CN104611407A - Rapid identification of strawberry powdery mildew gene by using comparative genomics - Google Patents
Rapid identification of strawberry powdery mildew gene by using comparative genomics Download PDFInfo
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Abstract
The present invention provides rapid identification of strawberry anti-powdery mildew gene, relates to knowledge of plant comparative genomics, genetics, bioinformatics and other disciplines, and belongs to the plant biotechnology science field. The main steps of the invention comprise: 1) downloading the complete genome sequence of the strawberry, and collecting MLO type gene; 2) identifying the MLO type gene; 3) achieving the MLO type gene phylogeny relationship; and 4) comparing the MLO type powdery mildew gene. According to the invention, the strawberry powdery mildew gene finding period is effectively shortened so as to easily achieve rapid powdery mildew gene identification; the corresponding co-segregation function marker (SNP, SCAR and the like) is developed through the identified candidate powdery mildew gene, the strawberry anti-powdery mildew gene can further be rapidly used for the molecule marker-assisted selection of the anti-powdery mildew gene, and the accuracy is high; the strawberry anti-powdery mildew gene can be combined with other disease resistance gene molecule markers so as to create multi-resistance breeding materials, shorten the breeding period, and improve the breeding efficiency; and the foundation is established for exposition of the strawberry anti-powdery mildew molecule mechanism.
Description
Technical field
The present invention is by means of strawberry order-checking whole genome sequence, utilize the method Rapid identification powdery mildew of strawberry genes such as plant comparative genomics, genetics, information biology and candidate gene strategy, be mainly concerned with the download of strawberry whole genome sequence, the qualification of candidate gene, the comparison of gene, the means such as cluster, and then identify powdery mildew gene, belong to Plant Biotechnology scientific domain.
Background technology
Powdery mildew of strawberry is one of disease main in strawberry production, this disease is caused by the garment or robe made of feathers single softgel shell bacterium of grass (Sphaerotheca aphanis) of fungi Ascomycotina infects, pathogenic bacteria is obligate parasite, carries out medium and long distance propagation mainly through propaguluies such as the Strawberry Seedlings that carries disease germs; Florescence catches an illness and petal can be caused to redden, and bud is not bloomed, and after fruit is caught an illness, young fruit can not normally expand, and fruit face is covered with meal, has had a strong impact on the quality of strawberry.Powdery mildew of strawberry is once big area occurs, and extremely difficult elimination, strawberry is originally as eating berry raw, and the frequent agricultural chemicals that uses will certainly have influence on edible safety.Therefore, seed selection mildew-resistance has again the kind of the commodity economy proterties such as excellent color, shape, has become the important directions of Strwberry Breeding.
There is larger difference to powder mildew resistance in strawberry cultivars different at present, utilizes traditional breeding method, and intricate operation, breeding cycle are long and efficiency of selection is low, are unfavorable for that seed selection is fast applicable to strawberry disease-resistant variety.So people use modern gene clone technology usually, the disease-resistant gene of external source is imported the excellent strawberry cultivars of proterties, thus obtain the New Strawberry Variety of high resistance high-quality.Therefore clone strawberry mildew-resistance gene and study its structure and functional characteristics, the utilization of enantiopathy gene has great importance.
MLO type disease-resistant gene is the special class disease-resistant gene of plant.Investigator find the earliest MLO (
mildew resistance
locus
o) gene pairs powder mildew resistance starts from 1937-1938, acquired the barley of a lot of kind in Ethiopia by fritz, two strains wherein all have efficient resistance to all known physiological strains of Powdery Mildew (Blumeriagraminisf.sp.hordei).Further research shows, in barley, the recessive mutation mlo of MLO gene can make barley produce resistance that is lasting, wide spectrum to the physiological strain of nearly all known barley powdery mildew bacteria.Recently, investigator finds that the mildew-resistance gene of a lot of plant is all MLO type Gene Handling, as tomato, and pea, Arabidopis thaliana, rose, capsicum, Root or stem of Littleleaf Indianmulberry etc.Therefore the MLO type disease-resistant gene excavated in plant has important effect to Biology of Plant-Powdery Mildew Interaction breeding.
At present, the conventional method excavating disease-resistant gene conventional has map based cloning, the methods such as transposon tagging.But because the fundamental research of strawberry is not deep enough, not only the time is grown but also is difficult to clone these genes exactly therefore to utilize these methods.Therefore, the MLO type disease-resistant gene how in Rapid identification strawberry will become the important prerequisite of strawberry mildew-resistance breeding.
Plant comparative genomics (Comparative Genomics) is based on Genome Atlas and order-checking basis, compares, understand the subject of the function of gene, expression mechanisms and spore to known gene and genome structure.Between Land use models Plant Genome and other Plant Genome on coded sequence and structural homology, clone's other plant gene, discloses gene function and molecular mechanism, illustrates spore relation and genomic immanent structure.The method that this patent adopts and thinking: the research of model plant arabidopsis gene group has disclosed the function of MLO type gene, utilize the homology clone strawberry MLO type disease-resistant gene in its order of gene, according to the feature of the superiority in model plant Arabidopis thaliana experimental system and known MLO type disease-resistant gene, " seizure " strawberry mildew-resistance gene fast.This patent describes premised on strawberry whole genome sequence, in conjunction with knowledge such as comparative genomics, genetics, genomics, information biology and candidate gene strategy, excavates powdery mildew gene fast.
Summary of the invention
Technical problem
The object of this invention is to provide a kind of by conjunction with knowledge such as plant comparative genomics, plant genetics, genomics and information biology, excavate strawberry mildew-resistance gene fast.Its result can be used for the exploitation of powdery mildew of strawberry gene compact linkage molecule mark on the one hand, carries out molecular marker assisted selection breeding, on the other hand also for other crop powdery mildew gene identification provide reference frame.
Technical scheme
Cardinal principle: first Biology of Plant-Powdery Mildew Interaction gene (MLO) is cloned from barley, research finds that this gene is the special disease-resistant gene of a class, is different from most of NBS (nucleotide-binding site) the type disease-resistant gene of previously clone; Subsequently, investigator has cloned powdery mildew gene in succession from the plants such as tomato, Arabidopis thaliana, pea, capsicum, Root or stem of Littleleaf Indianmulberry, and what research found these genes encodings is all MLO type disease-resistant gene.Subsequently, by test of many times, numerous investigator confirms that MLO type disease-resistant gene has become the distinctive class mildew-resistance gene of plant.Further discovery, plant MLO type gene is a gene family; And Phylogenetic Relationships analysis discovery is carried out to the MLO gene family deriving from different plant species, in different plant species mildew-resistance gene always cluster together, become a class, this class MLO gene all has the characteristic feature of mildew-resistance gene sequence.Completing of strawberry gene order-checking provides a convenient approach for excavating powdery mildew gene.Therefore, can by means of the Phylogenetic Relationships of MLO gene family in the strawberry full-length genome checked order and the MLO powdery mildew gene of having cloned and for maintaining the conservation of amino acids of powdery mildew gene MLO critical function to identify powdery mildew of strawberry gene.
Key step is as follows:
1) download of strawberry whole genome sequence and the collection of MLO type gene thereof
First strawberry whole genome sequence is downloaded from strawberry sequenced genes group database (http://www.strawberrygenome.org/); Use " DNATOOLS " software to the strawberry full-length genome amino acid sequence data building database obtained, then pfam database (protein family database is used, http: ∥ pfam.janelia.org/search/sequence) in hidden Markov model (HMM) Blastp (E-value=0.001) sequence alignment is carried out to the aminoacid sequence of MLO structural domain and the strawberry full-length genome amino acid sequence database to have set up, preliminary screening goes out candidate gene sequence.Secondly, utilize the MLO type gene order announced, BLAST comparison is carried out to strawberry genome database, obtain candidate gene sequence.
2) qualification of strawberry MLO type gene family
By the candidate gene of homologous nucleotide sequence obtained in the above results, analyzed by Pfam (E-value=1.0), remove the gene order (Fig. 1) without ' MLO ' structural domain.The ClustalW instrument (Multiple Sequence Alignment program) Candidate Disease Resistant Genes sequence provided by MEGA3.1 software again carries out Multiple Sequence Alignment, removes tumor-necrosis factor glycoproteins.
3) by the strawberry MLO type powdery mildew gene of the Phylogenetic Relationships qualification candidate of plant MLO type gene
Because previous research is verified, dicotyledons MLO type powdery mildew gene is positioned at plant MLO Phylogenetic Tree same district group, therefore in Phylogenetic Relationships research, we are MLO type mildew-resistance gene with strawberry MLO type gene together with the cluster analysis of the MLO type gene family of Arabidopis thaliana with some other crops, to obtain the strawberry mildew-resistance gene (Fig. 2) of candidate.
4) comparison of powdery mildew of strawberry gene and known plant MLO powdery mildew gene
BioXM2.6 software is utilized to convert the aminoacid sequence of the MLO powdery mildew gene of the MLO type powdery mildew gene of strawberry candidate and Arabidopis thaliana, tomato, pea, barley the file of Fasta form to, these files are imported BioEdit7.0 software, use Clustal software in this software to carry out Multiple Sequence Alignment, disclose the conservative property in candidate's powdery mildew gene important amino acid residue and region.Thus identify the powdery mildew gene (Fig. 3) of strawberry candidate further.
Positively effect of the present invention:
1) shorten the powdery mildew of strawberry gene excavating cycle, be conducive to the Rapid identification of powdery mildew gene.Mildew-resistance gene not only takes time and effort, efficiency is low to adopt ordinary method (map based cloning, transposon tagging etc.) to excavate, and is difficult to successfully.The present invention is based on plant comparative genomics, genetics, bioinformatics method and excavate powdery mildew of strawberry gene fast, not only can shorten the time, powdery mildew gene determination rates can also be improved.
2) strawberry (Fragaia ananassa) belongs to is to Rosaceae strawberry plants, is one of the whole world important fruit crop of extensively planting.Because strawberry hereditary basis is narrow, Germplasm Resources Diversity is low, therefore by conventional molecule marker (RAPD, ISSR, SSR, AFLP etc.) qualification powdery mildew of strawberry genetic comparison difficulty.The candidate's powdery mildew gene exploitation be tested and appraised is divided into from functional indicia (SNP, SCAR etc.) accordingly, can fast for the molecular marker assisted selection of disease-resistant gene, carry out the initiative of multiresistance breeding material, can shortening the breeding cycle, improve breeding efficiency.
3) lay a good foundation for setting forth strawberry mildew-resistance molecular mechanism.The qualification of strawberry mildew-resistance gene, by transgenic technology, RNAi, virus induced gene silencing (virus induced gene silencing, VIGS) molecular mechanism of the Effect of Anti Powdery Mildew such as technology provides genetic resources, is conducive to the mechanism of action setting forth strawberry mildew-resistance fast.
Accompanying drawing explanation
The qualification of Fig. 1 strawberry MLO gene;
What this figure showed is 20 MLO type gene identification results, and each gene is containing ' MLO ' conserved domain.
The Phylogenetic Relationships analysis of Fig. 2 plant MLO gene family and the qualification of strawberry MLO type powdery mildew gene thereof;
Arabidopis thaliana is the model plant of plant science research, in phylogenetic tree construction, the powdery mildew gene (PsMLO) of 15 MLO type genes (wherein 3 genes are powdery mildew genes: AtMLO02, AtMLO06 and AtMLO12) of Arabidopis thaliana, Gene against Powdery Mildew in Tomato (SlMLO), barley powdery mildew gene (HvMLO and HvMLO02) and pea is selected to and the analysis of strawberry MLO type gene clusters.Identify the MLO type powdery mildew gene of 3 strawberry candidates altogether.In figure, the gene of italic mark is exactly candidate's powdery mildew of strawberry gene.
The compare of analysis of Fig. 3 strawberry MLO type powdery mildew gene;
3 powdery mildew of strawberry genes and barley (HvMLO), tomato (SlMLO), pea (PsMLO), Arabidopis thaliana powdery mildew gene (AtMLO02, AtMLO06 and AtMLO12) to compare, qualification powdery mildew infects the conservative type in amino-acid residue and the region played an important role.In figure, TM1-TM7 represents 7 revolving die regions of strawberry MLO type powdery mildew gene; Black round dot represents that powdery mildew infects important amino-acid residue; CaMBD represents calmodulin CaM binding region; I and II represents and infects important amino acid region to powdery mildew.
Embodiment
The qualification of disease-resistant gene has important effect in the research of crop disease-resistant theory of heredity and disease-resistant variety seed selection.Present method Rapid identification can go out powdery mildew of strawberry gene.Specific implementation process is as follows:
1) collection of strawberry MLO type gene
In order to obtain the whole MLO type gene family member of strawberry, we are first with the MLO type gene of Arabidopis thaliana, the mildew-resistance MLO gene order of tomato, pea, rose, capsicum, Root or stem of Littleleaf Indianmulberry builds HMM model, receives rope MLO type gene from strawberry genome sequence, secondly the MLO gene order delivered in Different Crop as target sequence (from DFCI database: TC171015, TC267529, DFCI:TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325903, TC315944, TC315912, TC322759, TC322059, TC330654, TC282713, TC293173, TC281861, TC283253, TC283383, TC285032, TC290021, TC302716, TC283487, TC282866, TC283441, TC281428, TC285118, TC285090, from GenBank database: AY967408, AF384145, AF384144, AY029312-AY029315, AY029317-AY029319, Z95352, AF369563-AF369565, AF369567, AF369569-AF369576, Z83834, Z95496, AY581255), BLAST comparison is carried out to strawberry database (http://www.strawberrygenome.org/), select the highest sequence of similarity to download, obtain the MLO type gene (gene09623-v1.0-ab_initio of 20 candidates altogether, gene02355-v1.0-ab_initio, gene02764-v1.0-ab_initio, gene23134-v1.0-ab_initio, gene29691-v1.0-ab_initio, gene09621-v1.0-ab_initio, gene31405-v1.0-ab_initio, gene12974-v1.0-ab_initio, gene10531-v1.0-ab_initio, gene26356-v1.0-ab_initio, gene28466-v1.0-ab_initio, gene14544-v1.0-ab_initio, gene31395-v1.0-ab_initio, gene29204-v1.0-ab_initio, gene31172-v1.0-ab_initio, gene10134-v1.0-ab_initio, gene03198-v1.0-ab_initio, gene10332-v1.0-ab_initio, gene10314-v1.0-ab_initio, gene10995-v1.0-ab_initio).
2) qualification of strawberry MLO type gene conserved domain
In order to verify these MLO gene accuracys further, we to the MLO gene of these 20 candidates carried out conserved domain " MLO ' ' qualification.With the aminoacid sequence of the MLO type gene of each candidate for benchmark, PFAM (http://pfam.sanger.ac.uk/) website is carried out the qualification of ' MLO ' conserved domain, result shows, each MLO type gene has one or more ' MLO ' structural domain (Fig. 1).
3) the Phylogenetic Relationships analysis of strawberry MLO type gene
In previous research, find that dicotyledons powdery mildew gene aggregates into district's group; Therefore, in phylogenetic tree construction, we have selected 15 MLO type genes (wherein 3 genes are powdery mildew genes: AtMLO02, AtMLO06 and AtMLO12) of model plant Arabidopis thaliana, the powdery mildew gene of Gene against Powdery Mildew in Tomato, barley powdery mildew gene and pea analyze with strawberry MLO type gene clusters together with cluster analysis.Strawberry MLO type gene and other crop powdery mildew gene protein sequences are carried out multisequencing connection to join (adopting Clustal X1.83 software to carry out), and utilize Genedoc software (http://www.nrbsc.org/gfx/genedoc/index.html) to show multisequencing to join the result of joining.Clustal multisequencing is joined the result of joining output in MEGA4.0 software, and utilize this software to construct adjacent tree (neighbor-joining, NJ) respectively, utilize Bootstrapping method to assess these evolutionary trees.Found that in dicotyledons mildew-resistance gene district group, there is the MLO type powdery mildew gene (see Fig. 2) of 3 strawberry candidates.
4) comparison of strawberry MLO type disease-resistant gene
In the research of barley MLO type powdery mildew gene, in succession found some important areas and single amino acids in research, they infect large wheat powdery mildew irreplaceable effect.In order to identify in the powdery mildew of strawberry gene of 3 candidates, these important areas and amino acid whether high conservative, we have carried out aligned amino acid sequence analysis to from 3 mildew-resistance genes (AtMLO02, AtMLO6 and AtMLO12) of Arabidopis thaliana, tomato powdery mildew gene (SlMLO), powdery mildew of pea gene (PsMLO).Find the powdery mildew gene of strawberry 3 candidates and known MLO type powdery mildew gene 7 cross-film districts, 30 important amino-acid residue, 1 calmodulin CaM binding region (CaMBD) and two regions played an important role with powdery mildew identification (I and II) high conservative (Fig. 3).Show that these three genes are exactly strawberry MLO type mildew-resistance gene.
Claims (2)
1. strawberry mildew-resistance gene, it is characterized in that being selected from following 3 genes or its one of:
gene09623-vl.0-ab_initio
Aminoacid sequence:
MAAAGKERSLEQTPTWAVAVVCFILVLVSIIIEYILHLIGKWLERKNKRALYEALEKIKAELMLLGFLSLLLTVGQGPISDICISKSVGATWHPCSKKQEPVYAKEDGESTSSESDDDSGRRRLLSVVLSSSGKNARRVLAAAGYDKCGAKGKVPFVSFYGIHQLHILIFVLAVSHVLYCIITLVLGRAKMRKWKTWELETRTADYQFSHDPERFRFARDTSFGRRHLSFWSRSPILLWIVCFFRQFVRSVPKVDYLTLRNGFIFAHLAPQSQTKFDFQKYINRSVEEDFKVVVGISPVIWLFAVLFLLSNTHGSWSYLYLPFIPLVMILLVGTKLQVIITKMGLKIQERGEVVKGTPLVEPGDHLFWFNSPRLILHIIHFVLFQNAFQLAFFAWTWYEFGLKSCFHDKMEDVVLRITMGVVIQILCSYVTLPLYALVTQMGSTMKPTIFNDRVANAIRKWHHAAKKHVKQSKHSVPTSAPGTPLHGMSPVHLLRHYHSELDMDSMQTLPRMSYAEREGPNESPSHHDTDDVPWSNQGQEEEISAHYQPNSVNNPPPGYGNQIQHEIQIHTKD
Nucleotide sequence:
ATGGCAGCAGCTGGAAAGGAACGATCTTTGGAGCAAACGCCGACGTGGGCCGTTGCCGTTGTTTGTTTCATATTGGTTTTGGTCTCCATCATCATCGAATATATCCTTCATTTGATCGGAAAGTGGTTGGAAAGGAAGAATAAAACGAGCTCTCTATGAAGCACTGGAAAAGATCAAAGCAGAGCTTATGTTATTGGGGTTCCTCTCGCTGCTGCTAACAGTAGGACAAGGCCCCATTTCGGACATTTGCATATCGAAGTCTGTTGGGGCTACTTGGCATCCATGTAGTAAGAAGCAAGAGCCAGTCTATGCAAAGGAGGACGGAGAGAGCACTAGTTCAGAATCCGATGATGACAGCGGCCGTAGAAGGCTACTATCCGTGGTTCTATCGAGTTCCGGCAAGAATGCCCGACGTGTTTTGGCCGCAGCCGGATATGACAAATGCGGTGCCAAGGGAAAAGTTCCATTTGTGTCATTTTATGGAATTCACCAGCTTCATATACTCATCTTCGTGCTAGCCGTATCCCATGTGCTTTACTGCATAATAACACTGGTTTTGGGCAGAGCTAAGATGAGGAAGTGGAAAACATGGGAATTGGAAACCAGGACAGCAGACTACCAGTTCTCACATGACCCTGAAAGATTCCGATTTGCGAGAGACACATCGTTTGGGCGAAGGCACTTGAGCTTTTGGAGCCGGTCACCCATTTTGTTATGGATTGTTTGCTTCTTCAGACAGTTTGTTAGATCAGTGCCTAAGGTGGATTACCTGACGCTACGCAATGGGTTCATCTTTGCACATTTGGCACCTCAGAGTCAAACTAAATTTGATTTCCAGAAGTACATCAACAGATCAGTCGAAGAGGACTTCAAGGTTGTGGTGGGGATCAGCCCTGTTATCTGGTTATTCGCCGTATTGTTCCTGCTATCTAACACCCATGGCTCATGGTCCTATCTCTATCTACCTTTTATTCCCCTAGTGATGATCTTGTTAGTAGGGACAAAGCTACAGGTTATAATAACCAAGATGGGGCTTAAAATTCAAGAAAGAGGAGAAGTAGTTAAGGGTACCCCGCTTGTTGAGCCTGGTGACCATCTCTTCTGGTTCAACAGCCCTCGTCTCATTCTTCACATCATCCATTTTGTTCTCTTTCAGAATGCCTTTCAACTTGCTTTCTTTGCATGGACTTGGTACGAATTTGGGTTGAAGTCTTGCTTTCACGACAAGATGGAAGATGTGGTCTTAAGAATTACAATGGGGGTCGTCATACAAATACTATGCAGCTATGTAACTCTACCACTTTATGCCTTAGTTACACAGATGGGTTCAACTATGAAGCCTACGATATTTAACGACAGAGTAGCAAATGCTATAAGAAAGTGGCACCACGCAGCAAAGAAGCATGTAAAACAGAGCAAGCATTCGGTTCCAACCAGTGCACCCGGCACTCCATTACATGGCATGTCCCCGGTTCATCTTCTGCGGCACTATCATTCCGAACTGGACATGGATAGTATGCAAACATTGCCAAGGATGTCATACGCTGAAAGAGAAGGTCCAAACGAGTCCCCATCTCACCACGACACCGACGATGTACCCTGGTCTAACCAGGGTCAGGAGGAAGAGATTAGTGCTCACTATCAACCTAACTCGGTGAACAATCCTCCACCTGGTTATGGAAACCAAATTCAACACGAAATCCAAATTCACACAAAGGATTAG
gene02764-vl.0-ab_initio
Aminoacid sequence:
MAASTSGRSLEQTPTWAVAVVCFVLVLISIIIEHILELIGKWLKNKHKRALFEALEKVKSELMLLGFISLLLTVGQGPISNICISEKVGNTWHPCSKKQEVKIEEEEEVESPSRRLLAAFESGYGGGNVRRVLAAAGTDKCAAKGKVPFISADGIHQLHIFIFVLAIFHVLYCILTMALGRAKVCFFRQFVRSVPKVDYLTLRHGFIMAHLAPQSHQQFNFQKYINRSLEEDFKVVVGISPPIWCFAVIFLLFNTHGWYSYLWLPFIPLIIILLVGTKLQVIITKMGLRIQERGEVVKGTPVVQPGDDLFWFNRPRLILYLINFVLFQNAFQLAFFAWSWYEFSLKSCFHEHIEDIIIRVSMGVLIQILCSYVTLPLYALVTQMGSTMKPTIFNERVATALRKWHHTAKKHIKQNKGSVTPMSSRPGTPSHHASPIHLLRHYRSEVDSFHTSPRRSNFDVEQRYETDSPSPSHHLHIDGSFSHQHQHQVELGNVEHDRMEVNEPNSGHATIRGSQSTREQHEINIGPPKDFSFDKRQAASSSYNMPLVFRLTDPNSMSESEAMVIVEGLLGKLARRCYAVLCLGVPVVSVTVAHFIKGTMDLPEEPTQPRERSKRRLRALQGQLFVPIESSY
Nucleotide sequence:
ATGGCGGCATCAACCTCTGGAAGATCGCTGGAGCAAACGCCCACATGGGCCGTCGCCGTCGTCTGTTTTGTCTTGGTTCTTATCTCAATCATCATCGAGCACATACTCGAACTTTAGGAAAGTGGCTGAAGAATAAACACAAACGAGCTCTATTTGAAGCACTAGAAAAGGTGAAATCAGAGCTTATGCTACTGGGGTTCATATCGCTGCTGCTGACCGTCGGGCAGGGTCCGATATCGAACATATGCATATCGGAGAAAGTGGGGAACACGTGGCATCCGTGTAGCAAGAAGCAGGAGGTTAAGATCGAGGAAGAAGAAGAGGTTGAAAGCCCGAGTAGGAGACTGCTGGCGGCGTTTGAATCTGGTTATGGAGGTGGAAACGTACGCCGCGTTTTGGCGGCTGCTGGCACCGACAAGTGTGCAGCCAAGGGAAAAGTTCCATTCATATCTGCCGATGGTATTCATCAGCTGCATATATTTATCTTCGTCTTGGCTTTTTCCACGTACTCTATTGCATCCTCACAATGGCTTTAGGAAGAGCCAAGGTTTGTTTTTTCAGACAATTTGTTAGATCAGTTCCCAAAGTTGATTACTTGACCTTGCGGCATGGATTTATCATGGCACATTTGGCACCTCAAAGCCATCAACAATTCAATTTCCAGAAGTACATAAACAGATCACTCGAAGAGGATTTCAAAGTTGTTGTGGGAATCAGCCCTCCAATTTGGTGCTTTGCCGTGATCTTTCTACTCTTCAATACTCATGGCTGGTATTCGTATCTTTGGTTACCCTTTATCCCACTCATTATTATTCTATTGGTGGGAACCAAGCTACAAGTGATCATAACCAAGATGGGTCTTAGAATTCAGGAAAGAGGAGAAGTTGTGAAGGGCACACCAGTTGTTCAACCTGGTGATGACCTCTTCTGGTTCAATCGCCCTCGCCTCATTCTCTACCTCATCAACTTCGTTCTCTTTCAGAATGCCTTCCAACTTGCTTTCTTCGCATGGAGTTGGTATGAATTTAGTTTGAAATCTTGCTTTCATGAGCATATTGAGGATATCATCATCAGAGTTTCAATGGGGGTCCTCATACAAATTCTTTGCAGTTATGTCACTCTCCCCCTTTATGCCCTTGTCACACAGATGGGCTCTACTATGAAACCAACAATATTCAACGAGAGAGTAGCCACAGCTCTACGCAAGTGGCATCACACGGCTAAGAAGCACATCAAACAGAACAAAGGCTCCGTCACCCCAATGTCTAGCCGACCAGGCACTCCATCCCACCATGCATCGCCCATCCACCTCCTGCGGCACTACCGCAGCGAGGTGGATAGCTTCCACACATCACCACGGAGATCAAACTTCGACGTCGAACAACGTTACGAAACCGACTCCCCCTCCCCCTCCCACCACCTCCACATAGATGGTTCATTTTCTCACCAACACCAACACCAAGTCGAATTAGGGAATGTGGAACATGATCGGATGGAGGTCAATGAACCCAACTCCGGTCATGCCACAATTAGAGGTTCTCAATCGACTCGCGAACAACACGAAATTAATATCGGACCGCCCAAGGACTTTTCGTTCGATAAGAGACAAGCGGCTTCTTCCTCCTATAATATGCCCCTTGTTTTTCGGCTCACTGATCCAAATTCCATGTCAGAGAGTGAAGCCATGGTGATAGTGGAAGGATTGTTGGGGAAGCTGGCTCGTCGGTGCTATGCGGTGTTGTGTCTGGGGGTGCCGGTTGTGTCGGTGACGGTGGCTCACTTCATTAAAGGAACTATGGATCTGCCGGAGGAACCTACTCAACCTAGAGAGCGCAGCAAGCGCCGTCTTCGAGCTTTGCAGGGTCAGCTCTTCGTACCAATCGAGTCTAGCTACTGA
gene23134-vl.0-ab_initio
Aminoacid sequence:
MAALEYERTLEKTPTWAVAAVCFVLLVISIFIEHVINFIGKWFRSRQKRALYEALEKIKSELMLLGFLSLLLTVLQDPISGICIPKSVAATWHPCNDDPPKTTAGKLEEYSDSGFIFRRQLATKGYDKCTAHGKVAFVSAYGIHQLHIFIFVLAIFHVLYCITTLFLGRHKMRIWKYWENETKTIEYQFSTDPERFRYTRDTSFGKRHLHFWSESPVFLWIVCFFRQFYRSVTKTDYLTLRHGFITAHLAPGSETTFDFRKYISRSLEDDFLVIVEISPIIWFSAVLFLLSNAYGWHSYLWLPFISLIIILVVGTKLQVIITKLGLRIQDRGGVVMGAPLVQPGDQHFWFGRPRFLLFLIHFALFQNAFQLAFFAWSTWQFGITSCFHQTPQDTAIRLSVGVIVQVLCSYVTLPLYALVTQNANNRSEVTDGDDLFPPPDASVVVDMQHWDTEGSRSPKNNVGSSERGGIRQPSTFELGRTQELPAFSLSHPEVSSPSGISSARARK
Nucleotide sequence:
ATGGCGGCACTCGAATATGAGCGTACTTTAGAGAAAACTCCAACATGGGCAGTTGCTGCGGTCTGTTTTGTGTTGCTTGTAATATCAATTTTCATCGAGCATGTCATCAACTTCATAGGAAAGTGGTTTAGAAGTAGACAGAAGCGAGCTCTCTATGAAGCTCTTGAGAAGATCAAATCAGAGCTTATGCTGTTGGGATTCCTATCCTTGCTCCTAACAGTTCTACAAGATCCAATTTCTGGTATATGTATACCAAAGAGTGTTGCAGCCACTTGGCATCCTTGCAATGATGACCCTCCAAAGACTACAGCTGGGAAACTTGAGGAATACTCAGATTCTGGCTTCATTTTCCGGCGCCAATTAGCCACAAAAGGATATGATAAGTGCACGGCTCATGGTAAAGTTGCCTTTGTGTCTGCATATGGGATTCACCAGCTCCATATATTCATCTTTGTGCTAGCAATTTTTCATGTGCTCTACTGCATCACAACTTTGTTTTTGGGAAGACACAAGATGAGAATATGGAAGTATTGGGAGAATGAGACAAAAACAATTGAATACCAGTTTAGTACTGATCCAGAAAGGTTTAGGTATACGAGGGATACATCATTTGGAAAGAGGCATTTACACTTCTGGAGCGAGTCACCAGTTTTCCTTTGGATTGTATGCTTCTTCAGACAGTTCTACAGGTCAGTTACTAAGACTGATTACCTGACTCTGAGGCATGGCTTTATCACGGCACATTTAGCACCTGGGAGTGAAACGACCTTTGATTTTCGGAAGTACATAAGTAGATCACTTGAAGATGACTTCTTAGTTATTGTTGAGATAAGTCCAATTATATGGTTCTCAGCAGTGCTGTTCCTGTTGTCTAATGCATATGGATGGCATTCGTATCTCTGGCTACCATTTATCTCTTTAATTATAATCCTTGTGGTGGGAACTAAACTACAAGTGATTATAACCAAGCTGGGGCTAAGGATTCAGGATAGAGGGGGTGTGGTGATGGGTGCACCGCTTGTTCAACCCGGTGATCAGCACTTCTGGTTTGGACGCCCGCGCTTCTTGCTCTTCCTCATCCACTTTGCTTTATTCCAGAATGCATTTCAGCTGGCCTTCTTTGCATGGAGTACTTGGCAGTTTGGGATAACTTCTTGCTTCCATCAAACCCCTCAAGATACCGCCATCAGGCTCTCAGTGGGGGTTATCGTACAAGTTTTATGTAGTTATGTGACTCTGCCACTCTATGCTCTAGTGACACAGAATGCCAACAACCGCAGTGAGGTCACTGATGGGGATGACCTATTTCCACCTCCAGACGCATCAGTCGTAGTTGATATGCAACATTGGGACACTGAAGGGTCACGTTCCCCGAAGAACAATGTTGGATCGAGTGAAAGAGGAGGAATTAGGCAACCTAGTACTTTTGAGTTAGGCCGAATTCAAGAGCTACCTGCATTCTCCTTGTCTCACCCTGAAGTTAGTAGTCCCTCAGGCATCTCATCTGCTAGAGCAAGAAAGTGA。
2. the application of Rapid identification powdery mildew of strawberry gene described in claim 1, comprising:
1) initiative or the new variety initiative of the breeding material of mildew-resistance MLO gene is carried: utilize the MLO GENE SOURCES given by right 1 to be parent material, in hybridization, backcross progeny, the breeding material that transformation has MLO resistant gene can be obtained.
2) mildew-resistance fundamental research: molecular marker analysis is carried out to the MLO type mildew-resistance gene of right 1; Molecular Mapping or the assignment of genes gene mapping are carried out to this gene; This gene is cloned and Interaction among genes analysis.
3) mildew-resistance transgenic research: utilize the MLO type powdery mildew gene given by claim 1 to carry out transgenic research.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310541421.0A CN104611407A (en) | 2013-11-04 | 2013-11-04 | Rapid identification of strawberry powdery mildew gene by using comparative genomics |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310541421.0A CN104611407A (en) | 2013-11-04 | 2013-11-04 | Rapid identification of strawberry powdery mildew gene by using comparative genomics |
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| Publication Number | Publication Date |
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| CN104611407A true CN104611407A (en) | 2015-05-13 |
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| CN201310541421.0A Pending CN104611407A (en) | 2013-11-04 | 2013-11-04 | Rapid identification of strawberry powdery mildew gene by using comparative genomics |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106096327A (en) * | 2016-06-07 | 2016-11-09 | 广州麦仑信息科技有限公司 | Gene character recognition methods based on the study of the Torch supervised degree of depth |
| CN114395617A (en) * | 2022-01-12 | 2022-04-26 | 天津市农业科学院 | LAMP visual rapid detection method for strawberry powdery mildew and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703463A (en) * | 2012-03-31 | 2012-10-03 | 常熟市支塘镇新盛技术咨询服务有限公司 | Rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing comparative genomics |
| CN102719448A (en) * | 2012-03-31 | 2012-10-10 | 常熟市支塘镇新盛技术咨询服务有限公司 | Application of bioinformatics in fast identifying powdery mildew resistance gene of carica papaya l |
| CN102796745A (en) * | 2012-03-31 | 2012-11-28 | 常熟市支塘镇新盛技术咨询服务有限公司 | Method for quickly identifying manihot esculenta mildew-resistance locus (MLO) gene by applying comparative genomics |
-
2013
- 2013-11-04 CN CN201310541421.0A patent/CN104611407A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703463A (en) * | 2012-03-31 | 2012-10-03 | 常熟市支塘镇新盛技术咨询服务有限公司 | Rapid identification of powdery mildew resistance Lotus corniculatus genes utilizing comparative genomics |
| CN102719448A (en) * | 2012-03-31 | 2012-10-10 | 常熟市支塘镇新盛技术咨询服务有限公司 | Application of bioinformatics in fast identifying powdery mildew resistance gene of carica papaya l |
| CN102796745A (en) * | 2012-03-31 | 2012-11-28 | 常熟市支塘镇新盛技术咨询服务有限公司 | Method for quickly identifying manihot esculenta mildew-resistance locus (MLO) gene by applying comparative genomics |
Non-Patent Citations (4)
| Title |
|---|
| 刘建成等: "草莓抗白粉病遗传图谱及其SSR标记初分析", 《江西农业学报》 * |
| 无: "gene02764-vl.0-ab_initio， fvesca_v1.0_genemark_abinitio.fna", 《HTTP://WWW.ROSACEAE.ORG/TOOLS/NCBI_BLAST》 * |
| 无: "gene23134-vl.0-ab_initio，fvesca_v1.0_genemark_abinitio.fna", 《HTTP://WWW.ROSACEAE.ORG/TOOLS/NCBI_BLAST》 * |
| 无: "XM_004304365.1", 《GENBANK》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106096327A (en) * | 2016-06-07 | 2016-11-09 | 广州麦仑信息科技有限公司 | Gene character recognition methods based on the study of the Torch supervised degree of depth |
| CN106096327B (en) * | 2016-06-07 | 2018-08-17 | 广州麦仑信息科技有限公司 | Gene character recognition methods based on Torch supervised deep learnings |
| CN114395617A (en) * | 2022-01-12 | 2022-04-26 | 天津市农业科学院 | LAMP visual rapid detection method for strawberry powdery mildew and application |
| CN114395617B (en) * | 2022-01-12 | 2023-11-07 | 天津市农业科学院 | LAMP (loop-mediated isothermal amplification) visual rapid detection method and application of powdery mildew of strawberry |
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Application publication date: 20150513 |