CN104399012B - A kind of Chinese medicine composition for primary liver cancer - Google Patents

Abstract

The present invention provides a kind of Chinese medicine composition for treating primary carcinoma of liver, is made up of following Chinese medicine material, radix scutellariae, the root of herbaceous peony, fritillaria thunbergii, radix bupleuri, moutan bark, stir-baked FRUCTUS GARDENIAE, Poria cocos, the bighead atractylodes rhizome, the bark of official magnolia, dried orange peel, semen armeniacae amarae, root tuber of aromatic turmeric, radix glycyrrhizae.It using Chinese tradition Chinese herbal medicine is raw material that this Chinese medicine composition, which is, is formulated by the prescription of science, and by the checking repeatedly of laboratory research and clinical trial.Treatment to primary carcinoma of liver has the effect of fine, can effectively extend the life cycle of patient, prevent from recurring, improve the quality of living, and obtains the purpose of long-term health existence.Medicine in this composition is without any side effects, and efficient high, medical expense is low, is easily accepted by patients.

Description

A kind of Chinese medicine composition for primary liver cancer

Technical field

The present invention relates to the field of Chinese medicines, more particularly to a kind of Chinese medicine composition for primary liver cancer.

Background technology

Primary carcinoma of liver (primary carcinoma of the liver) is one of China's common cancer, dead Rate is high, be only second in mortality of malignant tumors cis-position stomach, esophagus and occupy the 3rd and second then accounted in Electric Field At Ground In Partial Region, be only second to stomach Cancer.China dies from the people of liver cancer about 110,000 every year, accounts for the 45% of whole world PLC mortality number.Initial symptoms and unobvious, late period Be mainly shown as hepatodynia, it is weak, become thin, the symptom such as jaundice, ascites.Clinically typically take surgical operation, radiotherapy, chemotherapy, life The treatment method that thing treatment, targeted therapy are combined with Chinese medicine.Patients with terminal often spreads because of cancer cell, therefore cure rate is lower.

Traditional medicine think cancer be that positive unsaturated vapor, the stagnation of the circulation of vital energy, phlegm is solidifying and blood stasis caused by, therefore the treatment of cancer will be with " softening and resolving hard mass " is principle, with extending life, palliates the agonizing sufferings, prevents from recurring and shifts, final to realize what is " survived for a long time with knurl " Purpose.On tcm clinical practice, liver cancer belongs to the categories such as " liver product ", " mass at the right hypochondrium ", " tympanites ", " jaundice " more.Traditional medicine thinks feelings Will is depressed, functional activity of QI being not smooth, liver dysfunction, therefore sees upper abdomen distending pain, and stomach receives decline, and tongue is greasy, small and wiry pulse；Qi stagnation blood stasis, courage and uprightness are obstructed, Accumulate over a long period, therefore have product under seeing rib, distending pain is uncomfortable, lassitude hypodynamia, darkish complexion, becomes thin, tongue is greasy, purplish tongue, thready and hesitant pulse；Spleen Void life is wet, wet strongly fragrantization heat, accumulates in heat toxin, therefore sees jaundice, generates heat, bleeding from the gum；Tympanites, yellow and greasy fur and feel, wiry and frequent pulse.Chinese medicine is explored to control The new way of liver cancer is treated, is the emphasis of current medical personnel concern, and where the hope that makes a breakthrough of liver cancer treatment.

The method for the treatment of late primary liver cancer has at present：Supporting treatment, chemotherapy, biological therapy, targeted therapy and Chinese medicine. But conditions of patients development is still uncontrollable, transfer and progress are still this sick subject matter.At present, use is distinguished more on tcm clinical practice The treatment method treated is demonstrate,proved, is specifically included：1) invigorating the spleen qi-regulating；2) nourishing yin and nourishing blood；3) internal and external application；4) it is clearing heat and detoxicating；5) invigorate blood circulation Dissolving stasis.There is course for the treatment of length, slowly effect, DeGrain in these drug treatments, the shortcomings of being not easy to be easily accepted by the patient.Make a general survey of Each report of Chinese traditional treatment liver cancer, most of therapeutic effect are difficult to reach treatment of the World Health Organization (WHO) on solid tumor The complete incidence graph imitated in evaluation criteria is alleviated with part, and complete incidence graph is that tumor disappearance maintains more than 4 weeks, and part alleviation is tumour Vertical diameter reduces more than 50% with transverse diameter product, and maintains 4 weeks.

The content of the invention

The purpose of the present invention is to provide a kind of Chinese medicine composition for treating primary carcinoma of liver, this Chinese medicine group for problem above Compound can reach the effect treated both principal and secondary aspect of disease to primary carcinoma of liver, be finally reached long-term control disease, even up to cure liver cancer Purpose.

To solve the above problems, the technical solution adopted in the present invention is：

A kind of composition for treating primary carcinoma of liver, the composition include the Chinese herb of following weight parts：

Radix scutellariae 15-40 parts, root of herbaceous peony 15-40 parts, fritillaria thunbergii 20-50 parts, radix bupleuri 15-40 parts, moutan bark 10-40 parts, Stir-baked FRUCTUS GARDENIAE 10-35 parts, Poria cocos 10-30 parts, bighead atractylodes rhizome 10-30 parts, bark of official magnolia 10-30 parts, dried orange peel 6-30 parts, semen armeniacae amarae 6-30 Part, root tuber of aromatic turmeric 20-50 parts, radix glycyrrhizae 3-15 parts.

As preference：

The raw material dosage is as follows：Radix scutellariae 20-30 parts, root of herbaceous peony 20-30 parts, fritillaria thunbergii 25-40 parts, radix bupleuri 20-30 Part, moutan bark 20-30 parts, stir-baked FRUCTUS GARDENIAE 20-30 parts, Poria cocos 15-25 parts, bighead atractylodes rhizome 15-25 parts, bark of official magnolia 15-25 parts, dried orange peel 12-24 parts, semen armeniacae amarae 12-24 parts, root tuber of aromatic turmeric 25-40 parts, radix glycyrrhizae 6-12 parts.

The raw material dosage is as follows：20 parts of radix scutellariae, 15 parts of the root of herbaceous peony, 30 parts of fritillaria thunbergii, 25 parts of radix bupleuri, 25 parts of moutan bark, Jiao 25 parts of cape jasmine, 20 parts of Poria cocos, 20 parts of the bighead atractylodes rhizome, 20 parts of the bark of official magnolia, 20 parts of dried orange peel, 18 parts of semen armeniacae amarae, 30 parts of root tuber of aromatic turmeric, 6 parts of radix glycyrrhizae.

The property of medicine and pharmacology of Chinese herbal medicine selected by the present invention is respectively：

3.4.1 radix scutellariae：Hardship, tremble with fear；Return lung, courage, spleen, large intestine, small intestinl channel；Heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis are antiabortive.

Pharmacological action

A, antibacterial action

The concentration of radix scutellariae decoction 100%, flat band method experiment, to shigella dysenteriae, typhoid bacillus, paratyphosum Bacterium, comma bacillus, Escherichia coli, proteus, Pseudomonas aeruginosa, staphylococcus, hemolytic vessel used to hold grain at the imperial sacrifice coccus (a, B), Diplococcus pneumopniae, corynebacterium diphtheriae etc. have Inhibitory action.Radix scutellariae decoction test tube method experiment 1：1280 concentration can suppress typhoid bacillus, hemolytic streptococcus a, and 1：640 concentration press down Hemolytic streptococcus B processed, Diplococcus pneumopniae and shigella flexneri, bacillus tuberculosis typus humanus H37,1：320 concentration can suppress cholera arc Bacterium, Shigella shigae；1：Sonne bacillus has inhibitory action during 80 concentration.Radix scutellariae alcohol immersion liquid 0.5g/ml or 0.05g/ml, with agar slant culture, decoction and culture medium 1：1 mixing, has inhibitory action to Pseudomonas aeruginosa.Radix scutellariae alcohol immersion liquid 2g/ml concentration, Plating experiment have inhibitory action to Escherichia coli, tangerine grass bacillus, staphylococcus aureus.Radix scutellariae decoction, alcohol Agent 1g/ml concentration is put forward, there is suppression effect when being tested with flat band method to meningococcus.

B, antifungic action

Radix scutellariae decocting liquid, test tube slant method test 4% concentration and suppress dog denticle born of the same parents bacterium and trichophyton, and 8% concentration suppresses Oidium schoenleinii, 10% concentration suppress the mutation of oidium schoenleinii Mongolia, and 15% concentration suppresses homocentricity trichophyta and iron rust Color trichophyta.Radix scutellariae flooding agent 1：3 concentration are in vitro to trichophyton, Trichophyton concentricum, oidium schoenleinii, Ao Du Ang Shi microsporums, wool sample microsporum, epidermophyton rubrum, K, W, family name Epidermophyton, star nocardia etc. have Different degrees of bacteriostasis.

C, antivirus action

Radix scutellariae decoction 25-100% concentration, in vitro test are replicated with inhibitory action to hepatitis B virus DNA.

D, anti-inflammatory antiallergic action

The ethanol extract 200 of radix scutellariae 70%, 500mg/kg gavages baicalein, baicalin, wogonin 50,100mg/kg are filled Stomach, mouse peritoneal caused by acetic acid can be suppressed and ooze out increase, to big caused by 48/80 (a kind of compound name, Sigma productions) Mouse is swollen enough also inhibitory action, the ethanol extract 500mg/kg gavages of radix scutellariae 70%, baicalein, baicalin and wogonin 100mg/kg gavages have inhibitory action to rat assist agent arthritis.Root of large-flowered skullcap water extract 100,200mg/kg gavages, to rat quilt Dynamic cutaneous anaphylaxis (PCA) has inhibitory action, but contacts dermatitis (ear swelling) to mouse caused by chloropicrin and have no significant effect. The active ingredient that radix scutellariae suppresses passive cutaneous anaphylaxis (PCA) is baicalin and baicalein.Baicalin, baicalein are to experimental Asthma is effective, and baicalin 10-4g/ml concentration can suppress guinea pig trachea Schultz-Dale reactions 38%, and have antihistamine, resist Choline and the effect of papaverine sample.Baicalein is not soluble in water, two soluble derivative baicalein -6- disodium hydrogen phosphates (BPS) And baicalein -6- disodium sulfates (BSS) 5mg/kg before antigen attack is injected intravenously, and has inhibitory action to P of Rats CA； 10mg/kg is injected intravenously 10 minutes before antigen attack and is administered, and has inhibitory action to cavy allergic asthma；BPS and BSS in 10-4g/ml concentration has inhibitory action to isolated guinea pig intestinal tube and tracheae chultz-Dale reactions；BPS and BSS 5mg/kg Intravenous injection, has inhibitory action to rat Reversed cutaneous anaphylaxis (RCA)；5-10mg/kg intravenous injections also suppress Forssman cutaneous vasculitis, but Arthus reactions are had no significant effect.On baicalein and BPS structures with antiallergic Cromoglycic acid Disodium (DSCG) is similar, but DSCG is double-colored ketone, and baicalein and BPS are monochromatic ketone, and DSCG is to reagin (Reagin) Antibody-mediated allergic reaction has a high degree of specificity, and to several no effects of the antibody-mediated allergic reaction of non-reagin (IgG), PBS then not only suppresses the allergic reaction of reaginic antibody mediation, the monkey as caused by PBS 10-6-10-4g/ml suppress antigen Lung medium discharges, and PBS 200mg/kg intraperitoneal injections suppress allotransplantation in rats PCA, 10-4g/ml and suppress reaginic antibody mediation Rat hypertrophy cell degranulation etc., also suppress the reaction of non-reaginic antibody mediation, as BPS10-4g/ml can suppress white of an egg egg People's lung release Anaphylactic mediator caused by white sensitized guinea pig lung dissolution medium and suppression anti-IgE antibodies, it is believed that two in DSCG molecules Chromone is internuclear certain distance, can be combined in reaginic antibody functional location, and is not suitable for non-reaginic antibody, but monochromatic The baicalein or BPS of ketone can two molecules be incorporated on both antibody.Baicalein is to rat platelet arachidonic acid generation Thanking cyclooxygenase and lipoxygenase has inhibitory action, more selective to lipoxygenase, and its IC50 is 0.12 μm, and to epoxy IC50 is 6917 times of lipoxygenase.Baicalein can suppress arachidonic acid metabolic to rat leukocyte, suppress lipoxygenase metabolism production The life of thing 5- hydroxyeicosatetraenoic acids (5-HETE) and epoxidase metabolite 12- hydroxy heptadecatrienoic acids (HHT) Into its IC50 is respectively 7.13 ± 0.767 μm and 5.53 ± 16.9 μm；Baicalin is formed to leucocyte 5-HETE and also suppressed, but HHT formation is not suppressed；Wogonin suppresses HHT formation, and IC50 is 14.6 ± 3.51 μm of ingredients of Scutellaria to compound 48/80 Stimulation in rats peritoneal cavity mast cells discharge the inhibitory action of histamine, and the IC50 of baicalein is 52.1 μm, and baicalin IC50 is ＞ 200 μm, the IC50 of wogonin is 40.0 μm, and the IC50 of wogonin -7-O-D glucuronic acids is 140.0mol/l, (25) The IC50 of the hydroxyl flavanones of 2 ＇, 5,6 ＇, 7- tetra- is 17.7 μm, (2R, 3R) -2 ＇, 3,5,6 ＇, the IC50 of 7- pentahydroxy- flavanones 15.5 μm, 200 μm of the IC50 ＞ of Chrysin (Chrysin), the IC50 of neobaicalein II (Skullcapflavon II) is 15.0 μ m.Baicalin 10,100mg/ml, it is equal to the rat peritoneal macrophages generation PGE2 and TXA2 of calcium ion carrier A 23187 induction There is obvious inhibiting effect.

E, the effect of Central nervous system

Radix scutellariae decoction 4g/kg is injected intraperitoneally, and to mouse defensive conditionedreflex positive Reflection delay can grow, and to non-bar Part reflects and broken up without influence, illustrates that radix scutellariae can strengthen cortex process of inhibition.Radix scutellariae decoction 2g/kg, typhoid fever polyvalent vaccine is caused Hot rabbit has refrigeration function.But 5-9g/ gavage of radix scutellariae water decoction or Liquor infusion is also had been reported that, or 2g/ im, it can not demonstrate,prove Bright radix scutellariae has refrigeration function to antityphoid vaccine pyrogenicity rabbit.

F, to cardiovascular effect

Radix scutellariae alcohol extract 1g/kg is injected intravenously, and can decline anaesthetized dog blood pressure.Radix scutellariae decoction 0.06g/kg is injected intravenously, There is obvious antihypertensive effect to anesthetized dog.Baicalein 20mg/kg is injected intravenously, and anaesthetized dog blood pressure can be made to decline 40-50%.Radix scutellariae Preserved material gavage/kg gavages, 3 times/day, continuous 4 weeks, make chronic renal hypertension dog drop in blood pressure, heart rate is slack-off.Baicalin 2 × 10 (- 4) mol/l, in the competitive antagonism adrenal gland in guinea pig in vitro aortic article, pulmonary artery bar, tracheal strip and atrium dextrum Contraction caused by element, norepinephrine and dopamine, also antagonism isoprel diastole tracheae and increase atrium dextrum Spontaneous frequency acts on, and prompts baicalin to have blocking effect to a, B1, B2 acceptors.

G, platelet aggregation-against and anti-freezing

Baicalein, wogonin, qroxylin A, skullcapflavone II and Chrysin (chrysin) are when concentration 1.0mM, Collagen-induced rat platelet aggregation can be suppressed, Chrysin also has inhibitory action, baicalein to the ADP platelet aggregations induced And wogonin also has inhibitory action to the platelet aggregation of arachidonic acid-induction, baicalein and baicalin are to thrombin induction Fibrinogen be converted into fibrin and also suppress；Baicalein and baicalin 20,50mg/kg gavages, induced by endotoxin induction Rat disseminated intravascular coagulation, the reduction of blood platelet and fibrinogen content can be prevented.

H, effect for reducing blood fat

10%2ml/ gavage of radix scutellariae infusion, it successive administration 7 weeks, can contain the rabbit anteserum cholesterol that cholesterol is fed with Amount declines.Baicalein, baicalin 100mg/kg gavages, Experimental Hyperlipemia rat corn oil-cholesterol-cholic acid can be reduced and fed The content of serum free fatty acid, triglycerides and liver triglycerides is raised, the 100mg/kg gavages of skullcapflavone II, serum can be reduced The content of T-CHOL and liver triglycerides, the content of increase Serum High-Density Lipoprotein-Cholesterol (HDL-ch), Chinese radix scutellariae Plain 100mg/kg gavages, the deposition of liver triglycerides can be prevented and increase serum hdl-ch content.Baicalein, baicalin 100mg/kg gavages, to hyperlipemia rat caused by ethanol, liver T-CHOL, free cholesterol and triglycerides can be reduced and contained Amount, wogonin can reduce the level of serum triglyceride, and baicalein can increase serum hdl-ch contents.I, liver protection, cholagogic, It is anti-oxidant

Radix scutellariae methanolic extract 1000mg/kg is injected intraperitoneally, to rat hepatic lesion caused by α-naphthyl isothiocyanate (ANIT) There is inhibitory action, the increase of serum bilirubin can be suppressed.Radix scutellariae alcohol extract 50,100mg/kg, baicalin 50,100mg/kg are filled Stomach, there is choleretic effect to rabbit.Wogonin 10 (- 4) -10 (- 6) mol/l concentration in vitro tests, to rat liver microsomes Lipid peroxidation has inhibitory action, declines MDA (MDA) content.Baicalein and baicalin 2.5 × 10 (- 4) mol/l, 1.0 × 10 (- 4) mol/l, wogonin 2.5 × 10 (- 4) mol/l, skullcapflavone II 2.5 × 10 (- 4) mol/l, the Chinese are yellow A kind of reed mentioned in ancient books element -7-O-D glucuronic acids 2.5 × 10 (- 4) mol/l, 1.0 × 10 (- 4) mol/l, to FeC12- vitamin Cs - The rat liver homogenate lipid peroxidation of ADP mixtures induction has inhibitory action, is remarkably decreased liver MDA formation, to NADPH mono- Lipid peroxidation also suppresses caused by ADP.

J, antitumaous effect

Radix scutellariae ether extract has CDCC, effective dose 50 10.4mg/ml, Huang to mouse leukemia L1210 cells A kind of reed mentioned in ancient books flavones II is 1.5 μ g/ml to the effective dose 50 of mouse L1210 cells, and baicalin, baicalein and wogonin are to L1210 Effect is not notable.

K, other effects

Baicalein 10,20mg/kg intravenous injections, has diuresis to anesthetized dog.Radix scutellariae decoction 4g/kg gavages, to rat Galactose cataract has preventive and therapeutic effect, can delay the formation of cataract.Baicalin has suppression to Rat Lens aldose reductase Effect, its ID50 is 1.81 × 10 (- 3) mg/ml.Baicalin 150mg/kg gavages, to diabetes rat caused by broneomycin Blood sugar level is without being decreased obviously, but Red Blood Cell Sorbitol Content significantly reduces after treatment, prompts also have suppression in animal body The effect of aldose enzyme, it is possible to the preventing and treating for diabetic complication.Baicalin, baicalein and wogonin 50-125 μ g/ Ml has inhibitory action to Mouse Liver sialidase.Baicalin 100mg/kg, glucuronic acid 43mg/kg are subcutaneously injected and can resisted Dead mouse caused by strychnine, and glucoside member baicalein is invalid, it is believed that the removing toxic substances that the glucuronic acid after baicalin hydrolysis rises is made With.Metabolism of the radix scutellariae to PGs has wide influence, and biosynthesis of the water extract to PGs has inhibitory action.

3.4.2 the root of herbaceous peony：It is cool in nature, bitter acid, it is slightly cold；With blood-supplementing blood-nourishing, suppressing liver-YANG, the analgesic of soft liver, astringing YIN to stop sweating etc. Effect；Anxious, diarrhea dysentery abdominal pain, spontaneous sweating, uterine bleeding, band suitable for fever due to yin deficiency, irregular menstruation, chest and abdomen pain over the hypochondriac region, four limbs contraction Inferior disease.

Pharmacological action

1st, to the effect of cardiovascular system.Coronary artery dilator, reducing blood pressure, (d- cachou extracts and progallin A have Antithrombotic and antiplatelet aggregative activity)

2nd, liver protection effect.There is obvious protection to make to hepatic injury caused by carbon tetrachloride, aflatoxin B1, D-galactosamine With

3rd, spasmolysis.There is inhibitory action to intestinal tube and stomach in place motion, significantly resist uterine contractile caused by oxytocins

4th, analgesic activity.Mouse writhing can be suppressed, shouted, hot plate reaction, suppressing writhing response to morphine has synergy, And convulsions caused by pentylenetetrazole can be resisted.

3.4.3 fritillaria thunbergii：Hardship, tremble with fear；Return lung, the heart channel of Hang-Shaoyin；Facilitaing lung heat-clearing, preventing phlegm from forming and stopping coughing, open strongly fragrant dissipating bind.Cure mainly wind-heat or phlegm heat Cough, abscess of throat, lung carbuncle, goiter and tumor, sore and toxic.

Pharmacological action

1. antibechic：Peimine and peiminine 4mg/kg gavages, drawing cough to mouse ammonia has inhibitory action, 4mg/ Kg hypodermic injections draw cough to electro photoluminescence anesthetized cat superior laryngeal nerve also antitussive effect.

2. spasmolysis：Fritillaria alkaloid has atropine-like effect, shows that low concentration can make branch to rabbit, cat isolated lung perfusion Tracheae relaxes, and high concentration then has slight shrinkage effect to bronchus.

3. the effect of Central nervous：Peimine and peiminine 2mg/kg are subcutaneously injected, and make the spontaneous work of mouse Dynamic to reduce, 4mg/kg gavages extend mouse sleep time caused by mouse yellow Jackets, and 2mg/kg is subcutaneously injected, to mouse Acetic acid writhing test experiment shows there is analgesic activity.

4. other effects：The various pharmacological actions of peimine and peiminine are quite similar.1：5000-1：1000 is dense Frog heart perfusion is spent, decreased heart rate can be made and produce chamber completely block；10mg/kg is injected intravenously to anesthetized cat, there is antihypertensive effect. 5mg/kg injects to intravenous rabbit, isocratic blood glucose rise in presentation；During 10mg/kg, it is injected intravenously to anesthetized cat, has decompression to make With.5mg/kg injects to intravenous rabbit, isocratic blood glucose rise in presentation；During 10mg/kg, then weakness of limbs, incoordination is presented And tremble；Both minimum lethal doses of mouse mainline are 9mg/kg.Peminoside has stronger drop than peimine Pressure is acted on, and coronary blood flow can be increased by opening born of the same parents dog arteria coroaria sinistra injection 2mg

3.4.4 radix bupleuri：Hardship, it is cool；Enter liver, gallbladder channel；It is soothing the liver in inducing diaphoresis, rising Yang.Control fevers and chills alternate, fullness in the chest and hypochondriac pain, bitter taste Deafness, headache and dizziness, malaria, lower sharp prolapse of the anus, irregular menstruation, metroptosis.

Pharmacological action

(1) it is antipyretic, active ingredient：Volatile oil (eugenol, acid, γ-undecanoic acid lactone and to methoxybenzene diketone), bavin Hu saponin(e (sapogenin A).Effect：Have to heating caused by typhoid fever, paratyphoid vaccine, Escherichia coli liquid, fermented milk, yeast etc. bright Explicit solution heat effect；It and can decline animal normal body temperature.Saikosaponin decoction 2g/kg gives rabbit gavage, to being drawn with vaccine and temperature thorn The heating risen has obvious refrigeration function.

(2) anti-inflammatory, active ingredient：Saponin(e.Effect：And desmoplastic swollen to ankle-joint caused by a variety of proinflammatory agents is scorching Disease has inhibitory action.Saikosaponin saikoside 300mg/kg is injected intraperitoneally, and can suppress carrageenan, serotonin, big caused by histamine Mouse pedal swelling, suppress swollen hyperplasia of rat granuloma, while adrenal gland can be made loose, atrophy of thymus gland；Suppress inflammatory tissue histamine to release Put and leukoplania.

(3) immunologic function, active ingredient are promoted：Bupleurum chinense polysaccharide.Effect：Phagocytic function enhancing, Natural killer activity increase By force, special viral antibody titre is improved, lymphocyte is improved and turns core rate, improve skin delayed hypersensitivity reaction.

(4) anti-liver injury：Bupleurum injection (concentration 1: 1) 1ml/, which is only subcutaneously injected continuous 5 days, can significantly reduce carbon tetrachloride Caused rat blood serum GPT rises, degeneration of liver cells and necrosis also substantially mitigate, glycogen and rna content in liver cell Close to normally.[8]

(5) Antiradiation injury：Bupleurum chinense polysaccharide 5mg/ is only injected intraperitoneally, and can improve the survival rate of irradiation mouse, and mouse thymus is thin 3H-TdR incorporation increase in born of the same parents, while accelerate the release of thymocyte, while make skin in blood plasma again.The increase of matter ketone content, cuts After adrenal gland, there is no these performances, therefore it is considered what is realized by adrenal cortex.In addition, there is anti-tubercle bacillus work in vitro With.Advance on Pharmacological Activities：It is connected with herbal pharmacology research data base.

3.4.5 moutan bark：It is bitter, pungent, it is slightly cold；The thoughts of returning home, liver and kidney channel；Clearing heat and cooling blood, it is promoting blood circulation and removing blood stasis.

Pharmacological action

1st, on cardiovascular influence：Moutan bark can increase the anesthetized dog heart coronary blood flow, reduce cardiac output, reduce left The effect of room work done.There is obvious protective function to expeirmental myocardial ischemia, and the duration is longer, while reduce myocardial oxygen consumption Amount.Root bark of tree peony decoction, the decoction 1.0-3.0g/kg gone after paeonol or paeonol 80-120mg/kg intravenous injections, to anesthetized dog There is antihypertensive effect with rat.Primary and kidney-type hypertension dog, with moutan bark decoction 5g/kg gavages, continuous 5 days, in the 6th day, Dosage increases to 10g/kg within 7th day, and blood pressure is decreased obviously.Kidney-type hypertension dog, the decoction 10g/kg gavages after paeonol are spent, even Continue 10 days drop in blood pressure.Also there is certain antihypertensive effect to kidney-type hypertension dog and rat with paeonol 0.5-1.0g/kg. Paeonol can significantly inhibit the fast phase of normal myocardial cells (5 minutes) and slow phase (120 minutes) 45Ca intakes and Beating Rate, significantly Suppress calcium abnormality cardiac muscle cell 45Ca intakes and reduce intracellular LPO, and be in dose dependent, show paeonol Mitigate Calcium paradox injury with prevent Ca2+ in flow and it is anti-oxidant relevant.Tree peony natrium phenolsulfonicum except can suppress calcium ion intake in addition to, and 45Ca intakes and its after birth SA contents of calcium abnormality cardiac muscle cell can be significantly inhibited, is in dependency relation with dosage.Separately use bait Atherosclerosis Model, study the study of anti-atherogenic effect of paeonol.As a result moulding adds paeonol group aortic tunica intima The deciding grade and level of lesion naked eyes and morphology histochemical analysis, mitigation more notable than moulding control group.Show that paeonol 100mg/ is injected intraperitoneally Kgd, it can substantially suppress atherosclerotic plaque within continuous 6 weeks and be formed.

2nd, the influence of Central nervous system

Paeonol has refrigeration function to mouse heating caused by oral typhoid fever, paratyphoid vaccine, and reduces normal mouse body Temperature.Oral Paeonol can suppress that acetic acid induced mice writhing response and the reaction of rat-tail tenderness is injected intraperitoneally, and can resist caffeine The motion of induced mice is hyperfunction, can be obviously prolonged the ciclobarbital soluble induced mice length of one's sleep, can turn over mouse when heavy dose of Normal reflection disappears, and can substantially resist the convulsions caused by pentylenetetrazol, strychnine, nicotine and electroconvulsive shock.Site of action is netted in midbrain Structure and thalamus.

3rd, antiinflammatory action

With Paeonol gavage, there is suppression to make to rat vola pedis edema caused by dextran or acetic acid or carrageenan With, and mouse peritoneal caused by acetic acid or serotonin or the enhancing of guinea pig skin capillary permeability can be suppressed, suppress mouse The generation of stress ulcer.Experiment proves root bark of tree peony water decoction Carrageenan edema, adjuvant-induced arthritis and Arthus reactions Various inflammatory reactions are inhibited Deng caused by, this biosynthesis for suppressing the permeability of inflammatory tissue with it and suppressing PGE2 Relevant, the root bark of tree peony can not suppress the adrenal compensatory hypertrophy of remaining, and metabolism ascorbic to adrenal gland also has no significant effect, carried Show its effect both without class cortisone sample, also acted on without class corticotropin sample, i.e., its antiinflammatory action is independent of vertical Body adrenal system.Ith, II, type Ⅲ allergy is the reaction mediated by specific antibody, and formation of the root bark of tree peony to antibody has no bright Development rings, but has inhibitory action to it, it may be possible to is played a role by non-specific anti-inflammatory mechanisms, and suppresses serum complement work Property, also just strengthen its anti-inflammatory effect.The root bark of tree peony does not suppress the generation of specific antibody, and the haemolysis for not influenceing alternative pathway of complement is lived Property, moutan bark is prompted while antiinflammatory action is played, it is impossible to suppresses normal body fluid immunologic function.

4th, bacteriostasis

Experiment in vitro shows that moutan bark decoction is to hay bacillus, Escherichia coli, typhoid bacillus, paratyphosum Bacterium, deformed rod Bacterium, Pseudomonas aeruginosa, staphylococcus, hemolytic streptococcus, pneumococcus, comma bacillus etc. have stronger antibacterial action, tree peony Leaf decoction has notable antibacterial action to shigella dysenteriae, Pseudomonas aeruginosa and staphylococcus aureus, and its effective ingredient is gallic acid. Chicken embryo experiment shows that moutan bark decoction infected by influenza has inhibitory action, but mouse Experiment on therapy result is inconsistent, therefore its is disease-resistant Toxic effect fruit can not still be affirmed.Someone is carried out to the antibacterial composition of its water decoction of moutan bark difference decocting time to heat endurance relation Inquire into.Prompting moutan bark In Vitro Bacteriostasis ability after 30 minutes decoct is substantially better than the water decoction that the decoction time is 15 minutes, and With decocting time significant difference is had no for the water decoction bacteriostasis of 60,90 minutes.

5th, anticoagulation

Human blood platelets is tested in vitro, it is found that moutan bark water extract and Chinese herbaceous peony phenol can suppress the production of blood platelet arachidonic acid Raw thromboxane A2, and then suppress platelet aggregation, this is due to suppress to react from arachidonic acid to prostate H2 Cycloxygenase Result.Moutan bark methanolic extract plays the role of experimental thrombosis caused by suppressing endotoxin.Research shows, moutan bark antithrombotic The mechanism of formation is：Paeonol, benzoylpaeoniflorin and the suppression platelet aggregation of benzoyl oxidized paeoniflorin glycosides, and Paeonol, Chinese herbaceous peony Medicine glucoside, oxidized paeoniflorin glycosides have antitropin effect；Benzoylpaeoniflorin has the former activation of barrier fibers plasmin and anti-fiber egg The effect of white lysozyme；Oxidized paeoniflorin glycosides, benzoyl oxidized paeoniflorin glycosides, benzoylpaeoniflorin have stronger stabilization to red after birth Effect, so as to inhibition thrombosis.ADP or collagen-induced blood can be suppressed to rats by intraperitoneal injection or in vitro with Paeoniflorin Platelet is assembled.

6th, to the influence of immune system

Distinguish gavage moutan bark to mouse, Paeonol, Paeoniflorin, oxidized paeoniflorin glycosides, benzoylpaeoniflorin, can promote quiet The carbon granules of arteries and veins injection cleans up speed in blood, and being in low state even if mononuclear phagocyte system function also has promotion to make See that macrophage phagocytic activity strengthens in Kupffer cell and spleen in liver with, microexamination.Paeoniflorin, oxidized paeoniflorin glycosides are in vitro also Phagocytic function of the Turnover of Mouse Peritoneal Macrophages to emulsion can be strengthened.Root bark of tree peony liquid can make spot after its spleen haemolysis to mouse peritoneal injection Number increase.It is used in conjunction 6 days per big 25mg/kg to mouse peritoneal injection with Paeonol, the obvious increase of spleen weight can be made, and can resist and examine Pine, caused by cyclophosphamide thymic weight mitigation.Therefore moutan bark has humidification to body fluid and cellular immunity.

7th, to the influence of lipid-metabolism

The root bark of tree peony and its contained Paeonol, Paeoniflorin have inhibitory action to the lipolysis of the lipocyte caused by adrenaline； Root bark of tree peony water extract can increase glucose generation fat in lipocyte, and substantially increase the glucose generation fat caused by insulin Fat.

8th, other effects

Abdominal cavity is poured into 20% root bark of tree peony Sargent gloryvine, has notable preventive effect to the damaging abdominal cavity adhesion of rabbit.Inject Fu Shi assistants Chornic arthritis mouse caused by agent, in rat skin lower injection proinflammatory agent casein in two months, can cause arthritis foot, The denaturation of tail continues to deteriorate, and foot, the sub-dermal fibrotic of tail, bone regeneration, bone fibres disease is obvious, also there is connective in subcutaneous tissue of abdomen Hyperblastosis, if being continuously fed with moutan bark or guizhi fuling pill while zymoprotein is given, the new of zymoprotein can be suppressed and lured Hair infringement.There is inhibitory action to Mouse Ehrlich Carcinoma Cells, cervical cancer cell in root bark of tree peony methanolic extract body.Paeonol There is certain inhibitory action to metabolism of the BaP in rat liver microsomes, have Robust speaker feature effect to mouse, have diuresis to rat Effect.

3.4.6 stir-baked FRUCTUS GARDENIAE：Hardship, tremble with fear；The thoughts of returning home, lung, tri-jiao channel；Purging intense heat relieving restlessness, reducing fever and causing diuresis, removing pattogenic heat from the blood and toxic material from the body.

Pharmacological action

1st, choleretic effect

The composition such as cape jasmine and contained iridoid glycoside has choleretic effect.Its alcohol extracting thing and safflower glucoside, crocetin Choleresis can be increased.Its water extract and alcohol extracting thing are oral to rabbit, to Amount of Bile derived from biliary pore and solid Composition is injected in rabbit without influence, but with same preparation, and choleresis starts to increase within 15~30 minutes, continues more than 1 hour.Give After intravenous rabbit injects crocin and crocetin sodium, choleresis increase.To cape jasmine main component geniposide cholagogic machine The research of system shows：When geniposide is administered in alimentary canal, Geniposide is generated because of hydrolysis.Geniposide is administered in portal vein Shi Bucheng choleretic effects, the choleretic effect of this explanation geniposide is come into force by the Geniposide generated.Geniposide is aobvious It when writing increase bile flow, can decline bile acid concentration in bile, but on the excretion of bile acid substantially without influence.

Cape jasmine water decoction or granule or powder preparation to be taken after being infused in boiling water make gall-bladder film making after giving people orally prove there there are substantially 20 and 40 minutes gall-bladders after medication Contraction.Cape jasmine can reduce the content of serum bilirubin：After the ligation of rabbit total biliary pore, oral cape jasmine aqueous extract then blood Mesobilirubin is reduced, and the more, reduction is more notable for medication, and alcohol extract also has identical effect, but slightly weak compared with aqueous extract effect. Cape jasmine alcohol extract is injected in rabbit, and 2 hours blood mesobilirubin slightly increases compared with control group, low compared with control group after 6 hours, and 24~48 Significantly reduced after hour, crocin and crocetin are received, and also have same purpose.In the rabbit of common bile duct ligation, alcohol extracting is injected Take liquid, tip lymph mesobilirubin is reduced within 24 hours, and crocin and crocetin, which are received, also same purpose.Cape jasmine can be used for Jaundice caused by biliary tract inflammation, but removing jaundice mechanism is more complicated.

Research shows：Capejasmine extract is free of toxic effects to liver cell.Cape jasmine can reduce serum bilirubin level, but and Portugal Grape uronic acid transferase is unrelated.Cape jasmine can also mitigate hepatic lesion caused by carbon tetrachloride.

2nd, to the effect of intestines and stomach and pancreas

Geniposide produces the inhibitory action of anticholinergic to stomach function.Geniposide duodenal administration, to pylorus ligation Rat suppresses in gastric secretion, and total acidity gastric juice reduces, and gastric juice pH rises.The same dose intravenous administration of Geniposide, to rat Hyperfunction motion caused by the motion of body stomach transient can suppress its autogenic movement and pilocarpinum, and can subtract stomach tension force Small, for intestine in vitro, Geniposide is in weak competitive antagonism to the contraction caused by acetylcholine and pilocarpinum.

Cape jasmine can promote pancreatic secretion.Geniposide significantly reduces amylopsin, and its enzymolysis product capital Buddhist nun Flat increase pancreas courage flow effect is most strong, and the duration is shorter.In pancreatitis, cape jasmine is improved body resistance against diseases, improves liver The pharmacological action such as the dirty and function of gastronintestinal system and mitigation pancreatitis.

3rd, the effect of Central nervous system

Cape jasmine alcohol extracting thing can reduce spontaneous activity in mice, have sedation, and work is cooperateed with ciclobarbital soluble With can extend the length of one's sleep, can still resist the convulsions of pentylenetetrazol.Though the convulsions of strychnine can not be resisted, it is dead that it can be reduced Rate.Have been reported that after mouse peritoneal injection cape jasmine alcohol extracting thing 1 hour, body temperature averagely reduces by 3 DEG C, rats by intraperitoneal injection cape jasmine 200mg/kg, sustainable more than the 7 hours of temperature decline it is believed that cape jasmine is calm, active ingredient of cooling effect is ursolic acid, It can improve the mouse half seizure dose caused by pentylenetetrazol, there is obvious anticonvulsant action.Experiment shows：Ursolic acid has obvious Central inhibitory action can substantially reduce the normal body temperature of big white mouse；There is certain synergy to hyoscine；It can reduce small Mouse spontaneous activity and the sleep of collaboration sub-threshold dose yellow Jackets；It can substantially resist and be fainted from fear caused by pentylenetetrazol；But it there are no Obvious analgesic activity.This experimental result is prompted, and ursolic acid has similarity with chlorpromazine.Also it is reported that, cape jasmine water Extract, geniposide can suppress the acetic acid twisting reaction of mouse, therefore think there is analgesic activity.

4th, resisting pathogenic microbes acts on

Cape jasmine has inhibitory action to staphylococcus aureus, diplococcus meningitidis, micrococcus catarrhalis etc., and agent, which has, kills hook end spiral shell The effect of body and imago of blood fluke is revolved, infusion has inhibitory action to a variety of dermatophytes in vitro.In addition, cape jasmine ethanol extracts Thing, water extract, ethyl acetate portion and geniposide play the role of certain anti-inflammatory and treatment soft tissue injury.Its extract Ointment is made, the healing of soft tissue can be accelerated.

5th, to the effect of cardiovascular system

Capejasmine extract can reduce myocardial contractive power.Anesthetized dog, mouse intravenous capejasmine extract, can be because of heart retraction volume and the heart Output quantity declines and causes drop in blood pressure.During rat intravenous heavy dose cape jasmine methanolic extract, myocardial damage can be presented in electrocardiogram And atrioventricular block.

The hypotensive activity position of cape jasmine is in maincenter, caused by mainly strengthening medulla oblongata parasympathetic maincenter tensity.When cutting After disconnected both sides vagus nerve, the hypotensive activity of cape jasmine is obviously reduced or is wholly absent；Atropine can also cancel its antihypertensive effect. Zoopery shows：Cape jasmine decoction and alcohol extracting thing, no matter which kind of approach administration has antihypertensive effect, intravenously administrable is depressured fast Speed, hold time also of short duration, the hypotensive activity of cape jasmine to adrenaline boosting and blocks the pressurization of carotid artery flow anti- No influence is penetrated, also without the effect for strengthening acetylcholine.Due to the hypotensive activity of cape jasmine be not caused by histamine release, also with Afferent neurofibers is unrelated, the uninterrupted effect of neuromere, so giving antihistamine such as diphenhydramine or intravenous procaine all The blood pressure lowering effect of cape jasmine can not be changed.

6th, other are acted on

Cape jasmine has certain anastalsis, and the anastalsis of cape jasmine is strong compared with stir-baked FRUCTUS GARDENIAE.Geniposide has to mouse rush down under Effect.

3.4.7 Poria cocos：It is sweet, light, flat, enter the heart, lung, the spleen channel；Eliminating dampness and diuresis, strengthening the spleen and stomach, the effect of antitoxic heart-soothing and sedative.

Pharmacological action

1st, diuresis

Diuresis：0.5g/Kg is injected intraperitoneally to normal rabbit in 25% Poria cocos alcohol preserved material, diuresis occurs.With excision kidney The rat experiment of upper gland proves that absorption of the diuresis with influenceing renal tubule Na+ is relevant.

There is report to point out, after Poria cocos water medicinal extract and alcohol immersion liquid are to gavages such as rabbit, rat, mouse, hardly show profit Urine acts on, when ureterostoma dog chronic experiment is injected intravenously decoction, also without diuresis.But also there is report to think, with clear-headed Rabbit chronic experiment proves that Poria cocos truly has diuresis, and being compareed with ash content proves, its diuresis is not due to sylvite, but sylvite with The effect of outer other compositions.The hot water extract of Poria cocos and water-soluble polysaccharide component be not notable to normal rabbits effect, if Fu With ginger, radix glycyrrhizae, then 1 hour urine volume steadily increases Siberian cocklebur compatibility after dispensing, thereafter effect lasts.It is clinical by Poria cocos and the bighead atractylodes rhizome, life The compatibilities such as ginger are used for the symptoms such as chronic nephritis, and urine volume has considerable degree of increase after offeing medicine 1 hour.Prompting is in Li Shui effect sides Face, compound can produce continuous action than simple.

The principle of Poria cocos diuresis not yet illustrates.Someone is compareed with Poria cocos ash content with 25% Poria cocos alcohol immersion liquid, knot Fruit the latter shows diuretic effect, and the former is then without effect, illustrates that Poria cocos contains special diuresis composition.It is big with adrenalectomize White mouse is tested, and injection desoxycortone merges using 30% Poria cocos decoction than alone desoxycortone person hydrouria, urine sodium, urine Potassium exclusion amount also increases, it is thus regarded that Poria cocos acts on without anti-desoxycortone, and with influenceing weight of the renal tubule to sodium ion Absorb relevant.

2nd, sedation

Poria cocos decoction mouse peritoneal injection, its spontaneous activity can be substantially reduced, and it is excessive to resist caffeine induced mice It is excited；Anesthetic effect to yellow Jackets has obvious synergy.Poria cocos can strengthen pentothal to mouse CNS inhibition Effect, anesthesia duration significantly extend.

Decoction particularly fushen's parenteral solution, has sedation to animal.

3rd, to the effect of cardiovascular system

In the perfusion experiment of marmot, toad and Rana esculenta isolated heart, the water extract of Poria cocos, ethanol extract, Ether extract can strengthen myocardial contractive power, increased heart rate.

Poria cocos water medicinal extract and ethanol extract have hypoglycemic effect to rabbit.Poria cocos water, ethanol, ether extract are in vitro The frog heart has enhancing to shrink, accelerates heart rate function.

4th, to the effect of digestive system

Poria cocos has significant protective effect to the mouse liver injury caused by carbon tetrachloride, and gpt activity can be made bright It is aobvious to reduce, prevent necrosis of liver cells.Poria cocos preserved material has direct relexation to family's rabbits infestines, drops smooth muscle contraction amplitude Low, tension force declines, and has inhibitory action to ulcer caused by rat pylorus ligation.And gastric secretion and free acid content can be reduced.

5th, antitumor, antitumaous effect

Pachyman is in itself without antitumor action.Pachymaran (i.e. pachymaran), carboxymethylpachymaran are to mouse Euphorbia egg decoctum solid type and ascites turn solid type, cervix cancer S14 solid types and ascites turn solid type etc. have it is different degrees of Tumor-inhibiting action.More than pachymaran intraperitoneal administration 10mg/kg, can suppress mouse S180 implanted solid tumor growths, but with antineoplastic 5- The use in conjunction such as fluorouracil, endoxan have no obvious synergy, to external S180 cells without direct killing effect.Carboxylic first Base pachymaran has stronger inhibitory action to mouse transplanting tumor U14；Also there is certain inhibitory action to ehrlich ascites cancer.Experiment Show, DNA synthesis of the carboxymethylpachymaran to ehrlich ascites cell has inhibitory action.Su-fuling is to murine interleukin The DNA synthesis of L1210 cells has obvious and irreversible inhibitory action, and inhibitory action is strengthened with the increase of dosage.Poria cocos Element has synergistic effect to anticarcinogen, and tumor suppression (S180 sarcoma) rate shared with mitomycin is that 48% (mitomycin is alone For 35%)；It is 38.9% (alone dactinomycin D is 19.6%) with the tumour inhibiting rate that dactinomycin D shares；Suppression is shared with endoxan Ratio of outflow is 69.0% (alone endoxan is 32.3%)；It is 59.1% (5- fluorine ureas with the tumour inhibiting rate that 5-fluor-uracil shares 38.6%) alone pyrimidine is.To mouse leukemia L1210, the increase in life span that endoxan is used alone is 70%, su-fuling Shared with endoxan as 168.1%.

On Poria cocos antineoplastic action mechanism, have it is demonstrated experimentally that carboxymethylpachymaran antitumor action has with thymus gland Close.Also there is report to point out, pachymaran activation local complement, make the complement that tumour close region is activated thin by influenceing macrophage Born of the same parents, lymphocyte or other cells and humoral factor, so as to Synergistic killing tumour cell.Carboxymethylpachymaran is to Emhorn ascites The inhibitory action of cancer cell is realized by suppressing DNA synthesis.

Antitumaous effect：Pachymaran has inhibitory action to S180 sarcoma, and inhibiting rate is up to 96.88%.From artificial depth In the Poria mycelium that layer culture obtains, pachymaran F1 and H11 are can extract, has obvious antitumor activity.

6th, other are acted on

Poria cocos decoction takes orally, can make red rose pigment rate and phytohemagglutin phytolectin induce lymphocyte transformation rate it is notable on Rise.Pachymaran has anti-atrophy of thymus gland and the function of the increase of anti-spleen and tumor suppression growth, and pachymaran gavage can strengthen mouse Macrophage phagocytic function, increase ANAE positive lymphocyte numbers, moreover it is possible to make mouse spleen antibody secreting cell number showed increased. Pachymaran can also make the big white mouse Neuroleptic Leukocytopenia caused by endoxan accelerate to go up.Carboxymethylpachymaran also has immune Regulation, protecting liver, lowering enzymes, indirectly it is antiviral, induce with Stimulation effect interferon, mitigate radiation side reaction, induce and Stimulation effect leucocyte A variety of physiologically actives such as element are adjusted, without bad toxic side effect.

Experiment in vitro shows that Poria cocos decoction has inhibitory action to staphylococcus aureus, Escherichia coli, proteus.Second Alcohol extracting thing can kill Leptospira, and water decoction is then invalid.

Immunological enhancement：Pachyman has humidification to normal and tumor-bearing mice immunologic function, can strengthen mouse Macrophage phagocytic function.

3.4.8 the bighead atractylodes rhizome：It is bitter, sweet, warm；Returns spleen, stomach；It is strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect, hidroschesis, antiabortive.

Pharmacological action

1st, diuresis

With obvious and lasting diuresis, there is effect to various animals such as rat, rabbit, dog.It is quiet to not anaesthetizing dog Arteries and veins injects decoction 0.05-0.25g/kg, and urine volume increase is remained above after 5 hours in medication normal up to more than 9 times；Gavage is given Medicine 1-3g/kg, urine volume compared with can increase 2-6 times before medication, and majority after medication 6-7 hours still more than normal, the bighead atractylodes rhizome is decocted Agent and liquid extract 1.0g/kg can be produced substantially and persistently to rat intravenous injection, rabbit 1.0g/kg gavages or intraperitoneal injection Diuresis.The bighead atractylodes rhizome not only increases the excretion of water, also promotes the discharge of electrolyte particularly sodium, and the excretion of sodium is also better than water Excretion.It nor affects on the antidiuretic activity of pitutrin, therefore the excretion of bighead atractylodes rhizome increase water may be primarily not shadow The initiative reabsorption of Xiangshui County, but supervention is in the re-absorbed reduction of electrolyte, the effect of the existing excretion of mercusal sample chlorine, sodium； Have again increase urine in carbon dioxide capacity, pH value and increase potassium excretion, reduce ammonium excretion acetazolamide sample the characteristics of.To people Diuresis have a small number of experiments, it is impossible to finally affirm.

2nd, hypoglycemic effect

Rabbit gavage decoction or medicinal extract, blood glucose are in a slight decrease.Rat oral gavage decoction have the assimilation of glucose in acceleration bodies because And reduce blood glucose.Mouse, which takes decoction orally, protection liver, prevents liver glycogen reduction caused by carbon tetrachloride from acting on.

3rd, strong effect

The bighead atractylodes rhizome decoction gavage lmol or 6g/kg, mouse weight can be promoted to increase and strengthen swimming endurance, the bighead atractylodes rhizome can strengthen net The phagocytic function of shape endothelial system, it is in activation to mouse reticuloendothelial system, promotes the phagocytosis of Turnover of Mouse Peritoneal Macrophages Function, makes the phagocytic percentage of macrophage, and phagocytic index and its lysosomal digestion averagely dramatically increase compared with control group.White thin When born of the same parents reduce disease, the bighead atractylodes rhizome has leukogenic effect.The bighead atractylodes rhizome can also improve lymphocyte transformation rate and natural rose formation rate, promote thin Born of the same parents' immunologic function, and substantially increase IgG.Illustrate that the bighead atractylodes rhizome plays the role of strengthening the spleen and stomach, strong body and improves resistance against diseases.

4th, blood coagulation resisting function

The bighead atractylodes rhizome has obvious inhibitory action to platelet aggregation, bighead atractylodes rhizome decoction 0.5g/kg gavages l-4 weeks, can significantly extend The time of rat prothrombing.Its effect is weak compared with bicoumarin, but is strong compared with Butadion.The effect of root is stronger than stem.Healthy People Take 5% decoction, every time 1 soupspoon, 3 times a day, after 4 days prothrombin time and clotting time significantly extend, after drug withdrawal The level that 10 days These parameters are returned to before administration, alcohol leachate is also effective, but it is shorter to hold time.

5th, to the effect of cardiovascular system

The bighead atractylodes rhizome has vasorelaxation action.It is in inhibitory action to heart, can causes to stop winning when dosage is excessive, anesthetized dog intravenous injection Decoction 0.1g/kg, when blood pressure slightly declines 0.25g/kg, blood pressure drastically declines, and recovery is had no in 3-4 hours.

6th, antitumor action

In vitro test shows that the neutral oil in Baizhu volatile oil has obvious inhibiting effect to esophageal cancer cell.10mcg/ml When, in 24 hours cancer cell being made all to come off.During 5mcg/ml, most of cancer cell can be made to come off, remaining small pieces cell Or disseminated cell is in karyopycnosis, kernel is smudgy, the more vacuoles of alveolar substance.Baizhu volatile oil 50-100mg/kg intraperitoneal injections are to Chinese mugwort Family name's ascites carcinoma significantly inhibits effect.Then reported during Formulations for systemic administration, the effect of to substantive solid carcinoma inconsistent.358 kinds are planted When thing medicine, cmm of simple prescription and compound are screened, inhibitory action most strong (inhibiting rate of the Baizhu volatile oil to murine sarcoma -180 For 31-49%) separately have been reported that to S-180, S-37, U-14 and W-256, and (L615 makees Leukemia Model without obvious With.Nearly report, the bighead atractylodes rhizome significantly increase the tardy of MethA tumours to such as activity increase more obvious than control group in methA tumours Property hypersensitivity, if also promoting PHA-P and lipopolysaccharide-induced children to change reaction.

7th, to the effect of gastrointestinal smooth muscle

Past reports, the bighead atractylodes rhizome to gastrointestinal function (gastric secretion of such as hydrochloric acid in gastric juice, pushability enterocinesia etc.) without influence, also without Antiulcer, anti-inflammatory and analgesic activity, do not influence normal body temperature, and Central nervous system is considered as without obvious inhibiting effect, the latter Can be as the foundation distinguished with rhizoma atractylodis.Nearly report, influence of the bighead atractylodes rhizome to isolated small intestines of rabbits spontaneous activity differ more, in vain Art can strengthen the activity of Isolated rabbit intestine Spontaneous Contraction, increase its shrinkage amplitude, and Rhizoma Atractylodis Macrocephalae oil suppresses the autogenic movement of intestinal tube, or Autogenic movement of the bighead atractylodes rhizome on isolated small intestines of rabbits influences unobvious.Rhizoma Atractylodis Macrocephalae extract 50mg and 200mg/kg gastric infusion, to dynamic Thing water-immersion restraint stress ulcer, significantly inhibits effect.

8th, antibacterial action

Infusion in vitro has inhibitory action to acrothesium floccosum, star nocardia.Decoction is to meningococcus Also there is inhibitory action.Nearly report bighead atractylodes rhizome decoction and Sijunzi Tang to typhoid bacillus, paratyphoid bacillus A, shigella flexneri, Escherichia coli, Pseudomonas aeruginosa etc. have different degrees of bacteriostasis, and without bactericidal action.

9th, hematopoiesis function is promoted

The bighead atractylodes rhizome decoction 1g/kg, 0.2ml/, which are only subcutaneously injected, can promote mouse bone marrow cells hemopoietic progenitor cell of red blood cell line (CFU-E) Growth.Decline for the white blood cell caused by chemotherapy or radiotherapy, having makes its elevated effect.

10th, protein synthesis is promoted：

Bighead atractylodes rhizome decoction 10g/kg gavages, continuous 7 days, hence it is evident that promote the synthesis of mouse small intestine protein.

11st, other effects

The bighead atractylodes rhizome has of short duration excitation to breathing, in addition uterine smooth muscle of the bighead atractylodes rhizome to rabbit, intestines mouse, rat and mouse There is obvious inhibiting effect, bighead atractylodes rhizome decoction has protective effect to mouse hepatic injury caused by Carbon tetrachloride.Bighead atractylodes rhizome ethyl acetate carries Thing is taken, big white mouse duodenal administration, can substantially increase choleresis.A small amount of volatile oil has sedation.

3.4.9 the bark of official magnolia：It is bitter, pungent, warm；Returns spleen, stomach, large intestine；Gas consumption product, eliminating dampness except full, relieving asthma.

Pharmacological action

1、Antibacterial action

The second and methanolic extract of the bark of official magnolia have strong antibacterial to make to cariogenic bacteria streptococcus mutans (Streptococusmatuans) With its Antibacterial Constituents is defined as Magnolol and Honokiol.Both are at the minimum inhibitory concentration (MIC) to streptococcus mutans 6.3 μ g/ml, its antibacterial action are more stronger than berberine (to streptococcus mutans).The first deduction of the bark of official magnolia divides magnocurarine to be volatilized with the bark of official magnolia Oily saturated aqueous solution has certain bacteriostasis to staphylococcus aureus, sarcine and hay bacillus.The bark of official magnolia is double to pneumonia Coccus and shigella dysenteriae also have antibacterial activity.

The bark of official magnolia has certain protective effect to the hepatic lesion caused by zoopery venereal disease virus hepatitis, and right Epsteinbarr viruses have inhibitory action.

2nd, alimentary canal is acted on

Magnocurarine gradually rises in vitro rabbit duodenum tension force in the range of 0.1-1ml (10mg/ml), to 1.5ml When, Muscle tensility gradually suppresses, frequency deceleration, stops completely to myenteron contraction movement during 2.5ml.The anti-rat of bark of official magnolia alcohol extract Hcl- ethanol ulcer function rat body weight 200g, the oral ethanol extract of the bark of official magnolia 50% of administration group, while control group is set, l is administered After hour, the methanol of Hcl- ethanol 15% is given, l puts to death animal after hour.Ulcer length (mm) aggregate value occurred with membranous part As ulcer index, compared with control group.As a result administration group is in one of notable inhibitory action, its active component to gastric mucosa ulcer It is defined as honokiol and magnolol.The irritability acute gastric ulcer of rat is caused using water logging stimulus method, with perfusion method in stomach Determine rat gastric secretion, determine under simulated gastric fluid perfusion conditions stress load rat gastrorrhagia, the results showed that magnolol Have the function that to prevent irritability stomach dysfunction.

3rd, the inhibitory action of platelet aggregation

Magnolol and Honokiol suppresses the aggregation of the rabbit platelet rich plasma of collagen and arachidonic acid-induction and ATP is released Put, aggregation of the washed platelet aggregation than platelet rich plasma becomes apparent from being suppressed.The aggregation of whole blood is less to be pressed down by the two Preparation influences.Magnolol and Honokiol suppresses thromboxane B2 and formed in all cases, is caused by arachidonic acid or collagen Intracellular Ca rise also suppressed by both.The antiplatelet effects of Magnolol and Honokiol are due to thromboxane B2 and thin The suppression of intracellular Ca flowings.

4th, Central nervous systemic effect

Magnocurarine has relexation to striated muscle.Intravenous injection makes rabbit dosage of hanging one's head be 13.8mg/kg, with same dosage repeatedly The bark of official magnolia

Rabbit intravenous is awarded, its Muscle relaxation does not weaken, and prompts no tachylaxis.In nervus phrenicus-diaphragm of rat In the experiment of flesh sample, 30% magnocurarine can make contraction of diaphragm amplitude reduce 40% or so, and when concentration increases to 40%, diaphram is received Contracting degree almost halted state.When chicken is injected intravenously more than magnocurarine 40mg/kg, in flaccid paralysis, it is acted on and vein It is similar to inject tubocurarine (0.5mg/ is only), shows that magnocurarine may belong to the muscle relaxant of nondepolarizing type.Contained by the product Magnolol and iso-agnolol there are central myorelaxant effects.During larger dose, righting for mouse can be made counter to thank to disappearance. Chicken spinal cord is counter to thank in experiment, and magnolol and iso-agnolol intraperitoneal injection can substantially suppress extensor reflex, and this effect can be big Dosage strychnine institute antagonism, it is believed that they belong to non-curariform muscle relaxant and its effect than U.S.A is new (Mephenesin) power By force, show that magnolol and iso-agnolol have special and lasting muscle relaxant activities.Magnolol passes through veutro with frog During spinal artery, the anti-current potential of thanking of root declines slowly before only making, and dorsal root potential is almost without influence.Magnolol passes with myelosuppressive The interaction led between material GABA and the precursor glutamic acid of excitability transmitter shows that magnolol can significantly suppress paddy The effect of propylhomoserin, and to GABA without effect.Show that magnolol plays its effect in spinal cord by the interaction with mediator, And specificity is very high, there is unique mechanism of action.Mouse is injected intraperitoneally respectively and oral bark of official magnolia ether extract, passes through metal Net pendency method easily detects grip and reduced very much；Play the role of to suppress spinal reflex by the detection of chicken intercrossing extension reflex, show There is central Muscle relaxation.Bark of official magnolia ether extract is for by the drug-induced spasm such as strychnine, picrotoxin, pentylenetetrazole There is strong inhibitory action.Bark of official magnolia ether extract shows the slow waveization effect for comparing mitigation to spontaneous brain electricity ripple, particularly exists See that spindle occurs in cortical electrograms.In the case where stimulating reticular formation of brain stem or thalamus in anterior waking response moderate stimulation threshold value Rise, show there is inhibitory action to brain stem reticular activating system and hypothalamus activation system.Magnolol and Honokiol is also to ridge Spinal reflex has strong inhibitory action.Rabbit is hung one's head experimental rabbit 3, and the iodomethane chemical combination of bark of official magnolia alkaloid is slowly injected from ear vein The normal saline solution (40mg/ml) of thing, generation rabbit, which hangs one's head to react, to stop, dosage power of averagely hanging one's head 330mg/kg.Inject to 1/3 Occur a large amount of salivations during dosage, intravenous injection neostigmine methyl sulfate parenteral solution can resist the reaction, with the raw rattan of muscle relaxant tin Alkali pharmacological action has in place of approximation.

5th, hypotensive activity

Cat (body weight 2.1-2.7kg) l only, is injected intravenously magnocurarine 3mg/kg, drop in blood pressure about 20mmHg, lasts about 10 Minute.

6th, bornyl magnolol has anti-allergy action

7th, bark of official magnolia methanolic extract honokiol has significantly to mouse skin tumour caused by internal the second stage of carcinogenic test Inhibitory action.

3.4.10 dried orange peel：It is warm, pungent, bitter；Enter the spleen channel, stomach, lung channel；Regulating QI and lowering the adverseness, whet the appetite in adjusting, be eliminating dampness and eliminating phlegm.

3.4.11 semen armeniacae amarae：It is hardship, tepor, slightly poisonous；Return lung, large intestine channel；Sending down abnormally ascending is relieving cough and asthma, relaxes bowel.

Pharmacological action

1st, antibechic, antiasthmatic effect

Contain amarogentin in semen armeniacae amarae, amarogentin in vivo can be contained in itself by enteric microorganism enzyme or semen armeniacae amarae Emulsin hydrolyzes, and produces micro hydrogen cyanide and benzaldehyde, has inhibitory action to respiratory center, reaches antibechic, antiasthmatic effect. 《Book on Chinese herbal medicine is looked for the truth》Record " almond, the energy of existing expelling cold, eliminating, there is the power that lower gas removes asthma again ".It is apt to relieving cough and reducing sputum and relievings asthma, to lung The chief complaint cough of system, abundant expectoration, pant and have strong specific aim, therefore doctor uses as the medication of lung system at all times, nothing By internal injury diseases caused by external factors, new sick chronic illness is all to relate to lungs, uses it.Tested using guinea pig in vitro organ, almond water extracting solution can reduce device The sensitiveness that official irritates to ammoniacal liquor, to the excitation of antihistamine, acetylcholine, barium chloride to tracheal smooth muscle, have obvious Antitussive action.Cause cough method to prove using SO2, kept to mouse stomach amarogentin 1mg/kg, 10mg/kg, 100mg/kg After 30min, its inhibiting rate to frequency of coughing is respectively 26%, 22.8%, 25.3%.Gavage Semen Armeniacae Amarum extract 48.3mg/ Kg effect is stronger than equivalent amarogentin by 39.7%.Semen armeniacae amarae promoting pulmonary surfactant (PS) synthesis because being advantageous to Pulmonary respiratory function, semen armeniacae amarae can not only promote PS synthesis, and can improve the various physiology about lung and biochemical indicator (such as Total phospholipids and pathological section etc. in lung homogenate, edema with the lung involved amount, bronchial lavage).

2nd, to the effect of digestive system

Gas under almond bitter, and it is rich in fat oil.Fat oil can improve lubrication of the intestinal contents to mucous membrane, therefore almond There is the function of relaxing bowel.Amarogentin also produces benzaldehyde while hydrogen cyanide is decomposed to form through enzyme effect, and the latter can press down The activity of pepsin processed, so as to influence digestive function.The pepsin hydrolyzate of almond water-soluble portion is with 500mg/kg The rat administration that handles carbon tetrachloride of dosage, it is found that it can press down that AST, ALT be horizontal and the rise of hydroxyproline content, and press down The extension of euglobulin lysis time processed.The pepsin hydrolyzate of almond water-soluble portion can suppress mouse liver connective tissue Hyperplasia, but the horizontal rise of mouse AST, ALT caused by D-galactosamine can not be suppressed.

3rd, anti-inflammatory, analgesic activity

The amygdalate water extract of degreasing is scorching to the mouse writhing reaction caused by acetic acid and the rat granuloma caused by cotton balls The formation of disease has inhibitory action.With rat podarthrum edema method, the water soluble protein composition of intravenous injection 40mg/kg almonds There is suppression inflammatory effect when KR-A, KR-B5mg/kg.Shown with mouse naphthoquinones induced pain model test, KR-A or KR-B veins When 5mg/kg is administered, suppression pain rate is respectively 40.7% and 58.2%.They are swollen enough to Rat Carrageenan colloidality, oral uptake ED50 is respectively 13.9mg/kg and 6.4mg/kg.Hot plate method in mice and acetic acid writhing method confirm that amarogentin has analgesic activity, But different from the analgestic of morphine.The quiet styrax condensation enzyme effect generation styrax of benzaldehyde caused by amarogentin decomposition.Peace Breath perfume has analgesic activity, therefore domestic someone can release the pain of patient with semen armeniacae amarae treatment advanced liver cancer, and some is not even Anodyne need to be taken.

4th, antitumor action

Early in the twentieth century amarogentin is separated from almond first, and amarogentin is used for by nineteen twenty first in the U.S. Treat tumour.Ernest doctors Krebs are first people that amarogentin is used for medicine in the U.S., and it is referred to as tieing up and given birth to Plain B17.Though amarogentin is used for treating tumour, business in the national widespread such as the U.S., Mexico since the 1950s The name of an article is laetrile or laetrile, but the antitumor action about amarogentin does not obtain recognizing for FDA, in science There is dispute on boundary always, two kinds of views completely contradicted be present.A favorable side thinks that amarogentin is a kind of laetrile, Have the function that to prevent and treat tumour, and propose some theoretical and test datas.Such as think that tumour cell anaerobic degradation is dominant Gesture, final product are lactic acid, and slant acidity environmental benefits promote amarogentin in tumour in the activity for improving beta-glucosidase More benzaldehyde and hydrogen cyanide are decomposited in cell, and produces the selective killing effect to tumour cell.In contrast to this Viewpoint but thinks that amarogentin is not vitamin, without antitumaous effect, or even noxious material yet.Relevant semen armeniacae amarae Though the antitumor action of glycosides is still being argued, in 20 including Belgium, Germany, Italy, Mexico and Philippine It is multiple country in, manufacture and the use of amarogentin is legal.

Antitumor curative effect about almond, there are a large amount of reports both at home and abroad, but result is not the same.Help Teng Zhaoyan (1979 Year) report almond hot water extract thick agent be 50%-70% to the inhibiting rate of Human cervical tumor's JTC-26 strains.Fang Wenlong Result of study Deng (1986) shows that Semen Armeniacae Amarum extract intraperitoneal injection or gavage, the inhibiting rate to liver cancer and sarcoma 180 are 61.7%-77.1%.First-class (1987) result of the test of car text shows, the antitumor active ingredient extracted from semen armeniacae amarae to Transplanted hepatoma mouse peritoneal injection 300mg/kg, 400mg/kg, 600mg/kg, its tumor control rate is respectively 72.0%, 60.8%th, 51.0%.Semen armeniacae amarae is freely absorbed to mouse, the growth of ehrlich ascites cell can be suppressed, and makes life span extension. Also have been reported that amarogentin can prevent and treat the liver cancer of N-nitrosodimethylamine induction, reduce tumour stove.But wheat Wei Qi etc. (1981 Year) amarogentin (10% aqueous solution) progress animal experiment is extracted from semen armeniacae amarae, the results showed that it is equal to rat liver cancer, S180 etc. Without bacteriostatic activity.By NCI (American Cancer Society) initiate relevant amarogentin the human cases of cancer curative effect are observed in Terminate within 1978 but do not draw obvious conclusion.The U.S. has carried out a variety of frontier nature clinical tests in nineteen eighty-two, it is believed that Laetrile does not have the clinical value for the treatment of cancer.

Research about almond antitumor machanism mainly has following several respects.

1. a part of scholar thinks to contain a large amount of beta-glucosidases in cancer cell, the enzyme can hydrolyze amarogentin generation Hydrogen cyanide (HCN), benzaldehyde and glucose.Because cancer cell lacks sulphur nitrile generation enzyme, the enzyme has the detoxication to HCN, HCN is set to become nontoxic sulphur nitrile compound, and normal cell lacks beta-glucosidase and generates enzyme containing substantial amounts of sulphur nitrile.According to According to the theory, amarogentin should be able to selectively kill cancer cell, and normal cell is almost harmless.

2. amarogentin can help the transparent sample mucin film of internal Trypsin Induced cancer cell, make internal leucocyte more Accessible cancer cell, and swallow cancer cell.

3. it has been found that amarogentin can influence thymidine similar to NaSCN and NaOCN enters liver tumor cell DNA With absorption of the tumour cell to phosphate and amino acid.

5th, hypoglycemic effect

Amarogentin has the function that to prevent and treat the diabetes caused by antineoplastic alloxan.The high blood of mouse is induced using alloxan Sugared method proves, surveys blood glucose after 3g/kg amarogentins 48h is injected intraperitoneally in advance, the results showed that amarogentin can specifically suppress Blood glucose rise caused by alloxan, action intensity are relevant with the concentration of amarogentin in blood.

6th, effect for reducing blood fat

Clinical report, 24 blood fat are 200~260 patient, daily to raw almond 100g as primary fat source, 3 weeks Afterwards, blood fat averagely declines 10.1%.85 hyperlipemic patients take 75g jordan almonds daily, continuously take 4 weeks, are cutting out After be further continued for observation 4 weeks.As a result show, jordan almond can substantially reduce the blood lipid level of hyperlipemic patients, wherein total with serum The decline of cholesterol (TC) and LDL-C (LDL-C) is the most obvious, declines 10.6% and 8.1% respectively, together When patient HDL-C (HDL-C) there is no significant change, apolipoprotein B (Apo B) is decreased obviously, carry fat Albumin A 1 (Apo A1) substantially rises.Spiller (nineteen ninety) thinks that the monounsaturated fatty acids in almond helps to reduce and suffered from Person's slightly elevated blood fat, and without strict dietary restriction.

7th, beautification function

It is theoretical according to lung associating with skin and hair, clinically some disease of skin can be controlled from facilitaing lung method opinion, compatibility eats almond, often Obtain prompt effect.Modern study proves that contained fat oil can soften keratoderma in semen armeniacae amarae, moisturize skin care, there is patron saint Effect through peripheral vessel and histoorgan, and bactericidal can be pressed down.In addition, the HCN generated by enzyme hydrolysis can suppress internal Active tyrosine enzyme, pigmentation, freckle, blackspot etc. are eliminated, so as to reach the effect of beauty.

8th, other are acted on

The researchs such as Zhao Sulian are found, NK cells in mice activity and thorn can be obviously promoted to mouse intramuscular injection amarogentin Swash the proliferation of T lymphocytes.Li Chunhua etc. observes amarogentin in same experimental animal and swallows work(to Gan Kang Yin Can influence, and observe with Ag-NOR methods the relation with Gan Kang Yin rDNA activations.As a result show, amarogentin [3mg/ (d)] has obviously facilitation to its phagocytic function and to Gan Kang Yin rDNA activation, has Strengthen the function of immunity of organism.Amarogentin has antimutagenic effect, can reduce and be drawn by analgin, metronidazole, mitomycin C etc. The quantity of the micronucleus polychromatic erythrocyte risen.

Almond oil also has expelling parasite, bactericidal action, and in vitro test has killing effect to Ascaris lumbricoides, earthworm, and to typhoid fever, pair Typhoid bacillus has antibacterial action.Almond also has anti-pinworm and trichomonas infection, treatment alpastic anemia effect.Amarogentin The effect of obvious is also show in rat liver fibrosis caused by treating rabbit blood fluke characteristic of disease liver fibrosis and carbon tetrachloride, its The mechanism of action and increase hepatic blood flow, improve degrading activity of the clostridiopetidase A to I, III, IV property collagen, and by influenceing IV property glue The formation that the former metabolism with flaggy element disturbs capillarization of sinuscids is relevant.

3.4.12 root tuber of aromatic turmeric：It is arduous, it is cool；Enter the heart, lung, Liver Channel；Promoting qi circulation and removing obstruction in the collateral, cool blood broken stasis of blood.The all pains of chest and abdomen side of body rib are controlled, lose the heart It is demented, pyreticosis coma, spit blood, bleeding from five sense organs or subcutaneous tissue, hematuria, blood strangury, women's vicarious menstruation, jaundice.

Pharmacological action

1st, to the effect of blood fat

Root tuber of aromatic turmeric water decoction is given because cholesterine causes the rabbit of atherosclerosis oral 10g/ days, and serum courage is consolidated after 100 days Alcohol does not drop, is anti-height compared with control group, phosphatide rise is more apparent, and beta lipoprotein, triglyceride slightly rise, but and control group Indifference turmeric powders are given the big white mouse of experimental atherosclerosis by 134mg/100g, and serum cholesterol and C/P values have slightly Rise, but the formation of rabbit or rat aorta and coronary endometrium patch can be mitigated and lipidosis but also have report to refer to Go out, congener Curcuma amada ether extract gives the rabbit with hypercholesterolemia oral, and blood courage was made in three weeks Sterol is down to 36mg% by 266mg%, and C/P values are also corresponding to be declined, and sustainer weight substantially mitigates, the weight of animals increase.

2nd, antifungic action

Flooding agent (1：3), in vitro to trichophyton, Trichophyton concentricum, perhaps trichophyton gypseum, blue favus The dermatophytes such as bacterium, microsporum audouini, microsporum ferrugineum have different degrees of inhibitory action.

3rd, medicine (poison) is of science

With body weight 21-25g female mices 50, it is divided into 5 groups, every group 10, by the rhizoma curcumae longae concisa of intraperitoneal injection various dose Water decoction.It is administered once, observes 72 hours, records dead animal number.It is 33.79g/kg to try to achieve LD50 by karber's method, and SD is 3.09g/kg.After administration 5 minutes, mouse activity is reduced, and heavy dose of group, in all death in 0.5 hour, is had a convulsion before dead.It is low Dosage group mouse upon administration, slightly in rest state, recovers normal on the 2nd day.Curcumin capsule Mouse oral 6g/kg is surveyed not Go out median lethal dose.Rat subacute toxicity test result shows, to rat appetite, body weight, general status, hematology and blood Biochemical index and liver, kidney weight have no significant effect.

3.4.13 radix glycyrrhizae：It is sweet, flat；The heart, lung, spleen, stomach；Invigorate the spleen and benefit qi, be clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain, tune With all medicines.

Pharmacological action

Western modern medicine haircut is existing, and radix glycyrrhizae agent has a function of anti-inflammatory and resistance state reflection, thus doctor trained in Western medicine clinically mainly as Moderator.Alleviate cough, eliminating the phlegm, treat pharyngalgia laryngitis；Radix glycyrrhizae or enoxolone have the effect of desoxycortone class, in chronic renal Gland cortical hypofunction has good effects；Glycyrrhiza preparation can promote stomach mucus to be formed and secreted, and extend the epithelial cell life-span, There is anti-inflammatory activity, be usually used in the treatment of chronic ulcer and duodenal ulcer；The flavones of radix glycyrrhizae has anti-inflammatory, spasmolysis and antiacid work With；Radix glycyrrhizae is also one of primary raw material of jintan.

Radix glycyrrhizae is also widely used for food industry, refines candy, preserved fruit and chewing gum.Extract of licorice root is manufacture chocolate Emulsifying agent, moreover it is possible to increase the vinosity and fragrance of beer, improve the denseness and color and luster of dark beer, make some soft drinks and sweet tea Wine；Cigarette flavoring.In chemical industry, printing and dyeing industry, radix glycyrrhizae also extensively has purposes.

1st, to the effect of digestive system

Flavonoid glycosides has obvious protective function to Rat Experimental ulcer in the medicinal extract of removing glycyrrhizin and radix glycyrrhizae.

2nd, adrenocortical hormones fuction

Extract of licorice root, glycyrrhizin and enoxolone have promotion sodium, the effect of water retention to Healthy People and animal；Small dose Amount glycyrrhizin (every 100ug) can make rat chest gland atrophy and the increase of adrenal gland weight, produce glucocorticoid cortisone sample Effect.Then glucocorticoid sample effect unobvious, a presentation mineralocorticoid sample act on when heavy dose of.

3rd, detoxication

Extract of licorice root and glycyrrhizin have certain to some drugses poisoning, food poisoning, interior metabolism product poisoning Detoxification ability.The effective ingredient of detoxication is glycyrrhizin, and detoxication mechanisms are that glycyrrhizin has suction-operated, radix glycyrrhizae to poisonous substance Glucin hydrolysate glucuronic acid can be combined with poisonous substance, and glycyrrhizin is known as adrenocortical hormones fuction, strengthens liver The result of dirty detoxification ability etc. combined factors effect.

4th, function of relieving cough and calming asthma

Enoxolone has obvious central antitussive effect, and heavy dose of enoxolone can suppress mouse breathing.In addition Glycyrrhizin, glycyrrhetate still have anti-inflammatory and antiallergy, anti-liver injury, anti-promoting, antibacterial, anti AIDS virus (glycyrrhizin Element) effect.

5th, body's immunity, anti-arrhythmia are adjusted

The technical problems to be solved by the invention are to provide a kind of, few side effects better than existing curative effect of medication, can answered for a long time For controlling the Chinese medicine composition of the liver cancer patient state of an illness.It using the Chinese herbal medicine of Chinese tradition is raw material that medicine of the present invention, which is, through section Prescription, and the theory verified what is be formulated repeatedly, meet traditional Chinese medicine for passing through clinical test.

Between every taste Chinese medicine in the present invention, by being drawn after multiple clinical prescription compatibility, wherein radix scutellariae is monarch, is act as Heat-clearing and damp-drying drug, purging intense heat and detonicating and other effects；White peony root is used as ministerial, there is the work(such as blood-supplementing blood-nourishing, suppressing liver-YANG, the analgesic of soft liver, astringing YIN to stop sweating Effect；Fritillaria thunbergii is assistant, performs facilitaing lung heat-clearing, preventing phlegm from forming and stopping coughing, opens strongly fragrant dissipating bind and other effects；It is radix bupleuri to make medicine, plays with inducing diaphoresis, dredges The effect of liver rising Yang.The main nature and flavor of this composition are bitter cold, and effect is mainly, clearing heat and detoxicating, qi-supplementing, blood-engendering, and it is thin to suppress tumour Born of the same parents breed, and promote apoptosis of tumor cells, suppress tumor cell adhesion, prevent Nasopharyngeal neoplasms.Its preparation method is simple, can be with The formulations such as piece agent, pill, powder or oral liquid are made as needed, and after taking, medicine is started with from the totality of people, simultaneous to sample The purpose controlled.

In addition, treatment of this composition to primary carcinoma of liver has the effect of fine, few side effects, energy prolonged application, it is not easy Recurrence, and it is without any side effects, do not develop immunity to drugs, after taking half a year, effective percentage reaches 50%.The healing example of 1 year reaches 25%.

Further to investigate the clinical efficacy and safety of medicine of the present invention, we have selected 20 patients to medicine of the present invention The treatment curative effect of thing carries out clinical test.

MethodsThe cases enrolled：In 20 patients through being treated with this prescription, wherein man 15, female 5, the course of disease is most short 2 months, most long April.

Efficacy determination：Using WHO criterions of therapeutical effect

(1) complete incidence graph (CR)：Visual tumors disappear and continue more than January.

(2) (PR) is alleviated in part：The maximum orthogonal diameter product of two, tumour reduces more than 50% and continues one More than month.

(3) it is stable (SD)：The maximum orthogonal diameter product of two, tumour is reduced less than 50%, and increase is no more than 25% and continue more than one month.

(4) (PD) is deteriorated：The maximum orthogonal diameter product increase of two, tumour is no more than 25%.

This formula treats primary carcinoma of liver, and the effective percentage of half a year is 50%；The cure rate of 2 years is 25%.

Laboratory research：

Research shows：Baicalein has tumor-inhibiting action to the tumor-bearing mice of Murine Hepatoma22 cell line.Baicalein can significantly inhibit Model mice tumor growth in vivo, inhibiting rate are respectively 44.65%-61.63%；It is remarkably improved G0/G1 phase cell percentages Than, and reduce S phase cell numbers.The notable inducing cell apoptosis of baicalein, the Capcase-3 activity in tumor tissue cell is activated, Suppress Bcl-2 genes expression, and in induced tumor tissue Bax genes expression.Baicalein can also raise miR-34a table Reach, suppress the propagation of tumour cell, promote the apoptosis of tumour cell.

The propagation of liver cancer cells has obvious relation, the target therapeutic agent Suo Nafeini of liver cancer with raf-1 signal paths Mainly by suppressing raf-1 activity, the signal of MAPK paths, which passes to, to be suppressed.In Huppert's disease, baicalein also can The signal for suppressing MAPK paths passes to, moreover it is possible to suppresses gene expression and the anti-apoptotic of interleukin-6 by NF- B approach Albumin X IAP expression.Therefore, baicalein energy inducing cell apoptosis, causes the growth rate of liver cancer cells to reduce, anti-apoptotic energy Power declines, and reaches the purpose of clinical treatment Huppert's disease.

Clinical controlled trial

This medicine is mainly used in the treatment of primary carcinoma of liver, by the observations of more than 3 years it was found that, with 10 control groups (symptomatic treatment) is compared, this medicine group (10), and patient's extends 3 times without disease developing time (PFS), and most short PFS extends super 4 times are crossed, most long PFS was more than 3 years.Illustrate in the therapeutic process of liver cancer, this medicine group is better than control group.

The effect of 2 medicines of table are used for primary liver cancer is observed

In addition, present invention demonstrates that, this curative effect of medication is good, few side effects, can prolonged application, during medication, patient without turn The generation of shifting, it is uneasy to recur and without any side effects, do not develop immunity to drugs, the effective percentage for taking later six months half a year reaches 50%, the cure rate of 1 year is up to 25%.

There is the effect of fine to primary liver cancer, can effectively extend the paracmasis of patient, reduce the number of recurrence, carry High quality of life.This medicine has no toxic side effect, and efficient high, medical expense is low, and patient is easily accepted by.

It using the Chinese herbal medicine of Chinese tradition is raw material that medicine of the present invention, which is, the prescription through science, and passes through clinical test Checking is formulated repeatedly, meets the theory of traditional Chinese medicine.

Embodiment

Embodiment 1：

Weigh 15 grams of radix scutellariae, 15 parts of the root of herbaceous peony, 20 parts of fritillaria thunbergii, 15 parts of radix bupleuri, 10 grams of moutan bark, 10 grams of stir-baked FRUCTUS GARDENIAE, Poria cocos 10 grams, 10 grams of the bighead atractylodes rhizome, 10 grams of the bark of official magnolia, 6 grams of dried orange peel, 6 grams of semen armeniacae amarae, 20 parts of root tuber of aromatic turmeric, 3 grams of radix glycyrrhizae.

Preparation process is as follows：

Each herbal medicine is weighed by formula rate, being boiled into decoction by traditional handicraft takes or morcel into fine powder respectively, by tradition Pharmaceutical technology is processed into tablet, pill, powder or oral liquid described in pharmacy.

Embodiment 2：

Weigh 20 grams of radix scutellariae, 20 grams of the root of herbaceous peony, 25 grams of fritillaria thunbergii, 20 grams of radix bupleuri, 15 grams of moutan bark, 15 grams of stir-baked FRUCTUS GARDENIAE, Poria cocos 15 grams, 15 grams of the bighead atractylodes rhizome, 15 grams of the bark of official magnolia, 12 grams of dried orange peel, 12 grams of semen armeniacae amarae, 25 grams of root tuber of aromatic turmeric, 6 grams of radix glycyrrhizae.

Preparation process is the same as embodiment 1.

Embodiment 3：

Weigh 30 grams of radix scutellariae, 30 grams of the root of herbaceous peony, 40 grams of fritillaria thunbergii, 30 grams of radix bupleuri, 30 grams of moutan bark, 30 grams of stir-baked FRUCTUS GARDENIAE, Poria cocos 20 grams, 20 grams of the bighead atractylodes rhizome, 20 grams of the bark of official magnolia, 18 grams of dried orange peel, 18 grams of semen armeniacae amarae, 40 grams of root tuber of aromatic turmeric, 12 grams of radix glycyrrhizae.

Preparation process is the same as embodiment 1.

Embodiment 4：

Weigh 35 grams of radix scutellariae, 35 grams of the root of herbaceous peony, 45 grams of fritillaria thunbergii, 35 grams of radix bupleuri, 30 grams of moutan bark, 30 grams of stir-baked FRUCTUS GARDENIAE, Poria cocos 25 grams, 25 grams of the bighead atractylodes rhizome, 25 grams of the bark of official magnolia, 24 grams of dried orange peel, 24 grams of semen armeniacae amarae, 45 grams of root tuber of aromatic turmeric, 12 grams of radix glycyrrhizae.

Preparation process is the same as embodiment 1.

Embodiment 5：

Weigh 40 grams of radix scutellariae, 40 grams of the root of herbaceous peony, 50 grams of fritillaria thunbergii, 40 grams of radix bupleuri, 40 grams of moutan bark, 35 grams of stir-baked FRUCTUS GARDENIAE, Poria cocos 30 grams, 30 grams of the bighead atractylodes rhizome, 30 grams of the bark of official magnolia, 30 grams of dried orange peel, 30 grams of semen armeniacae amarae, 50 grams of root tuber of aromatic turmeric, 15 grams of radix glycyrrhizae.

Preparation process is the same as embodiment 1.

Embodiment 6

Weigh 20 parts of radix scutellariae, 15 parts of the root of herbaceous peony, 30 parts of fritillaria thunbergii, 25 parts of radix bupleuri, 25 parts of moutan bark, 25 parts of stir-baked FRUCTUS GARDENIAE, Poria cocos 20 parts, 20 parts of the bighead atractylodes rhizome, 20 parts of the bark of official magnolia, 18 parts of dried orange peel, 18 parts of semen armeniacae amarae, 30 parts of root tuber of aromatic turmeric, 6 parts of radix glycyrrhizae.

Preparation process is the same as embodiment 1.

Typical clinical case

Case 1：Liu, female, 81 years old, make a definite diagnosis primary carcinoma of liver 3.5 years, previously there is a hepatitis B and hepatitis medical history, 2010 Through the course for the treatment of of Radio Frequency Operation Treatment 2, one course of therapy of chemotherapy is ineffective, 2011, to our hospital, is diagnosed as second for our hospital Liver, hepatitis, hepatic sclerosis, hepatic coma, liver cancer and Bone tumour, check find the enclosed mass of a visible a diameter of 5cm in liver.Controlled routinely On the basis for the treatment of scheme, start plus with this composition, 3 times a day, after taking January, enclosed mass has no increase in liver；Continuous treatment 2 Nian Hou, enclosed mass disappears in liver, and various Index for examination are stable, including erythrocyte sedimentation rate, C reactive protein, albumin, globulin, sick so far Feelings are stable.

Case 2：Peng, female, 57 years old, make a definite diagnosis liver cancer nearly 4 years, previously have hepatitis B and hepatitis medical history, 2011, come our hospital just Examine, be diagnosed as hepatitis B, hepatitis, hepatic sclerosis, liver cancer, check finds the enclosed mass of a visible a diameter of 4cm in liver, and alpha-fetoprotein is 5000ng/ml.On the basis of Conventional treatment regimens, start plus with this medicine, 3 times a day, after taking January, enclosed mass is opened in liver Begin to reduce；After continuous treatment 1.5 years, enclosed mass disappears in liver, and various Index for examination are stable, including liver function, erythrocyte sedimentation rate, C- reaction Albumen, albumin, globulin, alpha-fetoprotein recover normal for nearly 1 year completely, so far stable disease.

Case 3：Lu, man, 55 years old, makes a definite diagnosis liver cancer 1 year, during onset, visible huge enclosed mass in liver, because lump is close Artery in liver, it can not perform the operation and cut out, and a large amount of ascites occur.January comes our hospital after diagnosis, in check B ultrasound prompting liver it is visible always Footpath is 10cm enclosed mass, is being started to add with this medicine, 3 times a day, after taking 2 months, enclosed mass is contracted to a diameter of 8cm in liver, respectively Kind Index for examination recovers normal completely, and ascites disappears, stable disease.

Case 4：Xiao, female, 35 years old, because making a definite diagnosis primary carcinoma of liver January, after being admitted to hospital.On the basis of conventional scheme treatment, Adding with 2 courses for the treatment of of this medicine, stable disease, multiple enclosed mass is obviously reduced in liver, then using maintaining treatment after 1 year, disease Feelings are stable.

Case 5：Huang, man, 40 years old, peasant, was diagnosed as hepatitis carrier, because hepatalgia is examined before 1 year before 20 years Break as primary carcinoma of liver, local doctor suggests abandoning chemotherapy and operation, using treatment by Chinese herbs.Now suit the medicine to the illness in application and support is controlled On the basis for the treatment of, add with this medicine, by the treatment of 1 year, at present, conditions of patients was stablized always, and quality of life is high.

Claims (3)

1. A kind of 1. Chinese medicine composition for primary carcinoma of liver auxiliary treatment, it is characterised in that：The composition is by following weight The Chinese herb of part is made：Radix scutellariae 20-30 parts, root of herbaceous peony 20-30 parts, fritillaria thunbergii 25-40 parts, radix bupleuri 20-35 parts, moutan bark 20-30 parts, 30 parts of stir-baked FRUCTUS GARDENIAE 20-, Poria cocos 15-25 parts, bighead atractylodes rhizome 15-25 parts, bark of official magnolia 15-25 parts, dried orange peel 12-24 parts, semen armeniacae amarae 12-24 parts, root tuber of aromatic turmeric 25-40 parts, radix glycyrrhizae 6-12 parts.
2. A kind of 2. Chinese medicine composition for primary carcinoma of liver auxiliary treatment, it is characterised in that：The composition is by following weight The Chinese herb of part is made：20 parts of radix scutellariae, 15 parts of the root of herbaceous peony, 30 parts of fritillaria thunbergii, 25 parts of radix bupleuri, 25 parts of moutan bark, stir-baked FRUCTUS GARDENIAE 25 parts, 20 parts of Poria cocos, 20 parts of the bighead atractylodes rhizome, 20 parts of the bark of official magnolia, 18 parts of dried orange peel, 18 parts of semen armeniacae amarae, 30 parts of root tuber of aromatic turmeric, 6 parts of radix glycyrrhizae.
3. 3. the Chinese medicine composition for primary carcinoma of liver auxiliary treatment described in claim 1 or 2 is primary for preparing treatment Application in the preparation of property liver cancer.