CN104560907B - 双羰基还原酶突变体及其应用 - Google Patents
双羰基还原酶突变体及其应用 Download PDFInfo
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- CN104560907B CN104560907B CN201410802549.2A CN201410802549A CN104560907B CN 104560907 B CN104560907 B CN 104560907B CN 201410802549 A CN201410802549 A CN 201410802549A CN 104560907 B CN104560907 B CN 104560907B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
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Abstract
Description
SEQ ID NO: | 位点 | 酶量a | 转化 | DE% | EE% |
1 | F231W+Q251H | 1wt | 80.14 | 90.08 | 100 |
2 | F231W+I94V+I236L | 3wt | 74.42 | 98.98 | 100 |
3 | I94V+F231W+I236L+Q251H | 1wt | 83.38 | 99 | 100 |
4 | F231W+I94V+Q251H | 1wt | 78.48 | 89.66 | 100 |
5 | I94V+V151Q+F231W+Q251H | 1wt | 77.68 | 89.8 | 100 |
6 | 母本(F231W+I94V) | 2wt | 72.91 | 89.89 | 100 |
SEQ ID NO: | 位点 | 酶量a | 转化 | DE% | EE% |
1 | F231W+Q251H | 3wt | 79.55 | 96.48 | 100 |
2 | F231W+I94V+I236L | 3wt | 70.43 | 99.16 | 100 |
3 | I94V+F231W+I236L+Q251H | 2wt | 81.9 | 99.12 | 100 |
4 | F231W+I94V+Q251H | 2wt | 75.95 | 93.54 | 100 |
5 | I94V+V151Q+F231W+Q251H | 2wt | 72.78 | 93.94 | 100 |
6 | 母本(F231W+I94V) | 8wt | 80.11 | 90.15 | 100 |
Claims (13)
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CN201410802549.2A CN104560907B (zh) | 2014-12-19 | 2014-12-19 | 双羰基还原酶突变体及其应用 |
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CN201410802549.2A CN104560907B (zh) | 2014-12-19 | 2014-12-19 | 双羰基还原酶突变体及其应用 |
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CN104560907A CN104560907A (zh) | 2015-04-29 |
CN104560907B true CN104560907B (zh) | 2017-12-22 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106834244B (zh) * | 2016-11-04 | 2021-07-06 | 凯莱英医药集团(天津)股份有限公司 | 脯氨酸羟化酶及其应用 |
CN107254454A (zh) * | 2017-05-16 | 2017-10-17 | 中国科学院成都生物研究所 | 一种羰基还原酶突变体及其应用 |
CN111394324B (zh) * | 2020-06-08 | 2020-09-08 | 凯莱英生命科学技术(天津)有限公司 | 酮还原酶突变体及其应用 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101429514A (zh) * | 2007-11-08 | 2009-05-13 | 陈依军 | 一种双羰基还原酶、其基因及其应用 |
CN102277338A (zh) * | 2011-08-02 | 2011-12-14 | 中国药科大学 | 双羰基还原酶突变体及其应用 |
CN103937760A (zh) * | 2014-05-06 | 2014-07-23 | 凯莱英医药集团(天津)股份有限公司 | 双羰基还原酶、其编码基因及应用 |
CN103937759A (zh) * | 2014-05-06 | 2014-07-23 | 凯莱英医药集团(天津)股份有限公司 | 双羰基还原酶、其编码基因及应用 |
CN103937761A (zh) * | 2014-05-06 | 2014-07-23 | 凯莱英医药集团(天津)股份有限公司 | 双羰基还原酶、其编码基因及应用 |
CN103966176A (zh) * | 2014-05-09 | 2014-08-06 | 凯莱英医药集团(天津)股份有限公司 | 双羰基还原酶突变体及其应用 |
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2014
- 2014-12-19 CN CN201410802549.2A patent/CN104560907B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101429514A (zh) * | 2007-11-08 | 2009-05-13 | 陈依军 | 一种双羰基还原酶、其基因及其应用 |
CN102277338A (zh) * | 2011-08-02 | 2011-12-14 | 中国药科大学 | 双羰基还原酶突变体及其应用 |
CN103937760A (zh) * | 2014-05-06 | 2014-07-23 | 凯莱英医药集团(天津)股份有限公司 | 双羰基还原酶、其编码基因及应用 |
CN103937759A (zh) * | 2014-05-06 | 2014-07-23 | 凯莱英医药集团(天津)股份有限公司 | 双羰基还原酶、其编码基因及应用 |
CN103937761A (zh) * | 2014-05-06 | 2014-07-23 | 凯莱英医药集团(天津)股份有限公司 | 双羰基还原酶、其编码基因及应用 |
CN103966176A (zh) * | 2014-05-09 | 2014-08-06 | 凯莱英医药集团(天津)股份有限公司 | 双羰基还原酶突变体及其应用 |
Non-Patent Citations (1)
Title |
---|
Genbank accession number:EEN87396.1;Sebastian, Y, et al.;《Genbank》;20130605;1 * |
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Effective date of registration: 20200414 Address after: 300457 No. 6, Dongting street, Tianjin economic and Technological Development Zone, Binhai New Area Co-patentee after: ASYMCHEM LIFE SCIENCE (TIANJIN) Co.,Ltd. Patentee after: ASYMCHEM LABORATORIES (TIANJIN) Co.,Ltd. Co-patentee after: ASYCHEM PHARMACEUTICALS (TIANJIN) Co.,Ltd. Co-patentee after: ASYMCHEM LABORATORIES (FUXIN) Inc. Co-patentee after: ASYMCHEM LABORATORIES (JILIN) Co.,Ltd. Co-patentee after: Shanghai kailaiying Biotechnology Co., Ltd Address before: 300457, No. 6, Dongting street, Tianjin economic and Technological Development Zone, Binhai New Area, Tianjin Co-patentee before: ASYMCHEM LIFE SCIENCE (TIANJIN) Co.,Ltd. Patentee before: ASYMCHEM LABORATORIES (TIANJIN) Co.,Ltd. Co-patentee before: ASYCHEM PHARMACEUTICALS (TIANJIN) Co.,Ltd. Co-patentee before: ASYMCHEM LABORATORIES (FUXIN) Inc. Co-patentee before: ASYMCHEM LABORATORIES (JILIN) Co.,Ltd. |
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