CN104641762A - Method for breaking period of dormancy of trollius chinensis seeds - Google Patents
Method for breaking period of dormancy of trollius chinensis seeds Download PDFInfo
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- CN104641762A CN104641762A CN201510063373.8A CN201510063373A CN104641762A CN 104641762 A CN104641762 A CN 104641762A CN 201510063373 A CN201510063373 A CN 201510063373A CN 104641762 A CN104641762 A CN 104641762A
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- trollflower
- sodium carbonate
- seed
- gibberellin
- chinensis seeds
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Abstract
The invention discloses a method for breaking the period of dormancy of trollius chinensis seeds. The method comprises the following steps: (1) screening out impurities and husks of trollius chinensis seeds harvested in the same year, washing by warm water, and airing for later use; (2) filling a cotton cloth pocket with the treated trollius chinensis seeds, sealing, putting into a refrigerator, and storing at low temperature; (3) weighing 1000g of to-be-treated trollius chinensis seeds, and pouring 2 liters of water into a barrel and reserving for later use; (4) preparing a gibberellic acid sodium carbonate solution for later use; (5) pouring the prepared gibberellic acid sodium carbonate solution into the water, and blending uniformly; (6) then pouring the trollius chinensis seeds into warm water containing the gibberellic acid sodium carbonate solution, and stirring; (7) fishing out the trollius chinensis seeds after four days, washing clearly, and airing; (8) preparing a potassium permanganate solution for later use; and (9) soaking the trollius chinensis seeds by the potassium permanganate solution for 4 hours, fishing out and airing. The method is simple, and by adopting the operation method, the characteristics of short cycle, high sprouting rate and the like can be realized.
Description
Technical field
The present invention relates to seeds cultivation technical field, relate to a kind of method breaking the trollflower seed dormancy phase specifically.
Background technology
Trollflower is Ranunculaceae herbaceos perennial, with flower as being used as medicine, has effect of clearing heat and detoxicating, anti-inflammation, skin maintenance, enhancing body immunity.The primary raw material of jinlianhua pian, globeflower capsule, trollius chinensis granular, tropaeolum oral liquid is exactly trollflower.Totall effective rate in clinical treatment reaches 92.7%.Trollflower is also widely used in the aspects such as health care, beauty treatment, flowers, environmental protection.There is higher medicine, economy and the ecological value.Along with globeflower medicinal materials demand increases day by day, wild resource reserves reduce year by year, and trollflower becomes endangered plants at present.Develop with nature, socio-intangible asset for realizing trollflower industry, improve trollflower grows wild environment, trollflower large area artificial cultivation is imperative.Promote trollflower artificial cultivation technique so only have, carry out standardized planting and industrialization production, the market demand that trollflower increases fast could be met.
The key technology of artificial cultivation trollflower is how to break the trollflower seed dormancy phase.At present, main employing is collected the mode adding low temperature treatment for a long time and is broken the trollflower seed dormancy phase, and research shows lily feet seed room temperature dry Tibetan again through 1 month low temperature treatment after 6-9 month, and get final product breaking dormancy and germinate, germination rate is 47%.Therefore there is cycle long, the feature such as germination rate is low in prior art, still has larger room for improvement.
Summary of the invention
Technical purpose of the present invention is to provide a kind of simple to operate, the cycle is short, germination rate is high method breaking the trollflower seed dormancy phase
Technical solution of the present invention is as follows:
A kind of method breaking the trollflower seed dormancy phase of the present invention, comprises the steps:
1) by the trollflower screen for seeds decontamination ghost of gathering then, warm water is eluriated, and dries, for subsequent use;
2) load in cotton pocket, sealing, puts into refrigerator Cord blood, and take out to spring in the coming year, room temperature places 1 day;
3) get 1000 grams of pending trollflower seeds, and get 2 premium on currency and pour in bucket for subsequent use, regulate water temperature to 20 DEG C-25 DEG C;
4) gibberellin sodium carbonate liquor is prepared for subsequent use;
5) the gibberellin sodium carbonate liquor modulated is poured in the water of step 3), mix well;
6) then by step 2) the trollflower seed of gained stirs in pouring containing gibberellin sodium carbonate liquor warm water into;
7) after 4 days, trollflower seed is pulled out and rinse clean, dry;
8) liquor potassic permanganate is prepared for subsequent use;
9) liquor potassic permanganate of the trollflower seed step 8) gained of step 7) gained is soaked 4 hours, pull out and dry.
Wherein, in described step 1), the temperature of warm water is 30-40 DEG C.
Wherein, described step 2) in temperature in refrigerator be-2 DEG C--5 DEG C.
Wherein, in described step 4), the compound method of gibberellin sodium carbonate liquor is, gets 100 grams of sodium carbonate and 20 grams of gibberellin are dissolved in 1 premium on currency.
Wherein, in described step 6), the time of stirring is no less than half an hour.
Wherein, indoor temperature 20 DEG C-25 DEG C is kept in described step 6).
Wherein, in described step 8, then the preparation method of liquor potassic permanganate dissolves 5 grams of potassium permanganate in 2 premium on currency.
The invention has the beneficial effects as follows: step of the present invention comprise warm water process, refrigeration, more first after through mycin sodium carbonate liquor and liquor potassic permanganate process, have that work is simple, easy to operate, germination rate high.
[embodiment]
Describe the present invention below in conjunction with specific embodiment, following embodiment and explanation are only used for explaining the present invention, but not as a limitation of the invention.
embodiment 1:
By the trollflower screen for seeds decontamination ghost of gathering then, eluriate with 35 DEG C of warm water, dry, for subsequent use.
Loaded by trollflower seed in cotton pocket, sealing, put into refrigerator (controlling refrigerator temperature for-3 DEG C) and preserve, take out to spring in the coming year, room temperature is placed 1 day; Get 1000 grams of pending trollflower seeds, and get 2 premium on currency and pour in bucket for subsequent use, regulate water temperature to 20 DEG C; Preparation gibberellin sodium carbonate liquor is for subsequent use, is specially to get 100 grams of sodium carbonate and 20 grams of gibberellin are dissolved in 1 premium on currency.In the water that the gibberellin sodium carbonate liquor modulated is poured into, mix well; Then trollflower seed is poured in the warm water containing gibberellin sodium carbonate liquor and be stirred to not a half hour (carrying out under the condition of indoor temperature 25 DEG C); After 4 days, trollflower seed is pulled out and rinse totally, dry; Preparation liquor potassic permanganate is for subsequent use, is specially and dissolves 5 grams of potassium permanganate in 2 premium on currency; Step is dried trollflower seed liquor potassic permanganate and soak 4 hours, pull out and dry.
embodiment 2:
By the trollflower screen for seeds decontamination ghost of gathering then, eluriate with 30 DEG C of warm water, dry, for subsequent use.
Loaded by trollflower seed in cotton pocket, sealing, put into refrigerator (controlling refrigerator temperature for-2 DEG C) and preserve, take out to spring in the coming year, room temperature is placed 1 day; Get 1000 grams of pending trollflower seeds, and get 2 premium on currency and pour in bucket for subsequent use, regulate water temperature to 23 DEG C; Preparation gibberellin sodium carbonate liquor is for subsequent use, is specially to get 100 grams of sodium carbonate and 20 grams of gibberellin are dissolved in 1 premium on currency.In the water that the gibberellin sodium carbonate liquor modulated is poured into, mix well; Then trollflower seed is poured in the warm water containing gibberellin sodium carbonate liquor and be stirred to not a half hour (carrying out under the condition of indoor temperature 20 DEG C); After 4 days, trollflower seed is pulled out and rinse totally, dry; Preparation liquor potassic permanganate is for subsequent use, is specially and dissolves 5 grams of potassium permanganate in 2 premium on currency; Step is dried trollflower seed liquor potassic permanganate and soak 4 hours, pull out and dry.
Embodiment 3:
By the trollflower screen for seeds decontamination ghost of gathering then, eluriate with 40 DEG C of warm water, dry, for subsequent use.
Loaded by trollflower seed in cotton pocket, sealing, put into refrigerator (controlling refrigerator temperature for-5 DEG C) and preserve, take out to spring in the coming year, room temperature is placed 1 day; Get 1000 grams of pending trollflower seeds, and get 2 premium on currency and pour in bucket for subsequent use, regulate water temperature to 25 DEG C; Preparation gibberellin sodium carbonate liquor is for subsequent use, is specially to get 100 grams of sodium carbonate and 20 grams of gibberellin are dissolved in 1 premium on currency.In the water that the gibberellin sodium carbonate liquor modulated is poured into, mix well; Then trollflower seed is poured in the warm water containing gibberellin sodium carbonate liquor and be stirred to not a half hour (carrying out under the condition of indoor temperature 22 DEG C); After 4 days, trollflower seed is pulled out and rinse totally, dry; Preparation liquor potassic permanganate is for subsequent use, is specially and dissolves 5 grams of potassium permanganate in 2 premium on currency; Step is dried trollflower seed liquor potassic permanganate and soak 4 hours, pull out and dry.
Validity check method following (the germination test process in culture dish):
1. in culture dish, place gauze, and water and drench, arranging indoor temperature is 25 DEG C;
2. count 300 (each embodiment 100) trollflower seeds, point three sections of even spreadings wherein;
3. again gauze is covered on it, drench suitable quantity of water, make it moistening;
4. carefully look after culture dish, drench into water purification in good time, make it remain moistening humidity, and keep indoor temperature to be 20 DEG C-25 DEG C;
5., through about 15 days, budlet pips (three sections are respectively 96,95 and 95), and germination rate all reaches more than 95%.
The above is only in order to conveniently to illustrate the present invention, and in the scope not departing from the present invention's creation, the various simple covert and modification that the those skilled in the art being familiar with this technology does should belong to protection scope of the present invention.
Claims (7)
1. break the method for trollflower seed dormancy phase, it is characterized in that, comprise the steps:
1) by the trollflower screen for seeds decontamination ghost of gathering then, warm water is eluriated, and dries, for subsequent use;
2) load in cotton pocket, sealing, puts into refrigerator Cord blood, and take out to spring in the coming year, room temperature places 1 day;
3) get 1000 grams of pending trollflower seeds, and get 2 premium on currency and pour in bucket for subsequent use, regulate water temperature to 20 DEG C-25 DEG C;
4) gibberellin sodium carbonate liquor is prepared for subsequent use;
5) the gibberellin sodium carbonate liquor modulated is poured in the water of step 3), mix well;
6) then by step 2) the trollflower seed of gained stirs in pouring containing gibberellin sodium carbonate liquor warm water into;
7) after 4 days, trollflower seed is pulled out and rinse clean, dry;
8) liquor potassic permanganate is prepared for subsequent use;
9) liquor potassic permanganate of the trollflower seed step 8) gained of step 7) gained is soaked 4 hours, pull out and dry.
2. the method breaking the trollflower seed dormancy phase according to claim 1, is characterized in that: in described step 1), the temperature of warm water is 30-40 DEG C.
3. the method breaking the trollflower seed dormancy phase according to claim 1, is characterized in that: described step 2) in temperature in refrigerator be-2 DEG C--5 DEG C.
4. the method breaking the trollflower seed dormancy phase according to claim 1, is characterized in that, in described step 4), the compound method of gibberellin sodium carbonate liquor is, gets 100 grams of sodium carbonate and 20 grams of gibberellin are dissolved in 1 premium on currency.
5. the method breaking the trollflower seed dormancy phase according to claim 1, is characterized in that, in described step 6), the time of stirring is no less than half an hour.
6. the method breaking the trollflower seed dormancy phase according to claim 1, is characterized in that, keeps indoor temperature 20 DEG C-25 DEG C in described step 6).
7. the method breaking the trollflower seed dormancy phase according to claim 1, is characterized in that: in described step 8, then the preparation method of liquor potassic permanganate for dissolving 5 grams of potassium permanganate in 2 premium on currency.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510063373.8A CN104641762B (en) | 2015-02-06 | 2015-02-06 | A kind of method breaking the Flos Trollii seed dormancy phase |
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| CN201510063373.8A CN104641762B (en) | 2015-02-06 | 2015-02-06 | A kind of method breaking the Flos Trollii seed dormancy phase |
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| CN104641762A true CN104641762A (en) | 2015-05-27 |
| CN104641762B CN104641762B (en) | 2016-06-15 |
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| CN201510063373.8A Active CN104641762B (en) | 2015-02-06 | 2015-02-06 | A kind of method breaking the Flos Trollii seed dormancy phase |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106342519A (en) * | 2016-08-25 | 2017-01-25 | 宁夏明德中药饮片有限公司 | Trollius chinensis planting method |
| CN109005735A (en) * | 2018-07-25 | 2018-12-18 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | A kind of trollflower container seedling culture method |
| CN111615882A (en) * | 2020-06-12 | 2020-09-04 | 内蒙古自治区农牧业科学院 | Method for breaking dormancy of trollius chinensis seeds |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010150845A1 (en) * | 2009-06-25 | 2010-12-29 | 森下仁丹株式会社 | Method for storage of seeds |
| CN102283122A (en) * | 2011-07-11 | 2011-12-21 | 镇江瑞繁农艺有限公司 | Dormancy breaking seeding raising method for water lily seeds |
-
2015
- 2015-02-06 CN CN201510063373.8A patent/CN104641762B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010150845A1 (en) * | 2009-06-25 | 2010-12-29 | 森下仁丹株式会社 | Method for storage of seeds |
| CN102283122A (en) * | 2011-07-11 | 2011-12-21 | 镇江瑞繁农艺有限公司 | Dormancy breaking seeding raising method for water lily seeds |
Non-Patent Citations (2)
| Title |
|---|
| 丁万隆等: ""贮藏方法对打破金莲花种子休眠的影响"", 《中国中药杂志》, vol. 25, no. 5, 31 May 2000 (2000-05-31), pages 266 - 269 * |
| 孙鹏等: ""高锰酸钾及植物激素对君迁子种子萌发的影响"", 《东北林业大学学报》, vol. 42, no. 6, 30 June 2014 (2014-06-30) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106342519A (en) * | 2016-08-25 | 2017-01-25 | 宁夏明德中药饮片有限公司 | Trollius chinensis planting method |
| CN109005735A (en) * | 2018-07-25 | 2018-12-18 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | A kind of trollflower container seedling culture method |
| CN111615882A (en) * | 2020-06-12 | 2020-09-04 | 内蒙古自治区农牧业科学院 | Method for breaking dormancy of trollius chinensis seeds |
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| CN104641762B (en) | 2016-06-15 |
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