CN104429851A - Method for transplanting Qaidam lycium barbarum in-vitro rapid propagation seedling - Google Patents
Method for transplanting Qaidam lycium barbarum in-vitro rapid propagation seedling Download PDFInfo
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- CN104429851A CN104429851A CN201410641030.0A CN201410641030A CN104429851A CN 104429851 A CN104429851 A CN 104429851A CN 201410641030 A CN201410641030 A CN 201410641030A CN 104429851 A CN104429851 A CN 104429851A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The invention relates to a method for transplanting a Qaidam lycium barbarum in-vitro rapid propagation seedling. The method for transplanting the Qaidam lycium barbarum in-vitro rapid propagation seedling comprises the following steps that (1) a Qaidam lycium barbarum regeneration plant which grows in a rooting culture medium for ten to fourteen days and has a well-developed root is taken out of the rooting culture medium, and then the culture medium adhering to the root of the plant is washed off through tap water at the room temperature, so that a clean regeneration plant is obtained; (2) the clean regeneration plant is placed in a container containing clean water for seedling hardening, and then a plant subjected to seedling hardening is obtained; (3) a seedling culture matrix is prepared; (4) the plant subjected to seedling hardening is transplanted to the seedling culture matrix by slightly clamping the one-third portion of the root of the plant with forceps, the seedling culture matrix is compacted, rooting water is poured in the seedling culture matrix, and then a transplanted rapid propagation seedling is obtained; (5) a seedling culture tray containing the transplanted rapid propagation seedling is placed in a seedling culture room, the transplanted rapid propagation seedling is covered with a film, acclimatization is conducted under the dim light condition, and then the rapid propagation seedling is moved to be under the light culture condition to be cultured according to a conventional method, so that the Qaidam lycium barbarum in-vitro rapid propagation seedling is obtained. By the adoption of the method for transplanting the Qaidam lycium barbarum in-vitro rapid propagation seedling, industrialized seedling culture efficiency can be improved.
Description
Technical field
The present invention relates to plant biotechnology field, particularly relate to the transplanting method of Qaidam matrimony vine rapid propagation in vitro seedling.
Background technology
Matrimony vine (
lycium barbarummill.) for Solanaceae (
solanaceae) Lycium (
lycium) perennial machaka, fruit claims the fruit of Chinese wolfberry, and root skin claims the root bark of Chinese wolf-berry, all can be used as medicine, and tender stem, leaf can do vegetables.The fruit of Chinese wolfberry has improving eyesight moistening lung, immunological regulation, anti-fatty liver, hypoglycemic blood fat, anti-ageing and improve effects of the ageing disorders such as the elderly's organ failure.Caidamu Basin matrimony vine aboundresources is the emerging producing region of matrimony vine, and the Qaidam matrimony vine produced is one of former plant of the famous genuine native drug in Qinghai, and " bavin Qi " is also one of domestic top quality matrimony vine.In the Caidamu Basin, plantation matrimony vine not only can regulate agricultural structure, can also improve the vegetation coverage in the Caidamu Basin, improve region microclimate, and control desertification of land, has important function to the recovery of ecological protection and degeneration ecotope.
After matrimony vine rapid propagation in vitro system is built up, the transplantation technique of regeneration plant directly has influence on the survival rate of fast propagating seedling, then affect production efficiency and the economical benefit of factorial seedling growth, therefore, setting up transplantation technique is effectively the important step realizing factorial seedling growth.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of transplanting method of Qaidam matrimony vine rapid propagation in vitro seedling of suitable factorial seedling growth.
For solving the problem, the transplanting method of Qaidam of the present invention matrimony vine rapid propagation in vitro seedling, comprises the following steps:
By growing 10 ~ 14 days in root media, the good Qaidam matrimony vine regeneration plant of root development takes out from root media, then clean the medium going root to adhere to the running water under room temperature, obtain clean regeneration plant;
(2) described clean regeneration plant is put into the container filling clean water, make its root submerge in water, cauline leaf is exposed on the water surface, places and carries out hardening in 1 ~ 3 hour, when the globule is not with on its blade face, obtain the plant after hardening under room temperature condition;
(3) prepare seedling medium: after peat soil, forest soil, fine sand being mixed according to the ratio of 2kg:1 kg:0 ~ 1kg, load in 7cm × 12cm non-woven fabrics Seedling bag and get final product;
(4) transplant first 1 day, in the seedling-cultivating tray filling 54 ~ 64 bags of described seedling mediums, add the water of 2.5 ~ 3.0L, described seedling medium is soaked completely; Plant after described hardening gently being clamped root 1/3 place with tweezers in second day transplants to described seedling medium, seedling medium described in compacting, and pours into 20 ~ 50mL normal root water, obtains the fast propagating seedling of having transplanted;
(5) the seedling-cultivating tray holding the good fast propagating seedling of described transplanting is put into that temperature is 22 ~ 25 DEG C, humidity is between the nursery of 60 ~ 70%, re-cover film, tame under the low light level after 1 ~ 2 day and remove film, then carry out according to a conventional method under moving to illumination cultivation condition cultivating and obtain Qaidam matrimony vine rapid propagation in vitro seedling, every 5 ~ 7 days of period watered a water, irrigated at every turn.
Described step (5) in illumination cultivation condition refer to that temperature is 22 ~ 25 DEG C, humidity is 60 ~ 70%, intensity of illumination 10000 ~ 30000lux, light application time 12 ~ 18 hours/day.
The present invention compared with prior art has the following advantages:
1, the present invention is by the rapid propagation in vitro seedling of acquired Qaidam matrimony vine improved seeds by committed steps such as hardening, transplanting, transplanting domestication, seedling managements, makes survival rate >=95% of transplanted seedling.
2, due to the overlay film moisturizing after hardening, transplanting of fast propagating seedling in the present invention, low light level domestication 1-2 days, fast propagating seedling adapts to cultivation condition fast, therefore, the survival rate that rapid propagation in vitro seedling is high in transplanting process can be ensured, the highest 100%(that can reach of survival rate is see Fig. 1), thus improve the efficiency of factorial praluction seedling.
3, the fast propagating seedling transplantation technique owing to setting up in the present invention, transplanted seedling can survive in the short time and enter fast growing period, shortens growing-seedling period, therefore, can provide a large amount of high quality seedling (see Fig. 2) at short notice.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is that in the present invention, matrimony vine fast propagating seedling transplants photo in seedling medium.
Fig. 2 transplants matrimony vine fast propagating seedling upgrowth situation photo after 6 weeks in the present invention.
Embodiment
embodiment 1the transplanting method of Qaidam matrimony vine rapid propagation in vitro seedling, comprises the following steps:
By growing 10 days in root media, the good Qaidam matrimony vine regeneration plant of root development takes out from root media, then clean the medium going root to adhere to the running water under room temperature, obtain clean regeneration plant.
(2) clean regeneration plant is put into the container filling clean water, make its root submerge in water, cauline leaf is exposed on the water surface, places and carries out hardening in 1 hour, when the globule is not with on its blade face, obtain the plant after hardening under room temperature condition.
(3) prepare seedling medium: after peat soil, forest soil, fine sand being mixed according to the ratio of 2kg:1 kg:0 kg, load in 7cm × 12cm non-woven fabrics Seedling bag and get final product.
(4) transplant first 1 day, in the seedling-cultivating tray filling 54 bags of described seedling mediums, add the water of 2.5L, seedling medium is soaked completely; Plant after hardening is gently clamped root 1/3 place with tweezers in second day to transplant in seedling medium, compacting seedling medium, and pour into 20mL normal root water, obtain the fast propagating seedling of having transplanted.
(5) the seedling-cultivating tray holding the fast propagating seedling of having transplanted is put into that temperature is 22 DEG C, humidity is between the nursery of 60%, re-cover film, tame under the low light level after 1 ~ 2 day and remove film, then carry out according to a conventional method under moving to illumination cultivation condition cultivating and obtain Qaidam matrimony vine rapid propagation in vitro seedling, every 5 ~ 7 days of period watered a water, irrigated at every turn.After seedling grows 4 weeks, statistics survival rate is 95.0%.
Wherein: illumination cultivation condition refers to that temperature is 22 DEG C, and humidity is 60%, intensity of illumination 10000lux, light application time 18 hours/day.
embodiment 2the transplanting method of Qaidam matrimony vine rapid propagation in vitro seedling, comprises the following steps:
By growing 14 days in root media, the good Qaidam matrimony vine regeneration plant of root development takes out from root media, then clean the medium going root to adhere to the running water under room temperature, obtain clean regeneration plant.
(2) clean regeneration plant is put into the container filling clean water, make its root submerge in water, cauline leaf is exposed on the water surface, places and carries out hardening in 3 hours, when the globule is not with on its blade face, obtain the plant after hardening under room temperature condition.
(3) prepare seedling medium: after peat soil, forest soil, fine sand being mixed according to the ratio of 2 kg:1 kg:1 kg, load in 7cm × 12cm non-woven fabrics Seedling bag and get final product.
(4) transplant first 1 day, in the seedling-cultivating tray filling 64 bags of described seedling mediums, add the water of 3.0L, seedling medium is soaked completely; Plant after hardening is gently clamped root 1/3 place with tweezers in second day to transplant in seedling medium, compacting seedling medium, and pour into 50mL normal root water, obtain the fast propagating seedling of having transplanted.
(5) the seedling-cultivating tray holding the fast propagating seedling of having transplanted is put into that temperature is 25 DEG C, humidity is between the nursery of 70%, re-cover film, tame under the low light level after 1 ~ 2 day and remove film, then carry out according to a conventional method under moving to illumination cultivation condition cultivating and obtain Qaidam matrimony vine rapid propagation in vitro seedling, every 5 ~ 7 days of period watered a water, irrigated at every turn.After seedling grows 4 weeks, statistics survival rate is 98.0%.
Wherein: illumination cultivation condition refers to that temperature is 25 DEG C, and humidity is 70%, intensity of illumination 30000lux, light application time 12 hours/day.
embodiment 3the transplanting method of Qaidam matrimony vine rapid propagation in vitro seedling, comprises the following steps:
By growing 12 days in root media, the good Qaidam matrimony vine regeneration plant of root development takes out from root media, then clean the medium going root to adhere to the running water under room temperature, obtain clean regeneration plant.
(2) clean regeneration plant is put into the container filling clean water, make its root submerge in water, cauline leaf is exposed on the water surface, places and carries out hardening in 2 hours, when the globule is not with on its blade face, obtain the plant after hardening under room temperature condition.
(3) prepare seedling medium: after peat soil, forest soil, fine sand being mixed according to the ratio of 2kg:1 kg:0.5kg, load in 7cm × 12cm non-woven fabrics Seedling bag and get final product.
(4) transplant first 1 day, in the seedling-cultivating tray filling 59 bags of described seedling mediums, add the water of 2.8L, seedling medium is soaked completely; Plant after hardening is gently clamped root 1/3 place with tweezers in second day to transplant in seedling medium, compacting seedling medium, and pour into 35mL normal root water, obtain the fast propagating seedling of having transplanted.
(5) the seedling-cultivating tray holding the fast propagating seedling of having transplanted is put into that temperature is 23 DEG C, humidity is between the nursery of 65%, re-cover film, tame under the low light level after 1 ~ 2 day and remove film, then carry out according to a conventional method under moving to illumination cultivation condition cultivating and obtain Qaidam matrimony vine rapid propagation in vitro seedling, every 5 ~ 7 days of period watered a water, irrigated at every turn.Seedling adds up survival rate after growing 4 weeks, reaches as high as 100%.
Wherein: illumination cultivation condition refers to that temperature is 23 DEG C, and humidity is 65%, intensity of illumination 20000lux, light application time 15 hours/day.
Claims (2)
1. the transplanting method of Qaidam matrimony vine rapid propagation in vitro seedling, comprises the following steps:
By growing 10 ~ 14 days in root media, the good Qaidam matrimony vine regeneration plant of root development takes out from root media, then clean the medium going root to adhere to the running water under room temperature, obtain clean regeneration plant;
(2) described clean regeneration plant is put into the container filling clean water, make its root submerge in water, cauline leaf is exposed on the water surface, places and carries out hardening in 1 ~ 3 hour, when the globule is not with on its blade face, obtain the plant after hardening under room temperature condition;
(3) prepare seedling medium: after peat soil, forest soil, fine sand being mixed according to the ratio of 2kg:1 kg:0 ~ 1kg, load in 7cm × 12cm non-woven fabrics Seedling bag and get final product;
(4) transplant first 1 day, in the seedling-cultivating tray filling 54 ~ 64 bags of described seedling mediums, add the water of 2.5 ~ 3.0L, described seedling medium is soaked completely; Plant after described hardening gently being clamped root 1/3 place with tweezers in second day transplants to described seedling medium, seedling medium described in compacting, and pours into 20 ~ 50mL normal root water, obtains the fast propagating seedling of having transplanted;
(5) the seedling-cultivating tray holding the good fast propagating seedling of described transplanting is put into that temperature is 22 ~ 25 DEG C, humidity is between the nursery of 60 ~ 70%, re-cover film, tame under the low light level after 1 ~ 2 day and remove film, then carry out according to a conventional method under moving to illumination cultivation condition cultivating and obtain Qaidam matrimony vine rapid propagation in vitro seedling, every 5 ~ 7 days of period watered a water, irrigated at every turn.
2. transplanting method as claimed in claim 1, is characterized in that: described step (5) in illumination cultivation condition refer to that temperature is 22 ~ 25 DEG C, humidity is 60 ~ 70%, intensity of illumination 10000 ~ 30000lux, light application time 12 ~ 18 hours/day.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410641030.0A CN104429851B (en) | 2014-11-14 | 2014-11-14 | The method for transplanting of Qaidam Fructus Lycii rapid propagation in vitro Seedling |
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| CN201410641030.0A CN104429851B (en) | 2014-11-14 | 2014-11-14 | The method for transplanting of Qaidam Fructus Lycii rapid propagation in vitro Seedling |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583407A (en) * | 2019-10-18 | 2019-12-20 | 青岛市农业科学研究院 | Radish seed-replacing and reserving method |
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| KR20130091385A (en) * | 2012-02-08 | 2013-08-19 | 재단법인 진안홍삼연구소 | Red ginseng functional beverage comprising panax ginseng concentrate and fruit concentrate |
| CN103299793A (en) * | 2012-03-09 | 2013-09-18 | 赵利刚 | Cutting propagation method of northern Lycium chinense |
| CN103609451A (en) * | 2013-11-29 | 2014-03-05 | 山东省农作物种质资源中心 | Medlar sterile seedling and method for obtaining induced healing of medlar sterile seedling |
| CN103843664A (en) * | 2014-03-24 | 2014-06-11 | 甘肃农业大学 | Lycium exsertum tissue culture and rapid propagation method |
| CN104054564A (en) * | 2014-06-12 | 2014-09-24 | 中宁县沃杞农业科技有限公司 | Medlar virus-free tissue culture seedling transplanting method |
| CN104094841A (en) * | 2014-03-24 | 2014-10-15 | 甘肃农业大学 | Tissue culture and rapid propagation method of solanaceae lycium brevipes |
-
2014
- 2014-11-14 CN CN201410641030.0A patent/CN104429851B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000057690A2 (en) * | 1999-03-25 | 2000-10-05 | University Of Guelph | Micropropagation and production of phytopharmaceutical plants |
| KR20130091385A (en) * | 2012-02-08 | 2013-08-19 | 재단법인 진안홍삼연구소 | Red ginseng functional beverage comprising panax ginseng concentrate and fruit concentrate |
| CN103299793A (en) * | 2012-03-09 | 2013-09-18 | 赵利刚 | Cutting propagation method of northern Lycium chinense |
| CN103181324A (en) * | 2013-03-23 | 2013-07-03 | 甘肃农业大学 | Method for rapidly propagating high-quality seedlings of Lycium ruthenicum Murr. |
| CN103609451A (en) * | 2013-11-29 | 2014-03-05 | 山东省农作物种质资源中心 | Medlar sterile seedling and method for obtaining induced healing of medlar sterile seedling |
| CN103843664A (en) * | 2014-03-24 | 2014-06-11 | 甘肃农业大学 | Lycium exsertum tissue culture and rapid propagation method |
| CN104094841A (en) * | 2014-03-24 | 2014-10-15 | 甘肃农业大学 | Tissue culture and rapid propagation method of solanaceae lycium brevipes |
| CN104054564A (en) * | 2014-06-12 | 2014-09-24 | 中宁县沃杞农业科技有限公司 | Medlar virus-free tissue culture seedling transplanting method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583407A (en) * | 2019-10-18 | 2019-12-20 | 青岛市农业科学研究院 | Radish seed-replacing and reserving method |
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