CN104789519A - Culture medium for in vitro fast growth of dendrobium officinale - Google Patents
Culture medium for in vitro fast growth of dendrobium officinale Download PDFInfo
- Publication number
- CN104789519A CN104789519A CN201510213183.XA CN201510213183A CN104789519A CN 104789519 A CN104789519 A CN 104789519A CN 201510213183 A CN201510213183 A CN 201510213183A CN 104789519 A CN104789519 A CN 104789519A
- Authority
- CN
- China
- Prior art keywords
- substratum
- culture medium
- rhamnolipid
- dendrobium officinale
- lactosi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a culture medium for in vitro fast growth of dendrobium officinale. The culture medium is an MS solid culture medium and further comprises 3-10g/L of rhamnolipid and 2-5g/L of a hydrophilic macromolecular material. By adding the hydrophilic macromolecular material which has a sustained release effect into the culture medium, nutritional substances in the culture medium are promoted to be uniformly distributed, the releasing speed of the nutritional substances is regulated, and lasting and balanced adsorption for the nutritional substances by seedlings is met, so that the growth and development of dendrobium officinale is further promoted. The rhamnolipid which is added into the culture medium has the effects of inhibiting fungi and bacteria, and the pollution on the culture medium can be effectively reduced.
Description
Technical field
The present invention relates to a kind of dendrobium officinale culture medium, particularly the substratum that grows fast of a kind of Herba Dendrobii in vitro.
Background technology
Herba Dendrobii is one of the most rare Chinese medicinal materials of China, and have the laudatory title of " in medicine gold ", it is rich in the multiple bioactive ingredients such as dendrobine, dendrobium polysaccharide, dendramine, have tonifying-Yin and nourishing-stomach, anticancer, protect the health-care effecies such as liver.
Dendrobium officinale requires harsh to natural ecological condition; natural propagation rate is extremely low; poor growth; resource scarcity; owing to excavating for a long time, the deterioration of the ecological environment, many rare kinds are endangered; what be classified as focused protection by country treasures Endangered Medicinal Herb, and this has had a strong impact on the development and utilization of Herba Dendrobii.
General Herba Dendrobii adopts regular MS media on producing, seedlings of Dendrobium officinale is inoculated into after in substratum, because the starting point concentration of nutritive substance various in substratum is higher, the speed that seedling absorbs nutritive substance is slower, Initial stage of culture can produce yellowing leaf, the phenomenon of seedling atrophy, even causes seedling dead.
Summary of the invention
The present invention is directed to above shortcoming, large-scale technical renovation is carried out to it, a kind of substratum is provided to add hydrophilic high molecular material, the rhamnolipid of suitable concentration, can promote that seedling absorbs the equilibrium of nutritive substance, effective minimizing substratum pollutes, in the cycle of further shortening Herba Dendrobii isolated growth, reach the object of nurturing staff in the short period of time.
For achieving the above object, the present invention takes following technical proposals to realize:
The substratum that Herba Dendrobii grows in vitro fast, described substratum is MS solid medium, also comprises 3-10g/L rhamnolipid, and 2-6g/L hydrophilic high molecular material.
Further, described hydrophilic high molecular material is hyaluronic acid, the one in vinylformic acid and acrylic acid derivative.
As the preferred embodiment of one, in described substratum, also comprise 6-10g/L honey.
In addition, in described substratum, also semi-lactosi is comprised, one or more in maltose and seminose.
Preferably, 8-12g/L semi-lactosi is also comprised in described substratum.
Further, 40-70g/L sweet potato mash is also comprised in described substratum.
Therefore, as a preferred embodiment of the present invention, described substratum is MS solid medium, also comprises 4-8g/L rhamnolipid, and 3-5g/L polyacrylic acid, 7-9g/L honey, 9-11g/L semi-lactosi, 50g/L sweet potato mash.
More excellent, described substratum is MS solid medium, also comprises 6g/L rhamnolipid, and 4g/L polyacrylic acid, 8g/L honey, 10g/L semi-lactosi, 50g/L sweet potato mash.
In a word, beneficial effect of the present invention is:
Dendrobium officinale culture medium provided by the invention,
Add absorbent polymer material in basic MS culture medium, there is slow releasing function.Absorbent polymer material, as the carrier of nutritive ingredient, can promote being uniformly distributed of substratum Middle nutrition material; Regulate the release rate of nutritive substance, thus realize the relatively stable of substratum Middle nutrition material concentration, meet the lasting equilibrium of seedling to nutritive substance and absorb, promote seedlings of Dendrobium officinale isolated growth further.
Rhamnolipid is a kind of bio-surfactant, can chelating substratum medium trace element, and then improves seedling to the assimilated efficiency of trace element, promotes that seedlings of Dendrobium officinale grows fast further.Rhamnolipid has the effect of Antifungi, bacterium simultaneously, effectively can reduce substratum and pollute.
Honey is as the efficient organic additive of one, and its main component is fructose and glucose, also containing sucrose, maltose and multivitamin, amino acid, yeast, enzyme etc.By adding honey in the medium, supplement various trace elements in substratum, VITAMIN and amino acid, too increase the multiple enzymes such as proteolytic enzyme simultaneously, improves the nutritive ingredient of substratum further, promotes growing of seedlings of Dendrobium officinale.
Further, Ipomoea batatas rich in proteins, starch, pectin, Mierocrystalline cellulose, amino acid, VITAMIN and several mineral materials, the nutrition-allocated proportion in adjustable substratum, experiment proves to be conducive to cultivating healthy and strong seedlings of Dendrobium officinale.
Further, add semi-lactosi in the medium, improve carbon source structure and can promote growing of seedlings of Dendrobium officinale further.
Embodiment
Describe the present invention below in conjunction with specific embodiment.
Embodiment 1
Dendrobium candidum tissue culture medium composition is as follows: MS solid medium
Embodiment 2
Dendrobium candidum tissue culture medium composition is as follows:
This MS medium base also added: 6g/L rhamnolipid, and 4g/L polyacrylic acid, 8g/L honey, 10g/L semi-lactosi, 50g/L sweet potato mash.
Embodiment 3
Dendrobium candidum tissue culture medium composition is as follows:
This MS medium base also added: 3g/L rhamnolipid, and 2g/L polyacrylic acid, 6g/L honey, 8g/L semi-lactosi, 40g/L sweet potato mash.
Embodiment 4
Dendrobium candidum tissue culture medium composition is as follows:
This MS medium base also added: 4g/L rhamnolipid, and 3g/L polyacrylic acid, 7g/L honey, 9g/L semi-lactosi, 50g/L sweet potato mash.
Embodiment 5
Dendrobium candidum tissue culture medium composition is as follows:
This MS medium base also added: 8g/L rhamnolipid, and 5g/L polyacrylic acid, 9g/L honey, 11g/L semi-lactosi, 50g/L sweet potato mash.
Embodiment 6
Dendrobium candidum tissue culture medium composition is as follows:
This MS medium base also added: 10g/L rhamnolipid, and 6g/L polyacrylic acid, 10g/L honey, 12g/L semi-lactosi, 70g/L sweet potato mash.
Comparative example 1
Six groups of experiments are set, the substratum in embodiment 1-6 is adopted to carry out isolated culture to Herba Dendrobii respectively, often group is using 650ML orchid bottle as culture vessel, loading volume in each culture vessel is the substratum of 150mL, then high-temperature sterilization is carried out, Herba Dendrobii seedling (Seedling Height is 2.5-3.0cm) is aseptically accessed after cooling, inoculum size is every bottle of 30 strains, then group training indoor cultivation is put into, culture temperature is 25 DEG C, intensity of illumination is 2000lux, cultivates after 90 days, and the growth indexes of each group seedlings of Dendrobium officinale is as shown in the table:
As can be seen from the above table, relative to basic MS substratum, culture medium prescription of the present invention all has obvious promoter action to the growth of Herba Dendrobii, and effectively reduces pollution rate.Wherein the culture medium prescription of embodiment 2 is optimum to the growth-promoting effect of Herba Dendrobii.
Although the present invention with preferred embodiment openly as above; but it is not for limiting the present invention; any those skilled in the art without departing from the spirit and scope of the present invention; the Method and Technology content of above-mentioned announcement can be utilized to make possible variation and amendment to technical solution of the present invention; therefore; every content not departing from technical solution of the present invention; according to technical spirit of the present invention to any simple modification made for any of the above embodiments, equivalent variations and modification, all belong to the protection domain of technical solution of the present invention.
Claims (8)
1. the substratum that grows fast in vitro of Herba Dendrobii, it is characterized in that, described substratum is MS solid medium, also comprises 3-10g/L rhamnolipid, and 2-6g/L hydrophilic high molecular material.
2. substratum according to claim 1, is characterized in that, described hydrophilic high molecular material is hyaluronic acid, the one in vinylformic acid and acrylic acid derivative.
3. substratum according to claim 1, is characterized in that, also comprises 6-10g/L honey in described substratum.
4. substratum according to claim 1, is characterized in that, also comprises semi-lactosi in described substratum, one or more in maltose and seminose.
5. substratum according to claim 4, is characterized in that, also comprises 8-12g/L semi-lactosi in described substratum.
6. substratum according to claim 1, is characterized in that, also comprises 40-70g/L sweet potato mash in described substratum.
7. the substratum according to claim 1-6 any one, is characterized in that, described substratum is MS solid medium, also comprises 4-8g/L rhamnolipid, and 3-5g/L polyacrylic acid, 7-9g/L honey, 9-11g/L semi-lactosi, 50g/L sweet potato mash.
8. substratum according to claim 7, is characterized in that, described substratum is MS solid medium, also comprises 6g/L rhamnolipid, and 4g/L polyacrylic acid, 8g/L honey, 10g/L semi-lactosi, 50g/L sweet potato mash.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510213183.XA CN104789519B (en) | 2015-04-29 | 2015-04-29 | A kind of culture medium of the in vitro fast-growth of dendrobium candidum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510213183.XA CN104789519B (en) | 2015-04-29 | 2015-04-29 | A kind of culture medium of the in vitro fast-growth of dendrobium candidum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104789519A true CN104789519A (en) | 2015-07-22 |
| CN104789519B CN104789519B (en) | 2017-12-12 |
Family
ID=53554714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510213183.XA Active CN104789519B (en) | 2015-04-29 | 2015-04-29 | A kind of culture medium of the in vitro fast-growth of dendrobium candidum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104789519B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106916018A (en) * | 2017-04-07 | 2017-07-04 | 滨州市和德农业科技有限公司 | A kind of Volvaria volvacea cultivation base-material and preparation method thereof |
| CN107625663A (en) * | 2017-09-13 | 2018-01-26 | 西安瑞捷生物科技有限公司 | A kind of basic aqua of glycolipid containing high-purity rhamnazin and its application |
| CN110800618A (en) * | 2019-12-19 | 2020-02-18 | 山西景致苗木有限公司 | Plant rooting and seedling strengthening culture medium |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7405181B2 (en) * | 2005-01-13 | 2008-07-29 | National Taiwan University | Bio-fertilizer composition for promoting growth or orchid plants and application |
| CN101548644A (en) * | 2009-03-25 | 2009-10-07 | 刘星华 | Tissue culturing method for applying nanotechnology to promote fast proliferation of dendrobium officinale |
| CN101845468A (en) * | 2010-03-30 | 2010-09-29 | 湖州紫金生物科技有限公司 | Preparation method and application of rhamnolipid |
| CN102948368A (en) * | 2012-11-13 | 2013-03-06 | 安徽新津铁皮石斛开发有限公司 | Tissue culture method of dendrobium candidum |
| CN102986464A (en) * | 2012-12-11 | 2013-03-27 | 浙江大学 | Method for preventing and treating watermelon wilt and promoting growth of watermelon seedlings |
| CN103749301A (en) * | 2014-01-14 | 2014-04-30 | 桂林丰润莱生物科技有限公司 | Culture medium capable of reducing pollution rate of dendrobium officinale tissue culture seedlings |
-
2015
- 2015-04-29 CN CN201510213183.XA patent/CN104789519B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7405181B2 (en) * | 2005-01-13 | 2008-07-29 | National Taiwan University | Bio-fertilizer composition for promoting growth or orchid plants and application |
| CN101548644A (en) * | 2009-03-25 | 2009-10-07 | 刘星华 | Tissue culturing method for applying nanotechnology to promote fast proliferation of dendrobium officinale |
| CN101845468A (en) * | 2010-03-30 | 2010-09-29 | 湖州紫金生物科技有限公司 | Preparation method and application of rhamnolipid |
| CN102948368A (en) * | 2012-11-13 | 2013-03-06 | 安徽新津铁皮石斛开发有限公司 | Tissue culture method of dendrobium candidum |
| CN102986464A (en) * | 2012-12-11 | 2013-03-27 | 浙江大学 | Method for preventing and treating watermelon wilt and promoting growth of watermelon seedlings |
| CN103749301A (en) * | 2014-01-14 | 2014-04-30 | 桂林丰润莱生物科技有限公司 | Culture medium capable of reducing pollution rate of dendrobium officinale tissue culture seedlings |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106916018A (en) * | 2017-04-07 | 2017-07-04 | 滨州市和德农业科技有限公司 | A kind of Volvaria volvacea cultivation base-material and preparation method thereof |
| CN107625663A (en) * | 2017-09-13 | 2018-01-26 | 西安瑞捷生物科技有限公司 | A kind of basic aqua of glycolipid containing high-purity rhamnazin and its application |
| CN110800618A (en) * | 2019-12-19 | 2020-02-18 | 山西景致苗木有限公司 | Plant rooting and seedling strengthening culture medium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104789519B (en) | 2017-12-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102351605B (en) | Culture method for liquid fermentation of rare edible-medicinal fungus Sparassis crispa and special medium thereof | |
| CN104030846A (en) | Mushroom culture medium and preparing method thereof | |
| CN101983955A (en) | Phellinus igniarius mycelium culture medium and process for fermenting phellinus igniarius using same | |
| CN104911169B (en) | A kind of method for training cordyceps mycelium and fibrinolysin processed | |
| CN104789519A (en) | Culture medium for in vitro fast growth of dendrobium officinale | |
| CN103922851A (en) | Black fungus liquid medium and black fungus cultural method | |
| CN104193508A (en) | Method for preparing composite organic type environment-friendly seedling culture medium block | |
| CN101514116A (en) | Formula for culture medium for culturing mushrooms with bagasse | |
| CN103664320A (en) | Additive for soilless-culture sterile nutrient liquor | |
| CN101233863A (en) | High active biological nutrition root-inducing powder | |
| CN105016906B (en) | A kind of edible fungus culturing matrix and preparation method thereof based on olive pomace | |
| CN102523922A (en) | Method for cultivating Poria cocos fruiting bodies | |
| CN102701857B (en) | Ganoderma lucidum culture medium prepared from coix seed shells and coix seed straws | |
| CN107242252A (en) | A kind of bioactivity nutrition root-inducing powder | |
| CN106588199A (en) | Agriculture organic fertilizer | |
| CN108624512A (en) | Solid fermentation matrix, preparation method and the method for cultivating mycorhiza biological agent | |
| CN106747886A (en) | A kind of biological organic fertilizer | |
| CN105724207A (en) | Matrix for enhancing use effect of arbuscular mycorrhizal fungi and preparation method and application of matrix | |
| CN105230490A (en) | In vitro preservation culture medium for cassava embryogenic callus | |
| CN105255736A (en) | Preservation method of hypsizigus marmoreus strain | |
| CN107417338A (en) | A kind of culture medium of edible fungus and preparation method thereof | |
| CN1392252A (en) | Cultivating method for increasing sweet wormwood organ biomass and artemisine content | |
| CN104030850B (en) | A kind of mushroom culture medium adding hop residue and preparation method thereof | |
| CN103348872B (en) | A kind of method utilizing changing food waste to cultivate Cordyceps militaris | |
| CN103864477A (en) | Liquid strain medium of pure natural domestic fungus, manufacturing method and method for culturing strain of pure natural domestic fungus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |