CN104830663A - Double room hypha test apparatus and double room hypha test method - Google Patents
Double room hypha test apparatus and double room hypha test method Download PDFInfo
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- CN104830663A CN104830663A CN201510173021.8A CN201510173021A CN104830663A CN 104830663 A CN104830663 A CN 104830663A CN 201510173021 A CN201510173021 A CN 201510173021A CN 104830663 A CN104830663 A CN 104830663A
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- room
- mycelia
- hypha
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- air buffer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention relates to the field of arbuscular mycorrhiza fungi test apparatus, particularly to a double room hypha test apparatus and a double room hypha test method. The double room hypha test apparatus comprises a plant room, a filtration screen, an air separation layer and a hypha room from top to bottom, wherein the upper portion of the plant room is provided with an opening, the edge side of the plant room is sealed, the filtration screen and the air separation layer are arranged on the bottom portion of the plant room, the upper portion of the hypha room is provided with an opening, the edge side of the hypha room is provided with a ventilation gas permeation hole, the bottom portion of the hypha room is the completely-closed structure, and the plant room and the hypha room are connected through a bolt. According to the present invention, with the air separation layer method, the root system and the hypha can be well separated; and the upper compartment and the lower compartment are adopted, such that the gravity effect can be well utilized, the hypha growth is easily achieved, the nutrient diffusion influence between the two rooms due to the mass flow can be reduced, and the good test effect is achieved.
Description
Technical field
The present invention relates to bush mycorrhizal fungi testing apparatus field, be specifically related to a kind of two Room mycelia testing apparatus and methods.
Background technology
Arbuscular mycorrhiza (Arbuscular mycorrhiza, AM) fungi is the mycorrhizal type the most widely that distributes, and the angiosperm on land 80% ~ 90% can form arbuscular mycorrhiza.The scope that plant absorbs nutrient from soil is limited, and after AM fungal infection plant, the outer mycelia of root extensively can stretch in soil through rhizosphere nutrient owes lacks district, forms Hyphae links, expands the nutrition absorption scope of root system.
AM fungi and Root Symbiont communication with one another under field conditions (factors), the impact that very difficult differentiation root system or mycelia absorb soil nutrient.Li Xiaolin (1990) adopts the testing apparatus of three Room, utilizes the nylon wire of 30 μm root and mycelia to be separated, establishes Hyphae links.The method is that research mycelia and the physiological acoustic signals of Hyphae links thereof provide a useful approach.But it still can not get rid of the impact that the measure such as external microbe and fertigation causes, in order to study the physiological acoustic signals of mycelia further, a kind of Hyphae links environment without miscellaneous bacteria must be set up, the root formed under in vitro dual culture condition in symbiote and hypha separation are come, mycelia is made to enter mycelia room, and root is stoped in bacterium plant room, do not allow the two mix, for the physio-biochemical characteristics of further investigation mycorhiza mycelia provide new techniques and methods.The testing apparatus of above-mentioned three Room utilizes the nylon wire of 30 μm root and mycelia to be separated, and forms mycelia room and establishes Hyphae links.But also there is shortcoming: still can not get rid of the matter stream of element between mycelia room and plant room that in soil, movability is strong and spread the impact caused.
A traditional point chamber device is left-right situs, and like this need plant to form root pad in side, then mycelia arrives mycelia room from the side, effectively can not control the impact of matter stream diffusion.
Summary of the invention
One of main purpose of the present invention is to solve the problem, and then provides a kind of two Room mycelia testing apparatus and methods, and root system and mycelia can either separate by this device, can also reduce the impact of matter stream diffusion simultaneously.
The adopted technical scheme that solves the problem is:
A kind of two Room mycelia testing apparatuss, described device from top to bottom comprises: plant room, filtering net, air buffer and mycelia room; Described plant room upper opening, avris is closed, and described filtering net and described air buffer are arranged on bottom described plant room; Described mycelia room upper opening, avris is provided with ventilation holes, and bottom is full-closed structure; Described plant room and described mycelia room are bolted.
Further, described air buffer is the hard plastic board of band circular hole, and described air buffer thickness is 1.5 ~ 2.5mm, and the diameter of described circular hole is 1.5 ~ 2.5mm, and pitch-row is 1.5 ~ 2.5mm.
Further, described air buffer thickness is 2mm, and the diameter of described circular hole is 2mm, and pitch-row is 2mm.
Further, described hard plastic board is any one in PVC board, polycarbonate plate or poly (methyl methacrylate) plate.
Further, described filtering net is on described air buffer.
Further, described plant room and mycelia room can be cylindrical, rectangular parallelepiped or cube structure.
A kind of two Room mycelia test methods, step is: step one: by plant growing in plant room; Step 2: cultivation soil and stalk are placed in mycelia room, and described stalk is placed on described cultivation soil, puts into soil organisms as required in mycelia room; Step 3: described plant room is positioned on described mycelia room, with bolt, whole device is fixed; Step 4: carry out experimental observation.
Further, the soil organisms described in described step 3 comprises: microfauna fauna, individual lengths below 0.2 millimeter, mainly protozoon; Mesofauna, individual lengths, between 0.2 ~ 10 millimeter, mainly contains nematode, acarina (Acarina) and Collembola (Collemb-ola), light up subclass (Pauropoda), comprehensive subclass (Symphyla), Thysanura (Thysanura), Protura (Protura) etc.; Macrofauna, individual lengths is more than 10 millimeters, mainly contain earthworm, enchytrae (Enchytraeus), centipede (Scolopendra), ant, termite, Diptera (Diptera) larva and the various insects based on beetle, and minority Crustachia (Crustacea) and Gastropoda (Gastropoda) animal.
Beneficial effect of the present invention is:
1, the advantage of combined: plant room (Root Compartment) planting plants above, Arbuscular Mycorrhizal Fungi fungi can treat mycelia grow after (within general two weeks, just can infect) splice with mycelia room below again.
2, the advantage of air buffer: hole above hard plastic board bottom plant room, form air buffer, mycelia can arrive mycelia room through gas cloud, will avoid the direct contact between upper and lower two Room like this, root system and mycelia can either be separated, the impact of matter stream diffusion can also be reduced simultaneously.
3, the advantage of upper lower compartment: a traditional point chamber device is left-right situs, like this need plant to form root pad in side, then mycelia arrives mycelia room from the side, after making lower compartment into, due to action of gravity, mycelia can grow downwards by nature, more easily arrives mycelia room; In addition, due to action of gravity, the nutrient ion in mycelia room below can not flow to by matter the plant room reached above, thus avoids the impact of matter stream diffusion to greatest extent.
In sum, a kind of two Room mycelia testing apparatuss and method can the Hyphae links effects of comparatively accurate study plant, eliminate the impact of non-test factor to greatest extent.Meanwhile, can add the soil animals such as earthworm in mycelia room, the effect of research Hyphae links soil animal, makes research range greatly widen.Upper lower compartment can add the material wanting to study more easily, such as isotropic substance under the prerequisite as far as possible reducing testing error in lower floor mycelia room, or other trace element etc., play good isolation action.
Accompanying drawing explanation
Figure 1 shows that apparatus of the present invention connect rear structural representation.
Figure 2 shows that the structural representation of plant room of the present invention.
Figure 3 shows that the structural representation of mycelia room of the present invention.
Figure 4 shows that the vertical view of plant room of the present invention.
Figure 5 shows that the upward view of plant room of the present invention.
Figure 6 shows that the bottom A-A sectional view of plant room of the present invention.
Label shown in figure is: 1 for be the first edge, 12 be in plant room, 11 first circular hole, 13 be filtering net, 14 be air buffer, 2 be mycelia room, 21 second edges, 22 the 3rd edges, 23 be the 3rd circular hole, 24 be the second circular hole, 25 are ventilation holes.
Embodiment
Hereafter will describe embodiments of the invention in detail by reference to the accompanying drawings.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they can mutually be combined thus be reached better technique effect.
Embodiment 1
As Fig. 1, Fig. 4 and Fig. 5 are known: a kind of two Room mycelia testing apparatuss, from top to bottom comprise: plant room 1, filtering net 13, air buffer 14 and mycelia room 2; Described plant room 1 is cylindrical and upper opening, and avris is closed, and bottom is provided with filtering net 13 and air buffer 14; Described mycelia room 2 is the lower unit of described testing apparatus, and described mycelia room 2 is cylindrical structural and upper opening, and avris is provided with ventilation holes 25, and bottom is full-closed structure; Described plant room 1 is bolted fixing with described mycelia room 2, detachably combines.
As shown in Figure 6, filtering net 13 is on described air buffer, and sizing grid is homogeneous, and grid is evenly arranged; Described air buffer 14 is the hard plastic board of band circular hole; Further, hard plastic board is any one in PVC board, polycarbonate plate or poly (methyl methacrylate) plate; Preferred hard plastic board is PVC board; Described air buffer 14 thickness is 1.5 ~ 2.5mm, and circular hole comprises the first circular hole 12, second circular hole 24 the 3rd circular hole 23, and the diameter of described circular hole is 1.5 ~ 2.5mm, and pitch-row is 1.5 ~ 2.5mm; Preferred air buffer thickness is 2mm, and the diameter of described circular hole is 2mm, and pitch-row is 2mm.
As Fig. 2 and Fig. 3 is known, described circular hole is corresponding to be respectively arranged on the edge on each limit, first edge 11 is provided with the first circular hole 12, second edge 21 is provided with the second circular hole 24,3rd edge 22 is provided with the 3rd circular hole 23, wherein the quantity of the first circular hole 12 and the second circular hole 24 and position corresponding, the first circular hole 12 and the second circular hole 24 are connected and fixed by bolt, the 3rd circular hole 23 is fixed by the position of bolt by whole device.
Wherein plant room 1 and mycelia room 2 can also be rectangular parallelepiped or cube structure.
Embodiment 2
A kind of two Room mycelia test methods are: step one: by plant growing in plant room 1; Step 2: be placed among mycelia room 2 by cultivation soil and stalk, described stalk is placed on described cultivation soil, puts into earthworm or other soil animals as required in mycelia room 2; Step 3: described plant room 1 is positioned on described mycelia room 2, with bolt, whole device is fixed; Step 4: carry out experimental observation.
Carry out cultivating and studying in plant device after mounting, plant roots and stems major part is in plant room 1, wherein mycelia enters mycelia room 2 through the space of filtering net 13 and air buffer 14, cultivation soil, stalk and fertilizer etc. are covered with at mycelia room 2 middle berth, and in mycelia room 2, add research with soil animal, the influence and effect of various soil animal to mycelia can be studied by putting into different soil animals.
A kind of two Room mycelia testing apparatuss of the present invention and method can the Hyphae links effects of comparatively accurate study plant, get rid of the impact of non-empirical factor to greatest extent.Meanwhile, can add earthworm or other soil organismss such as nematode, springtail in mycelia room, the effect of research Hyphae links soil animal, makes research range greatly widen.Upper lower compartment can be convenient and with errors do not add the material wanting to study in lower floor mycelia room, plays isolation action very also.Such as isotropic substance, or other trace element etc.
Although give some embodiments of the present invention, it will be understood by those of skill in the art that without departing from the spirit of the invention herein, can change embodiment herein.Above-described embodiment is exemplary, should using embodiment herein as the restriction of interest field of the present invention.
Claims (8)
1. two Room mycelia testing apparatuss, it is characterized in that, described device from top to bottom comprises: plant room, filtering net, air buffer and mycelia room; Described plant room upper opening, avris is closed, and described filtering net and described air buffer are arranged on bottom described plant room; Described mycelia room upper opening, avris is provided with ventilation holes, and bottom is full-closed structure; Described plant room and described mycelia room are bolted.
2. a kind of two Room mycelia testing apparatuss as claimed in claim 1, is characterized in that, described air buffer is the hard plastic board of band circular hole, and described air buffer thickness is 1.5 ~ 2.5mm, and the diameter of described circular hole is 1.5 ~ 2.5mm, and pitch-row is 1.5 ~ 2.5mm.
3. a kind of two Room mycelia testing apparatuss as claimed in claim 2, it is characterized in that, described air buffer thickness is 2mm, and the diameter of described circular hole is 2mm, and pitch-row is 2mm.
4. a kind of two Room mycelia testing apparatuss as claimed in claim 2, it is characterized in that, described hard plastic board is any one in PVC board, polycarbonate plate or poly (methyl methacrylate) plate.
5. a kind of two Room mycelia testing apparatuss as claimed in claim 1, it is characterized in that, described filtering net is on described air buffer.
6. a kind of two Room mycelia testing apparatuss as claimed in claim 1, is characterized in that, described plant room and mycelia room can be cylindrical, rectangular parallelepiped or cube structure.
7. two Room mycelia test methods, it is characterized in that, step is:
Step one: by plant growing in plant room;
Step 2: cultivation soil and stalk are placed in mycelia room, and described stalk is placed on described cultivation soil, puts into soil organisms as required in mycelia room;
Step 3: described plant room is positioned on described mycelia room, with bolt, whole device is fixed;
Step 4: carry out experimental observation.
8. a kind of two Room mycelia test methods as claimed in claim 8, it is characterized in that, the soil organisms described in described step 3 is any one or several combinations in earthworm, nematode, springtail, acarina, Collembola, candle subclass, comprehensive subclass, Thysanura, Protura, enchytrae, centipede, ant, termite, Diptera larvae or beetle.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510173021.8A CN104830663A (en) | 2015-04-13 | 2015-04-13 | Double room hypha test apparatus and double room hypha test method |
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| CN201510173021.8A CN104830663A (en) | 2015-04-13 | 2015-04-13 | Double room hypha test apparatus and double room hypha test method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105349410A (en) * | 2015-11-30 | 2016-02-24 | 苏州钧隆塑胶有限公司 | Bidirectional bacterial culture bottle |
| CN105875202A (en) * | 2016-06-13 | 2016-08-24 | 蔡志闯 | Inoculation mechanism, strain containers and method for culturing edible mushrooms |
| CN106034801A (en) * | 2016-06-29 | 2016-10-26 | 西安科技大学 | Root system damage simulation device |
| CN107041235A (en) * | 2017-03-27 | 2017-08-15 | 贵州大学 | Utilize nutrient device between atmospheric carbon dioxide regulation and control mycorrhizal fungi transfering plant |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105349410A (en) * | 2015-11-30 | 2016-02-24 | 苏州钧隆塑胶有限公司 | Bidirectional bacterial culture bottle |
| CN105875202A (en) * | 2016-06-13 | 2016-08-24 | 蔡志闯 | Inoculation mechanism, strain containers and method for culturing edible mushrooms |
| CN106034801A (en) * | 2016-06-29 | 2016-10-26 | 西安科技大学 | Root system damage simulation device |
| CN107041235A (en) * | 2017-03-27 | 2017-08-15 | 贵州大学 | Utilize nutrient device between atmospheric carbon dioxide regulation and control mycorrhizal fungi transfering plant |
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Application publication date: 20150812 |