CN101780465A - Method for remediating polycyclic aromatic hydrocarbon-polluted soil by jointly enhancing plants through edible fungus residue and biosurfactant - Google Patents
Method for remediating polycyclic aromatic hydrocarbon-polluted soil by jointly enhancing plants through edible fungus residue and biosurfactant Download PDFInfo
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- CN101780465A CN101780465A CN 201010135665 CN201010135665A CN101780465A CN 101780465 A CN101780465 A CN 101780465A CN 201010135665 CN201010135665 CN 201010135665 CN 201010135665 A CN201010135665 A CN 201010135665A CN 101780465 A CN101780465 A CN 101780465A
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Abstract
The invention discloses a method for remediating polycyclic aromatic hydrocarbon-polluted soil by jointly enhancing plants through edible fungus residue and biosurfactant, which comprises the following steps: (1) planting alfalfa in the polycyclic aromatic hydrocarbon-polluted soil at intervals of between 30 and 40cm; (2) when the height of the plant is between 7 and 10cm, applying 10 to 20g of edible fungus residue in dry weight to the soil in the depth of between 5 and 10cm within a range that the diameter of plant root circles is between 5 and 25cm; (3) applying 1 to 3g of biosurfactant in a deep soil layer with 5 to 10 cm, stirring uniformly and covering earth by 3 to 5cm; (4) repeating the step (3) every 60 days; and (5) harvesting the plant bodies of the alfalfa after 120 days, drying in the sun, and transporting to other places for burning. The method can effectively promote PAHs organic pollutants to desorb from soil particles, and improve the bioavailability and degradation rate of the organic pollutants without secondary pollution; and the cost is greatly reduced.
Description
Technical field
The present invention relates to contaminated environment recovery technique field, relate in particular to a kind of method of utilizing edible fungus bran and biosurfactant jointly enhancing plants to repair polycyclic aromatic hydrocarbon pollution.
Background technology
Soil is one of the mankind's most important natural resources of depending on for existence, but because atmospheric sedimentation, sewage irrigation, solid waste landfill seepage and oilfield exploitation etc., the soil organic contamination is on the rise.Polycyclic aromatic hydrocarbon is the organic pollution that a class contains two or two above phenyl ring, has carcinogenic, teratogenesis and mutagenic effect, can move in soil-crop system, and cause the underground water secondary pollution, jeopardizes agricultural product security and health.P in soil AHs pollutes the health that not only influences soil ecosystem, also influence the healthy of the mankind by number of ways such as food chains, therefore P in soil AHs pollutes the extensive attention that is subjected to government and society, and PAHs contaminated soil reparation research has become the research emphasis of international soil environment technical field.
Phytoremediation technology is that of getting up of development in recent years is mainly used in the green recovery technique of removing toxic pollutant in the environment, and it is low, simple to operate to have an investment, and non-secondary pollution can be used for advantages such as large-area pollution control.But because the bioavailability of polycyclic aromatic hydrocarbon is lower in the soil, the efficient of phytoremediation often has certain limitation in a short time, and the degradation rate at a complete plant growth cycle PAHs only is 10%～20% usually.Therefore, need on the basis of plant restoration method, take some enhancements to improve phytoremediation efficient.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing edible fungus bran and biosurfactant jointly enhancing plants to repair polycyclic aromatic hydrocarbon pollution.This method can effectively promote the desorb from the soil particle of PAHs class organic pollution, improves the bioavailability and the degradation rate of such organic pollution, also has environmental friendliness, non-secondary pollution, economy and characteristics such as workable simultaneously.Compared with prior art, the present invention can reduce the cost of environmental improvement significantly.
The scheme of finishing the foregoing invention task is that a kind of method of utilizing edible fungus bran and biosurfactant jointly enhancing plants to repair polycyclic aromatic hydrocarbon pollution is characterized in that step is as follows:
(1) adopt the mode of bunch planting to plant alfalfa in polycyclic aromatic hydrocarbons contaminated soil, the cave spacing is 30～40cm;
When (2) treating that alfalfa grows to plant height 7～10cm, in the dark soil of plant roots loop diameter 5～25cm scope 5～10cm, apply 10～20g (dry weight) edible fungus bran (not needing the secondary compost fermentation), stir with soil;
(3) in the dark soil layer of 5～10cm, apply 1～3g (net content) biosurfactant in soil simultaneously, stir earthing 3～5cm with soil and edible fungus bran;
(4) every 60 days, repeating step (3) once.
(5) plant of results alfalfa after 120 days dries, and transfers to other local burnings
In the above scheme, described edible fungus bran is meant: culture medium waste behind the edible mushrooms such as two spore mushrooms, pleurotus eryngii, coprinus comatus and flat mushroom.Described edible fungus bran adds five times of H
2PH during O is 7.8～8.5, organic carbon 550～600g/kg wherein, and full nitrogen 18～22g/kg, full phosphorus 4～5g/kg, full potassium 8～12g/kg, laccase content is 30000～40000U/kg.
Described biosurfactant is to produce bacterium pseudomonas aeruginosa liquid fermentation gained by rhamnolipid, is sprayed in the soil uniformly according to 1～3g (net content) addition.
Surfactant can effectively promote the desorb from the soil particle of PAHs class organic pollution, realizes the effect of solubilising PAHs, thereby helps to improve the bioavailability and the degradation rate of such organic pollution.Compare with chemical surfactant, rhanolipid as biosurfactant removes the characteristic that possesses surfactant, also has environmental friendliness, non-secondary pollution and economic dispatch characteristics, has very big potential application foreground in the environment remediation field.In addition, rhamnolipid can promote the plant growth in the finite concentration scope.
Edible fungus bran, refer to cultivate the solid waste of being left behind the various edible mushrooms more, it contains the composition that protein, amino acid, mycoprotein, enzyme etc. can utilize again, what particularly comprise laccase, lignin peroxidase and manganese peroxidase enriches the oxidoreducing enzyme resource, can for providing, its extensive use in the environment remediation field provide powerful support for by degrade organic pollution in the environment of redox reaction.In addition, contain necessary for plant growth various nutrient elements such as great number of organic matters and nitrogen, phosphorus, potassium, calcium, magnesium, sulphur in the edible fungus bran, particularly the humic acid organic colloid of its generation can make soil aggregate increase, improve soil characteristic, chesson, increase gas permeability, these provide good soil environment for the plant growth, thereby help the growth of plant.
Utilize the method for edible fungus bran and biosurfactant and use chemical fertilizer and the contrast of chemical surfactant cost: edible fungus bran is the culture medium of edible fungus waste material, price is 40～50 yuan/ton, biosurfactant system is produced by microbial fermentation, the cost that produces rhamnolipid is only for culture medium batching and sweat charges for water and electricity etc. are 2.9～3.8 yuan/L, and rhamnolipid concentration is 3g/L～5g/L.And the price of chemical surfactant Triton X-100 is 100～110 yuan/500ml, 2400～2450 yuan/ton of composite fertilizer'ss (45% content).The alfalfa seed cost is 55～60 yuan/kilogram.Repair 1 hectare of soil, the method cost of edible fungus bran and biosurfactant that utilizes of the present invention needs about 80～100 yuan, and uses chemical fertilizer and chemical surfactant according to the conventional method, be more than 1000 yuan.
The invention has the advantages that: the present invention promotes the plant growth, and then increases rhizosphere soil microorganism quantity and metabolic activity by adding nutrient elements such as a certain proportion of edible fungus bran raising soil organic matter content and nitrogen, phosphorus, potassium.By the polycyclic aromatic hydrocarbon in the direct pollution degradation soil of the laccase in the edible fungus bran.Simultaneously, polycyclic aromatic hydrocarbon desorb from the soil particle is got off, improve the bioavailability and the degradability of organic pollution by biosurfactant.At last, improve quantity and the metabolic activity of edaphon by the root system effect of plant.
Description of drawings
Fig. 1 is among the embodiment 1, [the contrast: do not plant plant of different disposal P in soil AHs degradation rate; Handle 1: the plantation plant; Handle 2: plantation plant+rhamnolipid; Handle 3: plantation plant+rhamnolipid+button mushroom chaff (5g); Handle 4: plantation plant+rhamnolipid+button mushroom chaff (10g); Handle 5: plantation plant+rhamnolipid+button mushroom chaff (20g)];
Fig. 2 is among the embodiment 2, [the contrast: do not plant plant of different disposal P in soil AHs degradation rate; Handle 1: the plantation plant; Handle 2: plantation plant+pleurotus eryngii bacterium chaff; Handle 3: plantation plant+pleurotus eryngii bacterium chaff+rhamnolipid (1g); Handle 4: plantation plant+pleurotus eryngii bacterium chaff+rhamnolipid (2g); Handle 5: plantation plant+pleurotus eryngii bacterium chaff+rhamnolipid (3g); Handle 6: plantation plant+pleurotus eryngii bacterium chaff+rhamnolipid (4g)];
Fig. 3 is among the embodiment 3, [the contrast: do not plant plant of different disposal P in soil AHs degradation rate; Handle 1: the plantation plant; Handle 2: plantation plant+flat mushroom bacterium chaff; Handle 3: plantation plant+rhamnolipid (1g); Handle 4: plantation plant+rhamnolipid (2g); Handle 5: plantation plant+rhamnolipid (4g); Handle 6: plantation plant+flat mushroom bacterium chaff+rhamnolipid (1g); Handle 7: plantation plant+flat mushroom bacterium chaff+rhamnolipid (2g); Handle 8: plantation plant+flat mushroom bacterium chaff+rhamnolipid (4g)].
Specifically buy the mode of executing
Embodiment 1: contain the contaminated soil of polycyclic aromatic hydrocarbon total amount 10.31mg/kg, with bunch planting way plantation alfalfa, planting the plant hole spacing is 40cm, and the management method according to common crops behind plant survival manages; When treating that plant grows to plant height 10cm, in soil, apply button mushroom chaff 0g respectively, 5g, 10g and 20g, simultaneously, applying 0.5L concentration respectively in soil is the 3g/L rhamnolipid fermentation liquor, mix about the earthing 2cm of back, gather pedotheque after cultivating 120d, and analyze polycyclic aromatic hydrocarbon content variation in the soil, estimate repairing effect.
Cross 100 mesh sieves after the soil collection, take by weighing 1g soil and add the 2g anhydrous Na simultaneously
2SO
4Abundant mixing, after adding the 60ml carrene, behind the cable-styled extraction 24h, use the Rotary Evaporators evaporate to dryness, use 2ml cyclohexane constant volume then, get the chromatographic column that 0.5ml crosses 60-100 order silica gel (activating with n-hexane), (1: 1, V/V) wash-out and dry up with nitrogen was settled to high performance liquid chromatograph analysis behind the 2ml with acetonitrile to carrene/n-hexane at last.
Behind the 120d, the degradation rate data of soil polycyclic aromatic hydrocarbon total amount are seen shown in Figure 1, wherein, the degradation rate of not planting the plant contrast only is 2.1%, the degradation rate 12.5% of independent phytoremediation (handling 1), the degradation rate of plantation plant and rhamnolipid synergy (handling 2) brings up to 19.8%, add different amount (5g on this basis, 10g and 20g) the degradation rate handled of button mushroom chaff reached 30.9%, 32.64% and 21.2% respectively, improved 147%, 161% and 70% than the independent remediation efficiency of plant.Therefore on the basis of button mushroom chaff addition 5～10g, add the reparation of rhamnolipid 1.5g fortification of plants simultaneously and can reach effect preferably.
Embodiment 2:
The contaminated soil that contains polycyclic aromatic hydrocarbon total amount 8.87mg/kg, with bunch planting way plantation alfalfa, planting the plant hole spacing is 35cm, the management method according to common crops behind plant survival manages; When treating that plant grows to plant height 12cm, in soil, apply pleurotus eryngii bacterium chaff 10g, simultaneously, applying 0.25L, 0.5L, 0.75L and 1L concentration in soil is the 4g/L rhamnolipid fermentation liquor, mix about the earthing 2cm of back, cultivate and gather pedotheque after 120 days, and analyze polycyclic aromatic hydrocarbon content variation in the soil, estimate repairing effect.
Cross 100 mesh sieves after the soil collection, take by weighing 1g soil and add the 2g anhydrous Na simultaneously
2SO
4Abundant mixing, after adding the 60ml carrene, behind the cable-styled extraction 24h, use the Rotary Evaporators evaporate to dryness, use 2ml cyclohexane constant volume then, get the chromatographic column that 0.5ml crosses 60～100 order silica gel (activating with n-hexane), (1: 1, V/V) wash-out and dry up with nitrogen was settled to high performance liquid chromatograph analysis behind the 2ml with acetonitrile to carrene/n-hexane at last.
Behind the 120d, the degradation rate data of soil polycyclic aromatic hydrocarbon total amount are seen shown in Figure 2, wherein, the degradation rate of not planting the plant contrast only is 2.4%, the degradation rate 13.1% of independent phytoremediation (handling 1), the degradation rate of plantation plant and the synergy of pleurotus eryngii bacterium chaff (handling 2) brings up to 21.5%, add different amount (1g on this basis, 2g, 3g and 4g) the degradation rate of rhamnolipid fermentation liquor reached 32.8%, 37.59%, 36.95% and 25.3% respectively, improved 53%, 75%, 72% and 18% than the independent remediation efficiency of plant.Therefore on the basis of pleurotus eryngii bacterium chaff addition 10g, add the reparation of rhamnolipid 1～3g fortification of plants simultaneously and can reach effect preferably.
Embodiment 3:
The contaminated soil that contains polycyclic aromatic hydrocarbon total amount 13.27mg/kg, with bunch planting way plantation alfalfa, planting the plant hole spacing is 40cm, the management method according to common crops behind plant survival manages; When treating that plant grows to plant height 12cm, in soil, apply flat mushroom bacterium chaff 10g, simultaneously, applying 0.25L, 0.5L and 0.75L concentration in soil is the 4g/L rhamnolipid fermentation liquor, mix about the earthing 2cm of back, establish the contrast that does not apply flat mushroom bacterium chaff and apply the various dose rhamnolipid fermentation liquor simultaneously.Gather pedotheque after cultivating 120d, and analyze polycyclic aromatic hydrocarbon content variation in the soil, estimate repairing effect.
Behind the 120d, the degradation rate data of soil polycyclic aromatic hydrocarbon total amount are seen shown in Figure 3.Wherein, the degradation rate of not planting the plant contrast only is 2.6%, the degradation rate 15.1% of independent phytoremediation (handling 1), the degradation rate of plantation plant and the synergy of flat mushroom bacterium chaff (handling 2) brings up to 22.4%, does not apply flat mushroom bacterium chaff and the contrast PAHs degradation rate that applies the various dose rhamnolipid fermentation liquor is respectively 17.9% (1g rhamnolipid), 17.1% (2g rhamnolipid) and 20.3% (4g rhamnolipid).And the degradation rate that applies flat mushroom bacterium chaff and the rhamnolipid fermentation liquor that adds different amounts (1g, 2g and 4g) has simultaneously reached 38.59%, 40.95% and 30.01% respectively, has improved 71%, 82% and 33% than the independent remediation efficiency of plant.Therefore on the basis of flat mushroom bacterium chaff addition 10g, add the reparation of rhamnolipid 1～2g fortification of plants simultaneously and can reach effect preferably.
Claims (5)
1. method of utilizing edible fungus bran and biosurfactant jointly enhancing plants to repair polycyclic aromatic hydrocarbon pollution is characterized in that step is as follows:
(1) adopt the mode of bunch planting to plant alfalfa in polycyclic aromatic hydrocarbons contaminated soil, the cave spacing is 30～40cm;
When (2) treating that alfalfa grows to plant height 7～10cm, in the dark soil of plant roots loop diameter 5～25cm scope 5～10cm, apply the edible fungus bran of dry weight 10～20g, stir with soil;
(3) in the dark soil layer of 5～10cm, apply 1～3g biosurfactant simultaneously, stir earthing 3～5cm with soil and edible fungus bran;
(4) every 60 days, repeating step (3) once;
(5) plant of results alfalfa after 120 days dries, and transfers to other local burnings.
2. the method for utilizing edible fungus bran and biosurfactant jointly enhancing plants to repair polycyclic aromatic hydrocarbon pollution according to claim 1, it is characterized in that the edible fungus bran in described (2) step is meant: culture medium waste behind the edible mushrooms such as two spore mushrooms, pleurotus eryngii, coprinus comatus and flat mushroom; Biosurfactant is meant rhamnolipid.
3. the method for utilizing edible fungus bran and biosurfactant jointly enhancing plants to repair polycyclic aromatic hydrocarbon pollution according to claim 1, it is characterized in that, the mode that applies of the edible fungus bran in described (2) step is: diameter is that 5～25cm, the degree of depth are the upper soll layer place of 5～10cm around root system of plant, the edible fungus bran of uniform broadcasting one deck dry weight 10～20g.
4. the method for utilizing edible fungus bran and biosurfactant jointly enhancing plants to repair polycyclic aromatic hydrocarbon pollution according to claim 1, it is characterized in that, described biosurfactant, system produces bacterium pseudomonas aeruginosa liquid fermentation gained by rhamnolipid, is sprayed in the soil uniformly according to 1～3g addition.
5. according to the described method of utilizing edible fungus bran and biosurfactant jointly enhancing plants to repair polycyclic aromatic hydrocarbon pollution of one of claim 1～4, it is characterized in that:
Described edible fungus bran adds five times of H
2PH during O is 7.8～8.5, organic carbon 550～600g/kg wherein, and full nitrogen 18～22g/kg, full phosphorus 4～5g/kg, full potassium 8～12g/kg, laccase content is 30000～40000U/kg.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102553903A (en) * | 2012-01-11 | 2012-07-11 | 中国科学院南京土壤研究所 | Remediation method of heavy metal contaminated soil |
| CN104495971A (en) * | 2014-11-27 | 2015-04-08 | 福建农林大学 | Method for processing aniline-blue-containing pollution wastewater by using cultivation material of harvested pleurotus eryngii |
| CN104694513A (en) * | 2014-12-03 | 2015-06-10 | 江南大学 | Method for producing ligninase by using edible mushroom stick |
| CN104974991A (en) * | 2015-07-09 | 2015-10-14 | 福建农林大学 | Method for preparing solid laccase through pleurotus eryngii waste fungus chaff |
| CN105013813A (en) * | 2015-07-01 | 2015-11-04 | 江南大学 | Method for biologically remediating crude oil contaminated soil by using edible fungi residues |
| CN105032923A (en) * | 2015-07-01 | 2015-11-11 | 江南大学 | Method used for bioremediation of PAHs polluted soil with edible mushroom residue |
| CN106085446A (en) * | 2016-06-17 | 2016-11-09 | 战锡林 | Polycyclic aromatic hydrocarbon pollution repair materials |
| CN108372191A (en) * | 2018-02-02 | 2018-08-07 | 贵州大学 | A kind of method of " plant-enzyme " combination degradation PAHs in soil |
| CN108817054A (en) * | 2018-07-31 | 2018-11-16 | 江苏省环境科学研究院 | A method of polycyclic aromatic hydrocarbon pollution is repaired using plant hormone and biosurfactant fortification of plants |
| CN114345906A (en) * | 2021-11-29 | 2022-04-15 | 贵州大学 | Method for removing polycyclic aromatic hydrocarbon organic pollutants in indoor environment |
| CN116689477A (en) * | 2023-06-25 | 2023-09-05 | 江苏大学 | Bioremediation method of degradation flora composite edible fungus residues of polycyclic aromatic hydrocarbon contaminated soil |
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2010
- 2010-03-30 CN CN2010101356655A patent/CN101780465B/en not_active Expired - Fee Related
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102553903A (en) * | 2012-01-11 | 2012-07-11 | 中国科学院南京土壤研究所 | Remediation method of heavy metal contaminated soil |
| CN104495971A (en) * | 2014-11-27 | 2015-04-08 | 福建农林大学 | Method for processing aniline-blue-containing pollution wastewater by using cultivation material of harvested pleurotus eryngii |
| CN104694513A (en) * | 2014-12-03 | 2015-06-10 | 江南大学 | Method for producing ligninase by using edible mushroom stick |
| CN105013813A (en) * | 2015-07-01 | 2015-11-04 | 江南大学 | Method for biologically remediating crude oil contaminated soil by using edible fungi residues |
| CN105032923A (en) * | 2015-07-01 | 2015-11-11 | 江南大学 | Method used for bioremediation of PAHs polluted soil with edible mushroom residue |
| CN104974991A (en) * | 2015-07-09 | 2015-10-14 | 福建农林大学 | Method for preparing solid laccase through pleurotus eryngii waste fungus chaff |
| CN106085446A (en) * | 2016-06-17 | 2016-11-09 | 战锡林 | Polycyclic aromatic hydrocarbon pollution repair materials |
| CN108372191A (en) * | 2018-02-02 | 2018-08-07 | 贵州大学 | A kind of method of " plant-enzyme " combination degradation PAHs in soil |
| CN108817054A (en) * | 2018-07-31 | 2018-11-16 | 江苏省环境科学研究院 | A method of polycyclic aromatic hydrocarbon pollution is repaired using plant hormone and biosurfactant fortification of plants |
| CN114345906A (en) * | 2021-11-29 | 2022-04-15 | 贵州大学 | Method for removing polycyclic aromatic hydrocarbon organic pollutants in indoor environment |
| CN114345906B (en) * | 2021-11-29 | 2024-04-09 | 贵州大学 | Method for removing polycyclic aromatic hydrocarbon organic pollutants in indoor environment |
| CN116689477A (en) * | 2023-06-25 | 2023-09-05 | 江苏大学 | Bioremediation method of degradation flora composite edible fungus residues of polycyclic aromatic hydrocarbon contaminated soil |
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