CN104498501B - 一种人源杀虫基因及其编码杀虫肽与应用 - Google Patents

一种人源杀虫基因及其编码杀虫肽与应用 Download PDF

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Publication number
CN104498501B
CN104498501B CN201410735997.5A CN201410735997A CN104498501B CN 104498501 B CN104498501 B CN 104498501B CN 201410735997 A CN201410735997 A CN 201410735997A CN 104498501 B CN104498501 B CN 104498501B
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insecticidal
gene
peptide
add
human
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Chinese (zh)
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CN104498501A (zh
Inventor
刘贤金
刘媛
谢雅晶
武爱华
张霄
徐重新
赵岩岩
仲建锋
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Jiangsu Academy of Agricultural Sciences
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Jiangsu Academy of Agricultural Sciences
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Priority to CN201410735997.5A priority Critical patent/CN104498501B/zh
Priority to BR112016014689-1A priority patent/BR112016014689B1/pt
Priority to EP15851632.8A priority patent/EP3067423B1/en
Priority to JP2016535244A priority patent/JP6211702B2/ja
Priority to PCT/CN2015/070423 priority patent/WO2016086513A1/zh
Priority to US15/103,515 priority patent/US9725521B2/en
Publication of CN104498501A publication Critical patent/CN104498501A/zh
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1278—Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4208—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig
    • C07K16/4233—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an idiotypic determinant on Ig against anti-bacterial Ig
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pest Control & Pesticides (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201410735997.5A 2014-12-05 2014-12-05 一种人源杀虫基因及其编码杀虫肽与应用 Active CN104498501B (zh)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN201410735997.5A CN104498501B (zh) 2014-12-05 2014-12-05 一种人源杀虫基因及其编码杀虫肽与应用
BR112016014689-1A BR112016014689B1 (pt) 2014-12-05 2015-01-09 Gene inseticida derivado de ser humano e peptídeo inseticida codificado pelo mesmo e aplicação dos mesmos
EP15851632.8A EP3067423B1 (en) 2014-12-05 2015-01-09 Human insecticidal gene and insecticidal peptide encoded thereby, and use thereof
JP2016535244A JP6211702B2 (ja) 2014-12-05 2015-01-09 ヒト殺虫遺伝子およびそれによりコードされた殺虫ペプチドならびに用途
PCT/CN2015/070423 WO2016086513A1 (zh) 2014-12-05 2015-01-09 一种人源杀虫基因及其编码杀虫肽与应用
US15/103,515 US9725521B2 (en) 2014-12-05 2015-01-09 Human-derived insecticidal gene and insecticidal peptide encoded thereby and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410735997.5A CN104498501B (zh) 2014-12-05 2014-12-05 一种人源杀虫基因及其编码杀虫肽与应用

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CN104498501A CN104498501A (zh) 2015-04-08
CN104498501B true CN104498501B (zh) 2017-07-18

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US (1) US9725521B2 (enExample)
EP (1) EP3067423B1 (enExample)
JP (1) JP6211702B2 (enExample)
CN (1) CN104498501B (enExample)
BR (1) BR112016014689B1 (enExample)
WO (1) WO2016086513A1 (enExample)

Families Citing this family (6)

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Publication number Priority date Publication date Assignee Title
CN105131123B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的牛源化改型抗体及其制备方法与应用
CN105085683B (zh) * 2015-09-16 2018-11-27 江苏省农业科学院 一种具有杀虫活性的猪源化改型抗体及其制备方法与应用
WO2017100632A1 (en) * 2015-12-11 2017-06-15 Arizona Board Of Regents On Behalf Of Arizona State University Human alzheimer's disease and traumatic brain injury associated tau variants as biomarkers and methods of use thereof
CN111732661A (zh) * 2020-06-11 2020-10-02 军事科学院军事医学研究院军事兽医研究所 抗h5n1病毒入胞抗体ptd-7b及其应用
CN113150161B (zh) * 2021-05-26 2022-06-17 江苏省农业科学院 苏云金芽孢杆菌Cry2Aa毒素的抗独特型单克隆抗体及应用
CN113512115B (zh) * 2021-08-09 2023-09-15 江苏省农业科学院 一种广谱识别小菜蛾中肠受体的抗Cry2Aa独特型单域抗体及其应用

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WO2007023782A1 (ja) * 2005-08-24 2007-03-01 Genecare Research Institute Co., Ltd. キラートキシン様タンパク質に対する中和抗体、およびその利用
JP2012529269A (ja) * 2009-06-05 2012-11-22 ジ オハイオ ステイト ユニヴァーシティ リサーチ ファウンデーション 生体材料、組成物及び方法
CN103571853B (zh) 2012-08-08 2016-01-20 中国科学院亚热带农业生态研究所 密码子优化的Cry2Aa基因与重组载体以及改变作物抗性的方法
CN103757049B (zh) 2013-12-24 2016-06-08 北京大北农科技集团股份有限公司 控制害虫的构建体及其方法
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Also Published As

Publication number Publication date
BR112016014689B1 (pt) 2022-06-07
US20170088633A1 (en) 2017-03-30
US9725521B2 (en) 2017-08-08
CN104498501A (zh) 2015-04-08
EP3067423B1 (en) 2020-07-15
EP3067423A4 (en) 2017-04-26
WO2016086513A1 (zh) 2016-06-09
JP6211702B2 (ja) 2017-10-11
EP3067423A1 (en) 2016-09-14
BR112016014689A2 (pt) 2017-12-12
JP2017502655A (ja) 2017-01-26

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