CN105013813A - Method for biologically remediating crude oil contaminated soil by using edible fungi residues - Google Patents
Method for biologically remediating crude oil contaminated soil by using edible fungi residues Download PDFInfo
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- CN105013813A CN105013813A CN201510385989.7A CN201510385989A CN105013813A CN 105013813 A CN105013813 A CN 105013813A CN 201510385989 A CN201510385989 A CN 201510385989A CN 105013813 A CN105013813 A CN 105013813A
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Abstract
The invention discloses a method for biologically remediating crude oil contaminated soil by using edible fungi residues. The method comprises the following specific steps: fungi sticks are crushed to obtain fungi residues; enzyme production inducing liquid is added for cultivating under proper conditions to obtain a fermentation ripening system; then, the fermentation ripening system is added in the crude oil contaminated soil in a ratio of 1: 1-3: 1; and dual functions of microbial remediation and phytoremediation can be realized through the degradation of ligninase in a fungi residue fermentation system to phenolic compounds and organic fertilizers provided by the system. Compared with other chemical remediation and physical remediation methods, the method is low in cost and free of secondary pollution, and can improve the efficiency of the biological remediation method.
Description
Technical field
Utilize a method for the biological prosthetic oil contaminated soil of edible fungus bran, be specifically related to add product enzyme inducer to edible fungus bran and oil contaminated soil is repaired.Belong to technical field of bioengineering.
Background technology
The complex mixture that oil is made up of the material that thousands of kinds of chemical property are different, mainly comprises saturated hydrocarbons, compound fragrant hydrocarbon, asphalitine, resinae etc.The pollution of the exploitation of oil, smelting, use and transportation and omission accident, and the discharge of oily waste water, sewage irrigation, the volatilization of various petroleum product, imperfect combustion thing to descend slowly and lightly etc. and cause a series of soil petroleum pollution problem.The ground crude oil that particularly oil exploitation process produces, has become the important sources of soil mineral oil pollution.Some petroleum hydrocarbons enter after in animal body, have carcinogenic, teratogenesis, mutagenic effect to mammal and the mankind.The severe contamination of soil can cause some composition of petroleum hydrocarbon to accumulate in grain, affects the quality of grain, and by food chain, harm humans is healthy.
The reparation of current oil contaminated soil mainly comprises chemical means, physical means and biological means.Bioremediation technology is a kind of emerging clean technologies grown up on biodegradable basis, and it is the development of traditional bioremediation.Compared with physics, chemical redemption contaminated soil technology, it is low that it has cost, do not destroy the soil environment required for plant growth, pollutant oxidative safety, non-secondary pollution, the features such as treatment effect is good, simple to operate.Biological prostheticly accelerating natural biology degradation rate by the optimization of environmental factor, is the clean technologies that a kind of efficient, economy and ecology can be born.At present, the bioremediation technology administering petroleum hydrocarbon contaminated soil mainly contains two classes: a class is microorganism remediation technology; Another kind of is phytoremediation.
Summary of the invention:
The object of the present invention is to provide a kind of method utilizing the biological prosthetic oil contaminated soil of edible fungus bran, the bacterium rod containing hypha of edible fungus is utilized to be raw material, add wherein and produce enzyme induction liquid promotion edible mushroom making full use of biomass material, and then obtain lignoenzyme and the bacterium chaff residue compared with low ratio of carbon to ammonium.By the fertilizer that lignoenzyme in bacterium chaff fermentation system provides the degraded of phenolic compound and system, the double action of microorganism remediation and phytoremediation can be realized.Greatly improve the efficiency of biological prosthetic means.
Bacterium rod refers to cotton seed hulls, wood chip, straw, corncob, bagasse and multiple kinds of crops stalk, industrial waste for primary raw material, is used for the bar-shaped solid mixt producing fruit body of edible fungi or fructification of gathering is later remaining after inoculation hypha of edible fungus cultivates a period of time.When hypha of edible fungus grows in the medium, need to produce a large amount of lignoenzyme to decompose lignocellulosic in matrix to obtain nutrition.Therefore, results or not yet gather in the crops fructification bacterium rod in containing a large amount of hypha of edible fungus.The concrete composition of bacterium rod can not as the restriction to reparation oil contaminated soil method of the present invention.
Industrial waste is mainly selected from vinegar grain, vinasse, paper mill effluent.
Edible mushroom is the artificial mushroom cultivated or can be under wild environment biology and eat.Lignoenzyme can be secreted to decompose lignocellulosic in matrix to obtain nutrition in its growth course.Described edible mushroom is mainly selected from mushroom (Agaricus campestris), mushroom (Lentinula edodes), agrocybe (Agrocybe aegerita), Hericium erinaceus (Hericium erinaceum), phoenix-tail mushroom (Pleurotus sajor-caju), agaricus bisporus (Agaricusbisporus), auricularia auriculajudae (Auricularia auricula), Cordyceps sinensis (Cordyceps sinensis), glossy ganoderma (Ganoderma lucidum), pycnoporus samguineus (Pycnoporus sanguineus), gold ear (Tremellaaurantialba), grey mushroom (abalonelike) (Pleurotus abalonus), flat mushroom (Pleurotus ostreatus), asparagus (Flammulina velutipes), pleurotus eryngii (Pleurotus eryngii), elegant precious mushroom (Pleurotus geesteranus), coprinus comatus (Coprinus comatus), Pleurotus nebrodensis (Pleurotus nebrodensis), lung shape picks up the ears (Pleurotuspulmonarius), Agricus blazei (Agaricus blazei) etc.
Technical scheme of the present invention:
The invention provides a kind of method utilizing the biological prosthetic oil contaminated soil of edible fungus bran, specifically comprise the steps: that the break process of bacterium rod obtains bacterium chaff; Add and produce enzyme induction liquid; Cultivate under suitable condition; By fermenting, the ratio of maturation system according to 1: 1-3: 1 joins in the soil by crude oil pollution; Hyperaccumulative plant is planted in mixed system.
1. broken bacterium rod obtains bacterium chaff
After the bacterium of fructification of or not yet gathering rod removing polybag, utilize pulverizer that the fragmentation of bacterium rod is obtained uniform bacterium chaff.
2. add induction and produce enzyme liquid
The inducer that enzyme liquid contains lignoenzyme is produced in induction used, and other substances provide nitrogen element and trace element for hypha of edible fungus, can also adjust carbon-nitrogen ratio (C/N) and the pH value of system in addition, stimulates mycelia diauxic growth.Induction is produced enzyme liquid and is obtained by the prolonged and repeated test in this laboratory.
A kind of concrete embodiment, the usual addition that enzyme liquid is produced in every 10kg bacterium chaff induction is 10-50L, 10-30L can be selected further, the diauxic growth that more effectively can stimulate hypha of edible fungus in bacterium chaff when 12,13,15,16,17,19,20,23,25 or 28L is selected, the high expression of induction lignoenzyme and secretion when concrete.
3. cultivate under suitable condition
Suitable extraneous condition of culture can change the physiological status of hypha of edible fungus, better expression and secretion lignoenzyme, and then biomass material is fully degraded.
A concrete embodiment, cultivation temperature is generally 17-36 DEG C, can be chosen as 22-30 DEG C further, or is specifically chosen as 22,24,25,27,28,30 DEG C.The time of cultivating under above temperature conditions is 24-168 hour, or selects 24,72,96,120 or 144 hours further, can obtain that lignoenzyme content is higher, lignocellulosic transforms bacterium chaff more thoroughly.
4. the mixing of bacterium chaff fermentation system and contaminated soil
What obtain to step 3 obtains mixed system containing joining in contaminated soil with the ratio of 1: 1-3: 1 in the fermentation maturation system of a large amount of lignoenzyme.Utilize the lignoenzyme of higher concentration in fermentation maturation system to carry out biodegradation to the aldehydes matter in soil, stir weekly once.
5. carry out phytoremediation to mixed system inoculation hyperaccumulative plant
Obtain to step 4 plant that mixed system inoculation has super summation or acid-fast alkali-proof effect, utilize plant to repair the accumulation of pollutant and metabolic conversion.
Beneficial effect of the present invention:
The invention provides a kind of method utilizing the biological prosthetic oil contaminated soil of edible fungus bran.Be a kind of new way of bacterium chaff efficiency utilization, both can have reduced edible fungus bran and arbitrarily be discharged into the pollution problem that environment causes, and twice laid environmental protect can have been accomplished again, there is good application prospect.
Detailed description of the invention:
Below in conjunction with embodiment, the present invention is described further.Following examples are only a specific embodiment of the present invention, but design concept of the present invention is not limited thereto, and all changes utilizing this design the present invention to be carried out to unsubstantiality, all should belong to the behavior of invading scope.
Embodiment
Get 1kg pleurotus eryngii bacterium rod; fragmentation adds the product enzyme induction liquid of 1.5L after obtaining bacterium chaff; cultivate 5 days at 23 DEG C; 2kg contaminated soil is added in fermentation maturation system; mix, stir weekly once, the morning glory with super summation that in a month backward mixed system, inoculation 20 strain laboratory screenings obtain; plant after five months and record the rising of soil with organic matter concentration, crude oil concentration, polycyclic aromatic hydrocarbon concentration all decline to a great extent.
Claims (6)
1. one kind utilizes the method for the biological prosthetic oil contaminated soil of edible fungus bran, be characterized in that the bacterium chaff to obtain after edible fungus stick fragmentation is raw material, add product enzyme inducer, fermentation maturation system is joined in the soil by crude oil pollution, by the fertilizer that lignoenzyme in bacterium chaff fermentation system provides the degraded of phenolic compound and system, can realize the double action of microorganism remediation and phytoremediation, the method comprises the following steps:
A) by after the bacterium of fructification of or not yet gathering rod removing polybag, utilize pulverizer that the fragmentation of bacterium rod is obtained uniform bacterium chaff;
B) in obtained bacterium chaff, add product enzyme induction liquid according to the ratio of solid-liquid mass volume ratio 1: 1-4, after mixing, obtain fermentation system;
C) fermentation system is cultivated 24-168 hour under 17-36 DEG C of condition, carry out fermenting and producing lignocellulolyticenzymes;
D) the fermentation maturation system obtained joins in the soil by crude oil pollution according to the ratio of 1: 1-3: 1.
2. utilize a method for the biological prosthetic oil contaminated soil of edible fungus bran, it is characterized in that described product enzyme induction formula of liquid is: in mass, urea is 0.1 ~ 5%, ammonium nitrate is 0.2 ~ 6%.
3. utilize a method for the biological prosthetic oil contaminated soil of edible fungus bran, it is characterized in that every 10kg bacterium rod needs the product enzyme induction liquid of 10L-40L.
4. utilize a method for the biological prosthetic oil contaminated soil of edible fungus bran, it is characterized in that cultivation temperature is 17-36 DEG C.
5. utilize a method for the biological prosthetic oil contaminated soil of edible fungus bran, it is characterized in that incubation time is 24-168 hour.
6. utilize a method for the biological prosthetic oil contaminated soil of edible fungus bran, it is characterized in that fermentation system and be 1: 1-3: 1 by the soil volume ratio of crude oil pollution.
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| CN201510385989.7A CN105013813A (en) | 2015-07-01 | 2015-07-01 | Method for biologically remediating crude oil contaminated soil by using edible fungi residues |
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| CN201510385989.7A CN105013813A (en) | 2015-07-01 | 2015-07-01 | Method for biologically remediating crude oil contaminated soil by using edible fungi residues |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107297389A (en) * | 2017-08-09 | 2017-10-27 | 爱土工程环境科技有限公司 | A kind of biomass medicament and its application, the restorative procedure of contaminated soil |
| CN112209783A (en) * | 2020-11-05 | 2021-01-12 | 上海市农业科学院 | Method for improving farmland soil by using stropharia rugoso-annulata mushroom dregs |
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| CN101780465A (en) * | 2010-03-30 | 2010-07-21 | 中国科学院南京土壤研究所 | Method for remediating polycyclic aromatic hydrocarbon-polluted soil by jointly enhancing plants through edible fungus residue and biosurfactant |
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| CN101433906A (en) * | 2008-12-25 | 2009-05-20 | 南开大学 | Method for repairing oil polluted soil using ornamental plant morning-glory |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107297389A (en) * | 2017-08-09 | 2017-10-27 | 爱土工程环境科技有限公司 | A kind of biomass medicament and its application, the restorative procedure of contaminated soil |
| CN112209783A (en) * | 2020-11-05 | 2021-01-12 | 上海市农业科学院 | Method for improving farmland soil by using stropharia rugoso-annulata mushroom dregs |
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Application publication date: 20151104 |