CN104837991A - 来自菜豆壳球孢菌的果胶降解酶及其用途 - Google Patents
来自菜豆壳球孢菌的果胶降解酶及其用途 Download PDFInfo
- Publication number
- CN104837991A CN104837991A CN201380054070.9A CN201380054070A CN104837991A CN 104837991 A CN104837991 A CN 104837991A CN 201380054070 A CN201380054070 A CN 201380054070A CN 104837991 A CN104837991 A CN 104837991A
- Authority
- CN
- China
- Prior art keywords
- sequence
- seq
- polypeptide
- enzyme
- mixing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01171—Rhamnogalacturonan hydrolase (3.2.1.171), i.e. rhamnogalacturonase
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01011—Pectinesterase (3.1.1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
- C12Y402/02002—Pectate lyase (4.2.2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
- C12Y402/02023—Rhamnogalacturonan endolyase (4.2.2.23)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Textile Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261683914P | 2012-08-16 | 2012-08-16 | |
| US61/683914 | 2012-08-16 | ||
| PCT/US2013/055198 WO2014028772A2 (en) | 2012-08-16 | 2013-08-15 | Pectin degrading enzymes from macrophomina phaseolina and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104837991A true CN104837991A (zh) | 2015-08-12 |
Family
ID=50101609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380054070.9A Pending CN104837991A (zh) | 2012-08-16 | 2013-08-15 | 来自菜豆壳球孢菌的果胶降解酶及其用途 |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US9765322B2 (https=) |
| EP (1) | EP2885407B1 (https=) |
| JP (1) | JP6644547B2 (https=) |
| KR (1) | KR102043363B1 (https=) |
| CN (1) | CN104837991A (https=) |
| AU (1) | AU2013302535B2 (https=) |
| DK (1) | DK2885407T3 (https=) |
| ES (1) | ES2674922T3 (https=) |
| HK (1) | HK1213597A1 (https=) |
| IN (1) | IN2015DN02049A (https=) |
| MY (1) | MY169809A (https=) |
| PL (1) | PL2885407T3 (https=) |
| PT (1) | PT2885407T (https=) |
| TR (1) | TR201809053T4 (https=) |
| WO (1) | WO2014028772A2 (https=) |
| ZA (1) | ZA201501635B (https=) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004074468A1 (en) * | 2002-05-30 | 2004-09-02 | Dsm Ip Assets B.V. | Novel pectinases and uses thereof |
| CN1662650A (zh) * | 2002-05-14 | 2005-08-31 | 诺和酶股份有限公司 | 果胶酸裂解酶变体 |
| WO2010070146A1 (en) * | 2008-12-19 | 2010-06-24 | Danisco A/S | Process for production of an enzyme product |
| CN101955921A (zh) * | 1995-03-17 | 2011-01-26 | 诺沃奇梅兹有限公司 | 新的内切葡聚糖酶 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| GB8702475D0 (en) | 1987-02-04 | 1987-03-11 | Ciba Geigy Ag | Expression system |
| ES2063162T5 (es) | 1988-07-28 | 2002-11-16 | Novartis Ag | Nuevo sistema de expresion. |
| DE3908813A1 (de) | 1989-03-17 | 1990-09-20 | Roehm Gmbh | Verfahren zur expression eines aus aspergillus niger stammenden gens in einem aspergillus |
| IL108156A0 (en) | 1992-12-23 | 1994-04-12 | Novo Nordisk As | An enzyme with polygalacturonase activity |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| CN101659926A (zh) | 1994-06-30 | 2010-03-03 | 诺沃奇梅兹有限公司 | 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子 |
| US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
| US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| JP3534607B2 (ja) | 1997-04-09 | 2004-06-07 | 花王株式会社 | 洗浄剤組成物 |
| US7214786B2 (en) * | 2000-12-14 | 2007-05-08 | Kovalic David K | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
| US10676751B2 (en) * | 2008-02-29 | 2020-06-09 | The Trustees Of The University Of Pennsylvania | Production and use of plant degrading materials |
| WO2010101158A1 (ja) | 2009-03-02 | 2010-09-10 | 住友商事株式会社 | クロストリジウム セルロボランス由来新規遺伝子及びその利用 |
-
2013
- 2013-08-15 IN IN2049DEN2015 patent/IN2015DN02049A/en unknown
- 2013-08-15 MY MYPI2015000387A patent/MY169809A/en unknown
- 2013-08-15 EP EP13829902.9A patent/EP2885407B1/en active Active
- 2013-08-15 WO PCT/US2013/055198 patent/WO2014028772A2/en not_active Ceased
- 2013-08-15 KR KR1020157006677A patent/KR102043363B1/ko not_active Expired - Fee Related
- 2013-08-15 TR TR2018/09053T patent/TR201809053T4/tr unknown
- 2013-08-15 DK DK13829902.9T patent/DK2885407T3/en active
- 2013-08-15 PT PT138299029T patent/PT2885407T/pt unknown
- 2013-08-15 HK HK16101535.2A patent/HK1213597A1/zh unknown
- 2013-08-15 AU AU2013302535A patent/AU2013302535B2/en not_active Ceased
- 2013-08-15 PL PL13829902T patent/PL2885407T3/pl unknown
- 2013-08-15 US US14/421,729 patent/US9765322B2/en not_active Expired - Fee Related
- 2013-08-15 ES ES13829902.9T patent/ES2674922T3/es active Active
- 2013-08-15 CN CN201380054070.9A patent/CN104837991A/zh active Pending
- 2013-08-15 JP JP2015527642A patent/JP6644547B2/ja not_active Expired - Fee Related
-
2015
- 2015-03-10 ZA ZA2015/01635A patent/ZA201501635B/en unknown
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955921A (zh) * | 1995-03-17 | 2011-01-26 | 诺沃奇梅兹有限公司 | 新的内切葡聚糖酶 |
| CN1662650A (zh) * | 2002-05-14 | 2005-08-31 | 诺和酶股份有限公司 | 果胶酸裂解酶变体 |
| WO2004074468A1 (en) * | 2002-05-30 | 2004-09-02 | Dsm Ip Assets B.V. | Novel pectinases and uses thereof |
| WO2010070146A1 (en) * | 2008-12-19 | 2010-06-24 | Danisco A/S | Process for production of an enzyme product |
Non-Patent Citations (4)
| Title |
|---|
| Extra-cellular pectic enzymes of macrophomina phaseolina-the incitant of root-rot of sesamum indicum;H.C.Dube,H.N.Gour;《Proceedings of the Indian National Science Academy,B》;19751231;第41卷(第6期);第576-579页 * |
| H.C.DUBE,H.N.GOUR: "Extra-cellular pectic enzymes of macrophomina phaseolina-the incitant of root-rot of sesamum indicum", 《PROCEEDINGS OF THE INDIAN NATIONAL SCIENCE ACADEMY,B》 * |
| MA L.-J.J. ET AL.: "Accession NO. xp_003000083,"pectate lyase [Verticillium alfalfae VaMs.102]"", 《GENBANK》 * |
| MA,L.-J.J.. ET AL.: "Accession NO. XM_003000037,"Verticillium albo-atrum VaMs.102 pectate lyase,mRNA"", 《GENBANK》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014028772A2 (en) | 2014-02-20 |
| EP2885407B1 (en) | 2018-06-13 |
| BR112015003086A2 (pt) | 2018-06-26 |
| WO2014028772A3 (en) | 2014-05-08 |
| DK2885407T3 (en) | 2018-07-16 |
| US20150259667A1 (en) | 2015-09-17 |
| JP6644547B2 (ja) | 2020-02-12 |
| KR20150042849A (ko) | 2015-04-21 |
| AU2013302535B2 (en) | 2018-12-06 |
| JP2015529071A (ja) | 2015-10-05 |
| MY169809A (en) | 2019-05-16 |
| AU2013302535A1 (en) | 2015-03-26 |
| TR201809053T4 (tr) | 2018-07-23 |
| EP2885407A4 (en) | 2016-05-11 |
| PT2885407T (pt) | 2018-07-02 |
| KR102043363B1 (ko) | 2019-11-11 |
| EP2885407A2 (en) | 2015-06-24 |
| IN2015DN02049A (https=) | 2015-08-14 |
| ZA201501635B (en) | 2016-01-27 |
| ES2674922T3 (es) | 2018-07-05 |
| HK1213597A1 (zh) | 2016-07-08 |
| PL2885407T3 (pl) | 2018-09-28 |
| US9765322B2 (en) | 2017-09-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9902983B2 (en) | Agarooligosaccharide hydrolase and method for producing 3,6-anhydro-L-galactose and galactose from agarose by using same | |
| CN111748535B (zh) | 一种丙氨酸脱氢酶突变体及其在发酵生产l-丙氨酸中的应用 | |
| CN111836900A (zh) | 从海藻生产葡萄糖及昆布多糖寡糖的新型β-葡萄糖苷酶 | |
| CN104531637B (zh) | 一种β-葡萄糖苷酶和β-葡萄糖苷酶突变体及应用 | |
| CN106133144A (zh) | 使用酶的4‑氨基肉桂酸的制备方法 | |
| CN102559638A (zh) | 一种碱性果胶酶pla及其基因和应用 | |
| CN102719417A (zh) | 一种耐高温阿拉伯呋喃糖苷酶Abf51B8及其基因和应用 | |
| AU2013302536B2 (en) | Lignin degrading enzymes from Macrophomina phaseolina and uses thereof | |
| Tang et al. | Cloning and expression of cellulase gene EG1 from Rhizopus stolonifer var. reflexus TP-02 in Escherichia coli | |
| CN104560917B (zh) | 一种β-葡萄糖苷酶和β-葡萄糖苷酶突变体及应用 | |
| JP6616687B2 (ja) | Macrophominaphaseolina由来のセルロースおよび/またはヘミセルロース分解酵素およびその使用 | |
| CN107779443B (zh) | 纤维二糖水解酶突变体及其应用 | |
| CN104837991A (zh) | 来自菜豆壳球孢菌的果胶降解酶及其用途 | |
| CN104789546A (zh) | 一种脱乙酰基酶突变体及其应用 | |
| BR112015003086B1 (pt) | Sequência de polinucleotídeo recombinante, vetor de expressão recombinante e célula hospedeira procariótica geneticamente engenheirada | |
| CN110982831B (zh) | 基因AlgL23具有冷适应性的用途 | |
| JP4670048B2 (ja) | 生分解性プラスチック分解酵素をコードする遺伝子の取得方法、それにより得られる新規遺伝子および酵素 | |
| TWI609961B (zh) | 用於生產重組酵素之核酸建構物、重組型表現載體及其方法 | |
| BR112015003085B1 (pt) | Molécula de ácido nucleico recombinante, constructo recombinante de expressão e célula hospedeira procariótica engenheirada geneticamente |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1213597 Country of ref document: HK |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150812 |
|
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: WD Ref document number: 1213597 Country of ref document: HK |