CN104692872A - Conditioning liquid and method for promoting two-time mushroom production of pleurotus eryngii - Google Patents

Conditioning liquid and method for promoting two-time mushroom production of pleurotus eryngii Download PDF

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CN104692872A
CN104692872A CN201510065119.1A CN201510065119A CN104692872A CN 104692872 A CN104692872 A CN 104692872A CN 201510065119 A CN201510065119 A CN 201510065119A CN 104692872 A CN104692872 A CN 104692872A
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pleurotus eryngii
fruiting
conditioning liquid
mushroom
bacterium bag
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CN104692872B (en
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谈峰
许建民
张健
李玉娟
李敏
徐建平
王莹
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Jiangsu Yanjiang Agricultural Science Research Institute
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Jiangsu Yanjiang Agricultural Science Research Institute
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Abstract

The invention provides a conditioning liquid and a method for promoting two-time mushroom production of pleurotus eryngii. In each 1,000 ml of an aqueous solution, the conditioning liquid comprises the components by weight: 17-20 mg of VB9, 85-107 mg of KH2PO4, 75-100 mg of MgSO4, 55-80 mg of pure peptone, and 90-120 mg of urea. According to the conditioning liquid and the method for promoting two-time mushroom production of pleurotus eryngii provided by the invention, wet mushrooms of the pleurotus eryngii are picked and then are produced again, so that the commercial yield is multiplied, the reasonable benefit of factory-like cultivation of the pleurotus eryngii can still be maintained after the market price fluctuates greatly, and the favorable sustainable development of the industry can be ensured.

Description

Promote conditioning liquid and the method for Pleurotus eryngii secondary fruiting
Technical field
The present invention relates to cultivation planting material and the cultivating method of a kind of edible mushrooms, specifically, relate to a kind of conditioning liquid and the method that promote Pleurotus eryngii secondary fruiting.
Background technology
Pleurotus eryngii has another name called pleurotus eryngii, is south of europe, the colory large-scale agaric of one of the northern and Central Asia's high mountain in Africa, grassland, desert region.Meat is plump, and quality is tender and crisp, and has almond flavor, is high-grade edible mushrooms, the very high edible and physiotherapy function had.
The business cultivation mode of current employing is mainly factory culture, and fruiting pattern is a damp fruiting system, and fresh mushroom production level is biological yield 42%.But along with production-scale continuous expansion, Pleurotus eryngii is lowered one's standard or status final as the popular consumer's goods by high-grade mushroom, and price is tending towards popular, really move towards popular dining table, and the yield level under existing cultivation mode can not maintain the existence of enterprise.
Summary of the invention
The present invention is intended to solve one of technical problem in correlation technique at least to a certain extent.For this reason, one object of the present invention is to propose a kind of conditioning liquid promoting Pleurotus eryngii secondary fruiting, and it can make Pleurotus eryngii after a fruiting, then secondary fruiting.
The present invention completes based on the following discovery of contriver:
Contriver finds, under existing factory culture condition, after having plucked a damp mushroom, through rational artificial regulatory, can go out a damp mushroom again, thus make commercial quantities double.
Thus, according to an aspect of the present invention, the invention provides a kind of conditioning liquid promoting Pleurotus eryngii secondary fruiting, every 1000ml aqueous solution comprises:
According to embodiments of the invention, in described conditioning liquid, also comprise in every 1000ml aqueous solution: growth regulator 18-30mg.
Wherein, described growth regulator is obtained by Pleurotus eryngii mushroom handle chip (abbreviation tankage) lixiviate.Specifically by tankage and water by 1: 1 weight ratio mix, and add appropriate 95% alcohol-pickled, filtering supernatant obtains.According to embodiments of the invention, every 10 kilograms of tankage can be selected to pulverize, and add water 10 kilograms, 95% medical alcohol 500ml, soaks 24 hours, filters to obtain supernatant liquor and obtain described growth regulator.
In a second aspect of the present invention, the invention provides aforementioned conditioning liquid and promoting the purposes in Pleurotus eryngii secondary fruiting.
In a third aspect of the present invention, the invention provides a kind of method promoting Pleurotus eryngii secondary fruiting, comprise the following steps:
(1) in the Pleurotus eryngii bacterium bag after a fruiting, the aforementioned conditioning liquid making Pleurotus eryngii secondary fruiting is inputted;
(2) under described bacterium bag being placed in the condition being suitable for fruiting, to obtain the Pleurotus eryngii of secondary fruiting.
According to embodiments of the invention; the mode that conditioning liquid inputs slowly with low discharge described in step (1) inputs; be specially and input with the flow of 30-35mL/s; preferred 33.4mL/s; the effective absorption of liquid in bacterium bag being conducive to like this inputting also farthest protects mycelium to be hurt less; accelerate bacterium bag to recover, effectively can supplement the physiology water content needed for the growth of Pleurotus eryngii normal growth thus, to recover mycelia vigor as early as possible.
According to embodiments of the invention, after the described conditioning liquid of input, regulate in the step (1) water content in described bacterium bag to 50-55%.The mycelial vigor of bacterium bag effectively can be regulated thus to best, reach optimum secondary fruiting demand.
According to embodiments of the invention, also comprise after hand breaks the first damp mushroom of described Pleurotus eryngii of gathering step (1) is front, clear up described bacterium bag surface.Normally used lancinating is adopted mushroom method and is changed hand into and break and adopt mushroom method by the present invention, so both can improve and adopt mushroom speed, can keep again the complete of bacterium bag mouth.Preferably, described cleaning described bacterium bag surface comprises: dead mushroom flower bud and the scab mycoderma of clearing up described bacterium bag surface in conjunction with Sao bacterium, can clean bacterium block top layer thus.Wherein, Sao bacterium is removed for the bacterium block old mycoderma in surface and upper tide are remained mushroom body, and stimulates bacterium bag top layer mycelia to transform from nourishing and growing to reproductive growth.
According to embodiments of the invention, the condition being suitable for fruiting described in step (2) comprises: cultivation 5-7 days under regulation and control temperature 17-22 DEG C, mushroom room, relative air humidity 70-85%, shading rate 80-90% condition, the growth of described bacterium bag can be nursed one's health thus, recover mycelia vigor.
According to embodiments of the invention, the condition being suitable for fruiting described in step (2) also comprises: regulate temperature 10-14 DEG C, mushroom room, relative air humidity 87-95%, intensity of illumination 450-1500 lux, CO 2concentration is 0.5%-0.7%, keeps 1-3 days, urges flower bud to make Pleurotus eryngii.Preferably, the described mushroom room temperature during urging flower bud is compared with the described mushroom room temperature during cultivation in step (2), and reduce 8-12 DEG C, contriver is surprised to find that, is more conducive to like this urge flower bud.
According to embodiments of the invention, the condition being suitable for fruiting described in step (2) comprises further: reduce the relative air humidity in mushroom room when mushroom flower bud starts to expand to 75-85%, CO 2concentration adjustment is to 0.8-2.2%, and temperature controls at 13-18 DEG C, keeps 5-7 days, until the sporophore of described Pleurotus eryngii secondary fruiting is gathered.Wherein, CO 2concentration adjustment specifically grows early stage by CO mushroom flower bud 2concentration progressively regulates and controls to 2.2% by 0.8%, and keep 1-2 days, mushroom flower bud Later growth is again by CO 2concentration progressively returns control to 0.8%.
Existing Pleurotus eryngii industrial cultivation pattern only goes out a damp mushroom, second damp mushroom is not because output and proterties reach the market demands of commodity mushroom and be not worth, the cultivating method making Pleurotus eryngii secondary fruiting provided by the invention, the high-quality mushroom making Pleurotus eryngii go out a tide again after having plucked a damp mushroom to meet market demands, commercial quantities can be made double, thus after large fluctuation appears in market value, the factory culture of Pleurotus eryngii still can be kept to have rational profit, and then guarantee the optimum Sustainable development of industry.
Embodiment
Embodiment described below is exemplary, is intended to for explaining the present invention, and can not be interpreted as limitation of the present invention.
In following examples, water used all meets GB5749 regulation.
Embodiment 1
1. the preparation of conditioning liquid
Every 1000ml aqueous solution comprises:
2. two fruiting steps:
Former mushroom room, after having plucked a damp mushroom, cleans mushroom room, sterilizes.
In former mushroom room, after rejecting useless bacterium bag, still successively place bacterium bag by latticed.
2.1 bacterium bag requirements
Require that bacterium bag is without miscellaneous bacteria and insect pest, mycelia color and luster is pure white, and bacterium block is healthy.
2.2 bacterium bag conditionings
(1) mycelium stimulation: remove the residual mushroom root on bacterium bag surface, old mycoderma, remove imperfect attachment thing.
(2) fluid infusion: input the conditioning liquid prepared, conditioning liquid input speed is 33.4mL/s, in conjunction with supplementary bacterium bag physiology water, is supplemented to bacterium bag culture material relative water content 50%-55%.
(3) cultivation: gained bacterium is wrapped in 18-22 DEG C, relative air humidity 70-85%, shading rate 80-90% condition under cultivate 5 ~ 7 days.
2.3 urge flower bud
When oneself existing white fluffy mycelium of bacterium bag top layer 50%, increase relative air humidity to 90% ~ 95%, adjust the temperature to 10-14 DEG C, intensity of illumination 450-1500Lx, CO 2concentration is 0.5%-0.7%, keeps 1-2 days, reduces and ventilates.Wherein, the mushroom room temperature during urging flower bud is compared with the described mushroom room temperature during cultivation, and reduction amplitude controls at 8-12 DEG C.
2.4 dredge flower bud
Often bag stays the large flower bud that 2-3 growing way is good.
2.5 management of producing mushroom
(1) temperature: mushroom flower bud growth (1-3 days) control temperature in early stage, between 13-16 DEG C, remains unchanged afterwards.
(2) humidity: mushroom flower bud growth (1-3 days) relative air humidity in early stage remains on 90%-95%, and the middle and later periods, (4-7 days) was progressively reduced to 75%-80% when namely mushroom flower bud starts to expand.
(3) CO 2concentration
Regulation and control mushroom room CO 2concentration, at 0.8%-2.2%, specifically grows early stage by CO mushroom flower bud 2concentration progressively regulates and controls to 2.2% by 0.8%, and keep 1-2 days, mushroom flower bud Later growth (5-7 days) is again by CO 2concentration progressively returns control to 0.8%.
(4) illumination
Mushroom flower bud growth beforehand control mushroom room intensity of illumination is at 500-800Lx, and later stage 300-500Lx, until the sporophore of Pleurotus eryngii secondary fruiting is gathered.
The mean yield of final gained bacterium bag is each bacterium amount of contracting for fixed output quotas is 170 grams, and its sub-entities is: the long 8cm of handle, diameter 3cm, mushroom lid diameter 4.5cm.
Embodiment 2
1. the preparation of conditioning liquid
Every 1000ml aqueous solution comprises:
Wherein, described growth regulator is obtained by Pleurotus eryngii mushroom handle chip (abbreviation tankage) lixiviate.Specifically pulverized by every 10 kilograms of tankage, add water 10 kilograms, 95% medical alcohol 500ml, soaks 24 hours, filters to obtain supernatant liquor and obtain described growth regulator.
2. two fruiting steps:
Through the cultivation management with embodiment 1 the same terms.
The mean yield of final gained bacterium bag is each bacterium amount of contracting for fixed output quotas is 276 grams, and its sub-entities is: the long 14.7cm of handle, diameter 5.6cm, mushroom lid diameter 6.1cm.
Comparing embodiment 1
Except not adding conditioning liquid of the present invention, adopt the cultivation management with embodiment 1 the same terms.The mean yield of final gained bacterium bag is each bacterium amount of contracting for fixed output quotas is 65 grams, and its sub-entities is: the long 5.1cm of stem, diameter 2.4cm, cap 3.2cm.
Can be found out by comparing embodiment, the obtain after the conditioning liquid of the application of the invention second damp mushroom, output and proterties are obviously better than the second damp mushroom not using conditioning liquid to obtain.
In the description of this specification sheets, specific features, structure, material or feature that the description of reference term " embodiment ", " some embodiments ", " example ", " concrete example " or " some examples " etc. means to describe in conjunction with this embodiment or example are contained at least one embodiment of the present invention or example.In this manual, to the schematic representation of above-mentioned term not must for be identical embodiment or example.And the specific features of description, structure, material or feature can combine in one or more embodiment in office or example in an appropriate manner.In addition, when not conflicting, the feature of the different embodiment described in this specification sheets or example and different embodiment or example can carry out combining and combining by those skilled in the art.
Although illustrate and describe embodiments of the invention above, be understandable that, above-described embodiment is exemplary, can not be interpreted as limitation of the present invention, and those of ordinary skill in the art can change above-described embodiment within the scope of the invention, revises, replace and modification.

Claims (10)

1. promote a conditioning liquid for Pleurotus eryngii secondary fruiting, it is characterized in that, every 1000ml aqueous solution comprises:
2. the conditioning liquid of promotion Pleurotus eryngii secondary fruiting according to claim 1, is characterized in that, also comprises growth regulator 18-30mg in described every 1000ml aqueous solution.
3. conditioning liquid according to claim 1 is promoting the purposes in Pleurotus eryngii secondary fruiting.
4. promote a method for Pleurotus eryngii secondary fruiting, it is characterized in that, comprising:
(1) in the Pleurotus eryngii bacterium bag after a fruiting, conditioning liquid according to claim 1 is inputted;
(2) under described bacterium bag being placed in the condition being suitable for fruiting, to obtain the Pleurotus eryngii of secondary fruiting.
5. method according to claim 4, is characterized in that, described in step (1), conditioning liquid inputs with the flow of 30-35mL/s.
6. method according to claim 4, is characterized in that, in the step (1) after the described conditioning liquid of input, regulates water content in described bacterium bag to 50-55%.
7. method according to claim 4, is characterized in that, also comprises after hand breaks the first damp mushroom of described Pleurotus eryngii of gathering step (1) is front, clears up described bacterium bag surface.
8. method according to claim 4, is characterized in that, the condition being suitable for fruiting described in step (2) comprises: cultivation 5-7 days under regulation and control temperature 17-22 DEG C, mushroom room, relative air humidity 70-85%, shading rate 80-90% condition.
9. method according to claim 4, is characterized in that, the condition being suitable for fruiting described in step (2) also comprises: regulate temperature 10-14 DEG C, mushroom room, relative air humidity 87-95%, intensity of illumination 450-1500Lx, CO 2concentration is 0.5%-0.7%, keeps 1-3 days, urges flower bud to make Pleurotus eryngii.
10. method according to claim 4, is characterized in that, the condition being suitable for fruiting described in step (2) also comprises: reduce the relative air humidity in mushroom room when mushroom flower bud starts to expand to 75-85%, CO 2concentration is 0.8-2.2%, and temperature controls at 13-18 DEG C, keeps 5-7 days.
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CN107162753A (en) * 2017-06-14 2017-09-15 广东东阳光药业有限公司 The culture medium for cultivating and method of Phlebopus portentosus
CN107602261A (en) * 2017-10-27 2018-01-19 广西浙缘农业科技有限公司 A kind of multiple fruiting nutritional supplementation liquid of pleurotus eryngii and preparation method thereof
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Publication number Priority date Publication date Assignee Title
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CN107602261A (en) * 2017-10-27 2018-01-19 广西浙缘农业科技有限公司 A kind of multiple fruiting nutritional supplementation liquid of pleurotus eryngii and preparation method thereof
CN111066569A (en) * 2019-12-18 2020-04-28 江苏沿江地区农业科学研究所 Method for producing pleurotus eryngii with fresh and fragrant taste
CN111066569B (en) * 2019-12-18 2021-11-05 江苏沿江地区农业科学研究所 Method for producing pleurotus eryngii with fresh and fragrant taste

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