CN1047570A - 利用真空试管所进行的经离心处理的物质层测定 - Google Patents
利用真空试管所进行的经离心处理的物质层测定 Download PDFInfo
- Publication number
- CN1047570A CN1047570A CN90103726A CN90103726A CN1047570A CN 1047570 A CN1047570 A CN 1047570A CN 90103726 A CN90103726 A CN 90103726A CN 90103726 A CN90103726 A CN 90103726A CN 1047570 A CN1047570 A CN 1047570A
- Authority
- CN
- China
- Prior art keywords
- test tube
- blood
- centrifugal treating
- assembly
- blood sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 103
- 239000000463 material Substances 0.000 title claims abstract description 20
- 210000004369 blood Anatomy 0.000 claims abstract description 74
- 239000008280 blood Substances 0.000 claims abstract description 74
- 238000000034 method Methods 0.000 claims abstract description 19
- 229920003023 plastic Polymers 0.000 claims abstract description 6
- 239000011261 inert gas Substances 0.000 claims abstract description 4
- 210000003743 erythrocyte Anatomy 0.000 claims description 7
- 210000000265 leukocyte Anatomy 0.000 claims description 7
- 210000001772 blood platelet Anatomy 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 210000000601 blood cell Anatomy 0.000 claims description 3
- 238000010241 blood sampling Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 2
- 239000011521 glass Substances 0.000 abstract description 3
- 230000009969 flowable effect Effects 0.000 abstract 1
- 239000010409 thin film Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 11
- 210000002381 plasma Anatomy 0.000 description 7
- 239000000499 gel Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000009534 blood test Methods 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002313 adhesive film Substances 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000004323 axial length Effects 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000011365 complex material Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- IRXRGVFLQOSHOH-UHFFFAOYSA-L dipotassium;oxalate Chemical compound [K+].[K+].[O-]C(=O)C([O-])=O IRXRGVFLQOSHOH-UHFFFAOYSA-L 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
- B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/05—Investigating sedimentation of particle suspensions in blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/15003—Source of blood for venous or arterial blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150351—Caps, stoppers or lids for sealing or closing a blood collection vessel or container, e.g. a test-tube or syringe barrel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150374—Details of piercing elements or protective means for preventing accidental injuries by such piercing elements
- A61B5/150381—Design of piercing elements
- A61B5/150389—Hollow piercing elements, e.g. canulas, needles, for piercing the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150374—Details of piercing elements or protective means for preventing accidental injuries by such piercing elements
- A61B5/150381—Design of piercing elements
- A61B5/150473—Double-ended needles, e.g. used with pre-evacuated sampling tubes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150755—Blood sample preparation for further analysis, e.g. by separating blood components or by mixing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Ecology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- External Artificial Organs (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
一种经离心处理的物质层测定是在一个具有浮子的真空玻璃试管或透明塑料试管中进行的。发测试可能被污染过的物质时,如进行血液测试时,利用该真空试管可使血液免于直接暴露在操作技术人员面前。这种试管可以容纳大约1ml了的血液,并且充入了低压惰性气体。因为样品容量较大,所以试管和浮子的尺寸公差要求与对毛细管的尺寸公差要求相比可以宽松一些。由于在离心过程中,一种可流动的物质层会沉降到血浆层上并形成一层薄膜,所以细胞群是稳定的。
Description
本发明所涉及的是一种用来测定某种经过离心处理的物质(如血液)样品中物质层体积值的装置。这一测试是在一具有浮子的真空试管中进行的,其中的浮子可以使被测物质层得到扩张。
测定复杂物质混合物中组合层的技术是通过将该混合物样品置于具有一浮子的一毛细管或其它试管中进行离心来实现的。这个浮子最好呈圆柱状,并具有一比重,它可以使浮子沉降到离心处理后的混合物中的某一位置,在该位置上,它和试管壁间存在有一个通畅的环形体积缝隙,可以使被测组合层进入其中。这样,这些被测组分层就被处理地拉长了,从而能够更容易和更精确地测定出来。这一技术已在1977年6月7日颁布的第4,027,660号、1978年4月4日颁布的第4,082,085号、1979年5月29日颁布的第4,156,570号美国专利中作了叙述。
当被测物质可能是被污染过的物质时,如血液,通常都希望血液不要直接暴露在操作技术人员面前以便操作技术人员得到保护,当上述测定技术用毛细管来进行时,血液是暴露在操作人员面前的,这是因为毛细管是不封口的,这样,尽管在进行操作时可以采取一些通常的预防措施,被血样污染的可能性依然存在。
本发明涉及到这样一种装置,它是用来采集和测试那些可能已被污染的物质的,如血液,利用这一装置,操作人员可以免于暴露于血液。在这里,不用把血液从采集器转移到测试试管中,因此,被污染血感染的可能性就不存在了。当使用本发明中的试管和浮子时,血样的采集和测试都是在一个封闭的试管中进行的,血样一经采集后就再也不会离开试管了。本发明还有另一优点,就是在所使用的单一的封闭试管中已包含了进行细胞计数所需要的所有成分,并且这些成分都被置于一个稳定的、惰性的环境中。本发明中的试管最好使用具有一个整体密闭端的玻璃试管。它的长度与毛细管一样,但是直径要大一些以便能够容纳大约0.9ml的血液。一个圆柱状的浮子置于试管中,该浮子的外径必须要严格控制,以便能使经过离心处理的血样中的白血球细胞层和血小板层得到物理地扩张。浮子为塑性材料制成并具有一个比重,这样,当试管中的血样经过离心处理后,它便可以浮在稠密的红血球细胞当中,另外,在试管当中,还要加入一些需要的试剂,例如染色剂和红血球细胞增稠剂,最好是液状的草酸钾。试管口要用橡胶塞封闭,试管内充以低压惰性气体,低压的作用是将血样吸入试管,既可以直接从静脉血管中吸入,也可以直接通过一个双尖针头从初级血液采集装置中吸入,这一装置可以是Becton Dickinson公司的Vacutainer牌。
当从一初级血液采集试管提取病人的血样时,采集管上提供有一支针头用于将本发明中试管口部的橡胶塞刺穿,这样,采集管中的血液就会通过针头流入到测试试管中来。当血液和试管在进行离心处理时为了保存细胞群在试管中的形成,应在试管上端置入一种摇溶性的凝胶。在离心过程中,该凝胶在试管中流动落在血浆层的上面,形成了一层粘性膜。显而易见,这种凝胶的比重必须要小于血浆的比重,一种合适的选择就是硅胶。
根据本发明,当选用较大内径的试管和较大的浮子时,对于试管的内径1D和浮子的外径OD的尺寸公差要求可以比较松一些。当利用前面所述的先有技术的试管-浮子结合体来进行细胞计数时,一般都希望白血球细胞层和血小板层能够扩张十倍,当使用本发明中加大了的试管和浮子来完成此项测试时,这一十倍的扩张可以通过试管内壁和浮子间大约125μm的畅通的环形缝隙空间来获得。而用先有技术的毛细管和浮子时,只能使用大约43μm的畅通空间。这样,当测定精度为5%时,这一较大的畅通体积空间可以使尺寸公差要求放松大约三倍。
使用这种大试管和较大浮子的另一有益之处就是它可以改善细胞在白血球和血小板区域的流体动力学分布。由于存在着较大的环形缝隙空间,试管中又容纳了较大量的血样,这样,在扩张层中所存在的细胞较之先有技术的方式为多。因此,在离心过程中,血浆通过白血球和血小板层的渗透要更加方便,从而使细胞群的形成具有更明显的界限并且更加紧密。
本发明的目的就是要提供一个经过改进了的血样检验装置,它可以使操作技术人员在进行血液细胞计数时免于受到血样的污染。
本发明的进一步的目的就是要提供一个血样检验装置,其特征为在保证必需的细胞层扩张的前提下,其尺寸公差可以较为宽松。
本发明的另一个目的就是要提供一个血样检验装置,其特征为可以检验更大容量的血样。
本发明还有一个目的,就是要提供一个血样检验装置,其特征为可以使经过离心后的细胞群(Cell band)保持稳定并且不被破坏。
本发明的另一个目的就是提供一个血样检验装置,其特征为可以改善离心过程中血液细胞的渗透。
本发明的这些及其它目的和优越性通过下面结合附图对一个实施例的描叙将更为明显,其中:
图1是根据本发明的试管和浮子组件的轴向剖面图。
图2是类似于图1的一个轴向剖面图,但它显示出了这些组件是怎样用来将血样从一个初级血液采集试管吸出的;
图3为类似于图1和图2的视图,但它显示出了图1中的组件经过吸取血样和离心后的状况。
现在来看一下附图,图1中所示的是一个根据本发明制做的血液检验装置的一个较佳实施例。这一血液检验装置包括一个最好是玻璃的透明试管2,具有一整体封闭端4。试管2中有一塑料浮子6,染色剂和红血球细胞增稠剂8。一个橡胶塞10将试管开口端封闭。试管2中围绕着橡胶塞10有摇溶性凝胶12,可用一塑料圆片13来代替凝胶12,将其盖在试管2中的经过离心处理的血样上。塑料圆片13有一开口15。它的大小大约与毛细管的内径一样。试管长最好为75mm,与毛细管的长度一样,内径大约为40mm。它装载血液时的容积大约为0.9ml。浮子大约2cm长,直径大约为37.9mm。
图2所显示的是如何利用一个具有双孔针头或插管18的转移装置16将血液从一个初级血液采集试管14转移到试管2中来的。转移装置16包括一个外套管20及一个嵌在里面的针头携带塞22。针头18从塞22上的第一个凹坑24处插进,该凹坑的大小要能够容纳血液采样试管2有橡胶塞的一端进入。外套管20还有另一个凹坑26,它的大小要能够容纳初级血液采集试管14有塞子的一端进入。转移针18既要刺穿试管14的塞28,也要刺穿采样试管2的塞10。试管2中的低压使得血液通过针头18从试管14中被吸到试管2中来,直到试管2被基本充满为止。一旦充满后,将试管2从凹坑24中退出进行离心处理。将血液从一采集试管14中转移到试管2中只是将血液转移到试管2中的一种方法,十分明显的一种方法是利用一个针头直接将血液从病人身上转移到真空试管2中。
当血液进入试管2后,试剂8将与血液混合,这时,试管2将准备进行离心处理。试管2进行离心处理时,其封闭端4应向外放置,这样,经过离心处理后,红血球细胞就沉积在试管2的封闭端,而血浆则靠近试管2有橡胶塞的一端。图3显示出的就是经过离心处理后的试管2和血液的状况。红血球细胞30集中在试管2的封闭端,浮子浮在其中并凸出红血球细胞层上。白血球细胞和血小板层构成了一米黄色层32,沉积在浮子10和试管内壁之间,而血浆34则位于米黄色层和浮子10之上。摇溶性凝胶覆盖并浮在血浆层34上,这样,离心处理后,在进行细胞群厚度测量过程中,当手持试管时,经过离心处理的血液各组分层的位置便可保持不变。从而,便可将试管2置于一种前面所述的先有技术中常见的读数装置上,而不将经离心处理后的细胞群破坏。这样,就可测量出白血球细胞和血小板的轴向长度,并可通过读数装置的微处理机将其转换成细胞计数信息。
很容易看到,这项发明中的试管可用来从病人身上抽取血液,或从血液采集试管中抽取血液;然后,使可在塞好的封闭试管中直接进行血液细胞的测定,而任何人都不存在和被污染的血液接触的可能性。这样,这种血液测试过程就能够用于那些已知血液已被污染的病人,大大减少了操作者所遭受到的危险。制造试管和浮子时需遵守的尺寸公差比较宽松,同时,测试组件由于抽真空后的试管中充有一种惰性气体,所以贮存寿命较长。又由于在离心过程中摇溶性物质会在血浆上面形成一薄膜,因此当离心后手持试管时,其中所形成的各细胞层不会遭到破坏。
由于不脱离开本发明的基本思想,就可对本发明所公开的实施例进行各种各样的变化,所以,除非是后附的权利要求书中所要求的外,对上述实施例的描述并不是想要对本发明加以限制。
Claims (9)
1、一种用来对经过离心处理的装在组件中的血样进行测试的血液采样组件,血液不暴露在进行测试的人员面前,该组件包括:一支用来盛装血液的试管,该试管有一整体的端壁封闭其一端,一细长浮子位于试管中,该浮子可以沉入离心后的血样中的红血球细胞层中并可以进一步进入到血样的米黄色层中使米黄色层中的白血球细胞和血小板层得到扩张;用以与血液细胞进行反应从中增强测试效果的试剂置于该试管中,该试管与整体封闭端相对的另一端用橡胶塞密封;该试管内的压力低于大气压力以便当血液采集针头刺穿橡胶塞时血液能自动吸入到该试管中。
2、权利要求1的组件所提及的试管在用于吸取血样以前充有一种惰性气体。
3、权利要求1的组件进一步包括在所述的试管中的物质,该物质用作离心处理过程中在血样的血浆层表面形成一覆盖层,以便当离心处理结束后手持组件时使经过离心处理的血液组合层在位置上保持稳定。
4、权利要求3的组件,其中所述的形成覆盖层的物质(means)为被置于所述的试管中的一种流动性的摇溶性物质。
5、权利要求3的组件,其中所述的形成覆盖层的物质为一塑料圆片,它可以稳定在经离心处理过的血样中的血浆层表面。
6、权利要求4的组件,其中所述的试管其长度大约与常规毛细管的长度相同,其大小约能容纳0.9ml的血液。
7、一种用来对装在其中的经过离心处理的血样进行测试的血液采样组件,该组件包括:一支用来盛放血样的透明试管,该试管的一端用一个橡胶塞密封;一个位于该试管中的细长的浮子,该浮子用作将离心处理过的血样中的血液组分层物理地拉长;位于该试管中用作形成血液组分稳定覆盖层的可浮动物质(means),该覆盖层覆盖在经离心处理过的血样中的血浆层表面;具有低压的试管内腔,当橡胶塞被血液采集针刺穿后,该低压低到可使血液被自动地吸到该试管中。
8、权利要求7的组件,其中所述的可浮动物质为一种摇溶性物质,它在离心处理前放入该试管。
9、权利要求7的组件,其中所述的可浮动物质为一圆片,它可稳定在经离心处理过的血样中的血浆层表面。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35607789A | 1989-05-24 | 1989-05-24 | |
US07/356,077 | 1989-05-24 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1047570A true CN1047570A (zh) | 1990-12-05 |
CN1023153C CN1023153C (zh) | 1993-12-15 |
Family
ID=23400019
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN90103726A Expired - Fee Related CN1023153C (zh) | 1989-05-24 | 1990-05-19 | 血样检测装置 |
Country Status (15)
Country | Link |
---|---|
US (1) | US5086784A (zh) |
EP (1) | EP0399151B1 (zh) |
JP (1) | JPH0721492B2 (zh) |
CN (1) | CN1023153C (zh) |
AR (1) | AR242902A1 (zh) |
AT (1) | ATE109643T1 (zh) |
AU (1) | AU620213B2 (zh) |
BR (1) | BR9002418A (zh) |
CA (1) | CA2011100C (zh) |
DE (1) | DE69011410T2 (zh) |
DK (1) | DK0399151T3 (zh) |
ES (1) | ES2057214T3 (zh) |
FI (2) | FI100436B (zh) |
NO (1) | NO179394C (zh) |
RU (2) | RU2052186C1 (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104641230A (zh) * | 2012-07-18 | 2015-05-20 | 赛拉诺斯股份有限公司 | 快速测量小容量样本中有形血液成分的沉降率 |
CN114452684A (zh) * | 2020-11-10 | 2022-05-10 | 菲尔德森(江苏)生物技术有限公司 | 一种富血小板血浆提取分离套装及提取方法 |
Families Citing this family (53)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0774772B2 (ja) * | 1990-12-31 | 1995-08-09 | エイ. レビン ロバート | 血液サンプリング組立体、ターゲット細胞の採取方法およびターゲット成分の採取方法 |
AU644037B2 (en) * | 1992-01-21 | 1993-12-02 | Robert A. Levine | Constituent layer harvesting from a centrifuged sample in a tube |
US5342790A (en) * | 1992-10-30 | 1994-08-30 | Becton Dickinson And Company | Apparatus for indirect fluorescent assay of blood samples |
US5533518A (en) * | 1994-04-22 | 1996-07-09 | Becton, Dickinson And Company | Blood collection assembly including mechanical phase separating insert |
GB9703185D0 (en) * | 1997-02-15 | 1997-04-02 | Thurnall Plc | Individual septum capped microtubes |
US6979307B2 (en) * | 1997-06-24 | 2005-12-27 | Cascade Medical Enterprises Llc | Systems and methods for preparing autologous fibrin glue |
US5938621A (en) * | 1997-09-12 | 1999-08-17 | Becton Dickinson And Company | Collection container assembly |
US6197523B1 (en) | 1997-11-24 | 2001-03-06 | Robert A. Levine | Method for the detection, identification, enumeration and confirmation of circulating cancer and/or hematologic progenitor cells in whole blood |
US6911315B2 (en) * | 1997-11-24 | 2005-06-28 | David L. Rimm | Method for the detection, identification, enumeration and confirmation of virally infected cells and other epitopically defined cells in whole blood |
US7947236B2 (en) | 1999-12-03 | 2011-05-24 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US20030133845A1 (en) * | 2002-01-14 | 2003-07-17 | Eureka Group | Identification device for explosives or other materials |
US7179391B2 (en) * | 2002-05-24 | 2007-02-20 | Biomet Manufacturing Corp. | Apparatus and method for separating and concentrating fluids containing multiple components |
US20030205538A1 (en) * | 2002-05-03 | 2003-11-06 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
US7832566B2 (en) * | 2002-05-24 | 2010-11-16 | Biomet Biologics, Llc | Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles |
US7992725B2 (en) | 2002-05-03 | 2011-08-09 | Biomet Biologics, Llc | Buoy suspension fractionation system |
US7845499B2 (en) | 2002-05-24 | 2010-12-07 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US20060278588A1 (en) | 2002-05-24 | 2006-12-14 | Woodell-May Jennifer E | Apparatus and method for separating and concentrating fluids containing multiple components |
US7220593B2 (en) * | 2002-10-03 | 2007-05-22 | Battelle Memorial Institute | Buffy coat separator float system and method |
US7397601B2 (en) * | 2004-11-24 | 2008-07-08 | Laudo John S | Optical system for cell imaging |
US7074577B2 (en) * | 2002-10-03 | 2006-07-11 | Battelle Memorial Institute | Buffy coat tube and float system and method |
JP4609323B2 (ja) * | 2006-01-06 | 2011-01-12 | ニプロ株式会社 | 検体採取液容器 |
JP4609252B2 (ja) * | 2005-09-07 | 2011-01-12 | ニプロ株式会社 | 検体採取液容器 |
US8567609B2 (en) | 2006-05-25 | 2013-10-29 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
WO2008127639A1 (en) | 2007-04-12 | 2008-10-23 | Biomet Biologics, Llc | Buoy suspension fractionation system |
US8328024B2 (en) | 2007-04-12 | 2012-12-11 | Hanuman, Llc | Buoy suspension fractionation system |
EP2620139B1 (en) | 2008-02-27 | 2016-07-20 | Biomet Biologics, LLC | Interleukin-1 receptor antagonist rich solutions |
US8337711B2 (en) * | 2008-02-29 | 2012-12-25 | Biomet Biologics, Llc | System and process for separating a material |
CA2731156C (en) | 2008-07-21 | 2013-09-24 | Becton, Dickinson And Company | Density phase separation device |
MX339267B (es) | 2008-07-21 | 2016-05-18 | Becton Dickinson Co | Dispositivo de separacion de fase por densidad. |
AU2009274096B2 (en) | 2008-07-21 | 2012-08-02 | Becton, Dickinson And Company | Density phase separation device |
US8187475B2 (en) | 2009-03-06 | 2012-05-29 | Biomet Biologics, Llc | Method and apparatus for producing autologous thrombin |
US8313954B2 (en) * | 2009-04-03 | 2012-11-20 | Biomet Biologics, Llc | All-in-one means of separating blood components |
PL2918344T3 (pl) | 2009-05-15 | 2021-12-13 | Becton, Dickinson And Company | Urządzenie do oddzielania faz gęstości |
WO2010148135A2 (en) * | 2009-06-16 | 2010-12-23 | Levine Robert A | Harvesting target materials from centrifuged suspensions |
US9011800B2 (en) * | 2009-07-16 | 2015-04-21 | Biomet Biologics, Llc | Method and apparatus for separating biological materials |
US8591391B2 (en) | 2010-04-12 | 2013-11-26 | Biomet Biologics, Llc | Method and apparatus for separating a material |
US20120016320A1 (en) * | 2010-07-13 | 2012-01-19 | Lin Michael T | Method and Device for Liquid Delivery |
WO2012166634A2 (en) * | 2011-05-31 | 2012-12-06 | Rarecyte, Inc. | Tube and float systems for density-based fluid separation |
ES2437541B1 (es) * | 2012-07-03 | 2014-11-25 | Biotechnology Institute, I Mas D, S.L. | Dispositivo para la extracción, el almacenamiento y/o el procesado de sangre u otras sustancias de origen humano o animal, y para la aplicación de compuestos sanguíneos u otros compuestos biológicos |
US8984932B2 (en) | 2012-07-18 | 2015-03-24 | Theranos, Inc. | Rapid measurement of formed blood component sedimentation rate from small sample volumes |
US9642956B2 (en) | 2012-08-27 | 2017-05-09 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
WO2014130426A1 (en) | 2013-02-21 | 2014-08-28 | Regenerative Sciences, Llc | Blood and marrow draw processing devices and methods |
US20140271589A1 (en) | 2013-03-15 | 2014-09-18 | Biomet Biologics, Llc | Treatment of collagen defects using protein solutions |
US9950035B2 (en) | 2013-03-15 | 2018-04-24 | Biomet Biologics, Llc | Methods and non-immunogenic compositions for treating inflammatory disorders |
US9895418B2 (en) | 2013-03-15 | 2018-02-20 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
US10208095B2 (en) | 2013-03-15 | 2019-02-19 | Biomet Manufacturing, Llc | Methods for making cytokine compositions from tissues using non-centrifugal methods |
US10143725B2 (en) | 2013-03-15 | 2018-12-04 | Biomet Biologics, Llc | Treatment of pain using protein solutions |
RU2548787C1 (ru) * | 2014-04-09 | 2015-04-20 | Александр Георгиевич Рожков | Кювета для исследования коллоидных свойств жидкостных сред организма человека (варианты) |
US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
ES2593042B1 (es) * | 2015-06-03 | 2017-10-03 | Biotechnology Institute, I Mas D, S.L. | Dispositivo de recolección de sangre o de un compuesto sanguíneo |
RU176274U1 (ru) * | 2017-07-10 | 2018-01-15 | Виктор Андреевич Линд | Устройство для пробоподготовки крови для определения концентрации глюкозы в плазме крови |
WO2020154305A1 (en) | 2019-01-21 | 2020-07-30 | Eclipse Medcorp, Llc | Methods, systems and apparatus for separating components of a biological sample |
KR20220091580A (ko) | 2019-10-31 | 2022-06-30 | 이클립스 메드코프 엘엘씨 | 샘플의 구성요소들을 분리하기 위한 시스템, 방법 및 장치 |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3931018A (en) * | 1974-08-09 | 1976-01-06 | Becton, Dickinson And Company | Assembly for collection, separation and filtration of blood |
US4012325A (en) * | 1975-01-08 | 1977-03-15 | Eastman Kodak Company | Biological fluid dispenser and separator |
US4180465A (en) * | 1975-12-19 | 1979-12-25 | Sherwood Medical Industries Inc. | Fluid collection device with phase separation means |
JPS5278167A (en) * | 1975-12-19 | 1977-07-01 | Sherwood Medical Ind Inc | Liquid sampling system |
US4055501A (en) * | 1976-01-16 | 1977-10-25 | Sherwood Medical Industries Inc. | Fluid collection device with phase partitioning means |
US4088582A (en) * | 1976-01-16 | 1978-05-09 | Sherwood Medical Industries Inc. | Blood phase separation means |
US4027660A (en) * | 1976-04-02 | 1977-06-07 | Wardlaw Stephen C | Material layer volume determination |
US4235725A (en) * | 1978-08-16 | 1980-11-25 | Owens-Illinois, Inc. | Sterile blood-collecting and separating device |
DE3165646D1 (en) * | 1980-05-08 | 1984-09-27 | Terumo Corp | Apparatus for separating blood |
US4315892A (en) * | 1980-07-18 | 1982-02-16 | Sherwood Medical Industries, Inc. | Fluid collection device having phase partitioning means |
US4492634A (en) * | 1982-09-28 | 1985-01-08 | Emde Medical Research | Pre-evacuated blood collection tube with anti-hemolysis baffle system and centrifugation propelled filtration disc and efficient serum-from cells separator |
JPS6053845A (ja) * | 1983-09-05 | 1985-03-27 | Terumo Corp | 血液分離管 |
US4567754A (en) * | 1985-03-29 | 1986-02-04 | Wardlaw Stephen C | Measurement of small heavy constituent layer in stratified mixture |
SE448323B (sv) * | 1985-08-27 | 1987-02-09 | Ersson Nils Olof | Forfarande och anordnig att separera serum eller plasma fran blod |
US4774965A (en) * | 1987-07-01 | 1988-10-04 | Becton Dickinson And Co., Inc. | Material layer volume determination with correction band |
US4867887A (en) * | 1988-07-12 | 1989-09-19 | Becton Dickinson And Company | Method and apparatus for separating mononuclear cells from blood |
-
1990
- 1990-02-26 CA CA002011100A patent/CA2011100C/en not_active Expired - Fee Related
- 1990-02-27 AU AU50177/90A patent/AU620213B2/en not_active Ceased
- 1990-02-28 DK DK90103894.3T patent/DK0399151T3/da active
- 1990-02-28 ES ES90103894T patent/ES2057214T3/es not_active Expired - Lifetime
- 1990-02-28 EP EP90103894A patent/EP0399151B1/en not_active Expired - Lifetime
- 1990-02-28 AR AR90316279A patent/AR242902A1/es active
- 1990-02-28 AT AT90103894T patent/ATE109643T1/de not_active IP Right Cessation
- 1990-02-28 DE DE69011410T patent/DE69011410T2/de not_active Expired - Fee Related
- 1990-03-06 JP JP2054788A patent/JPH0721492B2/ja not_active Expired - Lifetime
- 1990-05-02 FI FI902200A patent/FI100436B/fi not_active IP Right Cessation
- 1990-05-19 CN CN90103726A patent/CN1023153C/zh not_active Expired - Fee Related
- 1990-05-23 BR BR909002418A patent/BR9002418A/pt not_active IP Right Cessation
- 1990-05-23 RU SU904743885A patent/RU2052186C1/ru active
- 1990-05-23 NO NO902289A patent/NO179394C/no unknown
- 1990-09-05 US US07/579,274 patent/US5086784A/en not_active Expired - Fee Related
-
1994
- 1994-07-01 RU RU9494024565A patent/RU2095024C1/ru active
-
1997
- 1997-05-15 FI FI972084A patent/FI972084A0/fi not_active Application Discontinuation
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104641230A (zh) * | 2012-07-18 | 2015-05-20 | 赛拉诺斯股份有限公司 | 快速测量小容量样本中有形血液成分的沉降率 |
CN104641230B (zh) * | 2012-07-18 | 2019-06-28 | 赛拉诺斯知识产权有限责任公司 | 快速测量小容量样本中有形血液成分的沉降率 |
CN114452684A (zh) * | 2020-11-10 | 2022-05-10 | 菲尔德森(江苏)生物技术有限公司 | 一种富血小板血浆提取分离套装及提取方法 |
Also Published As
Publication number | Publication date |
---|---|
CA2011100C (en) | 1996-06-11 |
DE69011410T2 (de) | 1994-12-15 |
FI972084A (fi) | 1997-05-15 |
AR242902A1 (es) | 1993-06-30 |
DK0399151T3 (da) | 1994-10-03 |
NO179394C (no) | 1996-10-02 |
DE69011410D1 (de) | 1994-09-15 |
AU620213B2 (en) | 1992-02-13 |
ATE109643T1 (de) | 1994-08-15 |
JPH02311761A (ja) | 1990-12-27 |
BR9002418A (pt) | 1991-08-06 |
CA2011100A1 (en) | 1990-11-24 |
AU5017790A (en) | 1990-11-29 |
CN1023153C (zh) | 1993-12-15 |
JPH0721492B2 (ja) | 1995-03-08 |
NO179394B (no) | 1996-06-24 |
RU94024565A (ru) | 1996-09-27 |
FI972084A0 (fi) | 1997-05-15 |
NO902289D0 (no) | 1990-05-23 |
RU2095024C1 (ru) | 1997-11-10 |
EP0399151B1 (en) | 1994-08-10 |
NO902289L (no) | 1990-11-26 |
EP0399151A1 (en) | 1990-11-28 |
ES2057214T3 (es) | 1994-10-16 |
RU2052186C1 (ru) | 1996-01-10 |
FI100436B (fi) | 1997-11-28 |
US5086784A (en) | 1992-02-11 |
FI902200A0 (fi) | 1990-05-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1023153C (zh) | 血样检测装置 | |
CN1969184B (zh) | 样本采集、处理和分析组件 | |
EP0493838B1 (en) | Constituent layer harvesting from a centrifuged sample in a tube | |
US5269317A (en) | Intravenous blood sampling apparatus | |
US3901219A (en) | Blood collecting container and method | |
US20060030051A1 (en) | Erythrocyte sedimentation rate (ESR) test measurement instrument of unitary design and method of using the same | |
US3837376A (en) | Blood serum collection tube and method | |
PL183800B1 (pl) | Urządzenie zamykające do diagnostycznego naczynia testowego lub podobnego pojemnika zwłaszcza do przeprowadzania oznaczania testu klinicznego na płynie biologicznym | |
JPH02284652A (ja) | 分析用試験管の封止栓 | |
JPH0217442A (ja) | 分離装置 | |
US4076419A (en) | Method and apparatus for hematology | |
JPS5844971B2 (ja) | 測定液を準備する方法 | |
US20060216829A1 (en) | Erythrocyte sedimentation rate (ESR) test measurement instrument of unitary design and method of using the same | |
CN216771762U (zh) | 手持式病毒检测试剂杯 | |
US20070274865A1 (en) | Erythrocyte sedimentation rate (ESR) test measurement instrument of unitary design and method of using the same | |
CN219540345U (zh) | 样本采集容器、离心组件及样本分析仪 | |
Levine et al. | A new technique for examining blood | |
CN218411922U (zh) | 一种采血试管、支架及试管组件 | |
CN216525857U (zh) | 一种新冠病毒抗体蛋白的快速测试盒 | |
Bowen et al. | 4.5 Interferences from Blood Sampling Device Materials on Clinical Assays: I Blood Collection Devices and Their Constituents and Additives | |
JP2023062899A (ja) | 血液保存容器および血液採取器具 | |
Ames et al. | An appraisal of the “Vacutainer” system for blood collection | |
JPH1096726A (ja) | 赤血球沈降速度測定管及び赤血球沈降速度の測定方法 | |
Brigden et al. | Three closed-tube methods for determining erythrocyte sedimentation rate | |
CN106821397A (zh) | 一种全自动设备真空血凝管 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |