CN203191369U - Device for determining respiration of plant root system and mycelia - Google Patents

Device for determining respiration of plant root system and mycelia Download PDF

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Publication number
CN203191369U
CN203191369U CN2013201737444U CN201320173744U CN203191369U CN 203191369 U CN203191369 U CN 203191369U CN 2013201737444 U CN2013201737444 U CN 2013201737444U CN 201320173744 U CN201320173744 U CN 201320173744U CN 203191369 U CN203191369 U CN 203191369U
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container
chamber
aperture
mycelia
root system
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CN2013201737444U
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Chinese (zh)
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张中峰
黄玉清
张春来
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Guangxi Institute of Botany of CAS
Institute of Karst Geology of CAGS
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Guangxi Institute of Botany of CAS
Institute of Karst Geology of CAGS
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Abstract

The utility model discloses a device for determining the respiration of a plant root system and mycelia. The device comprises a root system chamber, a mycelium chamber and a soil chamber, which are buried in the ground and are identical in size and shape, wherein each of the chambers comprises a container which is vertically placed; an opening is formed above the container; small holes are formed in the wall surface of the container; the aperture of each small hole in the container is 2 to 4cm; a central distance between two adjacent small holes is 4 to 7cm; a layer of stainless steel screen is arranged on the outer wall surface of the container of the root system chamber; a layer of nylon screen is adhered to the outer wall surface of the container of the mycelium chamber, and a layer of stainless steel screen is further arranged on the nylon screen on the outer wall surface of the container; a nylon screen is adhered to the outer wall surface of the container of the soil chamber, and a layer of stainless steel screen is arranged on the nylon screen on the outer wall surface of the container. According to the device, the respiration of each chamber can be determined, and the proportion of root system respiration components to mycelium respiration components in soil respiration can be calculated according to a specific formula.

Description

Be used for measuring the device of root system of plant and mycelia breathing
Technical field
The utility model relates to the pedology studying technological domain, is specifically related to for measuring the device that root system of plant and mycelia breathe.
Background technology
Soil is the important component part of terrestrial ecosystems, is one of carbon storehouse of earth maximum.Soil respiration is that terrestrial ecosystems discharge CO to atmosphere 2One of main path.As everyone knows, CO in the atmosphere 2Only pass through that single channel---photosynthesis enters into terrestrial ecosystems, yet, CO in the ecosystem 2Then turn back to atmosphere by a plurality of processes.Wherein, ecosystem breathing is main path; Soil respiration then is the pith that the ecosystem is breathed, and be input to Atmospheric CO by soil respiration every year 2Amount just account for Atmospheric CO 220~40% of general input.Therefore, the very small variation of soil respiration will cause Atmospheric CO 2Remarkable increase or the reduction of concentration, thus have influence on Global climate change.Mycorhiza is the commensal that soil fungi and root system of plant form, and soil respiration is had fundamental influence, is prediction soil CO 2Rate of release must be considered, but the factor that is difficult to estimate.
Though being studied personnel very early to the influence of soil respiration, mycorhiza recognizes, but up to the present, also be difficult to measure pure root system respiration and the breathing of soil mycelia, especially in the open air under the condition, yet there are no more suitable device and method pure root system respiration and mycelia breathing are separated from soil respiration.
The utility model content
The technical problems to be solved in the utility model provides a kind of for measuring the device that root system of plant and mycelia breathe.Utilize this device, in conjunction with concrete computing method, the breathing of pure root system respiration and mycelia can be separated from soil respiration.
Device for mensuration root system of plant and mycelia breathing described in the utility model comprises buried root system chamber, mycelia chamber and soil chamber, wherein:
Described root system chamber comprises the container of a vertical placement and top opening, offers aperture at the wall of a container face, and the outside wall surface of described container is provided with one deck stainless steel mesh; Wherein, the aperture of aperture is 2~4cm on the described container, and the centre distance of adjacent two apertures is 4~7cm; The aperture of described stainless steel mesh is for being less than or equal to 2mm;
Described mycelia chamber comprises the container of a vertical placement and top opening, offers aperture at the wall of a container face, and the outside wall surface of described container is bonded with one deck nylon screen, also is provided with one deck stainless steel mesh on the nylon screen of container outer wall face; Wherein, the aperture of aperture is 2~4cm on the described container, and the centre distance of adjacent two apertures is 4~7cm; The aperture of described nylon screen is 20~45 μ m; The aperture of described stainless steel mesh is for being less than or equal to 2mm;
Described soil chamber comprises the container of a vertical placement and top opening, offers aperture at the wall of a container face, and the outside wall surface of described container is bonded with one deck nylon screen, also is provided with one deck stainless steel mesh on the nylon screen of container outer wall face; Wherein, the aperture of aperture is 2~4cm on the described container, and the centre distance of adjacent two apertures is 4~7cm; The aperture of described nylon screen is for being less than or equal to 5 μ m; The aperture of described stainless steel mesh is for being less than or equal to 2mm;
Volume of a container and the shape of described formation root system chamber, mycelia chamber and soil chamber are identical.
In the technique scheme:
The container of described formation root system chamber, mycelia chamber and soil chamber is cylindricality or square, is preferably cylindrical.The height of container can determine that preferred heights is 20~40cm according to mycorhiza, the degree of depth of mycelia under the distribution face of land; As for the bore of container should not be too greatly also unsuitable too small (the container bore is excessive, can produce big disturbance to the surrounding soil environment, put it into underground after, mycelia on every side needs the time more of a specified duration just can grow in the device; Container is too small, and when measuring respiratory rate, the outside soil gas of device may interfere with measures degree of accuracy), the scope of choosing of bore is 15~25cm usually.Described container can be to adopt Polyvinylchloride (PVC) material, metal material, foam or other material to make container.
The aperture of aperture is preferably 3cm on the container of described formation root system chamber, mycelia chamber and soil chamber.
The aperture of the stainless steel mesh of described formation root system chamber, mycelia chamber and soil chamber is 1.5~2mm.
The aperture of the nylon screen of described formation mycelia chamber is preferably 30~40 μ m.
The aperture of the nylon screen of described formation soil chamber is preferably 1~3 μ m.
Compared with prior art, other microorganism passes in root system described in the utility model chamber permission plant radicula, mycelia and the soil, and the respiration of mensuration has comprised that other composition is breathed in root system respiration, mycelia breathing and the soil; Other microorganism passes in mycelia chamber permission mycelia and the soil, and the respiration of mensuration is other microbial respiratory in mycelia breathing and the soil; The soil chamber has stoped entering of plant radicula and mycelia, and other microorganism in the soil is only arranged, and the respiration that records is other microbial respiratory in the soil; According to the above-mentioned breathing that records, can draw root system respiration and mycelia breathing component proportion in the soil respiration by concrete formula calculating.
Description of drawings
Fig. 1 is the structural representation of root system chamber in the device described in the utility model;
Fig. 2 is the structural representation of mycelia chamber in the device described in the utility model;
Fig. 3 is the structural representation of soil chamber in the device described in the utility model;
Fig. 4 is the structural representation that constitutes the container of root system chamber, mycelia chamber and soil chamber in the device described in the utility model;
Fig. 5 is the structural representation that constitutes the nylon screen of mycelia chamber in the device described in the utility model;
Fig. 6 is the structural representation that constitutes the stainless steel mesh of root system chamber, mycelia chamber and soil chamber in the device described in the utility model.
Number in the figure is:
Root system chamber 1; 2 containers; The 2-1 aperture; 3 stainless steel mesh; 4 nylon screens; Mycelia chambers 5; Soil chambers 6.
Embodiment
Fig. 1~3 are the structural representation of a kind of embodiment of device described in the utility model, comprise root system chamber 1, mycelia chamber 5 and soil chamber 6, wherein,
Fig. 1 is the structural representation of root system chamber 1 in this embodiment, it comprises the container 2 of a vertical placement and top opening, wall at container 2 offers aperture 2-1 (as shown in Figure 4), in the outside wall surface of container 2 one deck stainless steel mesh 3 (as shown in Figure 6) is arranged fixedly; The aperture of aperture 2-1 is 2~4cm on the described container 2, and the centre distance between adjacent two aperture 2-1 is 4~7cm; The aperture of described stainless steel mesh 3 is for being less than or equal to 2mm.
Fig. 2 is the structural representation of mycelia chamber 5 in this embodiment, it comprises the container 2 of a vertical placement and top opening, wall at container 2 offers aperture 2-1 (as shown in Figure 4), outside wall surface at container 2 is bonded with one deck nylon screen 4 (as shown in Figure 5), and one deck stainless steel mesh 3 (as shown in Figure 6) is also fixedly arranged on the nylon screen 4 of container 2 outside wall surface; The aperture of aperture 2-1 is 2~4cm on the described container 2, and the centre distance between adjacent two aperture 2-1 is 4~7cm; The aperture of described nylon screen 4 is 20~45 μ m; The aperture of described stainless steel mesh 3 is for being less than or equal to 2mm.
Fig. 3 is the structural representation of soil chamber 6 in this embodiment, it comprises the container 2 of a vertical placement and top opening, wall at container 2 offers aperture 2-1 (as shown in Figure 4), (its structure is identical with the structure of mycelia shown in Figure 2 chamber 5 nylon screens 4 to be bonded with one deck nylon screen 4 in the outside wall surface of container 2, just its aperture is different), on the nylon screen 4 of container 2 outside wall surface, also be provided with one deck stainless steel mesh 3 (as shown in Figure 6); The aperture of aperture 2-1 is 2~4cm on the described container 2, and the centre distance between adjacent two aperture 2-1 is 4~7cm; The aperture of described nylon screen 4 is for being less than or equal to 5 μ m; The aperture of described stainless steel mesh 3 is for being less than or equal to 2mm.
In the above-described embodiment, constitute root system chamber 1, mycelia chamber 5 and soil chamber 6 container 2 be the cylinder bodily form, their concrete same height and bores, wherein high is 35cm, diameter is 20cm, adopt pvc pipe and PVC plate to make, concrete manufacturing process can be: at first selecting diameter for use is the pvc pipe that the pvc pipe material of 20cm is cut into the 35cm height; Get the PVC plate again and cut out the plectane that a diameter is 20cm, with polyvinyl chloride adhesive plectane is bonded to an end of cutting out good pvc pipe, as the base plate of container 2; This pvc pipe and plectane constitute the wall of container 2 together, so far then obtain the cylindrical container 2 in the present embodiment.In this embodiment, the aperture of container 2 aperture 2-1 that should guarantee to constitute root system chamber 1, mycelia chamber 5 and soil chamber 6 is consistent with the centre distance of adjacent two aperture 2-1.
In the above-mentioned embodiment; the shape of container 2 and material all can be selected and be replaced as required; also available commercially available other has only the container 2 of a upper shed to substitute; but all belonging to those skilled in the art, these replace or common practise being equal on the utility model basis, should be in protection domain of the present utility model.
The method that adopts said apparatus to measure the breathing of other microorganism in root system, mycelia and the soil is:
1) in the test site, choose a big tree, should set the hole of digging out 9 diameter 20cm, dark 35cm in 3~5m scope greatly in distance, and 3 root system chambers 1,3 mycelia chambers 5 and 3 soil chambers 6 be put into the hole respectively, and the soil that digs out is inserted each root system chamber 1, mycelia chamber 5 and soil chamber 6 respectively;
2) after above-mentioned each chamber is imbedded underground 4~6 months, utilize soil respiration measuring system (Li-6400-09CO 2Analysis cell (LI-COR company, United States Nebraska Lincoln city)), measure the respiratory rate of root system chamber 1, mycelia chamber 5 and soil chamber 6 respectively.According to 3 replication results, average, calculate the ratio of other microbial respiratory of root system of plant, mycelia and soil in breathe root system chamber 1.Specifically calculate by following formula:
Figure BDA00003024426400041
Wherein, root system chamber RRoot system chamber 1 respiratory rate that expression records, the mycelia chamber RMycelia chamber 5 respiratory rates that expression records, the soil chamber RSoil chamber 6 respiratory rates that expression records, r RootRepresent the ratio of pure root system respiration in breathe root system chamber 1, r BacteriumThe expression mycelia breathes the ratio in breathe root system chamber 1, r SoilThe ratio of other microbial respiratory in breathe root system chamber 1 in the expression soil.

Claims (7)

1. be used for measuring the device of root system of plant and mycelia breathing, comprise buried root system chamber (1), mycelia chamber (5) and soil chamber (6), it is characterized in that:
Described root system chamber (1) comprises vertical a placement and the container (2) of top opening, offers aperture (2-1) at the wall of container (2), and the outside wall surface of described container (2) is provided with one deck stainless steel mesh (3); Wherein, the aperture that described container (2) is gone up aperture (2-1) is 2~4cm, and the centre distance of adjacent two apertures (2-1) is 4~7cm; The aperture of described stainless steel mesh (3) is for being less than or equal to 2mm;
Described mycelia chamber (5) comprises the container (2) of a vertical placement and top opening, wall at container (2) offers aperture (2-1), the outside wall surface of described container (2) is bonded with one deck nylon screen (4), also is provided with one deck stainless steel mesh (3) on the nylon screen (4) of container (2) outside wall surface; Wherein, the aperture that described container (2) is gone up aperture (2-1) is 2~4cm, and the centre distance of adjacent two apertures (2-1) is 4~7cm; The aperture of described nylon screen (4) is 20~45 μ m; The aperture of described stainless steel mesh (3) is for being less than or equal to 2mm;
Described soil chamber (6) comprises the container (2) of a vertical placement and top opening, wall at container (2) offers aperture (2-1), the outside wall surface of described container (2) is bonded with one deck nylon screen (4), also is provided with one deck stainless steel mesh (3) on the nylon screen (4) of container (2) outside wall surface; Wherein, the aperture that described container (2) is gone up aperture (2-1) is 2~4cm, and the centre distance of adjacent two apertures (2-1) is 4~7cm; The aperture of described nylon screen (4) is for being less than or equal to 5 μ m; The aperture of described stainless steel mesh (3) is for being less than or equal to 2mm;
Volume and the shape of the container (2) of described formation root system chamber (1), mycelia chamber (5) and soil chamber (6) are identical.
2. according to claim 1 for measuring the device that root system of plant and mycelia breathe, it is characterized in that: described container (2) is cylindricality or square.
3. according to claim 2 it is characterized in that: the height of described container (2) is 20~40cm for measuring the device that root system of plant and mycelia breathe, and bore is 15~25cm.
4. according to each described device for mensuration root system of plant and mycelia breathing in the claim 1~3, it is characterized in that: the aperture that described container (2) is gone up aperture (2-1) is 3cm.
5. according to each described device for mensuration root system of plant and mycelia breathing in the claim 1~3, it is characterized in that: the aperture of the stainless steel mesh (3) of described formation root system chamber (1), mycelia chamber (5) and soil chamber (6) is 1.5~2mm.
6. according to each described device for mensuration root system of plant and mycelia breathing in the claim 1~3, it is characterized in that: the aperture of the nylon screen (4) of described formation mycelia chamber (5) is 30~40 μ m.
7. according to each described device for mensuration root system of plant and mycelia breathing in the claim 1~3, it is characterized in that: the aperture of the nylon screen (4) of described formation soil chamber (6) is 1~3 μ m.
CN2013201737444U 2013-04-09 2013-04-09 Device for determining respiration of plant root system and mycelia Expired - Fee Related CN203191369U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106982628A (en) * 2017-04-01 2017-07-28 内蒙古农业大学 A kind of interior growth core system and method for determining desert steppe root biomass
CN108845105A (en) * 2018-06-08 2018-11-20 河南大学 A kind of soil respiration ring for soil carbon flux monitoring
CN110702857A (en) * 2019-10-18 2020-01-17 浙江农林大学 Method for measuring phyllostachys pubescens rhizome respiration flux

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106982628A (en) * 2017-04-01 2017-07-28 内蒙古农业大学 A kind of interior growth core system and method for determining desert steppe root biomass
CN108845105A (en) * 2018-06-08 2018-11-20 河南大学 A kind of soil respiration ring for soil carbon flux monitoring
CN110702857A (en) * 2019-10-18 2020-01-17 浙江农林大学 Method for measuring phyllostachys pubescens rhizome respiration flux

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