CN103235102A - Method and device for determining plant root system and hyphae respiration rates - Google Patents
Method and device for determining plant root system and hyphae respiration rates Download PDFInfo
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- CN103235102A CN103235102A CN2013101207368A CN201310120736A CN103235102A CN 103235102 A CN103235102 A CN 103235102A CN 2013101207368 A CN2013101207368 A CN 2013101207368A CN 201310120736 A CN201310120736 A CN 201310120736A CN 103235102 A CN103235102 A CN 103235102A
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Abstract
The invention discloses a method and a device for determining plant root system and hyphae respiration rates. The device comprises a root system chamber, a hyphae chamber, a soil chamber, and an interference-free control room, which are buried underground. The method comprises the steps that: within a test area, a plant is selected; at least four pits are dug around a plant; the chambers are respectively placed into the pits, wherein the tops of the containers forming the chambers are aligned with the ground level or slightly exposed when the chambers are placed into the pits, and the dug soil is respectively refilled into the chambers; after certain time of burying, respiration rates in the root system chamber, the hyphae chamber, the soil chamber, and the interference-free control room are respectively determined; the ratio of respirations of plant root system, hyphae, and other soil microbes in the root system chamber is calculated according to a certain formula; and the respiration rates of plant root system, hyphae, and other soil microbes are calculated according to the formula. With the method and device provided by the invention, pure root respiration and external hyphae respiration can be separated from soil respiration.
Description
Technical field
The present invention relates to the pedology studying technological domain, be specifically related to measure the method and apparatus of root system of plant and mycelia respiratory rate.
Background technology
Soil is the important component part of terrestrial ecosystems, is one of carbon storehouse of earth maximum.Soil respiration is that terrestrial ecosystems discharge CO to atmosphere
2One of main path.As everyone knows, CO in the atmosphere
2Only pass through that single channel---photosynthesis enters into terrestrial ecosystems, yet, CO in the ecosystem
2Then turn back to atmosphere by a plurality of processes.Wherein, ecosystem breathing is main path; Soil respiration then is the pith that the ecosystem is breathed, and be input to Atmospheric CO by soil respiration every year
2Amount just account for Atmospheric CO
220～40% of general input.Mycorhiza is the commensal of root system of plant and soil fungi, and the land has mycorhiza above 90% higher plant, and the outside mycelia network of mycorhiza is very abundant, and the biomass of mycelia and respiration are converged to soil carbon and the soil carbon circulation has material impact.Discover that the carbon significant proportion that is stored in the forest soil is to work by the root mycorrhizal fungi relevant with them.Carbon in the soil has 50%～70%, the fungi of growth around reaching from tree root.Therefore, the very small variation of soil respiration will cause Atmospheric CO
2Remarkable increase or the reduction of concentration, thus have influence on Global climate change.
Though being studied personnel very early to the influence of soil respiration, mycorhiza recognizes, but existing mensuration root system of plant breath method is as " in-situ method ", " Qie Genfa " and " girdling method " etc., though can measure under the field condition respiratory rate of root system of plant in the soil, but can't separate the pure root system respiration of plant and the outer mycelia breathing of root, can't understand mycelia and breathe at the contribution in soil respiration.
Summary of the invention
The technical matters that the present invention will separate rhymed formula provides the mensuration root system of plant that the breathing of pure root system respiration and the outer mycelia of root can be separated and the method and apparatus of mycelia respiratory rate from soil respiration.
The device of mensuration root system of plant of the present invention and mycelia respiratory rate is characterized in that: comprise buried root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber, wherein:
Described root system chamber comprises the container of a vertical placement and top opening, offers aperture at the wall of a container face, and the outside wall surface of described container is provided with one deck stainless steel mesh; Wherein, the aperture of aperture is 2～4cm on the described container, and the centre distance of adjacent two apertures is 4～7cm; The aperture of described stainless steel mesh is for being less than or equal to 2mm;
Described mycelia chamber comprises the container of a vertical placement and top opening, offers aperture at the wall of a container face, and the outside wall surface of described container is bonded with one deck nylon screen, also is provided with one deck stainless steel mesh on the nylon screen of container outer wall face; Wherein, the aperture of aperture is 2～4cm on the described container, and the centre distance of adjacent two apertures is 4～7cm; The aperture of described nylon screen is 20～45 μ m; The aperture of described stainless steel mesh is for being less than or equal to 2mm;
Described soil chamber comprises the container of a vertical placement and top opening, offers aperture at the wall of a container face, and the outside wall surface of described container is bonded with one deck nylon screen, also is provided with one deck stainless steel mesh on the nylon screen of container outer wall face; Wherein, the aperture of aperture is 2～4cm on the described container, and the centre distance of adjacent two apertures is 4～7cm; The aperture of described nylon screen is for being less than or equal to 5 μ m; The aperture of described stainless steel mesh is for being less than or equal to 2mm;
Described noiseless contrast chamber is the container of a vertical placement and the equal opening of Fang Fang and bottom;
Volume of a container and the shape of described formation root system chamber, mycelia chamber and soil chamber are identical; The shape of container that constitutes noiseless contrast chamber is identical with the shape of the container that constitutes root system chamber, mycelia chamber and soil chamber.
In the technique scheme:
Described container can be cylindricality or square other shape that waits, and is preferably cylindrical.The height of the container of described formation root system chamber, mycelia chamber and soil chamber can determine that preferred heights is 20～40cm according to mycorhiza, the degree of depth of mycelia under the distribution face of land; As for the bore of container should not be too greatly also unsuitable too small (the container bore is excessive, can produce big disturbance to the surrounding soil environment, put it into underground after, mycelia on every side needs the long time just can grow in the device; Container is too small, and when measuring respiratory rate, the outside soil gas of device may interfere with measures degree of accuracy), the scope of choosing of bore is 15～25cm usually; The height that constitutes the container of noiseless contrast chamber is 4～1Ocm, and bore is generally 15～25cm.Described container can be to adopt Polyvinylchloride (PVC) material, metal material, foam or other material to make container.
The aperture of aperture is preferably 3cm on the container of described formation root system chamber, mycelia chamber and soil chamber.
The aperture of the stainless steel mesh of described formation root system chamber, mycelia chamber and soil chamber is 1.5～2mm.
The aperture of the nylon screen of described formation mycelia chamber is preferably 30～4O μ m.
The aperture of the nylon screen of described formation soil chamber is preferably 1～3 μ m.
Utilize said apparatus to measure the method for root system of plant and mycelia respiratory rate, may further comprise the steps:
1) in the test site, choose a plant, dig out at least four holes at the plant periphery, the size in described these holes matches with the size of root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber respectively, when described root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber being put into each hole, the top of the container of formation root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber flushes with ground or bassets a little, and the soil that digs out is inserted root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber respectively;
2) after underground certain hour is imbedded in above-mentioned each chamber, measure the respiratory rate of root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber respectively, use the root system chamber
RThe root system chamber respiratory rate that expression records, the mycelia chamber
RThe mycelia chamber respiratory rate that expression records, the soil chamber
RThe soil chamber respiratory rate that expression records, R
AlwaysThe respiratory rate of the noiseless contrast chamber that expression records calculates the ratio of other microbial respiratory of root system of plant, mycelia and soil in breathe the root system chamber according to following formula then;
Wherein, r
RootThe ratio of expression root system respiration in breathe the root system chamber, r
BacteriumThe ratio of expression mycelia in breathe the root system chamber, r
SoilThe ratio of other microorganism of expression soil in breathe the root system chamber;
3) calculate other microbial respiratory speed of root system of plant, mycelia and soil according to following formula again;
Wherein, R
RootBe pure root system respiration speed, R
BacteriumBe mycelia respiratory rate, R
SoilBe other microbial respiratory speed of soil.
In the said method,
In the step 1), described plant periphery refers to that centered by the trunk of plant radius is in the round scope that covers of 3～5m.
In the step 1), when described root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber being put into each hole, the part that the container of formation root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber bassets is less than or equal to 3cm, and 2～3cm preferably bassets.
Step 2) in, measure above-mentioned root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber respiratory rate time normally around mycelial growth be full of each and measure when indoor, need the time more than 4 months under one situation, normally after each chamber is imbedded underground 4～6 months, the respiratory rate of each chamber is measured.Adopting the soil respiration measuring system to measure when measuring the breathing of each chamber, specifically can be to adopt Li-6400-O9CO
2Analysis cell (LI-COR company, United States Nebraska Lincoln city), the automatic soil CO2 of B-LI-8100 flux system (LI-COR company), the C-soil respiration (PPSYSTEMS of system, Massachusetts, United States Amesbury city) or E-SRC series portable soil respiration system (DYNAMAX company, Houston, TX, USA city) etc. measure.
Compared with prior art, device of the present invention comprises root system chamber, mycelia chamber, soil chamber and noiseless contrast chamber, after adopting the method for the invention that they are imbedded underground certain hour respectively, other microorganism passes in feasible root system chamber permission plant radicula, mycelia and the soil wherein, and the respiration of mensuration has comprised that other composition is breathed in root system respiration, mycelia breathing and the soil; Other microorganism passes in mycelia chamber permission mycelia and the soil, and measuring respiration is other microbial respiratory in mycelia breathing and the soil; Entering of root system of plant and mycelia limited in the soil chamber, and other microorganism in the soil is only arranged, and the respiration that records is other microbial respiratory in the soil; According to the above-mentioned breathing that records, calculate in conjunction with concrete formula again and can draw the ratio that root system respiration and mycelia are breathed in the soil respiration; And be total breathing under the soil natural state by the breathing that noiseless contrast chamber records, with soil total breathe and soil in each component proportion multiply each other and can draw the respiratory rate of other microorganism in root system, mycelia and the soil.
Description of drawings
Fig. 1 is the structural representation of root system chamber in the device of the present invention;
Fig. 2 is the structural representation of mycelia chamber in the device of the present invention;
Fig. 3 is the structural representation of soil chamber in the device of the present invention;
Fig. 4 is the structural representation of noiseless contrast chamber in the device of the present invention;
Fig. 5 is the structural representation that constitutes the container of root system chamber, mycelia chamber and soil chamber in the device of the present invention;
Fig. 6 is the structural representation that constitutes the nylon screen of mycelia chamber in the device of the present invention;
Fig. 7 is the structural representation that constitutes the stainless steel mesh of root system chamber, mycelia chamber and soil chamber in the device of the present invention.
Number in the figure is:
Root system chamber 1; 2 containers; The 2-1 aperture; 3 stainless steel mesh; 4 nylon screens; Mycelia chambers 5; Soil chambers 6; Noiseless contrast chambers 7.
Embodiment
Embodiment 1: the device that is used for measuring root system of plant and mycelia respiratory rate
Fig. 1～7 are the structural representation of a kind of embodiment of device of the present invention, comprise root system chamber 1, mycelia sky 5, soil chamber 6 and noiseless contrast chamber 7, wherein,
Fig. 1 is the structural representation of root system chamber 1 in this embodiment, it comprises the container 2 of a vertical placement and top opening, wall at container 2 offers aperture 2-1 (as shown in Figure 5), in the outside wall surface of container 2 one deck stainless steel mesh 3 (as shown in Figure 7) is arranged fixedly; The aperture of aperture 2-1 is 2～4cm on the described container 2, and the centre distance between adjacent two aperture 2-1 is 4～7cm; The aperture of described stainless steel mesh 3 is for being less than or equal to 2mm.
Fig. 2 is the structural representation of mycelia chamber 5 in this embodiment, it comprises the container 2 of a vertical placement and top opening, wall at container 2 offers aperture 2-1 (as shown in Figure 5), outside wall surface at container 2 is bonded with one deck nylon screen 4 (as shown in Figure 6), and one deck stainless steel mesh 3 (as shown in Figure 7) is also fixedly arranged on the nylon screen 4 of container 2 outside wall surface; The aperture of aperture 2-1 is 2～4cm on the described container 2, and the centre distance between adjacent two aperture 2-1 is 4～7cm; The aperture of described nylon screen 4 is 20～45 μ m; The aperture of described stainless steel mesh 3 is for being less than or equal to 2mm.
Fig. 3 is the structural representation of soil chamber 6 in this embodiment, it comprises the container 2 of a vertical placement and top opening, wall at container 2 offers aperture 2-1 (as shown in Figure 5), (structure of this nylon screen 4 is identical with the structure of mycelia shown in Figure 2 chamber 5 nylon screens 4 to be bonded with one deck nylon screen 4 in the outside wall surface of container 2, just its aperture is different), on the nylon screen 4 of container 2 outside wall surface, also be provided with one deck stainless steel mesh 3 (as shown in Figure 7); The aperture of aperture 2-1 is 2～4cm on the described container 2, and the centre distance between adjacent two aperture 2-1 is 4～7cm; The aperture of described nylon screen 4 is for being less than or equal to 5 μ m; The aperture of described stainless steel mesh 3 is for being less than or equal to 2mm.
Fig. 4 is the structural representation of noiseless contrast chamber 7 in this embodiment, and it is the container 2 of a vertical placement and top and bottom both ends open.
In the above-described embodiment, the container 2 that constitutes root system chamber 1, mycelia chamber 5 and soil chamber 6 is the cylinder bodily form, they have same height and bore, wherein high is 35cm, diameter is 2Ocm, adopt pvc pipe and PVC plate to make, concrete manufacturing process can be: at first selecting diameter for use is the pvc pipe that the pvc pipe material of 2Ocm is cut into the 35cm height; Get the PVC plate again and cut out the plectane that a diameter is 2Ocm, with polyvinyl chloride adhesive plectane is bonded to an end of cutting out good pvc pipe, as the base plate of container 2; This pvc pipe and plectane constitute the wall of container 2 together, so far then obtain the cylindrical container 2 in the present embodiment.In this embodiment, the aperture of container 2 aperture 2-1 that should guarantee to constitute root system chamber 1, mycelia chamber 5 and soil chamber 6 is consistent with the centre distance of adjacent two apertures.And the container 2 that constitutes noiseless contrast chamber 7 is similarly the cylinder bodily form, and its height is 5cm, and diameter is 2Ocm, is that the pvc pipe that the pvc pipe material of 20cm is cut into the 5cm height gets as long as select diameter during making for use.
In order to obtain comparatively accurate data, described root system chamber 1, mycelia chamber 5, soil chamber 6 and noiseless contrast chamber 7 can make several more, get their mean value then and calculate.
Embodiment 2: the method for measuring root system of plant and mycelia respiratory rate with embodiment 1 described device
1) in the test site, choose a plant, at the plant periphery (centered by the trunk of plant, radius is in the scope that covers of the circle of 3～5m) dig out at least four holes, the size in described these holes respectively with root system chamber 1, mycelia chamber 5, the size of soil chamber 6 and noiseless contrast chamber 7 matches, with described root system chamber 1, mycelia sky 5, when each hole is put in soil chamber 6 and noiseless contrast chamber 7, constitute root system chamber 1, mycelia chamber 5, the top of the container 2 of soil chamber 6 and noiseless contrast chamber 7 flushes with ground or bassets a little (if basset, exposed portions serve is less than or equal to 3cm), and the soil that digs out inserted root system chamber 1 respectively, mycelia chamber 5, soil chamber 6 and noiseless contrast chamber 7;
2) after above-mentioned each chamber is imbedded underground 4～6 months, measure the respiratory rate of root system chamber 1, mycelia chamber 5, soil chamber 6 and noiseless contrast chamber 7 respectively with the soil respiration measuring system, use the root system chamber
RRoot system chamber 1 respiratory rate that expression records, the mycelia chamber
RMycelia chamber 5 respiratory rates that expression records, the soil chamber
RSoil chamber 6 respiratory rates that expression records, R
AlwaysThe respiratory rate of the noiseless contrast chamber 7 that expression records calculates the ratio of other microbial respiratory of root system of plant, mycelia and soil in breathe root system chamber 1 according to following formula then;
Wherein, r
RootThe ratio of expression root system respiration in breathe root system chamber 1, r
RootThe ratio of expression mycelia in breathe root system chamber 1, r
SoilThe ratio of other microorganism of expression soil in breathe root system chamber 1;
3) calculate other microbial respiratory speed of root system of plant, mycelia and soil according to following formula again;
Wherein, R
RootBe pure root system respiration speed, R
BacteriumBe mycelia respiratory rate, R
SoilBe other microbial respiratory speed of soil.
Experimental example
2012～2013 years, utilize apparatus and method of the present invention, in karst ecological experiment station, metropolis township, Guilin city (Guangxi Plant Inst. 2006 set up), root system respiration and mycorhiza mycelia respiratory rate in having measured that soil is total in the oak group of Qinggang and breathing.
1, be used for the making of the device of mensuration root system of plant and mycelia respiratory rate:
Make 3 root system chambers 1 of same specification, 3 mycelia chambers 5 and 3 soil chambers 6 respectively by embodiment 1 described method for making, and make 3 noiseless contrast chambers 7.The container 2 that wherein constitutes root system chamber 1, mycelia chamber 5 and soil chamber 6 is the cylinder bodily form, and their height is 35cm, and diameter is 2Ocm, and the aperture of aperture 2-1 is 3cm on container 2 walls, and the centre distance between adjacent two aperture 2-1 is 5cm.The container 2 that constitutes noiseless contrast chamber 7 is similarly the cylinder bodily form, and its height is 5cm, and diameter is 2Ocm.
2, measure the respiratory rate of root system of plant and mycelia
1) Zhuan Zhi placement:
In August, 2012, in the test site, be the center of circle with the big tree trunk of Qinggang oak, radius is the hole of digging out 9 diameter 2Ocm, dark 35cm in 3～5m scope, described root system chamber 1, mycelia chamber 5 and soil chamber 6 are put into the hole respectively (at this moment, the top that constitutes the container 2 of root system chamber 1, mycelia chamber 5, soil chamber 6 and noiseless contrast chamber 7 flushes with ground), and a soil that digs out is inserted each root system chamber 1, mycelia chamber 5 and soil chamber 6; And then the hole of in above-mentioned scope, digging out 3 diameter 2Ocm, dark 5Cm, hole (at this moment, the top that constitutes the container 2 of noiseless contrast chamber 7 flushes with ground) is put in 3 noiseless contrast chambers 7, and the soil that digs out is inserted each noiseless contrast chamber 7.
2) calculating of other microbial respiratory of root system of plant, mycelia and soil shared ratio in breathe root system chamber 1:
On February 20th, 2013, mornings 9, point was used Li-6400-09C0
2Analysis cell is measured root system chamber 1, mycelia chamber 5, soil chamber 6 and noiseless contrast chamber 7 soil respiration speed respectively.According to 3 replication results, by formula calculate other microorganism of root system of plant, mycelia and soil shared ratio in breathe root system chamber 1.
Record 1 respiratory rate (root system chamber, root system chamber
R) mean value is 1.19 μ molCO
2M
-2S
-1, 5 respiratory rates (mycelia chamber, mycelia chamber
R) mean value is 0.88 μ molCO
2M
-2S
-1, 6 respiratory rates (soil chamber, soil chamber
R) mean value is 0.72 μ molCO
2M
-2S
-1, noiseless contrast chamber 7 respiratory rate (R
Always) mean value is 2.15 μ molCO
2M
-2S
-1With r
RootRepresent the ratio of pure root system respiration in breathe root system chamber 1, with r
BacteriumThe expression mycelia breathes the ratio in breathe root system chamber 1, with r
SoilThe ratio of other microbial respiratory in breathe root system chamber 1 in the expression soil, calculate by following formula:
Draw r as calculated
Root=0.261, r
Bacterium=, 0.134, r
Soil=0.605.
3) calculating of other microbial respiratory respiratory rate of root system of plant, mycelia and soil:
With R
RootRepresent pure root system respiration, with R
BacteriumThe expression mycelia breathes, with R
SoilOther microbial respiratory of expression soil, calculate by following formula:
Draw R as calculated
Root=0.56 μ molCO
2M
-2S
-1, R
Bacterium=0.29 μ molCO
2M
-2S
-1, R
Soil=1.3O μ molCO
2M
-2S
-1, namely in the oakery of Qinggang, pure root system respiration is 056 μ molCO
2M
-2S
-1, mycelia breathes and is 02g μ molCO
2M
-2S
-1, other microbial respiratory of soil is 1.30 μ molCO
2M
-2S
-1
Claims (7)
1. measure the device of root system of plant and mycelia respiratory rate, it is characterized in that: comprise buried root system chamber (1), mycelia chamber (5), soil chamber (6) and noiseless contrast chamber (7), wherein:
Described root system chamber (1) comprises vertical a placement and the container (2) of top opening, offers aperture (2-1) at the wall of container (2), and the outside wall surface of described container (2) is provided with one deck stainless steel mesh (3); Wherein, the aperture that described container (2) is gone up aperture (2-1) is 2～4cm, and the centre distance of adjacent two apertures (2-1) is 4～7cm; The aperture of described stainless steel mesh (3) is for being less than or equal to 2mm;
Described mycelia chamber (5) comprises the container (2) of a vertical placement and top opening, wall at container (2) offers aperture (2-1), the outside wall surface of described container (2) is bonded with one deck nylon screen (4), also is provided with one deck stainless steel mesh (3) on the nylon screen (4) of container (2) outside wall surface; Wherein, the aperture that described container (2) is gone up aperture (2-1) is 2～4cm, and the centre distance of adjacent two apertures (2-1) is 4～7cm; The aperture of described nylon screen (4) is 20～45 μ m; The aperture of described stainless steel mesh (3) is for being less than or equal to 2mm;
Described soil chamber (6) comprises the container (2) of a vertical placement and top opening, wall at container (2) offers aperture (2-1), the outside wall surface of described container (2) is bonded with one deck nylon screen (4), also is provided with one deck stainless steel mesh (3) on the nylon screen (4) of container (2) outside wall surface; Wherein, the aperture that described container (2) is gone up aperture (2-1) is 2～4cm, and the centre distance of adjacent two apertures (2-1) is 4～7cm; The aperture of described nylon screen (4) is for being less than or equal to 5 μ m; The aperture of described stainless steel mesh (3) is for being less than or equal to 2mm;
Described noiseless contrast chamber (7) is the container (2) of a vertical placement and the equal opening in top and bottom;
Volume and the shape of the container (2) of described formation root system chamber (1), mycelia chamber (5) and soil chamber (6) are identical; The shape of container (2) that constitutes noiseless contrast chamber (7) is identical with the shape of the container (2) that constitutes root system chamber (1), mycelia chamber (5) and soil chamber (6).
2. according to claim 1 for the device of measuring root system of plant and mycelia respiratory rate, it is characterized in that: described container (2) is cylindricality or square.
3. according to claim 2 for the device of measuring root system of plant and mycelia respiratory rate, it is characterized in that: the height of the container (2) of described formation root system chamber (1), mycelia chamber (5) and soil chamber (6) is 20～40cm, and bore is 15～25cm; The height that constitutes the container (2) of noiseless contrast chamber (7) is 4～10cm, and bore is 15～25cm.
4. adopt each described device in the claim 1～3 to measure the method for root system of plant and mycelia respiratory rate, may further comprise the steps:
1) in the test site, choose a plant, dig out at least four holes at the plant periphery, the size in described these holes respectively with root system chamber (1), mycelia chamber (5), the size of soil chamber (6) and noiseless contrast chamber (7) matches, with described root system chamber (1), mycelia chamber (5), when each hole is put in soil chamber (6) and noiseless contrast chamber (7), constitute root system chamber (1), mycelia chamber (5), the top of the container (2) of soil chamber (6) and noiseless contrast chamber (7) flushes with ground or bassets a little, and the soil that digs out is inserted root system chamber (1) respectively, mycelia chamber (5), soil chamber (6) and noiseless contrast chamber (7);
2) after underground certain hour is imbedded in above-mentioned each chamber, measure the respiratory rate of root system chamber (1), mycelia chamber (5), soil chamber (6) and noiseless contrast chamber (7) respectively, use the root system chamber
RRoot system chamber (1) respiratory rate that expression records, the mycelia chamber
RMycelia chamber (5) respiratory rate that expression records, the soil chamber
RSoil chamber (6) respiratory rate that expression records, R
AlwaysThe respiratory rate of the noiseless contrast chamber (7) that expression records calculates the ratio of other microbial respiratory of root system of plant, mycelia and soil in breathe root system chamber (1) according to following formula then;
Wherein, r
RootThe ratio of expression root system respiration in breathe root system chamber (1), r
BacteriumThe ratio of expression mycelia in breathe root system chamber (1), r
SoilThe ratio of other microorganism of expression soil in breathe root system chamber (1);
3) calculate other microbial respiratory speed of root system of plant, mycelia and soil according to following formula again;
Wherein, R
RootBe pure root system respiration speed, R
BacteriumBe mycelia respiratory rate, R
SoilBe other microbial respiratory speed of soil.
5. the method for mensuration root system of plant according to claim 4 and mycelia respiratory rate is characterized in that: in the step 1), described plant periphery refers to centered by the trunk of plant, and radius is in the scope of circle of 3～5m.
6. the method for mensuration root system of plant according to claim 4 and mycelia respiratory rate, it is characterized in that: in the step 1), when described root system chamber (1), mycelia chamber (5), soil chamber (6) and noiseless contrast chamber (7) being put into each hole, the part that the container (2) of formation root system chamber (1), mycelia chamber (5), soil chamber (6) and noiseless contrast chamber (7) bassets is less than or equal to 3cm.
7. the method for mensuration root system of plant according to claim 4 and mycelia respiratory rate is characterized in that: step 2) in, be after each chamber is imbedded underground 4～6 months, the respiratory rate of each chamber is measured.
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| CN107646411A (en) * | 2017-10-17 | 2018-02-02 | 沈阳大学 | A kind of plant root growth experimental provision |
| CN108398285A (en) * | 2018-05-25 | 2018-08-14 | 河南大学 | Device and its application method for radicula sampling and fine root production monitoring |
| CN110702857A (en) * | 2019-10-18 | 2020-01-17 | 浙江农林大学 | Method for measuring phyllostachys pubescens rhizome respiration flux |
| CN112014544A (en) * | 2020-09-02 | 2020-12-01 | 复旦大学 | A device for in situ research wetland rhizosphere effect |
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| CN108398285A (en) * | 2018-05-25 | 2018-08-14 | 河南大学 | Device and its application method for radicula sampling and fine root production monitoring |
| CN110702857A (en) * | 2019-10-18 | 2020-01-17 | 浙江农林大学 | Method for measuring phyllostachys pubescens rhizome respiration flux |
| CN112014544A (en) * | 2020-09-02 | 2020-12-01 | 复旦大学 | A device for in situ research wetland rhizosphere effect |
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