CN104491054A - 一种山楂叶提取物的生产工艺 - Google Patents
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Abstract
本发明公开了一种山楂叶提取物的生产工艺，属于植物提取技术领域。本发明通过原料预处理、酶解、微波提取和树脂纯化几个步骤提取并纯化山楂叶提取物，最后干燥得到终产品。本发明具有提取方便快捷、有效成分收率高、质量稳定、纯度高的特点，实用性强，适合工业化生产。
Description
技术领域
本发明涉及植物提取技术领域，具体涉及一种山楂叶提取物的生产工艺。
背景技术
山楂叶提取物为蔷薇科植物山里红或山楂的干燥叶经加工制成的提取物，含有多种有效成分，主要有黄酮和三萜两大类。山楂叶提取物有活血化瘀、宣通血脉、理气舒络的药理作用，对治疗冠心病、心绞痛、心肌梗塞、脑栓塞等心脑血管疾病和抑郁症等疾病有显著的疗效，同时，山楂叶提取物也能应用于保健品、食品等领域，具有较高的应用价值。而传统的提取方法收率较低，含量不高，且能源和原材料消耗量大，劳动强度高，生产成本高。
发明内容
本发明的目的是提供一种简单高效、高收率的山楂叶提取物生产工艺。
本发明是通过以下技术方案实现的：
一种山楂叶提取物的生产工艺，包括以下步骤：
（1）原料预处理：将山楂叶洗净，除去杂物和泥沙后粉碎至20-30目；
（2）酶解：按料液比1：10-1：12在处理后的原料中加入水，再加入纤维素酶于50-60℃、条件下保温沉淀4-6h，过滤后分别取滤液和滤渣待用；
（3）微波提取：将（2）中得到的滤渣用乙醇洗涤后按料液比1：8-1：10加入60-70%的乙醇，于60-80℃条件下微波提取15-30min，过滤后弃去滤渣，取滤液备用；
（4）树脂纯化：将（2）和（3）中得到的滤液合并后除去乙醇，再用水稀释至原体积的1.5-2倍，8000-10000r/min离心20-30min后取上清液用D101大孔吸附树脂吸附后用70-80%的乙醇洗脱，收集洗脱液，去除乙醇后干燥，即得。
其中，步骤（2）中酶的用量为底物的0.5-1%，步骤（3）中的微波处理功率为400-600W。
与现有技术相比，本发明的优势在于：
结合酶法与微波辅助提取，具有提取方便快捷、产品收率高、质量稳定、纯度高的特点，实用性强，适合工业化生产。
具体实施方式
实施例1
本发明的山楂叶提取物的生产工艺，包括以下步骤：
（1）原料预处理：将山楂叶洗净，除去杂物和泥沙后粉碎至20目；
（2）酶解：按料液比1：10在处理后的原料中加入水，再加入纤维素酶于60℃、条件下保温沉淀6h，过滤后分别取滤液和滤渣待用；
（3）微波提取：将（2）中得到的滤渣用乙醇洗涤后按料液比1：8加入70%的乙醇，于70℃条件下微波提取20min，过滤后弃去滤渣，取滤液备用；
（4）树脂纯化：将（2）和（3）中得到的滤液合并后除去乙醇，再用水稀释至原体积的2倍，10000r/min离心30min后取上清液用D101大孔吸附树脂吸附后用75%的乙醇洗脱，收集洗脱液，去除乙醇后干燥，即得。
其中，步骤（2）中酶的用量为底物的0.5%，步骤（3）中的微波处理功率为600W。
经检验，产品收率大于4.5%，其中总黄酮含量大于85%，牡荆素鼠李糖苷大于8.8%，金丝桃苷大于0.4%。
Claims (3)
1.一种山楂叶提取物的生产工艺，其特征在于，包括以下步骤：
（1）原料预处理：将山楂叶洗净，除去杂物和泥沙后粉碎至20-30目；
（2）酶解：按料液比1：10-1：12在处理后的原料中加入水，再加入纤维素酶于50-60℃、条件下保温沉淀4-6h，过滤后分别取滤液和滤渣待用；
（3）微波提取：将（2）中得到的滤渣用乙醇洗涤后按料液比1：8-1：10加入60-70%的乙醇，于60-80℃条件下微波提取15-30min，过滤后弃去滤渣，取滤液备用；
（4）树脂纯化：将（2）和（3）中得到的滤液合并后除去乙醇，再用水稀释至原体积的1.5-2倍，8000-10000r/min离心20-30min后取上清液用D101大孔吸附树脂吸附后用70-80%的乙醇洗脱，收集洗脱液，去除乙醇后干燥，即得。
2.根据权利要求1所述的一种山楂叶提取物的生产工艺，其特征在于，所述步骤（2）中酶的用量为底物的0.5-1%。
3.根据权利要求1所述的一种山楂叶提取物的生产工艺，其特征在于，所述步骤（3）中的微波处理功率为400-600W。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107156834A (zh) * | 2016-03-08 | 2017-09-15 | 云南奥咖生物技术有限公司 | 一种玛咖提取物的生产工艺 |
| CN109925351A (zh) * | 2019-01-22 | 2019-06-25 | 杭州师范大学 | 一种山楂叶中多酚类物质的提取方法 |
-
2014
- 2014-12-03 CN CN201410721602.6A patent/CN104491054A/zh active Pending
Non-Patent Citations (3)
| Title |
|---|
| 吴华: "大孔吸附树脂纯化山楂叶总黄酮", 《农机化研究》 * |
| 王晓等: "酶法提取山楂叶中总黄酮的研究", 《食品工业科技》 * |
| 王立娟: "微波辅助提取山楂叶总黄酮的条件研究", 《林产化学与工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107156834A (zh) * | 2016-03-08 | 2017-09-15 | 云南奥咖生物技术有限公司 | 一种玛咖提取物的生产工艺 |
| CN109925351A (zh) * | 2019-01-22 | 2019-06-25 | 杭州师范大学 | 一种山楂叶中多酚类物质的提取方法 |
| CN109925351B (zh) * | 2019-01-22 | 2021-07-20 | 杭州师范大学 | 一种山楂叶中多酚类物质的提取方法 |
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