CN105016805A - Organic fertilizer prepared by using stems and leaves of lonicera japonica thunb. and artemisia palustris Linn. - Google Patents
Organic fertilizer prepared by using stems and leaves of lonicera japonica thunb. and artemisia palustris Linn. Download PDFInfo
- Publication number
- CN105016805A CN105016805A CN201510447686.3A CN201510447686A CN105016805A CN 105016805 A CN105016805 A CN 105016805A CN 201510447686 A CN201510447686 A CN 201510447686A CN 105016805 A CN105016805 A CN 105016805A
- Authority
- CN
- China
- Prior art keywords
- organic fertilizer
- basic material
- glucose
- fertilizer according
- linn
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
Abstract
The invention provides an organic fertilizer prepared by using stems and leaves of lonicera japonica thunb. and artemisia palustris Linn., relating to a novel organic fertilizer with excellent effects. The fertilizer is prepared by using stems and leaves of lonicera japonica thunb. and artemisia palustris Linn. in a mass ratio of 5 to 1 as basic materials and adopting a solid fermentation method. The organic fertilizer can achieve the effects of obviously increasing the yields of crops and controlling sclerotinia rot of colza to a certain extent.
Description
Technical field
The present invention relates to fertilizer field, especially organic fertilizer technical field.
Background technology
Organic substance in organic fertilizer and nutritive substance are all very abundant, can not only provide required nutrient, can also improve crop yield, improve crop quality, preservation of fertility for the growth of crop, can improve utilization rate of fertilizer simultaneously, reduce production cost.
Along with the raising of people's living standard, the demand of free of contamination organic, the green food of safety and sanitation is in continuous increase, and vast farmers improves the market competitiveness of agricultural-food in the urgent need to application of organic fertilizers.At present, western countries' organic fertilizer consumptions such as the U.S. have accounted for 50% of total amount, and China's Organic fertilizer industry merchandized handling begins to take shape, and using on a large scale of farmland is not yet universal, and institute's accounting is less than 10%.
Rape is the main oil crops of China, and sclerotinia rot of colza is one of important disease in Rape-seed production, and long-term strain sickness rate is up to 10% ~ 30%, and serious reaches more than 80%; The general underproduction 10% ~ 70% of diseased plant.
The Pathogenic Strains sclerotinite (Sclerotinia sclerotiorum (Lib.) de Bary) of sclerotinia rot of colza, belongs to Ascomycotina fungi.Sclerotium Long Circle, to irregular shape, like mouse excrement shape, grays after first white, inner canescence.Grow 1 after sclerotial germination to the meat tawny plate-like apothecium of multiple tool long handle, on dish raw one deck ascus and lateral filament, the colourless club-like of ascus, include the colourless thecaspore of unit cell 8, lateral filament is colourless, thread, half-cooked between ascus.
Honeysuckle (Lonicera japonica Thunb.) is the perennial half evergreen voluble shrub of Caprifoliaceae.There is distribution in most of China area more, and many areas cultivation and production, wherein produces the most well-known with Henan, Shandong.The clean sorrel of sprout, close by tawny, hard straight rough hair, glandular hairs and the pubescence carried out, bottom Chang Wumao.Leaf papery, avette avette to square round shape, ovum shape lanceolar sometimes, avette or the obovate of rare circle, rarely 1 bluntly to incise to several, long 3-5 (-9.5) centimetre, top point or gradually point, rare blunt, circle or nick lack, and base portion circle or closely heart-shaped, has rough echinid, deep green above, light green below, the usual two sides of sprig upper leaf is all close by short rough hair, and how many inferior leads is often smoothly with Steel Gray below without hair; The long 4-8 millimeter of petiole, close by pubescence.Its flower uses as Flos lonicerae, and the stem branch of band leaf is usually abandoned by as forestry waste, both pollutes environment, and causes again the waste of resource.
Black wormwood artemisia (Artemisia palustris Linn.), composite family, artemisia annual herb plant.Stem Dan Sheng, leaf papery, head inflorescence is subsphaeroidal, and without stalk, panicle, corolla tubulose, flower pesticide is linear, and style and corolla are closely isometric, and achene brown is long avette slightly flat little, the flowering fruit bearing stage 8-11 month.It, usually by as weeds in field process, is not well utilized.
Whiterot fungi (Phanerochaete chrysosporium), bacterium number is: CICC 14076.
Healthy and free from worry wood mould (Trichoderma koningii), bacterium number is: CICC 40108.
Aspergillus niger (Aspergillus niger), bacterium number is: CICC 2157.
In prior art, not yet useful honeysuckle cauline leaf and black wormwood artemisia be the report that main raw material prepares organic fertilizer, more not with the report of this fertilizer control sclerotinia rot of colza.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind ofly can prevent and treat sclerotinia rot of colza and the organic fertilizer of fertility excellence.
In order to solve the problems of the technologies described above, the present invention proposes following technical proposal:
With honeysuckle cauline leaf and the organic fertilizer made of black wormwood artemisia, it is characterized in that, described organic fertilizer be by mass ratio be 5:1 honeysuckle cauline leaf and black wormwood artemisia based on raw material, adopt solid fermentation method preparation and obtain.
Described solid fermentation method comprises the following steps:
(1) strain expanded culture: get that healthy and free from worry wood is mould, whiterot fungi, aspergillus niger, is inoculated in liquid PDA substratum, and shaking table is cultivated and expanded, obtained containing bacterium liquid;
(2) solid medium preparation: get described basic material, pulverize, insert 70-90 DEG C of baking oven inner drying, the ratio adding 100-300g water and 3-5g glucose in every 100g basic material adds water and glucose, mixes, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 20-40mL in every 100g basic material is inoculated, and cultivates 8-12 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Strain expanded culture liquid PDA substratum used is the PDA substratum not adding agar, its preparation method takes 200g potato, clean peeling is cut into small pieces, the 1000ml that adds water boils half hour or steaming and decocting under high pressure 20 minutes, filtered through gauze, then add 10-20g glucose, filtered through gauze while hot after fully dissolving, packing test tube, after about sterilizing, taking-up storage is for subsequent use.
Preferably, described (2) step, the drying temperature of basic material is 80 DEG C.
Preferably, described (2) step, the quality that every 100g basic material adds water is 200g.
Preferably, described (2) step, the quality that every 100g basic material adds glucose is 4g.
Preferably, described (3) step, the volume that every 100g basic material adds containing bacterium liquid is 30mL.
Beneficial effect of the present invention can be proved by following test
Test example 1 manure trial
Material and reagent
Organic fertilizer 1 (being designated as examination group 1) of the present invention, prepares by embodiment 1; Organic fertilizer 2 (being designated as examination group 2) of the present invention, prepares by embodiment 2; Organic fertilizer 3 (being designated as examination group 3) of the present invention, prepares by embodiment 3.
Shanghai heat resistanceheat resistant 605 green vegetables seed.
Test method
Adopt randomized block design method design pot experiment.Get volume identical, the flowerpot that shape is consistent, be divided into for examination group (1,2,3) and blank group.For trying fertilizer according to clay fertilizer than 7:3 and mixing with soil, will irrigate with water after loading flowerpot, as supplying examination group (1,2,3); Blank group does not add fertilizer.Often organize test 10 basin, every basin is sowed 9 Shanghai heat resistanceheat resistant 605 seeds and (is considered the percentage of germination of seed, the point that 3 spacing are identical is fixed in flowerpot, each point puts 3 seeds, thinning after germinateing, each point only retains one and grows, and the consistent seedling of growing way), after planting sprinkle one deck dirt (form through high-temperature firing, prevent seed contamination) alive.
Cultivation Shanghai heat resistanceheat resistant 605 green vegetables of 40 days are plucked and weighs, record the growing state of each group of Shanghai heat resistanceheat resistant 605 green vegetables.
Test-results
Test-results is in table 1, and result shows, organic fertilizer of the present invention can improve the output of crop.
The impact that table 1 organic fertilizer of the present invention grows Shanghai heat resistanceheat resistant 605 green vegetables
| Process | Plant height (cm) | Strain heavy (g) | Degree of development (cm) | The number of blade |
| For examination group 1 | 23.0 | 80.7 | 24.7 | 10.4 |
| For examination group 2 | 21.6 | 82.7 | 23.4 | 11.2 |
| For examination group 3 | 22.3 | 84.5 | 25.3 | 11.7 |
| Blank group | 18.2 | 73.7 | 22.2 | 8.5 |
Test example 2 is on the impact of rape sclerotinite sclerotial germination rate
Material and reagent
Organic fertilizer 1 (being designated as examination group 1) of the present invention, prepares by embodiment 1; Organic fertilizer 2 (being designated as examination group 2) of the present invention, prepares by embodiment 2; Organic fertilizer 3 (being designated as examination group 3) of the present invention, prepares by embodiment 3.
Rape sclerotinite (Sclerotinia sclerotiorum (Lib.) de Bary).
Test method
Adopt randomized block design method design pot experiment.Get volume identical, the flowerpot that shape is consistent, be divided into for examination group (1,2,3) and blank group.For trying fertilizer according to clay fertilizer than 7:3 and mixing with soil, will irrigate with water after loading flowerpot, as supplying examination group (1,2,3); Blank group does not add fertilizer.Often organize test 3 basin, load 21 sclerotinia rot of colza sclerotium, length of embedment 2cm with the little mesh bag 5cm × 5cm of nylon, when 60 days, measure the germination rate of sclerotium with potato sucrose substratum (PDA).
Test-results
Test-results is in table 2, and result shows, organic fertilizer of the present invention significantly can reduce the germination rate of rape nuclear disk sclerotium, has certain preventive and therapeutic effect to sclerotinia rot of colza.
Table 2 organic fertilizer of the present invention is on the impact of nuclear disk sclerotium germination rate
| Process | Germination rate (%) |
| For examination group 1 | 23.9 |
| For examination group 2 | 26.5 |
| For examination group 3 | 24.3 |
| Control group | 40.2 |
In order to better set forth technical scheme of the present invention, below in conjunction with embodiment, the present invention is further illustrated, but protection domain of the presently claimed invention is not limited to the following example.
Embodiment
Embodiment 1
The preparation method of organic fertilizer is as follows:
(1) strain expanded culture: get that healthy and free from worry wood is mould, whiterot fungi, aspergillus niger, is inoculated in liquid PDA substratum, and shaking table is cultivated and expanded, obtained containing bacterium liquid;
(2) solid medium preparation: by quality take 5 parts honeysuckle cauline leaf and 1 part of black wormwood artemisia, pulverize, insert 70 DEG C of baking oven inner dryings, the ratio adding 100g water and 3g glucose in every 100g basic material adds water and glucose, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 20mL in every 100g basic material is inoculated, cultivates 8 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Embodiment 2
The preparation method of organic fertilizer is as follows:
(1) strain expanded culture: get that healthy and free from worry wood is mould, whiterot fungi, aspergillus niger, is inoculated in liquid PDA substratum, and shaking table is cultivated and expanded, obtained containing bacterium liquid;
(2) solid medium preparation: by quality take 5 parts honeysuckle cauline leaf and 1 part of black wormwood artemisia, pulverize, insert 90 DEG C of baking oven inner dryings, the ratio adding 300g water and 5g glucose in every 100g basic material adds water and glucose, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 40mL in every 100g basic material is inoculated, cultivates 12 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Embodiment 3
The preparation method of organic fertilizer is as follows:
(1) strain expanded culture: get that healthy and free from worry wood is mould, whiterot fungi, aspergillus niger, is inoculated in liquid PDA substratum, and shaking table is cultivated and expanded, obtained containing bacterium liquid;
(2) solid medium preparation: by quality take 5 parts honeysuckle cauline leaf and 1 part of black wormwood artemisia, pulverize, insert 80 DEG C of baking oven inner dryings, the ratio adding 200g water and 4g glucose in every 100g basic material adds water and glucose, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 30mL in every 100g basic material is inoculated, cultivates 10 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Claims (6)
1., with honeysuckle cauline leaf and the organic fertilizer made of black wormwood artemisia, it is characterized in that, described organic fertilizer be by mass ratio be 5:1 honeysuckle cauline leaf and black wormwood artemisia based on raw material, adopt solid fermentation method preparation and obtain.
2. a kind of organic fertilizer according to claim 1, it is characterized in that, described solid fermentation method comprises the following steps:
(1) strain expanded culture: get that healthy and free from worry wood is mould, whiterot fungi, aspergillus niger, is inoculated in liquid PDA substratum, and shaking table is cultivated and expanded, obtained containing bacterium liquid;
(2) solid medium preparation: get described basic material, pulverize, insert 70-90 DEG C of baking oven inner drying, the ratio adding 100-300g water and 3-5g glucose in every 100g basic material adds water and glucose, mixes, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 20-40mL in every 100g basic material is inoculated, and cultivates 8-12 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
3. a kind of organic fertilizer according to claim 2, it is characterized in that, described (2) step, the drying temperature of basic material is 80 DEG C.
4. a kind of organic fertilizer according to claim 2, it is characterized in that, described (2) step, the quality that every 100g basic material adds water is 200g.
5. a kind of organic fertilizer according to claim 2, it is characterized in that, described (2) step, the quality that every 100g basic material adds glucose is 4g.
6. a kind of organic fertilizer according to claim 2, it is characterized in that, described (3) step, the volume that every 100g basic material adds containing bacterium liquid is 30mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510447686.3A CN105016805A (en) | 2015-07-24 | 2015-07-24 | Organic fertilizer prepared by using stems and leaves of lonicera japonica thunb. and artemisia palustris Linn. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510447686.3A CN105016805A (en) | 2015-07-24 | 2015-07-24 | Organic fertilizer prepared by using stems and leaves of lonicera japonica thunb. and artemisia palustris Linn. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105016805A true CN105016805A (en) | 2015-11-04 |
Family
ID=54407173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510447686.3A Pending CN105016805A (en) | 2015-07-24 | 2015-07-24 | Organic fertilizer prepared by using stems and leaves of lonicera japonica thunb. and artemisia palustris Linn. |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105016805A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649654A (en) * | 2012-04-25 | 2012-08-29 | 孟凡尧 | Traditional Chinese medicine organic fertilizer |
| CN103274771A (en) * | 2013-05-31 | 2013-09-04 | 上海创博生态工程有限公司 | Straw-decomposing inoculant and preparation method thereof |
| CN103449863A (en) * | 2013-08-01 | 2013-12-18 | 湖北诺克特药业有限公司 | Method for producing organic fertilizer by composting and quickly fermenting traditional Chinese medicine dregs |
| CN104692956A (en) * | 2015-01-31 | 2015-06-10 | 浙江农林大学 | Special fertilizer for anoectochilus roxburghii and preparation method of special fertilizer |
-
2015
- 2015-07-24 CN CN201510447686.3A patent/CN105016805A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649654A (en) * | 2012-04-25 | 2012-08-29 | 孟凡尧 | Traditional Chinese medicine organic fertilizer |
| CN103274771A (en) * | 2013-05-31 | 2013-09-04 | 上海创博生态工程有限公司 | Straw-decomposing inoculant and preparation method thereof |
| CN103449863A (en) * | 2013-08-01 | 2013-12-18 | 湖北诺克特药业有限公司 | Method for producing organic fertilizer by composting and quickly fermenting traditional Chinese medicine dregs |
| CN104692956A (en) * | 2015-01-31 | 2015-06-10 | 浙江农林大学 | Special fertilizer for anoectochilus roxburghii and preparation method of special fertilizer |
Non-Patent Citations (4)
| Title |
|---|
| 司华等: "油菜茵核病田间残留菌核萌发规律及研究初报", 《汉中科技》 * |
| 曹翠玲等: "康氏木霉代谢物对植物病原真菌的抗菌活性测定", 《陕西农业科学》 * |
| 谢立华等: "促进作物秸秆和菌核腐解的复合生物制剂应用效果", 《中国油料作物学报》 * |
| 郭新春: "38种中药提取物对4种植物病原菌抑制活性的初步研究", 《中国优秀硕士学位论文全文数据库•农业科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101940116B (en) | Technical method for culturing gastrodia elata at one time under forest | |
| CN107148880B (en) | Planting method of high-calcium organic rice | |
| CN100549158C (en) | A kind of campelyco and preparation method thereof | |
| CN104885745B (en) | A kind of interplanting method of balloonflower root and cowpea | |
| CN105746295A (en) | Quick cutting rooting method of grape | |
| CN105815143A (en) | Seedling-raising matrix for cultivating strong pepper seedlings | |
| CN101773046B (en) | Groundcherry seedling propagation method | |
| CN109265265A (en) | Peanut cultivation special bio bacterial manure and preparation method thereof | |
| CN102061330B (en) | Method for identifying pathogenicity of sesame stem blight and blast pathogenic bacteria | |
| CN108651195A (en) | A method of improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability | |
| CN104585014A (en) | Improved variety soybean breeding method | |
| CN102217657B (en) | Tomato virus disease inhibitor and preparation method thereof | |
| CN107567982A (en) | A kind of breeding method of watermelon seedling | |
| CN106631339A (en) | Special planting substrate capable of increasing dragon fruit yield and preparation method of special planting substrate | |
| CN109644820A (en) | A kind of nutrient soil that plantation sweet potato uses | |
| CN105493880A (en) | Green and high-yield tea-oil tree-tea tree-sweet potato-tarragon mixed planting method | |
| KR20100084080A (en) | Method for cultivating ginseng by organic farming and manufacturing the cultivated ginseng as a extract | |
| CN105925266B (en) | Chinese herbal medicine bactericide suitable for improving sandy soil of peanut field | |
| CN105016805A (en) | Organic fertilizer prepared by using stems and leaves of lonicera japonica thunb. and artemisia palustris Linn. | |
| CN105152697A (en) | Organic fertilizer prepared from solidago canadensis and platanus orientalis leaves | |
| CN105016793A (en) | Organic fertilizer prepared by using stems and leaves of fruit of towel gourd and balsam pear | |
| CN105016795A (en) | Organic fertilizer prepared from solidago Canadensis and cinnamomum camphora leaf | |
| CN105110824A (en) | Organic fertilizer prepared from leaf of Sapium discolor and leaf of white poplar | |
| CN109662103A (en) | A kind of composite biological pesticidal preparations for preventing and treating fruit tree ring spot | |
| CN109169120A (en) | A kind of careless composite plant method of pulse family Qiao and its verification method based on allelopathy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151104 |
|
| RJ01 | Rejection of invention patent application after publication |