CN105012225B - A kind of skin care enzyme liquid and its application - Google Patents
A kind of skin care enzyme liquid and its application Download PDFInfo
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- CN105012225B CN105012225B CN201510451440.3A CN201510451440A CN105012225B CN 105012225 B CN105012225 B CN 105012225B CN 201510451440 A CN201510451440 A CN 201510451440A CN 105012225 B CN105012225 B CN 105012225B
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Abstract
The invention provides a kind of beauty and skin care enzyme liquid and its application.The raw material of the beauty and skin care enzyme liquid includes in parts by weight：1~10 part of malt, 5~10 parts of saccharomycete, every kind of 0.1~3 part of both effectiveness raw material.Four kinds to ten kinds in following component of the both effectiveness raw material：Ginseng, Semen Persicae, almond, Poria cocos, coix seed, fructus lycii, licorice, scutellariae,radix, the root of kudzu vine, root of Rhodiola sachalinensis, bletilla striata root, radix bardanae, rose, dandelion, tealeaves, lophatherum gracile, accumulated snow blade of grass, chrysanthemum, gardenia, purslane, oenothera biennis, hops, motherwort.Ferment facial mask essence containing foregoing beauty and skin care enzyme liquid can help activating skin cells, enhance metabolism, effectively repair aged skin, oxidation resistance is improved, increases skin water content, long-acting water lock moisturizing, nourishing effect is played while moisturizing, makes skin smooth and high resilience.
Description
Technical field
The present invention relates to cosmetics manufacture field, specifically, it is related to a kind of skin care enzyme liquid and its application.
Background technology
Ferment, is a kind of material with special bioactivity being made up of amino acid, is to maintain body normal function, disappears
Helping digestion thing, a kind of required material of the vital movement such as repair tissue.Ferment can bring vigor for Skin Cell, accelerate its new old generation
Thank, also there is decomposition removal effect to dirt, dead cell.Ferment can promote normal cell proliferation and damaged cell to regenerate, and make cell
Get well, skin high resilience, and skin is kept moistening for a long time.Therefore, ferment turns into the emerging cosmetics work(of a class
Imitate additive.
Application No. 201410451181.X patent application discloses a kind of ferment facial mask with moisturizing whitening function
Essence, wherein 4~6 parts of enzyme stoste of addition.The enzyme stoste is formed by fruit spontaneous fermentation, and fruit is filtered off after fermentation ends
Slag produces enzyme stoste, and sterile filling is standby.Fermentation process takes spontaneous fermentation, and easy pollution microbes, fermented quality is unstable,
It is not easily accomplished the production of industrialized standard metaplasia.Not yet explicitly illustrate microbiological treatment mode in enzyme stoste.
Today of popular favor is increasingly subject in facial mask class cosmetics, needing offer one kind badly being capable of activating skin cells, rush
Enter metabolism, effectively repair aged skin, and improve oxidation resistance simultaneously, increase the facial mask essence of skin water content.
The content of the invention
In order to solve problems of the prior art, it is an object of the invention to provide a kind of skin care enzyme liquid and its should
With.
In order to realize the object of the invention, present invention firstly provides a kind of skin care enzyme liquid, its raw material includes：Malt, yeast
Bacterium and both effectiveness raw material；Four kinds to ten kinds in following component of the both effectiveness raw material：Ginseng, Semen Persicae, almond, Fu
Siberian cocklebur, coix seed, fructus lycii, licorice, scutellariae,radix, the root of kudzu vine, root of Rhodiola sachalinensis, bletilla striata root, radix bardanae, rose, dandelion, tea
Leaf, lophatherum gracile, accumulated snow blade of grass, chrysanthemum, gardenia, purslane, oenothera biennis, hops, motherwort.
Preferably, five kinds to seven kinds in said components of the both effectiveness raw material.
Further, its raw material includes in parts by weight：1~10 part of malt, 5~10 parts of saccharomycete, both effectiveness raw material
Every kind of 0.1~3 part.Preferably, every kind of 0.1~1 part of the both effectiveness raw material.
Further, the preparation method of the skin care enzyme liquid comprises the following steps：
(1) both effectiveness raw material is mixed, adds the water immersion of relative 10~20 times of its dry weight or boil, through being filtrated to get leaching
Extract, obtains feed liquid I；
Specifically, ginseng in raw material, Semen Persicae, almond, Poria cocos, coix seed, fructus lycii, licorice, scutellariae,radix, the root of kudzu vine,
Root of Rhodiola sachalinensis, bletilla striata root, radix bardanae etc. add 10~20 times of water of its dry weight when preparing leaching liquor, soak 2~6 hours, boil
20~40min, filters to obtain a leaching liquor, repeat extraction once secondary leaching liquor, control boils intensity and is less than 10%.Raw material
Middle rose, dandelion, tealeaves, lophatherum gracile, accumulated snow blade of grass, chrysanthemum, gardenia, purslane, oenothera biennis, hops, motherwort
Deng when preparing leaching liquor, 10~20 times of water of its dry weight are added, 70~90 DEG C of constant temperature soak 20~40min, filter once to soak
Extract, repeat extraction once secondary leaching liquor.Mix all leaching liquors and obtain feed liquid I；
(2) malt is crushed, crosses and sacchariferous equipment is added after 10~50 mesh sieves, the water of 2~4 times of addition malt dry weight, stage
Temperature reaction to iodine shows completely, adds the poor filter 23 of more than 80 DEG C hot water wash, and each water consumption is 1~2 times of malt dry weight,
Merging filtrate, obtains feed liquid II；
(3) feed liquid I and feed liquid II are squeezed into fermentation tank, is warming up to 120 DEG C, sterilize 30min；
(4) after sterilizing terminates, cooling feed liquid accesses saccharomycete to 25~30 DEG C；
(5) clean compressed air, 25~30 DEG C, 100~1000rpm stirrings fermentation 24~72 hours are passed through；
(6) zymotic fluid is centrifuged after fermentation ends, fermented supernatant fluid and sediment is collected respectively；
(7) fermented supernatant fluid obtained by the step of 2~6 times of its weight in wet base is added in sediment (6), it is scattered mix after carry out it is low
Warm clasmatosis, broken rear liquid obtains yeast lysis thing through centrifuging and taking supernatant；
(8) yeast lysis thing obtained by step (6) remaining fermented supernatant fluid and step (7) is mixed, through the degerming micro-filtration of film
System is filtered, and collects bacteria-free filtrate, as described skin care enzyme liquid.
Wherein, the stage temperature reaction of step (2) is specially：45~50 DEG C of insulation 30min of heating, are warming up to 62~65 DEG C
40min is incubated, 70~72 DEG C is warming up to and shows completely to iodine.
Wherein, the low temperature clasmatosis of step (7) is specially：Carried out using low-temperature ultrahigh-pressure continuous flow cell disintegrating machine thin
Born of the same parents crush.
Wherein, the centrifugal condition of step (6) and step (7) is 8000~12000rpm.
Further, used yeast bacterium is Pichia pastoris (Pichia sp.) and candida valida (Candida
valida).Preferably, Pichia pastoris (Pichia sp.) and candida valida (Candida in used yeast bacterium
Valida 1) is pressed:2~2:1 ratio mixing.
The skin care enzyme liquid that the present invention is provided can be used for being fabricated to cosmetics as functional component.
Invention further provides a kind of ferment facial mask essence, contain foregoing skin care in the ferment facial mask essence
Enzyme liquid, the mass percent of the skin care enzyme liquid is 5~15%.
Preferably, the mass percent of the skin care enzyme liquid is 10%.
Further, the ferment facial mask essence in percentage by weight, including following composition：Water 70~90%；
Skin care enzyme liquid 5~15%；Glycerine 2~4%；Ginseng root extract 1~3%；Butanediol 0.5~3%；Trehalose 0.5~
3%；Sodium Hyaluronate 0.02~0.2；Panthenol 0.5~1.0%；Tocopherol 0.1~0.5%, allantoin 0.2~0.5%, black bearberry
Glycosides 0.2~1%；Cremophor RH40 0.3~1.5%；Carbomer 0.1~0.3%；Glycerine polymethacrylates 0.1
~0.5%；Sodium hydroxide 0.01~0.05%；Methylisothiazolinone 0.005~0.01%；Iodine propilolic alcohol butyl mephenesin Carbamate
0.005~0.02%.
The beneficial effects of the present invention are：
Contain abundant amino acid, oligopeptides, vitamin, mineral matter, ferment and many in the skin care enzyme liquid that the present invention is provided
Plant plant extract component.Plant extraction liquid passes through the fermentation of microorganism, not only remains the nutritional ingredient of itself, also increases
The nutritional ingredient that microbial metabolic products are brought, such as vitamin, amino acid, polypeptide, enzyme.Digest and make in enzyme microb
Under, some nutritive effect compositions of plant extracts are changed into small molecule from macromolecular, and such as protein has been hydrolyzed into amino acid, will
Originally nonabsorbable composition becomes absorbable composition to skin.These compositions are the NMFs in skin, with skin
Compatibility is high and is readily absorbed by.These compositions have the hydrophilic radicals such as many amino, hydroxyl, carboxyl, have very strong knot with water
Cooperation is used, and large quantity of moisture can be carried while cell is entered and enters cell, and can be locked in moisture intracellular and have and protect
Wet function.
Impurity that ferment in skin care enzyme liquid can be cleared up in skin surface rubbish, such as cell surface, intercellular lipid,
The rubbish and the cuticula of overaging being blocked in follicular orifice, sweat gland, sebaceous glands, are that the input of moisture and nutriment is cleared away
Obstacle.With the increase of skin water absorption, cuticula is fully hydrated be swelled after, the increase of osmotic absorption ability, ferment can be inhaled
Receive deeply to skin layers, scavenger-cell metabolism dirt is cleared up, the small circulation of unobstructed skin obtains skin from profound level
Nutrition that must be sufficient and moisture.Ferment and small molecule nutriment in skin care enzyme liquid can not only be skin cell metabolism
More rich nutriment is provided, and with panthenol, vitamin E, Sodium Hyaluronate, ursin, allantoin, ginseng root extract
The effects such as components matching use, these nutrition compositions will be promoted to go deep into flesh bottom and its flesh bottom absorbability is improved, strengthen its function effect
With.
The skin care enzyme liquid provided in the present invention, is formed, fermentation process peace through the preferred specified microorganisms fermentation of intelligent control
Complete controllable, tunning is pollution-free, non-stimulated, safe.Serial low-temperature treatment measure is employed after enzyme liquid fermentation ends,
Somatic cells Dissolve things inside is released, enzyme liquid nutritional ingredient is added, the same of enzyme liquid bioactive ingredients validity is being ensured
When remove wherein microorganism, meet its quality《Cosmetics health specification》Related request.Meanwhile, foundation《Cosmetics health is advised
Model》Carry out acute oral toxicity test, acute dermal toxicity experiment, skin irritation/corrosion test, acute eye irritation/
Corrosion test, as a result shows that the skin care enzyme liquid is nontoxic, nonirritant, non-corrosiveness.
The skin care enzyme liquid provided in the present invention, simple production process is with short production cycle, is easy to artificial intelligence control, can
Large-scale standardized production is carried out, is had a wide range of application.
The ferment facial mask essence that the present invention is provided can help activating skin cells, enhance metabolism, and effectively repair old
Change skin, improve oxidation resistance, increase skin water content, nourishing effect is played in long-acting water lock moisturizing while moisturizing, make
Skin is smooth and high resilience.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Skin care enzyme liquid of embodiment 1 and preparation method thereof
Skin care enzyme liquid raw material in parts by weight, including following component：2 parts of malt, 0.1 part of ginseng, Semen Persicae 0.5
Part, 1 part of coix seed, 0.8 part of tealeaves, 5 parts of saccharomycete.
Skin care ferment liquid and preparation method thereof comprises the following steps：
(1) raw material ginseng, Semen Persicae, coix seed and tealeaves needed for weighing respectively in proportion, clear up removal of impurities.Mixing ginseng,
Semen Persicae, coix seed, add 20 times of water of its dry weight, soak 4 hours, boil 30min, filter to obtain a leaching liquor, repeat to extract
Once secondary leaching liquor, control boils intensity less than 10%.The water of 15 times of its dry weight, 80 DEG C of constant temperature immersions are added in tealeaves
30min, filters to obtain a leaching liquor, repeat extraction once secondary leaching liquor.Mix all leaching liquors and obtain feed liquid I.
(2) dried malt needed for weighing in proportion, clears up removal of impurities, crushes, and sacchariferous equipment is added after crossing 10~50 mesh sieves, adds
The water that 4 times of malt dry weight, heat up 50 DEG C of insulation 30min, is warming up to 65 DEG C of insulation 40min, is warming up to 70 and shows completely to iodine, adds
The poor filter 23 of more than 80 DEG C hot water wash, each water consumption is 1 times of malt dry weight, and merging filtrate obtains feed liquid II.
(3) feed liquid I and feed liquid II are squeezed into fermentation tank, is warming up to 120 DEG C, sterilize 30min.
(4) after sterilizing terminates, cooling feed liquid accesses saccharomycete to 30 DEG C.Used yeast bacterium is Pichia pastoris (Pichia
) and candida valida (Candida valida) sp..Saccharomycete activation culture is that aseptically, saccharomycete is inoculated with
In sterilizing malt extract medium, 30 DEG C, 150rpm is cultivated 36 hours, and yeast count is up to 108Individual/more than ml.Pichia pastoris
(Pichia sp.) and candida valida (Candida valida) inoculative proportion is 1:1.
(5) clean compressed air, 30 DEG C, 120rpm stirrings fermentation 36 hours are passed through.
(6) 10000rpm centrifuges zymotic fluid online after fermentation ends, and fermented supernatant fluid and sediment are collected respectively.
(7) above-mentioned (6) supernatant of 6 times of its weight in wet base is added in sediment, low-temperature ultrahigh-pressure is used after scattered mixing
Continuous flow cell disintegrating machine carries out clasmatosis, and broken rear liquid is centrifuged through 10000rpm, and it is molten that exclusion precipitation takes supernatant to obtain yeast
Born of the same parents' thing.
(8) yeast lysis thing obtained by above-mentioned (6) remaining fermented supernatant fluid and above-mentioned (7) is mixed, through the degerming micro-filtration of film
System is filtered, and is collected bacteria-free filtrate and is obtained skin care enzyme liquid.
Foundation《Cosmetics health specification》Acute oral toxicity test, urgency are carried out to skin care enzyme liquid manufactured in the present embodiment
Property percutaneous toxicity test, skin irritation/corrosion test, acute eye irritation/corrosion test, as a result show the skin care ferment
Plain liquid is nontoxic, nonirritant, non-corrosiveness.
The domestic authoritative institution of commission carries out proteomic image analysis to skin care enzyme liquid manufactured in the present embodiment and detected, as a result
Show to mainly contain the various enzymes shown in table 1 in skin care enzyme liquid manufactured in the present embodiment.
The enzyme contained in the skin care enzyme liquid of table 1
Skin care enzyme liquid of embodiment 2 and preparation method thereof
Skin care enzyme liquid raw material in parts by weight, including following component：5 parts of malt, 0.6 part of bletilla striata root, 1 part of almond,
0.2 part of root of Rhodiola sachalinensis, 0.8 part of dandelion, 1.0 parts of centella, 10 parts of saccharomycete.
Skin care ferment liquid and preparation method thereof comprises the following steps：
(1) raw material bletilla striata root, almond, root of Rhodiola sachalinensis, dandelion, centella needed for weighing respectively in proportion, clear up removal of impurities.
The bletilla striata, almond and root of Rhodiola sachalinensis are mixed, the water of 15 times of its dry weight is added, is soaked 4 hours, is boiled 35min, filter once to extract
Liquid, repeat extraction once secondary leaching liquor, control boil intensity be less than 10%.Dandelion and centella are mixed, it is added and does
Weigh 10 times of water, 75 DEG C of constant temperature soak 40min, filter to obtain a leaching liquor, repeat extraction once secondary leaching liquor.Mixing institute
There is leaching liquor to obtain feed liquid I.
(2) dried malt needed for weighing in proportion, clears up removal of impurities, crushes, and sacchariferous equipment is added after crossing 10~50 mesh sieves, adds
The water that 3 times of malt dry weight, heat up 45 DEG C of insulation 30min, is warming up to 62 DEG C of insulation 40min, is warming up to 72 DEG C and shows completely to iodine, plus
Enter the poor filter 23 of more than 80 DEG C hot water wash, each water consumption is 2 times of malt dry weight, and merging filtrate obtains feed liquid II.
(3) feed liquid I and feed liquid II are squeezed into fermentation tank, is warming up to 120 DEG C, sterilize 30min.
(4) after sterilizing terminates, cooling feed liquid accesses saccharomycete to 28 DEG C.Used yeast bacterium is Pichia pastoris (Pichia
) and candida valida (Candida valida) sp..Saccharomycete activation culture is that aseptically, saccharomycete is inoculated with
In sterilizing malt extract medium, 30 DEG C, 150rpm is cultivated 36 hours, and yeast count is up to 108Individual/more than ml.Pichia pastoris
(Pichia sp.) and candida valida (Candida valida) inoculative proportion is 2:1.
(5) clean compressed air, 28 DEG C, 500rpm stirrings fermentation 30 hours are passed through.
(6) 12000rpm centrifuges zymotic fluid online after fermentation ends, and fermented supernatant fluid and sediment are collected respectively.
(7) above-mentioned (6) supernatant of 5 times of its weight in wet base is added in sediment, low-temperature ultrahigh-pressure is used after scattered mixing
Continuous flow cell disintegrating machine carries out clasmatosis, and broken rear liquid is centrifuged through 12000rpm, and it is molten that exclusion precipitation takes supernatant to obtain yeast
Born of the same parents' thing.
(8) yeast lysis thing obtained by above-mentioned (6) remaining fermented supernatant fluid and above-mentioned (7) is mixed, through the degerming micro-filtration of film
System is filtered, and is collected bacteria-free filtrate and is obtained skin care enzyme liquid.
Foundation《Cosmetics health specification》Acute oral toxicity test, urgency are carried out to skin care enzyme liquid manufactured in the present embodiment
Property percutaneous toxicity test, skin irritation/corrosion test, acute eye irritation/corrosion test, as a result show the skin care ferment
Plain liquid is nontoxic, nonirritant, non-corrosiveness.
Skin care enzyme liquid of embodiment 3 and preparation method thereof
The present embodiment and the difference of embodiment 1 are：
Skin care enzyme liquid raw material in parts by weight, including following component：8 parts of malt, 1.0 parts of scutellariae,radix, Poria cocos 0.5
Part, 0.3 part of fructus lycii, 1.0 parts of purslane, 0.8 part of oenothera biennis, 0.5 part of lophatherum gracile, 8 parts of saccharomycete.
Foundation《Cosmetics health specification》Acute oral toxicity test, urgency are carried out to skin care enzyme liquid manufactured in the present embodiment
Property percutaneous toxicity test, skin irritation/corrosion test, acute eye irritation/corrosion test, as a result show the skin care ferment
Plain liquid is nontoxic, nonirritant, non-corrosiveness.
Ferment facial mask essence of embodiment 4 and preparation method thereof
Ferment facial mask essence prepares raw materials used as shown in table 2.
The ferment facial mask essence of table 2 prepares raw materials used
Ferment facial mask essence preparation method is as follows：
(1) deionized water is weighed in proportion and squeeze into emulsification pot, open stirring, put into load weighted carbomer, stir to complete
Fully dissolved, adds sodium hydroxide solution regulation pH to 5~7.Then load weighted trehalose, glycerine, butanediol, urine are sequentially added
Bursin, ursin, often add a component to add next component to after being completely dissolved, and are stirred after adding to fully transparent equal
It is even.
(2) deionized water is weighed in proportion, load weighted Sodium Hyaluronate is added, and is stirred to being completely dissolved, is then added
Above-mentioned (1) resulting solution.Stirring and homogeneous are opened, 80 DEG C are warming up to, insulated and stirred 30min makes each component fully dissolve mixing.
(3) cooling is opened, above-mentioned (2) resulting solution temperature is down to 40 DEG C, the poly- methyl of load weighted glycerine is sequentially added
Acrylate, panthenol, methylisothiazolinone and iodine propilolic alcohol butyl mephenesin Carbamate, stirring homogeneous are completely dissolved each component.
(4) load weighted tocopherol and Cremophor RH40 are mixed, stirring is mixed to homogeneous transparent, is added above-mentioned
(3) resulting solution.
(5) load weighted skin care enzyme liquid and ginseng root extract are sequentially added in above-mentioned (4) resulting solution, stirring is mixed
It is even.Sampling carries out the inspection of semifinished product, and qualified rear discharging is filling.Final product quality meets QB/T 2872《Facial mask》Related request.
Ferment facial mask essence of embodiment 5 and preparation method thereof
Ferment facial mask essence prepares raw materials used as shown in table 3.
The ferment facial mask essence of table 3 prepares raw materials used
| Composition | Consumption (part) |
| Water | 80.412 |
| Skin care enzyme liquid prepared by embodiment 2 | 10 |
| Ginseng root extract | 2 |
| Panthenol | 1.0 |
| Sodium Hyaluronate | 0.04 |
| Trehalose | 0.5 |
| Butanediol | 1 |
| Glycerine | 3 |
| Tocopherol | 0.2 |
| Allantoin | 0.3 |
| Ursin | 0.6 |
| Cremophor RH40 | 0.6 |
| Carbomer | 0.2 |
| Glycerine polymethacrylates | 0.1 |
| Sodium hydroxide | 0.03 |
| Methylisothiazolinone | 0.008 |
| Iodine propilolic alcohol butyl mephenesin Carbamate | 0.01 |
Ferment facial mask essence preparation method is as follows：
(1) deionized water is weighed in proportion and squeeze into emulsification pot, open stirring, put into load weighted carbomer, stir to complete
Fully dissolved, adds sodium hydroxide solution regulation pH to 5~7.Then load weighted trehalose, glycerine, butanediol, urine are sequentially added
Bursin, ursin, often add a component to add next component to after being completely dissolved, and are stirred after adding to fully transparent equal
It is even.
(2) deionized water is weighed in proportion, load weighted Sodium Hyaluronate is added, and is stirred to being completely dissolved, is then added
Above-mentioned (1) resulting solution.Stirring and homogeneous are opened, 80 DEG C are warming up to, insulated and stirred 30min makes each component fully dissolve mixing.
(3) cooling is opened, above-mentioned (2) resulting solution temperature is down to 45 DEG C, the poly- methyl of load weighted glycerine is sequentially added
Acrylate, panthenol, methylisothiazolinone and iodine propilolic alcohol butyl mephenesin Carbamate, stirring homogeneous are completely dissolved each component.
(4) load weighted tocopherol and Cremophor RH40 are mixed, stirring and dissolving to homogeneous transparent is added above-mentioned
(3) resulting solution.
(5) load weighted skin care enzyme liquid and ginseng root extract are sequentially added in above-mentioned (4) resulting solution, stirring is mixed
It is even.Sampling carries out the inspection of semifinished product, and qualified rear discharging is filling.Final product quality meets QB/T 2872《Facial mask》Related request.
Ferment facial mask essence of embodiment 6 and preparation method thereof
The present embodiment and the difference of embodiment 4 are：Skin care enzyme liquid prepared by embodiment 1 replaces with embodiment 3 and made
Standby skin care enzyme liquid.
Experimental example 1
Ferment facial mask essence prepared by embodiment 4~6 coordinates facial mask cloth compatible to be prepared into mask, respectively
It is each on probation about 2 months through the 100 20-50 Sui women without allergic history, paste within every 4 days 1, amount to 15 and paste.It is required that the face cleaning that washes one's face
Afterwards, by facial mask mulching on the face, facial mask is removed after 15~20 minutes, massage skin is gently patted, make Essence all absorptions, clearly
Water carries out operational maintenance after cleaning.Trial employment period occurs without any allergic phenomena.After end on probation, more than 95 parts triers think
Skin makes moderate progress than before, becomes the tender moistening of water, smooth and flexible.
Ferment facial mask essence of embodiment 7~12 and preparation method thereof
The difference of embodiment 7~12 and embodiment 4 is：The consumption of skin care enzyme liquid is replaced with 0,2%, 5%, 8%,
10%th, 20%, the part of change is consistent total amount by adjusting the consumption of water.
Comparative example 1
Facial mask cloth compatible is coordinated to be prepared into face patch facial mask essence described in embodiment 4 and embodiment 7-12
Film, it is each on probation about 2 months through 20~50 years old women without allergic history respectively, paste within every 4 days 1, amount to 15 and paste.It is required that it is clean to wash one's face
Behind face, by facial mask mulching on the face, facial mask is removed after 15~20 minutes, massage skin is gently patted, make Essence all absorptions,
Clear water carries out operational maintenance after cleaning.Using effect satisfaction investigation is carried out to trier after end on probation, as a result such as table 4 below institute
Show：
The facial mask using effect satisfaction investigation result of table 4
During average mark >=4, facial mask using effect satisfaction is at least up to the level of " fine ", that is, reaches the acceptable water of experiment
It is flat.When differing≤0.2 between average mark, using effect is considered as no significant changes, that is, is considered as same level.
According to facial mask essence using effect satisfaction average mark, with reference to skin care enzyme liquid cost economic benefit, shield is selected
Skin enzyme liquid addition concentration range is 5~15%, and when wherein concentration is 10%, using effect is substantially better than 5% and 8%, with
15% quite, and use cost is less than 15%, therefore 10% and is considered as skin care enzyme liquid and most preferably adds concentration.
Facial mask essence using effect satisfaction average mark is in skin care enzyme liquid addition concentration 0~10% with skin care ferment
The increase of plain liquid concentration and quickly increase, skin care enzyme liquid addition concentration 10%~20% in without significant change, show facial mask
Essence using effect is related to skin care enzyme liquid addition concentration, influences particularly evident in low concentration, high concentration is without obvious shadow
Ring, this is mainly related to skin care enzyme liquid effective efficiency constituent concentration in facial mask essence.During low concentration, functional component can not expire
Needed for skin of foot metabolism, action effect increases and increased with functional component concentration, once functional component concentration increases to foot
Taken with meeting skin cell metabolism, its action effect will be without significant changes.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. a kind of skin care enzyme liquid, it is characterised in that its raw material includes：Malt, saccharomycete and both effectiveness raw material；It is described both effectiveness
Four kinds to ten kinds in following component of raw material：Ginseng, Semen Persicae, almond, Poria cocos, coix seed, fructus lycii, licorice, Huang
A kind of reed mentioned in ancient books root, the root of kudzu vine, root of Rhodiola sachalinensis, bletilla striata root, radix bardanae, rose, dandelion, tealeaves, lophatherum gracile, accumulated snow blade of grass, chrysanthemum, cape jasmine
Flower, purslane, oenothera biennis, hops, motherwort；
The preparation method of the skin care enzyme liquid comprises the following steps：
(1) both effectiveness raw material is mixed, adds the water immersion of relative 10~20 times of its dry weight or boil, through being filtrated to get extraction
Liquid, obtains feed liquid I；
(2) malt is crushed, crosses and sacchariferous equipment is added after 10~50 mesh sieves, the water of 2~4 times of addition malt dry weight, stage heating
Reaction to iodine shows completely, adds the poor filter 23 of more than 80 DEG C hot water wash, and each water consumption is 1~2 times of malt dry weight, is merged
Filtrate, obtains feed liquid II；
(3) feed liquid I and feed liquid II are squeezed into fermentation tank, is warming up to 120 DEG C, sterilize 30min；
(4) after sterilizing terminates, cooling feed liquid accesses saccharomycete to 25~30 DEG C；
(5) clean compressed air, 25~30 DEG C, 100~1000rpm stirrings fermentation 24~72 hours are passed through；
(6) zymotic fluid is centrifuged after fermentation ends, fermented supernatant fluid and sediment is collected respectively；
(7) fermented supernatant fluid obtained by the step of 2~6 times of its weight in wet base is added in sediment (6), disperses after mixing to carry out low temperature thin
Born of the same parents crush, and broken rear liquid obtains yeast lysis thing through centrifuging and taking supernatant；
(8) yeast lysis thing obtained by step (6) remaining fermented supernatant fluid and step (7) is mixed, through the degerming microfiltration systems of film
Filtering, collects bacteria-free filtrate, as described skin care enzyme liquid.
2. skin care enzyme liquid according to claim 1, it is characterised in that its raw material includes in parts by weight：Malt 1~
10 parts, 5~10 parts of saccharomycete, every kind of 0.1~3 part of both effectiveness raw material.
3. skin care enzyme liquid according to claim 1, it is characterised in that the low temperature clasmatosis of step (7) is specially：Adopt
Clasmatosis is carried out with low-temperature ultrahigh-pressure continuous flow cell disintegrating machine.
4. skin care enzyme liquid according to claim 1, it is characterised in that the centrifugal condition of step (6) and step (7) is
8000~12000rpm.
5. skin care enzyme liquid according to claim 1 or 2, it is characterised in that used yeast bacterium is Pichia pastoris and thick shape
Candida.
6. a kind of ferment facial mask essence, it is characterised in that any containing Claims 1 to 5 in the ferment facial mask essence
Skin care enzyme liquid described in, the mass percent of the skin care enzyme liquid is 5~15%.
7. ferment facial mask essence according to claim 6, it is characterised in that the mass percent of the skin care enzyme liquid
For 10%.
8. ferment facial mask essence according to claim 6, it is characterised in that the ferment facial mask essence is according to weight
Percentages, including following composition：Water 70~90%；Skin care enzyme liquid 5~15%；Glycerine 2~4%；Ginseng root extract 1~
3%；Butanediol 0.5~3%；Trehalose 0.5~3%；Sodium Hyaluronate 0.02~0.2；Panthenol 0.5~1.0%；Tocopherol
0.1~0.5%, allantoin 0.2~0.5%, ursin 0.2~1%；Cremophor RH40 0.3~1.5%；Carbomer
0.1~0.3%；Glycerine polymethacrylates 0.1~0.5%；Sodium hydroxide 0.01~0.05%；Methylisothiazolinone
0.005~0.01%；Iodine propilolic alcohol butyl mephenesin Carbamate 0.005~0.02%.
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