CN203909038U - Multipath olfactometer - Google Patents
Multipath olfactometer Download PDFInfo
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- CN203909038U CN203909038U CN201420309177.5U CN201420309177U CN203909038U CN 203909038 U CN203909038 U CN 203909038U CN 201420309177 U CN201420309177 U CN 201420309177U CN 203909038 U CN203909038 U CN 203909038U
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- olfactometer
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Abstract
The utility model discloses a multipath olfactometer belonging to the field of agricultural experiment equipment. The multipath olfactometer comprises four straight chambers, four connecting channels and a worm receiving chamber, wherein the four straight chambers are distributed at the periphery of the worm receiving chamber, each straight chamber is communicated with the worm receiving chamber through one connecting channel, and is provided with a worm receiving opening. According to the multipath olfactometer, the worm receiving chamber is communicated with the straight chambers through multiple connecting channels to form multiple paths. On the premise of not affecting the operating complexity, the multipath olfactometer can simultaneously determine the behavioral effects of various aromatic plants on aphis pomi and harmonia axyridis which is the natural enemy of the aphis pomi, is high in simulative authenticity and improves the experimental efficiency.
Description
Technical field
The utility model relates to the experimental facilities field of fruit tree research, particularly relates to a kind of multi-path olfactometer.
Background technology
Existing in the research of fruit tree, often need to study the behavioral implications of fragrant plant to apple yellow aphid and natural enemy harmonia axyridia thereof.Many employing field inspections or utilization at present only has the olfactometer of a path to observe.But be limited to the test condition of olfactometer, can only test the behavioral implications of a kind of fragrant plant to apple yellow aphid and natural enemy harmonia axyridia thereof at every turn, not only testing efficiency is low,, there is the problem that Reality simulation is low and testing efficiency is low in situation about also cannot the multiple fragrant plant of real simulation nature coexisting.
Utility model content
The technical problems to be solved in the utility model is to provide a kind of multi-path olfactometer, can facilitate and measure the behavioral implications of multiple fragrant plant to apple yellow aphid and natural enemy harmonia axyridia thereof simultaneously, and Reality simulation is high, improves conventional efficient.
For solving the problems of the technologies described above, the utility model provides a kind of multi-path olfactometer, comprising:
Four straight chambers, four interface channels and one connect zooecium; Wherein,
Described in being arranged on, described four straight chambers distributions connect zooecium periphery;
Described each straight chamber by a described interface channel with described in connect zooecium and be communicated with;
The described zooecium that connects is provided with and connects insect population.
The beneficial effects of the utility model are: utilize multiple interface channels will connect zooecium and be communicated with multiple straight chambers, form many paths.Do not affecting under the prerequisite of Operating Complexity, can measure the behavioral implications of multiple fragrant plant to apple yellow aphid and natural enemy harmonia axyridia thereof simultaneously, Reality simulation is high, improves conventional efficient.
Brief description of the drawings
In order to be illustrated more clearly in the technical scheme of the utility model embodiment, below the accompanying drawing of required use during embodiment is described is briefly described, apparently, accompanying drawing in the following describes is only embodiment more of the present utility model, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain other accompanying drawings according to these accompanying drawings.
The schematic top plan view of the multi-path olfactometer that Fig. 1 provides for the utility model embodiment.
The part schematic side view of the multi-path olfactometer that Fig. 2 provides for the utility model embodiment.
Embodiment
Below the technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiment.Based on embodiment of the present utility model, those of ordinary skill in the art, not making the every other embodiment obtaining under creative work prerequisite, belong to protection domain of the present utility model.
Fig. 1,2 is depicted as a kind of multi-path olfactometer that the utility model embodiment provides, and can be used for testing the behavior of fruit tree insect, and this multi-path olfactometer comprises: four straight chambers 1,11,12,13, four interface channels 2,21,22,23 and one connect zooecium 4;
Wherein, four straight chambers 1,11,12,13 distribute to be arranged on and connect zooecium 4 peripheries;
Each straight chamber by an interface channel with connect zooecium 4 and be communicated with;
Connecing zooecium 4 is provided with and connects insect population 3.
In above-mentioned multi-path olfactometer, straight chamber is the rectangular parallelepiped that transparent material (as being polythene material or glass) is made, and upper end, straight chamber is provided with opening, and opening is provided with sealing gauze layer.Concrete, the length × wide × height of straight chamber is 80cm × 80cm × 140cm.
In above-mentioned multi-path olfactometer, interface channel is that transparent material (as being polythene material or the glass) two ends of making are provided with the rectangular parallelepiped of opening.Concrete, the length × wide × height of interface channel is 15cm × 10cm × 10cm.
In above-mentioned multi-path olfactometer, connecing zooecium is the square that transparent material (as being polythene material or glass) is made, on its end face, arrange and connect insect population, four sidewalls respectively arrange an interface mating with the opening of interface channel, interface can be arranged on the middle part of sidewall, and its size can be the 10cm × 10cm of coupling interface channel opening.Concrete, the length × wide × height that connects zooecium is 15cm × 15cm × 15cm.
In above-mentioned multi-path olfactometer, each straight chamber by an interface channel with connect zooecium and be communicated with and be: the opening that the sidewall setting of straight chamber mate with the opening of interface channel, the directly bottom surface 80cm of chamber of opening distance, is connected with interface channel one end through opening; The interface channel other end with connect zooecium and be connected.
Below in conjunction with concrete use procedure, the multi-path olfactometer of the utility model embodiment is described further.
(1) experiment place: test is carried out in Beijing Agricultural College's controllability growth simulation greenhouse.
(2) test material:
1. fruit tree seedling: select growth potential, plant height (≤1.20m), do the relatively consistent biennial young Fuji apple trees (Malus domestica CV.Fuji/Malus baccata Borkh.) such as thick, being transplanted to diameter is 40cm, in the plastic tub of high 50cm, every basin one strain, normal management.
2. fragrant plant: the fragrant plant that field test is chosen is seeded in diameter 10cm, the plastic tub kind of high 12cm, treats after emerging to carry out thinning about height of seedling 5cm, and every basin stays seedling 4-6 strain, and plant to be planted supplies examination after blooming.
3. apple yellow aphid: sample from the tender tip of experiment orchard apple tree at duration of test.The apple yellow aphid of taking is carried out on apple sapling plant to indoor feeding (Beijing Agricultural College insectary), indoor temperature is (28 ± 5) DEG C, relative humidity 60%-80%, and periodicity of illumination 16L:8D, supplies examination after at least two generations of carrying propagation.
4. harmonia axyridia: adult is trapped in test orchard, takes back laboratory and supplies examination with apple yellow aphid captive breeding, and indoor temperature is (26 ± 3) DEG C, relative humidity 70%-80%, periodicity of illumination 14L:10D.
(3) test design and method:
1. test with potted plant pattern and carry out, utilize the multi-path olfactometer (as shown in Figure 1, 2) of the utility model embodiment to measure the behavioral implications of fragrant plant to apple yellow aphid and natural enemy harmonia axyridia thereof.Arrange apple tree separately potted plant+inoculation aphid (T1), apple tree seedling+fragrant plant+inoculation aphid (T2), apple tree separately potted plant+do not inoculate aphid (T3), apple tree seedling+fragrant plant+do not inoculate aphid (T4) totally four processing (apple seedling+fragrant plant is directly put in four basin fragrant plants in fruit tree seedling basin while combination, inoculation Aphed population is 100 heads/plant), four repetitions are established in test.When experiment, each processing plant is positioned over respectively in four straight chambers of rectangular parallelepiped of multi-path olfactometer, then seals the top ports of four straight chambers of rectangular parallelepiped with gauze.Connect respectively again four interface channels and middle square connects zooecium, connect zooecium and connect insect population and seal with gauze equally.Record 1 day, 2 days, 4 days, 8 days, 16 days Aphed populations, simultaneously before inoculation aphid and inoculation aphid take food after 12 hours, take respectively fragrant plant, the aphid fruit tree leaf before and after taking food to carry out volatile matter mensuration.Volatile matter composition measurement adopts headspace solid-phase microextraction-By Gas Chromatography-mass Spectrometry.Four groups of same treatment are set in addition, inoculation aphid is after 12 hours, connect insect population at indirect zooecium and put into 40 hungry Imagoes of Harmonia Axyridis of processing 12 hours, then seal and connect insect population with gauze, observe 6 hours, 12 hours, 2 days, 4 days, 8 days harmonia axyridia distributions, quantity and predations.
2. the above-mentioned aromatic plant volatility material that records is carried out after qualitative and quantitative analysis, determine main performance property active component, carry out active component effect mensuration.Buy purity more than 98% or the main performance material of AR level, compound concentration is 0 μ l/L (CK), 25 μ l/L, 50 μ l/L and 100 μ l/L monocyte sample concentration solution and identical four the concentration solution for later use of all main active substances 1:1 potpourris respectively, adopts multi-path olfactometer to measure respectively the impact of each main active on apple yellow aphid survival and the behavior of natural enemy harmonia axyridia.Four repetitions are set respectively when each active substance sample determination.First one of the straight indoor apple tree seedling of putting into respectively 100 apple aphids of inoculation of four of multi-path olfactometers, then eachly straight indoorly respectively put into one of a cotton balls soaking with this sample variable concentrations solution respectively (cotton balls 5ml respective concentration solution soaks, be placed in diameter 10cm double dish), then seal the top ports of four straight chambers of rectangular parallelepiped with gauze.The square that connects respectively again four interface channels and centre connects zooecium, the Aphed population of observed and recorded after 1 hour, 2 hours, 4 hours, 8 hours, 1 day.Four groups of same treatment are set equally, after inoculation aphid, connect insect population at indirect zooecium and put into 40 hungry Imagoes of Harmonia Axyridis of processing 12 hours, then seal and connect insect population with gauze, observe harmonia axyridia distribution, quantity and predation after 1 hour, 2 hours, 4 hours, 8 hours, 1 day.
In sum, the multi-path olfactometer of the utility model embodiment, simple in structure, can facilitate and measure the behavioral implications of multiple fragrant plant to apple yellow aphid and natural enemy harmonia axyridia thereof simultaneously, Reality simulation is high, improves conventional efficient.
The above; it is only preferably embodiment of the utility model; but protection domain of the present utility model is not limited to this; any be familiar with those skilled in the art the utility model disclose technical scope in; the variation that can expect easily or replacement, within all should being encompassed in protection domain of the present utility model.Therefore, protection domain of the present utility model should be as the criterion with the protection domain of claims.
Claims (8)
1. a multi-path olfactometer, is characterized in that, comprising:
Four straight chambers, four interface channels and one connect zooecium; Wherein,
Described in being arranged on, described four straight chambers distributions connect zooecium periphery;
Described each straight chamber by a described interface channel with described in connect zooecium and be communicated with;
The described zooecium that connects is provided with and connects insect population.
2. multi-path olfactometer according to claim 1, is characterized in that, described straight chamber is the rectangular parallelepiped that transparent material is made, and upper end, straight chamber is provided with opening, and described opening is provided with sealing gauze layer.
3. multi-path olfactometer according to claim 2, is characterized in that, the length × wide × height of described straight chamber is 80cm × 80cm × 140cm.
4. multi-path olfactometer according to claim 1, is characterized in that, described interface channel is the rectangular parallelepiped that two ends that transparent material is made are provided with opening.
5. multi-path olfactometer according to claim 4, is characterized in that, the length × wide × height of described interface channel is 15cm × 10cm × 10cm.
6. multi-path olfactometer according to claim 1, is characterized in that, described in to connect zooecium be the square that transparent material is made, described in arranging on its end face, connect insect population, four sidewalls respectively arrange an interface mating with the opening of interface channel.
7. multi-path olfactometer according to claim 1, is characterized in that, described in to connect the length of zooecium × wide × high be 15cm × 15cm × 15cm.
8. according to the multi-path olfactometer described in claim 1 to 7 any one, it is characterized in that, described each straight chamber by a described interface channel with described in connect zooecium and be communicated with and be:
The opening that the sidewall setting of described straight chamber is mated with the opening of described interface channel, described opening, apart from the bottom surface 80cm of described straight chamber, is connected with interface channel one end through described opening;
The described interface channel other end with described in connect zooecium and be connected.
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| CN201420309177.5U CN203909038U (en) | 2014-06-11 | 2014-06-11 | Multipath olfactometer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103995101A (en) * | 2014-06-11 | 2014-08-20 | 北京农学院 | Multi-channel olfactometer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103995101A (en) * | 2014-06-11 | 2014-08-20 | 北京农学院 | Multi-channel olfactometer |
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