CN104429851B - The method for transplanting of Qaidam Fructus Lycii rapid propagation in vitro Seedling - Google Patents
The method for transplanting of Qaidam Fructus Lycii rapid propagation in vitro Seedling Download PDFInfo
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- CN104429851B CN104429851B CN201410641030.0A CN201410641030A CN104429851B CN 104429851 B CN104429851 B CN 104429851B CN 201410641030 A CN201410641030 A CN 201410641030A CN 104429851 B CN104429851 B CN 104429851B
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- seedling
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- fructus lycii
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
Abstract
The present invention relates to the method for transplanting of a kind of Qaidam Fructus Lycii rapid propagation in vitro Seedling, the method comprises the following steps: (1) by root media grows 10 ~ 14 days, the good Qaidam Fructus Lycii regeneration plant of root development takes out from root media, then clean the culture medium going root to adhere to the tap water under room temperature, obtain clean regeneration plant；(2) clean regeneration plant is put into and the container filling clean water carries out seedling exercising, obtain the plant after seedling exercising；(3) prepare seedling medium；(4) with tweezers, the plant after seedling exercising is gently clamped root 1/3 place to transplant in described seedling medium, compacting seedling medium, and pour into and determine root water, obtain the fast propagating seedling transplanted；(5) the seedlings nursing plate holding the fast propagating seedling transplanted is put between nursery, cover film, tame under the low light level, carry out according to a conventional method when then moving to illumination cultivation cultivating and obtain Qaidam Fructus Lycii rapid propagation in vitro Seedling.The present invention can improve the efficiency of factorial praluction seedling.
Description
Technical field
The present invention relates to plant biotechnology field, particularly relate to the method for transplanting of Qaidam Fructus Lycii rapid propagation in vitro Seedling.
Background technology
Fructus Lycii (LyciumbarbarumMill.) is Solanaceae (Solanaceae) Lycium (Lycium) perennial machaka, and fruit claims Fructus Lycii, and root bark claims Cortex Lycii, all can be used as medicine, and tender stem, leaf can do vegetable.Fructus Lycii has improving eyesight lung moistening, immunomodulating, anti-fatty liver, blood sugar lowering blood fat, defying age and improves effect of the ageing disorders such as old people's organ failure.Caidamu Basin Fructus Lycii aboundresources, is the emerging producing region of Fructus Lycii, and the Qaidam Fructus Lycii produced is one of famous former plant of genuine native drug in Qinghai, and " bavin Qi " is also one of domestic top quality Fructus Lycii.Plant Fructus Lycii to be possible not only to regulate agricultural structure in the Caidamu Basin, it is also possible to improve the vegetation coverage in the Caidamu Basin, improve region miniclimate, prevent and treat desertification of land, the recovery of ecological protection and degeneration ecological environment is had important function.
After Fructus Lycii rapid propagation in vitro system is built up, the transplantation technique of regeneration plant directly influences the survival rate of fast propagating seedling, then affecting production efficiency and the economical benefit of industrial seedling rearing, therefore, setting up transplantation technique effectively is the important step realizing industrial seedling rearing.
Summary of the invention
The technical problem to be solved is to provide the method for transplanting of the Qaidam Fructus Lycii rapid propagation in vitro Seedling of a kind of suitable industrial seedling rearing.
For solving the problems referred to above, the method for transplanting of Qaidam of the present invention Fructus Lycii rapid propagation in vitro Seedling, comprise the following steps:
By root media grows 10 ~ 14 days, the good Qaidam Fructus Lycii regeneration plant of root development takes out from root media, then clean the culture medium going root to adhere to the tap water under room temperature, obtain cleaning regeneration plant；
(2) described clean regeneration plant is put in the container filling clean water so that it is root submerges in water, and stem and leaf is exposed on the water surface, place under room temperature condition and carry out seedling exercising in 1 ~ 3 hour, when its blade face is without the globule, obtain the plant after seedling exercising；
(3) prepare seedling medium: by peat soil, sylvogenic soil, fine sand according to, after the ratio mix homogeneously of 2kg:1kg:0 ~ 1kg, loading in 7cm × 12cm non-woven fabrics Seedling bag and get final product；
(4) transplant first 1 day, the seedlings nursing plate filling 54 ~ 64 bags of described seedling mediums adds the water of 2.5 ~ 3.0L, makes described seedling medium soak completely；With tweezers, the plant after described seedling exercising was gently clamped root 1/3 place in second day to transplant in described seedling medium, seedling medium described in compacting, and pour into 20 ~ 50mL and determine root water, obtain the fast propagating seedling transplanted；
(5) the seedlings nursing plate holding the good fast propagating seedling of described transplanting is put into temperature be 22 ~ 25 DEG C, humidity be 60 ~ 70% nursery between in, cover film again, thin film is removed after taming 1 ~ 2 day under the low light level, carry out according to a conventional method when then moving to illumination cultivation cultivating and obtain Qaidam Fructus Lycii rapid propagation in vitro Seedling, every 5 ~ 7 days of period watered a water, irrigated every time.
Described step (5) in illumination cultivation condition refer to that temperature is 22 ~ 25 DEG C, humidity is 60 ~ 70%, intensity of illumination 10000 ~ 30000lux, light application time 12 ~ 18 hour/day.
The present invention compared with prior art has the advantage that
1, the present invention is by the rapid propagation in vitro Seedling of acquired Qaidam Fructus Lycii improved seeds by committed steps such as seedling exercising, transplanting, transplanting domestication, seedling managements, makes survival rate >=95% of transplanted seedling.
2, due to fast propagating seedling overlay film moisturizing after seedling exercising, transplanting, low light level domestication 1-2 days in the present invention, fast propagating seedling rapidly adapts to cultivation condition, therefore, ensure that the survival rate that rapid propagation in vitro Seedling is high in transplanting process, the highest 100%(that can reach of survival rate is referring to Fig. 1), thus improving the efficiency of factorial praluction seedling.
3, the fast propagating seedling transplantation technique owing to setting up in the present invention, transplanted seedling can survive and enter fast growing period in the short time, shortens growing-seedling period, therefore, it can provide a large amount of high quality seedling (referring to Fig. 2) at short notice.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is that in the present invention, Fructus Lycii fast propagating seedling transplants photo in seedling medium.
Fig. 2 is Fructus Lycii fast propagating seedling upgrowth situation photo after transplanting in the present invention 6 weeks.
Detailed description of the invention
The method for transplanting of embodiment 1 Qaidam Fructus Lycii rapid propagation in vitro Seedling, comprises the following steps:
By root media grows 10 days, the good Qaidam Fructus Lycii regeneration plant of root development takes out from root media, then clean the culture medium going root to adhere to the tap water under room temperature, obtain cleaning regeneration plant.
(2) clean regeneration plant is put in the container filling clean water so that it is root submerges in water, and stem and leaf is exposed on the water surface, place under room temperature condition and carry out seedling exercising in 1 hour, when its blade face is without the globule, obtain the plant after seedling exercising.
(3) prepare seedling medium: by peat soil, sylvogenic soil, fine sand according to, after the ratio mix homogeneously of 2kg:1kg:0kg, loading in 7cm × 12cm non-woven fabrics Seedling bag and get final product.
(4) transplant first 1 day, the seedlings nursing plate filling 54 bags of described seedling mediums adds the water of 2.5L, makes seedling medium soak completely；With tweezers, the plant after seedling exercising was gently clamped root 1/3 place in second day to transplant in seedling medium, compacting seedling medium, and pour into 20mL and determine root water, obtain the fast propagating seedling transplanted.
(5) the seedlings nursing plate holding the fast propagating seedling transplanted is put into temperature be 22 DEG C, humidity be 60% nursery between in, cover film again, thin film is removed after taming 1 ~ 2 day under the low light level, carry out according to a conventional method when then moving to illumination cultivation cultivating and obtain Qaidam Fructus Lycii rapid propagation in vitro Seedling, every 5 ~ 7 days of period watered a water, irrigated every time.After Seedling grows 4 weeks, statistics survival rate is 95.0%.
Wherein: illumination cultivation condition refers to that temperature is 22 DEG C, and humidity is 60%, intensity of illumination 10000lux, light application time 18 hour/day.
The method for transplanting of embodiment 2 Qaidam Fructus Lycii rapid propagation in vitro Seedling, comprises the following steps:
By root media grows 14 days, the good Qaidam Fructus Lycii regeneration plant of root development takes out from root media, then clean the culture medium going root to adhere to the tap water under room temperature, obtain cleaning regeneration plant.
(2) clean regeneration plant is put in the container filling clean water so that it is root submerges in water, and stem and leaf is exposed on the water surface, place under room temperature condition and carry out seedling exercising in 3 hours, when its blade face is without the globule, obtain the plant after seedling exercising.
(3) prepare seedling medium: by peat soil, sylvogenic soil, fine sand according to, after the ratio mix homogeneously of 2kg:1kg:1kg, loading in 7cm × 12cm non-woven fabrics Seedling bag and get final product.
(4) transplant first 1 day, the seedlings nursing plate filling 64 bags of described seedling mediums adds the water of 3.0L, makes seedling medium soak completely；With tweezers, the plant after seedling exercising was gently clamped root 1/3 place in second day to transplant in seedling medium, compacting seedling medium, and pour into 50mL and determine root water, obtain the fast propagating seedling transplanted.
(5) the seedlings nursing plate holding the fast propagating seedling transplanted is put into temperature be 25 DEG C, humidity be 70% nursery between in, cover film again, thin film is removed after taming 1 ~ 2 day under the low light level, carry out according to a conventional method when then moving to illumination cultivation cultivating and obtain Qaidam Fructus Lycii rapid propagation in vitro Seedling, every 5 ~ 7 days of period watered a water, irrigated every time.After Seedling grows 4 weeks, statistics survival rate is 98.0%.
Wherein: illumination cultivation condition refers to that temperature is 25 DEG C, and humidity is 70%, intensity of illumination 30000lux, light application time 12 hour/day.
The method for transplanting of embodiment 3 Qaidam Fructus Lycii rapid propagation in vitro Seedling, comprises the following steps:
By root media grows 12 days, the good Qaidam Fructus Lycii regeneration plant of root development takes out from root media, then clean the culture medium going root to adhere to the tap water under room temperature, obtain cleaning regeneration plant.
(2) clean regeneration plant is put in the container filling clean water so that it is root submerges in water, and stem and leaf is exposed on the water surface, place under room temperature condition and carry out seedling exercising in 2 hours, when its blade face is without the globule, obtain the plant after seedling exercising.
(3) prepare seedling medium: by peat soil, sylvogenic soil, fine sand according to, after the ratio mix homogeneously of 2kg:1kg:0.5kg, loading in 7cm × 12cm non-woven fabrics Seedling bag and get final product.
(4) transplant first 1 day, the seedlings nursing plate filling 59 bags of described seedling mediums adds the water of 2.8L, makes seedling medium soak completely；With tweezers, the plant after seedling exercising was gently clamped root 1/3 place in second day to transplant in seedling medium, compacting seedling medium, and pour into 35mL and determine root water, obtain the fast propagating seedling transplanted.
(5) the seedlings nursing plate holding the fast propagating seedling transplanted is put into temperature be 23 DEG C, humidity be 65% nursery between in, cover film again, thin film is removed after taming 1 ~ 2 day under the low light level, carry out according to a conventional method when then moving to illumination cultivation cultivating and obtain Qaidam Fructus Lycii rapid propagation in vitro Seedling, every 5 ~ 7 days of period watered a water, irrigated every time.Seedling adds up survival rate after growing 4 weeks, reaches as high as 100%.
Wherein: illumination cultivation condition refers to that temperature is 23 DEG C, and humidity is 65%, intensity of illumination 20000lux, light application time 15 hour/day.
Claims (2)
1. the method for transplanting of Qaidam Fructus Lycii rapid propagation in vitro Seedling, comprises the following steps:
By root media grows 10 ~ 14 days, the good Qaidam Fructus Lycii regeneration plant of root development takes out from root media, then clean the culture medium going root to adhere to the tap water under room temperature, obtain cleaning regeneration plant；
(2) described clean regeneration plant is put in the container filling clean water so that it is root submerges in water, and stem and leaf is exposed on the water surface, place under room temperature condition and carry out seedling exercising in 1 ~ 3 hour, when its blade face is without the globule, obtain the plant after seedling exercising；
(3) prepare seedling medium: by peat soil, sylvogenic soil, fine sand according to, after the ratio mix homogeneously of 2kg:1kg:0 ~ 1kg, loading in 7cm × 12cm non-woven fabrics Seedling bag and get final product；
(4) transplant first 1 day, the seedlings nursing plate filling 54 ~ 64 bags of described seedling mediums adds the water of 2.5 ~ 3.0L, makes described seedling medium soak completely；With tweezers, the plant after described seedling exercising was gently clamped root 1/3 place in second day to transplant in described seedling medium, seedling medium described in compacting, and pour into 20 ~ 50mL and determine root water, obtain the fast propagating seedling transplanted；
(5) the seedlings nursing plate holding the good fast propagating seedling of described transplanting is put into temperature be 22 ~ 25 DEG C, humidity be 60 ~ 70% nursery between in, cover film again, thin film is removed after taming 1 ~ 2 day under the low light level, carry out according to a conventional method when then moving to illumination cultivation cultivating and obtain Qaidam Fructus Lycii rapid propagation in vitro Seedling, every 5 ~ 7 days of period watered a water, irrigated every time.
2. method for transplanting as claimed in claim 1, it is characterised in that: described step (5) in illumination cultivation condition refer to that temperature is 22 ~ 25 DEG C, humidity is 60 ~ 70%, intensity of illumination 10000 ~ 30000lux, light application time 12 ~ 18 hour/day.
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| WO2000057690A2 (en) * | 1999-03-25 | 2000-10-05 | University Of Guelph | Micropropagation and production of phytopharmaceutical plants |
| CN103181324A (en) * | 2013-03-23 | 2013-07-03 | 甘肃农业大学 | Method for rapidly propagating high-quality seedlings of Lycium ruthenicum Murr. |
| KR20130091385A (en) * | 2012-02-08 | 2013-08-19 | 재단법인 진안홍삼연구소 | Red ginseng functional beverage comprising panax ginseng concentrate and fruit concentrate |
| CN103299793A (en) * | 2012-03-09 | 2013-09-18 | 赵利刚 | Cutting propagation method of northern Lycium chinense |
| CN103609451A (en) * | 2013-11-29 | 2014-03-05 | 山东省农作物种质资源中心 | Medlar sterile seedling and method for obtaining induced healing of medlar sterile seedling |
| CN103843664A (en) * | 2014-03-24 | 2014-06-11 | 甘肃农业大学 | Lycium exsertum tissue culture and rapid propagation method |
| CN104054564A (en) * | 2014-06-12 | 2014-09-24 | 中宁县沃杞农业科技有限公司 | Medlar virus-free tissue culture seedling transplanting method |
| CN104094841A (en) * | 2014-03-24 | 2014-10-15 | 甘肃农业大学 | Tissue culture and rapid propagation method of solanaceae lycium brevipes |
-
2014
- 2014-11-14 CN CN201410641030.0A patent/CN104429851B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000057690A2 (en) * | 1999-03-25 | 2000-10-05 | University Of Guelph | Micropropagation and production of phytopharmaceutical plants |
| KR20130091385A (en) * | 2012-02-08 | 2013-08-19 | 재단법인 진안홍삼연구소 | Red ginseng functional beverage comprising panax ginseng concentrate and fruit concentrate |
| CN103299793A (en) * | 2012-03-09 | 2013-09-18 | 赵利刚 | Cutting propagation method of northern Lycium chinense |
| CN103181324A (en) * | 2013-03-23 | 2013-07-03 | 甘肃农业大学 | Method for rapidly propagating high-quality seedlings of Lycium ruthenicum Murr. |
| CN103609451A (en) * | 2013-11-29 | 2014-03-05 | 山东省农作物种质资源中心 | Medlar sterile seedling and method for obtaining induced healing of medlar sterile seedling |
| CN103843664A (en) * | 2014-03-24 | 2014-06-11 | 甘肃农业大学 | Lycium exsertum tissue culture and rapid propagation method |
| CN104094841A (en) * | 2014-03-24 | 2014-10-15 | 甘肃农业大学 | Tissue culture and rapid propagation method of solanaceae lycium brevipes |
| CN104054564A (en) * | 2014-06-12 | 2014-09-24 | 中宁县沃杞农业科技有限公司 | Medlar virus-free tissue culture seedling transplanting method |
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