CN104770231A - Method for strengthening abiotic environmental stress resistance of crops - Google Patents
Method for strengthening abiotic environmental stress resistance of crops Download PDFInfo
- Publication number
- CN104770231A CN104770231A CN201510171234.7A CN201510171234A CN104770231A CN 104770231 A CN104770231 A CN 104770231A CN 201510171234 A CN201510171234 A CN 201510171234A CN 104770231 A CN104770231 A CN 104770231A
- Authority
- CN
- China
- Prior art keywords
- seedling
- rice
- rape
- nutrient solution
- crop
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a method for strengthening the abiotic environmental stress resistance of crops. The method comprises the steps of putting seedlings in a simulation pool of abiotic environmental stress; uniformly sprinkling a plant growth regulator solution containing 1-aminocyclopropane-1-carboxyl and methyl jasmonate at the same time onto the leaves of the seedlings, wherein the sprinkling amount is as the criterion when all the leaves are completely wetted by the growth regulation solution. The method is a growth regulation mode capable of obviously increasing the accumulation of nitric nitrogen at the roots of the crops, so that the abiotic environmental stress resistance of the crops is obviously improved, and the abiotic environmental stress resistance of the crops for heavy metal contamination and salt stress in a growing environment is strengthened.
Description
Technical field
The present invention relates to a kind of crop cultivation and growth control method, be specially a kind of nitrate nitrogen allocation proportion that should be used for increasing crop root by plant growth regulator, thus strengthen the method for crop anti-adversity.
Background technology
The earth about there is the topsoil of 20% be subject to the impact of abiotic stress environments.These soil are not only distributed in desert and area, semidesert, but also are usually distributed in fertile Plain, river valley and these densely populated irrigation region coastal.Abiotic stress environments mainly can be divided into two kinds: wetland is coerced and drought stress.It is the swampy area depending on sea that wetland is coerced, and these areas are subject to the impact of seawater high content of salt and produce salt stress.The desert edge being mainly distributed in inland is coerced in arid area.What some other was coerced affect, and area is mainly inland lake and area, river.Abiotic stress environments refers to that the salinity or heavy metal substance etc. contained in soil or water source has had influence on the normal growth of crop.Therefore, improve the resistance under crop abiotic stress environments condition, set up the complete skill system that a set of raising crop is degeneration-resistant, for alleviating environment stress on the impact of crop with to improve crop yield all most important.
Nitrate nitrogen is the main nitrogenous source of terrestrial plant.In plant corpus, nitrate nitrogen can metabolism be ammonium salt, glutamic acid is changed into by glutamine synthelase/glutamate synthetase (GS-GOGAT) circulation, and glutamic acid can generate other amino acid further, thus participate in building each quasi-molecules such as protein, nucleotide, chlorophyll.And what is more important, the downstream metabolites of nitrate nitrogen itself and many nitrate nitrogens can as the adaptation to environment of the metabolism of signaling molecule regulating plant, growth, growth and plant.Nitrate nitrogen enters after in plant corpus, except small part root assimilation or be stored in vacuole, most of nitrate nitrogen by the long-distance acrial part being transported to plant, can utilize carbon skeleton, energy and reducing power that photosynthesis provides, assimilates as organic nitrogen there.Because direct for carbon and nitrogen metabolism coupling can be got up by the mode of this nitrogen assimilation, there is energy evolutionary edge, therefore become the prioritizing selection of most plants.As can be seen here, the long-distance transport process of nitrate nitrogen determines the allocation proportion of nitrate nitrogen at Plant aboveground and position, underground, and and then determines the efficiency of nitrogen assimilation.
But, when external environment is unfavorable (such as low temperature, the low light level or heavy metal), has more nitrate nitrogen and accumulate in root, be i.e. the nitrate nitrogen reallocation of adverse circumstance induction.This process makes the C N metabolism uncoupling in nitrate nitrogen assimilation process, and energy utilization efficiency may be made to decline.But this physiology course still preserve during evolution and adopt by many plants.A kind of explanation is, in root nitrate nitrogen assimilation can not with the fixing contention reducing power of carbonic acid gas in leaf and energy, be conducive to the growth of plant under adverse environment.Therefore, from this point, nitrate nitrogen reallocation (namely allowing more nitrate-N accumulation under adverse environmental factor in the root system of crop) of adverse circumstance induction has important meaning for the resistance improving plant.
Find in the research of model plant, the nitrate nitrogen reallocation of adverse circumstance induction is mainly by two nitrate nitrogen transport proteins
nRT1.5with
nRT1.8mediation.The former mainly expresses the pericyclic cell at root, and be responsible for the loading of nitrate nitrogen to xylem, the latter mainly expresses the parenchyma cell at xylem, is responsible for the unloading of nitrate nitrogen from xylem.Under normal circumstances, the nitrate nitrogen of root absorption passes through N
rT1.5be contained in and enter xylem vessel, be transferred to acrial part, and acrial part
nRT1.8be offloaded to xylem parenchyma cell, and then by symplastic transport to mesophyll cell.But, when adverse circumstance is come interim, root
nRT1.5expression is lowered, and decreases the loading of nitrate nitrogen to xylem; Meanwhile,
nRT1.8expression raises, at root unloading nitrate nitrogen.By the transcriptional control of this hight coordinate, on the ground, the nitrate nitrogen ratio of under ground portion is able to by fine adjustment under different environmental conditions.And the plant that the nitrate nitrogen reallocation of adverse circumstance induction in body occurs to lack is as more responsive in heavy metal cadmium, salt stress, arid etc. to adverse circumstance.The nitrate nitrogen reallocation that These findings suggest adverse circumstance induction may to reduce energy utilization efficiency for the resistance of cost raising to plant.
Visible, both at home and abroad to the ground of nitrate nitrogen, underground part distributes more research, but how to utilize the long-distance transhipment of nitrate nitrogen still there is no good way to regulate and control crop for the resistance of abiotic stress environments.In addition, the regulation and control of the transcriptional level of this high Collaboration of the long-distance transhipment of nitrate nitrogen all can occur when in the face of multiple adverse circumstance, and the nitrate nitrogen reallocation of hint adverse circumstance induction is a kind of generalised mechanism in the face of adverse circumstance.But how the nitrate nitrogen reallocation of adverse circumstance induction is activated, and is how to play a role and interactional with external environment, still knows little about it after startup.
Summary of the invention
Technical problem to be solved by this invention is, the knowwhy mentioned in above background technology is able to practical application by the interpolation of crop exogeneous growth Auto-regulator (1-amino-cyclopropane-1-carboxylic acid and methyl jasmonate), a kind of growth regulating mode that significantly can increase crop root nitrate-N accumulation is provided, thus significantly improve the resistance of crop to abiotic stress environments, strengthen the resistance of crop for the abiotic stress environments such as heavy metal pollution, salt stress in growing environment.
For solving the problems of the technologies described above, the technical scheme that the present invention proposes is a kind of method strengthening crop environment resistant abiotic stress, comprise the following steps: simulation pond seedling being placed in abiotic stress environments, be spread across on the blade of seedling by the plant growth regulator dissolution homogeneity simultaneously containing 1-amino-cyclopropane-1-carboxylic acid and methyl jasmonate, the amount of sprinkling is as the criterion with the complete moistening all blades of growth regulating agent solution.
Method And Principle: the blade of (1) crop growth regulator (1-amino-cyclopropane-1-carboxylic acid and methyl jasmonate) sprays and the expression of the long-distance transporter gene of nitrate nitrogen can be made to change; (2) the long-distance transporter gene of nitrate nitrogen makes more nitrate nitrogen be assigned to the under ground portion of crop after changing; (3) the accumulation ratio increase of the part of a crop which is beneath the ground nitrate nitrogen significantly can strengthen the resistance of crop.
In above-mentioned method, preferably, described seedling is rice seedling and Brassica Napus Seedling.
In above-mentioned method, preferably, the cultivation of described rice seedling comprises the following steps: water planting adopts Yoshida nutrient solution (International Rice Research Institute Yoshida nutrient solution prescription), a floating expanded polystyrene sheet above culture vessel, thin plate there are 120 holes: horizontally-arranged 12, vertical setting of types 10, 1 pocket is broadcast in every hole, 2, every pocket, it is nylon wire bottom thin plate, seed is broadcast thereon, randomized arrangement, first seed is put into 50 DEG C of baking oven Dormancy breaking 3 days before sowing, vernalization in advance, rice paddy seed after vernalization is all cultivated after 3 ~ 6 days with distilled water, continue to cultivate with Yoshida nutrient solution again.
In above-mentioned method, preferably, the cultivation of described Brassica Napus Seedling comprises the following steps: to be soaked seed by rape seed in volume fraction be 20%, and clorox carries out surface sterilization 30 ~ 50 min, germinate in the earth culture of glass solarium for 3 ~ 6 times with distilled water flushing later, nutrient solution used is He Gelande (Hoagland) complete nutrition liquid, within every 5 days, water one time of nutrition liquid, water 100 ml at every turn, mean temperature 29 DEG C/19 DEG C round the clock in glass solarium, natural humidity and illumination, intensity of illumination is 205 ~ 230 Ue/ (m
2.s).
In above-mentioned method, preferably, in the simulation pond of described abiotic stress environments, salt and Cd stress concentration are respectively 50 ~ 150 mmolL
-1naCl, 50 ~ 200 μm of olL
-1cdCl
2, described paddy rice is transplanted by the density of 6600 plants/acre, and described rape is transplanted by the density of 8000 plants/acre.
In above-mentioned method, preferably, the concentration for the treatment of of described 1-amino-cyclopropane-1-carboxylic acid is 10 ~ 100 μm of olL
-1, the concentration for the treatment of of methyl jasmonate (MeJA) is 50 ~ 200 μm of olL
-1.
In above-mentioned method, preferably, the period of described growth regulator process is from the Seedling Stage after rice transplanting to the pustulation period, and the Seedling Stage after transplanting from rape is to the Rape pod development phase, comprise every 6 days from the Seedling Stage after transplanting and spray a plant growth regulator until the pustulation period entering paddy rice terminates uniformly, and the Rape pod development phase of rape terminates.
In above-mentioned method, preferably, NH is added in the Yoshida nutrient solution of described paddy rice
4nO
3, NaH
2pO
42H
2o, K
2sO
4, CaCl
2, MgSO
47H
2o, makes macronutrient N, P, K, Ca, Mg content in nutrient solution be respectively 35 ~ 45,8 ~ 12,35 ~ 45,35 ~ 4045,35 ~ 45 mg/L; In Yoshida nutrient solution, add MnCl simultaneously
24H
2o, (NH
4)
6mo
7o
242H
2o, H
3bO
3,znSO
47H
2o, CuSO
45H
2o, FeCl
36H
2o, makes micronutrient element Mn, Mo, B, Zn, Cu, Fe content in nutrient solution be respectively 0.4 ~ 0.6,0.04 ~ 0.06,0.18 ~ 0.22,0.018 ~ 0.022,0.008 ~ 0.012,1.8 ~ 2.2 mg/L.
In above-mentioned method, preferably, described rape He Gelande used (Hoagland) complete nutrition liquid composition comprises 4 ~ 6 mmol.L
-1kNO
3, 0.5 ~ 1.5 mmol.L
-1kH
2pO
4, 6 ~ 8 mmol.L
-1mgSO
4, 4 ~ 6 mmol.L
-1ca (NO
3)
2 .4H
2o, 2 ~ 4 mmol.L
-1fe-EDTA, 0.4 ~ 0.6 mg.L
-1b, 0.4 ~ 0.6 mg.L
-1mn, 0.04 ~ 0.06 mg.L
-1zn, 0.01 ~ 0.03 mg.L
-1cu, 0.005 ~ 0.015 mg.L
-1mo.
Compared with prior art, the invention has the advantages that: external source crop growth regulator used in the present invention (1-amino-cyclopropane-1-carboxylic acid and methyl jasmonate) can Effective Regulation nitrate nitrogen on crop field, the distribution of underground part, thus significantly improve the ability of crop environment resistant abiotic stress, for the resistance strengthening crop provides feasible regulating measure.Be embodied in:
(1) also do not have a kind of method nutrition regulation can be coordinated mutually with abiotic stress environments regulation and control at present, this method adopts plant growth regulating substance (1-amino-cyclopropane-1-carboxylic acid and methyl jasmonate) resistance of crop can be regulated and controled combine with the distribution of nitrate nitrogen;
(2) existing technology can observe the nitrogen utilization efficiency that significantly can affect crop after crop root accumulates more nitrate nitrogen, but the distribution regulation and control not understanding Crop nitrate nitrogen can regulate the resistance of crop, this method using by plant growth regulator, the nitrate-N accumulation ratio of crop field bottom can be made to increase about 25%, significantly enhance the resistance of crop;
(3) existing technology can make crop produce more ethene and jasmonic under only knowing adverse environmental factor, but and the more ethene of unclear generation and jasmonic are to the effect of crop anti-adversity, this method utilizes spraying of ethene and jasmonate, the regulating power that the hormone produced under absolutely proving adverse environmental factor distributes crop Nitrate Nutrition and the impact on crop anti-adversity thereof;
(4), after plant exogeneous growth conditioning agent (1-amino-cyclopropane-1-carboxylic acid and methyl jasmonate) process being application of to paddy rice and rape, crop (50 ~ 150 mmolL under strong adverse growth condition are made
-1naCl, 50 ~ 200 μm of olL
-1cdCl
2) survival rate improve 30% and about 45% respectively.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the growth rate comparison diagram adding growth regulator in the embodiment of the present invention 1 under salt stress environment Yu do not add growth regulator paddy rice.
Fig. 2 is the comparison diagram of rice harves phase survival rate in the embodiment of the present invention 1.
Fig. 3 is the ratio versus figure of rice ear sprouting period underground/overground part nitrate nitrogen content in the embodiment of the present invention 1.
Fig. 4 is the comparison diagram of rice chlorophyll a content in the embodiment of the present invention 1.
Fig. 5 is the comparison diagram of paddy rice Chlorophyll in the embodiment of the present invention 1 (chlorophyll a+chlorophyll b) content.
Fig. 6 is the comparison diagram of rice dry matter amount in the embodiment of the present invention 1.
Embodiment
For the ease of understanding the present invention, hereafter will do to describe more comprehensively, meticulously to the present invention in conjunction with specification preferred embodiment, but protection scope of the present invention is not limited to following specific embodiment.
Unless otherwise defined, hereinafter used all technical terms are identical with the implication that those skilled in the art understand usually.The object of technical term used herein just in order to describe specific embodiment is not be intended to limit the scope of the invention.
Unless otherwise specified, the various raw material, reagent, instrument and equipment etc. used in the present invention are all bought by market and are obtained or prepare by existing method.
embodiment 1:
Strengthen a method for crop environment resistant abiotic stress, comprise the following steps:
The first step: crop seedling is cultivated
The cultivation of rice seedling: water planting adopts Yoshida nutrient solution, adds NH respectively in nutrient solution
4nO
3, NaH
2pO
42H
2o, K
2sO
4, CaCl
2, MgSO
47H
2o, makes macronutrient N, P, K, Ca, Mg content in nutrient solution be respectively 40,10,40,40,40 mg/L; In nutrient solution, add MnCl simultaneously
24H
2o, (NH
4)
6mo
7o
242H
2o, H
3bO
3,znSO
47H
2o, CuSO
45H
2o, FeCl
36H
2o, makes micronutrient element Mn, Mo, B, Zn, Cu, Fe content in nutrient solution be respectively 0.5,0.05,0.2,0.01,0.01,2.0 mg/L.Culture vessel is long 35cm, wide 25cm, the plastic basin of high 30 cm, above a floating expanded polystyrene sheet, thin plate has 120 holes (horizontally-arranged 12, vertical setting of types 10), 1 pocket is broadcast in every hole, 2, every pocket, be nylon wire bottom thin plate, seed is broadcast thereon, randomized arrangement.First seed is put into 50 DEG C of baking oven Dormancy breaking 3 days, vernalization in advance before sowing, the rice paddy seed after vernalization is all cultivated after 3 days with distilled water, then continues to cultivate with Yoshida nutrient solution.
The cultivation of Brassica Napus Seedling: the clorox of being soaked seed by rape seed in 20% (v/v) carries out surface sterilization 30 minutes, with distilled water flushing number all over germinateing in the earth culture of glass solarium September 30 later, Hoagland complete nutrition liquid used consist of 5 mmol.L
-1kNO
3, 1 mmol.L
-1kH
2pO
4, 7 mmol.L
-1mgSO
4, 5 mmol.L
-1ca (NO
3)
2 .4H
2o, 3 mmol.L
-1fe-EDTA, 0.5 mg.L
-1b, 0.5 mg.L
-1mn, 0.05 mg.L
-1zn, 0.02 mg.L
-1cu, 0.01 mg.L
-1mo, waters one time of nutrition liquid, waters 100 ml at every turn for every 5 days.Mean temperature 29 DEG C/19 DEG C round the clock in glass solarium, natural humidity and illumination, intensity of illumination is 205-230 Ue/ (m
2.s).
Second step: the abiotic stress process of crop
The transplanting (employing method for culturing and transplanting seedlings) of rice seedling, mid or late April, according to method nursery described in the first step, to be transplanted to after 20-25 days in the simulation pond of abiotic stress environments that (salt in simulation pond and Cd stress concentration are respectively 100 mmolL
-1naCl, 60 μm of olL
-1cdCl
2), every community 10 m
2(long 5 m, wide 2 m), transplants by the density of 6600 plants/acre, totally 3 communities, and random district group arranges.In results mid-September then.Adopt traditional waterflooding in early stage, bake the management mode of field and later stage alternation of wetting and drying mid-term.Wherein N:P
2o
5: K
2o=2:1:1, every mu of fertilizing amount is with reference to peasant habit fertilizing amount (8 kg N/ mu).Apply base manure before rice transplanting, base manure amount of nitrogen is 25% of design amount of nitrogen.
The transplanting (employing method for culturing and transplanting seedlings) of Brassica Napus Seedling, according to method nursery described in the first step before and after September 30, is transplanted in the simulation pond of abiotic stress environments that (salt in simulation pond and Cd stress concentration are respectively 100 mmolL for one month later
-1naCl, 60 μm of olL
-1cdCl
2), every community 10 m
2(long 5 m, wide 2 m), transplants by the density of 8000 plants/acre, totally 3 communities, and random district group arranges.Divide by these 5 growth periods of seedling stage, peduncle-growing period for rapeseed, flowering stage, Rape pod development phase and maturing stage, in results mid-May then.Transplant benefit after turning green seedling stage and execute urea 5 kg/ mu, optimum period, pours water and imposes a kind of sedge fertilizers for potted flowers, and spring imposes flower bud a kind of sedge fertile urea 7.5 kg/ mu in conjunction with spring irrigation.
3rd step: using of crop growth regulator
The process of the Seedling Stage paddy rice after transplanting and rape exogeneous growth conditioning agent (1-amino-cyclopropane-1-carboxylic acid and methyl jasmonate), will contain 40 μm of olL simultaneously
-11-amino-cyclopropane-1-carboxylic acid (ACC) and 100 μm of olL
-1the plant growth regulator solution of methyl jasmonate (MeJA) is sprayed onto on the blade of paddy rice and rape each at 10 in morning uniformly, the amount of spraying is as the criterion with the complete moistening all blades of growth regulating agent solution, within every 6 days from the Seedling Stage after transplanting, spray once until the pustulation period entering paddy rice terminates uniformly, and the Rape pod development phase of rape terminates.
As follows for the part test result of paddy rice:
40 μm of olL will be contained simultaneously
-11-amino-cyclopropane-1-carboxylic acid (ACC) and 100 μm of olL
-1the plant growth regulator solution of methyl jasmonate (MeJA) was sprayed onto on the blade of paddy rice uniformly each at 10 in morning, the amount of spraying is as the criterion with the complete moistening all blades of growth regulating agent solution, within 15-65 days, to sample to rice plant after treatment respectively and measures its relative growth rate; Relative growth rate (RGR)=(Ln W
f-lnW
i)/(t
f-t
i), W represents the dry weight of above-ground plant parts, and t represents the time, once samples and a rear sampling time before i and f represents respectively; Fig. 2 is the comparison of rice harves phase survival rate, salt stress and the same Fig. 1 of growth regulator process; Fig. 3 is the ratio of rice ear sprouting period underground/overground part nitrate nitrogen content, salt stress and the same Fig. 1 of growth regulator process.Difference letter wherein in Fig. 2-6 on figure post represents significant difference between different disposal.From the result of Fig. 1, plant growth regulator can significantly improve after adding paddy rice growth rate (RGR) (Fig. 1); Add the growth regulator nitrate nitrogen ratio that paddy rice underground part distributes later and add 36.9%(Fig. 3), the survival rate of paddy rice improves 33.7%(Fig. 2 simultaneously).As can be seen here, plant growth adds under abiotic environment stress conditions that plant growth regulator treatment can significantly improve the growth rate of crop, crop transplant after survival rate, and the raising of this resistance is the result because more nitrate nitrogen distributes under ground portion really.
40 μm of olL will be contained simultaneously
-11-amino-cyclopropane-1-carboxylic acid (ACC) and 100 μm of olL
-1the plant growth regulator solution of methyl jasmonate (MeJA) was sprayed onto on the blade of paddy rice uniformly each at 10 in morning, the amount of spraying is as the criterion with the complete moistening all blades of growth regulating agent solution, after 20 days, rice plant blade is carried out to the mensuration of chlorophyll content and rice plant amount of dry matter in process.Chlorophyll a and Chlorophyll (chlorophyll a+chlorophyll b) content (Fig. 4 and Fig. 5) of plant leaf blade can be significantly improved after adding plant growth regulator; The result of Fig. 6 significantly can promote plant strain growth after showing to add plant growth regulator, increases the amount of dry matter of rice plant.Fig. 4,5, the result of 6 presents the remarkable result that plant growth regulator treatment strengthens Rice Resistance abiotic stress environments more intuitively; Relative to the paddy rice of normal growth, salt stress significantly can suppress the normal growth of paddy rice, and paddy growth situation obviously improves after with the addition of plant growth regulator, this patented technology of sufficient proof is for the remarkable result improving crop environment resistant abiotic stress.
Claims (9)
1. one kind strengthens the method for crop environment resistant abiotic stress, it is characterized in that, comprise the following steps: simulation pond seedling being placed in abiotic stress environments, be spread across on the blade of seedling by the plant growth regulator dissolution homogeneity simultaneously containing 1-amino-cyclopropane-1-carboxylic acid and methyl jasmonate, the amount of sprinkling is as the criterion with the complete moistening all blades of growth regulating agent solution.
2. method according to claim 1, is characterized in that, described seedling is rice seedling and Brassica Napus Seedling.
3. method according to claim 2, it is characterized in that, the cultivation of described rice seedling comprises the following steps: water planting adopts Yoshida nutrient solution, a floating expanded polystyrene sheet above culture vessel, thin plate there are 120 holes: horizontally-arranged 12, vertical setting of types 10, 1 pocket is broadcast in every hole, 2, every pocket, it is nylon wire bottom thin plate, seed is broadcast thereon, randomized arrangement, first seed is put into 50 DEG C of baking oven Dormancy breaking 3 days before sowing, vernalization in advance, rice paddy seed after vernalization is all cultivated after 3 ~ 6 days with distilled water, continue to cultivate with Yoshida nutrient solution again.
4. method according to claim 2, it is characterized in that, the cultivation of described Brassica Napus Seedling comprises the following steps: to be soaked seed by rape seed in volume fraction be 20%, and clorox carries out surface sterilization 30 ~ 50 min, germinate in the earth culture of glass solarium for 3 ~ 6 times with distilled water flushing later, nutrient solution used is He Gelande complete nutrition liquid, within every 5 days, water one time of nutrition liquid, water 100 ml at every turn, mean temperature 29 DEG C/19 DEG C round the clock in glass solarium, natural humidity and illumination, intensity of illumination is 205 ~ 230 Ue/ (m
2.s).
5. method according to claim 2, is characterized in that, in the simulation pond of described abiotic stress environments, salt and Cd stress concentration are respectively 50 ~ 150 mmolL
-1naCl, 50 ~ 200 μm of olL
-1cdCl
2, described paddy rice is transplanted by the density of 6600 plants/acre, and described rape is transplanted by the density of 8000 plants/acre.
6. method according to claim 2, is characterized in that, the concentration for the treatment of of described 1-amino-cyclopropane-1-carboxylic acid is 10 ~ 100 μm of olL
-1, the concentration for the treatment of of methyl jasmonate is 30 ~ 200 μm of olL
-1.
7. method according to claim 2, it is characterized in that, the period of described growth regulator process is from the Seedling Stage after rice transplanting to the pustulation period, and the Seedling Stage after transplanting from rape is to the Rape pod development phase, comprise every 6 days from the Seedling Stage after transplanting and spray a plant growth regulator until the pustulation period entering paddy rice terminates uniformly, and the Rape pod development phase of rape terminates.
8. according to the method in claim 2 ~ 7 described in any one, it is characterized in that, in the Yoshida nutrient solution of described paddy rice, add NH
4nO
3, NaH
2pO
42H
2o, K
2sO
4, CaCl
2, MgSO
47H
2o, makes macronutrient N, P, K, Ca, Mg content in nutrient solution be respectively 35 ~ 45,8 ~ 12,35 ~ 45,35 ~ 4045,35 ~ 45 mg/L; In Yoshida nutrient solution, add MnCl simultaneously
24H
2o, (NH
4)
6mo
7o
242H
2o, H
3bO
3,znSO
47H
2o, CuSO
45H
2o, FeCl
36H
2o, makes micronutrient element Mn, Mo, B, Zn, Cu, Fe content in nutrient solution be respectively 0.4 ~ 0.6,0.04 ~ 0.06,0.18 ~ 0.22,0.018 ~ 0.022,0.008 ~ 0.012,1.8 ~ 2.2 mg/L.
9. according to the method in claim 2 ~ 7 described in any one, it is characterized in that, the composition of described rape He Gelande complete nutrition used liquid comprises 4 ~ 6 mmol.L
-1kNO
3, 0.5 ~ 1.5 mmol.L
-1kH
2pO
4, 6 ~ 8 mmol.L
-1mgSO
4, 4 ~ 6 mmol.L
-1ca (NO
3)
2 .4H
2o, 2 ~ 4 mmol.L
-1fe-EDTA, 0.4 ~ 0.6 mg.L
-1b, 0.4 ~ 0.6 mg.L
-1mn, 0.04 ~ 0.06 mg.L
-1zn, 0.01 ~ 0.03 mg.L
-1cu, 0.005 ~ 0.015 mg.L
-1mo.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510171234.7A CN104770231B (en) | 2015-04-13 | 2015-04-13 | It is a kind of to strengthen the method for crop environment resistant abiotic stress |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510171234.7A CN104770231B (en) | 2015-04-13 | 2015-04-13 | It is a kind of to strengthen the method for crop environment resistant abiotic stress |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104770231A true CN104770231A (en) | 2015-07-15 |
| CN104770231B CN104770231B (en) | 2017-09-15 |
Family
ID=53612259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510171234.7A Active CN104770231B (en) | 2015-04-13 | 2015-04-13 | It is a kind of to strengthen the method for crop environment resistant abiotic stress |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104770231B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107041245A (en) * | 2017-05-22 | 2017-08-15 | 湖南农业大学 | It is a kind of while improving the method that crop nitrogen utilization ratio and Cadmium resistance coerce ability |
| CN107125242A (en) * | 2017-07-03 | 2017-09-05 | 中国计量大学 | Application of 5 amino-laevulic acids in reduction Cadmium in Rice accumulation |
| CN108782202A (en) * | 2018-06-22 | 2018-11-13 | 湖南农业大学 | A method of adjusting rice leaf Determination of Chlorophyll content |
| CN108848744A (en) * | 2018-06-29 | 2018-11-23 | 华中农业大学 | Synthesis pathway inhibitor is used to enhance the new application of crop waterlogging tolerance |
| CN108934803A (en) * | 2018-04-12 | 2018-12-07 | 中国农业科学院麻类研究所 | A method of cultivating hemp seedling |
| CN109937819A (en) * | 2019-03-19 | 2019-06-28 | 湖南农业大学 | A method of the resistance to alkali ability of enhancing rice simultaneously improves iron content in rice |
| CN114982629A (en) * | 2022-06-16 | 2022-09-02 | 宁波大学 | Application of 1-aminocyclopropane carboxylic acid in promoting maturation of sporocyst branches of porphyra hyphomycete |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1224334A (en) * | 1996-07-01 | 1999-07-28 | 曾尼卡有限公司 | Plant protection method |
-
2015
- 2015-04-13 CN CN201510171234.7A patent/CN104770231B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1224334A (en) * | 1996-07-01 | 1999-07-28 | 曾尼卡有限公司 | Plant protection method |
Non-Patent Citations (11)
| Title |
|---|
| HAIWEN ZHANG 等: "The ethylene-, jasmonate-, abscisic acid- and NaCl-responsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco", 《PLANTA》 * |
| The ethylene-, jasmonate-, abscisic acid- and NaCl-responsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco;Haiwen Zhang 等;《Planta》;20041231;第220卷(第2期);第262-270页 * |
| 张丽霞 等: "乙烯调控植物耐盐性的研究进展", 《生物技术通报》 * |
| 张振华 等: "油菜生育期氮素的吸收、分配及转运特性", 《作物学报》 * |
| 张振华: "栽培条件、养分与激素对水稻耐盐性的影响及其调控机理", 《中国博士学位论文全文数据库 农业科技辑》 * |
| 栽培条件、养分与激素对水稻耐盐性的影响及其调控机理;张振华;《中国博士学位论文全文数据库 农业科技辑》;20100815(第8期);第D047-6页 * |
| 油菜生育期氮素的吸收、分配及转运特性;张振华 等;《作物学报》;20101231;第36卷(第2期);第321-326页 * |
| 王政 等: "茉莉酸和乙烯信号途径参与了油菜菌核病防卫反应", 《生物学杂志》 * |
| 茉莉酸和乙烯信号途径参与了油菜菌核病防卫反应;王政 等;《生物学杂志》;20130228;第30卷(第1期);第1-4页 * |
| 茉莉酸类物质的功能与胁迫防御;马崇坚 等;《华中农业大学学报》;20011231;第20卷(第6期);第603-608页 * |
| 马崇坚 等: "茉莉酸类物质的功能与胁迫防御", 《华中农业大学学报》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107041245A (en) * | 2017-05-22 | 2017-08-15 | 湖南农业大学 | It is a kind of while improving the method that crop nitrogen utilization ratio and Cadmium resistance coerce ability |
| CN107041245B (en) * | 2017-05-22 | 2019-12-27 | 湖南农业大学 | Method for simultaneously improving nitrogen utilization efficiency and cadmium stress resistance of crops |
| CN107125242A (en) * | 2017-07-03 | 2017-09-05 | 中国计量大学 | Application of 5 amino-laevulic acids in reduction Cadmium in Rice accumulation |
| CN108934803A (en) * | 2018-04-12 | 2018-12-07 | 中国农业科学院麻类研究所 | A method of cultivating hemp seedling |
| CN108782202A (en) * | 2018-06-22 | 2018-11-13 | 湖南农业大学 | A method of adjusting rice leaf Determination of Chlorophyll content |
| CN108848744A (en) * | 2018-06-29 | 2018-11-23 | 华中农业大学 | Synthesis pathway inhibitor is used to enhance the new application of crop waterlogging tolerance |
| CN109937819A (en) * | 2019-03-19 | 2019-06-28 | 湖南农业大学 | A method of the resistance to alkali ability of enhancing rice simultaneously improves iron content in rice |
| CN114982629A (en) * | 2022-06-16 | 2022-09-02 | 宁波大学 | Application of 1-aminocyclopropane carboxylic acid in promoting maturation of sporocyst branches of porphyra hyphomycete |
| CN114982629B (en) * | 2022-06-16 | 2024-03-26 | 宁波大学 | Application of 1-aminocyclopropane carboxylic acid in promoting maturation of aschersonia as laver filament |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104770231B (en) | 2017-09-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104770231B (en) | It is a kind of to strengthen the method for crop environment resistant abiotic stress | |
| CN102057775B (en) | Controlled-salinity cultivation method for plating crop on moderate and medium and severe saline-alkali soil | |
| CN101940147A (en) | Poplar tree cultivating method for improving saline-alkaline land | |
| CN105557492A (en) | Industrialized seedling culture method for Chinese onions | |
| CN101011019A (en) | Seedling water cultivating method by using crop water-cultivating inducing liquid | |
| CN104855222A (en) | Cultivation method capable of regulating and controlling flowering time of oriental cherry in park | |
| CN107155595A (en) | A kind of implantation methods of shed for pepper | |
| Othman et al. | Nitrogen management influenced root length intensity of young olive trees | |
| CN103283476A (en) | Slow release fertilizer seedling culture method for Larix principis-rupprechtii container seedlings | |
| CN106941903A (en) | A kind of sweet potato drip irrigation economize cultivating and growing method | |
| CN102090295A (en) | Technology for improving fruit setting rate of greenhouse fruit trees | |
| CN110249898A (en) | It is a kind of short-term training tea shoot method and its it is fast short-term training garden in application | |
| CN107258267A (en) | A kind of implantation methods of plastic tent cucumber | |
| CN103843651A (en) | Facility semi-water planting culture device and facility semi-water planting culture method for root and rhizome medicinal plants | |
| CN110839491A (en) | Plug seedling method for salvia miltiorrhiza | |
| CN102976811A (en) | Special foliar fertilizer for cold resistance of peanuts, and application method thereof | |
| CN110169323B (en) | Method for cultivating high-manganese/zinc rice seedlings to reduce cadmium content in rice | |
| CN106034693A (en) | Greenhouse rose planting technology | |
| CN102835232B (en) | Method for increasing nitrogen utilization efficiency of rape | |
| CN103262763B (en) | Plug seedling substrate and plug seedling technology using same | |
| CN107041245A (en) | It is a kind of while improving the method that crop nitrogen utilization ratio and Cadmium resistance coerce ability | |
| CN107223428A (en) | A kind of non-polluted planting method of wax gourd | |
| CN106034927A (en) | Technique for planting rose in greenhouse | |
| CN106717415A (en) | The fertilizing method that a kind of yangtse-huaihe region sweet potato High Efficient nitrogen is utilized | |
| CN110859126A (en) | Floating seedling raising method for gynostemma pentaphylla |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |