CN104621547A - Ultra-fine cordyceps-guangdongensis fruiting body powder with high absorption ratio - Google Patents
Ultra-fine cordyceps-guangdongensis fruiting body powder with high absorption ratio Download PDFInfo
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- CN104621547A CN104621547A CN201510024553.5A CN201510024553A CN104621547A CN 104621547 A CN104621547 A CN 104621547A CN 201510024553 A CN201510024553 A CN 201510024553A CN 104621547 A CN104621547 A CN 104621547A
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- 239000000843 powder Substances 0.000 title claims abstract description 16
- 241001549246 Cordyceps guangdongensis Species 0.000 title claims abstract description 8
- 238000010521 absorption reaction Methods 0.000 title abstract description 7
- 239000002245 particle Substances 0.000 claims abstract description 5
- 241001264174 Cordyceps militaris Species 0.000 claims description 22
- 238000000227 grinding Methods 0.000 claims description 8
- 230000008901 benefit Effects 0.000 abstract 2
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 19
- 238000000034 method Methods 0.000 description 11
- 230000029087 digestion Effects 0.000 description 9
- 238000004088 simulation Methods 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 6
- 239000004480 active ingredient Substances 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000003716 rejuvenation Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 240000001307 Myosotis scorpioides Species 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000190633 Cordyceps Species 0.000 description 1
- 208000002979 Influenza in Birds Diseases 0.000 description 1
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 240000006698 Spigelia anthelmia Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- 208000020832 chronic kidney disease Diseases 0.000 description 1
- 208000022831 chronic renal failure syndrome Diseases 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000001175 peptic effect Effects 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses ultra-fine cordyceps-guangdongensis fruiting body powder with high absorption ratio. The ultra-fine powder is obtained by ultra-fine crushing of cordyceps-guangdongensis fruiting bodies. The ultra-fine powder disclosed by the invention has the advantages that the cell walls of the cordyceps-guangdongensis fruiting bodies can be quickly destructed by ultra-fine crushing, so that the dissolving speed of effective components is increased, simultaneously the particle diameter is uniform, and further the benefit for absorption of the gastrointestinal tract is achieved; the effective components can be prevented from being destructed in the long-time extraction process, energy can be greatly saved and the utilization rate of the ultrafine powder for the human body can be increased.
Description
Technical field:
The invention belongs to microbial technology field, be specifically related to a kind of Guangdong Cordyceps militaris fruiting body Ultramicro-powder with high-absorbility.
Background technology:
Modern scientific research shows, Chinese caterpillar fungus has very high nutritive value, and along with the raising day by day of people's living standard, also constantly expands the demand of worm grass product.Due to natural cs resource scarcity, expensive, artificial cultivation Chinese caterpillar fungus has become popular.Guangdong Cordyceps militaris (Cordyceps guangdongensis T.H.Li, Q.Y.Lin & B.Song) be that Guangdong Microbes Inst finds and the new varieties of success domestication in recent years, its fructification and cordyceps sporophore plesiomorphism, safety non-toxic and can artificial cultivation, and be approved as new resource food by the Ministry of Public Health; The content of the active component containing multiple Cordyceps sinensis, particularly cordycepic acid is particularly remarkable; There is antifatigue and delay senility, anti-oxidant, anti-avian influenza virus, the effect such as control chronic renal failure, utilize its development function new product to have wide market prospects.Remarkable as a kind of effect, safe and reliable new resource food, it contains the nutrient of multiple needed by human, these nutrients only have digested after, its effect of competence exertion.Therefore, the absorptivity how improving product Middle nutrition material is very crucial.
Summary of the invention:
The object of this invention is to provide a kind of Guangdong Cordyceps militaris fruiting body Ultramicro-powder with high-absorbility.
The Guangdong Cordyceps militaris fruiting body Ultramicro-powder with high-absorbility of the present invention, it is characterized in that, that dry Guangdong Cordyceps militaris (Cordyceps guangdongensis T.H.Li, Q.Y.Lin & B.Song) fructification is obtained after ultramicro grinding.
Preferably, Guangdong Cordyceps militaris fruiting body is obtained after ultramicro grinding to particle diameter is 200 orders.
The present invention can the cell membrane of rapid damage Guangdong Cordyceps militaris fruiting body by ultramicro grinding, and make active ingredient enhanced dissolution rate wherein, uniform particle sizes, is conducive to GI absorption simultaneously; Active ingredient can be avoided again destroyed in long-time leaching process, can greatly economize energy, improve human body to its utilization rate.
Detailed description of the invention:
Following examples further illustrate of the present invention, instead of the restriction to invention.
Embodiment 1:
(1) wild Guangdong Cordyceps militaris (Cordyceps guangdongensis T.H.Li, Q.Y.Lin & B.Song) fructification is carried out tissue to be separated, cultivate on PDA culture medium, obtain pure bacterial strain; For preventing strain degeneration, conventionally rejuvenation is carried out to bacterial classification;
(2) bacterial classification makes: comprise female kind, level liquid kind and secondary and produce the making of planting.
1. female kind makes: the aseptic rich PDA slant medium that adds of Guangdong Cordyceps militaris bacterial strain access after rejuvenation (by mass fraction, is comprised potato 20%, glucose 2%, KH
2pO
40.3%, MgSO
47H
2o 0.15%, Cobastab
1a little, agar 2%, surplus is water) in, at 20-25 DEG C, after light culture 10-15 days, illumination 4-6 days, chooses the very fast and bacterial classification that mycelial growth is neat of annesl, as mother's kind.
2. level liquid kind makes: the mother selected through rejuvenation planted and be connected to aseptic liquid nutrient medium (by mass fraction, comprise glucose 1%, peptone 0.5%, Fructus Hordei Germinatus leaches powder 0.3%, yeast extract 0.3%, surplus is water, pH 6.0-6.5) in, shaken cultivation 7-10 days, chooses mycelium pellet size uniformity, diameter is 2-3 millimeter as first class inoculum.
3. secondary is produced and is planted: by level liquid bacterial classification (3%-10% by a certain percentage, V/V) aseptic liquid nutrient medium is accessed (by mass fraction, comprise glucose 1%, peptone 0.5%, Fructus Hordei Germinatus leaches powder 0.3%, yeast extract 0.3%, surplus is water, pH6.0-6.5) in, cultivate by the training method of level liquid bacterial classification, choose mycelium pellet size uniformity, diameter is 2-3 millimeter to produce kind as secondary, the cultivation for Chinese caterpillar fungus is produced.
(3) raw materials for production preparation: culture medium for cultivating is that the ratio of rice and nutrient solution 1:1-1.5 in mass ratio mixes, nutrient solution prescription is: by mass fraction, comprise glucose 1%, peptone 0.5%, Fructus Hordei Germinatus leaches powder 0.3%, yeast extract 0.3%, surplus is water, pH6.0-6.5, through autoclaving, namely can be used for inoculation after cooling.
(4) go out grass management: in desinfection chamber or superclean bench, produce through aseptic water-reducible secondary in kind of the culture medium for cultivating that access is aseptic, every bottle graft enters 17-22 milliliter.Carry out lucifuge again and cultivate 9-14 days, cover with after culture medium until mycelia, carry out illumination cultivation, light intensity 400-1000Lux, relative air humidity 80-90%, 18-25 DEG C, can fruit body primordium be grown through 7-12 days, then cultivate 40-50 days.
(5) gather: treat that fructification top becomes circular, and when there is graininess point, can gather.
(6) sample preparation: Guangdong Cordyceps militaris fruiting body is through 60 DEG C of oven dry, the sample (sample 1) of (80 order) is pulverized in contrast with normal conventional, the sample of ultramicro grinding (200 order) as sample 2, for absorption rate testing.
Above-mentioned Fructus Hordei Germinatus used leaches powder and yeast extract is all commercially available product.
Tissue described in step (1) is separated and conventional method can be adopted to carry out, and the rejuvenation described in step (1) is to prevent strain degeneration, and conventional method can be adopted to carry out.
(7) absorption rate testing:
1. stomach simulation digestion phase
Be the hydrochloric acid solution of 1.5 by 0.25g sample (sample 1 or sample 2) and 25mL pH, join in 50mL centrifuge tube, pH is regulated to be 1.5 with 1mol/L hydrochloric acid solution, add 0.16g/mL pepsin liquid 0.5mL again, screw, and the 100min that vibrates under closed environment (37 ± 1 DEG C, 80r/min, lower same), obtain stomach simulation digest.
2. small intestine simulation digestion phase
After the peptic digest stage terminates, simulating digest pH with 1mol/L sodium bicarbonate solution titration stomach is 5.0, then pancreatic juice-cholate mixture 2.5mL is added, persistent oscillation 100min, finally pH is regulated to be 7.2 with 0.2mol/L sodium hydroxide solution, the centrifugal 10min of 5000r/min, obtains sample 1 and sample 2 simulates postdigestive supernatant for detecting.
3. the detection of cordycepic acid content
Utilize high performance liquid chromatography, respectively the cordycepic acid content of the supernatant of sample 1 and sample 2 after the cordycepic acid content of the Guangdong Cordyceps militaris fruiting body without simulation digestion process, simulation digestion is measured.
4. the calculating of bioavailability
This research is compared to Absorbable rod cordycepic acid contained in supernatant after simulation digestion process and utilizes part, and the cordycepic acid quality that Absorbable rod is utilized and the ratio without the cordycepic acid total amount contained by the Guangdong Cordyceps militaris fruiting body of simulating digestion process are as its bioavailability.Relevant computational methods are as follows:
Formula 1: the quality Q 1 (g) of cordycepic acid contained in supernatant after the simulation digestion process of sample 1 equals the volume V1 (mL) that its supernatant cordycepic acid content C1 (g/100mL) is multiplied by supernatant, namely; Q1=C1*V1.
Formula 2: the quality Q 2 (g) of cordycepic acid contained in supernatant after the simulation digestion process of sample 2 equals the volume V2 (mL) that its supernatant cordycepic acid content C2 (g/100mL) is multiplied by supernatant, namely; Q2=C2*V2.
Formula 3: without simulation digestion process Guangdong Cordyceps militaris fruiting body raw sample contained by cordycepic acid total amount Qt (g) equal the weight Wt (g) that its cordycepic acid relative amount Rt (%) is multiplied by fructification, namely; Qt=Rt*Wt.
Formula 4: sample 1 bioavailability B1 (%) is its cordycepic acid quality Q 1 (g) and the ratio of raw sample cordycepic acid total amount Qt (g), that is: B1=Q1/Qt.
Formula 5: sample 2 bioavailability B2 (%) is its cordycepic acid quality Q 1 (g) and the ratio of raw sample cordycepic acid total amount Qt (g), that is: B1=Q2/Qt.
5. interpretation of result:
Above-mentioned test is with the significant cordycepic acid of content in Guangdong Cordyceps militaris fruiting body for index composition detects, and comparative analysis superfine communication technique and Ordinary pulverization technology are on the impact improving active ingredient bioavailability in Guangdong Cordyceps militaris fruiting body.
Can obtain according to after above formula scales, B1 is 1.797, B2 is 2.499, then B2/B1=1.3907.In sum, the bioavailability of known sample 2 is higher than the bioavailability of sample 1 by 39.07%.
From the results of view, superfine communication technique significantly can accelerate the bioavailability of cordycepic acid in Guangdong Cordyceps militaris fruiting body, and compared with Ordinary pulverization sample, the bioavailability of ultramicro grinding sample improves 39.07%.
Therefore, the Guangdong Cordyceps militaris fruiting body Ultramicro-powder with high-absorbility of the present invention, can the cell membrane of rapid damage sample by ultramicro grinding, and make active ingredient enhanced dissolution rate wherein, uniform particle sizes, is conducive to GI absorption simultaneously; Active ingredient can be avoided again destroyed in long-time leaching process, can greatly economize energy, improve human body to its utilization rate.
Claims (2)
1. one kind has the Guangdong Cordyceps militaris fruiting body Ultramicro-powder of high-absorbility, it is characterized in that, that Guangdong Cordyceps militaris (Cordyceps guangdongensis T.H.Li, Q.Y.Lin & B.Song) fructification is obtained after ultramicro grinding.
2. the Guangdong Cordyceps militaris fruiting body Ultramicro-powder with high-absorbility according to claim 1, is characterized in that, is obtained by Guangdong Cordyceps militaris fruiting body after ultramicro grinding to particle diameter is 200 orders.
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| CN201510024553.5A CN104621547A (en) | 2015-01-16 | 2015-01-16 | Ultra-fine cordyceps-guangdongensis fruiting body powder with high absorption ratio |
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| CN201510024553.5A CN104621547A (en) | 2015-01-16 | 2015-01-16 | Ultra-fine cordyceps-guangdongensis fruiting body powder with high absorption ratio |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113975303A (en) * | 2021-11-02 | 2022-01-28 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Application of Cordyceps guangdongensis in preparation of product for preventing or treating intestinal dysbacteriosis or its related diseases |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6474964A (en) * | 1987-09-16 | 1989-03-20 | Takuyoshi Nakamura | Preparation of food containing koji blended with vegetable oil |
| CN1151892A (en) * | 1996-10-21 | 1997-06-18 | 汪景山 | Superfine plant worm powder and its product |
| CN101830753A (en) * | 2010-05-06 | 2010-09-15 | 广东省微生物研究所 | High-yield culture medium of Guangdong Cordyceps militaris fruiting body |
| CN102228245A (en) * | 2011-06-24 | 2011-11-02 | 江苏康迪生物科技有限公司 | Granular preparation prepared from ultramicro pure cordycep sporocarp powder and preparation method thereof |
| CN102687853A (en) * | 2012-06-19 | 2012-09-26 | 福建农林大学 | Preparation method of cordyceps militaris bacterial powder and application thereof |
| CN102893807A (en) * | 2012-09-29 | 2013-01-30 | 广州大光制药有限公司 | Cordyceps militaris (L) link as well as preparation method and application thereof |
| CN103340406A (en) * | 2013-07-17 | 2013-10-09 | 浙江省磐安县外贸药业有限公司 | Preparation method of cordyceps sinensis granules |
| CN104172162A (en) * | 2014-08-06 | 2014-12-03 | 赵胜华 | Post-harvesting processing method of cordyceps militaris |
-
2015
- 2015-01-16 CN CN201510024553.5A patent/CN104621547A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6474964A (en) * | 1987-09-16 | 1989-03-20 | Takuyoshi Nakamura | Preparation of food containing koji blended with vegetable oil |
| CN1151892A (en) * | 1996-10-21 | 1997-06-18 | 汪景山 | Superfine plant worm powder and its product |
| CN101830753A (en) * | 2010-05-06 | 2010-09-15 | 广东省微生物研究所 | High-yield culture medium of Guangdong Cordyceps militaris fruiting body |
| CN102228245A (en) * | 2011-06-24 | 2011-11-02 | 江苏康迪生物科技有限公司 | Granular preparation prepared from ultramicro pure cordycep sporocarp powder and preparation method thereof |
| CN102687853A (en) * | 2012-06-19 | 2012-09-26 | 福建农林大学 | Preparation method of cordyceps militaris bacterial powder and application thereof |
| CN102893807A (en) * | 2012-09-29 | 2013-01-30 | 广州大光制药有限公司 | Cordyceps militaris (L) link as well as preparation method and application thereof |
| CN103340406A (en) * | 2013-07-17 | 2013-10-09 | 浙江省磐安县外贸药业有限公司 | Preparation method of cordyceps sinensis granules |
| CN104172162A (en) * | 2014-08-06 | 2014-12-03 | 赵胜华 | Post-harvesting processing method of cordyceps militaris |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113975303A (en) * | 2021-11-02 | 2022-01-28 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Application of Cordyceps guangdongensis in preparation of product for preventing or treating intestinal dysbacteriosis or its related diseases |
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