CN104396814A - Indoor bostrichthys sinensis fry intermediate rearing method - Google Patents

Indoor bostrichthys sinensis fry intermediate rearing method Download PDF

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Publication number
CN104396814A
CN104396814A CN201410598971.0A CN201410598971A CN104396814A CN 104396814 A CN104396814 A CN 104396814A CN 201410598971 A CN201410598971 A CN 201410598971A CN 104396814 A CN104396814 A CN 104396814A
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seed
plastic box
seawater
rearing
salinity
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CN104396814B (en
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张钰霆
洪万树
毛勇
李哲
陈仕玺
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Xiamen University
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Xiamen University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention relates to an indoor bostrichthys sinensis fry intermediate rearing method, and relates to fish fry rearing. According to the invention, pipes and air pumps are placed in a plastic tank; the plastic tank is filled with seawater; bleaching powder is added for disinfection, and the plastic tank is washed by using seawater; a sun shade net is washed by using seawater, and is exposed under sunlight; living water salinity of the fries before capturing is determined; if the living water salinity is different by no more than 10 than the seawater salinity in the plastic tank, the fries are directly placed in the plastic tank for intermediate rearing; if the salinity difference is higher than 10, the fries are subjected to 1-2 days of transition, and are transferred into the plastic tank for intermediate rearing; the fries are not fed the day the fries are placed into the plastic tank; after that, the fries are fed with bait-feed once every morning and evening; fry rearing water is replaced every two days; light intensity is kept below 6lux and seawater dissolved oxygen is maintained at (5.84+/-0.184)mg L<-1>. When fries achieve full lengths of 8.2cm and weights of 8.0g with intermediate rearing, the fries are transferred into a culturing pond and are subjected to adult fish culturing.

Description

A kind of Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of indoor
Technical field
The present invention relates to fish fry to cultivate, especially relate to a kind of Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of indoor.
Background technology
Bostrichthys sinensis (Bostrichthys Sinensis Lac é pede) is under the jurisdiction of Chordata (Chordata), Vertebrate (Vertebrate), Osteichthyes (Osteichthys), Actinopterygii (Actinopterygii), (Perciformes), Acenthogobius flavimanus suborder (Gobioidei), Eleotridae (Eleotridae), Bostrichthys sinensis belong to (Bostrichthys) to Perciformes.Custom claims Charybdis tiger, snakeheaded fish, painting fish, moon fish, native fish etc., and body back of the body taupe, the outside of belly is light brown, and tail fin base portion has black circular spot.Bostrichthys sinensis is river mouth salt-fresh water water warm predacious fish, is distributed widely in the Indian Ocean northern littoral, and to the east of Pacific Ocean Melanesia and Polynesia, north, to Japan, reaches Indonesia in the south.The North Sea, the South Sea, the Taiwan Straits and the East Sea are originated in China.Bostrichthys sinensis inhabits in cave, intertidal zone, and vitality is strong, and growth is fast, and strong to the tolerance of salinity, can survive in river mouth salt-fresh water, be suitable for pond or mudflat aquaculture, be a kind of excellent cultivation fingerling.Over nearly 30 years, Bostrichthys sinensis cultivation is promoted in coastal area of southeastern China.At present, the seed of Bostrichthys sinensis cultivation is most absolutely by artificial culture (Lin Zhenwei, the healthy Cultivating techniques of Bostrichthys sinensis seed.Chinese Fishery, 2010 (4): 56; Dote on China. Li Mingyun, the biological property of Bostrichthys sinensis and artificial breeding progress.Oceanography Institute Of Zhejiang's journal (natural science edition), 21 (3): 269 ~ 272).In order to improve cultivation survival rate and save aquaculture cost, seed needs an intermediate rearing process before putting in a suitable place to breed.Owing to not grasping the technical method of Bostrichthys sinensis intermediate rearing in the past, do not reach desirable way to cultivate.Therefore, in the urgent need to a kind of Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of indoor.
Summary of the invention
The object of this invention is to provide a kind of Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of indoor.
The present invention includes following steps:
1) cultivate equipment, comprise plastic box, pipe, sunshade net, aerator and gas charging stone; Before using, pipe and gas charging stone are put into plastic box, in plastic box, fill with seawater, then add bleaching powder sterilization, then with clean sea water cleaning, be exposed to the sun after the cleaning of sunshade net seawater;
2) seed rearing process:
(1) measure seed catch before Living Water salinity, if seed catch before Living Water salinity differ with the seawater salinity in plastic box≤10, then direct plastic box of being put into by seed carries out intermediate rearing; If salinity difference > 10, then prepare the seawater of suitable salinity, then allow seed transition proceed to again in plastic box after 1 ~ 2 day to carry out intermediate rearing;
(2) throw in the not feeding on the same day of seed, the bait 1 time of sooner or later respectively throwing something and feeding every day afterwards, checks whether seed has death simultaneously;
(3) 2h after feeding, remove the residual bait in plastic box and excreta, within every 2 days, seed rearing water is changed by needs;
(4), in whole intermediate rearing process, create entirely dark living environment with sunshade net, the light intensity in plastic box is less than 6lux, and the dissolved oxygen of plastic box maritime interior waters remains on (5.84 ± 0.184) mg L -1, seed through intermediate rearing, when total length reach 8.2cm, body weight reach 8.0g time, namely proceed to cultivating pool and carry out adult fish culture.
In step 1) in, described plastic box can adopt the blue plastic box of long 65cm × wide 45cm × high 40cm; Described pipe can adopt long 56.5cm, diameter 10.7cm, thickness to be the cylindrical pvc pipe of 0.2cm; Described sunshade net can adopt the black sunshade net of long 5m, wide 2m; Described seawater can adopt the seawater after extra large sand and the double filtration of cotton yarn bag, and the salinity of seawater can be 15, and the dissolved oxygen content of seawater can be (5.84 ± 0.184) mg L -1; Light intensity in plastic box is less than 6lux, when light intensity is greater than 6lux, can increase the number of plies of sunshade net; The usage amount of described bleaching powder can be 250g; The time of described sterilization can be 24h; The described time of being exposed to the sun can be 1 ~ 2 day.
In step 2) in (1) part, described seed can choose total length 3 ~ 4cm, the Bostrichthys sinensis seed of body weight 0.2 ~ 0.4g.
In step 2) in (2) part, the quantity of described input seed can be in each plastic box puts into 15 tail seeds; The time of described morning and evening can be morning 8 and dusk 6 points; Described bait can adopt Articial bait making.
In step 2) in (3) part, residual bait in described removing plastic box and excremental method can adopt siphon pipe to remove residual bait in plastic box and excreta, sunshade net can not be raised completely when carrying out this operation, extraneous light should be avoided as far as possible to irradiate and enter in plastic box; Described seed rearing water is changed and the seawater in plastic box can be changed 45% ~ 55%; Described seed rearing water is changed and is preferably selected between the lights or evening, avoids seed to be subject to the stimulation of high light; The concrete grammar that described seed rearing water is changed can be: get out the clean seawater consistent with plastic box maritime interior waters salinity, first with siphon pipe by the seawater sucking-off 45% ~ 55% in plastic box, then add the ready clean seawater of equal volume.
In step 2) in (4) part, the time of intermediate rearing can be 50 days.
The present invention is by 4 salinity gradients and 4 lighting gradients experiments, filter out salinity and the illumination condition of the growth of the most applicable Bostrichthys sinensis seed and survival, for the intermediate rearing how realizing Bostrichthys sinensis seed provides technical scheme, to the sustainable development of Bostrichthys sinensis aquaculture industry, there is important using value.
Embodiment
The present invention is further illustrated for following examples.
Prepare 8 long 65cm × wide 45cm × high 40cm, blue plastic box that volume is 140L, the hollow cylindrical pvc pipe of 8 long 56.5cm, diameter 10.7cm, thickness 0.2cm and 8 gas charging stones.Be positioned in plastic box by pvc pipe and gas charging stone, in case, fill with seawater, add the bleaching powder of 250g, stir soaking disinfection 24h.Simultaneously cutting 8 black sunshade nets (long 5m, wide 2m), with seawater clean up be placed on sunlight under be exposed to the sun 1 day.With clean sea water, the blue plastic box through sterilizing, pvc pipe and 8 gas charging stones are cleaned 3 times, until these equipment do not remain bleaching agent.June 27, in each plastic box, place 1 pvc pipe and 1 gas charging stone, in plastic box, then add the clean seawater 100L through extra large sand and the double filtration of cotton yarn bag.8 plastic box distributions arrange the nurturing an environment of different salinity and illumination, for filtering out the condition of the most applicable Bostrichthys sinensis seed intermediate rearing.When ensureing that other are consistent, wherein 4 plastic boxes arrange different salinity gradients, and another 4 plastic boxes arrange different periodicity of illumination gradients.Salinity gradient is respectively 5,15,25 and 35 (normal light cycle); Periodicity of illumination gradient is distributed as 0h, 4h, 8h, 12h (salinity is 15).The preparation of different salinity seawater: for high salinity group (25 and 35), first contain into clean sea water in clean container (200L white plastic case), add appropriate clean sea salt again, measure the proportion of Dinghai water after stirring with densimeter, become the salinity gradient of design by formula scales.For Low-salinity group (5 and 15), first contain into clean sea water in clean container (200L white plastic case), add the appropriate running water after 24h aeration again, after stirring, use densimeter to measure the proportion of Dinghai water, become design salinity by formula scales.By black sunshade net doubling 1 time, cover above plastic box, to prevent light from entering in plastic box, measure the intensity of illumination in plastic box with intensity of illumination analyzer, intensity of illumination in plastic box is controlled be less than or equal to 6lux.Different light application time experiments is regulated by the time length of raising black sunshade net.Carry out blowing aeration with aerator and gas charging stone to the seawater of plastic box, inflation intensity adjustment presents minute bubbles to water surface.Measure through dissolved oxygen meter, the dissolved oxygen content in water body is 5.84mgL-1.
Choose the Bostrichthys sinensis seed (total length 3 ~ 4cm, body weight 0.2 ~ 0.4g) of 120 tail bouncings every other day, be divided into 8 groups at random, often organize 15 tails, throw in 8 respectively and prepared in the plastic box of seawater salinity.Because the life seawater salinity of seed is 20, before being thrown in different salinity gradient group, all will (seed as salinity 35 groups needs first to support 1 day temporarily in the seawater of salinity 25, and the rest may be inferred through the salinity transitional period of 1 ~ 2 day.)。When starting feeding, morning 8 every day and at dusk 6 Articial bait makings of respectively throwing something and feeding (multiplex (MUX) factory is known by day clear fish meal EP1 Marubeni Nisshin Feed Co., Ltd. one) 1 time.In cultivating process, within every 2 days, with the ight soil bottom siphon pipe sucking-off plastic box and residual bait, change the cultivation water of 50% simultaneously.Specifically change water method as follows: get out the clean seawater (method as above-mentioned) consistent with plastic box maritime interior waters salinity, first with siphon pipe by plastic box 50% seawater sucking-off, then add equal volume, the clean seawater of filtration that salinity is consistent.Water temperature during seed rearing and dissolved oxygen content are respectively 29.4 DEG C ± 0.703 and 5.84mg L -1± 0.184.The body that nurturing period measures weekly seed grows (L) and body weight (W), calculates special rate of body weight gain (SGR) and plentiful index (CF), add up survival rate simultaneously with this.
Cultivation result through 50 days shows, the experimental group of full dark situation and seawater salinity are the experimental group of 15, Bostrichthys sinensis seed is special rate of body weight gain the highest (19.58%) weekly, survival rate 95%, average body length and body weight reach 8.2cm and 8.0g respectively, plentiful index 1.27, moves into adult fish culture pond and cultivates.

Claims (10)

1. an indoor Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method, is characterized in that comprising the following steps:
1) cultivate equipment, comprise plastic box, pipe, sunshade net, aerator and gas charging stone; Before using, pipe and gas charging stone are put into plastic box, in plastic box, fill with seawater, then add bleaching powder sterilization, then with clean sea water cleaning, be exposed to the sun after the cleaning of sunshade net seawater;
2) seed rearing process:
(1) measure seed catch before Living Water salinity, if seed catch before Living Water salinity differ with the seawater salinity in plastic box≤10, then direct plastic box of being put into by seed carries out intermediate rearing; If salinity difference > 10, then prepare the seawater of suitable salinity, then allow seed transition proceed to again in plastic box after 1 ~ 2 day to carry out intermediate rearing;
(2) throw in the not feeding on the same day of seed, the bait 1 time of sooner or later respectively throwing something and feeding every day afterwards, checks whether seed has death simultaneously;
(3) 2h after feeding, remove the residual bait in plastic box and excreta, within every 2 days, seed rearing water is changed by needs;
(4), in whole intermediate rearing process, create entirely dark living environment with sunshade net, the light intensity in plastic box is less than 6lux, and the dissolved oxygen of plastic box maritime interior waters remains on (5.84 ± 0.184) mg L -1, seed through intermediate rearing, when total length reach 8.2cm, body weight reach 8.0g time, namely proceed to cultivating pool and carry out adult fish culture.
2. the Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of a kind of indoor as claimed in claim 1, is characterized in that in step 1) in, described plastic box adopts the blue plastic box of long 65cm × wide 45cm × high 40cm; Described pipe can adopt long 56.5cm, diameter 10.7cm, thickness to be the cylindrical pvc pipe of 0.2cm; Described sunshade net can adopt the black sunshade net of long 5m, wide 2m.
3. the Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of a kind of indoor as claimed in claim 1, is characterized in that in step 1) in, described seawater adopts the seawater after extra large sand and the double filtration of cotton yarn bag; The salinity of described seawater can be 15, and the dissolved oxygen content of seawater can be (5.84 ± 0.184) mg L -1.
4. the Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of a kind of indoor as claimed in claim 1, is characterized in that in step 1) in, the light intensity in plastic box is less than 6lux, when light intensity is greater than 6lux, can increase the number of plies of sunshade net; The usage amount of described bleaching powder can be 250g.
5. the Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of a kind of indoor as claimed in claim 1, is characterized in that in step 1) in, the time of described sterilization is 24h; The described time of being exposed to the sun can be 1 ~ 2 day.
6. the Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of a kind of indoor as claimed in claim 1, is characterized in that in step 2) in (1) part, described seed chooses total length 3 ~ 4cm, the Bostrichthys sinensis seed of body weight 0.2 ~ 0.4g.
7. the Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of a kind of indoor as claimed in claim 1, is characterized in that in step 2) in (2) part, the quantity of described input seed puts into 15 tail seeds in each plastic box; The time of described morning and evening can be morning 8 and dusk 6 points; Described bait can adopt Articial bait making.
8. the Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of a kind of indoor as claimed in claim 1, it is characterized in that in step 2) in (3) part, residual bait in described removing plastic box and excremental method adopt siphon pipe to remove residual bait in plastic box and excreta, sunshade net can not be raised completely when carrying out this operation, extraneous light should be avoided as far as possible to irradiate and enter in plastic box; Described seed rearing water is changed and the seawater in plastic box can be changed 45% ~ 55%; Described seed rearing water is changed and is preferably selected between the lights or evening, avoids seed to be subject to the stimulation of high light.
9. the Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of a kind of indoor as claimed in claim 1, it is characterized in that in step 2) in (3) part, the concrete grammar that described seed rearing water is changed is: get out the clean seawater consistent with plastic box maritime interior waters salinity, first with siphon pipe by the seawater sucking-off 45% ~ 55% in plastic box, then add the ready clean seawater of equal volume.
10. the Bostrichthys sinensis seed continental rise controlled water body intermediate cultivate method of a kind of indoor as claimed in claim 1, is characterized in that in step 2) in (4) part, the time of intermediate rearing is 50 days.
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