CN104926421A - High-rutin efficient culture medium for coprinus comatus prepared from tartary buckwheat straws and preparation method - Google Patents
High-rutin efficient culture medium for coprinus comatus prepared from tartary buckwheat straws and preparation method Download PDFInfo
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- CN104926421A CN104926421A CN201510385419.8A CN201510385419A CN104926421A CN 104926421 A CN104926421 A CN 104926421A CN 201510385419 A CN201510385419 A CN 201510385419A CN 104926421 A CN104926421 A CN 104926421A
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Abstract
The invention discloses a high-rutin efficient culture medium for coprinus comatus prepared from tartary buckwheat straws. The culture medium is characterized by comprising the following components in parts by weight: 10-20 parts of sludge of a monosodium glutamate plant, 20-30 parts of soybean skin, 10-20 parts of bean pulp, 1-2 parts of aspergillus oryzae, 1.2-1.6 parts of trichoderma reesei, 50-100 parts of tartary buckwheat straws, 1-2 parts of white rot fungi, 1.1-1.3 parts of bacillus subtilis, 0.9-1.3 parts of geobacillus stearothermophilus, 1.5-2.5 parts of organic modified chitosan fiber, 1-2 parts of superfine carbon powder, 1.3-1.5 parts of calcium pantothenate, 2-4 parts of glucose, 1-2 parts of ammonium sulfate and 0.2-0.4 part of cellulose. According to the culture medium disclosed by the invention, the insoluble organic matter high-rutin tartary buckwheat straws are degraded and the soybean skin, the bean pulp and the leaf meal are fermented respectively by adopting organic modified chitosan fiber loaded activated biochemical invertase and organic modified chitosan fiber loaded activated zymophyte, the degradation and fermentation speed is effectively adjusted, the formation of an intermediate product is promoted, and finally, the reaction of the intermediate product is promoted through high-temperature stirring reaction to form high-activity biochemical humic acid, so that absorption and metabolism of auricularia auricular are facilitated, and efficient cultivation of coprinus comatus is realized.
Description
Technical field
The invention belongs to breed of edible fungus field, be specifically related to a kind of Coprinus comatus high rutin efficient culture medium and the preparation method that adopt bitter buckwheat bar to prepare.
Background technology
Edible mushrooms has abundant nutrition and good nourishing function because of it, enjoys the parent of people to look at.In recent years, edible mushrooms market scale constantly expands, and in order to meet the need of market, China's Edible Fungi is just towards the future development of industrialization, mass-producing and batch production.The edible fungus culturing fertilizer of green high-efficient has become an urgent demand of edible mushrooms development.Meanwhile, also to there is raw material availability low for Edible Fungi.Output needs the problem improved further.
Biochemical humic acid is by chemistry with microorganism fermentation process makes is a kind of organism by agricultural crop straw, wood chip etc., except containing except active xanthohumic acid, amino acid also containing some amount and abundant carboxyl, hydroxyl, phenolic hydroxyl group, quinone hydroxyl isoreactivity group, compared with mineral humic acid, have the following advantages: 1, molecular weight is little, seepage force is strong, more easily absorbed by crops and utilization; 2, active group rich content, physiologically active and chemically reactive stronger; 3, composition variation, active high, effect is more excellent.
Summary of the invention
The present invention is directed to the artificial culture demand of edible mushrooms, propose a kind of Coprinus comatus high rutin efficient culture medium and the preparation method that adopt bitter buckwheat bar to prepare.
The technical solution used in the present invention is as follows:
One with kind of the high rutin efficient culture medium of Coprinus comatus adopting bitter buckwheat bar to prepare, it is characterized in that, comprise following parts by weight of component: Gourmet Powder Factory mud 10-20, Testa Glycines 20-30, bean cake powder 10-20, aspergillus oryzae 1-2, Trichodermareesei 1.2-1.6, bitter buckwheat bar 50-100, whiterot fungi 1-2, subtilis 1.1-1.3, Geobacillus stearothermophilus 0.9-1.3, organically-modified chitin fiber 1.5-2.5, Ultrafine Silicon Powders 1-2, calcium pantothenate 1.3-1.5, glucose 2-4, ammonium sulfate 1-2, walnut leaf 2-10, apple leaf 4-10, birch leaf 10-20 part, Mierocrystalline cellulose 0.2-0.4, appropriate water.
The high rutin efficient culture medium preparation method of the Coprinus comatus adopting bitter buckwheat bar to prepare, is characterized in that, comprise the following steps:
(1) be the 40-60% of gross weight by the airing of bitter buckwheat bar to moisture content, put into the section that pulverizer is ground into 1-2cm and adopt High Temperature High Pressure decompressor process 2-3min, obtain expansion activated camplex for subsequent use;
(2) the organically-modified chitin fiber of half, Ultrafine Silicon Powders, half glucose and gross weight 1-2 water mix and blend 5-10min is doubly got to even, cooling, add aspergillus oryzae, Trichodermareesei stirs, leave standstill 3-5h, the activation biochemical conversion enzyme obtaining chitin fiber loading is for subsequent use; Get second half organically-modified chitin fiber, glucose and gross weight 1-2 water mix and blend 5-10min doubly to even, add whiterot fungi, subtilis, Geobacillus stearothermophilus stir, leave standstill 3-5h, the activation zymophyte obtaining the loading of organically-modified chitin fiber is for subsequent use;
(3) the activation biochemical conversion enzyme mixing of expansion activated camplex, the loading of organically-modified chitin fiber is got, and add gross weight 3-5 water doubly, airtight stirring 20-30min is to even, airtight placement 2-3d, add cellulase afterwards and stir enzymolysis 2-30min at 40-50 DEG C, bitter buckwheat bar degraded mixture must be activated for subsequent use;
(4) walnut leaf, apple leaf, birch leaf mixing is got, pulverize, obtain the activation zymophyte mixing of Testa Glycines, bean cake powder, the loading of organically-modified chitin fiber, and after adding gross weight 3-5 water mixing doubly, sealing and fermenting 10-12d, pumps into the bitter buckwheat bar degraded mixture of activation afterwards and stirs, leave standstill 2-3d, 80-90 DEG C of stirring reaction 30-50min in closed environment, cooling, obtains biochemical humic acid mixture for subsequent use;
(5) get calcium pantothenate, ammonium sulfate and gross weight 2-3 water doubly to mix to evenly, obtain nutritive medium for subsequent use;
(6) get Gourmet Powder Factory's mud, biochemical humic acid mixture, nutritive medium Homogeneous phase mixing liquid mix and blend, and adjust the 50-60% that moisture content is gross weight, obtain culture material for subsequent use;
(7) be divided in polyethylene plastic bag by culture material, put into the steamer of band high-pressure valve and cooling valve afterwards, first vigorous fire is warming up to 100 DEG C, sterilizing 1-2h, being cooled to rapidly 0 DEG C by cooling valve, is warming up to 100 DEG C at secondary vigorous fire, sterilizing 30-50min, is cooled to room temperature, to obtain final product.
Beneficial effect of the present invention is embodied in:
The activation biochemical conversion enzyme that the present invention adopts organically-modified chitin fiber to load and the activation zymophyte that organically-modified chitin fiber loads carry out the bitter degraded of buckwheat bar of organic object height rutin hard to tolerate and the fermentation of skin of beancurd, dregs of beans and leaf powder respectively, effectively have adjusted degraded and fermenting speed, facilitate the formation of intermediate product, finally reacted by high-temperature stirring, facilitate the reaction of intermediate product, form highly active biochemical humic acid, be convenient to absorption and the metabolism of black fungus, realize the Efficient Cultivation of Coprinus comatus.
Embodiment
Embodiment
Described in the present embodiment one is with kind of the high rutin efficient culture medium of Coprinus comatus adopting bitter buckwheat bar to prepare, it is characterized in that, comprise following parts by weight of component: Gourmet Powder Factory's mud 15, Testa Glycines 25, bean cake powder 15, aspergillus oryzae 1.5, Trichodermareesei 1.4, bitter buckwheat bar 75, whiterot fungi 1.5, subtilis 1.2, Geobacillus stearothermophilus 1.1, organically-modified chitin fiber 2, Ultrafine Silicon Powders 1.5, calcium pantothenate 1.4, glucose 3, ammonium sulfate 1.5, walnut leaf 6, apple leaf 7, birch leaf 15 parts, Mierocrystalline cellulose 0.3, appropriate water.
The high rutin efficient culture medium preparation method of Coprinus comatus prepared by the bitter buckwheat bar of the employing described in the present embodiment, is characterized in that, comprise the following steps:
(1) be the 40-60% of gross weight by the airing of bitter buckwheat bar to moisture content, put into the section that pulverizer is ground into 1-2cm and adopt High Temperature High Pressure decompressor process 2-3min, obtain expansion activated camplex for subsequent use;
(2) the organically-modified chitin fiber of half, Ultrafine Silicon Powders, half glucose and gross weight 1-2 water mix and blend 5-10min is doubly got to even, cooling, add aspergillus oryzae, Trichodermareesei stirs, leave standstill 3-5h, the activation biochemical conversion enzyme obtaining chitin fiber loading is for subsequent use; Get second half organically-modified chitin fiber, glucose and gross weight 1-2 water mix and blend 5-10min doubly to even, add whiterot fungi, subtilis, Geobacillus stearothermophilus stir, leave standstill 3-5h, the activation zymophyte obtaining the loading of organically-modified chitin fiber is for subsequent use;
(3) the activation biochemical conversion enzyme mixing of expansion activated camplex, the loading of organically-modified chitin fiber is got, and add gross weight 3-5 water doubly, airtight stirring 20-30min is to even, airtight placement 2-3d, add cellulase afterwards and stir enzymolysis 2-30min at 40-50 DEG C, bitter buckwheat bar degraded mixture must be activated for subsequent use;
(4) walnut leaf, apple leaf, birch leaf mixing is got, pulverize, obtain the activation zymophyte mixing of Testa Glycines, bean cake powder, the loading of organically-modified chitin fiber, and after adding gross weight 3-5 water mixing doubly, sealing and fermenting 10-12d, pumps into the bitter buckwheat bar degraded mixture of activation afterwards and stirs, leave standstill 2-3d, 80-90 DEG C of stirring reaction 30-50min in closed environment, cooling, obtains biochemical humic acid mixture for subsequent use;
(5) get calcium pantothenate, ammonium sulfate and gross weight 2-3 water doubly to mix to evenly, obtain nutritive medium for subsequent use;
(6) get Gourmet Powder Factory's mud, biochemical humic acid mixture, nutritive medium Homogeneous phase mixing liquid mix and blend, and adjust the 50-60% that moisture content is gross weight, obtain culture material for subsequent use;
(7) be divided in polyethylene plastic bag by culture material, put into the steamer of band high-pressure valve and cooling valve afterwards, first vigorous fire is warming up to 100 DEG C, sterilizing 1-2h, being cooled to rapidly 0 DEG C by cooling valve, is warming up to 100 DEG C at secondary vigorous fire, sterilizing 30-50min, is cooled to room temperature, to obtain final product.
Claims (2)
1. the high rutin efficient culture medium of Coprinus comatus adopting bitter buckwheat bar to prepare, it is characterized in that, comprise following parts by weight of component: Gourmet Powder Factory mud 10-20, Testa Glycines 20-30, bean cake powder 10-20, aspergillus oryzae 1-2, Trichodermareesei 1.2-1.6, bitter buckwheat bar 50-100, whiterot fungi 1-2, subtilis 1.1-1.3, Geobacillus stearothermophilus 0.9-1.3, organically-modified chitin fiber 1.5-2.5, Ultrafine Silicon Powders 1-2, calcium pantothenate 1.3-1.5, glucose 2-4, ammonium sulfate 1-2, walnut leaf 2-10, apple leaf 4-10, birch leaf 10-20 part, Mierocrystalline cellulose 0.2-0.4, appropriate water.
2. the high rutin efficient culture medium preparation method of the Coprinus comatus adopting bitter buckwheat bar to prepare, is characterized in that, comprise the following steps:
(1) be the 40-60% of gross weight by the airing of bitter buckwheat bar to moisture content, put into the section that pulverizer is ground into 1-2cm and adopt High Temperature High Pressure decompressor process 2-3min, obtain expansion activated camplex for subsequent use;
(2) the organically-modified chitin fiber of half, Ultrafine Silicon Powders, half glucose and gross weight 1-2 water mix and blend 5-10min is doubly got to even, cooling, add aspergillus oryzae, Trichodermareesei stirs, leave standstill 3-5h, the activation biochemical conversion enzyme obtaining chitin fiber loading is for subsequent use; Get second half organically-modified chitin fiber, glucose and gross weight 1-2 water mix and blend 5-10min doubly to even, add whiterot fungi, subtilis, Geobacillus stearothermophilus stir, leave standstill 3-5h, the activation zymophyte obtaining the loading of organically-modified chitin fiber is for subsequent use;
(3) the activation biochemical conversion enzyme mixing of expansion activated camplex, the loading of organically-modified chitin fiber is got, and add gross weight 3-5 water doubly, airtight stirring 20-30min is to even, airtight placement 2-3d, add cellulase afterwards and stir enzymolysis 2-30min at 40-50 DEG C, bitter buckwheat bar degraded mixture must be activated for subsequent use;
(4) walnut leaf, apple leaf, birch leaf mixing is got, pulverize, obtain the activation zymophyte mixing of Testa Glycines, bean cake powder, the loading of organically-modified chitin fiber, and after adding gross weight 3-5 water mixing doubly, sealing and fermenting 10-12d, pumps into the bitter buckwheat bar degraded mixture of activation afterwards and stirs, leave standstill 2-3d, 80-90 DEG C of stirring reaction 30-50min in closed environment, cooling, obtains biochemical humic acid mixture for subsequent use;
(5) get calcium pantothenate, ammonium sulfate and gross weight 2-3 water doubly to mix to evenly, obtain nutritive medium for subsequent use;
(6) get Gourmet Powder Factory's mud, biochemical humic acid mixture, nutritive medium Homogeneous phase mixing liquid mix and blend, and adjust the 50-60% that moisture content is gross weight, obtain culture material for subsequent use;
(7) be divided in polyethylene plastic bag by culture material, put into the steamer of band high-pressure valve and cooling valve afterwards, first vigorous fire is warming up to 100 DEG C, sterilizing 1-2h, being cooled to rapidly 0 DEG C by cooling valve, is warming up to 100 DEG C at secondary vigorous fire, sterilizing 30-50min, is cooled to room temperature, to obtain final product.
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Citations (4)
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| CN102850098A (en) * | 2012-09-29 | 2013-01-02 | 南开大学 | Production method of efficient odorless organic fertilizer |
| CN104109004A (en) * | 2014-07-22 | 2014-10-22 | 肥东县丰宝种养殖有限责任公司 | Lentinus edodes culture medium containing broadleaf sawdust and preparation method of lentinus edodes culture medium |
| CN104230541A (en) * | 2014-08-28 | 2014-12-24 | 岳西县利泰农业发展有限责任公司 | Black fungus culture medium prepared from sorghum straw and preparation method thereof |
| CN104232536A (en) * | 2014-09-03 | 2014-12-24 | 稼禾生物股份有限公司 | Preparation method of fermentation bacterium agent |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102850098A (en) * | 2012-09-29 | 2013-01-02 | 南开大学 | Production method of efficient odorless organic fertilizer |
| CN104109004A (en) * | 2014-07-22 | 2014-10-22 | 肥东县丰宝种养殖有限责任公司 | Lentinus edodes culture medium containing broadleaf sawdust and preparation method of lentinus edodes culture medium |
| CN104230541A (en) * | 2014-08-28 | 2014-12-24 | 岳西县利泰农业发展有限责任公司 | Black fungus culture medium prepared from sorghum straw and preparation method thereof |
| CN104232536A (en) * | 2014-09-03 | 2014-12-24 | 稼禾生物股份有限公司 | Preparation method of fermentation bacterium agent |
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Application publication date: 20150923 |