CN104630182A - Compound enzyme for growing pigs and preparation method thereof - Google Patents

Abstract

The invention discloses a compound enzyme for growing pigs and a preparation method thereof, belonging to the field of enzyme preparations. The compound enzyme for growing pigs comprises the following raw materials in parts by weight: 3-8 parts of xylanase, 8-15 parts of cellulase, 5-10 parts of beta-glucanase, 3-5 parts of mesophilic alpha-amylase, 1-2 parts of galactosidase, 1-2 parts of pectinase, 3-6 parts of lactobacillus plantarum capsules, 6-10 parts of plant preparation, 2-4 parts of ginger extract and 1-2 parts of liquorice root. The compound enzyme for growing pigs has the beneficial effects that the plant preparation, the ginger extract and liquorice root in the product are scientifically compounded, so that resurgence of the enzyme preparation is effectively slowed down; the frost and heat resistance of the compound enzyme can be enhanced, and the same enzyme activities can be maintained; a protective agent, the enzyme preparation, the plant preparation and the ginger extract jointly have synergistic effects, so that the activities and effects of the compound enzyme are furthest exerted, the use rates of feeds and the growth rates of animals are increased, the appetite and disease resistance of animals are enhanced, the shelf life of the product is lengthened, and the environment is protected.

Description

A kind of growing swine compound enzyme and preparation method thereof

Technical field

The invention belongs to enzymic preparation field, be specifically related to a kind of growing swine compound enzyme and preparation method thereof.

Background technology

The zymin applied in fodder industry at present mainly contains 4 large classes: be used for degraded cellulose, protein, starch and phytic acid respectively.

Name is called a kind of store pig complex enzyme preparation for feeding, application number: 201110026562.X, a kind of store pig complex enzyme preparation for feeding of this disclosure of the invention, comprises following eight kinds of enzymes: aspartic protease, amylase, 'beta '-mannase, zytase, beta-glucanase, cellulase, polygalacturonase and phytase; The enzyme activity ratio of described eight kinds of enzymes is followed successively by 1: (0.8-14.5): (0.19-0.3): (33.7-35): (25-30): (3.2-3.5): (0.19-2.0): (0-0.025).This invention compound enzymic preparation can be degraded various antinutritional factor in feed, reduces enteron aisle chyme viscosity, improves feedstuff metabolisable energy power; Improve the digestibility of store pig to protein and starch; Reduce the nutrient content entered in rear intestinal chyme, effectively suppress harmful microbe breeding in enteron aisle, reconcile intestinal microecology balance, promote that pig is healthy; Improve growth, fattening pig average daily gain, reduce feedstuff-meat ratio, shorten the livestock on hand phase, increase economic efficiency.

A kind of complex enzyme preparation for feeding piglets, application number is 201110026602.0, a kind of complex enzyme preparation for feeding piglets of this disclosure of the invention.This complex enzyme preparation for feeding piglets comprises following seven kinds of enzymes: aspartic protease, amylase, zytase, beta-glucanase, cellulase, polygalacturonase and phytase; The enzyme activity ratio of described seven kinds of enzymes is followed successively by 1: 1: (8-10): (3.3-4): 1: (0.5-0.55): (0-0.02).Various antinutritional factor in this invention compound enzymic preparation degradable feed, reduces enteron aisle chyme viscosity, improves feedstuff metabolisable energy power; Improve the digestibility of piglet to protein and starch; Reduce harmful microbe excessive multiplication in enteron aisle, reduce the damage of intestines wall, reconcile intestinal microecology balance; Improve piglet survival rate, disease resistance, reduce diarrhea rate, improve overall uniformity coefficient; Promote weight gain of piglets, reduce aquaculture cost.

A kind of pig's feed compound enzymic preparation being added with activator, application number: 201210500011.7, the invention provides a kind of pig's feed compound enzymic preparation being added with activator, organophosphorus in feed can be resolved into the inorganic phosphate that can absorb by the phytase in formula, protein-based nutritive substance in proteolytic enzyme degradable feed, promote that live pig is to the absorption of Dietary phosphorus and nitrogen, also reduces the quantity discharged of phosphorus and nitrogen in live pig fecaluria simultaneously, reduces its pollution on the environment.And activator can improve the speed of reaction of enzyme, zymin is taken effect faster, and each ingredient combination in activator and zymin use the shearing force of cold cuts also can be made to reduce, and improves pork tenderness.The pig's feed compound enzymic preparation that the present invention obtains can improve the digestibility of pig to nitrogen, phosphorus in feed, thus makes the excretion of phosphorus in live pig fecaluria reduce more than 40%, and the excretion of nitrogen reduces more than 29%; Instant effect, namely has significant effect in 2-3 days; Cold cuts shearing force reduces by more than 50%.

Name is called a kind of pig's feed compound enzymic preparation and preparation method thereof application number: 201410275557.6, the invention provides a kind of pig's feed compound enzymic preparation, belongs to feed additive field.This additive is made up of prozyme and toughener; Prozyme is lipase, neutral protease, beta-amylase, isoamylase, neutral phytase, 'beta '-mannase, zytase, alpha-galactosidase; Toughener is made up of extract from pine needles, leaf of bamboo Crude polysaccharides, Japanese Honeysuckle Stem powder, Rhizoma Alpiniae Officinarum powder, Radix Vladimiriae powder, Asian puccoon powder, vitamins C, sodium-chlor, Sodium propanecarboxylate, ferrous sulfate and calglucon.This zymin effectively can improve the utilization ratio of feed, reduces feedstuff-meat ratio, promotes the speed of growth of pig; Can also play the effect of the immunizing power strengthening pig, to the health degree improving pig, reduce pig sickness rate and have very large help, market outlook are very wide.

Name is called a kind of efficient pig's feed compound enzymic preparation being added with enzymatic protective reagent, application number is 201210381755.1, the invention provides a kind of efficient pig's feed compound enzymic preparation being added with enzymatic protective reagent, the materials such as antinutritional factor and biomacromolecule such as the beta-glucan in degradable feed, phytic acid, pectin, thus promote that pig is to the absorption of feed Middle nutrition composition, improves efficiency of feed utilization; Protectantly add the inactivation rate that can reduce enzyme, extend working lipe, make the effect of this zymin more significantly and lasting; The high efficiency composition zymin feed conversion rate that the present invention obtains is high, and feedstuff-meat ratio can be made to decline more than 19%; The pig speed of growth improves more than 23%; The enzymic activity hold-time is long, and in 18 months, enzymic activity can remain on more than 90%.

Name is called a kind of efficient pig's feed compound enzymic preparation being added with enzymatic protective reagent, application number: 201210360110.X, the invention provides a kind of efficient pig's feed compound enzymic preparation being added with enzymatic protective reagent, the materials such as antinutritional factor and biomacromolecule such as the beta-glucan in degradable feed, phytic acid, pectin, thus promote that pig is to the absorption of feed Middle nutrition composition, improves efficiency of feed utilization; Protectantly add the inactivation rate that can reduce enzyme, extend working lipe, make the effect of this zymin more significantly and lasting; The high efficiency composition zymin feed conversion rate that the present invention obtains is high, and feed coefficient can be used to decline more than 19%; The pig speed of growth improves more than 23%; The enzymic activity hold-time is long, and in 30 months, enzymic activity can remain on more than 90%.

Name is called a kind of the feed for piglet compound enzymic preparation and production method thereof and application, application number: 201410150709.X, a kind of the feed for piglet compound enzymic preparation of this disclosure of the invention and production method thereof and application.The enzyme of this compound enzymic preparation is lived and is consisted of: glucose oxidase 80-100U/g, alpha-galactosidase 20-25U/g, zytase 3000-4000U/g, beta-glucanase 500-600U/g, 'beta '-mannase 500-600U/g, aspartic protease 2000-3000U/g, neutral protease 800-1000U/g, mesophilicα-diastase 800-1000U/g, fungal alpha-amylase 400-500U/g, glucoamylase 3000-4000U/g.This compound enzymic preparation adopts W-Gum and potassium primary phosphate, dipotassium hydrogen phosphate, mixture of calcium sulfate as thinner.This compound enzymic preparation using method is: the addition in suckling piglet perfect compound feed per ton is 500-750 gram.

The bark of eucommia, is rich in phenylpropanoids, iridoids isoreactivity composition, has rubbish in purged body, strengthens cellular material metabolism, decomposer inner cholesterol, reduces body fat, broad-spectrum antimicrobial, improves white blood cell count, the remarkable efficacy such as strengthening immunity.The result of pure eucommia bark powder in experimentation on animals shows: can promote cholesterol and lipid metabolism; Add the eel that pure eucommia bark powder is raised, there is the taste of natural eel; Pure eucommia bark powder is added to the quality that can improve broiler chicken in feed, make it that there is tempting fragrance and quality.

Poria cocos has promoting diuresis to eliminate damp pathogen, invigorating the spleen for dissipating phlegm, tranquillizing by calming the heart, and relieve internal heat anticancer effect, and can relax gastrointestinal smooth muscle, the effects such as gastric acid secretion inhibiting, prevents hepatic necrosis, antibacterial; Contained pachymic acid has strengthening immunity, antitumor and calm, hypoglycemic etc. effect; Pachymose can reduce malaber reefcod serum A/G value, improves serum complement C3 content, improves blood leucocyte number and leukocytes phagocytic rate, improves its non-specific immunity.

In sum, the market space that the application of growing swine specific enzyme has it wide and huge economic worth, but the thermostability of growing swine specific enzyme, security, composite comprehensive and action effect give full play to the major issue being still zymin manufacturer and numerous raisers and jointly paying close attention to, prepare safer, more comprehensively, the better growing swine specific enzyme of enzyme action effect is corporation responsibility and the pursuit of industry technician.

Summary of the invention

Technical problem solved by the invention provides a kind of growing swine compound enzyme;

Described growing swine compound enzyme, be made up of the raw material of following parts by weight: zytase 3-8, cellulase 8-15, beta-glucanase 5-10, mesophilicα-diastase 3-5, tilactase 1-2, polygalacturonase 1-2, plant lactobacillus capsule 3-6, galenical 6-10 part, Rhizoma Zingiberis Recens extract 2-4 part, Radix Glycyrrhizae 1-2 part.

Described zytase, beta-glucanase, cellulase, mesophilicα-diastase, tilactase are food-grade enzyme preparation;

The preparation method of described galenical is as follows:

Take Semen Cassiae 10-18 part; Bark of eucommia 5-15 part; Poria cocos 10-15 part; Respectively said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 3-6 times of weight in container, add the acidic cellulase of said mixture material constituent mass 1-2%, control temperature 45-55 DEG C, pH:3.5-4, keeps 1-2h, adds the mixture of mixture 2-3 times of w ethanol and propyl alcohol subsequently, control temperature to 60 DEG C-78 DEG C of maintenance 3-4h, filter; Filter vacuum concentrates postlyophilization and obtains galenical.

The mass ratio of described ethanol and propyl alcohol is 1:1-1.5; Alcohol concn is 95%; Propanol concentration is 100%;

The preparation method of described Rhizoma Zingiberis Recens extract is as follows:

To be crushed to particle diameter is that less than 2 millimeters gingers are placed in container, adds the water of ginger 3-6 times of weight, and control temperature 50 DEG C-60 DEG C keeps 2-3h, adds the mixture of ginger 2-3 times of w ethanol and methyl alcohol, and control temperature to 30 DEG C-40 DEG C of maintenance 3-8h, filter; Filter vacuum concentrates postlyophilization and obtains Rhizoma Zingiberis Recens extract.

The mass ratio of described ethanol and methyl alcohol is 1:2, alcohol concn 85-95%; Methanol concentration 100%.

The preparation method of described plant lactobacillus capsule is as follows:

Plant lactobacillus capsule product is made up of wall material, plant lactobacillus, lyophilized vaccine, stachyose and morel enzymolysis powder.

Described wall material is made up of enzymatic soybean protein isolate, chitosan, xanthan gum, carrageenin, glycerine and trehalose; Concentration when above-mentioned each material is made into mixing solutions is respectively: enzymatic soybean protein isolate 7-10%, chitosan 1-2%, xanthan gum 1-3%, carrageenin 0.1-0.5%, glycerine 0.3-1%, trehalose 0.5-2%.

The preparation method of enzymatic soybean protein isolate is: compound concentration is that 10-13% soybean protein isolate solution is heated to 30-45 DEG C, adjustment pH to 3-5, add the aspartic protease insulation enzymolysis 0.5-1.5 hour of soybean protein isolate weight 0.1-1%, after enzymolysis, solution spray drying obtains enzymatic soybean protein isolate.

Morel enzymolysis powder, preparation method thereof is as follows:

(1) Morchella esculenta (L.) Pers sporophore drying and crushing;

(2) water-soluble homogeneous: the Morchella esculenta (L.) Pers sporophore of pulverizing is added in stainless steel cylinder, add the water of sporophore 3-6 times of weight, soak 3-5 hour, then this Morchella esculenta (L.) Pers sporophore liquid is passed through colloidal mill, colloid mill operation condition is: adjustment colloidal mill stator and the gap of rotor are 0.5-1 micron, colloidal mill flow be 0.4-1 ton/hour;

(3) intensification enzymolysis: the Morchella esculenta (L.) Pers sporophore liquid mixed solution through milling treatment of colloid is transferred in stainless steel enzymatic vessel and is heated to 50-60 DEG C, adjustment pH to 4.5-6.0, add the cellulase of Morchella esculenta (L.) Pers sporophore weight 0.05-0.1%, the beta-glucanase of 0.01-0.1%, the proteolytic enzyme of 0.01-0.1%, insulation enzymolysis 0.5-1.5 hour, constantly stirs in enzymolysis process.

(4) dry: dry acquisition morel enzymolysis powder after the mash filtrations after enzymolysis.

Described drying can adopt the dried forms such as conventional spraying dry, lyophilize.

Described lyophilized vaccine is made up of skim-milk, trehalose and maltodextrin.

The preferred CGMCC NO.9405 of the plant lactobacillus that capsule product of the present invention comprises.

Above-mentioned plant lactobacillus capsule product preparation method is as follows:

1) core is prepared: in the plant lactobacillus fermented liquid cultivated through ferment tank, add the stachyose of fermented liquid quality 3-5% and the morel enzymolysis powder of 5-8%, then mix with frozen-dried protective agent solution, pre-freeze 0.5h at-50 DEG C, then freeze-drying 10-18h in vacuum freeze drier, grinds after freeze-drying and makes core;

The ratio of described fermented liquid and frozen-dried protective agent solution is 1:1.4-0.8; Described frozen-dried protective agent solution is made up of skim-milk, trehalose and maltodextrin solution; The volume ratio of three kinds of solution is skim-milk: trehalose: maltodextrin=3:1:0.5;

The concentration of described skim-milk, trehalose and maltodextrin solution is respectively: skim-milk 5%, trehalose 1%, maltodextrin 2%.

2) bag quilt: core is suspended in fluidized-bed, wall material spraying bag quilt, the mixed solution of chitosan, glycerine and trehalose is sprayed into from a shower nozzle, the mixed solution of xanthan gum, carrageenin and enzymatic soybean protein isolate is sprayed into from another shower nozzle, spray velocity controls identical, bag between 25-38 DEG C, is namely formed capsule after 30 minutes by temperature in fluidized-bed in process.This drying means adopts device processes in patent 201120503311.1.

Described mesophilicα-diastase has following characteristic:

(1) this enzyme thermal adaptation a wider range, temperature activity between 55-75 DEG C is higher, and optimum temperature is 65 DEG C;

Thermostability: this enzyme is preserved 24h and still had the work of 80-83% enzyme under 65 DEG C of conditions, preserves 12h and still has the work of more than 50-60% enzyme under 70 DEG C of conditions.

(2) this enzyme optimal reaction pH value is 5.0; High enzyme vigor is all had between pH value 4.0-6.0;

Acid acceptance: still have the enzyme of more than 80% to live preserve 18h under pH4-6 after.

(3) enzymic activity: bacterial strain 304 is 7000-9600U/ml by the acid mesophilicα-diastase enzyme activity that fermentation is standby, is particularly suitable for liquefaction process and Mashing process and the industrialization demand of depositing.

Described amylase is mesophilicα-diastase, adopts solution fermentation to cultivate and obtains.

The bacterial strain of product mesophilicα-diastase provided by the invention is specially subtilis 304 (Bacillus subtilis) 304.This bacterial strain on November 24th, 2013 be preserved in China typical culture collection center (be called for short CCTCC, address is: China. Wuhan. Wuhan University), preserving number is CCTCC NO:M 2013600.

Described subtilis 304 is separated by a strain and obtains through UV-LiCl-ethyl sulfate Mutation screening from the subtilis starting strain of the product mesophilicα-diastase of acid soil, bacterial strain feature is as follows: described bacterial strain colony colour on solid plate is oyster white, surface drying fold, look secretly opaque, neat in edge, microscopy is elongated rod shape, and gramstaining is positive, peritrichous, gemma ovalize.

The preparation method of growing swine compound enzyme of the present invention is as follows:

By described Radix Glycyrrhizae, galenical and Rhizoma Zingiberis Recens extract micronizing respectively, then with zytase, cellulase, beta-glucanase, amylase, tilactase, polygalacturonase, packs the growing swine compound enzyme that gets product after plant lactobacillus capsule Homogeneous phase mixing.

Described enzyme is applicable to feed-processing plant and plant's autogamy feed, should mix, can the present invention be mixed with a small amount of feed in advance during use with other raw material in feed, remix in large quantities of feed, Direct-fed; Advise that complete diet pellet addition per ton is: 100-150g.

Beneficial effect:

The present invention adopts the zymotechnique of gradient cooling and gradient increased temperature with mesophilicα-diastase, and added inoculation and in good time feed supplement, the present invention is produced, and mesophilicα-diastase vigor is high, tolerable temperature is high, stability is strong, be suitable for suitability for industrialized production simultaneously.Enzyme activity is 9500U/ml.Measure enzyme work after middle temperature Alpha-starch crude enzyme liquid sample preserves the different time at 40-90 DEG C, result shows, and sample is preserved 24h and still had 83% enzyme work under 65 DEG C of conditions, preserves 12h and still have more than 60% enzyme work under 70 DEG C of conditions.

Adopt galenical, Rhizoma Zingiberis Recens extract, Radix Glycyrrhizae science composite in product of the present invention, effectively slow down the moisture regain of zymin; Can strengthen prozyme simultaneously resistance toly to freeze, resistance toheat, keep identical enzyme activity, its heat resisting temperature can improve 10-15 DEG C, resistance to freezing temp can reduce 5-10 DEG C, effectively prevent the loss of prozyme enzyme activity in transport, preservation and use procedure, extend the quality guaranteed period of prozyme, reach same enzyme activity, the like product quality guaranteed period can extend 3-5 month.

The galenical that product of the present invention adds both can extend the quality guaranteed period of compound enzymic preparation, can improve again the immunizing power of raising livestock and poultry, effectively prevent the generation of livestock and poultry epidemic disease.

Lactobacillus micro-capsule provided by the invention, modified soybean protein isolate is with the addition of in its wall material, modified soybean protein isolate improves 15-25% than soybean protein isolate emulsifying capacity raising 25%, gelling ability, modified soybean protein isolate xanthan gum and carrageenin and chitosan with the use of, gel-strength improves more than 10%, enhances the stomach juice-resistant of capsule; Capsule of the present invention adopts modified soybean protein isolate, has good enteric solubility, complete disintegration can discharge plant lactobacillus, and propagation becomes dominant microflora rapidly after capsule arrives enteron aisle in 1-1.5 hour, thus reaches effects such as suppressing growth of pathogenic bacteria.

The common synergy of protective material and zymin, galenical, Rhizoma Zingiberis Recens extract and zymin in product of the present invention; the enzyme activity of prozyme and effect are played to greatest extent; and improve the utilization ratio of feed and the growth rate of animal accordingly; enhance appetite and the resistance against diseases of animal, extend the quality guaranteed period of product and protect environment.

Embodiment

The present invention is described below by specific embodiment.Unless stated otherwise, technique means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.

Plant lactobacillus provided by the present invention (Lactobacillus plantarum) tlj-2014, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.9405, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101, preservation date on July 2nd, 2014.

Embodiment 1

The Breeding Process of the present invention's plant lactobacillus used CGMCC No.9405 is as follows:

The original bacterial classification that sets out → test tube activation → ethyl sulfate (DES) mutagenesis → nitrosoguanidine (NTG) mutagenesis → plasma body mutagenesis → dull and stereotyped primary dcreening operation → shaking flask sieves → mitotic stability test again.

Starting strain of the present invention is in MRS dextrose culture-medium, and the throughput rate of its lactic acid is 1.5g/L/d, almost stops growing when medium pH is 3.5, is 0.34mg/h/kg Chinese cabbage to the rate of decomposition of Sodium Nitrite.Starting strain is collected in the greenfeed of Fattening Sheep field, Yanchi county Ningxia by Li Zheng, acquisition time on September 15th, 2013.

In order to improve the rate of decomposition of its lactic acid-producing speed, acid-fast ability and nitrite, DES and NTG technology is adopted to carry out mutagenesis to this bacterial classification successively, after mutagenesis, bacterial strain adopts MRS calcium carbonate flat board to carry out primary dcreening operation, then 500mL shake flask fermentation is adopted, biosensor analysis instrument carries out multiple sieve to Producing Strain, the lactobacterium plantarum strain that seed selection is excellent, then does passage assays, evaluates its genetic stability.

Plant lactobacillus tlj-2014 genetic stability result shows: through continuous passage ten times, property indices is all more stable, and heredity is better, and proterties is not replied, therefore using the object bacterial strain that plant lactobacillus tlj-2014 obtains as seed selection.

Empirical tests finds: the lactic acid-producing speed of this mutagenic strain can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L; Can survive under pH is the condition of 1.80.Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of spontaneous fermentation process nitrite accumulation is approximately 1.1mg/h/kg), can resistance to 1% cholate.

1) DES mutagenic and breeding

A. on super clean bench, get plant lactobacillus L mono-ring (set out bacterium) on test tube slant, access is equipped with in the 250mL triangular flask of 50mL substratum MRS (without agar, glucose 20g/L), 200rpm, cultivate about 12h for 37 DEG C, make thalline be in logarithmic growth in earlier stage.

B. get 5mL bacterium liquid, the centrifugal 10min of 5000rpm collects thalline, with brine 2 times.

C. 107/mL bacteria suspension is diluted to pH7.0 phosphoric acid buffer.

D. get the potassium phosphate buffer of 32mL pH7.0,8mL bacteria suspension, 150mL triangular flask that 0.4mL DES to put into rotor in advance fully mix, make DES ultimate density be 1% (v/v).

E. in 37 DEG C of shaking tables, 150rpm reacts 30min, gets 1mL mixed solution, adds 0.5mL 25%Na2S2O3 solution stopped reaction.

F. suitably dilute, get last dilution bacterium liquid 0.2mL, coat in calcium carbonate screening culture medium (the calcium carbonate MRS substratum containing 100g/L glucose) plate.Cultivate after 2 ~ 3 days at 37 DEG C, adopting photolithography the bacterial strain of this screening flat board to be transferred to pH is in the upper and Sodium Nitrite screening culture medium (single nitrogenous source is the modification MRS screening culture medium of 2g/L Sodium Nitrite) of LPHMRS substratum (low ph value modification MRS substratum) of 1.5,1.8 and 2.0.

G. cultivate after 2 ~ 3 days at 37 DEG C, choosing colony is comparatively large, can grow respectively and in LPHMRS substratum, Sodium Nitrite screening culture medium in calcium carbonate screening culture medium.Through preliminary screening, the bacterium colony called after plant lactobacillus L1 that picking goes out.

2) nitrosoguanidine mutagenesis

A. on super clean bench, get plant lactobacillus L1 mono-ring on test tube slant, access is equipped with in the 250mL triangular flask of 50mL substratum MRS (without agar) (glucose concn is 60g/L), 200rpm, cultivates about 12h for 37 DEG C, makes thalline be in logarithmic growth in earlier stage.

B. get the centrifugal 10min of 5mL bacterium liquid 5000rpm and collect thalline, with brine 2 times.

C. 107/mL bacteria suspension is diluted to pH6.0 phosphoric acid buffer.

D. get 10mL bacteria suspension to be transferred in 100mL triangular flask, add the NTG of 10mg, be mixed with the NTG solution that final concentration is 10mg/mL, and add 4-5 and drip acetone, be beneficial to NTG and dissolve.

E. at 37 DEG C, the centrifugal 10min of 200rpm oscillatory reaction 30min, 5000rpm collects thalline, with stroke-physiological saline solution washing several, and stopped reaction.

F. suitably dilute, get last dilution bacterium liquid 0.2mL, coat in calcium carbonate screening culture medium (the calcium carbonate MRS substratum containing 100g/L glucose) plate.Cultivate after 2 ~ 3 days at 37 DEG C, adopting photolithography the bacterial strain of this screening flat board to be transferred to pH is in the upper and Sodium Nitrite screening culture medium (single nitrogenous source is the modification MRS screening culture medium of 2g/L Sodium Nitrite) of LPHMRS substratum (low ph value modification MRS substratum) of 1.5,1.8 and 2.0.

G. select bacterial strain method: choosing colony is comparatively large, to grow in LPHMRS substratum, Sodium Nitrite screening culture medium respectively and in calcium carbonate screening culture medium.Through preliminary screening, picking 100 meets the bacterium colony of above condition.

3) shaking flask is sieved again

A. on super clean bench, get plant lactobacillus one ring on each test tube slant respectively, access is equipped with in the 250mL triangular flask of 50mL substratum MRS (without agar) (glucose concn is 100g/L), 200rpm, cultivates about 15h, makes thalline be in mid log phase for 37 DEG C.

B. get 5mL bacterium liquid respectively, LPHMRS liquid nutrient medium (low ph value modification MRS substratum) and the Sodium Nitrite liquid screening medium (single nitrogenous source is the modification MRS screening culture medium of 2g/L Sodium Nitrite) upper (note: adopt 250mL triangular flask) that 50mL calcium carbonate screens in liquid nutrient medium (the calcium carbonate MRS substratum containing 250g/L glucose) plate, pH is 1.5,1.8 and 2.0 is equipped with in access.200rpm, cultivates 3-4 days for 37 DEG C, detects the wear rate that Pfansteihl in calcium carbonate screening liquid nutrient medium produces speed, biomass in LPHMRS liquid nutrient medium and Sodium Nitrite liquid screening medium nitrite every day respectively.After fermentation ends, compare the wear rate that Pfansteihl in the calcium carbonate screening liquid nutrient medium of 100 strain bacterial classifications produces speed, biomass in LPHMRS liquid nutrient medium and Sodium Nitrite liquid screening medium nitrite.

C. select to have concurrently the bacterial strain that high Pfansteihl produces speed, the wear rate of tolerate low pH (this bacterial classification only can grow in the minimum substratum for pH1.8) and nitrite is high, by its called after L2 bacterium.

4) genetic stability test

L2 bacterium is gone down to posterity for continuous ten times on inclined-plane, and detects the fermentation situation after at every turn going down to posterity by the method that shaking flask is sieved again.Experiment finds, inclined-plane goes down to posterity for continuous ten times, and this bacterial classification proterties does not have considerable change, and property indices is all normal, illustrates that the genetic stability of this bacterial classification is stronger.Strain Designation is plant lactobacillus (Lactobacillus plantarum) tlj-2014.

5) 5L fermentor tank test

A. get plant lactobacillus L2 mono-ring on inclined-plane, access is equipped with in the 250mL triangular flask of 50mL substratum MRS (without agar) (glucose concn is 150g/L), 200rpm, cultivates about 12h, makes thalline be in mid log phase for 37 DEG C.

B. the access of the bacterial classification of logarithmic phase is equipped with in the 5L fermentor tank of 3L MRS liquid nutrient medium (initial glucose is 150g/L).Inoculum size is that at 10%, 37 DEG C, 100rpm cultivates 8 hours, and logarithm dissolved oxygen in early stage controls 10% (ventilation 0.5L/min), later stage Anaerobic culturel 63 hours.After fermentation ends, the lactic acid production of plant lactobacillus L2 reaches 95g/L.

C. 3L pH being equipped with in the access of the bacterial classification of logarithmic phase is in the 5L fermentor tank of LPHMRS liquid nutrient medium (initial glucose is 50g/L) of 1.8.Inoculum size is that at 10%, 37 DEG C, 100rpm cultivates 8 hours, and logarithm dissolved oxygen in early stage controls 10% (ventilation 0.5L/min), and later stage anaerobism, fermented liquid pH controls 1.8 by the sodium hydroxide of whole process 0.5mol/L, and total incubation time is 48 hours.After fermentation ends, the biomass detecting plant lactobacillus L2 is 2.5g/L, illustrates that plant lactobacillus L2 can survive in the environment of pH1.8.

D. the access of the bacterial classification of logarithmic phase is equipped with in the 5L fermentor tank of 3L Sodium Nitrite liquid screening medium (single nitrogenous source is the modification MRS screening culture medium of 2g/L Sodium Nitrite).Inoculum size is that at 10%, 37 DEG C, 100rpm cultivates 8 hours, and logarithm dissolved oxygen in early stage controls 10% (ventilation 0.5L/min), and later stage anaerobism, fermenting process adds the sodium nitrite solution of 20g/L according to the wear rate stream of nitrite, cultivates 2-3 days.After fermentation ends, calculate fermenting process plant lactobacillus L2 to the degradation rate of Sodium Nitrite.Found that: under this condition, L2 can reach 563mg/h/L to the degradation rate of Sodium Nitrite.

Embodiment 2

A preparation method for mesophilicα-diastase, comprises the steps:

(1) actication of culture

The slant strains of intact subtilis 304 is inoculated in slant medium, cultivates 24h for 31 DEG C and carry out actication of culture, so activation 2 times;

Described slant medium consists of: extractum carnis 3g, sodium-chlor 5g, peptone 10g, glucose 2g, agar 15g, distilled water l000mL, pH value 6,121 DEG C of sterilizing 20min;

(2) liquid seeds enlarged culturing

1. first order seed is cultivated: slant strains 2 articulating after step (1) being activated enters in 500 ml shake flasks, substratum loading amount 100 milliliters, rotary shaker 250rpm, culture temperature 31 DEG C, incubation time 10h;

2. secondary seed cultivate: by first order seed according to 10% inoculum size access in 500 milliliters of secondary seed shaking flasks, culture condition is identical with first order seed;

3. three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum size, substratum loading amount 1000 milliliters, rotary shaker 250rpm, culture temperature 38 DEG C, incubation time 10h;

4. first class seed pot is cultivated: the first class seed pot being 150L with 10% inoculum size access cubic capacity by three grades of seeds, fermention medium loading amount 100L, culture temperature 31 DEG C, stirring velocity 200rpm, ventilation (V/V) 1:1, tank pressure 0.05Mpa, incubation time 10h;

Institute's one-level, secondary, three grades of seed culture medium weight consist of:

Yeast powder 0.3%, glucose 1%, peptone 0.3%, extractum carnis 0.5%, dipotassium hydrogen phosphate 0.8%, herbal mediciment powder 1.5%, trehalose 1%, calcium sulfate 1g, magnesium chloride 2g, Trisodium Citrate 1g, insufficient section pure water is supplied, pH value 6,121 DEG C of sterilizing 30min.

Described seed tank culture basic weight amount consists of:

Maltodextrin 5%, yeast powder 0.4%, herbal mediciment powder 1.5%, trehalose 1%, peptone 0.1%, corn steep liquor 0.1%, dipotassium hydrogen phosphate 0.8%, magnesium sulfate 0.05%, Trisodium Citrate 0.1%, insufficient section pure water is supplied, pH value 6,121 DEG C of sterilizing 30min.

Described seeding tank fermented liquid cell concentration is 7.0 x 10 ⁸individual/ml;

(3) ferment tank

By seeding tank fermented liquid with 6% inoculum size access fermentor tank, culture temperature 35 DEG C, stirring velocity 250r/min, ventilation (V/V) 1:2, incubation time 13h; Then with 1.5 DEG C/h rate of temperature fall slow cooling to 15 DEG C, constant temperature culture 13h; Continue with 1.5 DEG C/h rate of temperature fall slow cooling to 3 DEG C, now, first class seed pot fermented liquid is added access fermentor tank with 2% inoculum size, constant temperature culture 13h; Finally slowly rise to 35 DEG C, constant temperature culture 30h with 1.5 DEG C/h temperature rise rate.

PH controls: by mending ammoniacal liquor or dilute phosphoric acid, controls pH value in fermenting process and remains on 6.5;

Described fermention medium consists of: maltodextrin 100g, Semen Maydis powder 55g, soybean cake powder 20g, herbal mediciment powder 40g, trehalose 35g, yeast powder 6g, corn steep liquor 3g, ammonium sulfate 2g, dipotassium hydrogen phosphate 2g, potassium primary phosphate 2g, Trisodium Citrate 3g, defoamer 0.5g, pure water l000mL, pH value 6.5,121 DEG C of sterilizing 20min;

The preparation method of described Chinese herbal medicine powder is as follows:

Take the Radix Astragali 25 parts, Radix Codonopsis 15 parts, radix bupleuri 15 parts, the root of large-flowered skullcap 10 parts, respectively said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 5 times of weight in container, control temperature is to 33 DEG C, pH value 6.5, 0.3% cellulase degradation is added 1.5 hours by mixture mass ratio, control temperature 80 DEG C keeps 8 minutes, then 50 DEG C are cooled to, pH is 6.0, papoid and the pectinase enzymatic hydrolysis 50 minutes of 0.20% is added respectively by mixture weight ratio, 8 DEG C are cooled to keep 2 hours, increase the temperature to 90 DEG C to keep 15 minutes, finally add the mixture of mixture 2 times of w ethanol and propyl alcohol, control temperature to 70 DEG C keeps 3.4h, filter, filter vacuum concentrates postlyophilization and obtains Chinese herbal medicine powder.

The mass ratio of described ethanol and propyl alcohol is 1:1.2.

(1) fermentation liquor filter, concentrated, allotment, essence filter, dry mesophilicα-diastase.

Obtaining warm Alpha-starch crude enzyme liquid enzyme activity in fermentation liquor centrifuging and taking supernatant liquor gained through above-mentioned preparation method is 9500U/ml.Measure enzyme work after middle temperature Alpha-starch crude enzyme liquid sample preserves the different time at 40-90 DEG C, result shows, and sample is preserved 24h and still had 83% enzyme work under 65 DEG C of conditions, preserves 12h and still have more than 60% enzyme work under 70 DEG C of conditions;

Embodiment 3

Plant lactobacillus capsule product is made up of wall material, plant lactobacillus, lyophilized vaccine, stachyose and morel enzymolysis powder.

Described wall material is made up of enzymatic soybean protein isolate, chitosan, xanthan gum, carrageenin, glycerine and trehalose; Concentration when above-mentioned each material is made into mixing solutions is respectively: enzymatic soybean protein isolate 10%, chitosan 1%, xanthan gum 3%, carrageenin 0.1%, glycerine 0.5%, trehalose 0.5%.

The preparation method of enzymatic soybean protein isolate is: compound concentration is that the soybean protein isolate solution of 10-13% is heated to 30-33 DEG C, pH is to 3 in adjustment, the aspartic protease adding soybean protein isolate weight 0.2% is incubated enzymolysis 1.5 hours, and after enzymolysis, solution spray drying obtains enzymatic soybean protein isolate.

Plant lactobacillus is deposit number CGMCC NO.9405 bacterial classification.

The preparation method of described morel enzymolysis powder is as follows:

(1) Morchella esculenta (L.) Pers sporophore drying and crushing;

(2) water-soluble homogeneous: the Morchella esculenta (L.) Pers sporophore of pulverizing is added in stainless steel cylinder, add the water of sporophore 3 times of weight, soak 5 hours, then this Morchella esculenta (L.) Pers sporophore liquid is passed through colloidal mill, colloid mill operation condition is: the gap of adjustment colloidal mill stator and rotor is 0.6 ± 1 micron, and colloidal mill flow is 0.5 ton/hour;

(3) intensification enzymolysis: the Morchella esculenta (L.) Pers sporophore liquid mixed solution through milling treatment of colloid is transferred in stainless steel enzymatic vessel and is heated to 50 DEG C, pH is to 6.0 in adjustment, add the cellulase of Morchella esculenta (L.) Pers sporophore weight 0.05%, the beta-glucanase of 0.01%, the proteolytic enzyme of 0.1%, insulation enzymolysis 1.5 hours, constantly stirs in enzymolysis process.

(4) dry: after the mash filtrations after enzymolysis, spraying dry obtains morel enzymolysis powder.

The preparation process of above-mentioned plant lactobacillus capsule product is as follows:

1) core is prepared: in the plant lactobacillus fermented liquid cultivated through ferment tank, add the stachyose of fermented liquid quality 3% and the morel enzymolysis powder of 8%, then mix with frozen-dried protective agent solution, pre-freeze 0.5h at-50 DEG C, then freeze-drying 10h in vacuum freeze drier, grinds after freeze-drying and makes core; The ratio of fermented liquid and frozen-dried protective agent solution is 1:1.4; Frozen-dried protective agent solution is made up of skim-milk, trehalose and maltodextrin solution; The volume ratio of three kinds of solution is skim-milk: trehalose: maltodextrin=3:1:0.5;

The concentration of described skim-milk, trehalose and maltodextrin solution is respectively: skim-milk 5%, trehalose 1%, maltodextrin 2%.

2) bag quilt: core is suspended in fluidized-bed, wall material spraying bag quilt, the mixed solution of chitosan, glycerine and trehalose is sprayed into from a shower nozzle, the mixed solution of xanthan gum, carrageenin and enzymatic soybean protein isolate is sprayed into from another shower nozzle, spray velocity controls identical, bag at 28 DEG C, is namely formed capsule after 30 minutes by temperature in fluidized-bed in process.Adopt device preparation as described in patent 201120503311.1.

Plant lactobacillus capsule product bile tolerance is tested.

Capsule product in embodiment 2,3 be placed in pig cholate solution (strength of solution 3.0%) insulation of 37 DEG C and constantly stir, take out after 2h, with sterile saline washing, with opening one's purse, liquid dissolves, measure Lactobacillus Survival, and compare with the bacterium liquid do not embedded.Test result is as shown in the table.

	Metamorphosis	Survival rate
			Embodiment 1	Not disintegration	87.5％
Embodiment 2	Not disintegration	89.3％
			Do not wrap and contrasted	--	0.65％

Embodiment 4

A kind of growing swine compound enzyme, is made up of the raw material of following parts by weight: zytase 3, cellulase 10, beta-glucanase 8, mesophilicα-diastase 4, tilactase 1, polygalacturonase 1, plant lactobacillus capsule 5, galenical 8 parts, Rhizoma Zingiberis Recens extract 3 parts, 1.5 parts, Radix Glycyrrhizae.

The preparation method of described galenical is as follows:

Take Semen Cassiae 15 parts; The bark of eucommia 10 parts; 12 parts, Poria cocos; Respectively said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 5 times of weight in container, add the acidic cellulase of said mixture material constituent mass 2%, control temperature 50 DEG C, pH:3.8, keeps 1h, adds the mixture of mixture 3 times of w ethanol and propyl alcohol subsequently, control temperature to 70 DEG C keeps 3h, filters; Filter vacuum concentrates postlyophilization and obtains galenical.

The mass ratio of described ethanol and propyl alcohol is 1:1; Alcohol concn is 95%; Propanol concentration is 100%;

The preparation method of described Rhizoma Zingiberis Recens extract is as follows:

To be crushed to particle diameter is that less than 2 millimeters gingers are placed in container, adds the water of ginger 4 times of weight, and control temperature 55 DEG C keeps 2h, adds the mixture of ginger 3 times of w ethanol and methyl alcohol, and control temperature to 35 DEG C keeps 5h, filters; Filter vacuum concentrates postlyophilization and obtains Rhizoma Zingiberis Recens extract.

The mass ratio of described ethanol and methyl alcohol is 1:2, alcohol concn 90%; Methanol concentration 100%.

Embodiment 5

Basic with routine 1-4

A kind of growing swine compound enzyme, is made up of the raw material of following parts by weight: zytase 8, cellulase 15, beta-glucanase 5, mesophilicα-diastase 5, tilactase 2, polygalacturonase 2, plant lactobacillus capsule 3, galenical 10 parts, Rhizoma Zingiberis Recens extract 2 parts, 2 parts, Radix Glycyrrhizae.

Described zytase, beta-glucanase, cellulase, mesophilicα-diastase, tilactase are food-grade enzyme preparation;

Embodiment 6

A kind of growing swine compound enzyme, is made up of the raw material of following parts by weight: zytase 5, cellulase 8, beta-glucanase 10, mesophilicα-diastase 5, tilactase 1, polygalacturonase 1, plant lactobacillus capsule 3, galenical 6 parts, Rhizoma Zingiberis Recens extract 2 parts, 1 part, Radix Glycyrrhizae.

The preparation method of growing swine compound enzyme of the present invention is as follows:

By described Radix Glycyrrhizae, galenical and Rhizoma Zingiberis Recens extract micronizing respectively, then with zytase, cellulase, beta-glucanase, amylase, tilactase, polygalacturonase, packs the growing swine compound enzyme that gets product after plant lactobacillus capsule Homogeneous phase mixing.

Feeding experiment:

Materials and methods

The selection of test pig and grouping

The a certain large-scale regular pig farm in Hunan, chooses the healthy DLY three way cross growing swine that 36 body weight are (30 ± 2) kg, is divided into 3 groups at random, often organizes 2 hurdles and repeats, often repeat 6, galt and gilt half and half.The prerun of 1 week official test advance behavior phase, and complete expelling parasite and routine immunization work.Test point two phases of front and back carry out, in earlier stage (30kg-60kg), and the later stage (61kg ~ 90kg).

Feeding and management

Feed dry mash, free choice feeding, is limited cannot not have enough surplusly, and automatic drinking bowl is drunk water.Day in early stage feeds three times, and day in later stage feeds secondary, in units of hurdle, record feed consumption rate.Pig house is brick structure, cement flooring, single-column type, and examination pig is raised respectively in 9 hurdles, and the envrionment conditions of each column home is consistent, cleans colony house morning and afternoon every day each once, observes the behavior of pig, appetite, ight soil simultaneously.Duration of test record disease and treatment situation.

Feed and formula

Based on full price GB growing swine feed, feed per ton adds the growing swine specific enzyme 100g of test group, control group 1 and control group 2;

Test index: before the test at the end of (30kg-60kg), mid-term and later stage (61kg ~ 90kg), point another name individual weight, weighs and carries out on an empty stomach all in the morning.Stage by stage, record its every daily material consumption, sickness rate in units of hurdle, calculate daily ingestion amount and food utilization efficiency simultaneously, and carry out statistical study to above-mentioned data, growing swine growth performance is in table 2.

Table 2

Analysis is carried out as table 3 to the full phase test result of above-mentioned test:

Table 3

Project	Test group	Control group 1	Deviation (%)	Control group 2	Deviation (%)
						Day weight gain (g)	877	746	131(17.56)	657	220(33.49)
Food consumption (kg)	189.73	193.46	-3.73(-1.92)	210.17	-20.44(-9.7)
						Feed-weight ratio	2.80	3.36	-0.56(-16.67)	4.15	-1.35(-32.53)
Diarrhea rate (%)	0	4	-4(-100)	17	-17(-100)
						Hair color is marked	9	6.5	2.5(38.46)	4	5(125)

Claims (9)

1. a growing swine compound enzyme, be made up of the raw material of following parts by weight: zytase 3-8, cellulase 8-15, beta-glucanase 5-10, mesophilicα-diastase 3-5, tilactase 1-2, polygalacturonase 1-2, plant lactobacillus capsule 3-6, galenical 6-10 part, Rhizoma Zingiberis Recens extract 2-4 part, Radix Glycyrrhizae 1-2 part.

2. growing swine compound enzyme according to claim 1, it is characterized in that, the preparation method of described galenical is as follows:

Take Semen Cassiae 10-18 part; Bark of eucommia 5-15 part; Poria cocos 10-15 part; Respectively said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 3-6 times of weight in container, add the acidic cellulase of said mixture material constituent mass 1-2%, control temperature 45-55 DEG C, pH:3.5-4, keeps 1-2h, adds the mixture of mixture 2-3 times of w ethanol and propyl alcohol subsequently, control temperature to 60 DEG C-78 DEG C of maintenance 3-4h, filter; Filter vacuum concentrates postlyophilization and obtains galenical.

3. growing swine compound enzyme according to claim 1, it is characterized in that, the preparation method of described Rhizoma Zingiberis Recens extract is as follows:

To be crushed to particle diameter is that less than 2 millimeters gingers are placed in container, adds the water of ginger 3-6 times of weight, and control temperature 50 DEG C-60 DEG C keeps 2-3h, adds the mixture of ginger 2-3 times of w ethanol and methyl alcohol, and control temperature to 30 DEG C-40 DEG C of maintenance 3-8h, filter; Filter vacuum concentrates postlyophilization and obtains Rhizoma Zingiberis Recens extract.

4. according to growing swine compound enzyme described in claim 1, it is characterized in that, the preparation method of described plant lactobacillus capsule is as follows:

Plant lactobacillus capsule product is made up of wall material, plant lactobacillus, lyophilized vaccine, stachyose and morel enzymolysis powder;

Described wall material is made up of enzymatic soybean protein isolate, chitosan, xanthan gum, carrageenin, glycerine and trehalose; Concentration when above-mentioned each material is made into mixing solutions is respectively: enzymatic soybean protein isolate 7-10%, chitosan 1-2%, xanthan gum 1-3%, carrageenin 0.1-0.5%, glycerine 0.3-1%, trehalose 0.5-2%; The preparation method of enzymatic soybean protein isolate is: compound concentration is that 10-13% soybean protein isolate solution is heated to 30-45 DEG C, adjustment pH to 3-5, add the aspartic protease insulation enzymolysis 0.5-1.5 hour of soybean protein isolate weight 0.1-1%, after enzymolysis, solution spray drying obtains enzymatic soybean protein isolate.

5. according to growing swine compound enzyme described in claim 4, it is characterized in that, the preferred CGMCC NO.9405 of plant lactobacillus.

6. according to growing swine compound enzyme described in claim 1-4, it is characterized in that, preparation method is as follows for plant lactobacillus capsule product:

1) core is prepared: in the plant lactobacillus fermented liquid cultivated through ferment tank, add the stachyose of fermented liquid quality 3-5% and the morel enzymolysis powder of 5-8%, then mix with frozen-dried protective agent solution, pre-freeze 0.5h at-50 DEG C, then freeze-drying 10-18h in vacuum freeze drier, grinds after freeze-drying and makes core;

The ratio of described fermented liquid and frozen-dried protective agent solution is 1:1.4-0.8; Described frozen-dried protective agent solution is made up of skim-milk, trehalose and maltodextrin solution; The volume ratio of three kinds of solution is skim-milk: trehalose: maltodextrin=3:1:0.5;

The concentration of described skim-milk, trehalose and maltodextrin solution is respectively: skim-milk 5%, trehalose 1%, maltodextrin 2%;

2) bag quilt: core is suspended in fluidized-bed, wall material spraying bag quilt, the mixed solution of chitosan, glycerine and trehalose is sprayed into from a shower nozzle, the mixed solution of xanthan gum, carrageenin and enzymatic soybean protein isolate is sprayed into from another shower nozzle, spray velocity controls identical, bag between 25-38 DEG C, is namely formed capsule after 30 minutes by temperature in fluidized-bed in process.

7. according to growing swine compound enzyme described in claim 1, it is characterized in that, described mesophilicα-diastase has following characteristic:

(1) this enzyme thermal adaptation a wider range, temperature activity between 55-75 DEG C is higher, and optimum temperature is 65 DEG C;

Thermostability: this enzyme is preserved 24h and still had the work of 80-83% enzyme under 65 DEG C of conditions, preserves 12h and still has the work of more than 50-60% enzyme under 70 DEG C of conditions;

(2) this enzyme optimal reaction pH value is 5.0, between pH value 4.0-6.0, all have high enzyme vigor; Acid acceptance: still have the enzyme of more than 80% to live preserve 18h under pH4-6 after;

(3) enzymic activity: bacterial strain 304 is 7000-9600U/ml by the acid mesophilicα-diastase enzyme activity that fermentation is standby; Be particularly suitable for liquefaction process and Mashing process and the industrialization demand of depositing; The bacterial strain producing mesophilicα-diastase is specially subtilis 304Bacillus subtilis304, and preserving number is CCTCC NO:M 2013600.

8. according to growing swine compound enzyme described in claim 1, it is characterized in that, be made up of the raw material of following parts by weight: zytase 3, cellulase 10, beta-glucanase 8, mesophilicα-diastase 4, tilactase 1, polygalacturonase 1, plant lactobacillus capsule 5, galenical 8 parts, Rhizoma Zingiberis Recens extract 3 parts, 1.5 parts, Radix Glycyrrhizae;

Preferably, described growing swine compound enzyme, is made up of the raw material of following parts by weight:

Zytase 8, cellulase 15, beta-glucanase 5, mesophilicα-diastase 5, tilactase 2, polygalacturonase 2, plant lactobacillus capsule 3, galenical 10 parts, Rhizoma Zingiberis Recens extract 2 parts, 2 parts, Radix Glycyrrhizae;

Preferably, described growing swine compound enzyme, is made up of the raw material of following parts by weight:

Zytase 5, cellulase 8, beta-glucanase 10, mesophilicα-diastase 5, tilactase 1, polygalacturonase 1, plant lactobacillus capsule 3, galenical 6 parts, Rhizoma Zingiberis Recens extract 2 parts, 1 part, Radix Glycyrrhizae.

9. according to the preparation method of growing swine compound enzyme described in claim 1, as follows:

By described Radix Glycyrrhizae, galenical and Rhizoma Zingiberis Recens extract micronizing respectively, then with zytase, cellulase, beta-glucanase, amylase, tilactase, polygalacturonase, packs the growing swine compound enzyme that gets product after plant lactobacillus capsule Homogeneous phase mixing.