CN110203522A - Bio-safety transfer hopper for living animal fluorescence imaging - Google Patents
Bio-safety transfer hopper for living animal fluorescence imaging Download PDFInfo
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- CN110203522A CN110203522A CN201910477729.0A CN201910477729A CN110203522A CN 110203522 A CN110203522 A CN 110203522A CN 201910477729 A CN201910477729 A CN 201910477729A CN 110203522 A CN110203522 A CN 110203522A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Evolutionary Biology (AREA)
- General Health & Medical Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
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- Zoology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses the bio-safety transfer hoppers for living animal fluorescence imaging, including transporting outer storehouse and storehouse in the transhipment outside the transhipment inside storehouse, biological protection filtration system, negative pressure maintenance system being arranged in；Storehouse is used to be filtered the air for passing in and out storehouse in the transhipment for placing living animal, the biological protection filtration system in the transhipment, and the negative pressure maintains system to be used to keep in the transhipment inside storehouse in subnormal ambient.Bio-safety transfer hopper for living animal fluorescence imaging of the invention, the common lab that is carried to of the animal safety of P3 grades of biosecurity laboratories is further detected, since many precision equipments cannot be mounted on P3 grades of laboratories, which carries the living animal detection project of high-risk viral；The present invention is equivalent to moveable P3 grades of small-sized laboratories, provides more portable means to take the transhipment of viruliferous living animal different geographical again.
Description
Technical field
The present invention relates to biological safety protection technical field, in particular to a kind of biology for living animal fluorescence imaging
Safe transit storehouse.
Background technique
Biosecurity laboratory is the essential experiment condition of research human infectious disease's virus.Have in research and lives
Property virus when, the effective protection to researcher and ambient enviroment be research basis, be the basic guarantor as scientific research
Barrier.Therefore, according to the property and the extent of injury of institute's research object, bio-safety grade (Biosafety level, BSL) is divided into
Level Four corresponds to the pathogenic microorganism of different bio-hazard degree, wherein P3 laboratory (BSL-3 or P3) and biology
Safe level Four laboratory (BSL-4 or P4) is known as high-level biosafety laboratory.Since Quaternary bio-safety laboratory is built
Difficulty is big, and expenditure of construction is high, and technical requirements are high, therefore China only discloses build up at one at present --- and Wuhan bio-safety level Four is real
Test room.Therefore a small amount of P4 grades of virus is removed, a variety of highly pathogenic viruses are studied in the laboratory P3, such as highly pathogenic fowl
Influenza virus, sars coronavirus, MERS virus, foot and mouth disease virus, encephalitis B virus, rabies viruses, prion etc. also include resistance to
The drug-resistant bacterias such as pharmacological property tubercle bacillus.It can be seen that the relevant Facilities Construction in the laboratory P3 and apparatus preparation will greatly help phase
Close the development of scientific experiment.But the laboratory P3 is not appropriate for placing large-scale precision instrument, firstly, since the laboratory P3 place has
Limit, internal structure is complicated, not only has various safety protection facilities, and need to install a large amount of basic experiment instrument, reagent, consumption
Material and biological sample, the most desirable experiment article is usually arranged in limited space properly, for placing the space of large-scale precision instrument
Deficiency, and the interior high miniature instrument of utilization rate that can generally give priority in arranging in a limited space, it is difficult to it is not high but anxious to meet utilization rate
Space needed for the large-scale instrument needed；Secondly, the laboratory P3 often carries out the air sterillizations such as hydrogen peroxide and liquid disinfection, will lead to
The damage of precision instrument accessory, is not available；Third, due to the security protection requirement of high-level biosafety laboratory, Ren Yuanjin
The strict demand of qualification and service clearance limitation out, the maintenance maintenance of large-scale instrument is difficult, causes to occur in use process each
Kind problem can not solve in time.In conclusion being also not suitable for installation large-scale precision instrument in the laboratory P3 at present, but height is caused
The research of characteristic of disease virus is but badly in need of can be realized the large-scale precision instrument of biological safety protection.
It is not widespread demand due to using large-scale instrument to study the experiment of highly pathogenic virus, market is excessively narrow, therefore
Large-scale instrument under biological safety protection does not have manufacturer to would like to do commercial development manufacture, but for national bio-safety strategy
It is to be badly in need of.According to investigation, before SARS is broken out, China's P3 lab resources are limited, scale is smaller, basic condition is poor, layout
Dispersion, lab space and usable floor area are smaller, and most of laboratory P3 can only carry out the experiment of molecular cell level, and shortage is opened
Open up the condition of animalbioassay.And the construction Jing Guo last decade, also the only laboratory small part P3 has zoopery ability,
More lack the condition of large-scale instrument installation and operation, it is difficult to meet high-end living animal experiment demand.Therefore, under present condition,
Be badly in need of developing it is a kind of can living small animal imager under the conditions of biological safety protection, to realize the dynamic of highly pathogenic virus
The experiment of object living imaging.
Currently, the biological safety protection module for thering is full-automatic biomolecule analysis system to have customization in the market, energy
Enough carry out P2 grades of molecular biology experiments.The instrument is operated by mechanical arm whole-course automation, and sample is packed by 96 orifice plates that film seals
Feeding system can carry out conventional molecular biologicals and the cell biologicals such as PCR amplification, molecular cloning, microplate reader detection, cell culture
Learn experiment.The whole external close encapsulation of experimentation, internal negative pressure system guarantee that aerosol will not be revealed, and at air outlet into
Row high efficiency filter and disinfection can be realized the biological safety protection on basis, but the system can not carry large-scale instrument, can only carry out
Molecule or cellular level experiment.In addition, also there is the aerosol toxicity contaminated system of virus-infected animal experiment in the market, some has
Corresponding biological safety protection device, some small volumes can be put into the Biohazard Safety Equipment of biosecurity laboratory, but such be set
Standby non-precision instrument, structure is simple, and component is durable, not high to environmental requirement.Bio-safety field in the market, most of instrument
Device also only meets conventional foundation experiment demand, there is no that being related to the related of high-grade, precision and advanced instrument develops and sell.Therefore, root
According to the highly pathogenic virus research specific demand of national bio-safety strategy and our unit and relevant unit, biological safe type is developed
Large-scale precision instrument is imperative, wherein design has the living small animal transfer device of biological safety protection function, Neng Gouyou
Machine by fluorescence imaging, magnetic resonance imaging (MRI), computerized tomography image (CT) and ultrasonic (ultrasound) imaging technique knot
Altogether, demand is tested to the living animal of highly pathogenic virus now to meet.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is that providing a kind of for living body
The bio-safety transfer hopper of animal fluorescence imaging.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: it is a kind of for living animal fluorescence imaging
Bio-safety transfer hopper, including transporting outer storehouse and storehouse in the transhipment outside the transhipment inside storehouse, biological protection filtering system being arranged in
System, negative pressure maintain system；
Storehouse is used for for placing living animal, the biological protection filtration system in the disengaging transhipment in the transhipment
The air in storehouse is filtered, and the negative pressure maintains system to be in subnormal ambient in the transhipment inside storehouse for keeping.
Preferably, before the outer storehouse of the transhipment includes the outer orlop plate being tightly connected, outer storehouse side plate, outer Hold Ceiling and outer storehouse
Plate offers Hatch Opening on the outer storehouse foreboard, is provided with outer door on the Hatch Opening, the first end of the outer door and described outer
Storehouse foreboard is hinged, and second end is connect by tight locking button with the outer storehouse foreboard.
Preferably, outer storehouse diaphragm is provided on the outer Hold Ceiling；Sealing strip is provided on the outer door inner wall；
Outer storehouse air inlet and the gas outlet Wai Cang are provided on the outer storehouse side plate.
Preferably, the tight locking button include be rotatably connected by pin it is different on the second end outer wall of the outer door
Shape buckle, the arc cramp being arranged on the special-shaped buckle, the handle being arranged on the special-shaped buckle and setting are in institute
It states and is used to press the baffle for keeping off the arc cramp on outer storehouse foreboard outer wall；
Disk spring is arranged on the pin, and the disk spring is between the special-shaped buckle and outer door；
The first end of the arc cramp is connect with the special-shaped buckle, and the front end face of second end is used for and the baffle
Inner wall topstick, to lock the outer door；The front end face of the arc cramp is inclination locking face, and the back side is plane, and
The thickness of the arc cramp is gradually reduced from its first end to second end.
Preferably, storehouse includes interior storehouse main body, the interior door that the interior storehouse body side is arranged in and sets in the transhipment
Set the interior storehouse diaphragm in the interior storehouse body top；
Sealing ring is provided on the inner wall of the interior door；Interior storehouse air inlet is provided in the interior storehouse main body and interior storehouse goes out
Port.
Preferably, it includes centrifugal blower, for detecting storehouse air pressure inside in the transhipment that the negative pressure, which maintains system,
B/P EGR Back Pressure Transducer EGR, circuit drives plate and power supply for controlling the centrifugal blower and B/P EGR Back Pressure Transducer EGR.
Preferably, the biological protection filtration system includes the filter installation being connected on the outer storehouse inner wall of the transhipment
Frame and the high efficiency particulate air filter group being arranged on the filter mounting rack；
The filter mounting rack includes the fixation metal plate being fixed on the outer storehouse inner wall of the transhipment and is used for the height
Effect filter group is fixed in the fixing hoop on the fixed metal plate.
Preferably, the high efficiency particulate air filter group includes that exhaust high efficiency particulate air filter, sensor high efficiency particulate air filter and air inlet are high
Imitate filter；
The exhaust high efficiency particulate air filter includes by the concatenated at least two high efficiency particulate air filter of connecting tube, and the exhaust is efficient
The inlet end of filter passes through the gas outlet Nei Cang in the first piping connection to the interior storehouse main body, the exhaust high efficiency particulate air filter
Outlet side by the second piping connection to the centrifugal blower inlet end, with to the interior storehouse main body outlet gas carry out
Filtering；The outlet side of the centrifugal blower is connected to the gas outlet Wai Cang on the outer storehouse side plate by pipeline, will be described interior
The intracorporal gas of storehouse master extracts outlet out；
The inlet end of the sensor high efficiency particulate air filter passes through the gas outlet in third piping connection to the interior storehouse main body,
Its outlet side is by the 4th piping connection to the B/P EGR Back Pressure Transducer EGR, to carry out to the gas for entering the B/P EGR Back Pressure Transducer EGR
Filter；
The inlet end of the air inlet high efficiency particulate air filter passes through the outer storehouse air inlet in the 5th piping connection to the outer storehouse side plate
Mouthful, outlet side is by the gas outlet Nei Cang in the 6th piping connection to the interior storehouse main body, to the entrance interior storehouse main body
Gas be filtered.
Preferably, the interior storehouse bottom part body is provided with several locating pieces, be provided on the outer orlop plate it is several with
The corresponding positioning mounting plate of the locating piece.
Preferably, location hole is provided in the positioning mounting plate, the location hole includes by the positioning mounting plate
The conical pilot hole and cylindricality location hole that surface successively opens up downwards, the side of the positioning mounting plate is offered vertically through extremely
The locking hole of the cylindricality location hole；Movable in the locking hole to be inserted with locking post, the outer end of the locking post is provided with
Chuck, is provided with pressure spring between the chuck and the side wall of the positioning mounting plate, the inner end of the locking post is provided with spherical shape
Face, the inner end of the locking post extend into the cylindricality positioning hole；
The bottom of the locating piece is connected with for plugging the positioning column in the location hole, on the positioning column along with
Its axially vertical direction offers jack, is provided on the side of the inner end towards the locking post of the positioning column bottom
Inclined guide surface；
After the positioning column is inserted into the location hole, the inner end cooperation of the locking post is inserted into the positioning column
In jack, to lock the positioning column.
The beneficial effects of the present invention are:
Bio-safety transfer hopper for living animal fluorescence imaging of the invention, so that P3 grades of biosecurity laboratories
The common lab that is carried to that animal is capable of safety is further detected, since many precision equipments cannot be mounted on P3 grades in fact
Room is tested, which carries the living animal detection project of high-risk viral；
The present invention is equivalent to moveable P3 grades of small-sized laboratories, to take viruliferous living animal different geographical again
Transhipment provides more portable means.The present invention is by being arranged diaphragm matched with fluorescence imaging device, so that carrying high
The living body fluorescent of virus of endangering toy is possibly realized.Storehouse can be carried out Fast Installation in transporting outer storehouse in transhipment of the invention
And positioning, it is easy to use；The present invention can provide the energy that lasting life maintains gas to living animal in carrying transportational process
Power；And can outlet to device and air inlet be filtered by High-cfficiency Filtration equipment, guarantee biological protection safety.
Detailed description of the invention
Fig. 1 is the schematic diagram of internal structure of the bio-safety transfer hopper for living animal fluorescence imaging of the invention；
Fig. 2 is the external structure schematic diagram of the bio-safety transfer hopper for living animal fluorescence imaging of the invention；
Fig. 3 is the structural schematic diagram of tight locking button of the invention；
Fig. 4 is the cross-sectional view of tight locking button of the invention；
Fig. 5 is the structural schematic diagram in storehouse in transhipment of the invention；
Fig. 6 is the structural schematic diagram of biological protection filtration system of the invention；
Fig. 7 is the structural schematic diagram of high efficiency particulate air filter group of the invention；
Fig. 8 is the structural schematic diagram of outer orlop plate of the invention；
Fig. 9 is the structural schematic diagram of the overlook direction of positioning mounting plate of the invention；
Figure 10 is the structural schematic diagram of the side-looking direction of locating piece of the invention；
Figure 11 is the structural schematic diagram at another side visual angle of locating piece of the invention；
Figure 12 is the structural schematic diagram for looking up direction of interior storehouse main body of the invention；
Figure 13 is the cross-sectional view of positioning mounting plate of the invention；
Figure 14 is the structural schematic diagram of locating piece and positioning mounting plate cooperation of the invention.
Description of symbols:
1-outer the storehouse of transhipment；10-outer orlop plates；11-outer storehouse side plates；12-outer Hold Ceilings；13-outer storehouse foreboards；14—
Outer door；15-outer storehouse diaphragms；16-outer storehouse air inlets；17-the gas outlets Wai Cang；The first end of 18-outer doors；19-is outer
The second end of door；
2-transport interior storehouse；20-interior storehouse main bodys；21-interior doors；22-interior storehouse diaphragms；23-interior storehouse air inlets；
24-the gas outlets Nei Cang；25-lock fasteners；
3-biological protection filtration systems；30-filter mounting racks；31-high efficiency particulate air filter groups；32-efficient the mistakes of exhaust
Filter；33-sensor high efficiency particulate air filters；34-air inlet high efficiency particulate air filters；300-fixed metal plates；301-fixing hoops；
310-the first pipeline；311-the second pipeline；312-connecting tubes；313-third pipelines；314-the four pipeline；315-the five
Pipeline；316-the six pipeline；
4-negative pressure maintain system；40-centrifugal blowers；41-B/P EGR Back Pressure Transducer EGRs；42-circuit drives plates；43-power supply electricity
Source；
5-tight locking buttons；50-special-shaped buckles；51-arc cramps；52-pins；53-disk springs；54-baffles；
55-handles；56-inclination locking faces；57-mounting blocks；
6-locating pieces；60-positioning columns；61-jacks；62-inclined guide surfaces；
7-positioning mounting plates；70-location holes；71-conical pilot holes；72-cylindricality location holes；73-locking holes；
74-locking posts；75-chucks；76-pressure springs；77-sphericals.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded one or more
The presence or addition of a other elements or combinations thereof.
The purpose of bio-safety transfer hopper for living animal fluorescence imaging of the invention will carry high-risk viral
Living animal safety takes P3 grades of biosecurity laboratories out of, to carry out scientific research on corresponding fluorescence detection device.
So the function that the present apparatus has includes at least: 1, providing lasting life to living animal in carrying transportational process and maintain
The ability of gas；2, negative pressure is persistently provided in carrying transportational process, to guarantee with the breathed generation of virulent living animal
Aerosol will not diffuse to the external world；3, the outlet of device and air inlet are filtered by High-cfficiency Filtration equipment；4, equipment top layer
Equipped with enhanced window piece, fluorescence is enable to be irradiated to internal living animal, guarantees the realization of fluorescence imaging function.In conjunction with following reality
Example is applied, is described in detail.
As represented in figures 1 through 14, a kind of bio-safety transfer hopper for living animal fluorescence imaging of the present embodiment, including
It transports outer storehouse 1 and is arranged in and transport storehouse 2 in the transhipment inside outer storehouse 1, biological protection filtration system 3, negative pressure maintenance system 4；
Storehouse 2 is for placing living animal, air of the biological protection filtration system 3 for storehouse 2 in transporting to disengaging in transporting
It is filtered, negative pressure maintains system 4 to be in subnormal ambient in transporting inside storehouse 2 for keeping.
Transporting outer storehouse 1 includes outer orlop plate 10, outer storehouse side plate 11, outer Hold Ceiling 12 and the outer storehouse foreboard 13 being tightly connected
(can be attached by welding, guarantee there is good air-tightness), offers Hatch Opening on outer storehouse foreboard 13, is provided on Hatch Opening
Outer door 14, the first end 18 of outer door and outer storehouse foreboard 13 are hinged, and second end is connected by tight locking button 5 and outer storehouse foreboard 13
It connects.
Outer storehouse diaphragm 15 is provided on outer Hold Ceiling 12, due in fluorescence detection exciting light and reflected light require through
Cross outer storehouse diaphragm 15 enter transport in storehouse 2, in a preferred embodiment, outer storehouse diaphragm 15 may be selected tempering treated high
Purity K9 glass, and externally storehouse diaphragm 15 carries out the antireflective process of 300-1200nm wavelength, guarantees fluorescence imaging function
Realization；K9 glass also can guarantee integrality when safety warehouse carries out high pressure steam sterilization.It is provided on outer 14 inner wall of door close
Strip of paper used for sealing, outer door 14 are able to maintain good leakproofness when closing.It is provided with outer storehouse air inlet 16 on outer storehouse side plate 11 and outer storehouse goes out
Port 17.In a preferred embodiment, interface flange can be set on outer storehouse side plate 11, outer storehouse air inlet is set in interface flange
The mouth gas outlet 17 16 and Wai Cang (npt threaded hole can be used), is then tightly connected using mating tracheae.
In one embodiment, referring to Fig. 2-4, tight locking button 5 includes being rotatably connected by pin 52 the of outer door
Special-shaped buckle 50, the arc cramp 51 being arranged on special-shaped buckle 50, the handle being arranged on special-shaped buckle 50 on two ends, 19 outer wall
Hand 55 and the baffle 54 for being used to press arc blocking shape cramp 51 being arranged on outer 13 outer wall of storehouse foreboard；Special-shaped buckle 50 passes through installation
Block 57 is connected on 19 outer wall of second end of outer door, disk spring 53 is arranged on pin 52, and disk spring 53 is in different
Between shape buckle 50 and mounting blocks 57；The first end of arc cramp 51 is connect with special-shaped buckle 50, and the front end face of second end is used
In the inner wall topstick with baffle 54, to lock outer door 14；The front end face of arc cramp 51 is inclination locking face 56, and the back side is
Plane, and the thickness of arc cramp 51 is gradually reduced from its first end to second end.
Wherein, tight locking button 5, will be under handle 55 when opening outer door 14 referring to Fig. 2 for realizing the locking of outer door 14
Pressure rotates special-shaped buckle 50, rotates counterclockwise the arc cramp 51 of special-shaped 50 right end of buckle, is detached from baffle 54, opens outer
Door 14；It closes outer door 14 and carries out inverse operation, arc cramp 51 rotates clockwise, and arc cramp 51 is inserted into baffle 54 and outside
In the locking slot formed between storehouse foreboard 13,51 front end face of arc cramp is inclination locking face 56, and lower end is thin, behind upper end, from
And be inserted into locking slot convenient for arc cramp 51, the elastic force effect of disk spring 53 is outside by special-shaped buckle 50 and arc cramp 51
Press, to compress outer door 14, realizes outer storehouse so that the front end face of arc cramp 51 and the inner wall of baffle 54 be made to keep pressing
The locking of door 14.
Storehouse 2 includes interior storehouse main body 20, the interior door 21 that interior 20 side of storehouse main body is arranged in and is arranged in interior storehouse master in transporting
The interior storehouse diaphragm 22 at 20 top of body.Wherein interior storehouse main body 20 due to directly with take viruliferous living animal and contact, in order to protect
It demonstrate,proves closed reliability and preferably takes overall processing；It is solid with ultraviolet glue and sealant between interior storehouse diaphragm 22 and interior storehouse main body 20
It sets, same interior storehouse diaphragm 22 also carries out the anti-reflection processing of 300-1200nm wavelength and handles with tempering.Subsequent carry out fluorescence imaging
When, fluorescence imaging device is arranged above outer storehouse diaphragm 15, and fluorescence is through outer storehouse diaphragm 15, interior storehouse diaphragm 22 to interior storehouse
Living animal in main body 20 is imaged.
Interior door 21 is buckled with interior storehouse main body 20 by tight locking button 5 25 lockings, such as butterfly；It is set on the inner wall of interior door 21
It is equipped with sealing ring, guarantees sealing；It is provided with interior storehouse air inlet 23 in interior storehouse main body 20 and (npt spiral shell can be used in the gas outlet Nei Cang 24
Pit), then it is tightly connected again by mating tracheae.
Negative pressure maintain system 4 include centrifugal blower 40, the B/P EGR Back Pressure Transducer EGR 41 for detect interior 2 air pressure inside of storehouse of transhipment,
For controlling the circuit drives plate 42 and power supply 43 of centrifugal blower 40 and B/P EGR Back Pressure Transducer EGR 41.
Power supply 43 is preferably phosphoric acid lithium battery, can maintain centrifugal blower 40 and B/P EGR Back Pressure Transducer EGR in the handling process
41 normal work, and can charge.Circuit drives plate 42 mainly realizes fan speed regulation, voltage conversion, B/P EGR Back Pressure Transducer EGR 41
The functions such as ad conversion, guarantee that entire negative pressure maintains the steady operation of system 4.In one embodiment, when in interior storehouse main body 20
Outer relative pressure can make blower increase air quantity when being higher than -50pa, reduce air quantity when negative pressure is more than -100pa.B/P EGR Back Pressure Transducer EGR 41
Section uses small-sized monocrystalline silicon differential pressure transmitter or its modified version, carries out work by comparing the extraneous pressure difference with interior storehouse
Make.40 major function of centrifugal blower guarantees interior storehouse negative pressure, so that the air in interior storehouse only passes through gas outlet and is discharged, guarantees biological protection
Stability, while in realizing ventilation volume 20 times or so per hour inside storehouse, to meet the working stamndard of biological protection.
Biological protection filtration system 3 includes being connected to the filter mounting rack 30 transported on outer 1 inner wall of storehouse and being arranged in mistake
High efficiency particulate air filter group 31 on filter mounting rack 30；Filter mounting rack 30 includes being fixed in the fixation transported on outer 1 inner wall of storehouse
Metal plate 300 and fixing hoop 301 for being fixed in high efficiency particulate air filter group 31 on fixed metal plate 300.
High efficiency particulate air filter group 31 includes exhaust high efficiency particulate air filter 32, sensor high efficiency particulate air filter 33 and air inlet high efficiency particulate air filter
34；The Nang Shi filter with efficient-filtering function can be selected in high efficiency particulate air filter therein.
Being vented high efficiency particulate air filter 32 includes passing through the concatenated at least two high efficiency particulate air filter of connecting tube 312, wherein exhaust system
Installation biological protection requires to need to carry out bipolar high efficiency filter, and exhaust high efficiency particulate air filter 32 includes concatenated 2 in the present embodiment
High efficiency particulate air filter.The inlet end of exhaust high efficiency particulate air filter 32 is gone out by the interior storehouse that the first pipeline 310 is connected in interior storehouse main body 20
The outlet side of port 24, exhaust high efficiency particulate air filter 32 is connected to the inlet end of centrifugal blower 40 by the second pipeline 311, with internal
The gas of 20 outlet of storehouse main body is filtered；The outlet side of centrifugal blower 40 is connected to the outer storehouse on outer storehouse side plate 11 by pipeline
Out outlet is extracted the gas in interior storehouse main body 20 in gas outlet 17；
The inlet end of sensor high efficiency particulate air filter 33 is connected to the gas outlet in interior storehouse main body 20 by third pipeline 313,
Its outlet side is connected to B/P EGR Back Pressure Transducer EGR 41 (can connect by pagoda connector) by the 4th pipeline 314, to pass into negative pressure
The gas of sensor 41 is filtered；
The inlet end of air inlet high efficiency particulate air filter 34 is connected to the outer storehouse air inlet on outer storehouse side plate 11 by the 5th pipeline 315
16, outlet side is connected to the gas outlet Nei Cang 24 in interior storehouse main body 20 by the 6th pipeline 316, to the interior storehouse main body 20 of entrance
Gas be filtered.Sustain life movable air and anesthesia gas are provided for the living animal in interior storehouse main body 20.Wherein, it is
Guarantee that the reliability of biological protection, the service life of high efficiency particulate air filter are no more than 20 times.
Interior 20 bottom of storehouse main body is provided with several locating pieces 6 (being 3 in the present embodiment), if being provided on outer orlop plate 10
Do positioning mounting plate 7 corresponding with locating piece 6.Cooperated by locating piece 6 with positioning mounting plate 7 and realizes that transporting interior storehouse 2 is transporting
Fast Installation and positioning in outer storehouse 1 guarantee the stabilization of position in 2 placement process of storehouse in transporting, when to carry out fluorescence imaging
The determination of initial position.
In one embodiment, referring to Fig. 8-14, be provided with location hole 70 in positioning mounting plate 7, location hole 70 include by
The conical pilot hole 71 and cylindricality location hole 72 that 7 surface of positioning mounting plate successively opens up downwards, the side of positioning mounting plate 7 opens up
Have vertically through the locking hole 73 to cylindricality location hole 72；It is movable in locking hole 73 to be inserted with locking post 74, locking post 74
Outer end is provided with chuck 75, and pressure spring 76, the inner end setting of locking post 74 are provided between chuck 75 and the side wall of positioning mounting plate 7
There is spherical 77, the inner end of locking post 74 is extend into cylindricality location hole 72.71 diameter of conical pilot hole is greater than cylindricality location hole
72, it is oriented to for the insertion to positioning column 60, can make to position positioning column 60 and some deviations of cylindricality location hole 72
Column 60 is smoothly inserted into.
Wherein, the bottom of locating piece 6 is connected with for plugging the positioning column 60 in location hole 70, on positioning column 60 along with
Its axially vertical direction offer not through jack 61, on the side of the inner end towards locking post 74 of 60 bottom of positioning column
It is provided with inclined guide surface 62.
Wherein, after positioning column 60 is inserted into location hole 70, the inner end cooperation of locking post 74 is inserted into inserting for positioning column 60
In hole 61, with locking positioning column 60.
Inclined guide surface 62 is used to cooperate with the spherical 77 of the inner end of locking post 74, determines when positioning column 60 is inserted into cylindricality
When the hole 72 of position, inclined guide surface 62 squeezes spherical 77, and locking post 74 is made to move right, and is inserted into positioning column 60 smoothly；When fixed
After position column 60 is inserted into bottom, under the elastic force effect of pressure spring 76, locking post 74 is moved to the left, to be inserted into inserting for positioning column 60
In hole 61, with locking positioning column 60.
Such as Figure 13, in the initial state, pressure spring 76 is former length, and in the raw, locking post 74 is inserted into cylindricality to the left
A distance in location hole 72, but look up from Vertical Square, the spherical 77 of 74 left end of locking post is also in inclined guide surface 62
Right side enables positioning column 60 to be successfully inserted in cylindricality location hole 72, and the right side of inclined guide surface 62 can press spherical
77, so as to which locking post 74 is squeezed to the right.In a preferred embodiment, 62 the top of inclined guide surface is connected by arcwall face
To jack 61, slid into jack 61 conducive to locking post 74 from 62 the top of inclined guide surface.Interior storehouse main body 20 is installed to and is turned
When transporting in outer storehouse 1, referring to Fig.1 4, by determining in the outer storehouse 1 of the alignment transhipment of positioning column 60 on the locating piece 6 of interior 20 bottom of storehouse main body
Location hole 70 on the mounting plate 7 of position, is inserted into, conical pilot hole 71 is oriented to, the inclined guide surface of 60 bottom of positioning column
62 contact and squeeze spherical 77, so that locking post 74 is moved right, positioning column 60 is made to be successfully inserted at bottom, at this time in pressure spring 76
Under elastic force effect, locking post 74 is moved to the left, and is just smoothly slid into the jack 61 of positioning column 60, with locking positioning column 60,
Interior storehouse main body 20 is set to keep stablizing；To realize the quick positioning and installation of interior storehouse main body 20, and it is able to maintain locking.It can be square
Just the determination of initial position when fluorescence imaging is carried out, it is steady in subsequent progress fluorescence imaging to be also able to maintain interior 20 position of storehouse main body
It is fixed.During transportation, further secured to guarantee, locating piece 6 and positioning mounting plate 7 can be fixed by screw.When
Need by interior storehouse main body 20 from transport in outer storehouse 1 take out when, first pulling chuck 75 is by locking post 74 from the jack 61 of positioning column 60
Then middle extraction carries away interior storehouse main body 20 upwards.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (10)
1. a kind of bio-safety transfer hopper for living animal fluorescence imaging, which is characterized in that including transporting outer storehouse and setting
Storehouse, biological protection filtration system, negative pressure maintain system in transhipment outside the transhipment inside storehouse；
Storehouse is used for for placing living animal, the biological protection filtration system to storehouse in the disengaging transhipment in the transhipment
Air is filtered, and the negative pressure maintains system to be in subnormal ambient in the transhipment inside storehouse for keeping.
2. the bio-safety transfer hopper according to claim 1 for living animal fluorescence imaging, which is characterized in that described
Transporting outer storehouse includes outer orlop plate, outer storehouse side plate, outer Hold Ceiling and the outer storehouse foreboard being tightly connected, and is opened up on the outer storehouse foreboard
There is Hatch Opening, outer door is provided on the Hatch Opening, the first end of the outer door and the outer storehouse foreboard are hinged, and second end is logical
Tight locking button is crossed to connect with the outer storehouse foreboard.
3. the bio-safety transfer hopper according to claim 2 for living animal fluorescence imaging, which is characterized in that described
Outer storehouse diaphragm is provided on outer Hold Ceiling；Sealing strip is provided on the outer door inner wall；It is provided on the outer storehouse side plate
Outer storehouse air inlet and the gas outlet Wai Cang.
4. the bio-safety transfer hopper according to claim 2 for living animal fluorescence imaging, which is characterized in that described
Tight locking button includes that special-shaped buckle, setting are rotatably connected on the second end outer wall of the outer door by pin in the abnormity
Arc cramp on buckle, the handle being arranged on the special-shaped buckle and being arranged on the outer storehouse foreboard outer wall is used for
Pressure keeps off the baffle of the arc cramp；
Disk spring is arranged on the pin, and the disk spring is between the special-shaped buckle and outer door；
The first end of the arc cramp connect with the special-shaped buckle, the front end face of second end for in the baffle
Wall topstick, to lock the outer door；The front end face of the arc cramp is inclination locking face, and the back side is plane, and described
The thickness of arc cramp is gradually reduced from its first end to second end.
5. the bio-safety transfer hopper according to claim 3 for living animal fluorescence imaging, which is characterized in that described
Storehouse includes interior storehouse main body, the interior door that the interior storehouse body side is arranged in and is arranged in the interior storehouse body top in transporting
Interior storehouse diaphragm；
Sealing ring is provided on the inner wall of the interior door；Interior storehouse air inlet and interior storehouse outlet are provided in the interior storehouse main body
Mouthful.
6. the bio-safety transfer hopper according to claim 5 for living animal fluorescence imaging, which is characterized in that described
Negative pressure maintain system include centrifugal blower, for detecting the B/P EGR Back Pressure Transducer EGR of storehouse air pressure inside in the transhipment, for controlling
State the circuit drives plate and power supply of centrifugal blower and B/P EGR Back Pressure Transducer EGR.
7. the bio-safety transfer hopper according to claim 6 for living animal fluorescence imaging, which is characterized in that described
Biological protection filtration system includes the filter mounting rack being connected on the outer storehouse inner wall of the transhipment and is arranged in the filter
High efficiency particulate air filter group on mounting rack；
The filter mounting rack includes the fixation metal plate being fixed on the outer storehouse inner wall of the transhipment and is used for the efficient mistake
Filter group is fixed in the fixing hoop on the fixed metal plate.
8. the bio-safety transfer hopper according to claim 7 for living animal fluorescence imaging, which is characterized in that described
High efficiency particulate air filter group includes exhaust high efficiency particulate air filter, sensor high efficiency particulate air filter and air inlet high efficiency particulate air filter；
The exhaust high efficiency particulate air filter includes by the concatenated at least two high efficiency particulate air filter of connecting tube, the exhaust high efficiency filter
The inlet end of device is gone out by the gas outlet Nei Cang in the first piping connection to the interior storehouse main body, the exhaust high efficiency particulate air filter
Gas end, to the inlet end of the centrifugal blower, was carried out by the second piping connection with the gas to the interior storehouse main body outlet
Filter；The outlet side of the centrifugal blower is connected to the gas outlet Wai Cang on the outer storehouse side plate by pipeline, by the interior storehouse
Main intracorporal gas extracts outlet out；
The inlet end of the sensor high efficiency particulate air filter is gone out by the gas outlet in third piping connection to the interior storehouse main body
Gas end is by the 4th piping connection to the B/P EGR Back Pressure Transducer EGR, to be filtered to the gas for entering the B/P EGR Back Pressure Transducer EGR；
The inlet end of the air inlet high efficiency particulate air filter passes through the outer storehouse air inlet in the 5th piping connection to the outer storehouse side plate,
Outlet side is by the gas outlet Nei Cang in the 6th piping connection to the interior storehouse main body, to the gas for entering the interior storehouse main body
It is filtered.
9. the bio-safety transfer hopper according to claim 1 for living animal fluorescence imaging, which is characterized in that described
Interior storehouse bottom part body is provided with several locating pieces, and several positioning peaces corresponding with the locating piece are provided on the outer orlop plate
Loading board.
10. the bio-safety transfer hopper according to claim 9 for living animal fluorescence imaging, which is characterized in that institute
It states and is provided with location hole in positioning mounting plate, the location hole includes the cone successively opened up downwards by the location and installation plate surface
Shape pilot hole and cylindricality location hole, the side of the positioning mounting plate are offered vertically through the locking to the cylindricality location hole
Hole；Movable in the locking hole to be inserted with locking post, the outer end of the locking post is provided with chuck, the chuck and described fixed
It is provided with pressure spring between the side wall of position mounting plate, the inner end of the locking post is provided with spherical, and the inner end of the locking post is stretched
Enter to the cylindricality positioning hole；
The bottom of the locating piece is connected with for plugging the positioning column in the location hole, edge and its axis on the positioning column
Jack is offered to vertical direction, is provided with inclination on the side of the inner end towards the locking post of the positioning column bottom
Guide surface；
After the positioning column is inserted into the location hole, the inner end cooperation of the locking post is inserted into the jack of the positioning column
In, to lock the positioning column.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910477729.0A CN110203522B (en) | 2019-06-03 | 2019-06-03 | Biological safety transfer bin for live animal fluorescence imaging |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910477729.0A CN110203522B (en) | 2019-06-03 | 2019-06-03 | Biological safety transfer bin for live animal fluorescence imaging |
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| CN110203522B CN110203522B (en) | 2020-07-31 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112998660A (en) * | 2021-03-01 | 2021-06-22 | 无锡博医光电技术有限公司 | Biological safety bin for small animal living body fluorescence imaging instrument and fluorescence imaging instrument |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101497052B (en) * | 2008-12-09 | 2010-08-18 | 天津市食品加工工程中心 | Double-flow three-dimensional temperature gradient regulating box |
| CN201997339U (en) * | 2010-10-20 | 2011-10-05 | 中国人民解放军军事医学科学院微生物流行病研究所 | Biological safety protection cabin |
| CN104406283B (en) * | 2014-12-15 | 2017-04-26 | 苏州兴亚净化工程有限公司 | Cuboidal high-efficiency air supply outlet |
| CN104523385A (en) * | 2014-12-31 | 2015-04-22 | 珠海新康杰特种车辆有限公司 | Full-negative pressure laminar flow efficient disinfecting infectious disease care ambulance |
| CN205912514U (en) * | 2016-08-03 | 2017-02-01 | 杨基汉 | Ventilation blower mouth |
| CN206740586U (en) * | 2017-04-27 | 2017-12-12 | 王健 | A kind of outdoor environmental quality detection device |
| CN208267680U (en) * | 2018-02-26 | 2018-12-21 | 苏州高欣净化科技有限公司 | Bio-safety pass-through box |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112998660A (en) * | 2021-03-01 | 2021-06-22 | 无锡博医光电技术有限公司 | Biological safety bin for small animal living body fluorescence imaging instrument and fluorescence imaging instrument |
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