CN103181324A - Method for rapidly propagating high-quality seedlings of Lycium ruthenicum Murr. - Google Patents

Abstract

The invention relates to a method for rapidly propagating high-quality seedlings of Lycium ruthenicum Murr.. The method includes the steps as follows: firstly, seeds are washed with running water, sterilized with 75% (vol) alcohol and washed with aseptic water for 3 to 4 times; then, the seeds are sterilized with 0.1% (mass) mercuric chloride, washed with sterilized water for 3 to 4 times, and then inoculated in an MS culture medium to cultivate aseptic seedlings; and after the aseptic seedlings are cultivated for one month, the stems of the aseptic seedlings are cut off, and then cut into stem segments with the lengths of 0.5 cm and inoculated into a culture medium containing MSKH2PO413.5mg/LNAA0.1mg/L for cultivation. The method has the advantages that all the sterile seedling can take root, the aseptic seedlings of Lycium ruthenicum Murr. can be rapidly propagated, the period is short, the growth of the seedling is not influenced by factors of seasons, climate, natural disasters and the like, and the industrial production can be achieved; and by means of the technology, a plurality of problems of low propagation capacity, lack in resource, agriculture chemical residues and the like of a medicinal plant can be solved.

Description

The black fruit lyceum high quality seedling is the method for breeding fast

Technical field

The present invention relates to a kind of black fruit lyceum breeding method, more specifically to the method for the quick breeding of a kind of black fruit lyceum high quality seedling.

Background technology

Black fruit lyceum ( Lycium ruthenicumMurr.) be the Solanaceae matrimony vine, belong to a kind of salt crude drug and use plant, mainly be distributed in provinces and regions such as Ningxia, Shanxi, Gansu, Qinghai, Xinjiang, Tibet in China.Black fruit lyceum is of many uses, contains multiple human body essential amino acid and trace element in its fruit, can improve the human immunological competence; Good, the strong coloring force of the black fruit lyceum pigment stability that from fruit juice, extracts, it is the desirable edible natural anthocyanin of domestic urgent need, not only can be applicable in medicine and the food, and can be used as natural dye and be applied to light and textile industries, is to find the wild plant that procyanidin content is the highest up to now.Simultaneously, black fruit lyceum has drought resisting, cold-resistant, salt tolerant alkali, root turion is strong and plurality of advantages such as anti-soil depletion, is saline land improvement, bank protection, the good seeds that prevent erosion.Therefore, no matter black fruit lyceum still ecological environment treatment aspect all has widely market prospects and potentiality to be exploited in the economic product exploitation, can become the important economic tree of medicines and health protection, economic forest construction and saline land biological control.At present, the research overwhelming majority of black fruit lyceum concentrates on its physiological ecological, analysis of effective component, development and use and administers aspects such as desertification and wild provenance domestication, and reports for work seldom about the cultivation of black fruit lyceum tissue and the research of quick proliferation.Great this grade of benevolence tower (2005) utilizes the stem section of band axillalry bud black fruit lyceum to be carried out differentiation is taken root and the callus induction adventitious bud proliferation is studied, and the research of the quick proliferation of relevant black fruit lyceum high quality seedling yet there are no report.The Gansu Province's main area of black fruit lyceum wild resource natural conditions are abominable at present, and vegetation degeneration is serious, even dead in flakes, and the normal existence to black fruit lyceum has caused serious threat, is in Critical Condition substantially.

Summary of the invention

The technical problem to be solved in the present invention is: the problem that solves the breeding of our province black fruit lyceum breed breeding and breeding; solve long problem of seminal propagation cycle; a kind of black fruit lyceum high quality seedling method of breeding fast is provided; obtain high quality seedling; shorten the black fruit lyceum breeding cycle, accelerate black fruit lyceum stock breeding process, can carry out batch production large-scale production; satisfy market to the demand of black fruit lyceum, protect wild black fruit lyceum natural resources and good natural ecological environment.

The present invention realizes by following sequential steps:

A, at first select the black fruit lyceum high quality seed with flowing water flushing 2～3h;

B, the seed after will washing be with 75% alcohol disinfecting 1min, then with the seed behind the alcohol disinfecting with aseptic water washing 3～4 times;

C, with the seed behind the aseptic water washing again with 0.1% mercuric chloride sterilization 5min, then with the seed after the mercuric chloride sterilization with aseptic water washing 3～4 times;

D, will be inoculated in through the seed of sterilization treatment in the MS medium and cultivate aseptic seedling at last, cultivation temperature is 24 ℃～26 ℃, and intensity of illumination is 1500 LX～2000LX, and light application time is 24h/d;

E, cultivation after 1 month are cut the cane of aseptic seedling, are cut into the stem section of 0.5cm length, insert MS+ KH under aseptic condition ₂PO ₄13.5mg/L carry out the numerous cultivation of quick expansion of aseptic seedling in the+NAA 0.1mg/L medium, intensity of illumination is 2000LX, light application time is 12h/d～16h/d.

The present invention utilizes method for tissue culture to breed the cane of black fruit lyceum aseptic seedling fast, and its advantage is: make the aseptic seedling rooting rate reach 100%, can breed the aseptic nursery stock of black fruit lyceum fast.Cycle is short, is not subjected to the influence of factors such as season, weather, natural calamity, and can realizes batch production production.Utilize this technology can solve problems such as the fertility of medicinal plant is low, scarcity of resources, residue of pesticide.Our Gansu is to adopt plant tissue culture technique first on the other hand; this breeds fast to Gansu black fruit lyceum new germ plasm resource; not only can meet the need of market, but also check winds and fix drifting sand, preserving the ecological environment and protecting aspects such as germ plasm resource to have strategic importance.

Embodiment

Embodiment 1

The present invention has following sequential steps:

A, at first select the black fruit lyceum high quality seed with flowing water flushing 2～3h;

B, the seed after will washing be with 75% alcohol disinfecting 1min, then with the seed behind the alcohol disinfecting with aseptic water washing 3～4 times;

C, with the seed behind the aseptic water washing again with 0.1% mercuric chloride sterilization 5min, then with the seed after the mercuric chloride sterilization with aseptic water washing 3～4 times;

D, will be inoculated in through the seed of sterilization treatment in the MS medium and cultivate aseptic seedling at last, cultivation temperature is 24 ℃, and intensity of illumination is 1500 LX, and light application time is 24h/d;

E, cultivation after 1 month are cut the cane of aseptic seedling, are cut into the stem section of 0.5cm length, insert MS+ KH under aseptic condition ₂PO ₄13.5mg/L proceed the numerous cultivation of quick expansion of aseptic seedling in the+NAA 0.1mg/L medium, intensity of illumination is 2000LX, light application time is 12h/d.

Embodiment 2

Embodiment 2 is identical with the method for embodiment 1, and difference is that the cultivation temperature of aseptic seedling in the steps d is 25 ℃, and intensity of illumination is 1800LX.Light application time is 14h/d among the step e.

Embodiment 3

Embodiment 3 is identical with the method for embodiment 1, and difference is that the cultivation temperature of aseptic seedling in the steps d is 26 ℃, and intensity of illumination is 2000LX.Light application time is 16h/d among the step e.

In sum: this technology makes the aseptic seedling rooting rate reach 100%, can breed the aseptic nursery stock of black fruit lyceum fast.Gansu is to adopt the quick multiplication technique of black fruit lyceum aseptic seedling cane first; this breeds fast to Gansu black fruit lyceum new germ plasm resource; not only can meet the need of market, but also check winds and fix drifting sand, preserving the ecological environment and protecting aspects such as germplasm to have strategic importance.

Claims (1)

1. the black fruit lyceum high quality seedling method of breeding fast, this method has following sequential steps:

A, at first select the black fruit lyceum high quality seed with flowing water flushing 2～3h;

B, the seed after will washing be with 75% alcohol disinfecting 1min, then with the seed behind the alcohol disinfecting with aseptic water washing 3～4 times;

C, with the seed behind the aseptic water washing again with 0.1% mercuric chloride sterilization 5min, then with the seed after the mercuric chloride sterilization with aseptic water washing 3～4 times;

D, will be inoculated in through the seed of sterilization treatment in the MS medium and cultivate aseptic seedling at last, cultivation temperature is 24 ℃～26 ℃, and intensity of illumination is 1500 LX～2000LX, and light application time is 24h/d;

E, cultivation after 1 month are cut the cane of aseptic seedling, are cut into the stem section of 0.5cm length, insert MS+ KH under aseptic condition ₂PO ₄13.5mg/L carry out the numerous cultivation of quick expansion of aseptic seedling in the+NAA 0.1mg/L medium, intensity of illumination is 2000LX, light application time is 12h/d～16h/d.