CN101693870A - Culture method and device of plant rhizosphere soil microorganism - Google Patents
Culture method and device of plant rhizosphere soil microorganism Download PDFInfo
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- CN101693870A CN101693870A CN 200910070778 CN200910070778A CN101693870A CN 101693870 A CN101693870 A CN 101693870A CN 200910070778 CN200910070778 CN 200910070778 CN 200910070778 A CN200910070778 A CN 200910070778A CN 101693870 A CN101693870 A CN 101693870A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/34—Internal compartments or partitions
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Abstract
The invention relates to culture method and device of plant rhizosphere soil microorganism. The culture device is a cuboid-shaped container, and the cuboid-shaped container is divided into three parts by two pieces of nylon net, wherein two sides are plant growing chambers, and the middle part is a soil chamber, and the aperture of each piece of nylon net is 30 micrometers; and the culture device is made of non-transparent organic glass plates, or hard plastic plats or PVC plates. The culture method of plant rhizosphere soil microorganism comprises two steps of culturing and sampling. The soil chamber is separated from the plant growing chambers by adopting the two pieces of nylon net to prevent root systems from passing through the plant growing chambers to reach the soil chamber, thereby conveniently separating the plant root systems from the rhizosphere soil; the method has the advantages of convenient sampling, accurate detection and reasonable design.
Description
Technical field
The present invention relates to a kind of soil microorganisms cultural method and culture apparatus, be specifically related to a kind of plant rhizosphere soil method for culturing microbes and culture apparatus.
Background technology
The German microbiologist Hiltner of rhizosphere (Rhizosphere) at first put forward in 1904, and was defined as that a part of soil microcell that microbial population is subjected to the root system influence, and scope is generally from root table 1～5mm.Rhizospheric microorganism is bacterium, actinomycetes, the fungi that is present in root surface and its surrounding soil, algae, protozoon and virus etc.The different growing of different plants even same plant, its root exudates all can exert an influence to growing of rhizospheric microorganism.And rhizospheric microorganism participates in multiple soil physiological and biochemical procedures such as the conversion of formation, nutrient of decomposition, the soil ulmin of the soil organism and circulation.Therefore, analyze and compare the influence of plant, can judge and estimate the influence of plant the soil microenvironment to rhizospheric microorganism.
A large amount of microorganisms normally grows in the unsettled running balance in the soil, and root system of plant is to cause soil microorganisms to produce important factor jumpy.Therefore rhizospheric microorganism research also receives much attention.Since the seventies, quite active about the research of rhizospheric microorganism in the world, the chemical process of root pedosphere face has become the research focus.But for the sampling method of rhizosphere soil, the tradition research artificially shake partition methods that adopt more.There is the inhomogenous defective of sampling amount etc. in this method under conditions such as different floristics, soil type and field humidity, and easily cause the minority root system to organize the residual of root hair especially, measure thereby influence the rhizosphere soil microorganism quantitative analysis, influenced the accuracy of test.
Summary of the invention
The present invention has overcome the deficiencies in the prior art, and a kind of science, reasonable is provided, and measures a kind of accurately plant rhizosphere soil method for culturing microbes and corresponding culture apparatus.
For solving above-mentioned technical problem, the present invention by the following technical solutions:
The culture apparatus of a kind of plant rhizosphere soil microorganism, described culture apparatus is a cuboid container, described cuboid container is divided into three parts by two nylon wires, wherein both sides are phytotron, the centre is the soil chamber, the aperture of described nylon wire is 30 μ m, and described culture apparatus adopts nontransparent poly (methyl methacrylate) plate or hard plastic board or PVC plate.
A kind of plant rhizosphere soil method for culturing microbes comprises the steps:
One, cultivate: the Soil and Fertilizer mixing is crossed the 1mm sieve, be respectively charged into phytotron and soil chamber in the described culture apparatus of claim 1, soil is apart from culture apparatus edge 1.5cm, watering then and making the interior weight water content of entire container is 16%～20%, plant seed is carried out surface sterilization with 10% clorox, use deionized water rinsing then 3 times, seed was put under 25 ℃ of dark conditions of culture dish vernalization 1～2 day, be seeded in phytotron at last, in growing process, utilize weighting method to replenish phytotron and soil chamber moisture, make the interior weight water content of entire container remain on 16%～20%.
Two, sampling: when cultivating for 6～8 weeks, with soil separately, keep the indoor undisturbed soil of soil at the nylon wire place, and according to distance phytotron 0～5mm soil sample, this part soil is rhizosphere soil, and the earth sample that fetches earth in this way can carry out the index determining of soil microorganisms.
Among the present invention, the use of nylon wire can stop root system to pass phytotron arrival soil chamber, can easily root system of plant and root soil be separated, and sampling is convenient, and measures accurately, and is reasonable in design.
Description of drawings
Fig. 1 is the structural representation of culture apparatus of the present invention.
Embodiment
The present invention is further elaborated below in conjunction with accompanying drawing.
As shown in Figure 1, the culture apparatus of a kind of plant rhizosphere soil microorganism, described culture apparatus is a cuboid container, described cuboid container is divided into three parts by two nylon wires 3, wherein both sides are phytotron 1,1 ', the centre is soil chamber 2, and the aperture of described nylon wire is 30 μ m, and described culture apparatus adopts nontransparent poly (methyl methacrylate) plate or hard plastic board or PVC plate.
A kind of plant rhizosphere soil method for culturing microbes comprises the steps:
One, cultivate: the Soil and Fertilizer mixing is crossed the 1mm sieve, be respectively charged into the phytotron 1 in the described culture apparatus of claim 1,1 ' and soil chamber 2, soil is apart from culture apparatus edge 1.5cm, watering then and making the interior weight water content of entire container is 16%～20%, plant seed is carried out surface sterilization with 10% clorox, use deionized water rinsing then 3 times, seed was put under 25 ℃ of dark conditions of culture dish vernalization 1～2 day, be seeded in phytotron at last, in growing process, utilize weighting method to replenish phytotron and soil chamber moisture, make the interior weight water content of entire container remain on 16%～20%.
Two, sampling: when cultivating for 6～8 weeks, with soil separately, keep the indoor undisturbed soil of soil at nylon wire 3 places, and according to distance phytotron 0～5mm soil sample, this part soil is rhizosphere soil, and the earth sample that fetches earth in this way can carry out the index determining of soil microorganisms.
Claims (2)
1. the culture apparatus of a plant rhizosphere soil microorganism, described culture apparatus is a cuboid container, it is characterized in that: described cuboid container is divided into three parts by two nylon wires, wherein both sides are phytotron, the centre is the soil chamber, the aperture of described nylon wire is 30 μ m, and described culture apparatus adopts nontransparent poly (methyl methacrylate) plate or hard plastic board or PVC plate.
2. a plant rhizosphere soil method for culturing microbes is characterized in that comprising the steps:
One, cultivate: the Soil and Fertilizer mixing is crossed the 1mm sieve, be respectively charged into phytotron and soil chamber in the described culture apparatus of claim 1, soil is apart from culture apparatus edge 1.5cm, watering then and making the interior weight water content of entire container is 16%～20%, plant seed is carried out surface sterilization with 10% clorox, use deionized water rinsing then 3 times, seed was put under 25 ℃ of dark conditions of culture dish vernalization 1～2 day, be seeded in phytotron at last, in growing process, utilize weighting method to replenish phytotron and soil chamber moisture, make the interior weight water content of entire container remain on 16%～20%;
Two, sampling: when cultivating for 6～8 weeks, with soil separately, keep the indoor undisturbed soil of soil at the nylon wire place, and according to distance phytotron 0～5mm soil sample, this part soil is rhizosphere soil, and the earth sample that fetches earth in this way can carry out the index determining of soil microorganisms.
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| CN 200910070778 CN101693870A (en) | 2009-10-13 | 2009-10-13 | Culture method and device of plant rhizosphere soil microorganism |
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| CN 200910070778 CN101693870A (en) | 2009-10-13 | 2009-10-13 | Culture method and device of plant rhizosphere soil microorganism |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283037A (en) * | 2011-05-17 | 2011-12-21 | 中国科学院东北地理与农业生态研究所 | Simulative culture device and method for crop rhizosphere deposit carbon |
| CN103235102A (en) * | 2013-04-09 | 2013-08-07 | 广西壮族自治区中国科学院广西植物研究所 | Method and device for determining plant root system and hyphae respiration rates |
| CN104789464A (en) * | 2015-04-30 | 2015-07-22 | 环境保护部南京环境科学研究所 | In-situ sampling device and sampling method for aquatic plant root rhizospheric microorganism in different parts |
| CN105319261A (en) * | 2015-11-17 | 2016-02-10 | 浙江大学 | Device and method for determining soil mass flow and diffusion property |
| CN108845090A (en) * | 2018-06-01 | 2018-11-20 | 重庆工业职业技术学院 | A method of probing into reservoir drawdown band typical vegetation rhizosphere micro-region mercury activating effect |
| CN114134020A (en) * | 2021-12-01 | 2022-03-04 | 北京林业大学 | Nondestructive sampling device and nondestructive sampling method for plant rhizosphere microorganisms |
| CN114946473A (en) * | 2022-04-13 | 2022-08-30 | 同济大学 | Multi-interlayer plant incubator for researching rhizosphere microecological characteristics and application method thereof |
-
2009
- 2009-10-13 CN CN 200910070778 patent/CN101693870A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283037A (en) * | 2011-05-17 | 2011-12-21 | 中国科学院东北地理与农业生态研究所 | Simulative culture device and method for crop rhizosphere deposit carbon |
| CN102283037B (en) * | 2011-05-17 | 2012-12-26 | 中国科学院东北地理与农业生态研究所 | Simulative culture device and method for crop rhizosphere deposit carbon |
| CN103235102A (en) * | 2013-04-09 | 2013-08-07 | 广西壮族自治区中国科学院广西植物研究所 | Method and device for determining plant root system and hyphae respiration rates |
| CN104789464A (en) * | 2015-04-30 | 2015-07-22 | 环境保护部南京环境科学研究所 | In-situ sampling device and sampling method for aquatic plant root rhizospheric microorganism in different parts |
| CN104789464B (en) * | 2015-04-30 | 2017-01-18 | 环境保护部南京环境科学研究所 | In-situ sampling device and sampling method for aquatic plant root rhizospheric microorganism in different parts |
| CN105319261A (en) * | 2015-11-17 | 2016-02-10 | 浙江大学 | Device and method for determining soil mass flow and diffusion property |
| CN105319261B (en) * | 2015-11-17 | 2017-12-15 | 浙江大学 | Determine soil mass flow and the apparatus and method of diffusion property |
| CN108845090A (en) * | 2018-06-01 | 2018-11-20 | 重庆工业职业技术学院 | A method of probing into reservoir drawdown band typical vegetation rhizosphere micro-region mercury activating effect |
| CN114134020A (en) * | 2021-12-01 | 2022-03-04 | 北京林业大学 | Nondestructive sampling device and nondestructive sampling method for plant rhizosphere microorganisms |
| CN114946473A (en) * | 2022-04-13 | 2022-08-30 | 同济大学 | Multi-interlayer plant incubator for researching rhizosphere microecological characteristics and application method thereof |
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