CN104396478A - Method for improving brassica napus microspore cultivation test-tube plantlet field transplanting survival rate - Google Patents
Method for improving brassica napus microspore cultivation test-tube plantlet field transplanting survival rate Download PDFInfo
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- CN104396478A CN104396478A CN201410517196.1A CN201410517196A CN104396478A CN 104396478 A CN104396478 A CN 104396478A CN 201410517196 A CN201410517196 A CN 201410517196A CN 104396478 A CN104396478 A CN 104396478A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The invention discloses a method for improving brassica napus microspore cultivation test-tube plantlet field transplanting survival rate. The method comprises the steps of preparation before transplanting, field preparation, and transplanting. According to the invention, after brassica napus microspore in-vitro cultivation, seedlings induced from embryoids are subjected to test-tube plantlet transferring; when field transplanting is to be carried out, the test-tube plantlets are directly transplanted into the field. With the method, seedling-hardening time can be reduced, labor cost can be saved, robust seedlings can be provided for transplanting, and seedling survival rate after transplanting can be greatly improved. The method provided by the invention is simple, and has good effect. With the method, rape genetic breeding research and novel variety breeding processes can be promoted.
Description
Technical field
The present invention relates to field of agricultural sciences, especially a kind of method improving Microspore of Brassica napus culture test tube seedling field-transplanting survival rate.
Background technology
Cabbage type rape (
brassica napus L.) be the kind that grain yield is the highest in three kinds of oil rapes (turnip type rape, mustard type rape, cabbage type rape), planting in a large number in basin, China middle and lower reach of Yangtze River at present, is the important oil crop of China.
Cabbage type rape is important oil crop, not only can produce edible oil, and its grouts are rich in protein, can be used as animal feed.Because of containing a large amount of anti-nutrient substance in rape seed, as polyphenolic substance, lignin and cellulose etc., wherein polyphenol substance mainly comprises the derivative and glucosinolate etc. of tannin, proanthocyanidin, flavonoids, hydroxy-cinnamic acid, and these materials can have a strong impact on the quality of rapeseed oil and the nutritive value of feed.
Along with improving constantly of people's living standard, edible oil demand is continued to increase, and the improving constantly of the popularization of cabbage type rape variety and culture technique level, make China's Rape-seed production, in area, output and benefit, bring up to a new historical level, so, the high-yield culturing of cabbage type rape, to select improved seeds, nurturing staff, should apply fertilizers scientifically, enhance field management, make great efforts the output and the benefit that improve rape.In the transplantation of seedlings of Microspore of Brassica napus culture test tube, common microspores culture test-tube seedling transplanting mode survival rate is low, directly has influence on genetic breeding process and quality rape breed breeding.
Summary of the invention
The object of the invention is: after Microspore of Brassica napus cultured in vitro, the seedling that embryoid derives is transferred through test-tube plantlet, when field-transplanting, directly by the mode of test-tube seedling transplanting to land for growing field crops, reduce hardening link and labor cost, improve transplanting survival rate.
The present invention is achieved in that the method improving Microspore of Brassica napus culture test tube seedling field-transplanting survival rate, it is characterized in that: comprise the following steps:
1) prepare before transplanting: by the test-tube plantlet of aseptic culture, first 48 hours of transplanting by the cover lifting of test-tube plantlet, other environment is constant, continues cultivation 24 hours; Then the blake bottle without lid that test-tube plantlet is housed is placed into normal temperature environment, shady and cool without burning sun irradiation, incubated overnight cultivates 24 hours, sprays fog when seeing that blade withers to blade, within every 8-12 hour, sprays once, until transplant;
2) land for growing field crops prepares: land for growing field crops is opened railway carriage or compartment according to line-spacing 40cm, nest beat nest apart from 30cm, and N/P/K composite fertilizer executed by base fertilizer, survives rear applications of farmyard manure, first time use after every 30 days applications of farmyard manure again, conveniently manage afterwards;
3) transplant: seedling is directly taken out with tweezers from blake bottle, after rinsing agar and sugar well with water, is directly transplanted to land for growing field crops.
The fog sprayed once to blade for every 8-12 hour described in step 1) is running water, and till seeing that blade all drenches, without withering, phenomenon is not then sprayed.
Step 2) described in land for growing field crops prepare in base fertilizer Zhi Shi N/P/K composite fertilizer, consumption is 45-55 kg/acre.
Step 2) described in rear the used farmyard manure that survives be clear liquid dung, 900-1000ml used by every nest.
When described step 3) is transplanted, transplant under burning sun in summer, transplanted rear atomizers spray fog, soaked completely by soil surface 5-10cm layer, blade squirts completely, takes ground floor with shed film, every 3 meters of sides stay air vent, both ends open, and upper layer takes sunshade net, then according to 1 hour, 1.5 hour, 2 hours, 2 hours, 3 hours, 4 h apart times sprayed fog to blade, hide with sunshade net after carrying out spraying sprinkling fog according to every mu of 15L, until sunshine is decorporated completely at dusk, open sunshade net, shed film two ends continue to open wide by evening, the air vent of side is closed, next day is before the sun out, open the air vent of side, fog is sprayed to blade, upper soll layer 5-10cm waters permeable, afterwards in conjunction with temperature height, as long as blade starts to wither, spray fog immediately, until sunshine is decorporated completely at dusk, within 3-7 days, repeat the way of the 2nd day, after one week, test-tube plantlet survives substantially completely.
When described step 3) is transplanted, at the cloudy day in summer, the time interval of spraying fog is 3-4 hour.
Owing to have employed technique scheme, compared with prior art, after the present invention adopts Microspore of Brassica napus cultured in vitro, the seedling that embryoid derives is transferred through test-tube plantlet, when field-transplanting, directly by the mode of test-tube seedling transplanting to land for growing field crops, the hardening time can not only be reduced, to take things easy power cost, for providing healthy and strong seedling when transplanting, greatly can also improve the survival rate of seedling after transplanting, the inventive method is simple, effective, can promote that genetics breeding of rape is studied and selecting process for new fuchsin.
Embodiment
Embodiments of the invention 1: the method improving Microspore of Brassica napus culture test tube seedling field-transplanting survival rate, comprises the following steps:
1) prepare before transplanting: by the test-tube plantlet of aseptic culture, first 48 hours of transplanting by the cover lifting of test-tube plantlet, other environment is constant, continues cultivation 24 hours; Then the blake bottle without lid that test-tube plantlet is housed is placed into normal temperature environment, shady and cool without burning sun irradiation, incubated overnight cultivates 24 hours, sees that blade sprays fog to blade when starting to wither, and within every 8 hours, spray once, without withering, phenomenon is not then sprayed, until transplant;
2) land for growing field crops prepares: land for growing field crops is opened railway carriage or compartment according to line-spacing 40cm, nest apart from 30cm, often row 5 nest and beats nest, base fertilizer Zhi Shi N/P/K composite fertilizer consumption 45 kilograms, base fertilizer does not use farmyard manure, survive the clear liquid dung of rear applications of farmyard manure, 900ml used by every nest, first time use after every 30 days applications of farmyard manure again, conveniently manage afterwards.
3) transplant: seedling is directly taken out with tweezers from blake bottle, after rinsing fine jade and sugar fat well with water, is directly transplanted to land for growing field crops; At summer high temperature burning sun, transplanted rear atomizers spray fog, soaked completely by soil surface 5cm layer, blade squirts completely, then takes ground floor with shed film, and every 3 meters of sides stay air vent, both ends open, and upper layer takes sunshade net; Then according to 1 hour, 1.5 hours, 2 hours, 2 hours, 3 hours, 4 h apart times sprayed fog to blade, noon the highest temperature or burning sun irradiate all spray fog, spray according to every mu of 15L, hide with sunshade net after spraying fog, until sunshine is decorporated completely at dusk, open sunshade net, after transplanting, shed film two ends continue to open wide by evening, and side air vent is closed, improve temperature in railway carriage or compartment, promote root growth.Next day, before the sun out, opens side air vent, and spray fog to blade equally, upper soll layer 5cm waters permeable, afterwards in conjunction with temperature height, as long as blade starts to wither, sprays fog immediately, until sunshine is decorporated completely at dusk, repeats the way day before yesterday.3rd day to the 7th day, according to the management method management of second day, after one week, test-tube plantlet survived substantially completely, the survival rate 92% of transplanting.
Embodiments of the invention 2: the method improving Microspore of Brassica napus culture test tube seedling field-transplanting survival rate, comprises the following steps:
Prepare before transplanting: by the test-tube plantlet of aseptic culture, first 48 hours of transplanting by the cover lifting of test-tube plantlet, other environment is constant, continues cultivation 24 hours; Then the blake bottle without lid that test-tube plantlet is housed is placed into normal temperature environment, shady and cool without burning sun irradiation, incubated overnight cultivates 24 hours, sees that blade sprays fog to blade when starting to wither, and within every 12 hours, spray once, without withering, phenomenon is not then sprayed, until transplant;
Land for growing field crops prepares: land for growing field crops is opened railway carriage or compartment according to line-spacing 40cm, nest apart from 30cm, often row 5 nest and beats nest, base fertilizer Zhi Shi N/P/K composite fertilizer consumption 45 kilograms, base fertilizer does not use farmyard manure, survive the clear liquid dung of rear applications of farmyard manure, 1000ml used by every nest, absolutely not should be many, first time use after every 30 days applications of farmyard manure again, conveniently manage afterwards.
During transplanting, seedling is directly taken out with tweezers from blake bottle, after rinsing agar and sugar well with water, is directly transplanted to land for growing field crops; At the cloudy day in summer, transplanted rear atomizers spray fog, soaked completely by soil surface 10cm layer, blade squirts completely, then takes ground floor with shed film, and every 3 meters of sides stay air vent, both ends open, and upper layer takes sunshade net; Then every compartment sprays fog to blade in 3 hours, spray according to every mu of 15L, noon the highest temperature or burning sun irradiate and all spray fog, spray after fog and hide with sunshade net, until sunshine is decorporated completely at dusk, open sunshade net, after transplanting, shed film two ends continue to open wide by evening, and side air vent is closed, improve temperature in railway carriage or compartment, promote root growth.Next day, before the sun out, opens side air vent, and spray fog to blade equally, upper soll layer 10cm waters permeable, afterwards in conjunction with temperature height, as long as blade starts to wither, sprays fog immediately, until sunshine is decorporated completely at dusk, repeats the way day before yesterday.3rd day to the 7th day, according to the management method management of second day, after one week, test-tube plantlet survived substantially completely, the survival rate 95% of transplanting.
Embodiments of the invention 3: the method improving Microspore of Brassica napus culture test tube seedling field-transplanting survival rate, comprises the following steps:
Prepare before transplanting: by the test-tube plantlet of aseptic culture, first 48 hours of transplanting by the cover lifting of test-tube plantlet, other environment is constant, continues cultivation 24 hours; Then the blake bottle without lid that test-tube plantlet is housed is placed into normal temperature environment, shady and cool without burning sun irradiation, incubated overnight cultivates 24 hours, sees that blade sprays fog to blade when starting to wither, and within every 12 hours, spray once, without withering, phenomenon is not then sprayed, until transplant;
Land for growing field crops prepares: land for growing field crops is opened railway carriage or compartment according to line-spacing 40cm, nest apart from 30cm, often row 5 nest and beats nest, base fertilizer Zhi Shi N/P/K composite fertilizer consumption 45 kilograms, base fertilizer does not use farmyard manure, survive the clear liquid dung of rear applications of farmyard manure, 1000ml used by every nest, absolutely not should be many, first time use after every 30 days applications of farmyard manure again, conveniently manage afterwards.
During transplanting, seedling is directly taken out with tweezers from blake bottle, after rinsing agar and sugar well with water, is directly transplanted to land for growing field crops; At the cloudy day in summer, transplanted rear atomizers spray fog, soaked completely by soil surface 5cm layer, blade squirts completely, then takes ground floor with shed film, and every 3 meters of sides stay air vent, both ends open, and upper layer takes sunshade net; Then every compartment sprays fog to blade in 3 hours, spray according to every mu of 15L, noon the highest temperature or burning sun irradiate and all spray fog, spray after fog and hide with sunshade net, until sunshine is decorporated completely at dusk, open sunshade net, after transplanting, shed film two ends continue to open wide by evening, and side air vent is closed, improve temperature in railway carriage or compartment, promote root growth.Next day, before the sun out, opens side air vent, and spray fog to blade equally, upper soll layer 10cm waters permeable, afterwards in conjunction with temperature height, as long as blade starts to wither, sprays fog immediately, until sunlight is decorporated completely at dusk, repeats the way day before yesterday.3rd day to the 7th day, according to the management method management of second day, after one week, test-tube plantlet survived substantially completely, the survival rate 93% of transplanting.
Embodiment 1, embodiment 2 and embodiment 3 are the result of the test of 3 years respectively: in June, 2011, Chongqing Xiannushan transplanted 275 strains, survived 253 strains, survival rate 92%; Within 2012, Beibei, chongqing transplants 303 strains, survives 288 strains, survival rate 95%; Within 2013,230 strains are transplanted in Kweiyang, survive 214 strains, survival rate 93%.
Claims (6)
1. improve the method for Microspore of Brassica napus culture test tube seedling field-transplanting survival rate, it is characterized in that: comprise the following steps:
1) prepare before transplanting: by the test-tube plantlet of aseptic culture, first 48 hours of transplanting by the cover lifting of test-tube plantlet, other environment is constant, continues cultivation 24 hours; Then the blake bottle without lid that test-tube plantlet is housed is placed into normal temperature environment, shady and cool without burning sun irradiation, incubated overnight cultivates 24 hours, sees that blade sprays fog to blade when starting to wither, and within every 8-12 hour, sprays once, until transplant;
2) land for growing field crops prepares: land for growing field crops is opened railway carriage or compartment according to line-spacing 40cm, nest beat nest apart from 30cm, and N/P/K composite fertilizer executed by base fertilizer, to the seedling applications of farmyard manure after surviving, first time use after every 30 days applications of farmyard manure again, conveniently manage afterwards;
3) transplant: seedling is directly taken out with tweezers from blake bottle, after rinsing agar and sugar well with water, is directly transplanted to land for growing field crops.
2. the method for raising Microspore of Brassica napus culture test tube seedling field-transplanting survival rate according to claim 1, is characterized in that: the fog sprayed once to blade for every 8-12 hour described in step 1) is running water, till seeing that blade all drenches; Without withering, phenomenon is not then sprayed.
3. the method for raising Microspore of Brassica napus culture test tube seedling field-transplanting survival rate according to claim 1, is characterized in that: step 2) described in land for growing field crops prepare in base fertilizer Zhi Shi N/P/K composite fertilizer, consumption is 45-55 kg/acre.
4. the method for raising Microspore of Brassica napus culture test tube seedling field-transplanting survival rate according to claim 1, is characterized in that: step 2) described in survive rear used farmyard manure be clear liquid dung, 900-1000ml used by every nest.
5. the method for raising Microspore of Brassica napus culture test tube seedling field-transplanting survival rate according to claim 1, it is characterized in that: when described step 3) is transplanted, transplant under burning sun in summer, transplanted rear atomizers spray fog, soaked completely by soil surface 5-10cm layer, blade squirts completely, ground floor is taken with shed film, air vent is stayed in side, both ends open, and upper layer takes sunshade net; Then according to 1 hour, 1.5 hours, 2 hours, 2 hours, 3 hours, 4 h apart times sprayed fog to blade, spray according to 15L/ mu; Hide with sunshade net after spraying fog, until sunshine is decorporated completely at dusk, open sunshade net, shed film two ends continue to open wide by evening, and closed by the air vent of side, next day is before the sun out, open the air vent of side, spray fog to blade, upper soll layer 5-10cm waters permeable, afterwards in conjunction with temperature height, as long as blade starts to wither, spray fog immediately, until sunshine is decorporated completely at dusk, within 3-7 days, repeat the way of the 2nd day.
6. the method for raising Microspore of Brassica napus culture test tube seedling field-transplanting survival rate according to claim 5, is characterized in that: when described step 3) is transplanted, and at the cloudy day in summer, the time interval of spraying fog is 3-4 hour.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107548925A (en) * | 2016-06-23 | 2018-01-09 | 镇江三龙生态农业发展有限公司 | A kind of cultural method of spore broccoli |
| CN109169132A (en) * | 2018-09-27 | 2019-01-11 | 岭南生态文旅股份有限公司 | A kind of Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait tissue-cultured seedling crop field efficient cultivation method |
| CN113080065A (en) * | 2021-05-19 | 2021-07-09 | 南京农业大学 | Method for cultivating non-heading Chinese cabbage microspore plant |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107548925A (en) * | 2016-06-23 | 2018-01-09 | 镇江三龙生态农业发展有限公司 | A kind of cultural method of spore broccoli |
| CN109169132A (en) * | 2018-09-27 | 2019-01-11 | 岭南生态文旅股份有限公司 | A kind of Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait tissue-cultured seedling crop field efficient cultivation method |
| CN113080065A (en) * | 2021-05-19 | 2021-07-09 | 南京农业大学 | Method for cultivating non-heading Chinese cabbage microspore plant |
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