CN104995309B - 多重核酸检测方法 - Google Patents

多重核酸检测方法 Download PDF

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CN104995309B
CN104995309B CN201380072327.3A CN201380072327A CN104995309B CN 104995309 B CN104995309 B CN 104995309B CN 201380072327 A CN201380072327 A CN 201380072327A CN 104995309 B CN104995309 B CN 104995309B
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probe
different
nucleic acids
target nucleic
target
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CN104995309A (zh
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E·奥利瓦瑞斯
J·索伦森
T·兰德斯
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Invitae Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
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  • Biotechnology (AREA)
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  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201380072327.3A 2012-12-07 2013-12-06 多重核酸检测方法 Active CN104995309B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261734838P 2012-12-07 2012-12-07
US61/734,838 2012-12-07
PCT/US2013/073745 WO2014089536A1 (en) 2012-12-07 2013-12-06 Multiplex nucleic acid detection methods

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CN104995309A CN104995309A (zh) 2015-10-21
CN104995309B true CN104995309B (zh) 2020-12-08

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US (1) US9624533B2 (enExample)
EP (1) EP2929044B1 (enExample)
JP (1) JP6484177B2 (enExample)
CN (1) CN104995309B (enExample)
BR (1) BR112015013234B1 (enExample)
CA (1) CA2894381C (enExample)
CL (1) CL2015001568A1 (enExample)
HK (1) HK1216653A1 (enExample)
IL (1) IL239258B (enExample)
WO (1) WO2014089536A1 (enExample)

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EP3968023B1 (en) 2013-08-19 2025-10-08 Invitae Corporation Assays for single molecule detection and use thereof
WO2016070164A1 (en) * 2014-11-01 2016-05-06 Singular Bio, Inc. Assays for single molecule detection and use thereof
CN113528623A (zh) * 2015-02-18 2021-10-22 卓异生物公司 用于单分子检测的测定及其应用
CN107083427B (zh) * 2016-04-01 2021-07-30 广州市基准医疗有限责任公司 Dna连接酶介导的dna扩增技术
US11702702B2 (en) 2016-04-15 2023-07-18 Predicine, Inc. Systems and methods for detecting genetic alterations
US11174503B2 (en) 2016-09-21 2021-11-16 Predicine, Inc. Systems and methods for combined detection of genetic alterations
CA3048420C (en) * 2016-12-09 2025-05-20 Boreal Genomics Inc Linked ligation
WO2018127408A1 (en) * 2017-01-05 2018-07-12 Tervisetehnoloogiate Arenduskeskus As Quantifying dna sequences
US20180237853A1 (en) * 2017-02-19 2018-08-23 Yan Wang Methods, Compositions and Kits for Detection of Mutant Variants of Target Genes
CN107177693B (zh) * 2017-07-19 2020-11-06 中山大学 一种多倍pcr扩增方法
WO2019046768A1 (en) * 2017-08-31 2019-03-07 William Marsh Rice University SYMBOLIC SEQUENCING OF DNA AND RNA BY SEQUENCE CODING
CN110656159B (zh) * 2018-06-28 2024-01-09 深圳华大生命科学研究院 一种拷贝数变异的检测方法
EP3830828A4 (en) * 2018-07-27 2022-05-04 Myriad Women's Health, Inc. METHODS FOR DETECTING GENETIC VARIATIONS IN HIGHLY HOMOLOGOUS SEQUENCES BY INDEPENDENT ALIGNMENT AND PAIRING OF SEQUENCE READS
CN109402232A (zh) * 2018-10-23 2019-03-01 中国农业大学 一种用于检测的组合物及检测方法
US20220290242A1 (en) * 2018-11-05 2022-09-15 Centre Henri Becquerel Method for diagnosing a cancer and associated kit

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101395280A (zh) * 2006-03-01 2009-03-25 凯津公司 基于测序的高通量SNPs连接检测技术
WO2011091106A2 (en) * 2010-01-22 2011-07-28 The Regents Of The University Of California Compositions and methods for gene signature - based chemical screening
CN102648295A (zh) * 2009-12-07 2012-08-22 伊鲁米那股份有限公司 用于多重基因分型的多样品索引
WO2012158967A1 (en) * 2011-05-17 2012-11-22 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids

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US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5508179A (en) 1994-03-18 1996-04-16 Bio-Rad Laboratories, Inc. Use of deoxyribose nicotinamide adenine dinucleotide to enhance the specificity of NAD+ -dependent ligation reactions
EP2369007B1 (en) * 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
SE0401270D0 (sv) 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel
US8852864B2 (en) * 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
CA2757300C (en) * 2009-04-01 2018-01-09 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (clpa)
US20130225420A1 (en) * 2010-08-02 2013-08-29 The Regents Of The University Of California Molecular subtyping of oral squamous cell carcinoma to distinguish a subtype that is unlikely to metastasize

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101395280A (zh) * 2006-03-01 2009-03-25 凯津公司 基于测序的高通量SNPs连接检测技术
CN102648295A (zh) * 2009-12-07 2012-08-22 伊鲁米那股份有限公司 用于多重基因分型的多样品索引
WO2011091106A2 (en) * 2010-01-22 2011-07-28 The Regents Of The University Of California Compositions and methods for gene signature - based chemical screening
WO2012158967A1 (en) * 2011-05-17 2012-11-22 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids

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JP6484177B2 (ja) 2019-03-13
CA2894381A1 (en) 2014-06-12
US9624533B2 (en) 2017-04-18
CL2015001568A1 (es) 2016-08-26
BR112015013234A2 (pt) 2018-02-06
EP2929044A4 (en) 2016-07-13
CN104995309A (zh) 2015-10-21
US20140235470A1 (en) 2014-08-21
JP2016500257A (ja) 2016-01-12
EP2929044A1 (en) 2015-10-14
IL239258A0 (en) 2015-07-30
EP2929044B1 (en) 2019-02-06
WO2014089536A1 (en) 2014-06-12
HK1216653A1 (zh) 2016-11-25
BR112015013234B1 (pt) 2021-12-21
CA2894381C (en) 2021-01-12
IL239258B (en) 2019-12-31

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