CN104490773A

CN104490773A - Water-based emulsion containing lecithin and DHA and preparation method and application of water-based emulsion

Info

Publication number: CN104490773A
Application number: CN201410754652.4A
Authority: CN
Inventors: 张耿元; 王保红; 白东跃; 唐湘成
Original assignee: FUZHOU QIANZHENG PHARMACEUTICAL Co Ltd
Current assignee: FUZHOU QIANZHENG PHARMACEUTICAL Co Ltd
Priority date: 2014-12-11
Filing date: 2014-12-11
Publication date: 2015-04-08

Abstract

The invention provides an emulsion composition containing lecithin, DHA, other fat-soluble substances and purified water. The content of the purified water in the emulsion composition is more than 70% by weight, the weight ratio of the lecithin to the DHA is 1: (0.005-1), the particle size of fat balls in the emulsion is less than 5 mu m, and the other fat-soluble substances comprise one or more of the following components: arachidonic acid, linolenic acid, alpha-linolenic acid, gamma-linolenic acid, linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, phosphatidylserine, polyene phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl glycerol, phytosterol, plant sterol ester, plant stanol ester and medium and long-chain fatty acid. The invention further provides a preparation method of the emulsion composition and application of the emulsion composition in the functional aspects of supplementing phospholipids and unsaturated fatty acids by oral administration, protecting heart and brain blood vessels, reducing blood lipids, reducing blood pressure, treating fatty liver and the like.

Description

Water-based emulsion liquid containing lecithin and DHA and its preparation method and application

Technical field

The present invention relates to a kind of healthy product compositions, the aqua type fuel-water emulsion specifically containing lecithin and DHA, and preparation method thereof with its application.

Healthy product compositions of the present invention, refers to medicine, medical food, health food, nutraceutical or cosmetics etc.

Background technology

The common name phosphatidylcholine of lecithin, be described as " the 3rd nutrient " arranged side by side with protein, vitamin, since last century the nineties, phospholipid research has achieved significant effect in life sciences and brain science field, lecithin has good effect to the nutrition of human body with healthy, show: 1, human nutrition needs: the exogenous choline 90% of needed by human body is provided by lecithin, lecithin provides choline to have two large benefits: one, can be degraded into methylamine because of microbial action in intestinal unlike free choline; Secondly, it is the acquisition choline that methylates continuously by cephalin (PE) in liver and other fibrous tissue, and this building-up process needs certain hour, therefore when meals choline is not enough, the endogenous resource remaining lecithin (PC) in body can supplement human body needs; 2, to the regulating action of serum lipids: regulate serum lipid level to mean and can reduce cholesterol levels, the liver protecting, also can Improving memory power, booster immunization power and lipotropic vigor; 3, healthy heart: in the sixties in 20th century, scientists just finds that lecithin may have the effect of cardioprotection, in further studying, finally confirm that lecithin has positive role to health of heart, this is because it can the content of cholesterol regulating in human body, effectively reduce the sickness rate of cholesterol, hyperlipidemia and coronary heart disease; 4, useful brain: in cranial nerve cell, the content of lecithin accounts for 17% ~ 20% of its quality, " choline " is the basis of lecithin, the ample supply of lecithin ensures that " choline " synthesizes " acetylcholine " with " acetyl " in human body fully, " acetylcholine " is IC a kind of Information Conduction material, thus improve the activation degree of brain cell, improve memory and level of intelligence; 5, prevention and therapy arteriosclerosis: the effect that lecithin has emulsifying, cuts grease, blood circulation can be promoted, improve serum lipids, remove peroxide, Blood Cholesterol and neutral fat content are reduced, reduce fat in the holdup time of blood vessel, promote the dissipation of atherosis speckle, prevent the blood vessel internal membrane damage caused by cholesterol.Take lecithin, to hyperlipidemia and hypercholesterolemia, there is significant effect, thus can prevention and therapy arteriosclerosis (hypertension, myocardial infarction, cerebral hemorrhage); 6, Palmitoleic Acid: lecithin is the indispensable material of each cell of human body, if lacked, the regeneration capacity of Skin Cell will be reduced, cause pachylosis, have wrinkle, if suitably absorb lecithin, skin regeneration vigor just can ensure, adds the good hydrophilic of lecithin and lipophile, and skin is certainly just glossy.In addition, the inositol contained by lecithin or the main nutrient of hair, can suppress alopecia, makes poliosis slowly blackening, 7, slow down aging: people with age, memory can go down, its reason and acetyl choline content deficiency have certain relation, it is the main cause causing senile dementia that the neurotransmitter (acetylcholine) of brain reduces, compound required when acetylcholine is the transmission of nervous system information, and " choline " is the basis of lecithin, the ample supply of lecithin has the acetyl in enough choline and human body to be combined into " acetylcholine " by guarantee body, thus become brain sufficient Information Conduction material is provided, large brain-capacity directly absorbs lecithin and gallbladder alkali from blood, and be converted into acetylcholine very soon, long-term supplementary lecithin can slow down the process of hypomnesis, the generation of prevention or postponement senile dementia, 8, adjustment psychology: social competition is growing more intense, under people are in nervous environment and all pressure for a long time, often suffer from the diseases such as anxiety, irritability, irritability, insomnia, tinnitus, i.e. autonomic nervous dysfunction, is commonly called neurasthenia, often supplement lecithin, cerebral nerve can be made to obtain nutritional supplementation in time, and the duty of keeping fit, is beneficial to allaying tiredness, intensify brain cell, improve the disease such as irritability, irritability, insomnia caused because of nervous, 9, pulmonary function is activated: alveolar is the cell of picked-up oxygen in body, alveolar is moistening, the oxygen sucked easily dissolves, otherwise then causes body anoxia, and lecithin has good hydrophilic, alveolar can be made to keep moistening, thus improve the intake of body oxygen, more weak or smoker's alveolar dry, take the photograph hypoxgia for pulmonary function, and motion, easier to be tired because of anoxia after work, should lecithin be supplemented more.

DHA, docosahexenoic acid, be commonly called as NAOHUANGJIN, be a kind of to the very important unsaturated fatty acid of human body, belong to the important member in omega-3 unsaturated fatty acid family, a kind of main component that DHA is nervous system cell growth and maintains, be brain and amphiblestroid important composition composition, in human brain cortex, content is up to 20%, and in eye retina, proportion is maximum, account for 50%, to intelligence and visual acuity and health most important.DHA has good effect to the nutrition of human body with healthy, show: 1, promote fetal brain development: in the pregnancy period, DHA can optimize the constituent of the phospholipid of fetal brain pyramidal cell, especially fetus is full after 5 months, the audition of fetus, vision, sense of touch are stimulated, the sensorial neuron of fetal brain cortex can be caused to increase more dendron, and this just needs parent to supply the more DHA of fetus simultaneously; 2, the maturation of retina light sensitive cells is promoted: DHA not only has material impact to fetal brain development, and plays an important role to the maturation of retina light sensitive cells.Anemia of pregnant woman by taking in DHA in the pregnancy period, is then transported to fetal brain and retina, neurocyte Maturity is improved; 3, promote brain cell to grow: in cerebral cortex, DHA is not only the Main Ingredients and Appearance of nerve conduction cell, also be the Main Ingredients and Appearance that cell membrane is formed, most DHA can not digest by gastric juice, and directly enter blood, by the absorbed organ such as liver or brain, because DHA can enter brain cell via blood brain barrier, and be both and belong to the EPA of unsaturated fatty acid and hypo-linolenic acid cannot be absorbed by brain by blood brain barrier; 4, postpartum depression is reduced: research display, the women of China 50 ~ 75% is along with one section of postpartum depression is experienced in the birth of child, the new mother of 10 ~ 15% can become very strong, be called " postpartum depression " by buzzword, postpartum depression not only can serious threat puerpera healthy, and the growth of baby can be affected, cause baby that affective disorder, dystropy occur.Enough DHA can reduce the generation of postpartum depression; 5, inflammation-inhibiting: DHA can suppress the formation of inflammation precursor, so have antiinflammation; 6, reduce blood fat, prevent cardiovascular disease: DHA can reduce the formation of triglyceride in blood, cholesterol and pre-preventing thrombosis; 7, protect brain and protect brain, improve old man's dementia: due to advancing age, DHA in brain will reduce gradually, easily cause the degeneration of brain function, in fact, brain cell can constantly be grown up before two to three years old, after growing to manhood, then can reduce gradually, according to investigation, 20 to three ten years old time, brain cell can reduce gradually with the ratio of 100,000, and even so, DHA still has the strength of the brain cell activation making to be left, improve adult fully, especially person in middle and old age's man memory and learning capacity; 8, influenza virus is suppressed to be copied: to find Related Component in the docosahexenoic acid (DHA) that fish oil is rich in by mouse experiment research, can effectively suppress influenza virus to breed; 9, beauty treatment and defying age: by triggering the cell mitochondrial energy of cyclophorase effect restore peace weighing apparatus by burn-in effects; reduce chill mark; lifting degree of compacting and the colour of skin; in addition; promote the synthesis of ATP prevent internal aging while protection skin avoid free radical, chemical substance and pollution to cause external weathering; can skin connective tissue be rebuild, keep elasticity and the compactness of skin, also protect and optimize the metabolism of Skin Cell.

Lecithin or DHA have been processed to various health food, as soft capsule, tablet etc., also do not find the compound of lecithin and DHA.

According to the concept of pharmaceutics, bioavailability (Bioavailability) refers to that medicine is rapidly absorbed into sanguimotor producing level and utilizes speed.

Adverse influence is, the oral absorption availability of DHA is also bad, and the existence of DHA largely affects its digesting and assimilating in human body, no matter ethyl ester type and methyl ester type or triglyceride type, all absorbed in the mode of Passive diffusion in vivo, ethyl ester type DHA absorbance is only about 20%, and the triglyceride type DHA absorbance that natural form exists, higher than ethyl ester type, also only has about 45%; In addition, different formula combination, dosage form, preparation method or existing way, its bioavailability is also significantly different.

Summary of the invention

Inventor surprisingly finds, the compositions of lecithin and DHA, be conducive to fully absorbing of lecithin and DHA, the producing level of large increase DHA, especially lecithin and DHA are made the microemulsion formulations of water base property, for improving the biological utilisation degree of lecithin and DHA and utilizing speed all significant.

The invention provides a kind of emulsion compositions containing lecithin, DHA and water, in described compositions, the content of water is greater than 70% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm;

Preferably, the invention provides a kind of emulsion compositions containing lecithin, DHA and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 3 μm, such as, make the Oil globule particle diameter in liquid be 2 μm, 1 μm etc.;

Preferably, the invention provides a kind of emulsion compositions containing lecithin, DHA and water, in described compositions, the content of water is greater than 80% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 2 μm, such as, make the Oil globule particle diameter in liquid be 1 μm, 0.5 μm, 0.3 μm etc.

Another aspect of the present invention provides lecithin and DHA with the combination of arbitrary proportion; preferably with the present invention's combination that the ratio of synergistic dosage exists; or the present invention's combination that the ratio of the dosage of the dosage of the stability of DHA in emulsion and its bioavailability of raising exists preferably can be protected with lecithin; such as lecithin and DHA take weight ratio as 1:(0.005 ~ 1) ratio combination

Preferably lecithin and DHA take weight ratio as 1:(0.006 ~ 0.8) ratio combination,

Preferably lecithin and DHA take weight ratio as 1:(0.008 ~ 0.6) ratio combination,

Preferably lecithin and DHA take weight ratio as 1:(0.01 ~ 0.5) ratio combination,

Preferably lecithin and DHA take weight ratio as 1:(0.02 ~ 0.3) ratio combination,

Preferably lecithin and DHA take weight ratio as 1:(0.03 ~ 0.2) ratio combination,

Preferably lecithin and DHA take weight ratio as 1:(0.05 ~ 0.1) ratio combination,

Preferably lecithin and DHA take weight ratio as 1:(0.06 ~ 0.1) ratio combination,

Concrete, such as preferably lecithin and DHA take weight ratio as the ratio combination of 1:0.01,1:0.02,1:0.04,1:0.05,1:0.06,1:0.08,1:0.09,1:0.1,1:0.2,1:0.3,1:0.4,1:0.5,1:0.6,1:0.7,1:0.8,1:0.9,1:1 etc.

Further, the invention provides a kind of emulsion compositions containing lecithin, DHA, water and one or more compositions following: arachidonic acid, linolenic acid (comprising alpha-linolenic acid or gamma-Linolenic acid), linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, Phosphatidylserine, polyene phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, phosphatidylinositols, phosphatidyl glycerol, plant sterol, plant sterol ester, phytostanol ester, middle long-chain fatty acid.

Should illustrate and it will be understood by those skilled in the art that, lecithin of the present invention, DHA, arachidonic acid, linolenic acid (comprising alpha-linolenic acid or gamma-Linolenic acid), linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, Phosphatidylserine, polyene phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, phosphatidylinositols, phosphatidyl glycerol, plant sterol, plant sterol ester, phytostanol ester, middle long-chain fatty acid etc. are all fats or liposoluble substance, normally extract from vegetable oil or egg yolk, or further resynthesis compound after extracting from vegetable oil, such as, lecithin extracts from soybean oil or egg yolk, Phosphatidylserine is for raw material with lecithin and Serine, after adopting phospholipase conversion reaction, obtained after purification, again such as, plant sterol comprises the cupreol, campesterol, stigmasterol etc. that extract from soybean oil, Oleum Brassicae campestris, Semen Maydis oil, Oleum Helianthi, Tall oil,

On the other hand, lecithin of the present invention, DHA, arachidonic acid, linolenic acid, alpha-linolenic acid, gamma-Linolenic acid, linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, Phosphatidylserine, polyene phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, phosphatidylinositols, phosphatidyl glycerol, plant sterol, plant sterol ester, phytostanol ester, these fats such as middle long-chain fatty acid or liposoluble substance, usual meeting is present in Vegetable oil lipoprotein with the ratio of certain content, special, above-mentioned liposoluble substance of the present invention also comprises the vegetable oil containing these liposoluble substances, such as, lecithin is present in soybean oil extract or oleic acid with the content of 40 ~ 60%, lecithin, PHOSPHATIDYL ETHANOLAMINE, the ratio that phosphatidylinositols and phosphatidyl glycerol are greater than 35% with total phospholipids content is present in krill oil, DHA is present in the algae oil splitting kettle algae (or I Ken Shi kettle algae or Kou Shi Crypthecodinium cohnii) with the content being greater than 35%, linolenic acid is present in peony seed oil or eucommia ulmoides seed oil with the content being greater than 40%, and linoleic acid is present in silybum marianum seed oil with the content of 20% ~ 40%, safflower oil, Suaeda salsa seed oil or tea-seed oil in, etc.,

Should illustrate and understand, lecithin of the present invention, DHA, arachidonic acid, linolenic acid, alpha-linolenic acid, gamma-Linolenic acid, linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, Phosphatidylserine, polyene phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, phosphatidylinositols, phosphatidyl glycerol, plant sterol, plant sterol ester, phytostanol ester or middle long-chain fatty acid etc. both can be that sterling is applied to the present invention, also can be containing lecithin, DHA, arachidonic acid, linolenic acid, alpha-linolenic acid, gamma-Linolenic acid, linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, Phosphatidylserine, polyene phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, phosphatidylinositols, phosphatidyl glycerol, plant sterol, plant sterol ester, the non-sterling of phytostanol ester or middle long-chain fatty acid is applied to the present invention, such as be applied to the present invention with Vegetable oil or plant extract oil, such as, the donor of algae oil as DHA splitting kettle algae (or I Ken Shi kettle algae or Kou Shi Crypthecodinium cohnii) that DHA content is greater than 35%, directly apply to the present invention, linolenic acid content be greater than 40% in peony seed oil or eucommia ulmoides seed oil as linolenic donor, directly apply to the present invention, linoleic acid content 20% ~ 55% silybum marianum seed oil, safflower oil, Suaeda salsa seed oil or tea-seed oil, as linoleic donor, directly apply to the present invention.

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, arachidonic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, alpha-linolenic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, gamma-Linolenic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, linoleic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, conjugated linoleic acid (or conjugated linoleic acid glyceride) and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, Phosphatidylserine and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, polyene phosphatidylcholine and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, PHOSPHATIDYL ETHANOLAMINE and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, phosphatidyl-4 alcohol and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, phosphatidyl glycerol and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of containing lecithin, DHA, plant sterol (or plant sterol ester, or phytostanol ester) and the emulsion compositions of water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, linoleic acid, middle long-chain fatty acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, conjugated linoleic acid, gamma-Linolenic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of containing lecithin, DHA, arachidonic acid, conjugated linoleic acid, alpha-linolenic acid, plant sterol (or plant sterol ester, or phytostanol ester) and the emulsion compositions of water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, arachidonic acid, alpha-linolenic acid, middle long-chain fatty acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, arachidonic acid, conjugated linoleic acid, alpha-linolenic acid, middle long-chain fatty acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of containing lecithin, DHA, arachidonic acid, conjugated linoleic acid, Phosphatidylserine, plant sterol (or plant sterol ester, or phytostanol ester) and the emulsion compositions of water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, arachidonic acid, conjugated linoleic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, polyene phosphatidylcholine, conjugated linoleic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, arachidonic acid, polyene phosphatidylcholine, linoleic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, arachidonic acid, PHOSPHATIDYL ETHANOLAMINE, conjugated linoleic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, arachidonic acid, phosphatidylinositols, conjugated linoleic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, arachidonic acid, phosphatidyl glycerol, conjugated linoleic acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, arachidonic acid, conjugated linoleic acid, middle long-chain fatty acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of emulsion compositions containing lecithin, DHA, gamma-Linolenic acid, middle long-chain fatty acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of containing lecithin, DHA, arachidonic acid, conjugated linoleic acid, plant sterol (or plant sterol ester, or phytostanol ester), the emulsion compositions of middle long-chain fatty acid and water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

Concrete, the invention provides a kind of containing lecithin, DHA, arachidonic acid, gamma-Linolenic acid, conjugated linoleic acid, Phosphatidylserine, plant sterol (or plant sterol ester, or phytostanol ester) and the emulsion compositions of water, in described compositions, the content of water is greater than 75% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm, such as, the Oil globule particle diameter in liquid is made to be 3 μm, 1 μm, 0.5 μm, 0.3 μm etc.

Further, the emulsion compositions containing lecithin, DHA and water that the present invention is above-mentioned, optionally can also contain sweeting agent, described sweeting agent is selected from least one in steviol glycosides, fructose, xylitol, sucrose, sucralose, Sorbitol, high fructose syrup, protein sugar, aspartame, acesulfame potassium, stachyose, neotame, glucose, Mel, polyglucose.

Further; in the present composition; can also other one or more functional components be contained, nutrition or other compositions wholesome etc. such as such as inositol, L-carnitine, vitamin B2, vitamin B12, vitamin C, vitamin E, vitamin D, taurine, oligomeric isomaltose, probiotic bacteria, bacillus bifidus, Radix Ginseng, Antrodia Camphorata, Herba Epimedii, Cordyceps militaris (L.) Link., Lepidinm meyenii Walp, eucommia Bark male flower.

Emulsion compositions of the present invention, the emulsion that a kind of chyle fat is formed, be one complicated, thermodynamic stable system, especially different oils or fat-soluble ingredient, because the fat-water distribution of each component is with different in conjunction with energy state, stability for whole system is vital, multi-component fat breast system, be easy to occur fat floating, the wild effects such as flocculation or layering, production method and technique, decide form and the size of Oil globule in emulsion, and whether can there is the microphenomenoies such as gathering, thus affect the fat floating of emulsion in macroscopic aspect, the instability such as flocculation or layering, certainly, also emulsion digesting and assimilating and utilizing in human body is affected.

Further, the invention provides a kind of preparation method of the emulsion compositions containing lecithin, DHA and purified water, it comprises: lecithin, DHA mixed with water, emulsifying and make uniform liquid, wherein the content of water is greater than 70% with mass ratio range, and the Oil globule particle diameter in emulsion is less than 5 μm.

Especially, the invention provides a kind of preparation method of the emulsion compositions containing lecithin, DHA, other liposoluble substance and purified water, it comprises:

(1) prepare burden: take lecithin, DHA, other liposoluble substance by formula, add in purified water, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

(2) emulsifying: the liquid prepared in (1) is placed in emulsion tank, nitrogen or carbon dioxide is passed into continuously bottom emulsion tank, temperature of liquid be 45 ~ 65 DEG C, under speed of agitator is greater than the condition of 2000r/min, carry out emulsification procedure 5 ~ 35min, then after emulsion being cooled to 25 DEG C in 5min, stop stirring and stopping passing into nitrogen or carbon dioxide, leave standstill;

Preferably, nitrogen or carbon dioxide intake are 10 ~ 80L/min, and emulsifying speed of agitator is not less than 2800r/min;

(3) homogenizing: the emulsion that step (2) is obtained is filtered through 100 ~ 500 orders, filtrate is in temperature 50 ~ 98 DEG C, pressure is that 15 ~ 58MPa carries out homogenizing, optionally repeatedly homogenizing can be carried out, and make the Oil globule particle diameter in emulsion be less than 5 μm, the Oil globule particle diameter in liquid is preferably made to be less than 3 μm, the Oil globule particle diameter in liquid is such as made to be 2 μm, 1 μm, 0.5 μm, 0.3 μm etc., homogenizing, refer to that the feed liquid of material is in extruding, material refinement is made under the triple role that thump and decompression expand, broken is more tiny, thus make material energy evenly mutual mixing, whole product system is more stable, preferably, the present invention proposes homogenizing treatment technology, through repeatedly homogenizing process, make lecithin, DHA, other liposoluble substance, the Homogeneous phase mixing such as purified water also keep stable homogeneous,

(4) fill, sterilizing: the emulsion after homogenizing is carried out vacuum outgas, canned, sealing, and sterilizing 4 seconds ~ 35 minutes under 85 ~ 135 DEG C of conditions;

(5) pack, check, obtain product of the present invention;

In described emulsion compositions, the content of purified water is greater than 70% with mass ratio range, the weight ratio of lecithin and DHA is 1:(0.005 ~ 1), and the Oil globule particle diameter in emulsion is less than 5 μm, in preferred described emulsion compositions, the content of purified water is greater than 75% with mass ratio range, the weight ratio of lecithin and DHA is 1:(0.008 ~ 0.6), and the Oil globule particle diameter in emulsion is less than 3 μm;

Other liposoluble substance described comprises one or more compositions following: arachidonic acid, alpha-linolenic acid, gamma-Linolenic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, Phosphatidylserine, polyene phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, phosphatidylinositols, phosphatidyl glycerol, plant sterol, plant sterol ester, phytostanol ester, middle long-chain fatty acid.

The present invention surprisingly finds, the obtained emulsion containing lecithin, DHA, other liposoluble substance and water, Oil globule particle diameter distributed effect is good, so-called particle size distribution effect, refer to size homogeneity, difference in size rate, form regularity etc., it is the most critical factor of reflection Oil globule particle diameter and stability of emulsion.Especially in emulsification procedure, pass into gas (nitrogen or carbon dioxide), the blending and stirring and the cavitation that form gas and liquid when high-speed stirred is sheared are sheared, various fat-soluble ingredient energy rapid dispersion is made to be emulsified into the Oil globule of uniform particle diameter, little and the narrow diameter distribution of Oil globule particle diameter, and, adopt, in 5min, emulsion is quickly cooled to 25 DEG C, the form of Oil globule is made to present homogeneous, regular spherical shape, Oil globule drop is stoped to regroup, emulsion intercalation method is had and significantly improves, and significantly improve its bioavailability.

Further, the invention provides a kind of emulsion compositions containing lecithin, DHA, other liposoluble substance and purified water, in described emulsion compositions, the content of purified water is greater than 70% with mass ratio range, the weight ratio of lecithin and DHA is 1:(0.005 ~ 1), and the Oil globule particle diameter in emulsion is less than 5 μm, in preferred described emulsion compositions, the content of purified water is greater than 75% with mass ratio range, the weight ratio of lecithin and DHA is 1:(0.008 ~ 0.6), and the Oil globule particle diameter in emulsion is less than 3 μm; Other liposoluble substance described comprises one or more compositions following: arachidonic acid, alpha-linolenic acid, gamma-Linolenic acid, linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, Phosphatidylserine, polyene phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, phosphatidylinositols, phosphatidyl glycerol, plant sterol, plant sterol ester, phytostanol ester, middle long-chain fatty acid;

Wherein, the preparation method of described emulsion compositions comprises:

(3) homogenizing: the emulsion that step (2) is obtained is filtered through 100 ~ 500 orders, filtrate in temperature 50 ~ 98 DEG C, pressure be that 15 ~ 58MPa carries out homogenizing, optionally repeatedly homogenizing can be carried out, and make the Oil globule particle diameter in emulsion be less than 5 μm, preferably make the Oil globule particle diameter in liquid be less than 3 μm, such as, make the Oil globule particle diameter in liquid be 2 μm, 1 μm, 0.5 μm, 0.3 μm etc.;

(5) pack, check, to obtain final product;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, alpha-linolenic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, gamma-Linolenic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, linoleic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, conjugated linoleic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, conjugated linoleic acid glyceride and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, Phosphatidylserine and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, polyene phosphatidylcholine and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, PHOSPHATIDYL ETHANOLAMINE and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, phosphatidylinositols and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, phosphatidyl glycerol and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, plant sterol (or plant sterol ester, or phytostanol ester) and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, middle long-chain fatty acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, conjugated linoleic acid, gamma-Linolenic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, conjugated linoleic acid, alpha-linolenic acid, plant sterol (or plant sterol ester, or phytostanol ester) and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, alpha-linolenic acid, middle long-chain fatty acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, conjugated linoleic acid, alpha-linolenic acid, middle long-chain fatty acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, linoleic acid, Phosphatidylserine, plant sterol (or plant sterol ester, or phytostanol ester) and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, conjugated linoleic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, polyene phosphatidylcholine, conjugated linoleic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, polyene phosphatidylcholine, conjugated linoleic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, PHOSPHATIDYL ETHANOLAMINE, conjugated linoleic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, phosphatidylinositols, conjugated linoleic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, phosphatidyl glycerol, conjugated linoleic acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, conjugated linoleic acid, middle long-chain fatty acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, gamma-Linolenic acid, middle long-chain fatty acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, conjugated linoleic acid, plant sterol (or plant sterol ester, or phytostanol ester), middle long-chain fatty acid and purified water;

Concrete, described emulsion compositions contains lecithin, DHA, arachidonic acid, gamma-Linolenic acid, conjugated linoleic acid, Phosphatidylserine, plant sterol (or plant sterol ester, or phytostanol ester) and purified water;

Further, the emulsion compositions that the present invention is above-mentioned, optionally can also contain sweeting agent, described sweeting agent is selected from least one in steviol glycosides, fructose, xylitol, sucrose, sucralose, Sorbitol, high fructose syrup, protein sugar, aspartame, acesulfame potassium, stachyose, neotame, glucose, Mel, polyglucose.

Further; in the emulsion compositions that the present invention is above-mentioned; can also other one or more functional components be contained, nutrition or other compositions wholesome etc. such as such as inositol, L-carnitine, vitamin B2, vitamin B12, vitamin C, vitamin E, vitamin D, taurine, oligomeric isomaltose, probiotic bacteria, bacillus bifidus, Radix Ginseng, Antrodia Camphorata, Herba Epimedii, Cordyceps militaris (L.) Link., Lepidinm meyenii Walp, eucommia Bark male flower.

Should illustrate, the present invention contains the emulsion compositions of lecithin, DHA, other liposoluble substance and purified water, special, the filtration order number that it adopts is 100 ~ 500 orders, most preferably filtering order number is 250 ~ 500 orders, the heating temperature of preferred feed 35 ~ 80 DEG C, and preferred homogenization pressure is 25-58Mpa, the vacuum of preferred vacuum outgas is 0.04 ~ 0.09Mpa, preferred sterilising conditions be 115 ~ 121 DEG C 20 minutes.

Further, lecithin of the present invention, DHA and other liposoluble substance have good pharmacology synergism, for health, especially at oral supplementation phospholipid and unsaturated fatty acid, protection cardiovascular and cerebrovascular vessel, blood fat reducing, blood pressure lowering, the effect aspects such as treatment fatty liver, there is good synergism, present invention also offers above-mentioned containing containing lecithin, DHA, the emulsion compositions of other liposoluble substance and purified water is for the preparation of oral supplementation phospholipid and unsaturated fatty acid, protection cardiovascular and cerebrovascular vessel, blood fat reducing, blood pressure lowering, application in the healthy product for the treatment of fatty liver, described healthy product comprise medicine, medical food, health food or nutraceutical, in addition, the emulsion compositions that the present invention contains lecithin, DHA, other liposoluble substance and purified water can also be applied to cosmetics, for the nursing of skin and beauty treatment, etc.

The present invention surprisingly finds, obtained by the present invention containing lecithin, DHA, the emulsion of other liposoluble substance and water, Oil globule particle diameter distributed effect is good, especially in emulsification procedure, pass into gas (nitrogen or carbon dioxide), the blending and stirring and the cavitation that form gas and liquid when high-speed stirred is sheared are sheared, various fat-soluble ingredient energy rapid dispersion is made to be emulsified into the Oil globule of uniform particle diameter, little and the narrow diameter distribution of particle diameter, and, adopt, in 5min, emulsion is quickly cooled to 25 DEG C, the form of Oil globule is presented homogeneous, regular spherical shape, Oil globule drop can be well stoped to regroup, significantly improve emulsion intercalation method,

In addition, the present invention contains the emulsion of lecithin, DHA, other liposoluble substance and water, the little and narrow diameter distribution of Oil globule particle diameter, and close to nano-scale, the oral absorption for it utilizes, significant, and significantly improves its bioavailability;

Such as, be to be understood that, oral preparation needs to absorb through gastrointestinal just can enter blood of human body to reach physiological action, and the quality of oral absorption, directly affects the therapeutic effect of medicine.According to the concept of pharmaceutics, bioavailability (Bioavailability) refers to that preparation is rapidly absorbed into sanguimotor producing level and utilizes speed.Compare the preparation of other non-emulsified liquid type, the emulsion that the present invention contains lecithin, DHA, other liposoluble substance and water has excellent water-oiliness dissolubility, the oral drug preparation taking liposoluble substance as active ingredient is entered in sanguimotor degree and speed in absorption, there is significant facilitation and meaning.Preparation enters the absorption process after gastrointestinal, divide two stages, namely disintegrate or dispersion and two stages of Gastrointestinal Wall Absorption, first need to scatter in gastric juice or intestinal juice, and then contact and be attached to gastrointestinal wall and carry out absorbing and entering blood, this two stages all can affect the performance with drug effect that absorbs of medicine, liposoluble substance is prepared as water dispersible good, extra small and the emulsion of narrow diameter distribution of Oil globule particle diameter, stripping and the rate of dispersion of liposoluble substance are significantly accelerated, substantially increase degree and the rate of dispersion of its disintegrate dispersion in gastric juice or intestinal juice, then the contact of liposoluble substance and gastrointestinal wall is more abundant, be attached to Gastrointestinal Wall Absorption and to enter the active ingredient of blood also more abundant, this raising for the bioavailability of liposoluble substance is significant.

detailed description of the inventionin implementation process of the present invention, those of ordinary skill in the art are not departing from the scope of the present invention various embodiment that the basis with spirit produces and are modifying apparent and be easily carry out.By the following examples application of the present invention etc. done and illustrate further, but do not represent embodiment limitation of the present invention.

Embodiment 1, lecithin DHA emulsion formulations and preparation method thereof

Recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5.5%, is preferably 2.5%,

DHA 0.03% ~ 1.5%, is preferably 0.55%,

Sucralose 0 ~ 0.05%, is preferably 0.01%,

Purified water adds to 100%;

Preparation: (1) prepares burden: take sucralose by formula and add in purified water, make to dissolve completely, separately take lecithin, DHA, add in purified water successively, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

(2) emulsifying: the liquid prepared in (1) is placed in emulsion tank, nitrogen or the carbon dioxide of 20 ~ 30L/min is passed into continuously bottom emulsion tank, temperature of liquid be 45 ~ 65 DEG C, under speed of agitator is greater than the condition of 2500r/min, carry out emulsification procedure 10 ~ 20min, then interlayer cooling water is passed into, after making emulsion be cooled to 25 DEG C in 5min, stop stirring and stopping passing into nitrogen or carbon dioxide, leave standstill;

(3) homogenizing: emulsion obtained for step (2) is filtered through 200 ~ 500 orders, filtrate in temperature 50 ~ 98 DEG C, pressure be that 25 ~ 35MPa carries out homogenizing;

(4) fill, sterilizing: the emulsion after homogenizing is carried out vacuum outgas, canned, sealing, and 115 ~ 121 DEG C of sterilizings 20 minutes;

(5) pack, check, obtain lecithin DHA emulsion formulations;

Through laser diffraction analyzer, the Oil globule particle size distribution of this emulsion formulations is at 2 ~ 3 μm, and taste is slightly sweet, has the fragrance of oils and fats.

Embodiment 2, composite phospholipid DHA emulsion formulations and preparation method thereof

Recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%, is preferably 4.0%,

DHA 0.03% ~ 1%, is preferably 0.5%,

Conjugated linoleic acid 0.5% ~ 5%, is preferably 1.5%,

Arachidonic acid 0 ~ 3.5%, is preferably 0.5%,

Purified water adds to 100%;

Preparation: (1) prepares burden: take lecithin, DHA, conjugated linoleic acid, arachidonic acid by formula, add in purified water successively, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

(2) emulsifying: the liquid prepared in (1) is placed in emulsion tank, nitrogen or the carbon dioxide of 20 ~ 30L/min is passed into continuously bottom emulsion tank, temperature of liquid be 45 ~ 65 DEG C, under speed of agitator is greater than the condition of 2800r/min, carry out emulsification procedure 15 ~ 30min, then interlayer cooling water is passed into, after making emulsion be cooled to 25 DEG C in 5min, stop stirring and stopping passing into nitrogen or carbon dioxide, leave standstill;

(3) homogenizing: emulsion obtained for step (2) is filtered through 200 ~ 500 orders, filtrate in temperature 50 ~ 98 DEG C, pressure be that 45 ~ 55MPa carries out homogenizing;

(5) pack, check, obtain composite phospholipid DHA emulsion formulations;

Through laser diffraction analyzer, the Oil globule particle size distribution of this emulsion formulations is at 0.5 ~ 1 μm, and taste has the fragrance of oils and fats, optionally can add appropriate sweeting agent or taste modifying agent.

Embodiment 3, composite phospholipid DHA emulsion formulations and preparation method thereof

Recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 8%, is preferably 5.0%,

DHA 0.03% ~ 1.5%, is preferably 0.5%,

Peony seed oil linolenic acid (linolenic acid content >=38%) 0.5% ~ 10%, is preferably 1%(in linolenic acid content),

Purified water adds to 100%;

Preparation: (1) prepares burden: take lecithin, DHA, peony seed oil linolenic acid by formula, add successively in purified water, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

(5) pack, check, obtain composite phospholipid DHA emulsion formulations;

Embodiment 4, composite phospholipid DHA emulsion formulations and preparation method thereof

Recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%, is preferably 3.5%,

DHA 0.03% ~ 1%, is preferably 0.5%,

Conjugated linoleic acid 0.5% ~ 5%, is preferably 1.5%,

Phosphatidylserine 0.1% ~ 5%, is preferably 0.2%,

Purified water adds to 100%;

Preparation: (1) prepares burden: take lecithin, DHA, conjugated linoleic acid, Phosphatidylserine by formula, add in purified water successively, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

(5) pack, check, obtain composite phospholipid DHA emulsion formulations;

Embodiment 5, composite phospholipid DHA emulsion formulations and preparation method thereof

Recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%, is preferably 3.5%,

DHA 0.03% ~ 1%, is preferably 0.5%,

Conjugated linoleic acid 0.5% ~ 5%, is preferably 1.5%,

Plant sterol 0.2% ~ 1%, is preferably 0.5%,

Purified water adds to 100%;

Preparation: (1) prepares burden: take lecithin, DHA, conjugated linoleic acid, plant sterol by formula, add in purified water successively, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

(5) pack, check, obtain composite phospholipid DHA emulsion formulations;

Embodiment 6, composite phospholipid DHA emulsion formulations and preparation method thereof

Recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%, is preferably 3.5%,

DHA 0.03% ~ 1%, is preferably 0.5%,

Middle long-chain fatty acid 0.5% ~ 5%, is preferably 1.5%,

Purified water adds to 100%;

Preparation: (1) prepares burden: take lecithin, DHA, middle long-chain fatty acid by formula, add successively in purified water, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

(5) pack, check, obtain composite phospholipid DHA emulsion formulations;

Embodiment 7, composite phospholipid olefin(e) acid emulsion formulations and preparation method thereof

Recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%, is preferably 3%,

DHA 0.03% ~ 1%, is preferably 0.5%,

Conjugated linoleic acid 0.02% ~ 1%, is preferably 0.5%,

PHOSPHATIDYL ETHANOLAMINE 0.02% ~ 1%, is preferably 0.5%,

Arachidonic acid 0.02% ~ 1%, is preferably 0.5%,

Purified water adds to 100%;

Preparation: (1) prepares burden: take lecithin, DHA, conjugated linoleic acid, PHOSPHATIDYL ETHANOLAMINE, arachidonic acid by formula, add in purified water successively, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

(5) pack, check, obtain composite phospholipid olefin(e) acid emulsion formulations;

Embodiment 8, composite phospholipid olefin(e) acid emulsion formulations and preparation method thereof

Recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%, is preferably 2.5%,

DHA 0.03% ~ 1%, is preferably 0.5%,

Conjugated linoleic acid 0.02% ~ 1%, is preferably 0.3%,

Arachidonic acid 0.02% ~ 1%, is preferably 0.2%,

Polyene phosphatidylcholine 0.02% ~ 10%, is preferably 5%,

Purified water adds to 100%;

Preparation: (1) prepares burden: take lecithin, DHA, conjugated linoleic acid, arachidonic acid, polyene phosphatidylcholine by formula, add in purified water successively, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

Through laser diffraction analyzer, the Oil globule particle size distribution of this emulsion formulations is at 0.3 ~ 0.7 μm, and taste has the fragrance of oils and fats, optionally can add appropriate sweeting agent or taste modifying agent.

Embodiment 9, composite phospholipid olefin(e) acid sterol emulsion formulations and preparation method thereof

Recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%, is preferably 3%,

DHA 0.03% ~ 1%, is preferably 0.5%,

Conjugated linoleic acid 0.02% ~ 1%, is preferably 0.5%,

Arachidonic acid 0.02% ~ 1%, is preferably 0.2%,

Linolenic acid 0.02% ~ 1%, is preferably 0.2%,

Phosphatidylserine 0.02% ~ 1%, is preferably 0.2%,

Plant sterol 0.02% ~ 1%, is preferably 0.5%,

PHOSPHATIDYL ETHANOLAMINE 0.02% ~ 1%, is preferably 0.2%,

Middle long-chain fatty acid 0.5% ~ 5%, is preferably 1.5%,

Purified water adds to 100%;

Preparation: (1) prepares burden: take lecithin, DHA, conjugated linoleic acid, arachidonic acid, linolenic acid, Phosphatidylserine, plant sterol, PHOSPHATIDYL ETHANOLAMINE, middle long-chain fatty acid by formula, add successively in purified water, be settled to required volume of production, be uniformly mixed and be uniformly dispersed;

(2) emulsifying: the liquid prepared in (1) is placed in emulsion tank, nitrogen or the carbon dioxide of 25L/min is passed into continuously bottom emulsion tank, temperature of liquid be 45 ~ 55 DEG C, under the condition of speed of agitator 2800r/min, carry out emulsification procedure 20min, then interlayer cooling water is passed into, after making emulsion be cooled to 25 DEG C in 5min, stop stirring and stopping passing into nitrogen, leave standstill;

(3) homogenizing: emulsion obtained for step (2) is filtered through 500 orders, filtrate in temperature 80 ~ 90 DEG C, pressure be that 30MPa carries out homogenizing;

(5) pack, check, obtain composite phospholipid olefin(e) acid sterol emulsion formulations;

The application in following of embodiment 10, composite phospholipid DHA emulsion described in embodiment 1 to embodiment 9

Application in the healthy product preparing oral supplementation phospholipid or unsaturated fatty acid;

Application in the healthy product of preparation protection cardiovascular and cerebrovascular vessel;

Application in the healthy product of preparation blood fat reducing;

Application in the healthy product of preparation treatment fatty liver;

Application in the healthy product preparing blood pressure lowering;

In preparation to the application in the cosmetics of skin nursing and beauty treatment.

Embodiment 11, composite phospholipid DHA emulsion intercalation method of the present invention are studied

Completely according to the preferred amounts formula of embodiment 9, be prepared into emulsion according to a conventional method, the preferred amounts composite phospholipid olefin(e) acid sterol emulsion prepared with embodiment 9 carries out centrifugal, heated and boiled and thawing 5 times repeatedly respectively, the states of matter change before and after contrast operation, its stability of analysis and comparison.Result is as following table 1.

Table 1

As can be seen from Table 1, composite phospholipid DHA emulsion intercalation method of the present invention is significantly higher than conventional fat type emulsion.

The bioavailability study of embodiment 12, composite phospholipid DHA emulsion of the present invention

The present invention contains the emulsion of lecithin, DHA, other liposoluble substance and water, the little and narrow diameter distribution of Oil globule particle diameter, and close to nano-scale, the oral absorption for it utilizes, significant; Such as, be to be understood that, oral preparation needs to absorb through gastrointestinal just can enter blood of human body to reach physiological action, and the quality of oral absorption, directly affects the therapeutic effect of medicine.According to the concept of pharmaceutics, bioavailability (Bioavailability) refers to that preparation is rapidly absorbed into sanguimotor producing level and utilizes speed.Compare the preparation of other non-emulsified liquid type, the emulsion that the present invention contains lecithin, DHA, other liposoluble substance and water has excellent water-oiliness dissolubility, the oral drug preparation taking liposoluble substance as active ingredient is entered in sanguimotor degree and speed in absorption, there is significant facilitation and meaning.Preparation enters the absorption process after gastrointestinal, divide two stages, namely disintegrate or dispersion and two stages of Gastrointestinal Wall Absorption, first need to scatter in gastric juice or intestinal juice, and then contact and be attached to gastrointestinal wall and carry out absorbing and entering blood, this two stages all can affect the performance with drug effect that absorbs of medicine, liposoluble substance is prepared as water dispersible good, extra small and the emulsion of narrow diameter distribution of Oil globule particle diameter, stripping and the rate of dispersion of liposoluble substance are significantly accelerated, substantially increase degree and the rate of dispersion of its disintegrate dispersion in gastric juice or intestinal juice, then the contact of liposoluble substance and gastrointestinal wall is more abundant, be attached to Gastrointestinal Wall Absorption and to enter the active ingredient of blood also more abundant, this raising for the bioavailability of liposoluble substance is significant.

Take entirely according to the formula of embodiment 9, be prepared into according to a conventional method and emulsion, the composite phospholipid olefin(e) acid sterol emulsion prepared with the embodiment of the present invention 9, with under condition, give rat oral gavage respectively, carry out studying and compare total oleic acid of two kinds of emulsions, the bioavailability of DHA; Result of study finds, compound lecithin DHA emulsion of the present invention is compared with the made emulsion of conventional method, the bioavailability of total oleic acid improves the bioavailability raising more than 36% of more than 41%, DHA, and the raising of the highly significant of composite phospholipid DHA emulsion of the present invention in bioavailability is shown.

Claims

1. the emulsion compositions containing lecithin, DHA and water, in compositions, the content of water is greater than 70% with mass ratio range, lecithin and DHA weight ratio are 1:(0.005 ~ 1), wherein, the preparation method of described emulsion compositions comprises: lecithin, DHA mixed with water, emulsifying and make uniform liquid, and the Oil globule particle diameter in emulsion is less than 5 μm.

2. one kind contains lecithin, DHA, the emulsion compositions of other liposoluble substance and purified water, in emulsion compositions, the content of purified water is greater than 70% with mass ratio range, lecithin and DHA weight ratio are 1:(0.005 ~ 1), other liposoluble substance described comprises one or more compositions following: arachidonic acid, linolenic acid, alpha-linolenic acid, gamma-Linolenic acid, linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, Phosphatidylserine, polyene phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, phosphatidylinositols, phosphatidyl glycerol, plant sterol, plant sterol ester, phytostanol ester, middle long-chain fatty acid, wherein, the preparation method of described emulsion compositions comprises: lecithin, DHA, other liposoluble substance mixed with purified water, emulsifying and make uniform liquid, and the Oil globule particle diameter in emulsion is less than 5 μm.

3. one kind contains lecithin, DHA, the emulsion compositions of other liposoluble substance and purified water, in emulsion compositions, the content of purified water is greater than 70% with mass ratio range, lecithin and DHA weight ratio are 1:(0.005 ~ 1), other liposoluble substance described comprises one or more compositions following: arachidonic acid, linolenic acid, alpha-linolenic acid, gamma-Linolenic acid, linoleic acid, conjugated linoleic acid, conjugated linoleic acid glyceride, Phosphatidylserine, polyene phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, phosphatidylinositols, phosphatidyl glycerol, plant sterol, plant sterol ester, phytostanol ester, middle long-chain fatty acid, wherein, the preparation method of described emulsion compositions comprises:

(2) emulsifying: the liquid prepared in (1) is placed in emulsion tank, nitrogen or the carbon dioxide of 10 ~ 80L/min is passed into continuously bottom emulsion tank, temperature of liquid be 45 ~ 65 DEG C, under speed of agitator is greater than the condition of 2000r/min, carry out emulsification procedure 5 ~ 35min, then, after emulsion being cooled to 25 DEG C in 5min, stop stirring and stopping passing into nitrogen or carbon dioxide;

(3) homogenizing: emulsion obtained for step (2) is filtered through 100 ~ 500 orders, filtrate is that 15 ~ 58MPa carries out homogenizing in temperature 50 ~ 98 DEG C, pressure, and makes the Oil globule particle diameter in emulsion be less than 5 μm;

(5) pack, check, to obtain final product.

4. according to claim 1 to the emulsion compositions of any one according to claim 3, wherein

Emulsion compositions contains lecithin, DHA, arachidonic acid and purified water;

Emulsion compositions contains lecithin, DHA, linolenic acid and purified water

Emulsion compositions contains lecithin, DHA, alpha-linolenic acid and purified water;

Emulsion compositions contains lecithin, DHA, gamma-Linolenic acid and purified water;

Emulsion compositions contains lecithin, DHA, linoleic acid and purified water;

Emulsion compositions contains lecithin, DHA, conjugated linoleic acid and purified water;

Emulsion compositions contains lecithin, DHA, conjugated linoleic acid glyceride and purified water;

Emulsion compositions contains lecithin, DHA, Phosphatidylserine and purified water;

Emulsion compositions contains lecithin, DHA, polyene phosphatidylcholine and purified water;

Emulsion compositions contains lecithin, DHA, PHOSPHATIDYL ETHANOLAMINE and purified water;

Emulsion compositions contains lecithin, DHA, phosphatidylinositols and purified water;

Emulsion compositions contains lecithin, DHA, phosphatidyl glycerol and purified water;

Emulsion compositions contains lecithin, DHA, plant sterol (or plant sterol ester, or phytostanol ester) and purified water;

Emulsion compositions contains lecithin, DHA, middle long-chain fatty acid and purified water;

Containing lecithin, DHA, conjugated linoleic acid, gamma-Linolenic acid and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, conjugated linoleic acid, alpha-linolenic acid, plant sterol (or plant sterol ester, or phytostanol ester) and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, alpha-linolenic acid, middle long-chain fatty acid and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, conjugated linoleic acid, alpha-linolenic acid, middle long-chain fatty acid and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, linoleic acid, Phosphatidylserine, plant sterol (or plant sterol ester, or phytostanol ester) and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, conjugated linoleic acid and purified water in emulsion compositions;

Containing lecithin, DHA, polyene phosphatidylcholine, conjugated linoleic acid and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, polyene phosphatidylcholine, conjugated linoleic acid and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, PHOSPHATIDYL ETHANOLAMINE, conjugated linoleic acid and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, phosphatidylinositols, conjugated linoleic acid and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, phosphatidyl glycerol, conjugated linoleic acid and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, conjugated linoleic acid, middle long-chain fatty acid and purified water in emulsion compositions;

Containing lecithin, DHA, gamma-Linolenic acid, middle long-chain fatty acid and purified water in emulsion compositions;

Containing lecithin, DHA, arachidonic acid, conjugated linoleic acid, plant sterol (or plant sterol ester, or phytostanol ester), middle long-chain fatty acid and purified water in emulsion compositions;

Or, containing lecithin, DHA, arachidonic acid, gamma-Linolenic acid, conjugated linoleic acid, Phosphatidylserine, plant sterol (or plant sterol ester, or phytostanol ester) and purified water in emulsion compositions.

5. a lecithin DHA emulsion, its recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5.5%,

DHA 0.03%～1.5%，

Sucralose 0 ~ 0.05%,

Purified water adds to 100%;

(5) pack, check, to obtain final product.

6. a composite phospholipid DHA emulsion, its recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%,

DHA 0.03%～1%，

Conjugated linoleic acid 0.5% ~ 5%,

Arachidonic acid 0 ~ 3.5%,

Purified water adds to 100%;

(5) pack, check, to obtain final product.

7. a composite phospholipid DHA emulsion, its recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 8%,

DHA 0.03%～1.5%，

Peony seed oil linolenic acid (linolenic acid content >=38%) 0.5% ~ 10%(is in linolenic acid content),

Purified water adds to 100%;

(4) fill, sterilizing: the emulsion after homogenizing is carried out vacuum outgas, canned, sealing, and 115 ~ 121 DEG C of sterilizings 20 minutes; (5) pack, check, to obtain final product.

8. a composite phospholipid DHA emulsion, its recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%,

DHA 0.03%～1%，

Conjugated linoleic acid 0.5% ~ 5%,

Phosphatidylserine 0.1% ~ 5%,

Purified water adds to 100%;

(5) pack, check.

9. a composite phospholipid olefin(e) acid sterol emulsion, its recipe quantity by weight percentage, contains:

Lecithin 0.5% ~ 5%,

DHA 0.03%～1%，

Conjugated linoleic acid 0.02% ~ 1%,

Arachidonic acid 0.02% ~ 1%,

Linolenic acid 0.02% ~ 1%,

Phosphatidylserine 0.02% ~ 1%,

Plant sterol 0.02% ~ 1%,

PHOSPHATIDYL ETHANOLAMINE 0.02% ~ 1%,

Middle long-chain fatty acid 0.5% ~ 5%,

Purified water adds to 100%;

(5) pack, check, to obtain final product.

10. the application of the emulsion that contains lecithin and DHA of any one described in claim 1 to claim 9 in following:

Application in the healthy product of preparation blood fat reducing;

Application in the healthy product of preparation treatment fatty liver;

Application in the healthy product preparing blood pressure lowering;