CN104849247B - 基于dna与重金属离子错配原理检测重金属离子的方法 - Google Patents
基于dna与重金属离子错配原理检测重金属离子的方法 Download PDFInfo
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- CN104849247B CN104849247B CN201510176917.1A CN201510176917A CN104849247B CN 104849247 B CN104849247 B CN 104849247B CN 201510176917 A CN201510176917 A CN 201510176917A CN 104849247 B CN104849247 B CN 104849247B
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- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 50
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- 150000002500 ions Chemical class 0.000 claims description 34
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 13
- 239000004202 carbamide Substances 0.000 claims description 7
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 6
- 239000013307 optical fiber Substances 0.000 claims description 4
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
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- 102000004190 Enzymes Human genes 0.000 abstract description 2
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- UDGUGZTYGWUUSG-UHFFFAOYSA-N 4-[4-[[2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]phenyl]diazenyl]-n-methylanilino]butanoic acid Chemical compound COC=1C=C(N=NC=2C=CC(=CC=2)N(C)CCCC(O)=O)C(OC)=CC=1N=NC1=CC=C([N+]([O-])=O)C=C1 UDGUGZTYGWUUSG-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
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- 238000002372 labelling Methods 0.000 description 2
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- 239000012086 standard solution Substances 0.000 description 2
- 244000174681 Michelia champaca Species 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003945 anionic surfactant Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
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- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN104849247B true CN104849247B (zh) | 2016-06-15 |
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Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
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CN2524241Y (zh) * | 2002-01-11 | 2002-12-04 | 谭玉山 | 应用相位跟踪法的光纤生物检测仪 |
CN1824797A (zh) * | 2005-12-28 | 2006-08-30 | 四川大学 | Dna荧光毛细生物传感器及其制备方法 |
US20090200486A1 (en) * | 2008-02-13 | 2009-08-13 | Nianqiang Wu | Quantum dot-DNA-metallic nanoparticle ensemble as fluorescent nanosensor system for multiplexed detection of heavy metals |
KR101185973B1 (ko) * | 2010-08-23 | 2012-09-25 | 한국과학기술원 | 비정상적인 핵산 중합 효소의 활성을 이용한 금속이온의 검출방법 및 이를 이용한 논리게이트 |
CN102200510A (zh) * | 2011-04-13 | 2011-09-28 | 上海出入境检验检疫局机电产品检测技术中心 | 利用t-t错配的dna探针荧光测定汞离子浓度的方法 |
CN102912011A (zh) * | 2012-08-24 | 2013-02-06 | 中国科学院上海微系统与信息技术研究所 | 基于寡核苷酸链的荧光增强型Hg2+检测芯片及方法 |
CN103160504A (zh) * | 2013-03-01 | 2013-06-19 | 上海交通大学 | 用于重金属铅的快速检测的核酸荧光探针及其检测方法 |
CN103389293B (zh) * | 2013-07-26 | 2015-11-04 | 中国人民大学 | 一种二价汞离子的检测方法 |
CN103667448A (zh) * | 2013-11-05 | 2014-03-26 | 中国科学院深圳先进技术研究院 | 双功能适配体检测试剂盒以及检测方法 |
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Effective date of registration: 20190307 Address after: 251100 Qilu high tech Development Zone, Qihe County, Dezhou, Shandong Patentee after: Shandong Bi Quan Environmental Engineering Technology Co., Ltd. Address before: Room 108, Training Base Office Building, Shandong Vocational College, 62 Jiefang Road, Jinan City, Shandong Province Patentee before: Liu Xiaoyong |
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Denomination of invention: Method for detecting heavy metal ions based on the mismatch principle of DNA and heavy metal ions Effective date of registration: 20211119 Granted publication date: 20160615 Pledgee: Shandong Qihe Rural Commercial Bank Co.,Ltd. Pledgor: SHANDONG BIQUAN ENVIRONMENT ENGINEERING TECHNOLOGY Co.,Ltd. Registration number: Y2021980012821 |
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