CN104736726A - 直接核酸扩增试剂盒、试剂和方法 - Google Patents

直接核酸扩增试剂盒、试剂和方法 Download PDF

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Publication number
CN104736726A
CN104736726A CN201380055445.3A CN201380055445A CN104736726A CN 104736726 A CN104736726 A CN 104736726A CN 201380055445 A CN201380055445 A CN 201380055445A CN 104736726 A CN104736726 A CN 104736726A
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CN
China
Prior art keywords
nucleic acid
amplification
cyclodextrin
reagent composition
dried reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201380055445.3A
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English (en)
Chinese (zh)
Inventor
J.K.霍尔顿
P.J.塔特内尔
K.L.拉梅顿
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GE Healthcare UK Ltd
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GE Healthcare UK Ltd
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Publication date
Application filed by GE Healthcare UK Ltd filed Critical GE Healthcare UK Ltd
Priority to CN202110196732.2A priority Critical patent/CN112899354A/zh
Publication of CN104736726A publication Critical patent/CN104736726A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201380055445.3A 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法 Pending CN104736726A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110196732.2A CN112899354A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1219137.5 2012-10-24
GB201219137A GB201219137D0 (en) 2012-10-24 2012-10-24 Direct nucleic acid amplification kit, reagent and method
US13/799800 2013-03-13
US13/799,800 US20140113294A1 (en) 2012-10-24 2013-03-13 Direct nucleic acid amplification kit, reagent and method
PCT/EP2013/072206 WO2014064169A1 (en) 2012-10-24 2013-10-23 Direct nucleic acid amplification kit, reagent and method

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202110196732.2A Division CN112899354A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法

Publications (1)

Publication Number Publication Date
CN104736726A true CN104736726A (zh) 2015-06-24

Family

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Family Applications (2)

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CN202110196732.2A Pending CN112899354A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法
CN201380055445.3A Pending CN104736726A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法

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CN202110196732.2A Pending CN112899354A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法

Country Status (7)

Country Link
US (2) US20140113294A1 (enExample)
EP (1) EP2912191B1 (enExample)
JP (2) JP2015533507A (enExample)
CN (2) CN112899354A (enExample)
GB (1) GB201219137D0 (enExample)
IN (1) IN2015DN02793A (enExample)
WO (1) WO2014064169A1 (enExample)

Cited By (2)

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Publication number Priority date Publication date Assignee Title
CN106755414A (zh) * 2016-12-23 2017-05-31 宁波海尔施基因科技有限公司 一种检测dna遗传标记的方法
CN111593112A (zh) * 2020-05-12 2020-08-28 深圳市星蝶科技有限公司 一种用于检测β-地中海贫血的PCR试剂及试剂盒

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GB201219137D0 (en) 2012-10-24 2012-12-05 Ge Healthcare Uk Ltd Direct nucleic acid amplification kit, reagent and method
JP5904153B2 (ja) * 2013-03-29 2016-04-13 ソニー株式会社 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ
US11987836B2 (en) 2014-09-30 2024-05-21 Global Life Sciences Solutions Usa Llc Method for nucleic acid analysis directly from an unpurified biological sample
US11008604B2 (en) 2014-12-18 2021-05-18 Global Life Sciences Solutions Operations UK Ltd Analyte detection on a solid support by nucleic acid amplification coupled to an immunoassay
US10822645B1 (en) 2014-12-23 2020-11-03 Global Life Sciences Solutions Operations UK Ltd Methods and reagents for reverse-transcription polymerase chain reaction
WO2016106111A1 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
WO2016106113A1 (en) * 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
GB201515355D0 (en) * 2015-08-28 2015-10-14 Ge Healthcare Ltd A method and kit for analyte detection
WO2017086394A1 (ja) * 2015-11-18 2017-05-26 国立研究開発法人海洋研究開発機構 標的核酸の定量方法及びそのためのキット
GB201602880D0 (en) 2016-02-18 2016-04-06 Ge Healthcare Uk Ltd Method and composition for biomolecule stabilization
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
EP4491721A3 (en) * 2017-05-19 2025-03-05 Gen-Probe Incorporated Dried compositions containing flap endonuclease
AU2018300000B2 (en) * 2017-07-10 2021-11-11 Gen-Probe Incorporated Analytical systems and methods for nucleic acid amplification using sample assigning parameters
JP7555858B2 (ja) * 2020-03-11 2024-09-25 花王株式会社 皮膚表上脂質由来ｒｎａの調製方法
JP2022060807A (ja) * 2020-10-05 2022-04-15 キヤノンメディカルシステムズ株式会社 乾燥試薬、試薬キット、乾燥試薬の製造方法、分析方法及び核酸増幅方法
AU2022239609A1 (en) * 2021-03-19 2023-10-05 Becton, Dickinson And Company Isothermal amplification of pathogens
CN114317700B (zh) * 2021-12-28 2024-01-30 无锡科智达科技有限公司 荧光pcr试剂冻干保护剂、冻干方法及应用
WO2025049920A1 (en) * 2023-09-01 2025-03-06 Biomeme, Inc. Methods and compositions for sample processing

Citations (4)

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US5565318A (en) * 1994-09-02 1996-10-15 Pharmacia Biotech, Inc. Room temperature stable reagent semi-spheres
US5705345A (en) * 1991-01-10 1998-01-06 Amersham International Plc Methods and kits for preparing nucleic acids using cyclodextrin
CN101516337A (zh) * 2006-09-18 2009-08-26 通用电气医疗集团生物科学公司 用玻璃覆盖的生物学试剂的制备
WO2010066908A1 (en) * 2008-12-12 2010-06-17 Eurogentec S.A. Use of cyclodextrins to improve the specificity, sensitivity and yield of nucleic acid amplification reactions

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US5972386A (en) * 1995-12-19 1999-10-26 Flinders Technologies Pty, Ltd. Dry solid medium for storage and analysis of genetic material
US5593824A (en) * 1994-09-02 1997-01-14 Pharmacia Biotech, Inc. Biological reagent spheres
US7122304B2 (en) * 1997-05-12 2006-10-17 Whatman, Inc. Methods for the storage and synthesis of nucleic acids using a solid support
EP1053311B1 (en) * 1998-02-02 2007-11-28 QIAGEN North American Holdings, Inc. Compositions and methods for using a lysing matrix for isolating dna
WO2004007078A1 (en) * 2002-07-12 2004-01-22 British Biocell International Limited Lateral flow assay device and method
US7462475B2 (en) * 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
AU2007297484B2 (en) * 2006-09-18 2013-07-18 Global Life Sciences Solutions Usa Llc Preparation of glassified biological reagents
US8153401B2 (en) * 2007-05-17 2012-04-10 Applied Biosystems, Llc Method for direct amplification from crude nucleic acid samples
SI2294225T1 (sl) * 2008-06-30 2015-04-30 Life Technologies Corporation Postopek za neposredno amplifikacijo iz vzorcev surove nukleinske kisline
CN102018672B (zh) * 2010-11-29 2013-09-18 广州朗圣药业有限公司 一种水溶性药物的脂质体冻干组合物及其制备方法
GB201219137D0 (en) 2012-10-24 2012-12-05 Ge Healthcare Uk Ltd Direct nucleic acid amplification kit, reagent and method
FR2998845B1 (fr) * 2012-12-04 2016-04-01 Autoliv Dev Generateur de gaz

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5705345A (en) * 1991-01-10 1998-01-06 Amersham International Plc Methods and kits for preparing nucleic acids using cyclodextrin
US5565318A (en) * 1994-09-02 1996-10-15 Pharmacia Biotech, Inc. Room temperature stable reagent semi-spheres
CN101516337A (zh) * 2006-09-18 2009-08-26 通用电气医疗集团生物科学公司 用玻璃覆盖的生物学试剂的制备
WO2010066908A1 (en) * 2008-12-12 2010-06-17 Eurogentec S.A. Use of cyclodextrins to improve the specificity, sensitivity and yield of nucleic acid amplification reactions
CN102317472A (zh) * 2008-12-12 2012-01-11 欧洲基因技术股份有限公司 使用环糊精改进核酸扩增反应的特异性、灵敏度和产率

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106755414A (zh) * 2016-12-23 2017-05-31 宁波海尔施基因科技有限公司 一种检测dna遗传标记的方法
CN106755414B (zh) * 2016-12-23 2020-09-01 宁波海尔施基因科技有限公司 一种检测dna遗传标记的方法
CN111593112A (zh) * 2020-05-12 2020-08-28 深圳市星蝶科技有限公司 一种用于检测β-地中海贫血的PCR试剂及试剂盒

Also Published As

Publication number Publication date
IN2015DN02793A (enExample) 2015-09-04
EP2912191B1 (en) 2019-09-04
US10907201B2 (en) 2021-02-02
JP2018183156A (ja) 2018-11-22
GB201219137D0 (en) 2012-12-05
JP7009011B2 (ja) 2022-02-10
EP2912191A1 (en) 2015-09-02
WO2014064169A1 (en) 2014-05-01
US20140113294A1 (en) 2014-04-24
JP2015533507A (ja) 2015-11-26
US20170152546A1 (en) 2017-06-01
CN112899354A (zh) 2021-06-04

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Application publication date: 20150624

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