CN104784240B - A kind of mouthwash containing plants essential oil and preparation method thereof - Google Patents
A kind of mouthwash containing plants essential oil and preparation method thereof Download PDFInfo
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- CN104784240B CN104784240B CN201510208009.6A CN201510208009A CN104784240B CN 104784240 B CN104784240 B CN 104784240B CN 201510208009 A CN201510208009 A CN 201510208009A CN 104784240 B CN104784240 B CN 104784240B
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Abstract
The invention discloses a kind of mouthwash containing plants essential oil and preparation method thereof, aim to provide a kind of with sterilization, anti-inflammatory, analgesia, hemostatic function, and being capable of pre- preventing parodontitis, gingivitis, bleeding gums, halitosis and canker sore, it can take, the mouthwash containing plants essential oil having no toxic side effect；Technical points：The mouthwash includes horseradish essential oil and olive oil mixture, second alcohol and water.
Description
Technical field
The present invention relates to a kind of mouthwash and preparation method thereof, is a kind of mouthwash containing plants essential oil specifically
And preparation method thereof.
Background technology
In recent years, as the living standard of economic development and people improve, oral hygiene and oral health increasingly by
To the attention of people.At present, oral health issue particularly periodontitis, gingivitis, bleeding gums, halitosis and canker sore etc. into
For the puzzlement of general population.The method of preventive maintenance oral health is various, but safeguards the health in oral cavity, effect in a conventional manner
It is often bad, the high incidence of 90% gum disease is shown with evidence, and be continuously increased the hair of oral cavity related system disease
Sick rate.
Gargled with oil, because the adhesion of oil is high, be easier to be full of Buccodental, gum and mucous membrane than water, and bacterium has parent
Oiliness, oil is easier to allow bacterium to be melted into wherein than water, and passes through sublingual absorption, rapidly takes the nutrition of oil in blood to, and will
Bacterium and toxin in blood are discharged in oil.Oil, which is gargled, provides a good method, improves the health of oral cavity and body system.
Horseradish (Wasabi japonica Matsum) also known as mountain dish, scurvy grass, are that Cruciferae Eutrema is perennial
Half cloudy raw herbaceous plant.Horseradish is a kind of medicinal and edible plant, has very high economic value, this is because containing in horseradish
The various trace elements that substantial amounts of amino acid and human body need, and further comprises abundant isosulfocyanate compound.Mountain
Certain herbaceous plants with big flowers has strong acid and pungent fragrance, some researches show that, horseradish also have prevention decayed tooth, except halitosis, sterilize, help and disappear
The effect such as change.But the isosulfocyanate compound due to containing in horseradish has very strong volatility, rarely utilizes at present
Horseradish extract develops relevant horseradish product, currently yet there are no to use and gargles by the use of horseradish extract horseradish essential oil as oil
The report of aquatic products.
The content of the invention
In view of the above-mentioned problems, the object of the present invention is to provide one kind to have sterilization, anti-inflammatory, analgesia, hemostatic function, and energy
Enough effectively pre- preventing parodontitis, gingivitis, bleeding gums, halitosis and canker sore, have no toxic side effect being gargled containing plants essential oil of can taking
Saliva, the present invention also provides the preparation method of the mouthwash.
To achieve these goals, first technical solution provided by the invention is such：
This contains the mouthwash of plants essential oil, including horseradish essential oil and olive oil mixture, second alcohol and water.
Further, the above-mentioned mouthwash containing plants essential oil, the mouthwash horseradish containing 3wt%-12wt% essence
Oil and olive oil mixture, 15-25wt% ethanol, surplus is water.
Further, the mass ratio of the above-mentioned mouthwash containing plants essential oil, the horseradish essential oil and olive oil
For：1：19~29.
Further, the above-mentioned mouthwash containing plants essential oil, the mouthwash also contain 6-15wt% sorbs
Alcohol.
To prepare the above-mentioned mouthwash containing plants essential oil, present invention also offers the preparation method of the mouthwash：
The first preparation method of mouthwash provided by the invention containing plants essential oil is：By horseradish essential oil and olive oil
After mixing, be added in ethanol and stir evenly, be eventually adding distilled water and be stirred into transparency liquid, to obtain the final product.
Second of preparation method of the mouthwash provided by the invention containing plants essential oil be：By horseradish essential oil and olive oil
After mixing, it is added in ethanol, adds sorbierite and be mixed evenly, is eventually adding distilled water and is stirred into transparent liquid
Body, to obtain the final product.
Final object of the present invention be to provide the above-mentioned mouthwash for containing plants essential oil as pre- preventing parodontitis or
The purposes of person's gingivitis or bleeding gums, halitosis or canker sore ancillary drug.
Compared with prior art, technical solution provided by the invention extracts horseradish essential oil from natural plants horseradish, and
Mixed in proportion with olive oil, then the mouthwash that ethanol, sorbierite are mixed to get, the mouthwash preparation method is simple, has no and adds
Add any preservative, having no toxic side effect to take orally；And with good sterilization, anti-inflammatory, analgesia, hemostatic function, Neng Gouyou
Imitate pre- preventing parodontitis, gingivitis, bleeding gums, halitosis and canker sore.
Brief description of the drawings
Fig. 1 is inhibition figure of the mouthwash containing plants essential oil to several food-borne strains
First row is the sample mouthwash of embodiment 7 wherein in A, B, C culture dish, and the 3rd row is to sell dioxygen water germicide sun
Property control, second row for methylene chloride control, the 4th row is DMSO negative controls；First row is embodiment 7 in D culture dishes
Sample mouthwash, the commercially available dioxygen water germicide positive control of second row, the 3rd row is methylene chloride control, and the 4th row is
DMSO negative controls.Food-borne examination strain is wherein included in figure：A staphylococcus epidermis, B staphylococcus aureuses, C are husky
Door Salmonella and D Sarcina luteas.
Embodiment
The present invention is further illustrated below by the mode of embodiment, but does not form any limitation of the invention, is appointed
The modification for the limited number of time that who is made in scope of the presently claimed invention is still in scope of the presently claimed invention.
Embodiment 1
A kind of mouthwash containing plants essential oil provided by the invention, by 30g horseradish essential oil and olive oil mixture, 250g
Ethanol, 720g water composition.
Wherein：Horseradish essential oil 1.5g, the horseradish essential oil are the essential oils as obtained from extracting natural plants horseradish；Olive
Olive oil 28.5g.
Embodiment 2
Mouthwash of the another kind containing plants essential oil provided by the invention, by 120g horseradish essential oil and olive oil mixture,
150g ethanol, 6-15wt%
730g water forms.
Wherein：Horseradish essential oil 4g, the horseradish essential oil are the essential oils as obtained from extracting natural plants horseradish；Olive
Oily 116g.
Embodiment 3
Mouthwash of the another kind containing plants essential oil provided by the invention, by 100g horseradish essential oil and olive oil mixture,
200g ethanol, 700g water composition.
Wherein：Horseradish essential oil 4g, the horseradish essential oil are the essential oils as obtained from extracting natural plants horseradish；Olive
Oily 96g.
The preparation method for the mouthwash that any one in embodiment 1 to 3 contains plants essential oil is：Appoint by embodiment 1 to 3
A kind of mass number of anticipating weighs each component, after the horseradish essential oil weighed is mixed with olive oil, is added in ethanol and stirs evenly,
Be eventually adding distilled water and be stirred into transparency liquid, to obtain the final product.
Embodiment 4
Provided by the invention one another mouthwash of the kind containing plants essential oil, by 30g horseradish essential oil and olive oil mixture,
250g ethanol, 60g sorbierites, 660g water composition.
Wherein：Horseradish essential oil 1.5g, the horseradish essential oil are the essential oils as obtained from extracting natural plants horseradish；Olive
Olive oil 28.5g.
Embodiment 5
Mouthwash of the another kind containing plants essential oil provided by the invention, by 120g horseradish essential oil and olive oil mixture,
150g ethanol, 150g sorbierites, 580g water composition.
Wherein：Horseradish essential oil 4g, the horseradish essential oil are the essential oils as obtained from extracting natural plants horseradish；Olive
Oily 116g.
Embodiment 6
Mouthwash of the another kind containing plants essential oil provided by the invention, by 100g horseradish essential oil and olive oil mixture,
200g ethanol, 100g sorbierites, 600g water composition.
Embodiment 7
Mouthwash of the another kind containing plants essential oil provided by the invention, by 50g horseradish essential oil and olive oil mixture,
200g ethanol, 100g sorbierites, 650g water composition.
Wherein：Horseradish essential oil 2g, the horseradish essential oil are the essential oils as obtained from extracting natural plants horseradish；Olive
Oily 48g.
Embodiment 8
Mouthwash of the another kind containing plants essential oil provided by the invention, by 100g horseradish essential oil and olive oil mixture,
180g ethanol, 80g sorbierites, 640g water composition.
Wherein：Horseradish essential oil 4g, the horseradish essential oil are the essential oils as obtained from extracting natural plants horseradish；Olive
Oily 96g.
The preparation method for the mouthwash that any one in embodiment 4 to 8 contains plants essential oil is：Appoint by embodiment 4 to 8
A kind of mass number of anticipating weighs each component, after horseradish essential oil is mixed with olive oil, is added in ethanol, adds sorbierite and mixes
Conjunction stirs evenly, and is eventually adding distilled water and is stirred into transparency liquid, to obtain the final product.
The advantages of in order to better illustrate technical solution provided by the invention, be given below provided by the invention containing plant
The mouthwash Clinical practice case of essential oil
Case 1
Mrs Liu, 27 years old, decayed tooth position periodontal swelling in oral cavity, inflammation were serious, pain, and bradymassesis, takes cephalo drawing
Surely the antibacterials such as antibiotic and tinidazole tablets are waited not improve.Using mouthwash provided by the invention, each oral cavity, which contains, gargles 5 points
Clock, it is 3-4 times daily, next day feeling of pain reduce, can normal laboratory rodent chow, using two days later, inflammation gradually disappears with swelling, can
Normally to dehisce to be engaged, positive effect.
Case 2
Mr. Zhang, 22 years old, because in oral cavity wisdom tooth grow, cause second molar be subject to squeeze force, pain is serious, second molar week
Enclose gum swelling, it is impossible to chew, taking the antibiotic a few days such as metronidazole does not improve.Using mouthwash of the present invention, each oral cavity
Containing gargling 5 minutes, each 1 time of before or after meals, 1 time when pain is serious, next day pain relief, swelling is disappeared, and symptom substantially changes after two days
It is kind.
Case 3
Mr. Zhao, 56 years old, has longer smoking history, has peculiar smell in oral cavity, suffers from canker sore, and nearly 1 year of recurrent exerbation is hard
Hold and use mouthwash of the present invention two months, 4-5 times daily, every time containing gargling 5 minutes, oral peculiar smell eliminates, and canker sore situation
Take a favorable turn, temporarily without recurrence present situation.
There is higher antibacterial, bactericidal activity in order to illustrate plants essential oil mouthwash provided by the invention, this is given below
Invention section active effect experimental example：
Experimental example 1
Suppression test of the plants essential oil mouthwash to food-borne bacterium
This experiment chooses four kinds of food-borne strains and carries out bacteriostatic activity test, is respectively staphylococcus epidermis, golden yellow Portugal
Grape coccus, salmonella and Sarcina lutea.
1st, solid medium is prepared：4g standard mediums, add 100mL ultra-pure waters, and dissolving is boiled in heating；Go out for the first time
Bacterium：Solid medium, surface plate 6cm (6~7), EP pipes 4mL, 100mL beaker (2), pipette tips, ultra-pure water, glass bar, amount
Cylinder；A pipe actication of culture half an hour (in 37 DEG C of water-baths) is taken, solid medium is dispensed into surface plate, and oese is seeded to table
On the ware of face, when activation culture 12 is small.
2nd, fluid nutrient medium：Peptone 1g, dusty yeast 0.5g, sodium chloride 1g, ultra-pure water 100mL, adjust pH7-7.4；The
Two-stage sterilization：Solid medium, fluid nutrient medium, triangular glass rod, filter paper, pipette tips, EP pipes, culture dish (6~7), examination
Pipe and plug sterilize in the lump.Preparation of 0.5 Maxwell than turbid：The 1mL concentrated sulfuric acids are taken to be added in 99mL ultra-pure waters；1.175 chlorination
Barium is dissolved in 100mL ultra-pure waters；Added with the barium chloride solution of 500 μ L of liquid-transfering gun absorption in the dilute sulfuric acid of the 100mL prepared i.e.
.
3rd, Bactericidal test：The strain activated is picked out, different gradient concentrations is diluted to sterile water, pipettes each gradient
The bacteria suspension of concentration and 0.5 Maxwell each 100 μ L of ratio solution are into 96 orifice plates, on multi-function microplate reader, absorbance and 0.5 Maxwell
Than similar as 1.5 × 108CFU/ML bacteria concentrations.On the basis of secondary, 100 times are being diluted with fluid nutrient medium, obtain 1.5 ×
106CFU/M bacteria suspensions.
4th, the solid medium of sterilizing is imported in aseptic flat board (12cm), is pipetted after condensation with the liquid-transfering gun of 1000 μ L
400 μ L concentration are the bacteria suspension of 1.5 × 106CFU/M, and agar surface is slightly compressed to the return home filter paper of a diameter of 6cm of aseptic nipper,
Extract is pipetted with 10 μ L liquid-transfering guns to drop on the scraps of paper, using DMSO as negative control, using 84 thimerosals as positive control.
5th, each 1mL of sample of gradient in 4 is pipetted, adds the bacteria suspension (containing liquid medium) of 1.5 × 105CFU/M.Will
Test tube with sterilizing silica gel plug tight seal after be placed in 37 DEG C of shaking table, 24 it is small when after observation the results are shown in Figure 1.
Bacteriostatic experiment the results show that in Fig. 1 in A, B, C culture dish first row be embodiment 7 sample mouthwash, the 3rd row
Commercially available dioxygen water germicide positive control, second row compare for methylene chloride, and the 4th row is DMSO negative controls；D is cultivated
First row is the sample mouthwash of embodiment 7 in ware, and the commercially available dioxygen water germicide positive control of second row, the 3rd row is solvent two
Chloromethanes compares, and the 4th row is DMSO negative controls.Food-borne examination strain is wherein included in figure：A staphylococcus epidermis,
B staphylococcus aureuses, C salmonellas and D Sarcina luteas.It can be seen from the figure that contain plants essential oil in embodiment 7
Mouthwash has a good fungistatic effect to various examination bacterium strains, and with positive commercially available dioxygen water germicide positive control phase
Than fungistatic effect is more preferably.
Experimental example 2
Section Example lowest bacteria fogging-resistant concentration determining value provided by the invention is given below, its calculation formula is as follows：
According to formula：
Obtain 1 data of table.
Table 1
In above-mentioned table as can be seen that the mouthwash being prepared using technical solution provided by the invention to tested strain most
The value of low Mlc is all relatively low, positive effect.
Claims (4)
- A kind of 1. mouthwash containing plants essential oil, it is characterised in that mouthwash horseradish containing the 3wt%-12wt% essential oil It is water with olive oil mixture, 15-25wt% ethanol, 6-15wt% sorbierites, surplus.
- 2. the mouthwash according to claim 1 containing plants essential oil, it is characterised in that the horseradish essential oil and olive Oil mass ratio be：1：19~29.
- A kind of 3. method for preparing the mouthwash containing plants essential oil that right is wanted described in 1, it is characterised in that by horseradish essential oil with Olive oil mixing after, be added in ethanol, add sorbierite and be mixed evenly, be eventually adding distilled water be stirred into it is saturating Prescribed liquid, to obtain the final product.
- 4. the mouthwash for containing plants essential oil described in claim 1, which is used as, prepares pre- preventing parodontitis either gingivitis or gum The purposes of bleeding, halitosis or canker sore ancillary drug.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105326742A (en) * | 2015-11-12 | 2016-02-17 | 厉政轩 | Anti-infective and antibacterial mouth wash and preparation method thereof |
| CN109125178A (en) * | 2018-10-15 | 2019-01-04 | 安徽万春日化有限公司 | A kind of whitening toothpaste and preparation method thereof effectively removing creamy white spot |
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2015
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| CA2232884A1 (en) * | 1997-04-02 | 1998-10-02 | Farmo-Nat Ltd. | Synergistic herbal extracts |
| CN1813671A (en) * | 2005-11-17 | 2006-08-09 | 上海交通大学 | Natural aromatic plant distillate type gargle |
| CN103153272A (en) * | 2010-07-19 | 2013-06-12 | 宝洁公司 | Compositions comprising derivatives of essential oil compounds and use in personal care products |
| CN101991521A (en) * | 2010-11-03 | 2011-03-30 | 中国热带农业科学院农产品加工研究所 | Tea tree oil mouth wash and preparation method thereof |
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Effective date of registration: 20220415 Address after: 310018 Room 903, building 1, No. 265, Chengrui street, Xiasha street, Qiantang new area, Hangzhou, Zhejiang Patentee after: Anyu Biotechnology (Hangzhou) Co.,Ltd. Address before: 510006 room 706, No.4 engineering building, Guangdong University of technology, No.100 Waihuan West Road, Guangzhou University City, Panyu District, Guangzhou City, Guangdong Province Patentee before: GUANGDONG University OF TECHNOLOGY |