CN102703462A - Rapid identification of anti-powdery-mildew gene of Brachypodium distachyon - Google Patents
Rapid identification of anti-powdery-mildew gene of Brachypodium distachyon Download PDFInfo
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Abstract
The invention relates to rapid identification of anti-powdery-mildew gene of Brachypodium distachyon, relates to the knowledge of plant comparative genomics, genetics, biological information and other subjects, and belongs to the scientific field of plant biotechnology. The rapid identification of anti-powdery-mildew gene of Brachypodium distachyon comprises the main steps of: (1) downloading full genome sequences of Brachypodium distachyon and collecting MLO (mildew resistance locus o) type gene; (2) identifying the MLO type gene; (3) analyzing the MLO type gene historical development relation; and (4) comparing the MLO type powdery mildew gene. According to the rapid identification of anti-powdery-mildew gene of Brachypodium distachyon provided by the invention, the excavation period of the anti-powdery-mildew gene of Brachypodium distachyon is effectively shortened, and the rapid identification of the powdery mildew gene is facilitated; the identified powdery mildew gene can be used for developing corresponding coseparation functional markers (SNP (single nucleotide polymorphism), SCAR (sequence-characterized amplified region), and the like), and can also be rapidly used for assisted selection of molecule markers of anti-powdery-mildew gene, and the accuracy is high; development of multi-resistance breeding materials can be carried out by combining with other anti-disease gene molecule markers, the breeding time is shortened and the breeding efficiency is improved; and foundation is established for expounding the molecular mechanism of the anti-powdery-mildew gene of Brachypodium distachyon.
Description
Technical field
The present invention is by means of two fringe false bromegrasses order-checking whole genome sequence, utilizes method Rapid identification two fringe false bromegrass powdery mildew genes such as plant comparative genomics, genetics, information biology and candidate gene strategy; This invention is mainly concerned with the download of two fringe false bromegrass whole genome sequences, the evaluation of candidate gene, and the comparison of gene, means such as cluster, and then identify powdery mildew gene, belong to the Plant Biotechnology scientific domain.
Background technology
Two spike Brachypodium (
Brachypodiumdistachyon ) is a temperate grasses, their growth conditions require relatively simple, small plant, self-pollination, short growth cycle, reproductive ability, easily converted, small genome, genetic resources abundant Studies have shown that two spike Brachypodium and wheat, rice has a common ancestor, which was close to wheat, the two genes were similar at 95%, and susceptible wheat a variety of bacteria to infect two Fringe Brachypodium, so two Fringe Brachypodium is used to study the wheat, rice crops, the ideal of the new model plant plants.
As everyone knows, wheat powdery mildew is a kind of worldwide disease, all can fall ill at each growthdevelopmental stage of wheat; Cause plant early withered when serious, cause the underproduction, even total crop failure; At present, some scholars just are being devoted to the Study on Molecular Marker of wheat anti-powdery mildew property gene both at home and abroad, through seeking and the closely linked molecule marker of disease-resistant gene; Direct or indirect location disease-resistant gene through cloned resistance gene, conversion and accumulation, quickens the process of resist powdery mildew of wheat breeding.Yet the wheat cdna group is bigger, and complexity is high, and for example, the genome of cultivated wheat is approximately 17000Mb, and comprises 3 separate genomes, and this has just hindered the research of wheat being carried out genomics and molecular breeding.Two fringe false bromegrasses and wheat belong to the Gramineae Pooideae together.Currently, the two Sui Brachypodium genome sequencing and notes have been duly completed, which is the first one subfamily grass Poa species to be sequenced.Therefore, biological property, gene expression pattern and the function etc. of the mildew-resistance gene of system summary two fringe false bromegrasses will help to utilize molecular biotechnology to quicken to solve on the cereal crop such as wheat and barley the harm to Powdery Mildew.
MLO type disease-resistant gene is one type of special disease-resistant gene of plant.The investigator find the earliest MLO (
MIldew resistance
lOcus
o ) the gene pairs powder mildew resistance is to start from 1937-1938, gathered the barley of a lot of kinds by the fritz in Ethiopia, two strains systems wherein to the white powder germ (
Blumeria graminisF. sp.
Hordei) physiological strains that all oneself know all have efficient resistance.Further research shows, the recessive mutation mlo of MLO gene can make barley that the physiological strain of nearly all oneself knowledge barley powdery mildew bacteria is produced resistance lasting, wide spectrum in the barley.Recently, the investigator finds that the mildew-resistance gene of a lot of plants all is a MLO type Gene Handling, like tomato, and pea, Arabidopis thaliana, rose, capsicum, Root or stem of Littleleaf Indianmulberry or the like.Therefore breeding has important effect to the plant mildew-resistance to excavate MLO type disease-resistant gene in the plant.
At present, the disease-resistant gene method commonly used of excavating commonly used has map based cloning, methods such as transposon tagging.But because the fundamental research of two fringe false bromegrasses is not deep enough, not only the time is long but also very difficult these genes of cloning exactly therefore to utilize these methods.Therefore, how the MLO type disease-resistant gene in the Rapid identification two fringe false bromegrasses will become the important prerequisite of two fringe false bromegrass mildew-resistance breedings.
Plant comparative genomics (Comparative Genomics) is based on Genome Atlas and the order-checking basis, and known gene and genome structure are compared, and understands the subject of function, expression mechanism and the spore of gene.Utilize between model plant genome and other Plant Genome on the coded sequence and structural homology, clone's other plant gene discloses gene function and molecular mechanism, illustrates spore relation and genomic immanent structure.Method that this patent adopted and thinking: the research of model plant arabidopsis gene group has disclosed the function of MLO type gene; Utilize the homology on its order of gene to clone two fringe false bromegrass MLO type disease-resistant genes; According to the characteristics of meliority on the model plant Arabidopis thaliana experimental system and known MLO type disease-resistant gene, quick " seizure " two fringe false bromegrass mildew-resistance genes.In recent years, the completion of two fringe false bromegrass gene order-checkings provides condition for our fast mining two fringe false bromegrass powdery mildew genes.It is prerequisite that this patent has been introduced with two fringe false bromegrass whole genome sequences, in conjunction with knowledge such as comparative genomics, genetics, genomics, information biology and candidate gene strategy, fast mining powdery mildew gene.
Summary of the invention
Technical problem
The purpose of this invention is to provide a kind of through combining knowledge such as plant comparative genomics, plant genetics, genomics and information biology, fast mining two fringe false bromegrass mildew-resistance genes.Its result can be used for the exploitation of two fringe false bromegrass powdery mildew gene compact linkage molecule marks on the one hand, carries out molecule marker and assists to crops such as wheat transfer mildew-resistance gene, also for other crop powdery mildew gene identification reference frame is provided on the other hand.
Technical scheme
Cardinal principle: first plant mildew-resistance gene (MLO) is cloned from barley, discovers that this gene is one type of special disease-resistant gene, is different from most of NBS (nucleotide-binding site) type disease-resistant gene of previous clone; Subsequently, the investigator has cloned powdery mildew gene in succession from plants such as tomato, Arabidopis thaliana, pea, capsicum, Root or stem of Littleleaf Indianmulberry, and what discover these genes encodings all is MLO type disease-resistant gene.Subsequently, numerous investigators confirm that through test of many times MLO type disease-resistant gene has become one type of mildew-resistance gene of plant specific.Find that further plant MLO type gene is a gene family; And the MLO gene family that derives from different plant species is carried out the Phylogenetic Relationships analysis finds, in the different plant species mildew-resistance gene always cluster become one type together, this type MLO gene all has the characteristic feature of mildew-resistance gene sequence.The completion of two fringe false bromegrass gene order-checkings provides a convenient for excavating powdery mildew gene.Therefore, can identify two fringe false bromegrass powdery mildew genes by means of the Phylogenetic Relationships of MLO gene family in the full genome of two fringe false bromegrasses that has checked order and the MLO powdery mildew gene of having cloned and for the amino acid conservative property of keeping powdery mildew gene MLO critical function.
Key step is following:
1) collection of the download of two fringe false bromegrass whole genome sequences and MLO type gene thereof
At first download two fringe false bromegrass whole genome sequences from two fringe false bromegrass sequenced genes group DBs (http://www.phytozome.net/search.php); Use " DNATOOLS " software that the full genome amino acid sequence data of two fringe false bromegrasses that obtains is set up DB; Use pfam DB (protein family DB then; Http:// pfam.janelia.org/search/sequence) hidden Markov model in (HMM) carries out Blastp (E-value=0.001) sequence alignment to the aminoacid sequence of MLO structural domain with the full genome amino acid sequence database of having set up of two fringe false bromegrasses, and preliminary screening goes out candidate gene sequence.Secondly, utilize the MLO type gene order of having announced, two fringe false bromegrass genome databases are carried out the BLAST comparison, obtain candidate gene sequence.
2) evaluation of two fringe false bromegrass MLO type gene families
With the candidate gene of the homologous nucleotide sequence that obtains in the The above results, analyze through Pfam (E-value=1.0), remove the gene order (Fig. 1) of not having ' MLO ' structural domain.The ClustalW instrument that again the disease-resistant gene sequence is provided through MEGA3.1 software (multisequencing comparison program) carries out the multisequencing comparison, removes Tumor-necrosis factor glycoproteins.
3) identify two fringe false bromegrass MLO type powdery mildew genes through the plant MLO type genic system relation of growing
Because previous research is verified; Dicotyledons MLO type powdery mildew gene is positioned at the same district of plant MLO Phylogenetic Tree group; Therefore in Phylogenetic Relationships research; We are MLO type mildew-resistance gene and the two fringe false bromegrass MLO type gene cluster analyses together of the MLO type gene family of Arabidopis thaliana and some other crops, to obtain two fringe false bromegrass mildew-resistance genes (Fig. 2).
4) comparison of two fringe false bromegrass powdery mildew genes and known plant MLO powdery mildew gene
Utilize BioXM 2.6 softwares the aminoacid sequence of the MLO powdery mildew gene of two fringe false bromegrass candidates' MLO type powdery mildew gene and Arabidopis thaliana, tomato, pea, barley to be converted to the file of Fasta form; These files are imported BioEdit 7.0 softwares; Use in this software Clustal software to carry out the multisequencing comparison, disclose the conservative property in candidate's powdery mildew gene important amino acid residue and zone.Thereby further identify two fringe false bromegrass powdery mildew genes (Fig. 3).
Positively effect of the present invention:
1) shortens two fringe false bromegrass powdery mildew genes and excavated the cycle, helped the Rapid identification of powdery mildew gene.Employing ordinary method (map based cloning, transposon tagging etc.) excavation mildew-resistance gene not only takes time and effort, efficient is low, and is difficult to successfully.The present invention is based on plant comparative genomics, genetics, bioinformatics method fast mining two fringe false bromegrass powdery mildew genes, not only can shorten the time, can also improve powdery mildew gene and identify efficient.
2) two fringe false bromegrasses (
Brachypodium distachyon) belong to the Gramineae false bromegrass and belong to.Having nearer sibship with paddy rice, wheat, is the monocotyledonous model plant of research.Because two fringe false bromegrass hereditary basiss are narrow, the germ plasm resource variety is low, therefore identifies relatively difficulty of two fringe false bromegrass powdery mildew genes through conventional molecule marker (RAPD, ISSR, SSR, AFLP etc.).The candidate's powdery mildew gene exploitation that is tested and appraised is divided into from functional mark (SNP, SCAR etc.) accordingly, can be used for the molecular marker assisted selection of disease-resistant gene fast, and accuracy is high.
3) initiative of multiresistance breeding material.Based on the functional molecular marker of the powdery mildew gene of new evaluation exploitation, combine the molecule marker of localized other disease-resistant genes, carry out the initiative of multiresistance breeding material, can shortening the breeding cycle, the raising breeding efficiency.
4) lay a good foundation for setting forth two fringe false bromegrass mildew-resistance molecular mechanisms.The evaluation of two fringe false bromegrass mildew-resistance genes; Gene silencing (virus induced gene silencing through transgenic technology, RNAi, virus induction; VIGS) molecular mechanism of research mildew-resistance such as technology provides genetic resources, helps setting forth fast the mechanism of action of two fringe false bromegrass mildew-resistances.
Description of drawings
The evaluation of Fig. 1 two fringe false bromegrass MLO genes;
That this figure shows is 12 MLO type gene identification results, and each gene all contains ' MLO ' conserved domain.
The evaluation of the Phylogenetic Relationships analysis of Fig. 2 plant MLO gene family and two fringe false bromegrass MLO type powdery mildew genes thereof;
Arabidopis thaliana is the model plant of plant science research; Grow in the tree at constructing system; The powdery mildew gene (PsMLO) of 15 MLO type genes of Arabidopis thaliana (wherein 3 genes are powdery mildew genes: AtMLO02, AtMLO06 and AtMLO12), tomato mildew-resistance gene (SlMLO), barley powdery mildew gene (HvMLO and HvMLO02) and pea is selected and is used for and two fringe false bromegrass MLO type gene cluster analyses.Identify 1 two fringe false bromegrass candidate's MLO type powdery mildew gene altogether.The gene of italic mark is exactly candidate's two fringe false bromegrass powdery mildew genes among the figure.
The compare of analysis of Fig. 3 two fringe false bromegrass MLO type powdery mildew genes;
1 two fringe false bromegrass powdery mildew gene and barley (HvMLO), tomato (SlMLO), pea (PsMLO), Arabidopis thaliana powdery mildew gene (AtMLO02; AtMLO06 and AtMLO12) compare, identify that powdery mildew infects the amino-acid residue and regional conservative type that plays an important role.TM1-TM7 representes 7 revolving die zones of two fringe false bromegrass MLO type powdery mildew genes among the figure; The black round dot representes that powdery mildew infects important amino-acid residue; CaMBD representes the calmodulin land; I and II represent powdery mildew is infected important amino acid region.
Embodiment
The evaluation of disease-resistant gene has important effect in research of crop disease-resistant theory of heredity and disease-resistant variety seed selection.Present method can Rapid identification go out two fringe false bromegrass powdery mildew genes.The practical implementation process is following:
1) collection and the evaluation of two fringe false bromegrass MLO type genes
In order to obtain the whole MLO type gene family member of two fringe false bromegrasses; We are at first with the MLO type gene of Arabidopis thaliana; The mildew-resistance MLO gene order of tomato, pea, capsicum, rose, capsicum, Root or stem of Littleleaf Indianmulberry makes up the HMM model, from two fringe false bromegrass genome sequences, receives rope MLO type gene; Secondly with the MLO gene order delivered in the Different Crop as target sequence (from DFCI DB: TC171015, TC267529, DFCI:TC327983, TC289653, TC312087, TC132500; TC133436, TC317623, TC317025, TC315947, TC325903, TC315944; TC315912, TC322759, TC322059, TC330654, TC282713, TC293173; TC281861, TC283253, TC283383, TC285032, TC290021, TC302716; TC283487, TC282866, TC283441, TC281428, TC285118, TC285090; From GenBank DB: AY967408, AF384145, AF384144; AY029312-AY029315, AY029317-AY029319, Z95352; AF369563-AF369565, AF369567, AF369569-AF369576; Z83834, Z95496, AY581255); Two fringe false bromegrass DBs (http://www.phytozome.net/search.php) are carried out the BLAST comparison, select the highest sequence of similarity to download, obtained 12 candidates' MLO type gene (Bradi1g36172 altogether; Bradi1g76500; Bradi2g25190; Bradi2g33517; Bradi2g57317; Bradi3g07150; Bradi3g32230; Bradi3g46070; Bradi4g08740; Bradi4g23887; Bradi5g11205; Bradi5g26435; ).
2) evaluation of two fringe false bromegrass MLO type gene families
In order further to verify these MLO gene accuracys, we carry out the evaluation of conserved domain " MLO " to these 12 MLO genes.Aminoacid sequence with each candidate's MLO type gene is a benchmark, on PFAM (http://pfam.sanger.ac.uk/) website, carries out the evaluation of ' MLO ' conserved domain, and concrete outcome is seen Fig. 1.
3) the Phylogenetic Relationships analysis of two fringe false bromegrass MLO type genes
In the research formerly, find that the dicotyledons powdery mildew gene aggregates into district's group; Therefore; Grow in the tree at constructing system; We have selected the powdery mildew gene and the two fringe false bromegrass MLO type gene cluster analysis cluster analyses together of 15 MLO type genes (wherein 3 genes are powdery mildew genes: AtMLO02, AtMLO06 and AtMLO12), tomato mildew-resistance gene, barley powdery mildew gene and the pea of model plant Arabidopis thaliana.Two fringe false bromegrass MLO type genes and other crop powdery mildew gene protein sequences are carried out the multisequencing couplet join (adopting Clustal X 1.83 softwares to carry out), and utilize Genedoc software (http://www.nrbsc.org/gfx/genedoc/index.html) to show that multisequencing joins the result who joins.The Clustal multisequencing is joined the result who joins output in MEGA 4.0 softwares, and (neighbor-joining NJ), utilizes the Bootstrapping method that these evolutionary trees are assessed to utilize this software to make up the adjacency tree respectively.The result finds in dicotyledons mildew-resistance gene district group, to have 1 two fringe false bromegrass MLO type powdery mildew gene (see figure 2).
4) comparison of two fringe false bromegrass MLO type disease-resistant genes
In the research of barley MLO type powdery mildew gene, found some important areas and single amino acids in the research in succession, they infect big wheat powdery mildew has irreplaceable effect.In order to identify in 1 the two fringe false bromegrass powdery mildew gene sequence; Whether these important areas and amino acid high conservative, and we have carried out compare of analysis to 3 mildew-resistance genes (AtMLO02, AtMLO6 and AtMLO12), tomato powdery mildew gene (SlMLO), the powdery mildew of pea gene (PsMLO) from Arabidopis thaliana.Stride the film district for 7 that find 1 powdery mildew gene of two fringe false bromegrasses and known MLO type powdery mildew gene, 30 important amino acid, 1 calmodulin land (CaMBD) and two important zone (I and II) high conservatives (Fig. 3).
Claims (4)
1. two fringe false bromegrass mildew-resistance genes is characterized in that being selected from following 1 gene:
Gene numbering: Bradi1g76500
Aminoacid sequence:
MAGPAVGRELPQTPTWAVAVVCLVMILLSLALEHALHKLGHWFQKRQKKAVAEALEKIKAELMLMGFISLLLTVGQTPISKICISKEAGSVMLPCKLSVAAEDADDEGKDNGRRRLLWFQEEIHRRFLAAAPGVDPCASQGKVALMSASSMHQLHIFIFVLAVFHVFYSVTTMALGRLKMKKWKKWESETTSLEYQFANDPSRFRFTHQTSFVKRHMGLSSTPGVRWIVAFFRQFFGSVTKVDYLTMRQGFINAHLSQNSKFDFHKYIKRSLEDDFKVVVGISLPLWFVAVLTLFLDINGIYTLIWISFVPFVILLLVGTKLEIVIMEMAQEIQDRASVIKGAPVVEPSNKFFWFKRPDWVLFLIHLTLFQNAFQMAHFVWALFTPGLKKCYHQNMGLSIMKVVVGVALQVLCSYITFPLYALVTQMGSNMKRAIFDEQTAKALTNWRNTAREKKKTRDADAFMAQMIGDATPSQGSSPVHLLHKNRMRSEDPQSIPTSPRAEHEAMDMYPVAQVVHTSHPHPPHRLDPSDRRRSASSSALDTDIASADFSFSMQR
Nucleotide sequence:
ATGGCGGGGCCGGCGGTAGGGCGGGAGCTGCCGCAGACGCCGACGTGGGCGGTGGCGGTCGTCTGCCTCGTCATGATACTCCTCTCCCTCGCCTTGGAGCACGCGCTCCACAAGCTCGGCCATTGGTTCCAGAAGCGGCAGAAGAAGGCCGTGGCCGAGGCGCTCGAGAAGATCAAAGCAGAGCTGATGCTGATGGGTTTCATTTCCCTGCTCCTCACCGTGGGGCAAACGCCAATCTCCAAGATATGCATCTCCAAGGAGGCCGGCAGCGTCATGCTACCGTGCAAGCTGTCAGTGGCAGCCGAGGACGCCGACGACGAAGGCAAGGACAACGGCCGCCGGAGGCTACTCTGGTTCCAGGAAGAAATCCACCGTCGGTTCTTGGCCGCCGCGCCCGGAGTTGACCCCTGTGCGAGCCAGGGCAAGGTGGCGCTGATGTCTGCAAGCAGCATGCACCAGCTGCACATATTCATCTTCGTGCTCGCCGTCTTCCATGTCTTCTACAGCGTCACCACCATGGCTCTAGGGCGTCTCAAGATGAAGAAATGGAAGAAATGGGAGTCGGAGACAACCTCACTGGAGTATCAGTTCGCAAATGATCCTTCGCGGTTCCGTTTCACGCACCAGACGTCCTTCGTGAAGCGGCACATGGGCCTCTCCAGCACTCCTGGCGTCAGATGGATCGTGGCGTTCTTCAGGCAGTTCTTCGGGTCAGTCACCAAGGTGGACTACCTGACCATGCGCCAAGGCTTCATCAATGCGCATTTGTCGCAGAACAGCAAGTTCGACTTCCACAAATACATCAAGAGGTCACTCGAGGACGACTTCAAAGTAGTCGTCGGTATCAGCCTCCCGCTGTGGTTCGTGGCGGTTCTCACGCTCTTCCTTGATATTAACGGGATCTACACGCTCATCTGGATCTCTTTCGTCCCTTTCGTCATCCTGTTGCTGGTTGGAACCAAGCTAGAGATTGTGATCATGGAGATGGCCCAGGAGATCCAGGATCGGGCAAGCGTCATCAAGGGGGCTCCTGTCGTTGAACCAAGCAACAAGTTCTTCTGGTTCAAACGGCCTGATTGGGTCCTGTTCCTCATACATTTGACACTGTTCCAGAACGCGTTTCAGATGGCGCATTTCGTCTGGGCACTGTTCACACCCGGTTTGAAGAAATGCTACCATCAAAACATGGGACTGAGCATCATGAAGGTCGTGGTGGGGGTAGCTCTTCAGGTTCTGTGCAGCTACATCACCTTCCCACTCTATGCACTAGTCACACAGATGGGCTCAAACATGAAGAGGGCCATCTTCGATGAGCAGACGGCCAAGGCACTGACGAATTGGAGGAACACAGCAAGGGAGAAGAAGAAGACCCGGGATGCAGACGCATTCATGGCGCAGATGATCGGTGACGCAACACCAAGCCAAGGCTCATCGCCGGTGCATCTGCTCCACAAGAACAGGATGCGGTCAGAAGATCCCCAAAGCATACCAACCTCGCCAAGGGCCGAGCACGAGGCTATGGACATGTATCCGGTTGCGCAGGTTGTGCACACATCGCATCCACATCCACCCCACAGACTAGACCCTTCTGACAGGAGGAGGTCCGCCTCATCGTCAGCCCTTGACACTGATATAGCCAGTGCTGATTTTTCCTTCAGCATGCAACGGTGA。
2. the application of the said Rapid identification two fringe false bromegrass powdery mildew genes of claim 1 comprises:
1) to the transfer of gramineous crop mildew-resistance gene.
3.2) to the transfer breeding practice of gramineous crop mildew-resistance gene.
4.3) mildew-resistance fundamental research.
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| CN104561254A (en) * | 2013-10-28 | 2015-04-29 | 常熟市董浜镇里睦蔬菜专业合作社 | Rapid identification method of powdery mildew-resistant genes of cabbage |
| CN104561032A (en) * | 2013-10-28 | 2015-04-29 | 常熟市董浜镇里睦蔬菜专业合作社 | Quick identification of powdery mildew resistant gene for barley |
| CN104561253A (en) * | 2013-10-28 | 2015-04-29 | 南农大(常熟)新农村发展研究院有限公司 | Rapid identification of corn powdery mildew resistant genes |
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| CN104593480A (en) * | 2013-10-30 | 2015-05-06 | 江苏省常熟现代农业产业园区发展有限公司 | Application of comparative genomics to rapid identification of phaseolus vulgaris mildew resistance locus o gene |
| CN104593481A (en) * | 2013-10-30 | 2015-05-06 | 江苏省常熟现代农业产业园区发展有限公司 | Rapid identification of soybean anti-powdery mildew gene by using candidate gene strategy |
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| CN104561034A (en) * | 2013-10-28 | 2015-04-29 | 常熟市杜桥稻米专业合作社 | Rapid identification method for powdery mildew resistant gene of foxtail millet |
| CN104561024A (en) * | 2013-10-28 | 2015-04-29 | 南农大(常熟)新农村发展研究院有限公司 | Rapid identification of MLO (Mycoplasma Like Organism) powdery mildew resistant genes of melon |
| CN104561033A (en) * | 2013-10-28 | 2015-04-29 | 常熟市杜桥稻米专业合作社 | Rapid identification on wheat MLO type powdery mildew gene |
| CN104593480A (en) * | 2013-10-30 | 2015-05-06 | 江苏省常熟现代农业产业园区发展有限公司 | Application of comparative genomics to rapid identification of phaseolus vulgaris mildew resistance locus o gene |
| CN104593481A (en) * | 2013-10-30 | 2015-05-06 | 江苏省常熟现代农业产业园区发展有限公司 | Rapid identification of soybean anti-powdery mildew gene by using candidate gene strategy |
| CN108026540A (en) * | 2015-07-17 | 2018-05-11 | 中国科学院遗传与发育生物学研究所 | The wheat plant of mildew-resistance |
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