CN213875025U

CN213875025U - Culture apparatus suitable for perennial forest normal position rhizosphere soil sampling

Info

Publication number: CN213875025U
Application number: CN202022746239.7U
Authority: CN
Inventors: 薛欣欣; 王文斌; 张永发; 罗雪华; 赵春梅
Original assignee: Rubber Research Institute Chinese Academy Tropical Agricultural Sciences
Current assignee: Rubber Research Institute Chinese Academy Tropical Agricultural Sciences
Priority date: 2020-11-24
Filing date: 2020-11-24
Publication date: 2021-08-03
Anticipated expiration: 2030-11-24

Abstract

The utility model relates to a culture apparatus suitable for perennial forest normal position rhizosphere soil sample, including casing, first net piece and two at least second net pieces, be provided with in the casing and gather the chamber, it has the opening to gather the chamber, first net piece set up in casing and seal the opening, each second net piece interval set up in the casing, the aperture of first net piece is greater than the aperture of second net piece, first net piece and neighbouring second net piece interval sets up, gather the bottom in chamber and neighbouring second net piece interval sets up. The culture device suitable for perennial forest in-situ rhizosphere soil sampling can quickly and accurately collect rhizosphere soil and non-rhizosphere soil, cannot damage the growth of root systems in a large area, has the characteristics of simple structure and convenient operation, and is particularly suitable for actual collection work in field fields.

Description

Culture apparatus suitable for perennial forest normal position rhizosphere soil sampling

Technical Field

The utility model relates to a rhizosphere soil sampling technical field especially relates to a culture apparatus suitable for perennial forest normal position rhizosphere soil sampling.

Background

Rhizosphere generally refers to micro-regional soil with root-soil interface less than a few millimeters, and is also the locus where plants, soil and microorganisms interact with their environmental conditions. The size of the rhizosphere range depends mainly on the root hair length. Due to the action of root hair and root exudates and strong microbial activity in the rhizosphere range, the characteristics of rhizosphere soil in physical, chemical and biological aspects are obviously different from soil body soil.

In the aspect of forestry research, because the forest root system is huge, wide in distribution range and deep, the main root grows vertically downwards, and the lateral roots extend horizontally or obliquely. In the past, the rhizosphere effect of seedlings is researched by a root box method, so that the limitation exists, and the actual condition of the rhizosphere effect of forest trees cannot be truly reflected. In field operation, the rhizosphere sampling mode commonly used still adopts extensive shake-off collection or adopts destructive sampling, namely, after digging out the plant root system, scrapes the soil on the root surface layer and takes it as rhizosphere soil. This method is not accurate enough in distinguishing rhizosphere soil from non-rhizosphere soil, the sampling range is difficult to control, and part of fine roots are easy to fall off and remain in the soil, thereby affecting the test result. In addition, the shaking-off method is also affected by soil humidity, and when the soil humidity is too high or is dry, the soil attached to the root surface is not easy to shake off, which is time-consuming and inaccurate. Therefore, the lack of suitable research methods and means becomes a bottleneck limiting the nutrient conversion and microbial characteristic research of the rhizosphere soil micro-domain of the perennial forest trees.

SUMMERY OF THE UTILITY MODEL

Based on this, it is necessary to provide a culture device suitable for sampling the in-situ rhizosphere soil of perennial forest trees.

The utility model provides a culture apparatus suitable for perennial forest normal position rhizosphere soil sample, includes casing, first net piece and two at least second net pieces, be provided with in the casing and gather the chamber, it has the opening to gather the chamber, first net piece set up in the casing just seals the opening, each second net piece interval set up in the casing, the aperture of first net piece is greater than the aperture of second net piece, first net piece and neighbouring second net piece interval sets up, gather the bottom in chamber and neighbouring second net piece interval sets up.

Above-mentioned culture apparatus that is applicable to perennial forest normal position rhizosphere soil sample, first net piece and adjacent second net piece interval set up in order to form the growth room that is used for the root system to grow, and the growth room is used for filling soil, and also can fill required chemical fertilizer, fertilizer etc. of experiment, and the bottom of gathering the chamber sets up in order to form the sampling room that is used for filling soil with adjacent second net piece interval. When the cultivation device is used, the cultivation device is buried in the ground, the cultivation device is obliquely placed in the soil, and the opening is upward. The root system grows over time and extends to the uptake of nutrients within the growth chamber. Furthermore, root exudates and rhizosphere microorganisms generated by the root system can act on the soil in the sampling chamber. During the cultivation process, the root system which is suitable for passing through the aperture of the first net sheet is difficult to enter the second net sheet with smaller aperture, so that the root system can stably grow in the growth chamber. Rhizosphere soil and microorganisms can better pass through the second meshes with smaller apertures and are juxtaposed in the areas between the second meshes to facilitate interaction with the root system. Therefore, after the culture device is dug out, rhizosphere soil samples with a certain distance from the surface of the root system can be obtained between the second meshes and used for analyzing indexes such as nutrient conversion, microbial activity, root system secretion and the like. The culture device suitable for perennial forest in-situ rhizosphere soil sampling can quickly and accurately collect rhizosphere soil and non-rhizosphere soil, cannot damage the growth of root systems in a large area, has the characteristics of simple structure and convenient operation, and is particularly suitable for actual collection work in field fields.

In one embodiment, the first mesh has a pore size of 2 mm.

In one embodiment, the pore size of the second mesh is 30 μm.

In one embodiment, the number of the second meshes is at least three, and the distance between every two second meshes is uniformly arranged.

In one embodiment, the second mesh has a shape that matches the cross-sectional shape of the collection chamber.

In one embodiment, the wall of the collection cavity is provided with at least two annular clamping grooves at intervals, and each second mesh is correspondingly inserted into one annular clamping groove.

In one embodiment, the distance from the bottom of the collection chamber to the adjacent second mesh is 5 cm.

In one embodiment, the housing is provided as a transparent structure.

In one embodiment, the housing includes an upper shell and a lower shell, the first mesh is disposed on the upper shell, the second meshes are disposed on the lower shell, and the upper shell and the lower shell are detachably connected.

Drawings

FIG. 1 is a schematic perspective view of a cultivation apparatus suitable for sampling in-situ rhizosphere soil of perennial forest trees according to an embodiment;

fig. 2 is a schematic perspective view of another embodiment of a culture device suitable for sampling in-situ rhizosphere soil of perennial forest trees.

Detailed Description

In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. The preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

As shown in FIG. 1, in one embodiment, a culture device 10 suitable for in situ rhizosphere soil sampling of perennial forest trees is provided, and for ease of reading, the culture device 10 will be referred to hereinafter. The culture device 10 includes a housing 100, a first mesh 200, and at least two second meshes. A collection chamber is disposed within the housing 100 and has an opening 102. The first mesh 200 is disposed in the housing 100 and closes the opening 102. The second mesh sheets are arranged in the casing 100 at intervals. The first mesh 200 has a larger pore size than the second mesh. The first mesh 200 is spaced from the adjacent second mesh, and the bottom of the collection chamber is spaced from the adjacent second mesh.

In this embodiment, the aperture of the first mesh sheet 200 is 2mm, and the thickness of the first mesh sheet 200 is 2mm, so that the thin root system can better penetrate through the first mesh sheet 200, and the culture device 10 can be prevented from being damaged from the inside due to the thick root entering the casing 100. In this embodiment, first net piece 200 is the nylon nethike embrane to make the root system pass through betterly, the nylon nethike embrane has higher intensity simultaneously, damages first net piece 200 when avoiding root system growth.

In this embodiment, the aperture of the second net piece is 30 μm, the thickness of the second net piece is 90 μm, and the second net piece in this aperture can block the root system better to avoid the root system to pass through the second net piece, nutrients and microorganisms can pass through better simultaneously, so can collect root system soil better. In this example, the second mesh is a polyamide microporous membrane to allow better passage of nutrients and microorganisms.

The current shake-off method can not realize the distinguishing and collecting of soil samples at different distances of the rhizosphere, and further influences the distance gradient effect of the rhizosphere process. In this embodiment, the number of the second mesh sheets is at least three, and the number of the second mesh sheets is respectively an upper layer second mesh sheet 301, a middle second mesh sheet 302, and a lower layer second mesh sheet 303, and the distances between the second mesh sheets are uniformly set. The rhizosphere soil of a part can be accommodated between two liang of second net pieces, and the rhizosphere soil of different distances apart from the root system can be gathered after the interval sets up. In other embodiments, the number of the second net sheets is at least five, and the distance between the adjacent second net sheets is 1mm, so that rhizosphere soil within 1mm from the root system, 1mm to 2mm from the root system, 2mm to 3mm from the root system and 3mm to 4mm from the root system can be respectively collected. The number of the second net sheets can be adjusted according to factors such as the growth condition of the trees, the experiment requirement and the like.

In this embodiment, the second mesh has a shape that matches the cross-sectional shape of the collection chamber. That is, the second mesh completely covers the section of the collection chamber, avoiding the root system to bypass the second mesh and enter the area of the collection rhizosphere.

In this embodiment, the wall portion of the collection cavity is provided with at least two annular clamping grooves at intervals, each second mesh sheet is correspondingly inserted into one annular clamping groove, and the second mesh sheet is flexible and can be deformed to be clamped into the annular clamping grooves. In this embodiment, the second mesh is inserted into the slot and then supported and limited, so that the second mesh is fixed in the collection cavity. In other embodiments, the first and second mesh sheets are adhered to the wall of the collection chamber.

In this embodiment, at least one of a fertilizer, an organic fertilizer, and organic residues is disposed between the first mesh and the adjacent second mesh for cultivating a root system.

In this embodiment, the distance from the bottom of the collection chamber to the adjacent second mesh is 5 cm. This distance is more sufficient to accommodate a more appropriate amount of soil for better microbial proliferation.

In this embodiment, the housing 100 is a transparent structure. This allows the condition of the collection chamber within the housing 100 to be externally observed to facilitate processing of the sample within the collection chamber.

As shown in fig. 2, in the present embodiment, the housing 100 includes an upper housing 110 and a lower housing 120, the first mesh sheet 200 is disposed on the upper housing 110, the second mesh sheets are disposed on the lower housing 120, and the upper housing 110 and the lower housing 120 are detachably connected. In this way, the upper and

lower casings

110 and 120 can be quickly separated to separate the root system from the rhizosphere soil, facilitating subsequent separation of the rhizosphere soil. In this embodiment, the upper case 110 and the lower case 120 are bonded. In this embodiment, the upper case 110 and the lower case 120 are bonded by the adhesive layer 400, specifically, the adhesive layer 400 is disposed on the inner side surface of the upper case 110, the upper case 110 is fitted on the outer side surface of the lower case 120, and the adhesive layer 400 is bonded on the outer side surface of the lower case 120. In one embodiment, the adhesive layer is a double-sided tape. In one embodiment, the adhesive layer is formed by curing glue. In other embodiments, the upper and lower shells are threaded. In other embodiments, the upper and lower shells snap into engagement.

In one embodiment, the material of the housing is polyvinyl chloride. In other embodiments, the material of the housing may be polycarbonate, polyvinyl chloride, polystyrene, or other lightweight materials for portability. In this embodiment, gather the cross section in chamber and personally submit circularly, and the outline of casing is circularly, gathers the diameter 15cm in chamber, can adapt to most root system like this, and the root system of being convenient for grows better in the growth indoor.

Above-mentioned culture apparatus 10 that is applicable to perennial forest in situ rhizosphere soil sampling, first net piece 200 and adjacent second net piece interval set up in order to form the growth room 103 that is used for the root system to grow, and growth room 103 is used for filling soil, and also can fill required chemical fertilizer, fertilizer etc. of experiment, and the bottom in collection chamber sets up in order to form the sampling room 104 that is used for filling soil with adjacent second net piece interval. When the cultivation device is used, the cultivation device is buried in the ground, the cultivation device is obliquely placed in the soil, and the opening is upward. The root system grows over time and extends to the uptake of nutrients within growth chamber 103. Further, root exudates and rhizosphere microorganisms generated from the root system react with the soil in the sampling chamber 104. During the cultivation process, it is difficult for the roots adapted to pass through the apertures of the first mesh 200 to enter the second mesh having smaller apertures, so that the roots stably grow in the growth chamber 103. Rhizosphere soil and microorganisms can better pass through the second meshes with smaller apertures and are juxtaposed in the areas between the second meshes to facilitate interaction with the root system. Thus, after the culture device 10 is dug out, rhizosphere soil samples with a certain distance from the surface of the root system can be obtained between the second meshes and used for analyzing indexes such as nutrient conversion, microbial activity, root system secretion and the like. The culture device 10 suitable for in-situ rhizosphere soil sampling of perennial forest trees can quickly and accurately collect rhizosphere soil and non-rhizosphere soil, cannot damage the growth of root systems in a large area, has the characteristics of simple structure and convenient operation, and is particularly suitable for actual collection work in field fields.

Further, the housing is inclined at an acute angle to the ground to facilitate entry of the root system from the first mesh. Further, the housing is inclined at 45 ° to the ground, facilitating entry of the root system from the first mesh and allowing better retention of rhizosphere soil and microorganisms between the second mesh.

In one embodiment, a plurality of protruding rods extend outwards from the outer side surface of the shell, the protruding rods are different in orientation and are used for being in contact with soil when the culture device is buried underground, and the protruding rods can be inserted into the soil in different directions respectively, so that the soil can provide supporting force in different directions through the shell, the stability between the soil and the shell is increased as much as possible, and the shell is prevented from being displaced due to soil loosening. In one embodiment, the marking rod extends outwards from the outer side surface of the shell and is longer than the protruding rod, and the marking rod is used for being arranged upwards to protrude out of the ground, so that the culture device can be found conveniently after an experiment. According to the requirements of users, the marking rod can be set to be easily identified in color so as to be convenient to find the culture device. In one embodiment, the culture device is provided with a GPS (Global Positioning System) transmitter, so that the user can find the culture device through the GPS receiver, which is beneficial for finding the culture device outdoors.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. The utility model provides a culture apparatus suitable for perennial forest normal position rhizosphere soil sampling, a serial communication port, includes casing, first net piece and two at least second net pieces, be provided with in the casing and gather the chamber, it has the opening to gather the chamber, first net piece set up in casing and seal the opening, each second net piece interval set up in the casing, the aperture of first net piece is greater than the aperture of second net piece, first net piece and neighbouring second net piece interval sets up, the bottom and the neighbouring of gathering the chamber second net piece interval sets up.

2. The culture device suitable for sampling rhizosphere soil in situ of perennial forest trees as recited in claim 1, wherein the first mesh has a pore size of 2 mm.

3. The culture device suitable for sampling rhizosphere soil in situ of perennial forest trees as recited in claim 1, wherein the second mesh has a pore size of 30 μm.

4. The cultivation device suitable for sampling rhizosphere soil in situ of perennial forest trees as claimed in claim 1, wherein the number of the second meshes is at least three, and the distance between every two second meshes is uniformly arranged.

5. The culture device suitable for in situ rhizosphere soil sampling of perennial forest trees as recited in claim 1, wherein the second mesh is shaped to match the cross-sectional shape of the collection chamber.

6. The cultivation device suitable for sampling the in-situ rhizosphere soil of perennial forest trees as claimed in claim 1, wherein the wall of the collection cavity is provided with at least two annular clamping grooves at intervals, and each second net piece is correspondingly inserted into one annular clamping groove.

7. The cultivation device for in-situ rhizosphere soil sampling of perennial forest trees as recited in claim 1, wherein the distance from the bottom of the collection chamber to the adjacent second mesh is 5 cm.

8. The cultivation device for sampling rhizosphere soil in situ of perennial forest trees as claimed in claim 1, wherein the housing is provided in a transparent structure.

9. The cultivation device suitable for in-situ rhizosphere soil sampling of perennial forest trees as claimed in claim 1, wherein the housing comprises an upper shell and a lower shell, the first net piece is arranged on the upper shell, each second net piece is arranged on the lower shell, and the upper shell and the lower shell are detachably connected.