CN104611385A - 一种使用同一菌株cgmcc 1593发酵耦合制备乳酸和丁二酸的方法 - Google Patents
一种使用同一菌株cgmcc 1593发酵耦合制备乳酸和丁二酸的方法 Download PDFInfo
- Publication number
- CN104611385A CN104611385A CN201510100512.XA CN201510100512A CN104611385A CN 104611385 A CN104611385 A CN 104611385A CN 201510100512 A CN201510100512 A CN 201510100512A CN 104611385 A CN104611385 A CN 104611385A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- fermentation system
- prepares
- succinic acid
- lactic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 67
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 title claims abstract description 50
- 230000004151 fermentation Effects 0.000 title claims abstract description 48
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 30
- 239000004310 lactic acid Substances 0.000 title claims abstract description 22
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 22
- 239000001384 succinic acid Substances 0.000 title claims abstract description 18
- 230000008878 coupling Effects 0.000 title claims abstract description 5
- 238000010168 coupling process Methods 0.000 title claims abstract description 5
- 238000005859 coupling reaction Methods 0.000 title claims abstract description 5
- 238000010564 aerobic fermentation Methods 0.000 claims abstract description 27
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 19
- 230000001580 bacterial effect Effects 0.000 claims abstract description 14
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims abstract description 9
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000005695 Ammonium acetate Substances 0.000 claims abstract description 8
- 235000019257 ammonium acetate Nutrition 0.000 claims abstract description 8
- 229940043376 ammonium acetate Drugs 0.000 claims abstract description 8
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims abstract description 7
- 239000001632 sodium acetate Substances 0.000 claims abstract description 7
- 235000017281 sodium acetate Nutrition 0.000 claims abstract description 7
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 5
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- 238000011218 seed culture Methods 0.000 claims description 18
- 229960005137 succinic acid Drugs 0.000 claims description 17
- 230000008569 process Effects 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 229910019142 PO4 Inorganic materials 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 12
- 235000005822 corn Nutrition 0.000 claims description 12
- 239000008367 deionised water Substances 0.000 claims description 12
- 229910021641 deionized water Inorganic materials 0.000 claims description 12
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 12
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 12
- 235000019800 disodium phosphate Nutrition 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 12
- 239000010452 phosphate Substances 0.000 claims description 12
- 229910052700 potassium Inorganic materials 0.000 claims description 12
- 239000011591 potassium Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 11
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 claims description 10
- 241000948980 Actinobacillus succinogenes Species 0.000 claims description 8
- AIMMVWOEOZMVMS-UHFFFAOYSA-N cyclopropanecarboxamide Chemical compound NC(=O)C1CC1 AIMMVWOEOZMVMS-UHFFFAOYSA-N 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 230000001186 cumulative effect Effects 0.000 claims description 4
- 230000009897 systematic effect Effects 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 2
- 238000007254 oxidation reaction Methods 0.000 claims 2
- 229910052708 sodium Inorganic materials 0.000 claims 2
- 239000011734 sodium Substances 0.000 claims 2
- 239000007789 gas Substances 0.000 abstract description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 3
- 229910052799 carbon Inorganic materials 0.000 abstract description 3
- 229910052760 oxygen Inorganic materials 0.000 abstract description 3
- 239000001301 oxygen Substances 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 230000002159 abnormal effect Effects 0.000 abstract description 2
- 238000011109 contamination Methods 0.000 abstract 1
- 239000011261 inert gas Substances 0.000 abstract 1
- 230000002503 metabolic effect Effects 0.000 abstract 1
- 235000010265 sodium sulphite Nutrition 0.000 abstract 1
- 235000011089 carbon dioxide Nutrition 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 239000002253 acid Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 238000011169 microbiological contamination Methods 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- -1 poly(lactic acid) Polymers 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- OVYQSRKFHNKIBM-UHFFFAOYSA-N butanedioic acid Chemical compound OC(=O)CCC(O)=O.OC(=O)CCC(O)=O OVYQSRKFHNKIBM-UHFFFAOYSA-N 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 229920006238 degradable plastic Polymers 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000003204 tranquilizing agent Substances 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510100512.XA CN104611385B (zh) | 2015-03-09 | 2015-03-09 | 一种使用同一菌株cgmcc 1593发酵耦合制备乳酸和丁二酸的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510100512.XA CN104611385B (zh) | 2015-03-09 | 2015-03-09 | 一种使用同一菌株cgmcc 1593发酵耦合制备乳酸和丁二酸的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104611385A true CN104611385A (zh) | 2015-05-13 |
CN104611385B CN104611385B (zh) | 2017-10-31 |
Family
ID=53146035
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510100512.XA Active CN104611385B (zh) | 2015-03-09 | 2015-03-09 | 一种使用同一菌株cgmcc 1593发酵耦合制备乳酸和丁二酸的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104611385B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114277065A (zh) * | 2021-12-30 | 2022-04-05 | 万华化学集团股份有限公司 | 一种混合发酵联产乳酸和丁二酸的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101250561A (zh) * | 2008-04-15 | 2008-08-27 | 中国科学院微生物研究所 | 一种发酵生产丁醇和丁二酸的方法 |
CN101613722A (zh) * | 2009-07-31 | 2009-12-30 | 安徽丰原发酵技术工程研究有限公司 | 一种利用纤维素类原料发酵生产乙醇和丁二酸的方法 |
-
2015
- 2015-03-09 CN CN201510100512.XA patent/CN104611385B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101250561A (zh) * | 2008-04-15 | 2008-08-27 | 中国科学院微生物研究所 | 一种发酵生产丁醇和丁二酸的方法 |
CN101613722A (zh) * | 2009-07-31 | 2009-12-30 | 安徽丰原发酵技术工程研究有限公司 | 一种利用纤维素类原料发酵生产乙醇和丁二酸的方法 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114277065A (zh) * | 2021-12-30 | 2022-04-05 | 万华化学集团股份有限公司 | 一种混合发酵联产乳酸和丁二酸的方法 |
CN114277065B (zh) * | 2021-12-30 | 2023-10-13 | 万华化学集团股份有限公司 | 一种混合发酵联产乳酸和丁二酸的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN104611385B (zh) | 2017-10-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102827775B (zh) | 微藻培养固定微生物发酵尾气co2补充发酵原料的方法 | |
CN101215584B (zh) | 农作物秸秆生物转化制备琥珀酸工艺 | |
CN104371960B (zh) | 复合菌剂及所采用的复合微生物的连续发酵法 | |
CN102643770B (zh) | 利用合成培养基纯厌氧生长产丁二酸的大肠杆菌及其应用 | |
CN101709263B (zh) | 一种恒化器连续培养装置 | |
CN102199570B (zh) | 构建基因工程菌以增强微生物发酵甘油产1,3-丙二醇的方法 | |
CN103952447B (zh) | 一种利用厌氧条件下发酵生产丁二酸的方法 | |
CN102093990B (zh) | 微生物发酵生产低温淀粉酶的方法 | |
CN106119289A (zh) | 一种复合菌群协同降解秸秆纤维素和发酵产氢的联合预处理方法 | |
CN102286600A (zh) | 一种利用木薯渣同时发酵生产乙醇和氢气的方法 | |
CN105779328A (zh) | 一种热纤梭菌的培养方法 | |
CN111334532A (zh) | 一种丁酸连续发酵的方法 | |
CN102051386B (zh) | 间歇式回流细胞高生产率发酵生产有机酸的方法 | |
CN105177058B (zh) | 一种调节厌氧食气微生物发酵产物中酸醇比例的方法 | |
CN104611385A (zh) | 一种使用同一菌株cgmcc 1593发酵耦合制备乳酸和丁二酸的方法 | |
CN102925495A (zh) | 一种利用糖质原料连续发酵生产丁醇的方法 | |
CN100497611C (zh) | 一种发酵法制备核酸酶p1的方法 | |
CN101768610A (zh) | 一种高效培养乳酸菌和生产乳酸的方法 | |
CN104357425A (zh) | 一种新型α-半乳糖苷酶NGAL及其基因和应用 | |
CN102296093A (zh) | 一种氧化还原电位调控厌氧发酵生产丁醇的方法 | |
CN103320367A (zh) | 一株厌氧利用合成培养基高产丁二酸大肠杆菌的筛选及其应用 | |
CN101643753B (zh) | 一种木糖醇的制备方法 | |
CN1216145C (zh) | 一种嗜热乳酸杆菌以及采用该菌种生产l-乳酸的方法 | |
CN104630188A (zh) | 海洋微生物发酵生产低温葡甘聚糖酶的方法 | |
CN112608839B (zh) | 一种基于静电场发酵罐促进酵母生长的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20220630 Address after: 443000 room 1018, No. 57-5, development avenue, Yichang District, China (Hubei) free trade zone, Yichang City, Hubei Province Patentee after: Hubei Guoxin Juzhi New Material Technology Co.,Ltd. Address before: 214000 Wuxi Baofeng Petrochemical Equipment General Factory, Sunshine Industrial Park, Donggang Town, Xishan District, Wuxi City, Jiangsu Province Patentee before: Lv Tao |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20221229 Address after: 201512 Building 2, No. 688, Qiushi Road, Jinshanwei Town, Jinshan District, Shanghai (Economic Community of Jinshan No. 2 Industrial Zone) Patentee after: Shanghai Chengxien Biochemical Technology Co.,Ltd. Address before: 443000 room 1018, No. 57-5, development avenue, Yichang District, China (Hubei) free trade zone, Yichang City, Hubei Province Patentee before: Hubei Guoxin Juzhi New Material Technology Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231207 Address after: Room 378, Building B, Building 2, No. 399 Danfeng Road, Xixing Street, Binjiang District, China (Zhejiang) Pilot Free Trade Zone, Hangzhou City, Zhejiang Province, 310053 Patentee after: Hangzhou Shien Technology Co.,Ltd. Address before: 201512 Building 2, No. 688, Qiushi Road, Jinshanwei Town, Jinshan District, Shanghai (Economic Community of Jinshan No. 2 Industrial Zone) Patentee before: Shanghai Chengxien Biochemical Technology Co.,Ltd. |