CN104585159A - Traditional Chinese medicine preservative for fish stripping specimen and preparation method of traditional Chinese medicine preservative - Google Patents
Traditional Chinese medicine preservative for fish stripping specimen and preparation method of traditional Chinese medicine preservative Download PDFInfo
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- CN104585159A CN104585159A CN201410842125.9A CN201410842125A CN104585159A CN 104585159 A CN104585159 A CN 104585159A CN 201410842125 A CN201410842125 A CN 201410842125A CN 104585159 A CN104585159 A CN 104585159A
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Abstract
The invention relates to a preservative, in particular to a traditional Chinese medicine preservative for a fish stripping specimen and a preparation method of the traditional Chinese medicine preservative. The method comprises the following steps: cleaning five traditional Chinese medicinal materials, namely rhubarb, coptis chinensis, astragalus membranaceus, falangal and fructus forsythiae; baking at 55-65 DEG C for 45-50 hours, and crushing until the granularity is below 40mesh; weighing all traditional Chinese medicines, putting the weighed traditional Chinese medicines into water, heating in a water bath at 55-65 DEG C for 10-14 hours, and then adding alums; or weighing all traditional Chinese medicines, putting the weighed traditional Chinese medicines into water, decocting into a paste with a slow fire, and then adding the alums; and adding p-nitrophenol to 95wt% ethyl alcohol, pouring the mixture into the medicinal materials in the step (2), stirring into a paste and sealing. The traditional Chinese medicine preservative for the fish stripping specimen has a good broad-spectrum bactericidal effect, is slow to release, nontoxic, healthy, and free of harm to bodies of preparation staff and users, and is capable of ensuring the preservative effect of the specimen for a long period of time; and the preparation method is simple and convenient, low in cost, relatively few in required equipment, and easy to popularize.
Description
Technical field
The present invention relates to a kind of preservative, particularly one is used for fish skinned-specimen Chinese medicine preservative and preparation method thereof.
Background technology
Aquatic animal skinned-specimen is teaching aid indispensable in bioconversion medium, has important viewing and admiring and scientific research value simultaneously.Its manufacturing process mainly comprises peeling, degreasing, anticorrosion, prosthese make, fill, sew up and the step such as colouring.Wherein the quality of antiseptic effect directly decides the service life of sample, and anticorrosion good sample can use last 100 years and anticorrosion bad sample will spoilage between several years.
The poisonous irritant smell preservatives such as traditional arsenic trioxide (arsenic), phenol and formaldehyde have certain toxicity, have both damaged preparation of specimen personnel, have not also met the theory of modern preparation of specimen and exhibition; Some preservative, as expensive in You Lan etc., be unfavorable for promoting and utilizing.And air comparatively humid region (coastal area) be difficult to guarantee good antiseptic effect, often there is part skinned-specimen that mould corruption occurs or the phenomenon and cause corrupted of damaging by worms, particularly some rare aquatic animals samples, as black finless porpoise, mandarin sturgeon, white pole globefish etc., just be difficult to obtain material once damage again make, waste resource and manpower and materials.
At present, existing nearly hundred kinds of natural traditional Chinese medicines are in the news and have good bacteriostasis, it is to human non-toxic's evil, can be applicable to the corrosion-resistant fields such as food, medicine and cosmetics, but essential oil, phenols, quinones, organic acid, alkaloid, polysaccharide, terpene and flavonoids etc. contained by it have the compound of fungistatic effect be difficult to purify utilize, if natural traditional Chinese medicine is applied to fishes snd anticorrosion in, both can reduce use cost, in turn ensure that making and user of service healthy, kill two birds with one stone.
Summary of the invention
The object of this invention is to provide one for fish skinned-specimen Chinese medicine preservative, this preservative is antibacterial effective ingredient with natural Chinese medicine, and antiseptic effect is excellent, and nontoxic, healthy, cost is low.
Another object of the present invention is to provide the above-mentioned using method for fish skinned-specimen Chinese medicine preservative.
Object of the present invention can be achieved through the following technical solutions:
A preparation method for fish skinned-specimen Chinese medicine preservative, its step comprises:
(1) by rheum officinale, the coptis, the Radix Astragali, galingal, the capsule of weeping forsythia totally five kinds of traditional Chinese medicines clean, under 55-65 DEG C of condition, toast 45-50 hour, and be crushed to below 40 orders; Preferably, by rheum officinale, the coptis, the Radix Astragali, galingal, the capsule of weeping forsythia totally five kinds of traditional Chinese medicines clean, under 60 DEG C of conditions toast 48 hours.
(2) water put into by all Chinese medicines got in step (1), and 55-65 DEG C added alum after heating water bath 10-14 hour; Or water put into by all Chinese medicines got in step (1), and little fire adds alum after being boiled into pasty state;
(3) at mass fraction be 95% alcohol in add p-nitrophenol, then pour in the medicinal material in step (2), stir into paste body shape, sealing.In whipping process, available a small amount of soap powder adjustment viscous pasty state.
In described step (1), the weight ratio of rheum officinale, the coptis, the Radix Astragali, galingal, the capsule of weeping forsythia is 1-4:1-3:1-2:1:1.
Chinese medicine gross weight in described step (2) and the weight ratio of water are 1:2-4.
In described step (2), the weight ratio of described rheum officinale and alum is 3-4:1.
The weight ratio of described Chinese medicine gross weight and alcohol is 1:1-3.
The weight ratio of described Chinese medicine gross weight and p-nitrophenol is 90-100:1.Suitable adds proportioning, is conducive to the fungistatic effect guaranteeing wide spectrum.
Prepared by above-mentioned preparation method, fish skinned-specimen Chinese medicine preservative can be obtained.
The using method of described fish skinned-specimen Chinese medicine preservative, the steps include:
(A) peel the fish-skin of specimen samples fish, then fish-skin is placed in the alcohol degreasing 4-7 days that mass concentration is 95%, take out fish-skin and be placed on clear water at immersion 1-2 hour;
(B) by fish skinned-specimen Chinese medicine preservative uniform application inside hide head and body, then hide is through on the prosthese made, sews up the incision, sample base is installed, arrange attitude, paint after drying.
In described step (B), the applying amount of fish skinned-specimen Chinese medicine preservative is the 5-10% of fish-skin weight.Different smears ratio, will directly affects the antiseptic effect of sample.
Compared with prior art, beneficial effect of the present invention is:
1, described fish skinned-specimen Chinese medicine preservative has good broad-spectrum sterilization effect, slow releasing, can ensure the antiseptic effect of sample for a long time.
2, the component of described Chinese medicine preservative is natural plants, nontoxic, healthy, to making and user of service without physical impairment, especially particularly important when school, Museum Personnel are comparatively intensive.
3, inorganic to essential oil various in natural traditional Chinese medicine, phenols, quinones, organic acid, alkaloid, polysaccharide, terpene and flavonoids etc. and organic effective component fully extract by this preparation method, good antimicrobial effect.
4, this preparation method is easy, with low cost, and equipment needed thereby is less, easier utilization and extention.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
(1) five kinds of Chinese medicines such as rheum officinale, the coptis, the Radix Astragali, galingal, the capsule of weeping forsythia are cleaned, be placed in baking oven (60 DEG C, 48 hours) respectively and dry, be broken into powder with cracker, 40 mesh sieve thin,tough silk can be crossed.
(2) weigh rheum officinale 350g, coptis 250g, Radix Astragali 150g, galingal 100g, capsule of weeping forsythia 100g respectively, add the warm water 2850ml of 60 DEG C, insert after stirring (60 DEG C, 12 hours) in water-bath; Or 90 DEG C of little fire of electric hot plate are brewed into pasty state, and stirring per minute once, adds alum 100g after cooling.
(3) 95% alcohol 1900ml is measured, 10g p-nitrophenol is dissolved in wherein, then alcoholic solution is poured in the medicinal material for subsequent use in step (2), stir, pour in closed container and extract 24 hours, obtained fish skinned-specimen Chinese medicine preservative.
Using method:
For grass carp sample, being peeled by the fish-skin of grass carp and being placed on mass concentration is degreasing 4-7 days in the alcohol of 95%, take out fish-skin, be placed in clear water to soak and ease back for 1-2 hour, the weight weighing fish-skin is 1360g, take fish skinned-specimen Chinese medicine preservative obtained in 68g the present embodiment, with brush uniform application inside hide head and body, then hide is through on the prosthese made, sew up the incision, sample base is installed, arrange attitude, coat color after treating its drying, so far grass carp preparation of specimen completes.
Embodiment 2
Weigh each 50g of rheum officinale, the coptis, the Radix Astragali, galingal, the capsule of weeping forsythia and alum respectively, above-mentioned Chinese medicine is placed in 60 DEG C of baking ovens 48 hours, be crushed to below 40 orders, be respectively charged into after weighing in triangular flask, respectively add 150ml water and be placed in water-bath (60 DEG C, 12 hours), add 95% alcohol 250ml again, sealing, 40 DEG C, 100rpm constant temperature vibration extracts 12 hours.Centrifugal 30 minutes of 3000r/min, becomes supernatant rotary evaporation containing crude drug 100% (W/V) concentration, seals 4 DEG C of preservations.
Prepared by nutrient broth medium, take beef extract 5g, sodium chloride 5g, peptone l0g, glucose l0g, add water to 1000ml, boil and each composition is fully dissolved, and after being cooled to normal temperature, adjustment pH is 7.6 ~ 7.8, and solid culture medium then adds agar 1.5-2%.For the cultivation of Escherichia coli, staphylococcus aureus and brewer's yeast.Prepared by potato culture, get peeled potatoes 300g, be cut into small pieces, add water boil 20 minutes, and cross leaching juice, add glucose 10g, add water to 1000ml, natural ph, solid culture medium then adds agar 1.5-2%, cultivates for aspergillus niger.The all sterilizings 20 minutes at 121 DEG C of temperature of above medium.
Escherichia coli, staphylococcus aureus, brewer's yeast and black-koji mould are washed down from culture dish by stroke-physiological saline solution, break up and obtain bacteria suspension, blood counting chamber counts, and the concentration of adjustment bacteria suspension is 10
5-10
6individual/mL.
By two-fold dilution's method, the extracts of Chinese herbal medicine of 1g/ml is diluted to ten concentration gradients such as 1g/ml to 1.95mg/m1, then l ml is drawn in the culture dish of sterilizing with Sterile pipette, add meat soup or the potato agar medium of 9ml thawing, fully mixing makes liquid ultimate density be 100mg/ml to 0.195mg/ml.After medium cooling, coat for examination bacterium liquid with transfer needle setting-out, cultivate 24 ~ 48 hours under 28 DEG C of constant temperature, observe the growing state of thalline, drug concentration corresponding to the culture dish of asepsis growth is the minimum inhibitory concentration (MIC) of preservative.With stroke-physiological saline solution in contrast, concrete fungistatic effect figure is as shown in table 1.
The inhibitory potency table of the single Chinese medicine of table 1 and formula
Inhibitory potency >=1/640 (1.56mg) of medicine during " +++ " dilution method
The inhibitory potency > 1/80 (12.5mg) of medicine during " ++ " dilution method
Inhibitory potency≤1/80 of medicine during "+" dilution method
"-" trial drug is without bacteriostasis
As can be seen from the above table, the capsule of weeping forsythia is stronger to bacillus, the inhibitory action of alum to yeast to mould, the coptis, all there is very strong inhibitory action to staphylococcus aureus, and the fish skinned-specimen Chinese medicine preservative in embodiment 1 shows certain coordinate repression, inhibitory potency is higher, and antimicrobial spectrum is wider.
The above is preferred embodiment of the present invention, but the present invention should not be confined to the content disclosed in this embodiment.The equivalence completed under not departing from spirit disclosed in this invention so every or amendment, all fall into the scope of protection of the invention.
Claims (9)
1. a preparation method for fish skinned-specimen Chinese medicine preservative, its step comprises:
(1) by rheum officinale, the coptis, the Radix Astragali, galingal, the capsule of weeping forsythia totally five kinds of traditional Chinese medicines clean, under 55-65 DEG C of condition, toast 45-50 hour, and be crushed to below 40 orders;
(2) water put into by all Chinese medicines taken in step (1), and 55-65 DEG C added alum after heating water bath 10-14 hour; Or water put into by the Chinese medicine got in step (1), and little fire adds alum after being boiled into pasty state;
(3) at mass fraction be 95% alcohol in add p-nitrophenol, then pour in the medicinal material in step (2), stir into paste body shape, sealing.
2. the preparation method of fish skinned-specimen Chinese medicine preservative according to claim 1, is characterized in that: in described step (1), and the weight ratio of rheum officinale, the coptis, the Radix Astragali, galingal, the capsule of weeping forsythia is 1-4:1-3:1-2:1:1.
3. the preparation method of fish skinned-specimen Chinese medicine preservative according to claim 1, is characterized in that: the Chinese medicine gross weight in described step (2) and the weight ratio of water are 1:2-4.
4. the preparation method of fish skinned-specimen Chinese medicine preservative according to claim 1, is characterized in that: in described step (2), and the weight ratio of described rheum officinale and alum is 3-4:1.
5. the preparation method of fish skinned-specimen Chinese medicine preservative according to claim 1, is characterized in that: the weight ratio of described Chinese medicine gross weight and alcohol is 1:1-3.
6. the preparation method of fish skinned-specimen Chinese medicine preservative according to claim 1, is characterized in that: the weight ratio of described Chinese medicine gross weight and p-nitrophenol is 90-100:1.
7. a fish skinned-specimen Chinese medicine preservative, is characterized in that, by the method preparation described in any one in claim 1-6.
8. the using method of fish skinned-specimen Chinese medicine preservative according to claim 7, the steps include:
(A) peel the fish-skin of sample fish, then fish-skin is placed in the alcohol degreasing 4-7 days that mass fraction is 95%, taking-up fish-skin is placed in clear water and soaks 1-2 hour;
(B) by fish skinned-specimen Chinese medicine preservative uniform application inside hide head and body, then hide is through on the prosthese made, sews up the incision, sample base is installed, arrange attitude, paint after drying.
9. the using method of fish skinned-specimen Chinese medicine preservative according to claim 8, is characterized in that: in described step (B), and the applying amount of fish skinned-specimen Chinese medicine preservative is the 5-10% of fish-skin weight.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107578687A (en) * | 2017-09-21 | 2018-01-12 | 上海海洋大学 | A kind of novel fish decorticating prosthese Slide processing |
| CN108902643A (en) * | 2018-06-29 | 2018-11-30 | 苏州汉德瑞生物工程有限公司 | A kind of biological food preservative and preparation method thereof |
| CN111226903A (en) * | 2020-03-05 | 2020-06-05 | 任素丽 | Chinese herbal medicine preservative for animal specimens |
| CN111771867A (en) * | 2020-07-13 | 2020-10-16 | 许昌学院 | Preservative for biological dissection experiments and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601377A (en) * | 2008-06-12 | 2009-12-16 | 上海遗体防腐研究所 | Medium-term and long-term antiseptic preserving fluid of remains and application thereof |
| CN102067892A (en) * | 2010-12-15 | 2011-05-25 | 华南农业大学 | Anticorrosive Chinese medicine preparation and application thereof |
| CN102614440A (en) * | 2012-04-27 | 2012-08-01 | 伊彩虹 | Multi-herb antibacterial liquid |
-
2014
- 2014-12-25 CN CN201410842125.9A patent/CN104585159B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601377A (en) * | 2008-06-12 | 2009-12-16 | 上海遗体防腐研究所 | Medium-term and long-term antiseptic preserving fluid of remains and application thereof |
| CN102067892A (en) * | 2010-12-15 | 2011-05-25 | 华南农业大学 | Anticorrosive Chinese medicine preparation and application thereof |
| CN102614440A (en) * | 2012-04-27 | 2012-08-01 | 伊彩虹 | Multi-herb antibacterial liquid |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107578687A (en) * | 2017-09-21 | 2018-01-12 | 上海海洋大学 | A kind of novel fish decorticating prosthese Slide processing |
| CN108902643A (en) * | 2018-06-29 | 2018-11-30 | 苏州汉德瑞生物工程有限公司 | A kind of biological food preservative and preparation method thereof |
| CN111226903A (en) * | 2020-03-05 | 2020-06-05 | 任素丽 | Chinese herbal medicine preservative for animal specimens |
| CN111771867A (en) * | 2020-07-13 | 2020-10-16 | 许昌学院 | Preservative for biological dissection experiments and preparation method thereof |
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| CN104585159B (en) | 2017-02-22 |
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Inventor after: Zhou Tianshu Inventor after: Zhang Ya Inventor after: Tang Wenqiao Inventor after: Teng Yilan Inventor after: Tong Hongyang Inventor after: Bao Yanlin Inventor after: Xu Can Inventor before: Zhang Ya Inventor before: Zhou Tianshu Inventor before: Tang Wenqiao Inventor before: Teng Yilan Inventor before: Tong Hongyang Inventor before: Bao Yanlin Inventor before: Xu Can |
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Granted publication date: 20170222 Termination date: 20171225 |