CN104906176A - Method for drying medicinal material radix astragali - Google Patents
Method for drying medicinal material radix astragali Download PDFInfo
- Publication number
- CN104906176A CN104906176A CN201510360652.0A CN201510360652A CN104906176A CN 104906176 A CN104906176 A CN 104906176A CN 201510360652 A CN201510360652 A CN 201510360652A CN 104906176 A CN104906176 A CN 104906176A
- Authority
- CN
- China
- Prior art keywords
- group
- radix astragali
- drying
- cloudy
- microwave
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Dairy Products (AREA)
Abstract
The invention discloses a processing method for drying a medicinal material radix astragali by microwave and infrared combination, aiming at the technical problems in the prior art that not only is the quality of medicinal materials affected but also various harmful substances can be brought in when traditional Chinese medicines are fumigated with sulfur. The method comprises the following steps: impurity removal, grading according to the size, smoothing straight, microwave drying, chilling, infrared drying, chilling, tidying, binding and tying and subpackaging. The method can achieve the aim of drying the medicinal material radix astragali faster. Compared with traditional methods, the method has the beneficial effect that the moisture content of the medicinal material can fast reach the limit stipulated in Chinese Pharmacopoeia on the basis of not reducing the main ingredients of the medicinal material. Compared with a sulfur fumigation method, the method has the beneficial effect that the harmful substances are prevented from being brought in. Meanwhile, the method has the beneficial effects that the medicinal material radix astragali has obvious effects on improvement of immunities of mice; mildewing, damages caused by worms and other phenomena are not easy to happen in the storage process; the stability of the quality of the medicinal material radix astragali is ensured; radix astragali processing is more normalized through tidying and subpackaging, thus improving the quality controllability of the medicinal material radix astragali.
Description
Technical field
The invention belongs to Chinese medicine manufacture field, be specially the method for a kind of Milkvetch Root drying processing.
Background technology
The Radix Astragali is the dry root of leguminous plant Radix Astagali or Radix Astragali, and the Radix Astragali is as Chinese medicine, and application is comparatively extensive, and large usage quantity in tcm clinical practice, output is also higher.The Radix Astragali originates from the ground such as Gansu, Shaanxi, Shanxi, the Inner Mongol, Anhui and Heilungkiang.Its traditional diamond-making technique is for drying or drying, and this method length consuming time, is subject to weather effect, and not easily preserves; For make Milkvetch Root more attractive in appearance, more easily store, basic unit processing staff commonly uses sulfur fumigation Milkvetch Root, but sulfur fumigation Chinese crude drug not only affects the quality of medical material own also can introduce multiple harmful substances, causes serious harm to health.Therefore, seek the Radix Astragali drying and processing method of new specification, make it under the prerequisite not affecting Radix Astragali quality, extend period of storage as far as possible, development Radix Astragali industry is had great importance.
Summary of the invention
Not only affect for sulfur fumigation Chinese crude drug in prior art the technical problem that the quality of medical material own also can introduce multiple harmful substances, the invention provides the Milkvetch Root drying and processing method that a kind of specification is practical, be convenient to popularization, concrete technical scheme is as follows:
By the fresh Radix Astragali after gathering, removing impurity and residual stem, reed head, after diameter 3cm, 2cm, 1cm and following Three Estate stepping, be placed in microwave drying oven and be dried to medical material moisture 19% ~ 28% time, taking-up is put into infrared drying oven and is dried to medical material moisture further below 10%, take out, cool, to obtain final product.
As a further improvement on the present invention, the drying condition of described microwave drying oven is 57 ~ 65W, dry 20 ~ 30 min; The drying condition of described infrared drying oven is 1000 ~ 3000 W, dry 15 ~ 20 min.
Further improve as of the present invention, the drying condition of described microwave drying oven is 61W, dry 20 min, and the drying condition of described infrared drying oven is 1000W, dry 20min.
beneficial effect
1 compares with traditional method, and hot blast provided by the invention-microwave combination technology makes its water content reach " Chinese Pharmacopoeia " prescribed limit fast on the basis not reducing medical material main component; Compare with sulfur fumigation method, microwave-infrared multiple techniques avoids the introducing of harmful substance, it also avoid the harm that some processing staff uses sulfur fumigation to bring in dry run simultaneously.
2, in the Radix Astragali sample that microwave provided by the invention-infrared multiple techniques machining obtains, the overall content of two kinds of principle active component astragalosides, calycosin glucoside is better than other processing groups.
3, drug efficacy study experiment shows, compared to other processing methods, the Milkvetch Root of microwave-infrared multiple techniques processing has most remarkable result in raising mouse immunity.
4, the Milkvetch Root after microwave provided by the invention-infrared multiple techniques processing simultaneously; not easily to go mouldy in storage process the phenomenon such as to damage by worms; experiment confirms that the antibacterial of Radix Astragali storage after 0,6,12 month, mycete, yeast, escherichia coli, coliform, the quantity such as demodicid mite of living all are less than other processing groups; so just can guarantee Milkvetch Root steady quality; arrangement, subpackage make Radix Astragali processing more standardize, and improve the quality controllability of Milkvetch Root.
Accompanying drawing explanation
Fig. 1 Milkvetch Root sample compares before and after damaging by worms;
Wherein, A: before damaging by worms; B: after damaging by worms.
Detailed description of the invention
embodiment 1milkvetch Root preprocess method (remove impurity, stepping)
The fresh Radix Astragali after gathering, removing impurity and residual stem, reed head, according to diameter 3 cm, 2 cm, 1 cm and following Three Estate stepping.
the traditional dry in the sun group of embodiment 2: under the Milkvetch Root be disposed by embodiment 1 is laid in sunlight respectively according to different brackets, dry in the sun is dried or dries in the shade in indoor airing, records weight change constantly, until medical material moisture is below 10%.
embodiment 3 hot blast processing group: the Milkvetch Root be disposed by embodiment 1 is placed in electric heating constant-temperature blowing drying box respectively according to different brackets, moisture is dried to about 46% time respectively at 60 DEG C, 70 DEG C, 80 DEG C, take out, smooth out with the fingers straight after slightly cool, continue to be dried to moisture at the same temperature below 10%, take out, cool, to obtain final product.Hot blast 60 DEG C of processing groups, hot blast 70 DEG C of processing groups and hot blast 80 DEG C of processing groups are divided into according to baking temperature difference.
embodiment 4 microwave processing group: the Milkvetch Root be disposed by embodiment 1 is placed in microwave drier respectively according to different brackets and is machined to moisture below 10% under 380V, 80mA condition.
the infrared processing group of embodiment 5: the Milkvetch Root be disposed by embodiment 1 is placed in 1000W in infrared heating device respectively according to different brackets and is machined to moisture below 10%.
embodiment 6 hot wind and microwave coupling group: the Milkvetch Root be disposed by embodiment 1 is placed in electric heating constant-temperature blowing drying box respectively according to different brackets, 60 DEG C are dried to moisture 46% time, take out, smooth out with the fingers straight after slightly cool, when continuing to be dried to moisture 28%, put into microwave drying oven, dry 10 min under 57 ~ 65W condition, are dried to medical material moisture below 10%, take out, cool, to obtain final product.
embodiment 7 infrared hot-air coupling group: the Milkvetch Root be disposed by embodiment 1 is placed in electric heating constant-temperature blowing drying box respectively according to different brackets, 60 DEG C are dried to moisture about 46% time, take out, smooth out with the fingers straight after slightly cool, continue to be dried to moisture 28% time, be placed in the inherent 1000W of infrared heating device and be machined to moisture below 10%.
embodiment 8 microwave-infrared coupling group: the Milkvetch Root be disposed by embodiment 1 is placed in microwave drier dry 20min under 380V, 80mA condition respectively according to different brackets, takes out, is placed in infrared drying oven, 1000W drying about 20 min, is dried to medical material moisture below 10%, takes out, cool, to obtain final product.
the infrared coupling group of embodiment 9 hot wind and microwave:the Milkvetch Root be disposed by embodiment 1 is placed in electric heating constant-temperature blowing drying box respectively according to different brackets, 60 DEG C are dried to moisture when about 46 %, take out, and smooth out with the fingers straight after slightly cool, be placed in microwave drier dry 20 min under 380V, 80mA condition again, take out, be placed in infrared drying oven, 1000W drying about 20min, be dried to medical material moisture below 10%, take out, cool, to obtain final product.
embodiment 10 stove drying group: the Milkvetch Root be disposed by embodiment 1 shine respectively to sixty percent dry, most probably dry, hundred per cent is done time, clean Milkvetch Root rapidly, after surface moisture dries, being placed in self-control wooden case, is that 30g/kg medical material is fumigated Milkvetch Root with sulfur consumption, after one night, open wooden case, treat that sulfur cigarette disperses, take out medical material, shine again and do to hundred per cent, to obtain final product.Sixty percent dry stove drying group (moisture 46%), most probably dry stove drying group (moisture 28 %) and hundred per cent is divided into do stove drying group (moisture 10%) according to the difference of moisture in medical material during sulfur fumigation Milkvetch Root.
embodiment 11 sulfur many times of groups: when the Milkvetch Root be disposed by embodiment 1 shines respectively and does to hundred per cent, clean Milkvetch Root rapidly, after surface moisture dries, being placed in self-control wooden case, is that 30g/kg, 60g/kg, 90g/kg medical material is fumigated Milkvetch Root with sulfur consumption, after one night, open wooden case, treat that sulfur cigarette disperses, take out medical material, shine again and do to hundred per cent, to obtain final product.Different according to the consumption of sulfur during sulfur fumigation Milkvetch Root, be divided into qdx sulfur fumigation group and triplication sulfur fumigation group.
embodiment 12 stove drying repeatedly group: when the Milkvetch Root be disposed by embodiment 1 shines respectively and does to hundred per cent, clean Milkvetch Root rapidly, after surface moisture dries, being placed in self-control wooden case, is that 30g/kg medical material was fumigated Milkvetch Root, after the night with sulfur consumption, open wooden case, treat that sulfur cigarette disperses, take out medical material, then shine to hundred per cent dry, every two days later, again Milkvetch Root is smoked once in the same way again, then shine to hundred per cent dry, obtain stove drying twice sample.Sample after stove drying twice, every two days later, processes Milkvetch Root in the same way, obtains stove drying three samples.According to the difference of fumigating number of times during sulfur fumigation Milkvetch Root, be divided into stove drying twice group and stove drying three groups.
embodiment 13: the comparative test of active constituent content in the Radix Astragali different processing group sample
This experiment adopts contained two main component astragalosides of States Pharmacopoeia specifications detection and the content of calycosin glucoside in the high performance liquid chromatography comparative measurements Radix Astragali different processing group sample, its content assaying method measures according to method under version " Chinese Pharmacopoeia " (one) Radix Astragali assay item in 2010, the results are shown in Table 1.
The content (n=3) of two-component in each processing group of table 1 Radix Astragali
| Numbering | Grouping | Sample preparation methods | Astragaloside %(is in dry product) | Calycosin glucoside %(is in dry product) |
| 1 | Tradition dries group | Embodiment 2 | 0.150 | 0.0830 |
| 2 | Hot blast 60 DEG C of processing groups | Embodiment 3 | 0.143 | 0.0797 |
| 3 | Hot blast 70 DEG C of processing groups | Embodiment 3 | 0.143 | 0.0753 |
| 4 | Hot blast 80 DEG C of processing groups | Embodiment 3 | 0.129 | 0.0683 |
| 5 | Microwave processing group | Embodiment 4 | 0.177 | 0.0796 |
| 6 | Infrared processing group | Embodiment 5 | 0.144 | 0.0651 |
| 7 | Hot wind and microwave coupling group | Embodiment 6 | 0.214 | 0.0694 |
| 8 | Infrared hot-air coupling group | Embodiment 7 | 0.157 | 0.0517 |
| 9 | Microwave-infrared coupling group | Embodiment 8 | 0.183 | 0.0734 |
| 10 | The infrared coupling group of hot wind and microwave | Embodiment 9 | 0.101 | 0.0733 |
| 11 | Sixty percent dry stove drying group | Embodiment 10 | 0.126 | 0.0801 |
| 12 | Most probably dry stove drying group | Embodiment 10 | 0.115 | 0.0687 |
| 13 | Hundred per cent does stove drying group | Embodiment 10 | 0.135 | 0.0793 |
| 14 | Qdx sulfur fumigation group | Embodiment 11 | 0.145 | 0.0766 |
| 15 | Triplication sulfur fumigation group | Embodiment 11 | 0.190 | 0.0756 |
| 16 | Stove drying twice group | Embodiment 12 | 0.159 | 0.0631 |
| 17 | Stove drying three groups | Embodiment 12 | 0.176 | 0.0559 |
Result shows, in the Radix Astragali sample that the hot blast-microwave combination shown in the 7th, 8 group, microwave-infrared coupling machining obtains, the overall content of two kinds of principle active component is better than other groups.Along with the increase of sulfur consumption and increasing of stove drying number of times, Astragaloside content is rising trend, and compared with tradition processing group, may be converted into astragaloside for other compositions under certain stove drying condition.This is relevant with the character of astragaloside; astragaloside is only containing two sugared keys; usual Radix Astragali saponin I or Radix Astragali saponin II takes off-and namely Ac become astragaloside; may due at certain stove drying temperature in stove drying process; by the impact of the change such as moisture and Acidity of Aikalinity in medical material; facilitate this hydrolytic process, and the increase of sulfur consumption and increasing of stove drying number of times play facilitation to this hydrolytic process to a certain extent.The Changing Pattern of calycosin glucoside content is contrary with astragaloside, may be hydrolyzed to factor that astragaloside plays facilitation and also promotes calycosin glucoside and be hydrolyzed to other compositions due to above-mentioned to Radix Astragali saponin I or Radix Astragali saponin II.
embodiment 14in embodiment 2 ~ 10, the Radix Astragali sample of different processing group is on the impact of the low mice carbonic clearance of cyclophosphamide induction of immunity
1 experiment material
1.1 experimental apparatus: TD5102 electronic balance (Tianjin Mantidis instrument plant), T6 new century UV detector (Beijing Puxi General Instrument Co., Ltd), FA 1104 type electronic analytical balance (ten thousand/, Shanghai Precision Scientific Apparatus Co., Ltd), gastric perfusion needle, 1ml syringe, capillary tube, emery wheel, surface plate, 10ml centrifuge tube, measuring bottle, liquid-transfering gun, cuvette, eye scissors, dissecting scissors, filter paper.
1.2 experiment reagents and medicine: levamisole (Shandong Kernel and Hall Pharmaceutical Industry Co., Ltd., lot number 131202), cyclophosphamide (Tonghua Maoxiang Pharmaceutical Co., Ltd., lot number 130603), india ink (Waldeck GmbH & Co.KG, lot number 391622), 0.9% sodium chloride injection (Beijing company limited of Double-Crane Pharmaceutical Co., Ltd), heparin, sodium carbonate.
1.3 laboratory animals: cleaning grade ICR mice, body weight 20 ± 2g, is provided by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center, and laboratory animal production licence number: SCXK(revives) 2008-0010.
The preparation of 1.4 medicinal liquids
Take the Milkvetch Root after by processing method process in embodiment 2 ~ 10 (being cut into decoction pieces size) 50.0g respectively, soak 0.5 hour post-heating and boil 1 hour, filter, residue adds water and boils 1 hour again, merge twice filtrate, be transferred in 50ml measuring bottle after concentrated, make the medicinal liquid that concentration is 1.0g/ml, at 4 DEG C of refrigerator cold-storages, for subsequent use.
2 experimental techniques
ICR mice 156, male and female half and half, are divided into 13 groups at random, often organize 10, namely blank group, model group, the administration group of 10 different processing methods in positive group and embodiment 2 ~ 12.
Blank group gavage gives 0.1ml/10g mice distilled water, every day 1 time, continuous 10 days.
Model group gives gavage and gives 0.1ml/10g mice distilled water, every day 1 time, continuous 10 days.Within 4th day, start every mouse peritoneal injection 50mg/kg cyclophosphamide (0.1ml/10g mice), every day 1 time, for three days on end, cause Immune Function In Animals inhibition.
Each processing group gavage gives 0.1ml/10g mice, every day 1 time, continuous 10 days.Within 4th day, start every mouse peritoneal injection 50mg/kg cyclophosphamide (0.1ml/10g mice), every day 1 time, for three days on end, cause Immune Function In Animals inhibition.
Positive group gavage gives 30mg/kg levamisole, every day 1 time, continuous 10 days.Within 4th day, start every mouse peritoneal injection 50mg/kg cyclophosphamide (0.1ml/10g), every day 1 time, for three days on end, cause Immune Function In Animals inhibition.
Each group of last administration after 1 hour through mice left eye retroorbital venous clump inject centrifugal after 20% india ink (0. 1ml/10g), inject complete, immediately timing.Get blood from right orbital venous plexus respectively at 5min and the 15min capillary tube of heparin process in advance, in instillation surface plate, draw 40 μ l with liquid-transfering gun immediately, and be added to 4ml 0.l% Na
2cO
3in solution, suction pipe sucks in this liquid, blowout several, fully to wash out the blood of suction pipe wall attachment.Na is added with a blank Mouse Blood
2cO
3solution makes blank, measures absorbance (A) at ultraviolet-uisible spectrophotometer 600nm wavelength place.Claim Mouse Weight and be separated liver spleen, blotting organ surface blood stains with filter paper, weigh respectively, by formulae discovery carbonic clearance index K and phagocytic index α: carbonic clearance index K=(LgA
5-LgA
15)/(t
15-t
5), phagocytic index α=× body weight/(liver weight+spleen weight).The results are shown in Table 2.
3 experimental results
Table 2 Radix Astragali sample is on the impact (n=12) of the low mice carbonic clearance of cyclophosphamide induction of immunity
| Numbering | Group | Preparation method | Carbonic clearance index K | Phagocytic index α |
| 1 | Blank group | -- | 0.058±0.003 | 6.52±0.41 |
| 2 | Model group | -- | 0.044±0.005** | 5.44±0.48** |
| 3 | Dry group | Embodiment 2 | 0.056±0.006 ## | 6.91±0.34 ## |
| 4 | To dry in the shade group | Embodiment 2 | 0.054±0.006 # | 6.18±0.39 # |
| 5 | Hot blast group | Embodiment 3 | 0.058±0.007 ## | 6.51±0.58 ## |
| 6 | Microwave group | Embodiment 4 | 0.055±0.007 ## | 6.84±0.50 ## |
| 7 | Infrared group | Embodiment 5 | 0.054±0.008 ## | 6.57±0.66 ## |
| 8 | Hot blast+microwave group | Embodiment 6 | 0.064±0.007 ## | 7.06±0.48 ## |
| 9 | Hot blast+infrared group | Embodiment 7 | 0.057±0.007 ## | 6.29±0.89 # |
| 10 | Microwave+infrared group | Embodiment 8 | 0.058±0.005 ## | 6.16±0.26 # |
| 11 | Hot blast+microwave+infrared group | Embodiment 9 | 0.053±0.006 # | 6.21±0.58 # |
| 12 | Stove drying group | Embodiment 10 | 0.052±0.007 # | 6.16±0.49 # |
| 13 | Positive group | -- | 0.066±0.008 ## | 7.24±0.68 ## |
Note: compare with blank group, * P < 0.05, * * P < 0.01; Compare with model group,
#p < 0.05,
##p < 0.01.
Result shows, except model group, and each group of there are no significant compared with blank group difference; Each group, compared with model group, all has significant difference.Wherein, hot blast-microwave combination group, hot blast group, hot blast-infrared coupling group effect is more remarkable.
embodiment 15in embodiment 2 ~ 10, the Radix Astragali sample of different processing group is on the impact of DNFB induced mice delayed hypersensitivity
1 experiment material
1.1 experimental apparatus: TD5102 electronic balance (Tianjin Mantidis instrument plant), FA 1104 type electronic analytical balance (ten thousand/, Shanghai Precision Scientific Apparatus Co., Ltd), 8mm card punch, hammer, gastric perfusion needle, 1ml syringe, measuring bottle, liquid-transfering gun, eye scissors, dissecting scissors, filter paper.
1.2 experiment reagents and medicine: levamisole (Shandong Kernel and Hall Pharmaceutical Industry Co., Ltd., lot number 131202), cyclophosphamide (Tonghua Maoxiang Pharmaceutical Co., Ltd., lot number 130603), dinitrofluorobenzene (DNFB), 0.9% sodium chloride injection (Beijing company limited of Double-Crane Pharmaceutical Co., Ltd).
1.3 laboratory animals: cleaning grade ICR mice, body weight 20 ± 2g, is provided by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center, and laboratory animal production licence number: SCXK(revives) 2008-0010.
The preparation of 1.4 medicinal liquids
With in embodiment 14 1.4.
2 experimental techniques
Animal and grouping are with embodiment 14.
Blank group gavage gives 0.1ml/10g mice distilled water, every day 1 time, continuous 9 days.
Model group gives gavage and gives 0.1ml/10g mice distilled water, every day 1 time, continuous 9 days.Within 3rd day, every Mus abdominal part is coated with 1% dinitrofluorobenzene (DNFB) acetone sesame oil solution 50 μ L sensitization (scope 3cm × 3cm), next day with dosage strengthening once, every mouse peritoneal injection 50mg/kg cyclophosphamide (0.1ml/10g mice) simultaneously, every day 1 time, continuous 4 days, cause Immune Function In Animals inhibition.
Each processing group gavage gives 0.1ml/10g mice, every day 1 time, continuous 9 days.Within 3rd day, every Mus abdominal part is coated with 1% dinitrofluorobenzene (DNFB) acetone sesame oil solution 50 μ l sensitization (scope 3cm × 3cm), next day with dosage strengthening once, every mouse peritoneal injection 50mg/kg cyclophosphamide (0.1ml/10g mice) simultaneously, every day 1 time, continuous 4 days, cause Immune Function In Animals inhibition.
Positive group gavage gives 30mg/kg levamisole, every day 1 time, continuous 9 days.Within 3rd day, every Mus abdominal part is coated with 1% dinitrofluorobenzene (DNFB) acetone sesame oil solution 50 μ l sensitization (scope 3cm × 3cm), next day with dosage strengthening once, every mouse peritoneal injection 50mg/kg cyclophosphamide (0.1ml/10g mice) simultaneously, every day 1 time, continuous 4 days, cause Immune Function In Animals inhibition.
1%DNFB solution 10 μ l uniform application, after 5 days, is attacked in mouse right ear two sides by sensitization.Attack latter 24 hours, cervical dislocation puts to death mice, and cut left and right auricular concha, lay round auricle weigh with diameter 8mm card punch, auricle difference in left and right is swelling.
3 experimental results (see table 3)
Table 3 Radix Astragali sample is on the impact (n=12) of DNFB induced mice delayed hypersensitivity
| Group | Process for preparing medicine | Dosage (g/kg) | Ear swelling degree |
| Blank group | -- | - | 1.43±0.16 |
| Model group | -- | - | 3.09±0.26** |
| Dry group | Embodiment 2 | 5 | 1.54±0.22 ## |
| To dry in the shade group | Embodiment 2 | 5 | 1.56±0.13 ## |
| Hot blast group | Embodiment 3 | 5 | 1.48±0.21 ## |
| Microwave group | Embodiment 4 | 5 | 1.52±0.20 ## |
| Infrared group | Embodiment 5 | 5 | 1.53±0.20 ## |
| Hot wind and microwave group | Embodiment 6 | 5 | 1.49±0.15 ## |
| Infrared hot-air group | Embodiment 7 | 5 | 1.53±0.21 ## |
| Microwave-infrared group | Embodiment 8 | 5 | 1.57±0.16 ## |
| Three coupling groups | Embodiment 9 | 5 | 1.64±0.18 ## |
| Stove drying group | Embodiment 10 | 5 | 1.62±0.17 ## |
| Positive group | -- | 5 | 1.40±0.17 ## |
Note: compare with blank group, * P < 0.05, * * P < 0.01; Compare with model group,
#p < 0.05,
##p < 0.01.
Result shows, except model group, and each group of there are no significant compared with blank group difference; Each group, compared with model group, all has significant difference.
embodiment 16in embodiment 2 ~ 10, the Radix Astragali sample of different processing group is on the impact of hypoimmunity mice immune organ weight
1 experiment material
1.1 experimental apparatus: TD5102 electronic balance (Tianjin Mantidis instrument plant), FA 1104 type electronic analytical balance (ten thousand/, Shanghai Precision Scientific Apparatus Co., Ltd), gastric perfusion needle, 1ml syringe, measuring bottle, eye scissors, dissecting scissors, filter paper.
1.2 experiment reagents and medicine: levamisole (Shandong Kernel and Hall Pharmaceutical Industry Co., Ltd., lot number 131202), cyclophosphamide (Tonghua Maoxiang Pharmaceutical Co., Ltd., lot number 130603), 0.9% sodium chloride injection (Beijing company limited of Double-Crane Pharmaceutical Co., Ltd).
1.3 laboratory animals: cleaning grade ICR mice, body weight 20 ± 2g, is provided by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center, and laboratory animal production licence number: SCXK(revives) 2008-0010.
The preparation of 1.4 medicinal liquids
With in embodiment 14 1.4.
2 experimental techniques
Animal and grouping are with embodiment 14.
Blank group gavage gives 0.1ml/10g mice distilled water, every day 1 time, continuous 10 days.
Model group gives gavage and gives 0.1ml/10g mice distilled water, every day 1 time, continuous 10 days.Within 4th day, start every mouse peritoneal injection 50mg/kg cyclophosphamide (0.1ml/10g mice), every day 1 time, for three days on end, cause Immune Function In Animals inhibition.
Each processing group gavage gives 0.1ml/10g mice, every day 1 time, continuous 10 days.Within 4th day, start every mouse peritoneal injection 50mg/kg cyclophosphamide (0.1ml/10g mice), every day 1 time, for three days on end, cause Immune Function In Animals inhibition.
Positive group gavage gives 30mg/kg levamisole, every day 1 time, continuous 10 days.Within 4th day, start every mouse peritoneal injection 50mg/kg cyclophosphamide (0.1ml/10g), every day 1 time, for three days on end, cause Immune Function In Animals inhibition.Last administration claimed mice quality after 1 hour, and solution takes Thymus and spleen takes wet quality, was calculated as follows organ index: organ index=Organ weight (mg)/mice quality (g).
3 experimental results (see table 4)
Table 4 Radix Astragali sample is on the impact (n=12) of hypoimmunity mice immune organ weight
| Group | Process for preparing medicine | Dosage (g/kg) | Index and spleen index | Thymus index |
| Blank group | -- | - | 2.05±0.23 | 0.92±0.11 |
| Model group | -- | - | 1.20±0.17** | 0.62±0.05** |
| Dry group | Embodiment 2 | 5 | 1.83±0.22 ## | 0.84±0.07 ## |
| To dry in the shade group | Embodiment 2 | 5 | 1.78±0.20 ## | 0.83±0.06 ## |
| Hot blast group | Embodiment 3 | 5 | 1.95±0.26 ## | 0.89±0.11 ## |
| Microwave group | Embodiment 4 | 5 | 1.85±0.17 ## | 0.84±0.12 ## |
| Infrared group | Embodiment 5 | 5 | 1.89±0.21 ## | 0.83±0.07 ## |
| Hot wind and microwave group | Embodiment 6 | 5 | 2.00±0.20 ## | 0.85±0.12 ## |
| Infrared hot-air group | Embodiment 7 | 5 | 1.80±0.25 ## | 0.82±0.09 ## |
| Microwave-infrared group | Embodiment 8 | 5 | 1.99±0.18 ## | 0.83±0.11 ## |
| Three coupling groups | Embodiment 9 | 5 | 2.01±0.27 ## | 0.78±0.09* ## |
| Stove drying group | Embodiment 10 | 5 | 1.84±0.25 ## | 0.80±0.05* ## |
| Positive group | -- | 5 | 2.04±0.22 ## | 0.92±0.11 ## |
Note: compare with blank group, * P < 0.05, * * P < 0.01; Compare with model group,
#p < 0.05,
##p < 0.01.
Result shows, except model group, and each group of there are no significant compared with blank group difference; Each group, compared with model group, all has significant difference.Wherein, hot blast group, hot blast-microwave combination group, microwave-infrared coupling group and three coupling group most pronounced effects.
Based on the above results, hot wind and microwave coupling group most pronounced effects, microwave-infrared group also has a remarkable result.
embodiment 17the comparative test of microorganism and acarid number in the different processing group of the Radix Astragali shown in embodiment 2 ~ 12 sample
Under 2010 editions " Chinese Pharmacopoeia " Radix Astragali items, do not specify the inspection item of microorganism, therefore follow the relevant regulations (with reference to annex Ⅹ III C microbial limit test) of preparation limit test of microbe item.Inspection item comprises bacterial population, fungi count, yeast count and Control bacteria examination.Count bacterial population, fungi count, yeast count respectively; Control bacteria examination its with or without.The results are shown in Table 5.
Antibacterial and mycete, yeast number in each processing group of table 5 Radix Astragali
| Sample name | Sample preparation methods | Bacterial population (individual/g) | Mycete, yeast count (individual/g) | Escherichia coli | Coliform | The demodicid mite that lives checks |
| Dry | Embodiment 2 | 9000 | 80 | Cloudy | Cloudy | 2 |
| Hot blast 60 DEG C | Embodiment 3 | 7000 | 60 | Cloudy | Cloudy | Cloudy |
| Hot blast 70 DEG C | Embodiment 3 | 7000 | 60 | Cloudy | Cloudy | Cloudy |
| Hot blast 80 DEG C | Embodiment 3 | 7000 | 60 | Cloudy | Cloudy | Cloudy |
| Microwave | Embodiment 4 | 5000 | 30 | Cloudy | Cloudy | Cloudy |
| Infrared | Embodiment 5 | 6000 | 60 | Cloudy | Cloudy | Cloudy |
| Hot blast+microwave | Embodiment 6 | 5000 | 30 | Cloudy | Cloudy | Cloudy |
| Hot blast+infrared | Embodiment 7 | 6000 | 60 | Cloudy | Cloudy | Cloudy |
| Microwave+infrared | Embodiment 8 | 6000 | 60 | Cloudy | Cloudy | Cloudy |
| Three couplings | Embodiment 9 | 7000 | 60 | Cloudy | Cloudy | Cloudy |
| Sixty percent smokes | Embodiment 10 | 4000 | 10 | Cloudy | Cloudy | Cloudy |
| Most probably smoked | Embodiment 10 | 8000 | 10 | Cloudy | Cloudy | Cloudy |
| Hundred per cent is smoked | Embodiment 10 | 8000 | 10 | Cloudy | Cloudy | Cloudy |
| Qdx stove drying | Embodiment 11 | 8000 | 30 | Cloudy | Cloudy | Cloudy |
| Triplication stove drying | Embodiment 11 | 8000 | 30 | Cloudy | Cloudy | Cloudy |
| 2 single amounts | Embodiment 12 | 8000 | 0 | Cloudy | Cloudy | Cloudy |
| 3 single amounts | Embodiment 12 | 8000 | 30 | Cloudy | Cloudy | Cloudy |
Result shows, the sample sets number of bacteria of hot blast-microwave combination group, hot blast-coupling between two such as infrared coupling group and microwave-infrared coupling group is all less; Except sulfur fumigation group, microwave group and hot wind and microwave group, other respectively mycetes of group, yeast number homogeneous phase with and higher than sulfur fumigation group; Escherichia coli and coliform are feminine gender; The demodicid mite that lives does not detect.Find after Integrated comparative, contained by the sample sets of coupling between two, microbe species and number are all less.
embodiment 18the comparative test of the microorganism after different time and acarid number placed by the yellow Radix Astragali different processing group sample
Under 2010 editions " Chinese Pharmacopoeia " Radix Astragali items, do not specify the inspection item of microorganism, therefore follow the relevant regulations (with reference to annex Ⅹ III C microbial limit test) of preparation limit test of microbe item.Inspection item comprises bacterial population, fungi count, yeast count and Control bacteria examination.Count bacterial population, fungi count, yeast count respectively; Control bacteria examination its with or without.Place and the results are shown in Table 6 in six months, place and the results are shown in Table 7 in 12 months.
The placement of table 6 Radix Astragali each processing group is antibacterial and mycete, yeast number after 6 months
| Sample name | Sample preparation methods | Bacterial population (individual/g) | Mycete, yeast count (individual/g) | Escherichia coli | Coliform | The demodicid mite that lives checks |
| Dry | Embodiment 2 | 15000 | ＞100 | Cloudy | Cloudy | 2 |
| Hot blast 60 DEG C | Embodiment 3 | 10000 | ＞100 | Cloudy | Cloudy | 1 |
| Hot blast 70 DEG C | Embodiment 3 | 10000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Hot blast 80 DEG C | Embodiment 3 | 10000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Microwave | Embodiment 4 | 6000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Infrared | Embodiment 5 | 15000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Hot blast+microwave | Embodiment 6 | 6000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Hot blast+infrared | Embodiment 7 | 7000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Microwave+infrared | Embodiment 8 | 6000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Three couplings | Embodiment 9 | 20000 | ＞100 | Cloudy | Cloudy | 1 |
| Sixty percent smokes | Embodiment 10 | 9000 | 50 | Cloudy | Cloudy | Cloudy |
| Most probably smoked | Embodiment 10 | 10000 | 50 | Cloudy | Cloudy | Cloudy |
| Hundred per cent is smoked | Embodiment 10 | 10000 | 50 | Cloudy | Cloudy | Cloudy |
| Qdx stove drying | Embodiment 11 | 10000 | 40 | Cloudy | Cloudy | 1 |
| Triplication stove drying | Embodiment 11 | 8000 | 50 | Cloudy | Cloudy | Cloudy |
| 2 single amounts | Embodiment 12 | 8000 | 40 | Cloudy | Cloudy | Cloudy |
| 3 single amounts | Embodiment 12 | 10000 | 40 | Cloudy | Cloudy | Cloudy |
The placement of table 7 Radix Astragali each processing group is antibacterial and mycete, yeast number after 12 months
| Sample name | Sample preparation methods | Bacterial population (individual/g) | Mycete, yeast count (individual/g) | Escherichia coli | Coliform | The demodicid mite that lives checks |
| Dry | Embodiment 2 | 20000 | ＞100 | Cloudy | Cloudy | 4 |
| Hot blast 60 DEG C | Embodiment 3 | 10000 | ＞100 | Cloudy | Cloudy | 2 |
| Hot blast 70 DEG C | Embodiment 3 | 10000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Hot blast 80 DEG C | Embodiment 3 | 15000 | ＞100 | Cloudy | Cloudy | 1 |
| Microwave | Embodiment 4 | 10000 | ＞100 | Cloudy | Cloudy | 1 |
| Infrared | Embodiment 5 | 20000 | ＞100 | Cloudy | Cloudy | 2 |
| Hot blast+microwave | Embodiment 6 | 6000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Hot blast+infrared | Embodiment 7 | 8000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Microwave+infrared | Embodiment 8 | 7000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Three couplings | Embodiment 9 | 25000 | ＞100 | Cloudy | Cloudy | 2 |
| Sixty percent smokes | Embodiment 10 | 15000 | ＞100 | Cloudy | Cloudy | 1 |
| Most probably smoked | Embodiment 10 | 20000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Hundred per cent is smoked | Embodiment 10 | 25000 | ＞100 | Cloudy | Cloudy | 2 |
| Qdx stove drying | Embodiment 11 | 15000 | ＞100 | Cloudy | Cloudy | Cloudy |
| Triplication stove drying | Embodiment 11 | 15000 | ＞100 | Cloudy | Cloudy | Cloudy |
| 2 single amounts | Embodiment 12 | 15000 | 80 | Cloudy | Cloudy | Cloudy |
| 3 single amounts | Embodiment 12 | 15000 | 90 | Cloudy | Cloudy | 1 |
Result shows, along with the prolongation of standing time, respectively organizing antibacterial, mycete, yeast and demodicid mite quantity alive all has increase.Wherein the sample sets increasing degree of microwave group, hot blast-microwave combination group, hot blast-coupling between two such as infrared coupling group and microwave-infrared coupling group is less.Find after Integrated comparative, contained by the sample sets of coupling between two, microbe species and number are all less.
In sum, in the Radix Astragali sample that obtains of hot blast-microwave combination, microwave-infrared coupling machining, the overall content of two kinds of principle active component is better than other groups.Microwave-infrared coupling group is Be very effective in raising mouse immunity, and microbe species contained by sample and number are all less.
Note: embodiment Chinese crude drug moisture 46% is defined as sixty percent dry, moisture is 37% be defined as seventy percent dry, and moisture 28% is defined as eighty per cant dry, and moisture 19% is defined as ninety percent dry, and moisture 10% is defined as hundred per cent and does.
Claims (3)
1. the method for a Milkvetch Root drying processing, the fresh Radix Astragali after gathering is removed impurity and residual stem, reed head, according to diameter stepping, then be placed in microwave drying oven and be dried to medical material moisture 19% ~ 28% time, taking-up is put into infrared drying oven and is dried to medical material moisture further below 10%, take out, cool, to obtain final product.
2. the method for Milkvetch Root drying processing as claimed in claim 1, is characterized in that: described stepping is according to diameter 3cm, 2cm, 1cm and fall into three classes below; The drying condition of described microwave drying oven is 57 ~ 65W, dry 20 ~ 30 min; The drying condition of described infrared drying oven is 1000 ~ 3000 W, dry 15 ~ 20 min.
3. the method for Milkvetch Root drying processing as claimed in claim 1 or 2, it is characterized in that: the drying condition of described microwave drying oven is 61W, dry 20 min, the drying condition of described infrared drying oven is 1000W, dry 20min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510360652.0A CN104906176A (en) | 2015-06-26 | 2015-06-26 | Method for drying medicinal material radix astragali |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510360652.0A CN104906176A (en) | 2015-06-26 | 2015-06-26 | Method for drying medicinal material radix astragali |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104906176A true CN104906176A (en) | 2015-09-16 |
Family
ID=54075894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510360652.0A Pending CN104906176A (en) | 2015-06-26 | 2015-06-26 | Method for drying medicinal material radix astragali |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104906176A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105267606A (en) * | 2015-11-09 | 2016-01-27 | 天津大学 | Thunberg fritillary bulb processing method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101125126A (en) * | 2006-08-16 | 2008-02-20 | 丛繁滋 | Method for preparing medical freeze-dried powder (injection) preparation |
-
2015
- 2015-06-26 CN CN201510360652.0A patent/CN104906176A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101125126A (en) * | 2006-08-16 | 2008-02-20 | 丛繁滋 | Method for preparing medical freeze-dried powder (injection) preparation |
Non-Patent Citations (2)
| Title |
|---|
| 王俊等: "微波远红外联合干燥黄桃的试验研究", 《浙江农业学报》 * |
| 王孝涛等: "《中药采制与炮制技术》", 31 January 2000, 华夏出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105267606A (en) * | 2015-11-09 | 2016-01-27 | 天津大学 | Thunberg fritillary bulb processing method |
| CN105267606B (en) * | 2015-11-09 | 2019-04-16 | 天津大学 | A kind of processing method of fritillaria thunbergii |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104906175A (en) | Method for drying medicinal material radix astragali | |
| CN105641031A (en) | Compound pharmaceutical composition for strengthening body resistance and removing toxin, use and feed thereof | |
| CN110755542A (en) | Formula of uric acid-reducing gout-treating tea and preparation method thereof | |
| CN104906176A (en) | Method for drying medicinal material radix astragali | |
| CN105748398A (en) | Oral care solution and preparation method thereof | |
| CN106389562A (en) | Pudilan Xiaoyan granules, preparation method thereof, and product quality control method | |
| CN106177221A (en) | A kind of Chinese medicine composition for treating swine escherichia coli diarrhoea and preparation method thereof | |
| CN108653454A (en) | Purposes of the Chinese medicine composition of the black pearl containing thorn in treating porcine contagious pleuropneumonia | |
| CN105055485B (en) | Processing method of honeysuckle | |
| CN103877521A (en) | Pharmaceutical composition for preventing and treating chicken colibacillosis and preparation method of pharmaceutical composition | |
| CN107308248A (en) | A kind of Chinese herbal compound preparation beneficial to improving livestock and poultry production performance and preparation method thereof and application method | |
| CN107050100A (en) | Red stilbene Chinese medicine is taken after being infused in hot water or decoction medicine materical crude slice | |
| CN104857116B (en) | Application of the Chinese medicine garden burnet in preparing antifungal medicine synergist | |
| CN103919983A (en) | Antiviral and antibacterial mixture for preventing and treating upper respiratory tract infection of livestock and poultry | |
| CN104147121A (en) | Botanical aquatic product anesthesia/dormancy inducer and preparation method and application thereof | |
| CN105267279B (en) | The Chinese materia medica preparation and preparation method thereof for preventing and treating ascospherosis of bee | |
| CN1284553C (en) | Application of five-finger fig in preparing adjuvant for treating hepatitis and health foods | |
| CN102614349A (en) | Traditional Chinese medical composition for prevention and treatment of A type clostridiosis welchii, extract, preparation and preparation method and application | |
| CN108743693A (en) | A kind of piglet health care growth promotion Chinese medicine composition, preparation method and applications | |
| CN103451021A (en) | Method for CO2 supercritical extraction of volatile oil of hyssopus officinalis whole grass and use of volatile oil | |
| CN115006496B (en) | Chinese herbal medicine composition for treating pneumonia of foxes, raccoon dogs and martens | |
| CN107334878A (en) | It is a kind of to be used to treat Chinese medicine composition of piglet necrotic enteritis and preparation method thereof caused by C.perfringens | |
| CN107375458A (en) | Prevent and treat Chinese medicine composition, the preparation method and application of chicken colibacillosis | |
| CN107854526A (en) | A kind of Chinese medicine composition for treating chicken colibacillosis and its preparation method and application | |
| CN101249153A (en) | Chinese and western drugs for curing bird bacillus coli disease and method of preparing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150916 |
|
| RJ01 | Rejection of invention patent application after publication |