CN111202103A - Plant bactericide and preparation method thereof - Google Patents
Plant bactericide and preparation method thereof Download PDFInfo
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- CN111202103A CN111202103A CN202010159612.0A CN202010159612A CN111202103A CN 111202103 A CN111202103 A CN 111202103A CN 202010159612 A CN202010159612 A CN 202010159612A CN 111202103 A CN111202103 A CN 111202103A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
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Abstract
The invention relates to the technical field of cosmetics, in particular to a plant bactericide and a preparation method thereof. In order to solve the above technical problems, a first aspect of the present invention provides a method for preparing a plant bactericide, comprising the steps of: (1) pulverizing the plant materials, and sieving to obtain mixture; (2) adding 95% ethanol 5-10 times the weight of the mixture into the mixture, extracting at 60-70 deg.C for 1-3 hr, and filtering to obtain filtrate A; (3) repeatedly extracting the rest residue with 95% ethanol 0.5-3 times the weight of the residue for 2-3 times to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/(10-20) of the total volume to obtain plant extract; (4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a plant bactericide and a preparation method thereof.
Background
Biocides, also known as biocides, bactericidal algicides, microbicides, and the like, generally refer to chemical agents that are effective in controlling or killing microorganisms, bacteria, fungi, and algae, in aqueous systems. Internationally, it is a general term for agents for controlling various types of pathogenic microorganisms. Mainly divided into agricultural bactericide and industrial bactericide. Among them, agricultural fungicides are a class of agricultural chemicals for controlling plant diseases caused by various pathogenic microorganisms, and generally refer to fungicides. Along with the development of bactericides, virucides, algicides and other subclasses are distinguished.
At present, agricultural bactericides are mainly chemical bactericides, and when a large amount of chemical bactericides are used, the problems of chemical drug residues, environmental pollution, influence on human health and the like easily exist, and meanwhile, the pesticide resistance is easily generated, so that the use of chemical bactericides needs to be reduced to avoid the problems.
Disclosure of Invention
In order to solve the above technical problems, a first aspect of the present invention provides a method for preparing a plant bactericide, comprising the steps of:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 5-10 times the weight of the mixture into the mixture, extracting at 60-70 deg.C for 1-3 hr, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 95% ethanol 0.5-3 times the weight of the residue for 2-3 times to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/(10-20) of the total volume to obtain plant extract;
(4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
As a preferable technical scheme, the plant raw materials are selected from asarum, Chinese pulsatilla root, common andrographis herb, rhubarb, garlic, officinal magnolia bark, common sage root, liquoric root, lesser paris rhizome, weeping forsythia capsule, safflower purslane, red-leaf chenopodium herb, fineleaf schizonepeta herb, onion, hairyvein agrimony and Chinese lobelia herb.
As a preferred technical scheme, the plant raw materials comprise asarum, Chinese pulsatilla root, common andrographis herb, officinal magnolia bark, weeping forsythia, red flower purslane, red-leaf chenopodium, hairyvein agrimony and Chinese lobelia herb.
As a preferred technical scheme, the plant raw materials comprise, by weight, 1-5 parts of asarum, 1-5 parts of Chinese pulsatilla root, 1-5 parts of common andrographis herb, 1-5 parts of officinal magnolia bark, 1-3 parts of weeping forsythia capsule, 1-3 parts of safflower purslane, 1-3 parts of red-leaf chenopodium album, 1-3 parts of hairyvein agrimony and 1-3 parts of Chinese lobelia.
As a preferred technical scheme, the plant raw materials comprise, by weight, 2-5 parts of asarum, 2-5 parts of Chinese pulsatilla root, 1-4 parts of common andrographis herb, 1-4 parts of officinal magnolia bark, 1-3 parts of weeping forsythia capsule, 1-3 parts of safflower purslane, 1-2 parts of red-leaf chenopodium album, 1-2 parts of hairyvein agrimony and 1-2 parts of Chinese lobelia.
As a preferred technical scheme, the volume ratio of the plant extract to the ginger and sophora flavescens extracting solution in the step (4) is 1: (1-1.2).
As a preferred technical scheme, the volume ratio of the plant extract to the ginger and sophora flavescens extracting solution in the step (4) is 1: 1.
in a second aspect, the invention provides a plant bactericide, which is prepared according to the preparation method.
As a preferable technical scheme, the form of the bactericide comprises oil, emulsifier, aqueous solvent, aerosol, spray, suspending agent, granule, powder, microgranule, capsule, drop, fumigant, aerosol and smoke curtain agent.
The third aspect of the invention provides an application of the plant bactericide, which is used in the fields of foods, cosmetics, personal cleaning products, household cleaning products and daily chemical products.
Has the advantages that: the invention adopts the all-natural plant extract, and adopts the special extraction process to obtain the natural plant raw materials, thereby achieving better synergistic effects of sterilization, antibiosis and bacteriostasis; the raw materials of the product are all taken from natural plants, chemical additives are not contained, the product is safe, no side effect is caused, the product and the by-products of the product are nontoxic and harmless to human bodies, and the environmental pollution is avoided, so that the product can be widely applied to the fields of food, cosmetics, personal cleaning products, household cleaning products, daily chemical products and the like, and different applications of environmental disinfection, equipment disinfection, product anticorrosion and bacteria prevention in the daily chemical industry are realized.
Detailed Description
Unless otherwise indicated, implied from the context, or customary in the art, all parts and percentages herein are by weight and the testing and characterization methods used are synchronized with the filing date of the present application. To the extent that a definition of a particular term disclosed in the prior art is inconsistent with any definitions provided herein, the definition of the term provided herein controls.
The technical features of the technical solutions provided by the present invention are further clearly and completely described below with reference to the specific embodiments, and the scope of protection is not limited thereto.
The words "preferred", "preferably", "more preferred", and the like, in the present invention, refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention. The sources of components not mentioned in the present invention are all commercially available.
In order to solve the above technical problems, a first aspect of the present invention provides a method for preparing a plant bactericide, comprising the steps of:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 5-10 times the weight of the mixture into the mixture, extracting at 60-70 deg.C for 1-3 hr, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 1-3 times of 95% ethanol to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/(10-20) of the total volume to obtain plant extract;
(4) adding water, ginger and radix sophorae flavescentis extract and anti-allergy agent into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
As a preferable technical scheme, the plant raw materials are selected from asarum, Chinese pulsatilla root, common andrographis herb, rhubarb, garlic, officinal magnolia bark, common sage root, liquoric root, lesser paris rhizome, weeping forsythia capsule, safflower purslane, red-leaf chenopodium herb, fineleaf schizonepeta herb, onion, hairyvein agrimony and Chinese lobelia herb.
As a preferred technical scheme, the plant raw materials comprise asarum, Chinese pulsatilla root, common andrographis herb, officinal magnolia bark, weeping forsythia, red flower purslane, red-leaf chenopodium, hairyvein agrimony and Chinese lobelia herb.
As a preferred technical scheme, the plant raw materials comprise, by weight, 1-5 parts of asarum, 1-5 parts of Chinese pulsatilla root, 1-5 parts of common andrographis herb, 1-5 parts of officinal magnolia bark, 1-3 parts of weeping forsythia capsule, 1-3 parts of safflower purslane, 1-3 parts of red-leaf chenopodium album, 1-3 parts of hairyvein agrimony and 1-3 parts of Chinese lobelia.
As a preferred technical scheme, the plant raw materials comprise, by weight, 2-5 parts of asarum, 2-5 parts of Chinese pulsatilla root, 1-4 parts of common andrographis herb, 1-4 parts of officinal magnolia bark, 1-3 parts of weeping forsythia capsule, 1-3 parts of safflower purslane, 1-2 parts of red-leaf chenopodium album, 1-2 parts of hairyvein agrimony and 1-2 parts of Chinese lobelia.
As a preferred technical scheme, the plant raw materials comprise, by weight, 3.5 parts of asarum, 4 parts of Chinese pulsatilla root, 2 parts of common andrographis herb, 2 parts of officinal magnolia bark, 2 parts of weeping forsythia, 2 parts of safflower purslane, 1.5 parts of red-leaf chenopodium, 1.5 parts of hairyvein agrimony and 1.5 parts of Chinese lobelia.
The asarum is named as the traditional Chinese medicine. Also named: asarum sieboldii, asarum xiao, asarum simsii, asarum schreberi, etc., belonging to the order Aristolochiales, the family Aristolochiaceae, the family of perennial herbs; the rootstock is upright or transverse, and has a plurality of fibrous roots; 2 leaves are usually in heart shape or egg shape, and the flower is purple black; the flower stalk is 2-100 px long; the flower envelope has a triangular, oval shape, approximately spherical shape, a diameter of about 37.5px, and a brownish yellow color. The flowering period is 4-5 months. Has the functions of expelling wind, dispelling cold, promoting diuresis, inducing resuscitation, etc. It is commonly used for wind-cold headache, nasosinusitis, toothache, phlegm-fluid cough, wind-damp arthralgia, etc. The asarum can not only externally disperse wind-cold but also internally dispel yin-cold, and has better effects of relieving pain and cough.
The white head Bulbul (with the scientific name of Pycnontus sinensis, English name of Light-proved Bulbul or Chinese Bulbul), Chinese pulsatilla and Chinese larval shell (Tai language) is a sparrow-shaped bird of 40526and the small birds of the family are birds, namely horns, south of northern birds in winter are migratory birds, Taiwan subspecies are reserved birds in Taiwan of China, and the average life is about 10 to 15 years. Pulsatillae radix is commonly found in east asia, yilan plains of taiwan, and mainland china is in most regions of south of the Yangtze river, hong kong, yuzuo islands (yayao mountain islands, ropes, etc.), and is lively and not very fearful. Eating insects, seeds and fruits, belonging to the omnivorous property, the breasts of the male birds are darker in gray, the females are lighter in gray, and the occiput (back heads) of the male birds are extremely clear and striking in white.
The Andrographis paniculata Nees is called Andrographis paniculata (Burm.f.) Nees, also called Chunlianqiliu, Yijianxi, Sclerian bluestem, scabrous elephantfoot herb, herba lysimachiae foenum-graecum, radix Stephaniae Tetrandrae, India grass, bitter grass and the like. Annual herbaceous plants, 4-8 cm long, 1-2.5 cm wide. The medicinal plants have the functions of clearing away heat and toxic materials, diminishing inflammation, and relieving swelling and pain.
The Magnolia officinalis is also called Magnolia officinalis, Magnolia officinalis and the like in the botanical range, is a Magnolia plant and a Magnolia plant, is commonly called Magnolia officinalis (original subspecies) Magnolia officinalis of Magnolia officinalis subspecies of Magnolia officinalis of subspecies of Magnolia officinalis of subspecies. The Chinese medicinal materials refer to dried bark, root bark and branch bark of the plant. Stripping off root bark and branch bark for 4-6 months, directly drying in the shade, slightly boiling the dried bark in boiling water, piling up the dried bark in a shady and humid place, steaming and softening the dried bark until the inner surface of the dried bark is purple brown or tan, taking out the dried bark, rolling the dried bark into a cylinder shape, and drying. Cut into shreds and prepare ginger. Can be used for treating dyspepsia, abdominal distention, constipation, and damp obstruction of middle warmer.
The Forsythia (Latin name: Forsythia subspensa): is deciduous shrub of Forsythia of Oleaceae of twisted order of dicotyledonae. The forsythia is bloomed firstly in early spring, the flower blooms with light and bright fragrance, the full branches are golden and gorgeous, the forsythia is an excellent flower-viewing shrub in early spring, the plant height can reach 3 meters, the branches and the trunks grow, the small branches are yellow, the forsythia is arched and sagged, and the forsythia is hollow. Folio, unilobal or trifoliate, oval or ovoid, with toothed edges. The corolla is yellow, and 1-3 flowers grow in the axilla of the leaf; the fruit egg is spherical, oval or oblong, the shape of the end beak is gradually tapered, and the surface is provided with skin holes; the fruit stalks are 0.7-1.5 cm long. The flowering period is 3-4 months, and the fruit period is 7-9 months.
The purslane (scientific name: Portulaca grandiflora Hook.): annual herbaceous plants, 10-30 cm high. Horizontal or oblique stem, purple red, multiple branches, and clumpy hairs on the nodes. The leaves are dense at the branch ends, the lower leaves are separated, the leaves are irregularly grown, the leaves are thin and cylindrical, and the leaves are hairless. The diameter of the branch end of the single or a plurality of clusters is 2.5-4 cm, and the branch end is closed after the day is opened; the involucre is 8-9 pieces, leaf-shaped and recurrent, and has white long and soft hair; petal 5 or heavy petal, inverted egg shape, slightly concave top, 12-30 mm long, red, purple or yellow-white. The capsule is nearly elliptical and is cracked; the seeds are small and most of them are round kidney-shaped, and the diameter is less than 1 mm. The flowering period is 6-9 months, and the fruit period is 8-11 months.
The described red-leaf chenopodium quinoa is mainly distributed in the subclass, middle Asia, Europe, North America and Chinese mainland, and belongs to the annual herbaceous plant of angiosperma, dicotyledonous plant, Caryophyllales and Amaranthaceae, its height is 30-80 cm, stem is upright or obliquely raised, and is light green or reddish, and has strip edge but has no obvious colour strip, and its upper portion is equipped with branch whose length is 2-8 cm.
The agrimony is named as the traditional Chinese medicine. Is dried aerial part of Agrimonia pilosa Ledeb of Rosaceae. The lower part of the stem is cylindrical, the diameter of the stem is 4-6 mm, the stem is reddish brown, the upper part of the stem is square cylindrical, four sides of the stem are slightly concave, the stem is greenish brown, and the stem is provided with longitudinal furrows, ridge lines and nodes; light weight, hard quality, easy breaking and hollow section.
The herba Scutellariae Barbatae, Portulacaceae, annual herbaceous plant. Wide distribution and preference for dampness. The whole herb can be used as a medicine, and has the effects of cooling blood, removing toxicity, reducing swelling, clearing heat and promoting diuresis. The whole herb is used as a medicine for clearing heat, detoxifying, dissipating guilt and relieving pain. It is used to treat hematemesis, hemoptysis, dysentery, cellulitis, pharyngalgia, pulmonary abscess, skin ulcer, pestilence, and snake bite.
In some embodiments of the present invention, the volume ratio of the plant extract to the ginger and sophora flavescens extract in the step (4) is 1: (1-1.2).
In some embodiments of the present invention, the volume ratio of the plant extract to the ginger and sophora flavescens extract in the step (4) is 1: 1.
in a preferred embodiment of the present invention, the ginger and sophora flavescens extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens, and extracting with water or diluted alcohol under reflux; then filtering or centrifuging to obtain a clear solution; then adding beta-cyclodextrin, and stirring at 40-70 deg.C for 30-60 min; adding pulverized radix Sophorae Flavescentis, extracting with water or diluted alcohol at 50-80 deg.C for 1-2 hr, ultrafiltering the extractive solution with 3K filter membrane, collecting the filtrate, and adjusting pH to 4-5 to obtain rhizoma Zingiberis recens-radix Sophorae Flavescentis extractive solution.
The inventors have found that when diluted alcohol solutions of different concentrations are used, the ginger and sophora flavescens extract obtained under the preferred conditions of the present invention has an excellent bactericidal effect.
In a preferred embodiment of the present invention, the mass ratio of ginger to sophora flavescens is (2-20): 1; preferably, the mass ratio of the ginger to the radix sophorae flavescentis is (2-10): 1; more preferably, the mass ratio of the ginger to the sophora flavescens is 7.5: 1.
in a preferred embodiment of the invention, the mass ratio of ginger to beta-cyclodextrin is (10-30): 1; more preferably, the mass ratio of the ginger to the beta-cyclodextrin is (15-30): 1; more preferably, the mass ratio of the ginger to the beta-cyclodextrin is 22: 1.
the cyclodextrin is added in the preparation steps of the invention, part of gingerol can be included, the stability of the antibacterial component gingerol is kept, but if the cyclodextrin is mixed with sophora flavescens and the like for use, the extraction temperature is increased, the diluted alcohol is kept to extract according to gradient concentration, the neutralization of the diluted alcohol and the matrine is promoted, and water-soluble salt with relatively large molecular mass is generated, so that the inventor unexpectedly finds that when ginger and sophora flavescens are combined, the sterilization effect is excellent, the water-soluble salt can be generated based on the generation of the diluted alcohol, and then the pH is adjusted to 4-5, which is beneficial to the stability of gingerol.
Specifically, in a most preferred embodiment of the present invention, the ginger and sophora flavescens extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens into 20-60 mesh, and reflux-extracting with 1-2 times of 30-40% ethanol; then filtering or centrifuging to obtain a clear solution; then adding beta-cyclodextrin, and stirring at 40-70 deg.C for 30-60 min; adding pulverized radix Sophorae Flavescentis of 20-60 meshes, and extracting with 30-40% ethanol 1-2 times of radix Sophorae Flavescentis powder at 50-80 deg.C under reflux for 30-60 min; adding 0.5-1 times of 30-50% ethanol, and reflux extracting for 30-60 min; adding a small amount of water after extraction, performing ultrafiltration on the extract by using a 3K filter membrane, collecting the permeate, and adjusting the pH value to 4-5 to obtain a ginger and radix sophorae flavescentis extract; the mass ratio of the ginger to the radix sophorae flavescentis is (2-10): 1; the mass ratio of the ginger to the beta-cyclodextrin is (15-30): 1; the mass ratio of the water to the ginger powder is 1: (4-10).
More specifically, in the most preferred embodiment of the present invention, the ginger and sophora flavescens extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens into 40 mesh, and extracting with 2 times of 35% ethanol under reflux; centrifuging at 8000r/min for 15min to obtain clear solution; then adding beta-cyclodextrin into the mixture, and stirring the mixture for 30min at the temperature of 60 ℃; adding pulverized radix Sophorae Flavescentis of 40 meshes, and extracting with 35% ethanol 2 times of radix Sophorae Flavescentis powder at 70 deg.C under reflux for 30 min; adding 40% ethanol 0.5 times the weight of radix Sophorae Flavescentis powder, and reflux extracting for 60 min; adding a small amount of water, stirring, ultrafiltering the extractive solution with 3K filter membrane, collecting the filtrate, and adjusting pH to 4-5 to obtain rhizoma Zingiberis recens-radix Sophorae Flavescentis extractive solution; the mass ratio of the ginger to the radix sophorae flavescentis is 7.5: 1; the mass ratio of the ginger to the beta-cyclodextrin is 22: 1; the mass ratio of the water to the ginger powder is 1: 5.
in a second aspect, the invention provides a plant bactericide, which is prepared according to the preparation method.
The form of the bactericide comprises oil, emulsifier, aqueous solvent, aerosol, spray, suspending agent, granule, powder, microgranule, capsule, drop, fumigant, aerosol and smoke agent.
The third aspect of the invention provides an application of the plant bactericide, which is used in the fields of foods, cosmetics, personal cleaning products, household cleaning products and daily chemical products.
The present invention is described in detail below with reference to examples, which are provided for the purpose of further illustration only and are not to be construed as limiting the scope of the present invention, and the insubstantial modifications and adaptations thereof by those skilled in the art based on the teachings of the present invention will still fall within the scope of the present invention.
Example 1
Embodiment 1 provides a method for preparing a plant bactericide, comprising the steps of:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 5 times the weight of the mixture into the mixture, extracting at 65 deg.C for 2 hr, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 2 times of 95% ethanol for 3 times to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/15 to obtain plant extract;
(4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
The plant raw materials comprise, by weight, 2 parts of asarum, 2 parts of Chinese pulsatilla root, 4 parts of common andrographis herb, 4 parts of officinal magnolia bark, 1 part of weeping forsythia, 1 part of safflower purslane, 2 parts of red-leaf chenopodium album, 2 parts of hairyvein agrimony and 1 part of Chinese lobelia;
the volume ratio of the plant extract to the ginger and radix sophorae flavescentis extract in the step (4) is 1: 1;
the ginger and radix sophorae flavescentis extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens into 20 mesh, and extracting with 2 times of 35% ethanol under reflux; centrifuging at 8000r/min for 15min to obtain clear solution; then adding beta-cyclodextrin, and stirring at 60 ℃ for 60 min; adding pulverized radix Sophorae Flavescentis of 20 meshes, and extracting with 35% ethanol 2 times of radix Sophorae Flavescentis powder at 70 deg.C under reflux for 60 min; adding 40% ethanol 0.5 times the weight of radix Sophorae Flavescentis powder, and reflux extracting for 60 min; adding a small amount of water after extraction, performing ultrafiltration on the extract by using a 3K filter membrane, collecting the permeate, and adjusting the pH value to 4-5 to obtain a ginger and radix sophorae flavescentis extract; the mass ratio of the ginger to the radix sophorae flavescentis is 4: 1; the mass ratio of the ginger to the beta-cyclodextrin is 15: 1; the mass ratio of the water to the ginger powder is 1: 4.
example 2
Embodiment 2 provides a method for preparing a plant bactericide, comprising the steps of:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 10 times the weight of the mixture into the mixture, extracting at 65 deg.C for 1h, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 2 times of 95% ethanol for 3 times to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/15 to obtain plant extract;
(4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
The plant raw materials comprise, by weight, 5 parts of asarum, 5 parts of Chinese pulsatilla root, 1 part of common andrographis herb, 1 part of officinal magnolia bark, 3 parts of weeping forsythia, 3 parts of safflower purslane, 1 part of red chenopodium album, 1 part of hairyvein agrimony and 2 parts of Chinese lobelia;
the volume ratio of the plant extract to the ginger and radix sophorae flavescentis extract in the step (4) is 1: 1.2;
the ginger and radix sophorae flavescentis extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens into 60 mesh, and extracting with 2 times of 35% ethanol under reflux; centrifuging at 8000r/min for 15min to obtain clear solution; then adding beta-cyclodextrin into the mixture, and stirring the mixture for 30min at the temperature of 60 ℃; adding pulverized 60-mesh radix Sophorae Flavescentis, and extracting with 35% ethanol at 70 deg.C for 3 min; adding 40% ethanol 1 times of radix Sophorae Flavescentis powder, and reflux extracting for 30 min; adding a small amount of water after extraction, performing ultrafiltration on the extract by using a 3K filter membrane, collecting the permeate, and adjusting the pH value to 4-5 to obtain a ginger and radix sophorae flavescentis extract; the mass ratio of the ginger to the radix sophorae flavescentis is 5: 1; the mass ratio of the ginger to the beta-cyclodextrin is 30: 1; the mass ratio of the water to the ginger powder is 1: 6.
example 3
Embodiment 3 provides a method for preparing a plant bactericide, comprising the steps of:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 5 times the weight of the mixture into the mixture, extracting at 65 deg.C for 2 hr, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 2 times of 95% ethanol for 3 times to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/15 to obtain plant extract;
(4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
The plant raw materials comprise, by weight, 3.5 parts of asarum, 4 parts of Chinese pulsatilla root, 2 parts of common andrographis herb, 2 parts of officinal magnolia bark, 2 parts of weeping forsythia, 2 parts of safflower purslane, 1.5 parts of red-leaf chenopodium album, 1.5 parts of hairyvein agrimony and 1.5 parts of Chinese lobelia herb;
the volume ratio of the plant extract to the ginger and radix sophorae flavescentis extract in the step (4) is 1: 1;
the ginger and radix sophorae flavescentis extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens into 40 mesh, and extracting with 2 times of 35% ethanol under reflux; centrifuging at 8000r/min for 15min to obtain clear solution; then adding beta-cyclodextrin into the mixture, and stirring the mixture for 30min at the temperature of 60 ℃; adding pulverized radix Sophorae Flavescentis of 40 meshes, and extracting with 35% ethanol 2 times of radix Sophorae Flavescentis powder at 70 deg.C under reflux for 30 min; adding 40% ethanol 0.5 times the weight of radix Sophorae Flavescentis powder, and reflux extracting for 60 min; adding a small amount of water, stirring, ultrafiltering the extractive solution with 3K filter membrane, collecting the filtrate, and adjusting pH to 4-5 to obtain rhizoma Zingiberis recens-radix Sophorae Flavescentis extractive solution; the mass ratio of the ginger to the radix sophorae flavescentis is 7.5: 1; the mass ratio of the ginger to the beta-cyclodextrin is 22: 1; the mass ratio of the water to the ginger powder is 1: 5.
example 4
Embodiment 4 provides a method for preparing a plant bactericide, comprising the steps of:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 5 times the weight of the mixture into the mixture, extracting at 65 deg.C for 2 hr, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 2 times of 95% ethanol for 3 times to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/15 to obtain plant extract;
(4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
The plant raw materials comprise, by weight, 3.5 parts of asarum, 4 parts of Chinese pulsatilla root, 2 parts of common andrographis herb, 2 parts of officinal magnolia bark, 2 parts of weeping forsythia, 2 parts of safflower purslane, 1.5 parts of red-leaf chenopodium album, 1.5 parts of hairyvein agrimony and 1.5 parts of Chinese lobelia herb;
the volume ratio of the plant extract to the ginger and radix sophorae flavescentis extract in the step (4) is 1: 1;
the ginger and radix sophorae flavescentis extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens into 40 mesh, and extracting with 2 times of 35% ethanol under reflux; centrifuging at 8000r/min for 15min to obtain clear solution; then adding beta-cyclodextrin into the mixture, and stirring the mixture for 30min at the temperature of 60 ℃; adding pulverized radix Sophorae Flavescentis of 40 meshes, and extracting with 35% ethanol 2 times of radix Sophorae Flavescentis powder at 60 deg.C under reflux for 90 min; adding a small amount of water, stirring, ultrafiltering the extractive solution with 3K filter membrane, collecting the filtrate, and adjusting pH to 4-5 to obtain rhizoma Zingiberis recens-radix Sophorae Flavescentis extractive solution; the mass ratio of the ginger to the radix sophorae flavescentis is 7.5: 1; the mass ratio of the ginger to the beta-cyclodextrin is 22: 1; the mass ratio of the water to the ginger powder is 1: 5.
example 5
Embodiment 5 provides a method for preparing a plant fungicide, comprising the steps of:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 5 times the weight of the mixture into the mixture, extracting at 65 deg.C for 2 hr, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 2 times of 95% ethanol for 3 times to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/15 to obtain plant extract;
(4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
The plant raw materials comprise, by weight, 3.5 parts of asarum, 4 parts of Chinese pulsatilla root, 2 parts of common andrographis herb, 2 parts of officinal magnolia bark, 2 parts of weeping forsythia, 2 parts of safflower purslane, 1.5 parts of red-leaf chenopodium album, 1.5 parts of hairyvein agrimony and 1.5 parts of Chinese lobelia herb;
the volume ratio of the plant extract to the ginger and radix sophorae flavescentis extract in the step (4) is 1: 1;
the ginger and radix sophorae flavescentis extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens into 40 mesh, and extracting with 2 times of 35% ethanol under reflux; centrifuging at 8000r/min for 15min to obtain clear solution; then adding beta-cyclodextrin into the mixture, and stirring the mixture for 30min at the temperature of 60 ℃; adding pulverized radix Sophorae Flavescentis of 40 meshes, and extracting with 35% ethanol 2 times of radix Sophorae Flavescentis powder at 70 deg.C under reflux for 30 min; adding 40% ethanol 0.5 times the weight of radix Sophorae Flavescentis powder, and reflux extracting for 60 min; adding a small amount of water, stirring, ultrafiltering the extractive solution with 3K filter membrane, collecting the filtrate, and adjusting pH to 4-5 to obtain rhizoma Zingiberis recens-radix Sophorae Flavescentis extractive solution; the mass ratio of the ginger to the radix sophorae flavescentis is 50: 1; the mass ratio of the ginger to the beta-cyclodextrin is 22: 1; the mass ratio of the water to the ginger powder is 1: 5.
example 6
Embodiment 6 provides a method for preparing a plant fungicide, comprising the steps of:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 5 times the weight of the mixture into the mixture, extracting at 65 deg.C for 2 hr, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 2 times of 95% ethanol for 3 times to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/15 to obtain plant extract;
(4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
The plant raw materials comprise, by weight, 3.5 parts of asarum, 4 parts of Chinese pulsatilla root, 2 parts of common andrographis herb, 2 parts of officinal magnolia bark, 2 parts of weeping forsythia, 2 parts of safflower purslane, 1.5 parts of red-leaf chenopodium album, 1.5 parts of hairyvein agrimony and 1.5 parts of Chinese lobelia herb;
the volume ratio of the plant extract to the ginger and radix sophorae flavescentis extract in the step (4) is 1: 1;
the ginger and radix sophorae flavescentis extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens into 40 mesh, and extracting with 2 times of 35% ethanol under reflux; centrifuging at 8000r/min for 15min to obtain clear solution; then adding beta-cyclodextrin into the mixture, and stirring the mixture for 30min at the temperature of 60 ℃; adding pulverized radix Sophorae Flavescentis of 40 meshes, and extracting with 35% ethanol 2 times of radix Sophorae Flavescentis powder at 70 deg.C under reflux for 30 min; adding 40% ethanol 0.5 times the weight of radix Sophorae Flavescentis powder, and reflux extracting for 60 min; adding a small amount of water, stirring, ultrafiltering the extractive solution with 3K filter membrane, collecting the filtrate, and adjusting pH to 4-5 to obtain rhizoma Zingiberis recens-radix Sophorae Flavescentis extractive solution; the mass ratio of the ginger to the radix sophorae flavescentis is 0.1: 1; the mass ratio of the ginger to the beta-cyclodextrin is 22: 1; the mass ratio of the water to the ginger powder is 1: 5.
example 7
Embodiment 7 provides a method for preparing a plant fungicide, comprising the steps of:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 5 times the weight of the mixture into the mixture, extracting at 65 deg.C for 2 hr, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 2 times of 95% ethanol for 3 times to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/15 to obtain plant extract;
(4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
The plant raw materials comprise, by weight, 3.5 parts of asarum, 4 parts of Chinese pulsatilla root, 2 parts of common andrographis herb, 2 parts of officinal magnolia bark, 2 parts of weeping forsythia, 2 parts of safflower purslane, 1.5 parts of red-leaf chenopodium album, 1.5 parts of hairyvein agrimony and 1.5 parts of Chinese lobelia herb;
the volume ratio of the plant extract to the ginger and radix sophorae flavescentis extract in the step (4) is 1: 1;
the ginger and radix sophorae flavescentis extracting solution is prepared by the following method:
pulverizing rhizoma Zingiberis recens into 40 mesh, and extracting with 2 times of 35% ethanol under reflux; centrifuging at 8000r/min for 15min to obtain clear solution; then adding beta-cyclodextrin into the mixture, and stirring the mixture for 30min at the temperature of 60 ℃; adding pulverized radix Sophorae Flavescentis of 40 meshes, and extracting with 35% ethanol 2 times of radix Sophorae Flavescentis powder at 70 deg.C under reflux for 30 min; adding 40% ethanol 0.5 times the weight of radix Sophorae Flavescentis powder, and reflux extracting for 60 min; adding a small amount of water, stirring, ultrafiltering the extractive solution with 3K filter membrane, collecting the filtrate, and adjusting pH to 4-5 to obtain rhizoma Zingiberis recens-radix Sophorae Flavescentis extractive solution; the mass ratio of the ginger to the radix sophorae flavescentis is 7.5: 1; the mass ratio of the ginger to the beta-cyclodextrin is 50: 1; the mass ratio of the water to the ginger powder is 1: 5.
performance testing
1. Stability test
The test method comprises standing the bactericide of the example at room temperature for 18 months, observing whether the bactericide solution has precipitation and discoloration, and if the bactericide solution has precipitation or discoloration, the bactericide solution fails to be qualified, and the bactericide solution is marked as x, otherwise, the bactericide solution is marked as ○.
2. Test of Sterilization Effect
The test method comprises the following steps: the tube-disc method is adopted. Shaking the experimental strains evenly, using a liquid transfer gun to transfer 50ul of the bacterial suspension onto prepared agar plates, and spreading and coating evenly, wherein each of the 3 experimental strains is coated with 10 plates; sterilized oxford cups were then placed on the agar surface at equal intervals, 4 per dish, and after all had been lined up, were allowed to stand on a clean bench for 10 minutes. 200ul of the bactericide of the example was sucked up with a 1ml sterilizing pipette, and injected into the well-placed Oxford cups, 3 Oxford cups in each dish were injected with the water extract, and the other Oxford cup was injected with 200ul of sterilized ultrapure water as a control, and then carefully sealed with a sealing film. One bacterium was plated out to yield 3 plates of aqueous extract. Similarly, the same applies to the alcoholic extract (concentrated to 5ml with 100ml of 75% ethanol). And then culturing in a biochemical incubator at 37 ℃ for 24h, observing the growth condition of bacteria in the culture dish, measuring the diameter of an inhibition zone by using a micrometer, averaging the inhibition zone effects of the three medicaments in each culture dish, and accurately obtaining the data one bit behind the decimal point.
TABLE 1
Claims (10)
1. A preparation method of a plant bactericide is characterized by comprising the following steps:
(1) pulverizing the plant materials, and sieving to obtain mixture;
(2) adding 95% ethanol 5-10 times the weight of the mixture into the mixture, extracting at 60-70 deg.C for 1-3 hr, and filtering to obtain filtrate A;
(3) repeatedly extracting the rest residue with 1-3 times of 95% ethanol to obtain filtrate B, mixing filtrates A and B, and concentrating to 1/(10-20) of the total volume to obtain plant extract;
(4) adding the ginger and radix sophorae flavescentis extracting solution into the plant extract, and mixing and stirring uniformly to obtain the plant bactericide.
2. The method of preparing a plant bactericide as claimed in claim 1, wherein the plant material is selected from the group consisting of asarum, pulsatilla, andrographis, rhubarb, garlic, magnolia bark, eclipta, licorice, paris, forsythia, portulaca oleracea, chenopodium rubrum, schizonepeta, onion, agrimony, and scutellaria barbata.
3. The method of claim 1, wherein the plant material comprises Asarum sieboldii, Pulsatilla chinensis, Andrographis paniculata, Magnolia officinalis, forsythiae fructus, Portulaca oleracea, Chenopodium album, Agrimonia pilosa, and Scutellaria barbata.
4. The method for preparing a plant bactericide as claimed in claim 1, wherein the plant raw materials comprise, by weight, 1 to 5 parts of asarum, 1 to 5 parts of pulsatilla, 1 to 5 parts of andrographis paniculata, 1 to 5 parts of magnolia officinalis, 1 to 3 parts of forsythia, 1 to 3 parts of portulaca oleracea, 1 to 3 parts of chenopodium rubrum, 1 to 3 parts of agrimony and 1 to 3 parts of herba scutellariae barbatae.
5. The preparation method of the plant bactericide as claimed in claim 1, wherein the plant raw materials comprise, by weight, 2-5 parts of asarum, 2-5 parts of pulsatilla, 1-4 parts of andrographis paniculata, 1-4 parts of magnolia officinalis, 1-3 parts of forsythia, 1-3 parts of portulaca oleracea, 1-2 parts of chenopodium rubrum, 1-2 parts of agrimony and 1-2 parts of herba scutellariae barbatae.
6. The method for preparing plant bactericide as claimed in claim 5, wherein the volume ratio of the plant extract to the ginger and sophora flavescens extract in step (4) is 1: (1-1.2).
7. The method for preparing plant bactericide as claimed in claim 6, wherein the volume ratio of the plant extract to the ginger and sophora flavescens extract in step (4) is 1: 1.
8. a plant fungicide characterized by being produced by the production method according to any one of claims 1 to 7.
9. The plant fungicide according to claim 8, wherein said fungicide is in the form of oil, emulsifier, aqueous solvent, aerosol, spray, suspension, granule, powder, granule, capsule, drop, fumigant, aerosol, smoke suppressant.
10. The application of the plant bactericide is characterized in that the plant bactericide is used in the fields of foods, cosmetics, personal cleaning products, household cleaning products and daily chemical products.
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| CN112167272A (en) * | 2020-09-24 | 2021-01-05 | 朱明亮 | Pure plant Chinese herbal medicine bacteriostatic agent and preparation method and application thereof |
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