CN104749152A - Lung cancer circulating tumor cell detection device - Google Patents
Lung cancer circulating tumor cell detection device Download PDFInfo
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- CN104749152A CN104749152A CN201510177966.7A CN201510177966A CN104749152A CN 104749152 A CN104749152 A CN 104749152A CN 201510177966 A CN201510177966 A CN 201510177966A CN 104749152 A CN104749152 A CN 104749152A
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Abstract
The invention relates to the technical field of medical equipment and particularly relates to a lung cancer circulating tumor cell detection device. The lung cancer circulating tumor cell detection device comprises a centrifuge, a fluorescent staining box, a magnetic bead separation mechanism, a display mechanism, and a machine case which is used for supporting the centrifuge, the fluorescent staining box, the magnetic bead separation mechanism and the display mechanism. According to the lung cancer circulating tumor cell detection device provided by the invention, the parts are used for detecting circulating tumor cells in blood, and the factors, which influence a diagnosis result, are few, so that a false positive medical history is not prone to occur, and furthermore, the accuracy of a detection result is effectively improved.
Description
Technical field
The present invention relates to technical field of medical instruments, especially relate to lung cancer circulating tumor cell pick-up unit.
Background technology
Lung cancer betides bronchial mucosa epithelium, the incidence of disease of immediate and mid-term lung cancer significantly increases, in some industrial big cities of American-European industrially developed country and China, lung cancer morbidity rate ranks first in male malignancy, also increase rapidly at women's incidence of disease, account for the 2nd or the 3rd of women's common cancer.Lung cancer becomes a kind of principal disease of life threatening health.In order to enable patient can obtain the effective treatment at the initial stage of morbidity, people are provided with the lung cancer detection device for vitro detection.
Lung cancer detection device in correlation technique, comprises and breathes sampling system, gas detection system, data handling system and computer control system.Wherein gas detection system and data processing and computer control system are assembled in casing jointly; Gas detection system comprises ion gun, ion-molecule reaction pipe and mass spectrometry detection system; Breathe sampling system to be connected with the ion-molecule reaction pipe of gas detection system in casing by pipeline; Ion gun is communicated with ion-molecule reaction pipe, and mass spectrometry detection system exports and is connected to data processing and computer control system.
When detecting to testee, testee pastes tight-lipped the blow gun breathing sampling system, gently gas in lung being all blown into breathes in sampling system, time remaining is greater than 7s, breathe sampling system and to be entered gas detection system in casing by gas through pipeline, the reactive ion generation ion-molecule reaction that breath produces with ionic tube in the ion-molecule reaction pipe of gas detection system becomes ion, finally by mass spectrometry detection system looks, after data processing and computer control system, provide the sick auxiliary diagnosis result such as lung cancer, fatty bar at screen.
But the lung cancer detection device in correlation technique, because testee when blowing afloat in expiration sampling system, the gas flow of breathing out and the speed of exhalation can vary with each individual, thus can ion-molecule reaction be had influence on, and then easily present false positive, cause measurement result not accurate.
Summary of the invention
The object of the present invention is to provide lung cancer circulating tumor cell pick-up unit, to solve the measurement result that exists in prior art not technical matters accurately.
Lung cancer circulating tumor cell pick-up unit provided by the invention, comprising: hydro-extractor, fluorescent dye case, Beads enrichment mechanism and indication mechanism, and for supporting the casing of hydro-extractor, fluorescent dye case, Beads enrichment mechanism and indication mechanism;
Casing upper surface is provided with well; Hydro-extractor comprises centrifuge tube and the first power source, and opening and the well of centrifuge tube are rotationally connected, and centrifuge tube lower end and the first power source are in transmission connection, and the first power source rotates for driving centrifuge tube, with separating blood sample;
Beads enrichment mechanism comprises oscillator and the first emulsion pumps, and for separating of the magnetic bead kit of lung cancer circulating tumor cell; Magnetic bead kit is arranged on the oscillator, and oscillator is used for rocking magnetic bead kit; The import of the first emulsion pumps is communicated with centrifuge tube, and the first emulsion delivery side of pump is communicated with magnetic bead kit, to be pumped in magnetic bead kit by the blood sample after separation;
Fluorescent dye case comprises injecting hole and release aperture, is provided with fluorescent dye in fluorescent dye case; Fluorescent dye case is provided with the second emulsion pumps, the import of the second emulsion pumps is communicated with release aperture, second emulsion delivery side of pump is communicated with magnetic bead kit, and the second emulsion pumps is used for fluorescent dye to be pumped in magnetic bead kit, and fluorescent dye is used for mark cycle tumour cell;
Indication mechanism comprises film clamping machine, electric expansion bar, fluorescent electronic microscope and high-flux cell image analyzers; Film clamping machine comprises the first intermediate plate, the second intermediate plate and silica gel sidewall, and the first intermediate plate and the second intermediate plate be arranged in parallel, and are provided with silica gel sidewall between the first intermediate plate and the second intermediate plate; First intermediate plate, the second intermediate plate and silica gel sidewall surround spatial accommodation; One end of electric expansion bar is arranged on casing, and the other end is connected with the edge of the second intermediate plate, for pushing the second intermediate plate to first intermediate plate;
The microscopical camera lens of fluorescent electronic is arranged on the top of the first intermediate plate, and vertical with the first intermediate plate; Fluorescent electronic microscope is electrically connected with high-flux cell image analyzers.
Further, lung cancer circulating tumor cell pick-up unit also comprises trash receptacle; Trash receptacle is arranged on the bottom of casing, and the bottom of centrifuge tube is provided with floss hole, and floss hole and trash receptacle pass through pipeline communication.
Further, the bottom surface of casing is provided with draw-in groove, the bottom of trash receptacle is provided with the projection be fixed with draw-in groove; On the sidewall of casing, on the position relative with trash receptacle, be provided with trash receptacle installing port.
Further, trash receptacle installing port is provided with waste gate; The edge of waste gate and trash receptacle installing port is hinged, to cover trash receptacle installing port.
Further, fluorescent dye is green fluorescent protein.
Further, high-flux cell image analyzers comprises display, and display is arranged on the lateral wall of casing.
Further, silica gel sidewall is also provided with vent port, for discharging the air in spatial accommodation.
Further, vent port is provided with the ventilated membrane that blocks water.
Further, oscillator being provided with the platform for supporting magnetic bead kit, platform being relatively set with two spring leafs, two spring leafs are for being fixed on platform by magnetic bead kit.
Further, on the sidewall of casing, the position relative with magnetic bead kit, is provided with kit installing port; Kit installing port is provided with kit door; The edge of kit door and kit installing port is hinged, for covering kit installing port.
Lung cancer circulating tumor cell pick-up unit provided by the invention, the red blood cell in blood sample is isolated by hydro-extractor by it, is utilizing Beads enrichment method to be separated by circulating tumor cell, by fluorescent dye mark cycle tumour cell.Circulating tumor cell after mark is pumped in clamping plate device, and the distributed image of clamping plate device Inner eycle tumour cell is obtained by fluorescent electronic microscope, by image transfer to high-flux cell image analyzers, utilize the analysis of high-flux cell image analyzers to draw the quantity of circulating tumor cell, and then judge whether testee suffers from lung cancer.Due to lung cancer circulating tumor cell pick-up unit provided by the invention, detect the circulating tumor cell in blood by above-mentioned parts, the factor affecting diagnostic result is few, so, not easily there is false-positive case history, so, effectively raise the precision of testing result.
Accompanying drawing explanation
The structural representation of the lung cancer circulating tumor cell pick-up unit that Fig. 1 provides for the embodiment of the present invention;
The structural representation of the display device in the lung cancer circulating tumor cell pick-up unit that Fig. 2 provides for the embodiment of the present invention.
Reference numeral:
1-casing; 2-hydro-extractor; 3-Beads enrichment mechanism;
4-fluorescent dye case; 5-trash receptacle; 6-indication mechanism;
11-well; 41-injecting hole; 61-film clamping machine;
62-fluorescent electronic microscope; 63-high-flux cell image analyzers;
64-electric expansion bar; 611-first intermediate plate; 612-second intermediate plate;
613-silica gel sidewall.
Embodiment
The structural representation of the lung cancer circulating tumor cell pick-up unit that Fig. 1 provides for the embodiment of the present invention; The structural representation of the display device in the lung cancer circulating tumor cell pick-up unit that Fig. 2 provides for the embodiment of the present invention; As depicted in figs. 1 and 2, the lung cancer circulating tumor cell pick-up unit that the present embodiment provides, comprise: hydro-extractor 2, fluorescent dye case 4, Beads enrichment mechanism 3 and indication mechanism 6, and for supporting the casing 1 of hydro-extractor 2, fluorescent dye case 4, Beads enrichment mechanism 3 and indication mechanism 6;
Casing 1 upper surface is provided with well 11; Hydro-extractor 2 comprises centrifuge tube and the first power source, and opening and the well 11 of centrifuge tube are rotationally connected, and centrifuge tube lower end and the first power source are in transmission connection, and the first power source rotates for driving centrifuge tube, with separating blood sample;
Beads enrichment mechanism 3 comprises oscillator and the first emulsion pumps, and for separating of the magnetic bead kit of lung cancer circulating tumor cell; Magnetic bead kit is arranged on the oscillator, and oscillator is used for rocking magnetic bead kit; The import of the first emulsion pumps is communicated with centrifuge tube, and the first emulsion delivery side of pump is communicated with magnetic bead kit, to be pumped in magnetic bead kit by the blood sample after separation;
Fluorescent dye case 4 comprises injecting hole 41 and release aperture, is provided with fluorescent dye in fluorescent dye case 4; Fluorescent dye case 4 is provided with the second emulsion pumps, the import of the second emulsion pumps is communicated with release aperture, second emulsion delivery side of pump is communicated with magnetic bead kit, and the second emulsion pumps is used for fluorescent dye to be pumped in magnetic bead kit, and fluorescent dye is used for mark cycle tumour cell;
Indication mechanism 6 comprises film clamping machine 61, electric expansion bar 64, fluorescent electronic microscope and high-flux cell image analyzers 63; Film clamping machine 61 comprises the first intermediate plate 611, second intermediate plate 612 and silica gel sidewall 613, first intermediate plate 611 and the second intermediate plate 612 and be arranged in parallel, and is provided with silica gel sidewall 613 between the first intermediate plate 611 and the second intermediate plate 612; First intermediate plate 611, second intermediate plate 612 and silica gel sidewall 613 surround spatial accommodation; One end of electric expansion bar 64 is arranged on casing 1, and the other end is connected with the edge of the second intermediate plate 612, for pushing the second intermediate plate 612 to first intermediate plate 611;
The microscopical camera lens of fluorescent electronic is arranged on the top of the first intermediate plate 611, and vertical with the first intermediate plate 611; Fluorescent electronic microscope 62 is electrically connected with high-flux cell image analyzers 63.
Wherein, the shape of casing 1 can be multiple, such as: casing 1 can be cabinet type, also can be box etc.
The material of casing 1 can be multiple, such as: stainless steel, tempered glass or plastics.
The shape of the first intermediate plate 611 and the second intermediate plate 612 can be multiple, such as: circular, square or rectangle etc.
The material of the first intermediate plate 611 and the second intermediate plate 612 can be multiple, such as: glass sheet or transparent plastic plate etc.
Well 11 is also provided with upper cover, and upper cover is for sealing well 11.In the process of centrifuge tube running, not only can reduce the loss of blood sample, blood sample can also be avoided to be subject to the pollution of impurity in external environment.
Magnetic bead kit is prior art, it is built with multiple immunomagnetic beads, and the antibody that antibody immune magnetic beads on immunomagnetic beads can be enclosed with for separating of lung cancer circulating tumor cell, such as: the antibody being separated lung cancer tumor cell in CK7/8 (cytokeratin), CK19 or EPCAM antibody (epithelial cell adhesion molecule, epithelial cell adhesion molecule) etc. prior art for Beads enrichment method.
In addition, the cross section of the sidewall of silica gel is corrugated, avoids the first intermediate plate 611 and the second intermediate plate 612 to combine like this.
In addition, the first intermediate plate 611 and the second intermediate plate 612 are installed as can for multiple, and preferably, the first intermediate plate 611 is parallel with the bottom surface of casing 1 with the second intermediate plate 612.Like this, be convenient to fluorescent electronic microscope is installed.
In the present embodiment, fluorescent electronic microscope 62 and high-flux cell image analysing computer are prior art, are no longer described in detail herein.
Lung cancer circulating tumor cell pick-up unit provided by the invention, the red blood cell in blood sample is isolated by hydro-extractor 2 by it, is utilizing Beads enrichment method to be separated by circulating tumor cell, by fluorescent dye mark cycle tumour cell.Circulating tumor cell after mark is pumped in clamping plate device, and the distributed image of clamping plate device Inner eycle tumour cell is obtained by fluorescent electronic microscope 62, by image transfer to high-flux cell image analyzers 63, utilize high-flux cell image analyzers 63 analysis to draw the quantity of circulating tumor cell, and then judge whether testee suffers from lung cancer.
Due to the lung cancer circulating tumor cell pick-up unit that the present embodiment provides, detect the circulating tumor cell in blood by above-mentioned parts, the factor affecting diagnostic result is few, so, not easily there is false-positive case history, so, effectively raise the precision of testing result.
As depicted in figs. 1 and 2, on the basis of above-described embodiment, further, lung cancer circulating tumor cell pick-up unit also comprises trash receptacle 5; Trash receptacle 5 is arranged on the bottom of casing 1, and the bottom of centrifuge tube is provided with floss hole, and floss hole and trash receptacle 5 pass through pipeline communication.
Wherein, for the ease of testing staff's manipulation, floss hole being provided with solenoid valve, when needing to discharge the waste material in centrifuge tube, opening solenoid valve.
In addition, in order to the red blood cell efficiency in blood sample can be improved, in blood sample, red blood cell lysis liquid is added.
In the present embodiment, trash receptacle 5 is arranged on the bottom of casing 1, and is positioned at the below of centrifuge tube.When after the blood sample layering in centrifuge tube, by the blood plasma (i.e. red blood cell) in blood sample, be disposed in trash receptacle 5.Like this, can avoid, in follow-up operating process, again the blood sample of layering being mixed and affecting testing result.
On the basis of above-described embodiment, further, the bottom surface of casing 1 is provided with draw-in groove, the bottom of trash receptacle 5 is provided with the projection be fixed with draw-in groove; On the sidewall of casing 1, on the position relative with trash receptacle 5, be provided with trash receptacle 5 installing port.
In the present embodiment, the bottom surface of casing 1 is provided with draw-in groove, the projection for being fixed with draw-in groove is provided with in the bottom of trash receptacle 5, and on the sidewall of casing 1, be provided with trash receptacle 5 installing port, thus be convenient to testing staff trash receptacle 5 is disassembled in casing 1, waste material timely in clear away waste bucket 5, the clean and health of holding device.
As depicted in figs. 1 and 2, on the basis of above-described embodiment, further, trash receptacle 5 installing port is provided with waste gate; The edge of waste gate and trash receptacle 5 installing port is hinged, to cover trash receptacle 5 installing port.
In the present embodiment, the edge of trash receptacle 5 installing port is hinged with waste gate, waste gate is for covering trash receptacle 5 mounting hole.When installing trash receptacle 5, by waste gate, trash receptacle 5 installing port is covered, thus make casing 1 form an airtight space, avoid the impurity in extraneous air to enter in casing 1, thus ensure the environmental health in casing 1, avoid blood sample contaminated.
On the basis of above-described embodiment, further, fluorescent dye is green fluorescent protein.
Wherein, Green Fluorescent Protein (green fluorescent protein), is called for short GFP, and this protein the earliest Shi Youxia people from village such as to be repaiied and found in the jellyfish of a kind of formal name used at school Aequorea victoria in 1962.The protein that its gene produces, under the light of blue wavelength region excites, can send green fluorescent.
In the present embodiment, utilize green fluorescent protein to mark the circulating tumor cell of blood sample, be conducive to the quantity that later stage user observes circulating tumor cell in blood sample, improve the accuracy of measurement result.
As depicted in figs. 1 and 2, on the basis of above-described embodiment, further, high-flux cell image analyzers 63 comprises display, and display is arranged on the lateral wall of casing 1.
In the present embodiment, display is arranged on the lateral wall of casing 1, is convenient to testing staff and checks and record testing result, improve the efficiency of detection.
In addition, can also be provided with printer in casing 1, printer is electrically connected with high-flux cell image analyzers 63, for testing result being printed.
On the basis of above-described embodiment, further, silica gel sidewall 613 is also provided with vent port, for discharging the air in spatial accommodation.
In the present embodiment, when the second intermediate plate 612 extrudes to the first intermediate plate 611, abut for the ease of the second intermediate plate 612 and the first intermediate plate 611, make the blood sample after marking can be laid between the first intermediate plate 611 and the second intermediate plate 612, silica gel sidewall 613 is provided with vent port, thus the air between the first intermediate plate 611 and the second intermediate plate 612 is discharged, be convenient to the first intermediate plate 611 and the second intermediate plate 612 to abut.
As depicted in figs. 1 and 2, on the basis of above-described embodiment, further, vent port is provided with the ventilated membrane that blocks water.
In the present embodiment, in order to avoid in the process of the second intermediate plate 612 to the extruding of the first intermediate plate 611, blood sample flows out and incurs loss from vent port, cause measurement result inaccurate, vent port is provided with the ventilated membrane that blocks water, to stop blood sample to flow out from vent port, improve the accuracy of measurement result.
On the basis of above-described embodiment, further, oscillator being provided with the platform for supporting magnetic bead kit, platform being relatively set with two spring leafs, two spring leafs are for being fixed on platform by magnetic bead kit.
In the present embodiment, magnetic bead kit be fixed on platform by two spring leafs, thus avoid in the process of oscillator earthquake, magnetic bead kit comes off from platform.In addition, be also convenient to testing staff and magnetic bead kit is disassembled from platform, be convenient to testing staff and clean magnetic bead kit or change magnetic bead kit.
On the basis of above-described embodiment, further, on the sidewall of casing 1, the position relative with magnetic bead kit, is provided with kit installing port; Kit installing port is provided with kit door; The edge of kit door and kit installing port is hinged, for covering kit installing port.
In the present embodiment, the sidewall of casing 1 is provided with kit installing port, more can be convenient to testing staff's dismounting and magnetic bead kit is installed.In addition, hinged at the edge of kit installing port, to cover kit installing port, thus ensure that casing 1 environment cleans, not by the impact of external environment, improve the accuracy of testing result.
Last it is noted that above each embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to foregoing embodiments to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein some or all of technical characteristic; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the scope of various embodiments of the present invention technical scheme.
Claims (10)
1. lung cancer circulating tumor cell pick-up unit, it is characterized in that, comprise: hydro-extractor, fluorescent dye case, Beads enrichment mechanism and indication mechanism, and for supporting the casing of described hydro-extractor, described fluorescent dye case, described Beads enrichment mechanism and described indication mechanism;
Described casing upper surface is provided with well; Described hydro-extractor comprises centrifuge tube and the first power source, opening and the described well of described centrifuge tube are rotationally connected, described centrifuge tube lower end and described first power source are in transmission connection, and described first power source rotates for driving described centrifuge tube, with separating blood sample;
Described Beads enrichment mechanism comprises oscillator and the first emulsion pumps, and for separating of the magnetic bead kit of lung cancer circulating tumor cell; Described magnetic bead kit is arranged on described oscillator, and described oscillator is used for rocking described magnetic bead kit; The import of described first emulsion pumps is communicated with described centrifuge tube, and described first emulsion delivery side of pump is communicated with described magnetic bead kit, to be pumped in magnetic bead kit by the blood sample after separation;
Described fluorescent dye case, to comprising injecting hole and release aperture, is provided with fluorescent dye in described fluorescent dye case; Described fluorescent dye case is provided with the second emulsion pumps, the import of described second emulsion pumps is communicated with described release aperture, described second emulsion delivery side of pump is communicated with described magnetic bead kit, described second emulsion pumps is used for described fluorescent dye to be pumped in described magnetic bead kit, and described fluorescent dye is used for mark cycle tumour cell;
Described indication mechanism comprises film clamping machine, electric expansion bar, fluorescent electronic microscope and high-flux cell image analyzers; Described film clamping machine comprises the first intermediate plate, the second intermediate plate and silica gel sidewall, and described first intermediate plate and described second intermediate plate be arranged in parallel, and are provided with described silica gel sidewall between described first intermediate plate and described second intermediate plate; Described first intermediate plate, described second intermediate plate and described silica gel sidewall surround spatial accommodation; One end of described electric expansion bar is arranged on the housing, and the other end is connected with the edge of described second intermediate plate, for pushing described second intermediate plate to described first intermediate plate;
The microscopical camera lens of described fluorescent electronic is arranged on the top of described first intermediate plate, and vertical with described first intermediate plate; Described fluorescent electronic microscope is electrically connected with described high-flux cell image analyzers.
2. lung cancer circulating tumor cell pick-up unit according to claim 1, is characterized in that, also comprise trash receptacle;
Described trash receptacle is arranged on the bottom of described casing, and the bottom of described centrifuge tube is provided with floss hole, and described floss hole and described trash receptacle pass through pipeline communication.
3. lung cancer circulating tumor cell pick-up unit according to claim 2, is characterized in that, the bottom surface of described casing is provided with draw-in groove, and the bottom of described trash receptacle is provided with the projection be fixed with described draw-in groove;
On the sidewall of described casing, on the position relative with described trash receptacle, be provided with trash receptacle installing port.
4. lung cancer circulating tumor cell pick-up unit according to claim 3, is characterized in that, described trash receptacle installing port is provided with waste gate; The edge of described waste gate and described trash receptacle installing port is hinged, to cover described trash receptacle installing port.
5. lung cancer circulating tumor cell pick-up unit according to claim 1, is characterized in that, described fluorescent dye is green fluorescent protein.
6. lung cancer circulating tumor cell pick-up unit according to claim 1, is characterized in that, described high-flux cell image analyzers comprises display, and described display is arranged on the lateral wall of described casing.
7. lung cancer circulating tumor cell pick-up unit according to claim 1, is characterized in that, described silica gel sidewall is also provided with vent port, for discharging the air in described spatial accommodation.
8. lung cancer circulating tumor cell pick-up unit according to claim 7, is characterized in that, described vent port is provided with the ventilated membrane that blocks water.
9. lung cancer circulating tumor cell pick-up unit according to claim 1, it is characterized in that, described oscillator is provided with the platform for supporting described magnetic bead kit, described platform is relatively set with two spring leafs, two described spring leafs are used for described magnetic bead kit to fix on the platform.
10. lung cancer circulating tumor cell pick-up unit according to claim 9, is characterized in that, on the sidewall of described casing, the position relative with described magnetic bead kit, is provided with kit installing port;
Described kit installing port is provided with kit door; The edge of described kit door and described kit installing port is hinged, for covering described kit installing port.
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| CN201510177966.7A CN104749152B (en) | 2015-04-15 | 2015-04-15 | lung cancer circulating tumor cell detection device |
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| CN201510177966.7A CN104749152B (en) | 2015-04-15 | 2015-04-15 | lung cancer circulating tumor cell detection device |
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| CN108660060A (en) * | 2018-08-13 | 2018-10-16 | 苏州绘真医学检验有限公司 | It is a kind of enrichment, purification cycle tumour cell micro-fluidic chip |
| CN111839594A (en) * | 2019-04-29 | 2020-10-30 | 首都医科大学附属北京胸科医院 | Lung cancer circulating tumor cell detection device |
| CN111896512A (en) * | 2020-08-10 | 2020-11-06 | 中南大学湘雅医院 | Lung cancer circulating tumor cell detection equipment |
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| CN104749152B (en) | 2018-04-27 |
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