CN203289960U - Device for in-situ study of forest tree rhizosphere effect - Google Patents

Device for in-situ study of forest tree rhizosphere effect Download PDF

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Publication number
CN203289960U
CN203289960U CN2013203426416U CN201320342641U CN203289960U CN 203289960 U CN203289960 U CN 203289960U CN 2013203426416 U CN2013203426416 U CN 2013203426416U CN 201320342641 U CN201320342641 U CN 201320342641U CN 203289960 U CN203289960 U CN 203289960U
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China
Prior art keywords
rhizosphere
soil
root
root growth
organic glass
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Expired - Fee Related
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CN2013203426416U
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Chinese (zh)
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郭海超
吴小平
王文斌
罗雪华
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Rubber Research Institute Chinese Academy Tropical Agricultural Sciences
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Rubber Research Institute Chinese Academy Tropical Agricultural Sciences
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Abstract

The utility model provides a device for in-situ study of a forest tree rhizosphere effect. The device is composed of a root growth room and a rhizosphere soil room, and 2cm of one end of the rhizosphere soil room is inserted into the root growth room. The root growth room comprises a transparent organic glass tube and an isolation screen. The rhizosphere soil room comprises a transparent organic glass tube and an isolation screen. The device is suitable for the in-situ study of the perennial forest tree rhizosphere effect, and can distinguish and collect soil samples with different distances from a root conveniently, effectively and accurately.

Description

A kind of device of original position research forest rhizosphere effect
Technical field
The utility model relates to a kind of device of original position research forest rhizosphere effect, is specifically related to a kind of be used to carrying out the device of the forest rhizosphere micro-region effects such as original position research bamboo grows.
Background technology
Rhizosphere generally refers to the microbending soil of the not enough 1mm of root system and Soil Interface to several millimeters scopes, factor relevant (Liu Zhiyu etc., 1997 such as the specific distribution of its scope and floristics, soil types, soil moisture content, root system and root exudates; Hinsinger, 2001).Plant is not only supplied with it by root system from absorption nutrient and moisture soil and grows, and constantly by root system, in soil, secrete various chemical substances again in its process of growth, and between soil, constantly carrying out material recycle and energy flow (Hinsinger etc., 2009).Due to stronger microbial environment in the effect of Gen Mao and root secretion and rhizosphere scope, make the characteristics such as physics, chemistry and biology of soil in rhizosphere micro-region obviously be different from non-rhizosphere soil.At first moisture in soil and nutrient must could arrive the root table through this micro-territory, and then are Root Absorption utilization (Feng Zhimin etc., 2008).Therefore, the environmental aspect in this micro-territory, directly affect moisture, nutrient availability and availability.Since 1904 " rhizosphere " word proposes, rhizosphere to plant nutrition, grow and the effect research such as pathology more and more is subject to global extensive attention (Hinsinger etc., 2009; Neumann etc., 2009; Zhang etc., 2010).Therefore, be necessary to strengthen the rhizosphere effect researchs such as nutrient, moisture and edaphon.
Rhizosphere micro-region research at present focuses mostly on the annual crop of the shallow roots such as wheat, barley, oat, corn, rape, perennial ryegrass, adopts root case or root bag to carry out (Zhao Bing etc., 1999) more.And the perennial plants such as forest belong to the system of taproot due to root system, and Root Distribution is wide and the degree of depth is larger; Main root is grown vertically downward, general dark bundle to 150cm place, earth's surface or below, lateral root level or extending obliquely.Adopt root case method to realize that by seedling cultivation the rhizosphere micro-region differentiation often has limitation, can't truly reflect the rhizosphere effect of perennial forest under actual conditions.Present perennial forest rhizosphere soil sampling adopts method to distinguish (Liu Zhiyu etc. to root system rhizosphere and non-rhizosphere, 1997), but the kind method often is subjected to the impact of the factors such as soil moisture content, the soil texture, and this method can't realize differentiation and the collection of root table different distance soil specimen.Therefore, lack the limiting factor that suitable research method has become the chemical behavior researchs such as restriction perennial forest rhizosphere nutrient, moisture and microorganism.
Summary of the invention
The purpose of this utility model is to provide a kind of device that is applicable to perennial forest root table different distance micro-region soil sampling.
In order to realize the purpose of this utility model, the device of a kind of original position research of the utility model forest rhizosphere effect is comprised of root growth chamber and rhizosphere soil chamber, and rhizosphere soil chamber one end is inserted into the indoor 2cm of root growth.
Described root growth chamber comprises transparent organic glass pipe and separation net, and transparent organic glass Guan Yiduan covers and covers separation net, and separation net adopts nylon wire, aperture 2mm; Transparent organic glass bore 15.7~15.9cm, the external diameter 16.3~16.5cm of root growth chamber, high 6.0cm.
Described rhizosphere soil chamber comprises transparent organic glass pipe and separation net, and transparent organic glass pipe two ends are covered and covered separation net, and separation net all adopts nylon wire, and aperture is 48 μ m; Transparent organic glass bore 14.9~15.1cm, external diameter 15.5~15.7cm, high 5.0cm.
The device of a kind of original position research of the utility model forest rhizosphere effect, be applicable to the research of perennial forest rhizosphere effect, during actual the use, in root growth chamber and the indoor corresponding soils of all inserting of rhizosphere soil, the processing such as soil can apply fertilizer in advance accordingly, bacterial classification inoculation, rhizosphere soil chamber one end is inserted in the root growth chamber, can realize the circulation of the indoor nutrient of root growth chamber and rhizosphere soil and moisture.This device can be imbedded the soil layer of appropriate depth according to actual needs, and gathers this device according to experimental establishment in good time.The nylon wire of this device root growth chamber employing aperture 2mm covers and covers, and can effectively stop penetrating of the thick root of forest (diameter > 2mm), and (feeding root such as diameter≤2mm) passes nylon wire, can realize the enrichment of feeding root to make radicula.Two ends, rhizosphere soil chamber all adopt the nylon wire illiteracy of aperture 48 μ m to cover, and can effectively stop penetrating of root system and Gen Mao, but can not affect the migration of nutrient, moisture, root exudates and microorganism.When sampling, the rhizosphere soil chamber is taken off, freezing cutting or In situcut all can obtain easily apart from differentiation and the collection of root different distance soil specimen, increased the accuracy of sampling and the integrality of soil layer, and sampling are convenient rapidly.
The accompanying drawing explanation
Fig. 1: the device of original position research forest rhizosphere effect.
Fig. 2: root growth chamber.
Fig. 3: rhizosphere soil chamber.
In figure: 1. root growth chamber separation net; 2. root growth chamber; 3. rhizosphere soil chamber separation net; 4. rhizosphere soil chamber.
Embodiment
Following examples are used for the utility model is described, but are not used for limiting scope of the present utility model.
When the device of a kind of original position research of the utility model forest rhizosphere effect is implemented, as shown in Figure 1-Figure 3, comprise root growth chamber separation net (1), root growth chamber (2), rhizosphere soil chamber separation net (3), rhizosphere soil chamber (4).Transparent organic glass pipe, internal diameter 15.8cm, external diameter 16.4cm, high 6.0cm are adopted in the root growth chamber; The transparent organic glass Guan Yiduan of root growth chamber covers and covers separation net, and separation net adopts nylon wire, aperture 2mm.Two ends, rhizosphere soil chamber are covered and are covered separation net, and separation net all adopts nylon wire, and aperture is 48 μ m; Transparent organic glass pipe, internal diameter 15.0cm, external diameter 15.6cm, high 5.0cm are adopted in the rhizosphere soil chamber.
During application, requirement according to the forest experimental study, in the place of preparing to imbed forest rhizosphere device, the in-situ acquisition soil specimen, take back laboratory, measure the bulk density of soil and soil moisture content, air-dry ground 4mm sieve after, after apply fertilizer accordingly or microbial inoculant etc. processed, according to the actual bulk density of soil, respectively appropriate soil is filled in root growth chamber and rhizosphere soil chamber, and supplementary soil moisture to field actual water content.After soil was filled in the root growth chamber, an end illiteracy was covered separation net, and after soil was filled in the rhizosphere soil chamber, the two ends illiteracy was covered separation net, and rhizosphere soil chamber one end is inserted to the indoor 2cm of root growth.
In the place of preparing to imbed forest rhizosphere device, the soil layer of selecting root system to concentrate, the angle that original position is studied to the device of forest rhizosphere effect and ground is 45 ° to be laid, and makes the root growth chamber up, then earthing.During to off-test, excavate table soil, cut off top, root growth chamber illiteracy and cover the root system that adheres on separation net, withdrawing device, remove root growth chamber, top transparent organic glass pipe, rhizosphere soil chamber, bottom transparent organic glass pipe is whole freezing, pour out after soil and adopt slicing treatment can realize differentiation and the collection of root table different distance rhizosphere soil sample.
Although, above with a general description of the specific embodiments the invention having been done to detailed description, on the basis of the invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements on the basis of not departing from the invention spirit, all belong to the claimed scope of the invention.

Claims (5)

1. the device of an original position research forest rhizosphere effect, is characterized in that being comprised of root growth chamber and rhizosphere soil chamber, and rhizosphere soil chamber one end is inserted in the root growth chamber; Described root growth chamber comprises transparent organic glass pipe and separation net, and the rhizosphere soil chamber comprises transparent organic glass pipe and separation net.
2. the device of a kind of original position research forest rhizosphere effect according to claim 1, is characterized in that transparent organic glass bore 15.7~15.9cm, the external diameter 16.3~16.5cm of root growth chamber, high 6.0cm.
3. the device of a kind of original position research forest rhizosphere effect according to claim 1, is characterized in that the transparent organic glass Guan Yiduan illiteracy of root growth chamber is covered separation net, and separation net adopts nylon wire, aperture 2mm.
4. the device of a kind of original position research forest rhizosphere effect according to claim 1, is characterized in that transparent organic glass bore 14.9~15.1cm, the external diameter 15.5~15.7cm of rhizosphere soil chamber, high 5.0cm.
5. the device of a kind of original position research forest rhizosphere effect according to claim 1, is characterized in that the transparent organic glass pipe two ends illiteracy of rhizosphere soil chamber is covered separation net, and separation net all adopts nylon wire, and aperture is 48 μ m.
CN2013203426416U 2013-06-06 2013-06-06 Device for in-situ study of forest tree rhizosphere effect Expired - Fee Related CN203289960U (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104830663A (en) * 2015-04-13 2015-08-12 青岛农业大学 Double room hypha test apparatus and double room hypha test method
CN105393825A (en) * 2015-12-11 2016-03-16 浙江大学 Soil instant rhizosphere simulation culturing apparatus and application of same
CN106226136A (en) * 2016-08-09 2016-12-14 中国科学院东北地理与农业生态研究所 The device of a kind of herborization rhizosphere soil and utilize its method gathering rhizosphere soil
CN107941990A (en) * 2017-10-23 2018-04-20 中国环境科学研究院 Water plant root phosphorus uptake test device and method based on DGT technologies

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104830663A (en) * 2015-04-13 2015-08-12 青岛农业大学 Double room hypha test apparatus and double room hypha test method
CN105393825A (en) * 2015-12-11 2016-03-16 浙江大学 Soil instant rhizosphere simulation culturing apparatus and application of same
CN105393825B (en) * 2015-12-11 2018-12-28 浙江大学 A kind of instant rhizosphere simulation culture apparatus of soil and its application
CN106226136A (en) * 2016-08-09 2016-12-14 中国科学院东北地理与农业生态研究所 The device of a kind of herborization rhizosphere soil and utilize its method gathering rhizosphere soil
CN107941990A (en) * 2017-10-23 2018-04-20 中国环境科学研究院 Water plant root phosphorus uptake test device and method based on DGT technologies

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Granted publication date: 20131120

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