeysuckle flower 4kg, salt 1kg, vitamin premix 1kg, radix scutellariae 8kg and wrinkled giant hyssop 6kg.Above-mentioned material is respectively fed to dryer
In, moisture is dried at a temperature of 150 DEG C as 8%；Then above-mentioned material is respectively fed to be surpassed in ultra micro container
Crushing of Ultrafine, comminuting matter is set to cross 0.2mm sieves；Then material is sent into tank diameter, regulation temperature is 90 DEG C, stirring reaction
8min；Then mixed material is sent into modulator, the temperature of curing is 110 DEG C, and maturing process control material moisture is
25%, steam pressure 0.5MPa, maturation process 2.5min；Material material after maturation process is sent into extruder and carried out
Expanding treatment；Material after expanding treatment is dried to moisture as 4% at a temperature of 80 DEG C；Thing after multiple baking is handled
Little particle of the material crusher in crushing into 8mm；Material after break process is sent into drum-type flush coater, using 10% content
Gelatin even application in surface of material, the dosage of gelatin is the 2.5% of material, and the material of acquisition is packed, produced.
Embodiment two
A kind of method for culturing seedlings of sturgeon, comprises the following steps：
(1) parent population selects：Caught from cultivating pool and select physical strong, body surface is without wound, the normal male and female parent of activity
Fish, and the body weight of female parent population is 16kg, the body weight of male parent population is 24kg；
(2) parent fish rearing：Parent population is placed in the culture pond that water temperature is 12 DEG C and cultivated, the cultivation density of parent population is 8kg/
m3, 60 days before artificial propagation is carried out, water temperature is increased to 16 DEG C, regulation dissolved oxygen in water is 12mg/L, the feed fed daily
Measure as the 0.9% of parent population body weight；
(3) promote the sexual maturity：Dam's fish belly portion expands, lower abdomen is soft, cloacal aperture is ruddy, and milter gently presses belly to have a small amount of breast
During white seminal fluid outflow, its heat is promoted to female parent population and male parent population injection estrualization agent, the estrualization agent injection volume of female parent population is 24mg
The female parent populations of estrualization agent/kg, the estrualization agent injection volume of male parent population is 12mg estrualization agents/kg hero parent populations, and the estrualization agent is sturgeon brain
Hypophysis and carp pituitary, sturgeon pituitary and carp weight ratio pituitary are 1：1；
(4) artificial insemination：From the upper collecting semen of male parent population, ovum is taken in 60 minutes in dam's steck ovum, using semidry method
Artificial insemination, and embryonated egg is put into 25% talcum powder liquid and stirs 60min；
(5) hatch：Embryonated egg is placed in the incubator with agitating function and hatched, water temperature is 16 DEG C during hatching, dissolving
The content of oxygen is 9mg/L, brooding time 145h；
(6) seed rearing：Continue to cultivate 6 days in incubator after juvenile fish membrane, then juvenile fish is transferred in pond and raised
Support.
The raw material proportioning of the feed be the broken leaf 24kg of tealeaves, calcium dihydrogen phosphate 2kg, aminophylline 1.8kg, dregs of beans 20kg,
Fly maggot 8kg, gelatinized starch 50kg, fish meal 60kg, cordate houttuynia 14kg, wild country melon stem 12kg, garlic 3kg, Choline Chloride 1.8kg,
Honeysuckle 8kg, salt 3kg, vitamin premix 2kg, radix scutellariae 16kg and wrinkled giant hyssop 12kg.Above-mentioned raw materials are respectively fed to dry
In machine, moisture is dried at a temperature of 150 DEG C as 8%；Then above-mentioned material is respectively fed to carry out in ultra micro container
Ultramicro grinding, comminuting matter is set to cross 0.2mm sieves；Then material is sent into tank diameter, regulation temperature is 90 DEG C, stirring reaction
8min；Then mixed material is sent into modulator, the temperature of curing is 110 DEG C, and maturing process control material moisture is
25%, steam pressure 0.5MPa, maturation process 2.5min；Material material after maturation process is sent into extruder and carried out
Expanding treatment；Material after expanding treatment is dried to moisture as 4% at a temperature of 80 DEG C；Thing after multiple baking is handled
Little particle of the material crusher in crushing into 8mm；Material after break process is sent into drum-type flush coater, using 10% content
Gelatin even application in surface of material, the dosage of gelatin is the 2.5% of material, and the material of acquisition is packed, produced.
Embodiment three
A kind of method for culturing seedlings of sturgeon, comprises the following steps：
(1) parent population selects：Caught from cultivating pool and select physical strong, body surface is without wound, the normal male and female parent of activity
Fish, and the body weight of female parent population is 15kg, the body weight of male parent population is 22kg；
(2) parent fish rearing：Parent population is placed in the culture pond that water temperature is 10 DEG C and cultivated, the cultivation density of parent population is 7kg/
m3, 50 days before artificial propagation is carried out, water temperature is increased to 14 DEG C, regulation dissolved oxygen in water is 10mg/L, the feed fed daily
Measure as the 0.8% of parent population body weight；
(3) promote the sexual maturity：Dam's fish belly portion expands, lower abdomen is soft, cloacal aperture is ruddy, and milter gently presses belly to have a small amount of breast
During white seminal fluid outflow, its heat is promoted to female parent population and male parent population injection estrualization agent, the estrualization agent injection volume of female parent population is 20mg
The female parent populations of estrualization agent/kg, the estrualization agent injection volume of male parent population is 10mg estrualization agents/kg hero parent populations, and the estrualization agent is sturgeon brain
Hypophysis and carp pituitary, sturgeon pituitary and carp weight ratio pituitary are 1：0.9；
(4) artificial insemination：From the upper collecting semen of male parent population, ovum is taken in 60 minutes in dam's steck ovum, using semidry method
Artificial insemination, and embryonated egg is put into 22% talcum powder liquid and stirs 54min；
(5) hatch：Embryonated egg is placed in the incubator with agitating function and hatched, water temperature is 14 DEG C during hatching, dissolving
The content of oxygen is 8mg/L, brooding time 130h；
(6) seed rearing：Continue to cultivate 5 days in incubator after juvenile fish membrane, then juvenile fish is transferred in pond and raised
Support.
The raw material proportioning of the feed is the broken leaf 18kg of tealeaves, calcium dihydrogen phosphate 1.5kg, aminophylline 1.5kg, dregs of beans
17kg, fly maggot 6kg, gelatinized starch 45kg, fish meal 50kg, cordate houttuynia 12kg, 10 parts of wild country melon stem, garlic 2.5kg, Choline Chloride
1.5kg, honeysuckle 6kg, salt 2kg, vitamin premix 1.5kg, radix scutellariae 12kg and wrinkled giant hyssop 8kg.Above-mentioned raw materials are distinguished
It is sent into dryer, moisture is dried at a temperature of 150 DEG C as 8%；Then above-mentioned material is respectively fed into ultra micro to hold
Ultramicro grinding is carried out in device, comminuting matter is crossed 0.2mm sieves；Then material is sent into tank diameter, regulation temperature is 90 DEG C, is stirred
Mix reaction 8min；Then mixed material is sent into modulator, the temperature of curing is 110 DEG C, maturing process control material moisture
Content is 25%, steam pressure 0.5MPa, maturation process 2.5min；Material material after maturation process is sent into extruder
Middle carry out expanding treatment；Material after expanding treatment is dried to moisture as 4% at a temperature of 80 DEG C；Multiple baking is handled
Little particle of the material crusher in crushing afterwards into 8mm；Material after break process is sent into drum-type flush coater, used
For the gelatin even application of 10% content in surface of material, the dosage of gelatin is the 2.5% of material, the material of acquisition is packed, i.e.,
.
Tested using the method for culturing seedlings of the embodiment of the present invention three in Furong River proving ground, obtain 42.1 ten thousand ovum
Son, after it is mixed with sperm, 39.2 ten thousand embryonated eggs are obtained, fertilization rate reached 93.1%, hatch 27.9 ten thousand sturgeon childrens altogether
Fish, incubation rate is up to 71.2%.
It is described above, only it is the preferable example of the present invention, any formal limitation not is made to the present invention, it is any not take off
From technical solution of the present invention content, any simple modification made according to technical spirit of the invention to above example, conversion material
Equivalent variations and modification are expected, in the range of still falling within technical solution of the present invention.
Claims (6)
1. a kind of method for culturing seedlings of sturgeon, it is characterised in that comprise the following steps：
(1) parent population selects：Caught from cultivating pool and select physical strong, body surface is without wound, the normal male and female parent population of activity；
(2) parent fish rearing：Parent population is placed in the culture pond that water temperature is 8~12 DEG C and cultivated, 40~60 before artificial propagation is carried out
My god, water temperature is increased to 12~16 DEG C, regulation dissolved oxygen in water is 8~12mg/L, and the forage volume fed daily is parent population body weight
0.7~0.9%, the feed is in parts by weight, by 16~24 parts of the broken leaf of tealeaves, 1~2 part of calcium dihydrogen phosphate, aminophylline 1.2
~1.8 parts, 14~20 parts of dregs of beans, 4~8 parts of fly maggot, 40~50 parts of gelatinized starch, 40~60 parts of fish meal, 10~14 parts of cordate houttuynia,
8~12 parts of wild country melon stem, 2~3 parts of garlic, 1.2~1.8 parts of Choline Chloride, 4~8 parts of honeysuckle, 1~3 part of salt, vitamin
6~12 parts of 1~2 part of premix, 8~16 parts of radix scutellariae and wrinkled giant hyssop are prepared；
(3) promote the sexual maturity：Dam's fish belly portion expands, lower abdomen is soft, cloacal aperture is ruddy, and milter gently presses belly to have a small amount of milky
When seminal fluid flows out, its heat is promoted to female parent population and male parent population injection estrualization agent；
(4) artificial insemination：From the upper collecting semen of male parent population, ovum is taken in 60 minutes in dam's steck ovum, it is artificial using semidry method
Insemination, and embryonated egg is put into 50~60min of stirring in 20~25% talcum powder liquid；
(5) hatch：Embryonated egg is placed in the incubator with agitating function and hatched, water temperature is 12~16 DEG C during hatching, dissolving
The content of oxygen is 8~9mg/L, and brooding time is 125~145h；
(6) seed rearing：Continue to cultivate 5~6 days in incubator after juvenile fish membrane, then juvenile fish is transferred in pond and raised
.
2. the method for culturing seedlings of sturgeon as claimed in claim 1, it is characterised in that the body weight of the female parent population is 14~16kg,
The body weight of the male parent population is 20~24kg.
3. the method for culturing seedlings of sturgeon as claimed in claim 1, it is characterised in that in the step (2), the cultivation density of parent population
For 6~8kg/m3。
4. the method for culturing seedlings of sturgeon as claimed in claim 1, it is characterised in that in the step (3), the estrualization agent of female parent population
Injection volume is 18~24mg estrualization agents/female parent populations of kg, and the estrualization agent injection volume of male parent population is 9~12mg estrualization agents/kg hero parent populations.
5. the method for culturing seedlings of the sturgeon as described in claim 1 or 4, it is characterised in that the estrualization agent be sturgeon pituitary and
Carp pituitary.
6. the method for culturing seedlings of sturgeon as claimed in claim 5, it is characterised in that the sturgeon pituitary and carp are pituitary
Weight ratio is 1：(0.8~1).
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| CN106069899B (en) * | 2016-06-15 | 2021-12-28 | 中国水产科学研究院长江水产研究所 | Method for realizing natural mating and oviposition of Acipenser dabryanus through artificial regulation |
| CN106069900B (en) * | 2016-06-15 | 2017-10-24 | 北京渔夫水产技术开发中心 | A kind of method of Europe huso sturgeon artificial propagation and carp application pituitary |
| CN106172124A (en) * | 2016-07-15 | 2016-12-07 | 杭州千岛湖鲟龙科技股份有限公司 | A kind of artificial breeding method of Europe, southern area huso sturgeon |
| CN106508746A (en) * | 2016-11-07 | 2017-03-22 | 王�华 | Method for artificially breeding parabramis pekinensis |
| CN106719170B (en) * | 2016-12-30 | 2020-08-11 | 贵州锦润水产品有限责任公司 | Sturgeon hatching method |
| CN107549063A (en) * | 2017-09-28 | 2018-01-09 | 临汾市尧都区云盛水产养殖有限公司 | A kind of sturgeon artificial propagation tank and propagation method |
| CN107581107A (en) * | 2017-09-28 | 2018-01-16 | 临汾市尧都区云盛水产养殖有限公司 | A kind of sturgeon propagation method |
| CN108338096A (en) * | 2018-01-30 | 2018-07-31 | 平南县正达农业发展有限公司 | A method of improving carp Quality of Seedlings |
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| JP2006174776A (en) * | 2004-12-22 | 2006-07-06 | Fujikin Inc | Method for breeding sturgeon using identification tool, apparatus for breeding the same, and system for breeding the sturgeon |
| RU2332844C1 (en) * | 2006-11-27 | 2008-09-10 | Федеральное государственное унитарное предприятие "Научно-производственный центр по осетроводству "БИОС" | Way of early lifetime diagnostics of sex of sturgeon fish species and their hybrids |
| CN101518213B (en) * | 2008-02-25 | 2011-01-26 | 中国水产科学研究院东海水产研究所 | High efficient culturing process of Chinese sturgeon |
| KR100979406B1 (en) * | 2010-03-11 | 2010-09-01 | 전라남도 | Sea water acclimation and culture method of rainbow trout and salmon |
| TWM453356U (en) * | 2012-11-28 | 2013-05-21 | Stone & L Resource Industry R & D Ct | Sturgeon roe incubation device |
| CN104160986B (en) * | 2014-06-11 | 2016-08-24 | 贵州省水产研究所 | A kind of mountain area sturgeon parent culture and induced spawning method |
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