CN104605051A

CN104605051A - Double hemp-folium cortex eucommiae tea and preparing method thereof

Info

Publication number: CN104605051A
Application number: CN201510059822.1A
Authority: CN
Inventors: 杨槐; 梁斌; 张丽艳; 唐靖雯; 潘梅; 王传芳; 彭再刚
Original assignee: GUIZHOU WARMEN PHARMACEUTICAL CO Ltd
Current assignee: GUIZHOU WARMEN PHARMACEUTICAL CO Ltd
Priority date: 2015-02-05
Filing date: 2015-02-05
Publication date: 2015-05-13
Anticipated expiration: 2035-02-05
Also published as: CN104605051B

Abstract

The invention relates to double hemp-folium cortex eucommiae tea and a preparing method thereof. Specifically, the invention relates to a health product with hypotensive activity and prepared from folium apocyni veneti, folium cortex eucommiae, rhizoma gastrodiae, flos sophorae, flos chrysanthemi and green tea as raw materials. More particularly, the invention also relates to a health product with an auxiliary blood pressure reduction function and prepared from the folium apocyni veneti, the folium cortex eucommiae, the rhizoma gastrodiae, the flos sophorae, the flos chrysanthemi and the green tea as raw materials. The invention also relates to a method for preparing a composition. The composition disclosed by the invention has an excellent auxiliary blood pressure reduction function.

Description

Two numb tea containing leaves of Eucommia ulmoides and method for making

Technical field

The invention belongs to health-oriented products field, relate to a kind of health-oriented products comprising hypotensive activity material, particularly relate to a kind of health-oriented products with hypotensive activity made for raw material with Folium Apocyni Veneti, folium cortex eucommiae, rhizoma Gastrodiae, sophora flower, chrysanthemum, green tea.More especially relate to a kind of health product of the auxiliary function for lowering blood pressure made for raw material with Folium Apocyni Veneti, folium cortex eucommiae, rhizoma Gastrodiae, sophora flower, chrysanthemum, green tea.

Background technology

Hypertension is common angiocardiopathy, and easily cause the complication of the internal organs such as the heart, brain, kidney, the health of the mankind in serious harm, and case fatality rate and disability rate are all very high.The morbidity of high blood pressure increases with the age and increases, and it is reported that the Prevalence of Hypertension of the whole nation in 1991 >=60 years old crowd is 40.4%, and the illness rate of over-65s crowd has exceeded 50% at present.When China progressively enters aging society, love the elderly is healthy, prevents and treats hypertension, has become current government and Chinese scholars question of common concern.

According to the investigation of 2002, China's adult hypertension illness rate was 18.8%, and there is hyperpietic 1.6 hundred million in the whole nation, and within 1991, compared illness rate and rose 31%.According to another statistics, China aging populations is 9%, nearly 5,000 ten thousand people of Aged Patients With Essential Hypertension, every year because hypertension causes the heart, brain, kidney trouble died, and 1,200 ten thousand people nearly.And according to the data display that the United Nations issues, will account for 20% of country's total population to Aged in China population in 2025, to the year two thousand fifty, elderly population accounting even may more than 40%.What form powerful contrast with high incidence is low awareness, low treatment rate and low control rate.Have data to show, population of China hyperpiesia awareness is 30.2%, and treatment rate is 24.7%, and control rate is 6.1%.

Zhang Puhong equals to investigate situations such as Beijing area adult's epidemic features of hypertensions in September, 2005 to October, respondent is the adult of 18 ~ 92 years old, totally 16658 people, wherein the male sex 6605 people, women 10053 people, the overall illness rate of result hypertension is 29.1%, wherein 18 ~ 29 years old group 7.0%, 30 ~ 39 years old group 18.3%, 40 ~ 49 years old groups 35.3%, 50 ~ 59 years old groups, 50.6%, 60 ~ 69 years old group, 72.3%, 70 ~ 92 years old group 77.8%.No matter sex, residence, hypertensive illness rate all significantly rises with the growth at age.The illness rate of middle-aging male is consistent with the age bracket residing for it, and within such as 40 ~ 49 years old, to organize hypertensive illness rate be 44.4%, 50 ~ 59 years old group is 52.3%.Women before 50 years old hypertensive illness rate significantly lower than the male sex, 50 years old afterwards significantly rising even exceed the male sex.In the hyperpietic of place, hypertension awareness is 49.3%, and overall control rate is 10.6%.

Yin Chuangui equals to have investigated part rural area, Yishui, Shandong Province high blood pressure Epidemiology in January, 2008.Randomly draw resident 4805 people of more than 18 years old of three small towns, Yishui, adopt unified way of questionnaires, statistical analysis is carried out to gained data.Result: the hypertensive illness rate of population was 24.9% in more than 18 years old; No matter masculinity and femininity, Prevalence of Hypertension all raises along with the increase at age; Hypertensive awareness, treatment rate, control rate are respectively 19.6%, 12.7%, 4.8%; Mainly high salt diet, the smoking of hypertensive hazards, drink, hyperlipidemia, diabetes and family history of hypertension etc.

Xun's equality has carried out " Among Rural Adult Groups in Liaoning Province hypertension, cerebral apoplexy low cost pai n nursing are studied " in 2004 ~ 2005 years at Liaoning Province's Fuxin City, by the method for stratified cluster random sampling, 8 small towns, 84 village >=60 year old inhabitant 10065 people are investigated, wherein male 5092 people, female 4973 people, result Prevalence of Hypertension is 60.2%, the wherein male sex 57.9%, women 62.6%, the patient of 35% knows oneself to suffer from hypertension, the people of 28.7% takes depressor, but only has the patients' blood of 0.6% to be controlled.

Left pellet equals to investigate the mid-aged population prevalence of hypertension that cities and counties of 12, Hubei Province are greater than 35 years old the age in September, 2003 to October, investigate 2811 people altogether, reclaim effective questionnaire 2709 parts, wherein male 1536 people, female 1173 people, hypertension total prevalence rate is 42.3%, wherein man 44.8%, and female 39.8%.

Song Xiangxin equals more than the 18 years old inhabitant of in July, 2006 to September to Gong25Ge village of 9 townshiies, In Turfan In Xinjiang and has carried out hypertensive epidemiology survey, investigate 10185 people altogether, the wherein Uygur nationality 9217 people, Han nationality 674 people, other national 294 people, the male sex 4239 people, women 5946 people, in year survey group's mean age (56.41 ± 10.42), the overall Prevalence of Hypertension of result is 25.3%, wherein the male sex 27.3%, women 23.9%, Uygur nationality's illness rate is 24.4%, and Han nationality is 35.6%, and other nationalitys are 33.0%.The crowd's Prevalence of Hypertension being less than 30 years old only has 5.6%, crowd's Prevalence of Hypertension 11.9% of 30 ~ 39 years old, crowd's Prevalence of Hypertension crowd's Prevalence of Hypertension 35.8% of 25.5%, 50 ~ 59 years old of 40 ~ 49 years old, is greater than crowd's Prevalence of Hypertension 43.9% of 60 years old.

As can be seen from above data, China's various places Prevalence of Hypertension is all higher, wherein the elderly group of people at high risk especially, and the market demand improving hypertension is very big.Although treat hypertension at present, Western medicine is first-selected, but due to high blood pressure be chronic disease, need take medicine even throughout one's life for a long time, and general Western medicine step-down is mainly simple starts with from aspects such as hemangiectasis, diuresis, enzyme retardances, although rapid-action, not comprehensively regulating, only simple step-down, then blood pressure of taking medicine declines, drug withdrawal then blood pressure raises, and cures the symptoms, not the disease, and the side effect that long-term taking produces is very large to people's harm.Have the saying of " Diet cures more than the doctors " in motherland's medical science, along with the raising of people's health care consciousness, increasing people recognizes the importance of tonic.By adjustment diet, take effective health product to the method improving health also just accept by increasing people.Therefore the health product of auxiliary function for lowering blood pressure is subject to the common concern of people, there is the wide market demand.

Therefore, this area still needs the health-oriented products with hypotensive activity, particularly needs the health product with auxiliary function for lowering blood pressure, and expects that this product has the excellent properties of at least one aspect.

Summary of the invention

The object of ability invention is to provide a kind of health-oriented products with hypotensive activity, particularly provides a kind of health product with auxiliary function for lowering blood pressure, and expects that this product has the excellent properties of at least one aspect.Have been surprisingly found that, with Folium Apocyni Veneti, folium cortex eucommiae, rhizoma Gastrodiae, sophora flower, chrysanthemum, green tea is raw material, through pulverizing, screening, mixing, dry, total mixed, inner packing, external packing, the health product of what the main technique such as sterilizing were made have auxiliary function for lowering blood pressure, can be described as two numb tea containing leaves of Eucommia ulmoides or the two numb tea containing leaves of Eucommia ulmoides of your health board old in the present invention, its formula, meet national relevant laws and regulations requirement, there is science, novelty and creativeness, its mature production technology, stable, be easy to quality control, dosage form selection bag tea agent, adopt composite film packaging, attractive in appearance, be easy to take, volume is little, easy to carry, the peaceful property test of toxicity shows edible safety, can eat for a long time, animal and human's body function test shows the function with aided blood pressure-lowering.Result shows, the two numb tea containing leaves of Eucommia ulmoides of your health board old of the present invention has higher scientific level.Therefore the present invention is accomplished.

For this reason, first aspect present invention provides a kind of composition that can be described as two numb tea containing leaves of Eucommia ulmoides or the two numb tea containing leaves of Eucommia ulmoides of your health board old, and it is prepared by following material: rhizoma Gastrodiae, Folium Apocyni Veneti, folium cortex eucommiae, sophora flower, chrysanthemum, green tea.

The composition of arbitrary embodiment according to a first aspect of the present invention, it is prepared by following material:

In the present invention, use rhizoma Gastrodiae as Pharmacopoeia of the People's Republic of China version in 2010 54 pages record.

In the present invention, use Folium Apocyni Veneti as Pharmacopoeia of the People's Republic of China version in 2010 196 pages record.

In the present invention, use folium cortex eucommiae as Pharmacopoeia of the People's Republic of China version in 2010 154 pages record.

In the present invention, use sophora flower as Pharmacopoeia of the People's Republic of China version in 2010 333 pages record.

In the present invention, the chrysanthemum of use as Pharmacopoeia of the People's Republic of China version in 2010 292 pages record, and containing 3,5-O-DCQ (C25H24O12) in this medicinal material.

In the present invention, the green tea of use well known to a person skilled in the art, such as, under version " Chinese medicine voluminous dictionary " (Shanghai science tech publishing house) contained " tealeaves " item in 1986 described in.

The composition of arbitrary embodiment according to a first aspect of the present invention, it is the form in tea in bag.

The composition of arbitrary embodiment according to a first aspect of the present invention, it is the form in tea in bag, and every bag of weight comprising rhizoma Gastrodiae is 0.1-0.3g.

The composition of arbitrary embodiment according to a first aspect of the present invention, it is the form in tea in bag, and every bag of weight comprising rhizoma Gastrodiae is 0.15-0.25g.

The composition of arbitrary embodiment according to a first aspect of the present invention, it is the form in tea in bag, and every bag of weight comprising rhizoma Gastrodiae is 0.2g.

The composition of arbitrary embodiment according to a first aspect of the present invention, it is prepared by the method comprised the following steps:

(1) Folium Apocyni Veneti, folium cortex eucommiae, sophora flower, chrysanthemum rinse by purified water respectively, dry 6-8h for 60 DEG C, for subsequent use;

(2) rhizoma Gastrodiae purified water is rinsed, and dries 6-8h, breaks into pieces for 60 DEG C, for subsequent use;

(3) take green tea by formula rate to pulverize together with chrysanthemum, with 24 orders and the screening of 65 eye mesh screens, get meal;

(4) by Folium Apocyni Veneti, folium cortex eucommiae, rhizoma Gastrodiae, the sophora flower through pre-treatment, pulverize respectively, with 24 orders and the screening of 65 eye mesh screens, get each meal, mix with step (3) gained meal, 60 DEG C of dry 3h, total mixed 30min, carry out composite film packaging with packaging machine of tea bag, 6kGy irradiation sterilization, to obtain final product.

Have been surprisingly found that, make green tea together with chrysanthemum after pulverization process, when carrying out point packing with packaging machine of tea bag in step (4), in early stage, mid-term of point packaging process, the material in latter stage, the content of 3,5-O-DCQ (C25H24O12) has goodish uniformity; And if by chrysanthemum and other material or all together with material during pulverization process; then to cause in the early stage material of point packaging process total 3; the content of 5-O-DCQ is with mixed material is suitable eventually; and the mid-term of point packaging process is in material 3; the content of 5-O-DCQ is higher (relative to mixing material eventually); latter stage of point packaging process is the content of 3,5-O-DCQs (relative to mixed material eventually) on the low side in material.

Further, second aspect present invention provides a kind of method preparing the composition that can be described as two numb tea containing leaves of Eucommia ulmoides or the two numb tea containing leaves of Eucommia ulmoides of your health board old, and said composition is prepared by following material: rhizoma Gastrodiae, Folium Apocyni Veneti, folium cortex eucommiae, sophora flower, chrysanthemum, green tea; The method comprises the following steps:

The method of arbitrary embodiment according to a second aspect of the present invention, wherein said composition is prepared by following material:

The method of arbitrary embodiment according to a second aspect of the present invention, wherein said composition is the form in tea in bag.

The method of arbitrary embodiment according to a second aspect of the present invention, wherein said composition is the form in tea in bag, and every bag of weight comprising rhizoma Gastrodiae is 0.1-0.3g.

The method of arbitrary embodiment according to a second aspect of the present invention, wherein said composition is the form in tea in bag, and every bag of weight comprising rhizoma Gastrodiae is 0.15-0.25g.

The method of arbitrary embodiment according to a second aspect of the present invention, wherein said composition is the form in tea in bag, and every bag of weight comprising rhizoma Gastrodiae is 0.2g.

Arbitrary technical characteristic that arbitrary embodiment of either side of the present invention or this either side has is suitable for arbitrary embodiment of other arbitrary embodiment or other either side equally, as long as they can not be conflicting, certainly at where applicable each other, necessary words can be done suitably to modify to individual features.Be further described with feature to various aspects of the present invention below.

The health food with auxiliary function for lowering blood pressure having obtained at present national interested regulatory authorities authentication code has: grand year board red sage root gastrodia capsule, adopted and modest board founds prosperous capsule, long strong aircraft carrier board delightful harmony capsule, middle section board Sai Nuoping capsule, seedling strange board seedling health tea, century health board Ge Sheng capsule, the refined rich Yiganning capsule of huge strength board, purple cotton board pseudo-ginseng radix puerariae capsule, the sharp board Eucommia Tea of antelope, red damp board Pueraria lobota lamb's-quarters capsule, Yao Zhi garden board Shu Le sheet, precious sesame source board Long Gexin snowflake, the clear rhythm granule of hundred boards difficult to understand, the refined relieving capsule of high official position mountain board, the safe tea of green mountain nine board bifurcation, original classical prescription board bark of eucommia radix puerariae capsule, its secondary tea of wide beneficial hall board pool, the star capsule of the Star Pueraria lobota of Pueraria lobota, the prosperous board gastrodia elata-cortex capsule in port, the strange tenth of the twelve Earthly Branches every board quassia capsule, many Bang Yakang board many Bang Yakang sheet, capsule hidden by snow Wang Shan board, glad capsule nourished by precious feelings board, clear outstanding clear board red colouring agent for food, also used as a Chinese medicine flavones sheet, snow Yiganning capsule hidden by good fortune source board, interwined dragon board sky sieve soothing soft capsule, gold Na board pseudo-ginseng chrysanthemum tea, east is carried out board filamentary silver and is protected capsule, east is carried out board filamentary silver and is protected sheet etc.Formulation mostly is capsule, also has medicinal tea, tablet and granule.Wherein the prosperous board gastrodia elata-cortex capsule in port is made up of rhizoma Gastrodiae and the bark of eucommia, with prescription of the present invention relatively.

Formula material of the present invention selects the recipe requirements all meeting health food, is be instruct with theory of traditional Chinese medical science, carries out prescription in conjunction with modern medical theory, its feature is that each taste raw material all has independent antihypertensive effect, usually compatibility uses again clinically, and to strengthen antihypertensive effect, therefore effect is stronger; In formula, most of raw material is leaf class, flower class Chinese medicine, among the peoplely often make step-down tea-drinking, add that there is the tea culture of thousands of years in China, Chinese medicine and green tea compatibility are made bag tea agent, " take medicine " while having tea, the tension of " taking medicine " can not be caused to people; As hypertensive group of people at high risk, the elderly has dysphagia more, and compare tablet, capsule, medicinal tea is more suitable for the elderly and takes.And the feature of " quantitatively, health, convenience " compared conventional tea and have by tea in bag, represent the development trend of Tea Industry.In sum, compare with other similar health-care food, this product has certain comprehensive advantage, is the health food of safe, effective, the taking convenience aiming at suitable population's design.

First recipe determination of the present invention sets about from the health care of aided blood pressure-lowering.

Traditional Chinese medicine is thought, high blood pressure belongs to the category such as " dizzy ", " headache ", " liver wind ".High blood pressure is divided into overabundant liver-fire, hyperactivity of yang due to yin deficiency by the Ministry of Public Health in 1993 " new Chinese medicine treats hypertensive guideline of clinical investigations ", yin-yang deficiency and phlegm is wet stops up 4 types such as Sheng.Clinical epidemiology observes display, and liver-yang hyperactivity, hyperactivity of yang due to yin deficiency, the deficiency of liver-yin and kidney-yin are liver wind to be disturbed the main card marquis into high blood pressure ^[9].The common headache of symptom is dizzy, soreness and weakness of waist and knees, dizziness and tinnitus, and eyes is dim-sighted, palpitation and insomnia, dysphoria, extremity numbness etc.Many because of the deficiency of the kidney yin, water files to nourish wood, the liver losing its adequate flourishment, and the moon does not tie up sun, liver-yang hyperactivity, liver wind agitation, therefore see that headache is dizzy, dizzy; Kidney deficiency and liver, therefore see soreness and weakness of waist and knees, tinnitus; Liver opening at eye, the deficiency of liver-yin and kidney-yin, therefore see that eyes is dim-sighted; Liver governing tendons arteries and veins, the liver failing to be nourished, therefore extremity numbness; Kidney water can not on help in the heart, the heart is become homeless foster, and liver-yang is had a surplus again, and the heart is disturbed in heat-transmission, therefore sees palpitation and insomnia, dysphoria.Control and should tonify the liver and kidney, clearing heat and relieving fidgetness, suppressing liver-YANG, dispel the wind surely dizzy.The Chinese medicine that in current clinical step-down prescription, frequency of utilization is higher has the root of bidentate achyranthes, yncaria stem with hooks, Ligusticum wallichii, selfheal, the dried rhizome of rehmannia, chrysanthemum, the shell of seaear, rhizoma Gastrodiae, rhizoma alismatis, the root of herbaceous peony etc., and bibliographical information has obvious step-down and the medicine that improves complication has the (Qiu Zhichun such as the root of bidentate achyranthes, yncaria stem with hooks, rhizoma Gastrodiae, the bark of eucommia, parasitic loranthus, the red sage root, moutan bark, Ligusticum wallichii, the root of kudzu vine, safflower, Stephania tetrandra, rhizoma alismatis, earthworm, permitted family's red horse. my institute's high blood pressure prescriptions of traditional Chinese medicine statistical analysis. traditional Chinese medicine Leader .2005,11 (5): 51-52).

Folium Apocyni Veneti among the people as medicinal existing history for a long time, once mention the masses in the bluish dogbane investigation report of Shandong and have this experience " drunk tea that tea stalk (i.e. Folium Apocyni Veneti) steeps can refrigerantly reduce internal heat gas ", " weather is too warm when cutting wheat; drunk tea stalk tea and can prevent dizziness ", a word like this that also spreads among the people: " hypertension is not fearful, 1 year three jin of bluish dogbane." China begins one's study from the sixties in 20th century hypotensive effect of Folium Apocyni Veneti; see the drop test being reported as the Chinese Academy of Sciences (Shanghai) institute of materia medica in 1969 and carrying out the earliest; test proves that the model of Folium Apocyni Veneti to renal hypertension dog or cat has hypotensive effect; can slow down to the rhythm of the heart, human peripheral blood pipe is obviously expanded.Since 1977, Folium Apocyni Veneti records among each version Pharmacopoeia of the People's Republic of China, is the Chinese hypotensor that clinical efficacy is very definite simply.China develops the Chinese patent drug such as folk prescription tablet, medicinal tea, capsule, granule (electuary) and compound preparation " kendir pressure-reducing sheet ", " FUFANG LUOBUMA PIAN ", " compound Herba Apocyni veneti (Folium Apocyni Veneti) particle (electuary) " in succession, and function cures mainly and is hypertension and caused dizziness thereof and insomnia.

Dong Zhongtian (Dong Zhongtian, Hou Xinrong, Zhao Shufen. drug-metabolizing enzymes is to the hemodynamic effect of anesthetized dog. Capital Medical College's journal, 1987,8 (2): 109-112) etc. have studied the hemodynamic effect of drug-metabolizing enzymes to anesthetized dog: Folium Apocyni Veneti medicinal extract provides (the suitable crude drug in whole 1000g of 125g) by Dongtai bluish dogbane factory, make 10% distilled water immersion liquid, after filtration, by medicinal extract 200mg/kg, 3.6mL speed per minute is injected to anesthetized dog vein constant speed, result blood pressure declines and continues more than 7min, and declining maximumly reaches 22.3%.

Fragrant river pearl reality (He Yuzhuo. the hypotensive effect (1) of dogbane leaf extractive. foreign medical science traditional Chinese medicine fascicle, 2005,27 (3): 176-177) research to dogbane leaf extractive hypotensive effect such as: freely absorb the powder feed 7 weeks containing 1.25%, 2.5%, 5% dogbane leaf extractive (containing flavonoid components Hyperoside and each 6mg/g of isoquercitrin) to original hypertensive rat, when the 5th week, 2.5%, 5% group of blood pressure significantly reduces; Within 6th week, respectively organize blood pressure all significantly to reduce; After 7 weeks, drug withdrawal 2 weeks, can have lasting hypotensive effect during this period, have no the rebound phenomena after drug withdrawal; Each group of body weight and equal indifference between food ration and control group; Normal rat freely absorbs the powder feed 6 weeks containing 5% dogbane leaf extractive, has no the impact on blood pressure, has no difference between chow diet group body weight and dogbane leaf extractive group; Result shows that dogbane leaf extractive only acts on the blood pressure that autonomic nervous dysfunction causes and raises, on stable blood pressure without impact, on the body weight, food ration etc. of animal all without affecting.

Fragrant river pearl reality (He Yuzhuo. the hypotensive effect (2) of dogbane leaf extractive. foreign medical science traditional Chinese medicine fascicle, 2005,27 (3): 177-178) research to dogbane leaf extractive hypotensive effect such as: to (id) in spontaneous hypertensive rat duodenum give successively dogbane leaf extractive 30,100,300,1000mg/kg, about 10min starts to occur hypotensive effect, the time point of maximum reducing effect is 30 ~ 40min after administration, occurs mild drawdown curve; The maximum reducing value of 30 ~ 300mg/kg increases to 2.80kPa from 1.20kPa, but heavy dose of (1000mg/kg) has no hypotensive effect enhancing; Each group all has no significant effect norepinephrine (Nad) boosting and acetylcholine (Ach) step-down; Dogbane leaf extractive (300,1000mg/kg) afterwards 10,30 and 50min is given to SD system rat id, iv gives angiotensinⅠ 0.3 μ g/kg, result gives the boosting of dogbane leaf extractive to angiotensinⅠ in advance, each time point is showed no obvious inhibitory action, and gives each dosage dogbane leaf extractive and iv in advance and give physiological saline to the blood pressure of SD system rat also all without impact; Show dogbane leaf extractive and act on medicine and the food of sympathetic nervous system or parasympathetic system or drink water without interacting, its hypotensive effect is not based on the inhibitory action to sympathetic nervous system and ACE.

He Nianshan (He Nianshan, Zhang Yanmei. the routine clinical observation of bluish dogbane compound tea treatment hyperpiesia 56. Sichuan traditional Chinese medical science .2004, 22 (7): 49-50) etc. hypertensive clinical research is treated to bluish dogbane compound tea: with the bluish dogbane compound tea developed voluntarily (by bluish dogbane 50g, ginkgo leaf 15g, hawthorn 20g, chrysanthemum 10g, green tea 5g forms, every bag containing crude drug 5g, each 1 bag, 2 times on the 1st) treatment hyperpiesia 56 example (treatment group), and compare with Niuhuang Jiangya pill for curing 52 example (control group), continuous use 8 weeks, application blood pressure device timing measuring blood pressure observes its curative effect.Result shows, treatment group efficacy of antihypertensive treatment is 87.5%, is better than 73.1% of control group, and between group, comparing difference has conspicuousness (P<0.05).

Yu Yingying (Yu Yingying, Wang Haiming, Tang Xiuqiong, Deng. Folium Apocyni Veneti is as the safety evaluatio of drink tea. toxicology magazine .2006,20 (2): 134) etc. to the research of Folium Apocyni Veneti as the security of drink tea: bluish dogbane stem tea (Lv Kang bluish dogbane Co., Ltd provides by Xinjiang) is soaked 2 times with boiling water, merge soak in 60-70 DEG C of water bath with thermostatic control, be concentrated into the leachate be equivalent to containing stem tea 0.25,0.5 and 1.0g/mL respectively, stand-by.It is 1.00,2.15,4.64 and 10.0g/kg that reference sample human body maximum recommended dosage (6.0g/60kg) sets animal acute oral toxicity test given low; Other every animal experiments all by 25,50 and 100 times of human body RD namely 2.50,5.0 and 10.0g/kg establish 3 dosage groups.Negative control group gives drinking-water.Safety evaluatio test is carried out according to " toxicological evaluation of food safety procedure and method (GB151931-1994) ".Result: dogbane tea is to Kunming mouse and Wistar rat acute per os LD ₅₀all be greater than 10.0g/kg, within 2 week experimental period, all do not occur that abnormal symptom is with dead; The test of Micronuclei In The Mouse Bone Marrow rate, mouse inbred strain, Teratogenicity test are compared with negative control group, and there are no significant for difference; Rat 30d feeding trial each dosage group growing state and food utilization are compared with control group, and there are no significant for difference; Hematological indices, biochemical index, dirty body when histopathologic examination are showed no exception.Show that bluish dogbane is the natural plant tea of safe for drinking, can be used as natural cardiovascular health-care function vigor composition in addition extensive exploitation utilize.

The bark of eucommia is Chinese Famous " traditional Chinese medicines ", Shennong's Herbal is classified as top grade, Compendium of Material Medica is recorded " bark of eucommia is pungent flat, nontoxic; cure mainly waist and knee, tonifying middle-Jiao and Qi, hard muscles and bones, Qiang Zhi, wet except itching under the moon; antiabortive; clothes are made light of one's life by commiting suicide resistance to old for a long time ", and says its leaf name " cotton bud ", decocts soup or makes cooked food.At the beginning of the fifties in last century, Soviet Union scholar has found that the bark of eucommia is the active drug of Hypertension.Afterwards because Bark of Eucommia Ulmoides medicine source is under-supply, normal with Ye Daipi use clinically, effect is still good.Nowadays, folium cortex eucommiae loaded " Chinese Pharmacopoeia " (version in 2005), has " bark of eucommia electuary ", " bark of eucommia plain presser ", " bark of eucommia is two falls bagged instant preparation " etc. containing the step-down Chinese patent drug of folium cortex eucommiae.

Chen Lian (Chen Lian, Chang Furong, Wang Dianjun, Deng. contrast experiment's research of Bark of Eucommia Ulmoides and leaf. Nanjing Traditional Chinese Medical College journal .1986,: 40-43) etc. (2) to contrast experiment's research of Bark of Eucommia Ulmoides and leaf: Bark of Eucommia Ulmoides and Ye Jun to be made water decoction and 65% ethanol extract, the concentration of two kinds of samples is all 1 milliliter (suitable crude drug 3 grams), inject to Anaesthetic Rabbits ear vein by 2mL/kg, result skin and leaf all have obvious hypotensive effect to the blood pressure of rabbit, and both are without significant difference; Press 1mL/kg to the intravenous injection of anesthesia dog with the alcohol extract of Pi Heye, result also all has obvious hypotensive effect, and both are without significant difference.

Huang wu light (Huang wu light, Zeng Qingzhuo, Pan Zhengxing, Deng. folium cortex eucommiae electuary Pharmacodynamics and studies on acute toxicity. Guizhou medicine .2000,24 (6): 325-326) etc. on folium cortex eucommiae electuary Pharmacodynamics and studies on acute toxicity: measure folium cortex eucommiae medicinal extract (being provided by Guizhou Minghu Pharmaceutical Co., Ltd.) to the impact of anesthetized cat blood pressure with RM-6000 type channel polygraph, three dosage groups (0.25,0.5,1.0g/kg) all have obvious hypotensive effect as a result, and intensity dosage increases and increases, step-down is held time and is also extended thereupon.Carry out acute toxicity testing with folium cortex eucommiae electuary Cmax (1g/mL), ig is administered once (0.8mL/ only) on an empty stomach, and in 7 days as a result, mouse physiological status is all normal, and none is dead.It is 40g/kg that the once oral maximal tolerance dose of electuary is tried to achieve in experiment, shows that this product toxicity is low, takes safety.

Dang Zhongqin (Dang Zhongqin, Dang Zhongfang. the routine clinical observation of bark of eucommia blood vessels peace capsule for treating high blood pressure 82. Chinese traditional Chinese medicine information magazine .1998, 5 (7): 35-36) etc. to the routine clinical observation of bark of eucommia blood vessels peace capsule for treating high blood pressure 82: application is from intending bark of eucommia blood vessels peace capsule (by the bark of eucommia, the root of herbaceous peony, radix achyranthis bidentatae, the root of kudzu vine, Huai Chrysanthemi, raw hawthorn, rhizoma Gastrodiae, the medicine compositions such as rhizoma alismatis, Preparation Room in Hospital is processed), 5 tablets/time, 3 times/day, Hypertension 82 example, Stochastic choice 30 example is control group simultaneously, give FUFANG LUOBUMA PIAN, 2 pieces/times, 3 times/day.It within 4 weeks, was 1 course for the treatment of.Result: treatment group step-down total effective rate is 92.68%, control group is 76.67%; Treatment group lipopenicillinase total effective rate is 95.24%, and control group is 66.67%; Treatment group symptom total effective rate is 97.56%, and control group is 83.33%.Show that bark of eucommia blood vessels peace capsule is improved symptom obvious, hypotensive effect is lasting, and the advantages such as lipopenicillinase is remarkable, all cases does not all find bad reaction.

Rhizoma Gastrodiae is calming the liver to stop the wind by ancient Chinese medicine doctor is praised highly, and be the key medicine that treatment headache is dizzy, Shennong's Herbal is classified as top grade, Compendium of Material Medica is recorded " pungent, temperature, nontoxic.The beneficial strength of clothes of a specified duration, long cloudy, fertile strong, make light of one's life by commiting suicide, increase year.Disappear too fat to move, lower limb are full, blood under cold hernia.Cure mainly rheumatism, spasm of limbs, paralyse unsuccessful; Children's's wind pain, frightened gas, aid yang qi, mends five earth sevens kinds of impairments; The empty dizziness and headache of wind, promoting blood circulation, has one's ideas straightened out.Take Wuji ", and call it " medicine that Liver Channel gas divides "." all wind trembling and dizziness all belong to wood to " Plain Questions " cloud.Therefore rhizoma Gastrodiae enters to faint the moon through and control all diseases." bibliographical information, the depressor containing rhizoma Gastrodiae has " Tianma Gouteng Yin ", " compound Tianma granules ", " powerful gastrodia elata-cortex capsule " etc.

Chen Yun's cloud (Chen Yun's cloud, Liu Zhongqing, Chang Qi, Deng. the clinical observation of gastrodia tuber antihypertensive capsule for treating essential hypertension in 81 patients. Anhui tcm clinical practice magazine .2002,14 (5): 336-337) etc. to the clinical observation of gastrodia tuber antihypertensive capsule for treating essential hypertension in 81 patients: by 182 routine primary hypertension patients, be divided into 2 groups at random, treatment group 81 example, gastrodia tuber antihypertensive capsule is adopted (to be made up of Chinese medicines such as rhizoma Gastrodiae, the root of bidentate achyranthes, folium cortex eucommiae, the red sage root, Radix Aristolochiaes, every capsules 0.35g, is equivalent to crude drug amount 5g, oral, 3 times on the 1st, each 3) treatment; Control group 81 example, adopts FUFANG LUOBUMA PIAN (3 times on the 1st, each 2) treatment; Equal 4 weeks was 1 course for the treatment of, Timing measurement blood pressure before and after treatment.Result treatment group obvious effective rate is all significantly higher than control group (P<0.05), is also better than control group, does not find obvious adverse reaction in therapeutic process to the improvement result of symptoms such as having a headache, dizzy.Illustrate that gastrodia tuber antihypertensive capsule for treating essential hypertension is safe and effective.

Chen Xinyu (Chen Xinyu, Guo Zhenqiu, Zhu Meiju, Deng. stress high blood pressure and the impact on Angiotensin II thereof are treated in the flat liver ruling by law of nourishing the liver clearing liver. Hunan College of Traditional Chinese Medicine journal .2001, 21 (4): 41-44) etc. stress high blood pressure and the impact on Angiotensin II are treated on the ruling by law of nourishing the liver clearing liver flat liver: hyperactivity of yang due to yin deficiency or overabundant liver-fire are belonged to 60 routine differential diagnosis in tcm, the primary hypertension patient of stress test (MOST) positive carries out clinical observation on the therapeutic effect, result shows: use the mediodespidine capsule with the flat liver effect of nourishing the liver clearing liver (by rhizoma Gastrodiae, spina date seed, the compositions such as chrysanthemum, every 0.43g, by No.1 Hospital Attached to Hunan College of Traditional Chinese Medicine system, lot number 990503, 5 pieces/times, 3 times/day) treatment, its efficacy of antihypertensive treatment, symptom curative effect and total clinical efficacy aspect and control group Verapamil have suitable effect, and the blood pressure level that can effectively reduce after cold pressor test, reducing heart rate, reduces Angiotensin II level, thus reaches the degree reducing sympathetic activation, lowers the increasing degree of unwanted blood pressure.

Wang Xiaye (Wang Xiaye. powerful gastrodia elata-cortex capsule Hypertension liver-yang hyperactivity disease observation of curative effect. Heilungkiang traditional Chinese medicine .2004, (4): 5) to powerful gastrodia elata-cortex capsule Hypertension liver-yang hyperactivity disease observation of curative effect: choose high blood pressure liver-yang hyperactivity disease patient 66 example, be divided into treatment group 5 example at random, control group 31 example.Control group gives Lotensin sheet, 10mg, oral once a day, while treatment group gives Lotensin sheet, gives powerful gastrodia elata-cortex capsule (being made up of rhizoma Gastrodiae, the bark of eucommia etc., 2 tablets/time, 3 times/day) every day oral, 3 weeks courses for the treatment of.Observe blood pressure, the symptom such as dizzy improves situation.Result: treatment group and control group are all improved effect to These parameters, but treatment group improvement is obviously better than control group.

Sophora flower is considered as " cool blood key medicine " by ancient Chinese medicine doctor, and the light gas of its body is thin, and property master is descending, and under rushing down on kind clear, cool blood consolidating the yin, can let out lung inverse, fall irascibility, the cool large intestine of purge the sthenic heart-fire, hard kidney water, yin-nourishing blood, is the cloudy blood system medicine of yangming syncope.Modern pharmacological research shows, rutin sophorin contained by sophora flower, can strengthen capillary resistance, improves vascular wall fragility, hyperpietic is had to the effect preventing rupture of blood vessel in brain.

Zhao Yunzhi (Zhao Yunzhi. from intend sophora flower drink prevent and treat hypertension, hyperlipidemia. Journal of Traditional Chinese Medicine .2007,48 (11): 1006) from intend sophora flower drink prevent and treat vascular hypertension: Lee so-and-so, man, 52 years old, first visit on March 6 in 2002.Patient is not recently relaxed because of feelings will and is caused dizziness and distending sensation of the head, hot eyes, being irritable and getting angry easily, sleep dreaminess, dry mouth with bitter taste, dry and hard excrement, scanty drak urine, goes to a doctor, give Longdan Xiegan wan oral to unit clinic.Hot eyes, dry hardship after medicine, constipation urine is red waits disease alleviation, still feels dizziness and distending sensation of the head, two xerophtalmias, blurred vision, examines and see that patient's body is more fat, flushed face, and it is dizzy heavy and swollen to state, blood pressure 150/100mmHg, red tongue body, thin lingual fur Huang, wiry and rolling pulse.Requirement takes traditional Chinese medicine treatment, and give sophora flower drink (sophora flower 15g, raw hawthorn 10g, chrysanthemum 9g), water logging is for tea-drinking, and every day 1 dose, take 20 days, it is sharp clearly that patient feels the head, blood pressure 140/95mmHg; Following the service 20 doses, blood pressure recovers normal, feels without significant discomfort.

Zheng Weisheng (Zheng Weisheng. sophora flower is cured the disease proved recipe. and Reading is always profitable: seek medical advice and medicine .2008, (7): 47) report " sophora flower cure the disease proved recipe ": high blood pressure, sophora flower 12g, green tea 20g, mixing, put in thermos bottle, brew with boiling water, after covering vexed more than 10 minutes, again and again drink, renew after having drunk and wash water open, every day 1 dose.

Xu Jingyu (Xu Jingyu, Su Runze, Zhang Liang. compound sophora flower anti-hypertensive electuary treatment insulin resistance hypertension. combination of Chinese tradiational and Western medicine cardiovascular and cerebrovascular diseases magazine .2005,3 (6): 489-490) etc. with compound sophora flower anti-hypertensive electuary treatment insulin resistance hypertension: 52 routine inpatients to be divided at random compound sophora flower anti-hypertensive electuary group (30 example) and control group (22 is routine), select physical examination of healthy population (healthy group) 17 same period, before observing 3 groups of treatments respectively and 4 weeks blood pressures, blood fat, glucose tolerance change after treatment.Treatment group gives compound sophora flower anti-hypertensive electuary (sophora flower, chrysanthemum, selfheal, Cassia occidentalis, parasitic loranthus equivalent, Pharmacy of the attached First Academy of Heilongjiang University of Chinese Medicine provides, 1mL is containing said medicine 5mg,), control group gives enalapril maleate (Yangtze River medicine company), each 12.5mg-25.0mg, every day 2 times.Two groups of courses for the treatment of are 4 weeks.Result: patient's blood fat for the treatment of group and control group, glucose tolerance, apparently higher than healthy group, illustrate that hypertension patients compares with Healthy People and there is lipid, dysglycemia.Not statistically significant is compared in the effect in step-down for the treatment of group and control group, and lipopenicillinase, hypoglycemic aspect are better than control group.Show compound sophora flower anti-hypertensive electuary treatment insulin resistance hypertension determined curative effect, and there is the effect of adjusting blood lipid, hypoglycemic.

Chrysanthemum is as medicinal, and head sees Shennong's Herbal, Compendium of Material Medica is recorded " chrysanthemum cure mainly all dizzy, support order blood, take sharp vim and vigour for a long time, make light of one's life by commiting suicide and resistance toly always to prolong life ".It is dizzy that the headache caused for liver-yang hyperactivity clinically and kidney deficiency and liver cause, and quite has effect.

Wear and quickly (wear quick, Liu Qingyun, Li Daozhong, Deng. chrysanthemum is antipyretic, the research of the material base of hypotensive effect. Chinese medicine .2001,24 (7): 505-506) the material base research of, hypotensive effect antipyretic to chrysanthemum such as: get rat 42, be divided into 7 groups at random, blank (physiological saline group), solvent (Tween-80) group, chrysanthemum total flavone group, chrysanthemum total flavone monomer acacetin-7-0-glucoside (F1) group, chrysanthemum total flavone monomer apiolin-7-0-glucoside (F2) group, chrysanthemum ligroin extraction group, volatile oil of chrysanthemum group.Pressure value before every rat recording medicine, distinguish intravenous injection physiological saline, Tween-80, general flavone solution, F1 solution, F2 solution, ether extract solution, the equal 0.8mg/kg of volatile oil again, pressure value when 20min, 40min, 60min, 80min, 120min after recording medicine.As a result, physiological saline group pressure value is basicly stable before and after medication, shows that blood pressure measuring method is reliable; Before and after Tween-80 group, pressure value is without obvious change, illustrates that this solution is little on blood pressure impact; After intravenous injection general flavone, during 40min to 80min, blood pressure significantly declines, and F2 group blood pressure when 60min has reduction trend, and other respectively to organize before and after medication pressure value without obvious change.Research shows, chrysanthemum total flavone extract has significant antihypertensive effect to rat, general flavone monomer apiolin-7-0-glucoside (F2) has certain hypotensive effect trend, the active ingredient of prompting hypotensive effect is total flavonoids substance, may be the synergistic result of each monomer component.

(Fan family selects Fan family's choosing; Zangwen China. Huai Chrysanthemi medicinal extract is on the impact of spontaneous hypertensive rat blood pressure and target organ lipid peroxidation thereof. Henan College Of Traditional Chinese Medicine journal .2006; 21 (126): 18-19) etc. have studied the impact of Huai Chrysanthemi medicinal extract on spontaneous hypertensive rat blood pressure and target organ lipid peroxidation thereof: Huai Chrysanthemi medicinal extract concentration is 3.3g crude drug/mL; 40 spontaneous hypertensive rats are divided at random hypertension control group, Bezoar pill for lowering blood pressure group, Huai Chrysanthemi medicinal extract high dose group, Huai Chrysanthemi medicinal extract low dose group, often organize 10.Separately set 10 Wistar rats as Normal group.Each experimental group all adopts the administration of gavage mode, and administration time is 70d, and every day 1 time, all in morning dose every day.Normal group and hypertension control group gavage distilled water (10mL/kg), and Huai Chrysanthemi high dose group dosage is 33.0g/kg/d, and Huai Chrysanthemi low dose group dosage is 16.5g/kg/d, and Bezoar pill for lowering blood pressure group given low is 1g/kg/d.After testing 70d Measure blood pressure, each group of rat is put to death, get portion of tissue block (heart, brain, kidney), measure the content of MDA (MDA) and hepatocuprein (SOD).Result from be administered to administration and terminate, hypertension control rats blood pressure level all has significant difference (P<0.01) compared with Normal group.During administration 0d, each administration group is similar with blank group blood pressure level, compares there was no significant difference (P>0.05).When being administered to 70d, Bezoar pill for lowering blood pressure group, Huai Chrysanthemi high dose group blood pressure level have significant difference (P<0.01) compared with hypertension control group.Compare with Normal group, the hypertension control group heart, brain, nephridial tissue MDA content significantly raise, and SOD content obviously reduces, difference highly significant (P<0.01).Compare with hypertension control group, Bezoar pill for lowering blood pressure group, Huai Chrysanthemi high dose group all significantly can increase the Hypertensive Rats heart, brain, nephridial tissue SOD level, obviously reduce MDA content (P<0.01).Show, Huai Chrysanthemi medicinal extract high dose group can reduce spontaneous hypertensive rat blood pressure level, has obvious inhibitory action to target organ lipid peroxidation injury simultaneously, also may inhibit the pathologic process that free radical causes elevation of the blood pressure.

(local records peak, local records peak, Huang Lin, Li Qinghua, Deng. notoginseng chrysanthemum tea electuary assisting in treating hypertension effect observation. Guangxi preventive medicine .2004, 10 (3): 174-175) etc. to notoginseng chrysanthemum tea electuary assisting in treating hypertension effect observation: to the primary hypertension patient of 65 example ages at 18 to 65 years old, take notoginseng chrysanthemum tea continuously (containing sanchi flower, chrysanthemum, tea extract, every bag of 3g, warm water electuary, each 1 bag, every day 2 times) 30 days, observe experimenter and take anteroposterior contraction pressure, diastolic pressure, clinical symptoms, the index changes such as routine blood test and biochemistry.After result experimenter test-meal, clinical symptoms integration obviously reduces (P<0.01); Systolic pressure and diastolic pressure all obviously decline (P<0.01), and routine blood test and biochemical indicator are all without change.Show, notoginseng chrysanthemum tea electuary has the effect of aided blood pressure-lowering.

China is the country of gathering and processing and drinking tealeaves in the world the earliest, has the tea culture of thousands of years, and Shennong's Herbal is recorded, and " shennong went into the mountains collecting, tasting and testing different kinds of herbs to be used as medicine, meets 72 poison day, obtain tea and separate it.", " tea history " carry " tea for drink, send out Shen Nongshi, hear in Lu Zhougong.There is Yan Ying together; The Chinese has Yang Xiong, Si Maxiangru; Wu has sunlight Wei; Shanxi have Liu Kun, carry, Lu Na, Xie An, left think of apprentice here all drink.", entering Tang has Lu Yu " tea warp " to come out.Now, tea has become one of large beverage in the generally acknowledged world three, and a lot of the elderly will drink tea and to be accustomed to as one way of life.Green tea is also known as azymic tea, and compared with general ripe tea, as black tea and oolong tea, green tea is without oxidation, and remain more natural anti-oxidation composition, wherein Tea Polyphenols retains more than 85% of fresh leaf.

Jin Xingyong (Jin Xingyong. the health care of Tea Polyphenols and the application on food thereof. Food Additives Used in China .1995, (2): 27-31) report the health care of Tea Polyphenols and the application on food thereof: green tea polyphenols have antibacterial, preventing decayed tooth, norcholesterol, hypotensive, delay senility, the effect such as anti-oxidant; Natural Antioxidant Tea Polyphenol be used for hypertension, coronary heart disease prevention or to share with the medicine of hypertension, coronary heart disease be up-and-coming.

Ke Yongsheng (Ke Yongsheng, Cao Heng, Xu Xiaohua, Deng. Tea Polyphenols is on primary hypertension patient oxygen radical and hemorheological impact. Chinese combination of Chinese tradiational and Western medicine magazine .1997, 17 (4): 221-223) etc. have studied Tea Polyphenols to primary hypertension patient oxygen radical and hemorheological impact: (every containing Tea Polyphenols 25mg to observe tea-polyphenol capsules, each 2, every day 3 times, totally 4 weeks) on primary hypertension patient (38 example) oxygen radical and hemorheological impact, and with enteric coated aspirin (each 75mg, every day 1 time, totally 4 weeks) 130 examples for the treatment of compare.Result Tea Polyphenols can significantly improve red blood cell activity of SOD, reduces serum malondialdehyde, reduces whole blood contrast viscosity, plasma viscosity and plasma fibrinogen level.Its pharmacodynamics effect is better than enteric coated aspirin.

Wang Jian (Wang Jian. hypertensive green tea therapy. tea machinery magazine .1999, (3): 38) report hypertensive green tea therapy: senior green tea 6g, folium cortex eucommiae 6g, boiling water brews and drink-service after adding a cover 5min, every day 1 dose, to hypertension and heart disease complication effective in cure.

The tribute Chinese female (the tribute Chinese is female. sophora flower chrysanthemum green tea beverage. food and machinery .2002, (5): 26,40) report sophora flower chrysanthemum green tea beverage: with sophora flower, chrysanthemum and green tea for primary raw material, make a kind of applicable old man to drink, to relieve summer heat the generation and development of quenching one's thirst, delay and alleviating geriatric disease, to ensure the able-bodied health drink of the elderly.In optimum formula, every 1kg finished product sophora flower 4g, chrysanthemum 4g, green tea 4g.

Folium Apocyni Veneti meridian distribution of property and flavor is sweet, bitter, cold, returns Liver Channel.There is flat liver calm the nerves, the effect of clearing away heat and promoting diuresis, for dizziness due to hyperactivity of liver-YANG, palpitation and insomnia, edema oliguria; Hypertension, neurasthenia, ephritis edema.Pharmacological research proves, Folium Apocyni Veneti has the effects such as step-down, tranquilizing and allaying excitement, lipopenicillinase, anti-oxidant, diuresis, Liver protection, enhancing are immune, antiviral, anti-diabetic vascular lesion.Specify in China's pharmacopeia, consumption is 6 ~ 12g.

Folium cortex eucommiae meridian distribution of property and flavor is micro-pungent, and temperature, return liver, kidney channel, have filling liver kidney, the effect of strengthening the bones and muscles, for kidney deficiency and liver, have a dizzy spell, soreness of waist and knee joint, muscles and bones impotence is soft.Pharmacological research proves, folium cortex eucommiae all has significant pharmacological action to the cardiovascular system of body, immune system, have step-down, immunity moderation, antitumor, promote the effects such as bone cell proliferation.Specify in China's pharmacopeia, consumption is 10 ~ 15g.

Rhizoma Gastrodiae meridian distribution of property and flavor is sweet, flat, returns Liver Channel, has the effect that suppressing hyperactive liver for calming endogenous wind stops convulsion, dizzy for having a headache, extremity numbness, child convulsion, epilepsy clonus, lockjaw.Pharmacological research proves, rhizoma Gastrodiae has the multiple effects such as step-down, scavenging free radicals, enhancing immunity.Specify in China's pharmacopeia, consumption is 3 ~ 9g.

Sophora flower meridian distribution of property and flavor is bitter, is slightly cold, returns liver, large intestine channel, have cooling blood and hemostasis, the effect of clearing liver-fire, and for having blood in stool, hemorrhoid blood, bloody flux, uterine bleeding, spit blood, bleeding from five sense organs or subcutaneous tissue, the hot hot eyes of liver, has a headache dizzy.Pharmacological research proves, sophora flower has the effect such as anti-inflammatory, spasmolysis, step-down, lipopenicillinase.Specify in China's pharmacopeia, consumption is 5 ~ 9g.

Chrysanthemum meridian distribution of property and flavor is sweet, bitter, is slightly cold, and returns lung, Liver Channel, has loose wind heat-clearing, the effect of flat liver improving eyesight, and for anemopyretic cold, have a headache dizzy, red eye, swell pain, eyes is dim-sighted.Pharmacological research proves, chrysanthemum has throat-clearing throat-moistening, antipyretic, step-down, the effect such as anti-ageing, antitumor.Specify in China's pharmacopeia, consumption is 5 ~ 9g.

Green tea is the one of tealeaves, and tealeaves has the use history of several thousand in China, says in the Tang Dynasty Chen Cangqi supplement to the Herbal: " all medicines are the medicine of each medicine, and tea is the medicine of ten thousand medicines." Ming Dynasty turns round and look at unit's celebrating tea spectrum and records: " drink genuine tea can be quenched the thirst, helped digestion, eliminating phlegm, sleep less, diuresis road, improving eyesight benefit think of, relieving restlessness, go greasy, people admittedly can not one day without tea." pharmacological research proves, green tea has antibacterial, antiviral, anti-oxidant, step-down, lipopenicillinase, improves gut flora, the anti-ageing effect of waiting for a long time.Green tea is bread and cheese, and edible safety limits without consumption.

Although there is the continuity traditional Chinese medicine theory of thousands of years in China, although and each material of the present composition has reported hypotensive activity more or less, but each material combinations of the present composition there is no any theory of traditional Chinese medical science of method explains together, these are just bound to present additive effect hypotensive activity when report has the material combinations of hypotensive activity together more or less separately cannot be predicted.

Detailed description of the invention

The following examples provided only for task of explanation instead of for, should not be interpreted as limiting the present invention by any way yet.Those skilled in the art will recognize that can make routine to following examples when not surmounting the spirit or scope of the present invention changes and amendment.Below prepare in the example of composition, although represent recipe quantity with weight portion, if not otherwise indicated, with the preparation of the amount of the rhizoma Gastrodiae 2kg that feeds intake, in last packaging product, in every bag, contain rhizoma Gastrodiae 0.2g.The each composition below prepared can be described as two numb tea containing leaves of Eucommia ulmoides or can be described as the two numb tea containing leaves of Eucommia ulmoides of your health version old.

One, the embodiment of composition is prepared

Embodiment 1:

Prescription:

rhizoma Gastrodiae	200 weight portions,
		folium Apocyni Veneti	800 weight portions,
folium cortex eucommiae	500 weight portions,
		sophora flower	300 weight portions,
chrysanthemum	200 weight portions,
		green tea	1000 weight portions.

Method for making:

Embodiment 2:

Prescription:

rhizoma Gastrodiae	200 weight portions,
		folium Apocyni Veneti	880 weight portions,
folium cortex eucommiae	450 weight portions,
		sophora flower	330 weight portions,
chrysanthemum	180 weight portions,
		green tea	1100 weight portions.

Method for making:

Embodiment 3:

Prescription:

rhizoma Gastrodiae	200 weight portions,
		folium Apocyni Veneti	720 weight portions,
folium cortex eucommiae	550 weight portions,
		sophora flower	270 weight portions,
chrysanthemum	220 weight portions,
		green tea	900 weight portions.

Method for making:

Embodiment 4:

Prescription:

rhizoma Gastrodiae	200 weight portions,
		folium Apocyni Veneti	600 weight portions,
folium cortex eucommiae	625 weight portions,
		sophora flower	225 weight portions,
chrysanthemum	250 weight portions,
		green tea	750 weight portions.

Method for making:

Embodiment 5:

Prescription:

rhizoma Gastrodiae	200 weight portions,
		folium Apocyni Veneti	1000 weight portions,
folium cortex eucommiae	375 weight portions,
		sophora flower	375 weight portions,
chrysanthemum	150 weight portions,
		green tea	1250 weight portions.

Method for making:

Embodiment 6:

Prescription:

rhizoma Gastrodiae	200 weight portions,
		folium Apocyni Veneti	1200 weight portions,
folium cortex eucommiae	250 weight portions,
		sophora flower	450 weight portions,
chrysanthemum	100 weight portions,
		green tea	1500 weight portions.

Method for making:

Embodiment 7:

Prescription:

Rhizoma Gastrodiae

200 weight portions,

folium Apocyni Veneti	400 weight portions,
		folium cortex eucommiae	750 weight portions,
sophora flower	150 weight portions,
		chrysanthemum	300 weight portions,
green tea	500 weight portions.

Method for making:

Embodiment 11:

Prescription:

Method for making:

(3) by through the Folium Apocyni Veneti of pre-treatment, folium cortex eucommiae, rhizoma Gastrodiae, sophora flower, green tea and chrysanthemum, pulverize respectively, with 24 orders and the screening of 65 eye mesh screens, get each meal, mix with step (3) gained meal, 60 DEG C of dry 3h, total mixed 30min, carry out composite film packaging with packaging machine of tea bag, 6kGy irradiation sterilization, to obtain final product.

Embodiment 12:

Prescription:

Method for making:

(3) pulverize through the Folium Apocyni Veneti of pre-treatment, folium cortex eucommiae, rhizoma Gastrodiae, sophora flower, green tea together with chrysanthemum, with 24 orders and the screening of 65 eye mesh screens, get each meal, mix with step (3) gained meal, 60 DEG C of dry 3h, always mixed 30min, carries out composite film packaging with packaging machine of tea bag, 6kGy irradiation sterilization, to obtain final product.

Embodiment 13:

Prescription:

Method for making:

(3) take sophora flower by formula rate to pulverize together with chrysanthemum, with 24 orders and the screening of 65 eye mesh screens, get meal;

(4) by Folium Apocyni Veneti, folium cortex eucommiae, rhizoma Gastrodiae, the green tea through pre-treatment, pulverize respectively, with 24 orders and the screening of 65 eye mesh screens, get each meal, mix with step (3) gained meal, 60 DEG C of dry 3h, total mixed 30min, carry out composite film packaging with packaging machine of tea bag, 6kGy irradiation sterilization, to obtain final product.

Embodiment 14:

Prescription:

Method for making:

(3) take Folium Apocyni Veneti by formula rate to pulverize together with chrysanthemum, with 24 orders and the screening of 65 eye mesh screens, get meal;

(4) by green tea, folium cortex eucommiae, rhizoma Gastrodiae, the sophora flower through pre-treatment, pulverize respectively, with 24 orders and the screening of 65 eye mesh screens, get each meal, mix with step (3) gained meal, 60 DEG C of dry 3h, total mixed 30min, carry out composite film packaging with packaging machine of tea bag, 6kGy irradiation sterilization, to obtain final product.

Embodiment 15:

Prescription:

Method for making:

(3) take folium cortex eucommiae by formula rate to pulverize together with chrysanthemum, with 24 orders and the screening of 65 eye mesh screens, get meal;

(4) by Folium Apocyni Veneti, green tea, rhizoma Gastrodiae, the sophora flower through pre-treatment, pulverize respectively, with 24 orders and the screening of 65 eye mesh screens, get each meal, mix with step (3) gained meal, 60 DEG C of dry 3h, total mixed 30min, carry out composite film packaging with packaging machine of tea bag, 6kGy irradiation sterilization, to obtain final product.

Embodiment 16:

Prescription:

Method for making:

(3) take chrysanthemum by formula rate to pulverize, with 24 orders and the screening of 65 eye mesh screens, get meal;

(4) by Folium Apocyni Veneti, folium cortex eucommiae, rhizoma Gastrodiae, sophora flower, the green tea through pre-treatment, pulverize respectively, with 24 orders and the screening of 65 eye mesh screens, get each meal, mix with step (3) gained meal, 60 DEG C of dry 3h, total mixed 30min, carry out composite film packaging with packaging machine of tea bag, 6kGy irradiation sterilization, to obtain final product.

Embodiment 17:

Prescription:

rhizoma Gastrodiae	200 weight portions,
		folium Apocyni Veneti	400 weight portions,
folium cortex eucommiae	750 weight portions,
		sophora flower	150 weight portions,
chrysanthemum	300 weight portions,
		green tea	500 weight portions.

Method for making:

(3) take rhizoma Gastrodiae by formula rate to pulverize together with chrysanthemum, with 24 orders and the screening of 65 eye mesh screens, get meal;

(4) by Folium Apocyni Veneti, folium cortex eucommiae, green tea, the sophora flower through pre-treatment, pulverize respectively, with 24 orders and the screening of 65 eye mesh screens, get each meal, mix with step (3) gained meal, 60 DEG C of dry 3h, total mixed 30min, carry out composite film packaging with packaging machine of tea bag, 6kGy irradiation sterilization, to obtain final product.

Two, the assay of 3,5-O-DCQs in composition

Assay method:

Measure according to high performance liquid chromatography (annex VID);

Chromatographic condition and system suitability: take octadecylsilane chemically bonded silica as filler; Take acetonitrile as mobile phase A, with 0.1% phosphoric acid solution for Mobile phase B, the regulation according to the form below carries out gradient elution; Determined wavelength is 348nm; Number of theoretical plate calculates should be not less than 8000 by 3,5-O-DCQ peak;

The preparation of reference substance solution: get chlorogenic acid reference substance, galuteolin reference substance, 3, the two caffeoyl quinic acid reference substance of 5-O-is appropriate, accurately weighed, put in brown measuring bottle, add 70% methyl alcohol and make every 1ml containing chlorogenic acid 35 μ g, galuteolin 25 μ g, 3, the mixed solution of 5-O-DCQ 80 μ g, obtains (less than 10 DEG C preservations);

The preparation of need testing solution: get test sample in chrysanthemum about 0.25g, accurately weighed, put in tool plug conical flask, precision adds 70% methyl alcohol 25ml, close plug, weighed weight, ultrasonic process (power 300W, frequency 45kHz) 40 minutes, lets cool, weighed weight again, supply the weight of less loss with 70% methyl alcohol, shake up, filter, get subsequent filtrate, to obtain final product;

Determination method: accurate absorption reference substance solution and each 5 μ l of need testing solution respectively, injection liquid chromatography, measures, calculate the content of 3,5-O-DCQs in every 100g composition.

For the preparation of each composition of embodiment 1-7, embodiment 11-17, said method is used to monitor the content of 3,5-O-DCQs in various material.For each embodiment, get gained material after always mixing 30min in last step and measure the content of wherein 3,5-O-DCQs, count C0; Then measure the content carrying out 3,5-O-DCQs in gained bagged material when about 1/10 amount packed by total material in composite film packaging process in last step with packaging machine of tea bag, count C1, can be regarded as early stage content;

Then measure the content carrying out 3,5-O-DCQs in gained bagged material when about 5/10 amount packed by total material in composite film packaging process in last step with packaging machine of tea bag, count C2, can be regarded as content in mid-term;

Then measure and carry out total material in composite film packaging process with packaging machine of tea bag in last step and packed the content close to 3,5-O-DCQs in gained bagged material during performance, count C3, can be regarded as content in latter stage.

The early stage relative amount (X1) of 3,5-O-DCQs in the material of point process of assembling is calculated with following formula:

X1＝(C1÷C0)×100％

The relative amount in mid-term (X2) of 3,5-O-DCQs in the material of point process of assembling is calculated with following formula:

X2＝(C2÷C0)×100％

The relative amount in latter stage (X3) of 3,5-O-DCQs in the material of point process of assembling is calculated with following formula:

X3＝(C3÷C0)×100％

Above, X1, X2, X3 more show in the respective process of packing in material close to 100% 3,5-O-DCQ content consistent with time initial (it reflects the uniformity of chrysanthemum in material).

Result shows: for whole compositions of embodiment 11-17, their X1 is all in 98% ~ 102% scope, their X2 is all in 121% ~ 136% scope, their X3 is all in 89% ~ 93% scope, show in these embodiments, in point process of assembling, the uniformity of chrysanthemum in material changes along with point process of assembling, and this can cause the difference of packing work-in-process quality frequently; For whole compositions of embodiment 1-7, their X1, X2, X3, all in 98% ~ 102% scope, show in these embodiments, and in point process of assembling, the uniformity of chrysanthemum in material is good all the time.

Three, the animal experiment of two numb tea containing leaves of Eucommia ulmoides auxiliary function for lowering blood pressure

1 materials and methods

1.1 samples: embodiment 1 pair of numb Eucommia Tea.Human oral's recommended amounts is every day 3 times, each 1 bag, and adult's body weight press 60kg calculating, amounts to dosage 0.15g/kgbw.Sample processes by the following method: sample thief adds 10 times of water yields, normal pressure, 85 DEG C, soak 30 minutes, similarity condition extract twice, by extract merge, normal pressure, 85 DEG C be concentrated into every milliliter of concentrate and be equivalent to 1g sample.

1.2 animals used as test: the male spontaneous hypertension rat of SPF level (SHR) 40, is provided by Beijing Vital River Experimental Animals Technology Co., Ltd., animal used as test approval number SCXK (capital) 2007-0001.The male normal SD rats of SPF level 20, is provided by Henan Province's Experimental Animal Center, animal used as test production licence number SCXK (Henan) 2005-0001.The weight of animals is 180g ~ 220g.Feed is provided by Changsha Kaifu District Dong Chuan animal used as test Technical Services Division, production licence number SCXK (Hunan) 2006-0001.

1.3 experimental situation conditions: experimental situation is barrier system.Temperature 22 DEG C ~ 24 DEG C, humidity 52% ~ 58%, animal used as test occupancy permit number is SYXK (Hunan) 2005-0001.

1.4 dosage groupings and given the test agent give mode: arrange dosage with 5,10,30 times of the human body recommended amounts of embodiment 1 pair of numb Eucommia Tea, be respectively 0.75g/kgbw, 1.50g/kgbw, 4.50g/kgbw.During basic, normal, high dose of test liquid preparation, the numb Eucommia Tea of Example 1 pair soaks concentrate 7.5ml, 15.0ml, 45.0ml adding distil water to 100ml respectively, by 1.0ml/100gbw volume to spontaneous hypertension rat gavage, hypertension control group gavage gives isopyknic distilled water, once a day, continuous 30 days.Give normal SD rats by 1.0ml/100gbw volume gavage with 30 of human body recommended amounts times (4.50g/kgbw), Normal group gavage gives equal-volume distilled water, once a day, and continuous 30 days.

1.5 key instruments: electronic balance; 229-SSP type single channel without wound measuring blood pressure system, is manufactured by American I ITC LifeScience automatically.

1.6 experimental techniques:

The last week is carried out repeatedly blood pressure measurement to animal subject in experiment, makes it adapt to pressure measurement environment.Blood pressure is measured with tail pulses method.During mensuration, rat is fixed, under the environment of 29 DEG C ~ 31 DEG C, measure blood pressure and heart rate.Each measurement separated in time, recorded heart rate change≤10 beats/min, continuous three readings of blood pressure≤6mmHg, get its mean.Be divided into 3 dosage groups and 1 control group at random according to the basic blood pressure value of spontaneous hypertension rat, often organize 10; Be divided into 4.50g/kgbw dosage group and 1 control group at random according to the basic blood pressure of normal SD rats, often organize 10, dosage group gives tested material every day, and control group gives distilled water, continuous 30 days, weekly Measure blood pressure and heart rate and body weight 1 time.

1.7 experimental data statistics: carry out statistical analysis with Spss 11.0 software.First homogeneity test of variance is carried out to data, if variance is neat, adopts one-way analysis of variance totally to compare, find differences and carry out comparing between two between multiple dosage group with a control group mean by Dunnett method again.If heterogeneity of variance, then suitable variable transitions is carried out to initial data, after meeting homogeneity test of variance, add up by the data after conversion; If do not reach the neat object of variance after variable transitions yet, use rank test instead and add up, find overall more variant, then adopt and do not require that the Tamhane ' sT2 of homogeneity of variance inspection is compared between two.

1.8 results judge: test group of animals blood pressure is starkly lower than control group difference to be had conspicuousness and on the blood pressure of test group of animals heart rate and intact animal and heart rate without impact, can judge that auxiliary function for lowering blood pressure results of animal is positive.

2 results

The numb Eucommia Tea of 2.1 embodiment 1 pair is on the impact of spontaneous hypertension rat body weight

Table 1 embodiment 1 pair of numb Eucommia Tea is on the impact of spontaneous hypertension rat body weight

In whole process of the test, each treated animal well-grown is movable normal.From table 1, before and after test, each dosage group body weight and control group comparing difference are without conspicuousness (P > 0.05)

The numb Eucommia Tea of 2.2 embodiment 1 pair is on the impact of spontaneous hypertension rat blood pressure

Table 2 embodiment 1 pair of numb Eucommia Tea is on the impact of spontaneous hypertension rat blood pressure

Continued 2 embodiment 1 pair of numb Eucommia Tea is on the impact of spontaneous hypertension rat blood pressure

From table 2,4.50g/kgbw dosage group spontaneous hypertension rat tests last blood pressure lower than control group and difference has conspicuousness (P < 0.05), shows that embodiment 1 pair of numb Eucommia Tea can reduce the blood pressure of spontaneous hypertension rat.

The numb Eucommia Tea of 2.3 embodiment 1 pair is on the impact of spontaneous hypertension rat heart rate

Table 3 embodiment 1 pair of numb Eucommia Tea is on the impact of spontaneous hypertension rat heart rate

Continued 3 embodiment 1 pair of numb Eucommia Tea is on the impact of spontaneous hypertension rat heart rate

From table 3, experimental session, the heart rate of each dosage group spontaneous hypertension rat and control group comparing difference are without conspicuousness (P > 0.05).

The numb Eucommia Tea of 2.4 embodiment 1 pair is on the impact of normal SD rat body weight

Table 4 embodiment 1 pair of numb Eucommia Tea affects the body weight of normal SD rats

In whole process of the test, growth of animal is good, movable normal.From table 4, before and after test, 4.50g/kgbw dosage group body weight and control group comparing difference are without conspicuousness (P > 0.05).

The numb Eucommia Tea of 2.5 embodiment 1 pair is on the impact of normal SD rat blood pressure

Table 5 embodiment 1 pair of numb Eucommia Tea is on the impact of normal SD rat blood pressure

Continued 5 embodiment 1 pair of numb Eucommia Tea is on the impact of normal SD rat blood pressure

From table 5, there are no significant (P > 0.05) for the blood pressure of duration of test 4.50g/kgbw dosage group and control group comparing difference, shows embodiment 1 pair of numb Eucommia Tea to the blood pressure of normal SD rats without impact.

The numb Eucommia Tea of 2.6 embodiment 1 pair is on the impact of normal SD rat heart rate

Table 6 embodiment 1 pair of numb Eucommia Tea is on the impact of normal SD rat heart rate

Continued 6 embodiment 1 pair of numb Eucommia Tea is on the impact of normal SD rat heart rate

From table 6, there are no significant (P > 0.05) for the heart rate of duration of test 4.50g/kgbw dosage group and control group comparing difference, shows embodiment 1 pair of numb Eucommia Tea to the heart rate of normal SD rats without impact.

3 conclusions

Under this laboratory condition, with 0.75g/kgbw, 1.50g/kgbw, embodiment 1 pair of numb Eucommia Tea of 4.50g/kgbw dosage is to spontaneous hypertension rat (SHR) gavage 30 days continuously, with embodiment 1 pair of numb Eucommia Tea of 4.50g/kgbw dosage to the continuous gavage of normal SD rats 30 days, experiment end, the blood pressure of 4.50g/kgbw dosage group spontaneous hypertension rat is starkly lower than control group, difference has conspicuousness (P < 0.05), sample is to the heart rate of spontaneous hypertension rat, the blood pressure of body weight and normal SD rats, heart rate and body weight have no significant effect.According to the criterion of auxiliary function for lowering blood pressure in " health food inspection and assessment technical specification " (version in 2003), prompting embodiment 1 pair of numb Eucommia Tea has auxiliary function for lowering blood pressure to animal.

In addition, with reference to the method in the animal experiment of two numb tea containing leaves of Eucommia ulmoides auxiliary function for lowering blood pressure " three, " above, test port prosperous board gastrodia elata-cortex capsule, CN101455792A (200810159274.X) embodiment 1 gained powder, CN103610949A (201310693209.6) embodiment 1 gained capsule three (dosage is identical with the dosage in the animal experiment of two numb tea containing leaves of Eucommia ulmoides auxiliary function for lowering blood pressure " three, " above in rhizoma Gastrodiae); Result shows, and three kinds of compositions send out the blood pressure of Hypertensive Rats and control group there are no significant difference to be equivalent to 4.5g/kg.bw dosage group to blood at experiment end, show them and do not have auxiliary function for lowering blood pressure to animal.With reference to the method in the animal experiment of two numb tea containing leaves of Eucommia ulmoides auxiliary function for lowering blood pressure " three, " above, test each composition of embodiment 2-7 (dosage is identical with the dosage in the animal experiment of two numb tea containing leaves of Eucommia ulmoides auxiliary function for lowering blood pressure " three, " above in rhizoma Gastrodiae).Result shows, each composition all obtains the result substantially identical with embodiment, particularly, whole composition is at experiment end, the blood pressure of 4.5g/kg.bw dosage group spontaneous hypertension rat is starkly lower than control group, difference has conspicuousness (P < 0.05), and each sample has no significant effect the blood pressure of the heart rate of spontaneous hypertension rat, body weight and normal SD rats, heart rate and body weight, and they all have auxiliary function for lowering blood pressure to animal.

By present pre-ferred embodiments, spirit of the present invention is elaborated above.It will be appreciated by those skilled in the art that every above embodiment is done according to the technology of the present invention essence any amendment, equivalent variations and modification, all drop in protection scope of the present invention.

Claims

1. a composition, it is prepared by following material: rhizoma Gastrodiae, Folium Apocyni Veneti, folium cortex eucommiae, sophora flower, chrysanthemum, green tea.

2. composition according to claim 1, it is prepared by following material:

Or it is prepared by following material:

3., according to the composition of claim 1-2, it is the form in tea in bag.

4., according to the composition of claim 1-3, it is the form in tea in bag, and every bag of weight comprising rhizoma Gastrodiae is 0.1-0.3g, 0.15-0.25g or 0.2g.

5., according to the composition of claim 1-4, it is prepared by the method comprised the following steps:

6. prepare the method for composition, said composition is prepared by following material: rhizoma Gastrodiae, Folium Apocyni Veneti, folium cortex eucommiae, sophora flower, chrysanthemum, green tea; The method comprises the following steps:

7. method according to claim 6, wherein said composition is prepared by following material:

Or wherein said composition is prepared by following material:

8., according to the method for claim 6-7, wherein said composition is the form in tea in bag.

9., according to the method for claim 6-8, wherein said composition is the form in tea in bag, and every bag of weight comprising rhizoma Gastrodiae is 0.1-0.3g.

10., according to the method for claim 6-9, wherein said composition is the form in tea in bag, and every bag of weight comprising rhizoma Gastrodiae is 0.15-0.25g or 0.2g.