CN104374615A - Schizothoracinae otolith slice production method - Google Patents
Schizothoracinae otolith slice production method Download PDFInfo
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- CN104374615A CN104374615A CN201410471796.9A CN201410471796A CN104374615A CN 104374615 A CN104374615 A CN 104374615A CN 201410471796 A CN201410471796 A CN 201410471796A CN 104374615 A CN104374615 A CN 104374615A
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Abstract
The invention discloses a Schizothoracinae otolith slice production method, and relates to a fish otolith slice production method. A purpose of the present invention is to solve problems of single material use, complex operation, time consuming and difficult operation of the existing Schizothoracinae otolith slice production method. The production method of the present invention comprises: 1, removing otolith, 2, soaking the otolith, 3, washing the otolith, 4, fixing the otolith, 5, grinding the slice, and 6,sealing the slice to obtain the clear Schizothoracinae otolith slice capable of being stored for a long time. According to the present invention, the Schizothoracinae otolith slice production method is provided.
Description
Technical field
The present invention relates to the method for making of a kind of Fish otolith section.
Background technology
Fish otolith is present in fish body inner ear membranous labyrinth, and it is three right to have, and forms, physiology plays a part the sense of hearing and balance primarily of carbonate mineral matter and a small amount of organic and trace element.Otolith obtains the concern of ichthyologist very early, is the effective way as Age Determination material the earliest, than the more accurately readability of the annual ring on scale.Since the Pannella Late Cambrian sun in 1971, the existing a large amount of report of the various correlative studys utilizing the sun to carry out, otolith was once becoming the focus of research.
Otolith, as life mineral, has the good characteristic of life entity and mineral matter concurrently.It not only records fish growth and the relevant information in the history of life, but also indicates present situation and the transition of its Living Water environment, and these data persistences, once formation just presents biologically inert, are got off by the microstructure on it and micro-chemical species by otolith.The characteristic of otolith medium trace element is combined research with the sun, effectively can solve a lot of ecology of fishes problem.At present, take otolith as all many-sides that application that medium carries out information analysis covers ecology of fishes research, as fish hatching and early development growth, spawning ground and egg-laying season judge, Age Determination and age identification, individual migration etc.And along with the development of correlation theory and technology, become a kind of and disclose fish history of life, the history of life rebuilds and water body environment territory becomes gordian technique.
Be in the process of the above-mentioned Scientific Research Problem of investigation of materials with otolith, in order to obtain otolith annual ring or the sun clearly, will be examined under a microscope by the section of making otolith, taking pictures obtains.The manufacturing process more complicated of otolith section, different Fish otolith has the feature of its uniqueness, and the method for making of otolith section is not quite similar.Adopted merely the method for the curing materials such as nail polish, hardening resin, hot melt adhesive embedding otolith then abrasive disc, more complicated in whole journey, consuming time longer, effect is not ideal enough in the past.When wherein adopting merely nail polish to grind otolith section, often there is following problem: (1) uses nail polish to fix otolith and ground one side and then overturn mill otolith another side, and use nail polish embedding otolith in process for twice, set time is long; (2) the micro-otolith of schizothoracin class is thicker, and nail polish is difficult to it to cover completely; (3) adopt acetone solution nail polish then to be taken out by otolith after having ground otolith one side, again fix with nail polish, another side of regrinding after it solidifies completely.This process is consuming time longer, and easily causes mechanical damage to otolith in otolith switching process.And use merely epoxy resin to add rigidizer also to there are some problems, when another side being polished after otolith one side has ground, especially when otolith abrasive disc is thinner, otolith abrasive disc is not easily affectedly bashful, inconvenient operation when grinding further, and now abrasive disc is thinner very easily fractures, next step operation is made troubles.
Summary of the invention
It is single to there is the material that institute is in the method that the object of the invention is to solve the section of existing making schizothoracin class otolith, complicated operation, length consuming time and the problem that should not operate, and provides the method for making that a kind of schizothoracin class otolith is cut into slices.
A method for making for schizothoracin class otolith section, specifically completes according to the following steps:
One, otolith is won: use scalper and elbow tweezers to be taken out by schizothoracin class otolith, then reject the dirt on schizothoracin class otolith surface;
Two, soak otolith: be placed in 2mL ~ 5mL centrifuge tube by rejecting the schizothoracin class otolith of surface smut, then add alcohol-pickled schizothoracin class otolith 2d ~ 5d that volume fraction is 95%, obtain alcohol-pickled after schizothoracin class otolith;
Three, clean otolith: 2 times ~ 3 times, the schizothoracin class otolith surface after using distilled water flushing alcohol-pickled, more at room temperature dry, obtain the schizothoracin class otolith after drying;
Four, fixing otolith: be first coated with one deck release agent in the bottom and sidewall of mould of plastics, then step 3 is obtained dry after schizothoracin class otolith concave surface down convex surface be positioned over upward in mould of plastics, on schizothoracin class otolith after again resin-cast being dried in mould of plastics, when the schizothoracin class otolith after drying embeds by resin completely, stop cast, at room temperature place 12h ~ 15h again, again the hardening resin block of parcel schizothoracin class otolith is taken out from mould of plastics, obtain the hardening resin block being embedded with schizothoracin class otolith;
Five, abrasive disc: use 500# ~ 600# sand paper and 1000# ~ 1200# sand paper to polish to that face being embedded with schizothoracin class otolith concave surface that is embedded with in the hardening resin block of schizothoracin class otolith that step 4 obtains 1., successively, often polish after 5s ~ 6s and examine under a microscope its polishing degree, until polishing is to the core place of schizothoracin class otolith, obtain the schizothoracin class otolith after polishing; 2., re-use polishing paper and polishing is carried out to the schizothoracin class otolith concave surface after polishing, and then use distilled water to carry out cleaning 2 times ~ 3 times, at room temperature dry again, and then use transparent nail polish to be fixed on microslide by schizothoracin class otolith concave surface, then microslide is put into the dry 20h ~ 24h of baking oven that temperature is 30 DEG C ~ 35 DEG C; 3., hand-held microslide, 500# ~ 600# sand paper and 1000# ~ 1200# sand paper is used to polish to that face hardening resin being embedded with schizothoracin class otolith convex surface of not polishing successively, often polish after 5s ~ 6s and examine under a microscope its polishing degree, until observe schizothoracin class otolith wheel line clearly, stop polishing, obtain the schizothoracin class otolith abrasive disc that thickness is 0.4mm ~ 0.7mm;
Six, mounting: use polishing paper to carry out polishing to the schizothoracin class otolith abrasive disc that thickness is 0.4mm ~ 0.7mm, re-use distilled water and cleaning is carried out 2 times ~ 3 times to the schizothoracin class otolith abrasive disc after polishing, at room temperature dry again, instill 1 ~ 2 transparent nail polish again and cover the mill of the schizothoracin class otolith after polishing, after drying under room temperature, obtain the class of schizothoracin clearly otolith with long preservation period section.
The present invention can obtain the method for making of a kind of schizothoracin class otolith section.
Advantage of the present invention: single use nail polish or single hardened material in the process of existing making otolith section, when only using nail polish as curing materials, nail polish setting time is slow on the one hand, general more than 2 days, grinding one side on the other hand, carrying out overturning grinds in the process of another side, need acetone solution nail polish, then otolith is turned and carry out next step sanding operation, otolith is easily damaged in the process, and to otolith be cleared up when again embedding with nail polish, process is comparatively complicated; Simple use curing materials, during as otolith sections processed such as hardening resins, although turning operation when grinding unlike single use nail polish, but when one side polishing end grinds otolith another side, especially when abrasive disc is thinner, not easily hold when on the one hand abrasive disc does not have attachment to grind, operation inconvenience, on the other hand thin slice very fragilely very easily to fracture, broken; The invention solves the existing otolith section easily breakage existed in the section of making otolith, complicated operation and the long problem that expends time in, the present invention saves 20% ~ 30% relative to existing method on the making otolith section time; Operate more convenient, damage can not be caused to otolith, therefore, one aspect of the present invention uses epoxy resin embedding otolith to have to save the advantages such as setting time, coagulation result are good, on the other hand the abrasive disc of having polished is fixed on microslide and carries out next step operation, there is the advantage of set abrasive disc, operating aspect.
Accompanying drawing explanation
Fig. 1 is the different fissure abdomen Fish otolith abrasive disc figure that test one step 5 obtains that thickness is 0.6mm, and in Fig. 1,1 is different fissure abdomen Fish otolith, and 2 is the resin hardened, and 3 is the transparent nail polish solidified;
Fig. 2 is the different fissure abdomen Fish otolith slice map clearly with long preservation period that test one step 6 obtains; In Fig. 2,1 is different fissure abdomen Fish otolith, and 2 is the resin hardened, and 3 is the transparent nail polish solidified;
Fig. 3 is that to obtain thickness be that the different fissure abdomen Fish otolith abrasive disc of 0.6mm amplifies picture under the microscope to test one step 5.
Embodiment
Embodiment one: the method for making that a kind of schizothoracin class otolith is cut into slices specifically completes according to the following steps:
One, otolith is won: use scalper and elbow tweezers to be taken out by schizothoracin class otolith, then reject the dirt on schizothoracin class otolith surface;
Two, soak otolith: be placed in 2mL ~ 5mL centrifuge tube by rejecting the schizothoracin class otolith of surface smut, then add alcohol-pickled schizothoracin class otolith 2d ~ 5d that volume fraction is 95%, obtain alcohol-pickled after schizothoracin class otolith;
Three, clean otolith: 2 times ~ 3 times, the schizothoracin class otolith surface after using distilled water flushing alcohol-pickled, more at room temperature dry, obtain the schizothoracin class otolith after drying;
Four, fixing otolith: be first coated with one deck release agent in the bottom and sidewall of mould of plastics, then step 3 is obtained dry after schizothoracin class otolith concave surface down convex surface be positioned over upward in mould of plastics, on schizothoracin class otolith after again resin-cast being dried in mould of plastics, when the schizothoracin class otolith after drying embeds by resin completely, stop cast, at room temperature place 12h ~ 15h again, again the hardening resin block of parcel schizothoracin class otolith is taken out from mould of plastics, obtain the hardening resin block being embedded with schizothoracin class otolith;
Five, abrasive disc: use 500# ~ 600# sand paper and 1000# ~ 1200# sand paper to polish to that face being embedded with schizothoracin class otolith concave surface that is embedded with in the hardening resin block of schizothoracin class otolith that step 4 obtains 1., successively, often polish after 5s ~ 6s and examine under a microscope its polishing degree, until polishing is to the core place of schizothoracin class otolith, obtain the schizothoracin class otolith after polishing; 2., re-use polishing paper and polishing is carried out to the schizothoracin class otolith concave surface after polishing, and then use distilled water to carry out cleaning 2 times ~ 3 times, at room temperature dry again, and then use transparent nail polish to be fixed on microslide by schizothoracin class otolith concave surface, then microslide is put into the dry 20h ~ 24h of baking oven that temperature is 30 DEG C ~ 35 DEG C; 3., hand-held microslide, 500# ~ 600# sand paper and 1000# ~ 1200# sand paper is used to polish to that face hardening resin being embedded with schizothoracin class otolith convex surface of not polishing successively, often polish after 5s ~ 6s and examine under a microscope its polishing degree, until observe schizothoracin class otolith wheel line clearly, stop polishing, obtain the schizothoracin class otolith abrasive disc that thickness is 0.4mm ~ 0.7mm;
Six, mounting: use polishing paper to carry out polishing to the schizothoracin class otolith abrasive disc that thickness is 0.4mm ~ 0.7mm, re-use distilled water and cleaning is carried out 2 times ~ 3 times to the schizothoracin class otolith abrasive disc after polishing, at room temperature dry again, instill 1 ~ 2 transparent nail polish again and cover the mill of the schizothoracin class otolith after polishing, after drying under room temperature, obtain the class of schizothoracin clearly otolith with long preservation period section.
Release agent described in present embodiment step 4 is silicone oil, and buy from Si Teer company, production code member is: 40300076;
Mould of plastics described in present embodiment step 4 is bought from Si Teer company, production code member 40300085, and the internal diameter of mould of plastics is 1cm ~ 2cm;
The nail polish used in present embodiment step 5 and step 6 is commercially available normal transparent nail polish.
Present embodiment can obtain the method for making of a kind of schizothoracin class otolith section.
The advantage of present embodiment: single use nail polish or single hardened material in the process of existing making otolith section, when only using nail polish as curing materials, nail polish setting time general more than 2 days slowly on the one hand, grinding one side on the other hand, carrying out overturning grinds in the process of another side, need acetone solution nail polish, then otolith is turned and carry out next step sanding operation, otolith is easily damaged in the process, and to otolith be cleared up when again embedding with nail polish, process is comparatively complicated; Simple use curing materials, during as making otolith sections such as hardening resins, although turning operation when grinding unlike single use nail polish, but when one side polishing end grinds otolith another side, especially when abrasive disc is thinner, not easily hold when on the one hand abrasive disc does not have attachment to grind, operation inconvenience, on the other hand thin slice very fragilely very easily to fracture, broken; Present embodiment solves the existing otolith section easily breakage existed in the section of making otolith, complicated operation and the long problem that expends time in, and present embodiment saves 20% ~ 30% relative to existing method on the making otolith section time; Operate more convenient, damage can not be caused to otolith, therefore, present embodiment uses on the one hand epoxy resin embedding otolith to have to save the advantages such as setting time, coagulation result are good, on the other hand the abrasive disc of having polished is fixed on microslide and carries out next step operation, there is the advantage of set abrasive disc, operating aspect.
Embodiment two: present embodiment with embodiment one difference is: the preparation method of the resin described in step 4 is as follows: epoxy resin and rigidizer are mixed, low whipping speed is stir 2min ~ 3min under 50r/min ~ 60r/min, leave standstill 2min ~ 3min again, obtain resin; Described epoxy resin and the mass ratio of rigidizer are 25:3.Other steps are identical with embodiment one.
Epoxy resin described in present embodiment buys the EpoFix resin produced in Si Teer company (Struers), production code member 40200029; Described rigidizer is the EpoFix rigidizer matching used with EpoFix resin-phase that Si Teer company (Struers) produces, and production code member is also 40200029.
Embodiment three: one of present embodiment and embodiment one or two difference is: in step 2, the schizothoracin class otolith of rejecting surface smut is placed in 2mL ~ 3mL centrifuge tube, add alcohol-pickled schizothoracin class otolith 2d ~ 3d that volume fraction is 95% again, obtain alcohol-pickled after schizothoracin class otolith.Other steps are identical with embodiment one or two.
Embodiment four: one of present embodiment and embodiment one to three difference is: in step 2, the schizothoracin class otolith of rejecting surface smut is placed in 4mL ~ 5mL centrifuge tube, add alcohol-pickled schizothoracin class otolith 4d ~ 5d that volume fraction is 95% again, obtain alcohol-pickled after schizothoracin class otolith.Other steps are identical with embodiment one to three.
Embodiment five: one of present embodiment and embodiment one to four difference is: the release agent described in step 4 is silicone oil.Other steps are identical with embodiment one to four.
Embodiment six: one of present embodiment and embodiment one to five difference is: be first coated with one deck release agent in the bottom and sidewall of mould of plastics in step 4, then step 3 is obtained dry after schizothoracin class otolith concave surface down convex surface be positioned over upward in mould of plastics, on schizothoracin class otolith after again resin-cast being dried in mould of plastics, when the schizothoracin class otolith after drying embeds by resin completely, stop cast, at room temperature place 14h ~ 15h again, again the hardening resin block of parcel schizothoracin class otolith is taken out from mould of plastics, obtain the hardening resin block being embedded with schizothoracin class otolith.Other steps are identical with embodiment one to five.
Embodiment seven: one of present embodiment and embodiment one to six difference is: step 5 1. in use 500# ~ 600# sand paper and 1000# ~ 1200# sand paper to polish to that face being embedded with schizothoracin class otolith concave surface that is embedded with in the hardening resin block of schizothoracin class otolith that step 4 obtains successively, often examine under a microscope its polishing degree after polishing 5s, until polishing is to the core place of schizothoracin class otolith, obtain the schizothoracin class otolith after polishing.Other steps are identical with embodiment one to six.
Embodiment eight: one of present embodiment and embodiment one to seven difference is: the step 5 2. middle polishing paper that uses carries out polishing to the schizothoracin class otolith concave surface after polishing, and then use distilled water to carry out cleaning 2 times ~ 3 times, at room temperature dry again, and then use transparent nail polish to be fixed on microslide by schizothoracin class otolith concave surface, then microslide is put into the dry 22h ~ 24h of baking oven that temperature is 33 DEG C ~ 35 DEG C.Other steps are identical with embodiment one to seven.
Embodiment nine: one of present embodiment and embodiment one to eight difference is: the step 5 2. middle polishing paper that uses carries out polishing to the schizothoracin class otolith concave surface after polishing, and then use distilled water to carry out cleaning 2 times ~ 3 times, at room temperature dry again, and then use transparent nail polish to be fixed on microslide by schizothoracin class otolith concave surface, then microslide is put into the dry 20h ~ 22h of baking oven that temperature is 30 DEG C ~ 32 DEG C.Other steps are identical with embodiment one to eight.
Embodiment ten: one of present embodiment and embodiment one to nine difference is: step 5 3. in hand-held microslide, 500# ~ 600# sand paper and 1000# ~ 1200# sand paper is used to polish to that face hardening resin being embedded with schizothoracin class otolith convex surface of not polishing successively, often examine under a microscope its polishing degree after polishing 5s, until observe schizothoracin class otolith wheel line clearly, stop polishing, obtain the schizothoracin class otolith abrasive disc that thickness is 0.4mm ~ 0.5mm.Other steps are identical with embodiment one to nine.
Adopt following verification experimental verification beneficial effect of the present invention:
A method for making for schizothoracin class otolith section, specifically completes according to the following steps:
One, otolith is won: use scalper and elbow tweezers to be taken out by schizothoracin class otolith, then reject the dirt on schizothoracin class otolith surface;
Two, soak otolith: be placed in 3mL centrifuge tube by rejecting the schizothoracin class otolith of surface smut, then add the alcohol-pickled schizothoracin class otolith 2d that volume fraction is 95%, obtain alcohol-pickled after schizothoracin class otolith;
Three, clean otolith: 3 times, the schizothoracin class otolith surface after using distilled water flushing alcohol-pickled, more at room temperature dry, obtain the schizothoracin class otolith after drying;
Four, fixing otolith: be first coated with one deck release agent in the bottom and sidewall of mould of plastics, then step 3 is obtained dry after schizothoracin class otolith concave surface down convex surface be positioned over upward in mould of plastics, on schizothoracin class otolith after again resin-cast being dried in mould of plastics, when the schizothoracin class otolith after drying embeds by resin completely, stop cast, at room temperature place 14h again, again the hardening resin block of parcel schizothoracin class otolith is taken out from mould of plastics, obtain the hardening resin block being embedded with schizothoracin class otolith;
Five, abrasive disc: use 500# ~ 600# sand paper and 1000# ~ 1200# sand paper to polish to that face being embedded with schizothoracin class otolith concave surface that is embedded with in the hardening resin block of schizothoracin class otolith that step 4 obtains 1., successively, often examine under a microscope its polishing degree after polishing 5s, until polishing is to the core place of schizothoracin class otolith, obtain the schizothoracin class otolith after polishing; 2., re-use polishing paper and polishing is carried out to the schizothoracin class otolith concave surface after polishing, and then use distilled water to carry out cleaning 3 times, at room temperature dry again, and then use transparent nail polish to be fixed on microslide by schizothoracin class otolith concave surface, then microslide is put into the dry 22h of baking oven that temperature is 33 DEG C; 3., hand-held microslide, 500# ~ 600# sand paper and 1000# ~ 1200# sand paper is used to polish to that face hardening resin being embedded with schizothoracin class otolith convex surface of not polishing successively, often examine under a microscope its polishing degree after polishing 5s, until observe schizothoracin class otolith wheel line clearly, stop polishing, obtain the schizothoracin class otolith abrasive disc that thickness is 0.6mm;
Six, mounting: use polishing paper to carry out polishing to the schizothoracin class otolith abrasive disc that thickness is 0.6mm, re-use distilled water and cleaning is carried out 3 times to the schizothoracin class otolith abrasive disc after polishing, at room temperature dry again, instill 2 transparent nail polish again and cover the mill of the schizothoracin class otolith after polishing, after drying under room temperature, obtain the class of schizothoracin clearly otolith with long preservation period section.
Schizothoracin class described in this test procedure one is different fissure abdomen fish.
The advantage of this test: single use nail polish or single hardened material in the process of existing making otolith section, when only using nail polish as curing materials, nail polish setting time is slow on the one hand, general more than 2 days, grinding one side on the other hand, carrying out overturning grinds in the process of another side, need acetone solution nail polish, then otolith is turned and carry out next step sanding operation, otolith is easily damaged in the process, and to otolith be cleared up when again embedding with nail polish, process is comparatively complicated; Simple use curing materials, during as making otolith sections such as hardening resins, although turning operation when grinding unlike single use nail polish, but when one side polishing end grinds otolith another side, especially when abrasive disc is thinner, not easily hold when on the one hand abrasive disc does not have attachment to grind, operation inconvenience, on the other hand thin slice very fragilely very easily to fracture, broken; This test solves the existing otolith section easily breakage existed in the section of making otolith, complicated operation and the long problem that expends time in, and this test saves 30% relative to existing method on the making otolith section time; Operate more convenient, damage can not be caused to otolith, therefore, this test uses on the one hand epoxy resin embedding otolith to have to save the advantages such as setting time, coagulation result are good, on the other hand the abrasive disc of having polished is fixed on microslide and carries out next step operation, there is the advantage of set abrasive disc, operating aspect.
Digital Video is used to take pictures with the different fissure abdomen Fish otolith section clearly with long preservation period that test one step 6 obtains to the schizothoracin class otolith abrasive disc that the thickness that test one step 5 obtains is 0.6mm, as depicted in figs. 1 and 2, Fig. 1 is the different fissure abdomen Fish otolith abrasive disc figure that test one step 5 obtains that thickness is 0.6mm, in Fig. 1,1 is different fissure abdomen Fish otolith, 2 is the resin hardened, and 3 is the transparent nail polish solidified; Fig. 2 is the different fissure abdomen Fish otolith slice map clearly with long preservation period that test one step 6 obtains; In Fig. 2,1 is different fissure abdomen Fish otolith, and 2 is the resin hardened, and 3 is the transparent nail polish solidified.
Use microscope to observe the test one step 5 different fissure abdomen Fish otolith abrasive disc that to obtain thickness be 0.6mm, as shown in Figure 3, Fig. 3 is that to obtain thickness be that the different fissure abdomen Fish otolith abrasive disc of 0.6mm amplifies picture under the microscope to test one step 5.
Claims (10)
1. a method for making for schizothoracin class otolith section, is characterized in that method for making that a kind of schizothoracin class otolith is cut into slices specifically completes according to the following steps:
One, otolith is won: use scalper and elbow tweezers to be taken out by schizothoracin class otolith, then reject the dirt on schizothoracin class otolith surface;
Two, soak otolith: be placed in 2mL ~ 5mL centrifuge tube by rejecting the schizothoracin class otolith of surface smut, then add alcohol-pickled schizothoracin class otolith 2d ~ 5d that volume fraction is 95%, obtain alcohol-pickled after schizothoracin class otolith;
Three, clean otolith: 2 times ~ 3 times, the schizothoracin class otolith surface after using distilled water flushing alcohol-pickled, more at room temperature dry, obtain the schizothoracin class otolith after drying;
Four, fixing otolith: be first coated with one deck release agent in the bottom and sidewall of mould of plastics, then step 3 is obtained dry after schizothoracin class otolith concave surface down convex surface be positioned over upward in mould of plastics, on schizothoracin class otolith after again resin-cast being dried in mould of plastics, when the schizothoracin class otolith after drying embeds by resin completely, stop cast, at room temperature place 12h ~ 15h again, again the hardening resin block of parcel schizothoracin class otolith is taken out from mould of plastics, obtain the hardening resin block being embedded with schizothoracin class otolith;
Five, abrasive disc: use 500# ~ 600# sand paper and 1000# ~ 1200# sand paper to polish to that face being embedded with schizothoracin class otolith concave surface that is embedded with in the hardening resin block of schizothoracin class otolith that step 4 obtains 1., successively, often polish after 5s ~ 6s and examine under a microscope its polishing degree, until polishing is to the core place of schizothoracin class otolith, obtain the schizothoracin class otolith after polishing; 2., re-use polishing paper and polishing is carried out to the schizothoracin class otolith concave surface after polishing, and then use distilled water to carry out cleaning 2 times ~ 3 times, at room temperature dry again, and then use transparent nail polish to be fixed on microslide by schizothoracin class otolith concave surface, then microslide is put into the dry 20h ~ 24h of baking oven that temperature is 30 DEG C ~ 35 DEG C; 3., hand-held microslide, 500# ~ 600# sand paper and 1000# ~ 1200# sand paper is used to polish to that face hardening resin being embedded with schizothoracin class otolith convex surface of not polishing successively, often polish after 5s ~ 6s and examine under a microscope its polishing degree, until observe schizothoracin class otolith wheel line clearly, stop polishing, obtain the schizothoracin class otolith abrasive disc that thickness is 0.4mm ~ 0.7mm;
Six, mounting: use polishing paper to carry out polishing to the schizothoracin class otolith abrasive disc that thickness is 0.4mm ~ 0.7mm, re-use distilled water and cleaning is carried out 2 times ~ 3 times to the schizothoracin class otolith abrasive disc after polishing, at room temperature dry again, instill 1 ~ 2 transparent nail polish again and cover the mill of the schizothoracin class otolith after polishing, after drying under room temperature, obtain the class of schizothoracin clearly otolith with long preservation period section.
2. the method for making of a kind of schizothoracin class otolith section according to claim 1, it is characterized in that the preparation method of the resin described in step 4 is as follows: by epoxy resin and rigidizer mixing, low whipping speed is stir 2min ~ 3min under 50r/min ~ 60r/min, leave standstill 2min ~ 3min again, obtain resin; Described epoxy resin and the mass ratio of rigidizer are 25:3.
3. the method for making of a kind of schizothoracin class otolith section according to claim 1, it is characterized in that, in step 2, the schizothoracin class otolith of rejecting surface smut is placed in 2mL ~ 3mL centrifuge tube, add alcohol-pickled schizothoracin class otolith 2d ~ 3d that volume fraction is 95% again, obtain alcohol-pickled after schizothoracin class otolith.
4. the method for making of a kind of schizothoracin class otolith section according to claim 1, it is characterized in that, in step 2, the schizothoracin class otolith of rejecting surface smut is placed in 4mL ~ 5mL centrifuge tube, add alcohol-pickled schizothoracin class otolith 4d ~ 5d that volume fraction is 95% again, obtain alcohol-pickled after schizothoracin class otolith.
5. the method for making of a kind of schizothoracin class otolith section according to claim 1, is characterized in that the release agent described in step 4 is silicone oil.
6. the method for making of a kind of schizothoracin class otolith section according to claim 1, it is characterized in that first in the bottom and sidewall of mould of plastics, being coated with one deck release agent in step 4, then step 3 is obtained dry after schizothoracin class otolith concave surface down convex surface be positioned over upward in mould of plastics, on schizothoracin class otolith after again resin-cast being dried in mould of plastics, when the schizothoracin class otolith after drying embeds by resin completely, stop cast, at room temperature place 14h ~ 15h again, again the hardening resin block of parcel schizothoracin class otolith is taken out from mould of plastics, obtain the hardening resin block being embedded with schizothoracin class otolith.
7. the method for making of a kind of schizothoracin class otolith section according to claim 1, it is characterized in that during step 5 1., using 500# ~ 600# sand paper and 1000# ~ 1200# sand paper to polish to that face being embedded with schizothoracin class otolith concave surface that is embedded with in the hardening resin block of schizothoracin class otolith that step 4 obtains successively, often examine under a microscope its polishing degree after polishing 5s, until polishing is to the core place of schizothoracin class otolith, obtain the schizothoracin class otolith after polishing.
8. the method for making of a kind of schizothoracin class otolith section according to claim 1, it is characterized in that using polishing paper to carry out polishing to the schizothoracin class otolith concave surface after polishing during step 5 2., and then use distilled water to carry out cleaning 2 times ~ 3 times, at room temperature dry again, and then use transparent nail polish to be fixed on microslide by schizothoracin class otolith concave surface, then microslide is put into the dry 22h ~ 24h of baking oven that temperature is 33 DEG C ~ 35 DEG C.
9. the method for making of a kind of schizothoracin class otolith section according to claim 1, it is characterized in that using polishing paper to carry out polishing to the schizothoracin class otolith concave surface after polishing during step 5 2., and then use distilled water to carry out cleaning 2 times ~ 3 times, at room temperature dry again, and then use transparent nail polish to be fixed on microslide by schizothoracin class otolith concave surface, then microslide is put into the dry 20h ~ 22h of baking oven that temperature is 30 DEG C ~ 32 DEG C.
10. the method for making of a kind of schizothoracin class otolith section according to claim 1, it is characterized in that hand-held microslide during step 5 3., 500# ~ 600# sand paper and 1000# ~ 1200# sand paper is used to polish to that face hardening resin being embedded with schizothoracin class otolith convex surface of not polishing successively, often examine under a microscope its polishing degree after polishing 5s, until observe schizothoracin class otolith wheel line clearly, stop polishing, obtain the schizothoracin class otolith abrasive disc that thickness is 0.4mm ~ 0.5mm.
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