CN106813968A - A kind of preparation method of hairtail sagitta cross section - Google Patents
A kind of preparation method of hairtail sagitta cross section Download PDFInfo
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- CN106813968A CN106813968A CN201710072041.5A CN201710072041A CN106813968A CN 106813968 A CN106813968 A CN 106813968A CN 201710072041 A CN201710072041 A CN 201710072041A CN 106813968 A CN106813968 A CN 106813968A
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- China
- Prior art keywords
- otolith
- polishing
- pur
- hairtail
- sagitta
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/32—Polishing; Etching
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Dental Prosthetics (AREA)
Abstract
A kind of preparation method of hairtail sagitta cross section, the invention belongs to experimental technique field, it is comprised the following steps：1) mark otolith center；2) otolith is clamped on thin head self-locking forceps；3) otolith is embedded with PUR；4) polishing otolith is to mark line；5) buffed surface of otolith after polishing is close to and is bonded on slide；6) polished since otolith front end, be polishing to stop when otolith center, annual ring occur, polishing plane is all the time perpendicular to the cephalocaudal axis of otolith.The present invention is embedded with PUR to otolith, is served resin embedding and is fixed while with protective effect, and only several minutes can be cooled to the degree that can be polished；While saving resin embedding material, operating efficiency is improve.
Description
Technical field
The invention belongs to experimental technique field, more particularly to a kind of preparation method of hairtail sagitta cross section.
Background technology
When carrying out hairtail Age Determination, (hairtail 3 is as shown below to 1 couple maximum in otolith for its sagitta；Hereinafter
Abbreviation otolith) it is most commonly used material.Band fish otolith shape such as rice grain, small and crisp fritter；The band fish otolith of 500 grams of body weight, its
Length and width is also only 5-6mm and 2.5mm or so, the smaller person of the leisure opinion bodily form.Therefore in conventional method, prepare otolith cross section
When, multiplex epoxy resin is embedded, to carry out follow-up operation.It has main steps that：1) mark otolith center 2) use tree
Fat carries out embedding 3) after after the solidification of resin embedding block, cutting 4 is carried out with otolith cutting machine) use different grain size on otolith polishing and burnishing machine
Sand paper grinding and buffing is carried out to the resin embedding block after cutting, so as to obtain required otolith cross section.
Because the hardening time of resin embedding block is usually more than ten to twenties hours, required time is more long, therefore sternly
Important place have impact on operating efficiency.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of preparation method of hairtail sagitta cross section, methods described
Embedding speed is fast, can obtain band fish otolith cross section without cutting.
The present invention is achieved by the following technical solution：
A kind of preparation method of hairtail sagitta cross section, comprises the following steps：
1) mark otolith center；
2) otolith is clamped on thin head self-locking forceps；
3) otolith is embedded with PUR；
Further, the specific method of the embedding is heating PUR to 180 degrees Celsius；Otolith is immersed into hot melt adhesive liquid
In, otolith base leaf fore-end is reserved, PUR should not be adhered to, allow the other parts of otolith uniformly to be embedded by PUR.
4) polishing otolith is to mark line；
Further, described polishing process is to be polished since otolith rear end, and polishing plane is all the time perpendicular to the head of otolith
Tailing axle.
5) buffed surface of otolith after polishing is close to and is bonded on slide；
6) polished since otolith front end, be polishing to stop when otolith center, annual ring occur, beaten
Flat surface grinding is all the time perpendicular to the cephalocaudal axis of otolith.
Present invention beneficial effect compared with prior art
The present invention does not use resin embedding otolith, uses PUR instead and otolith is embedded, and serves resin embedding and fixes
While with protective effect, because PUR cooling velocity is fast, only several minutes can be cooled to the degree that can be polished；So
Time needed for substantially eliminating resin solidification.Meanwhile, directly polished using polishing and burnishing machine otolith and with cutting machine cut after again
In contrast, required time is suitable for the mode of polishing.Therefore while resin embedding material is saved, preferably improve work
Make efficiency.
Brief description of the drawings
Fig. 1 hairtail sagitta structural representations：1- winged petioles, 2- auditory nerve ditches, 3- front ends, 4- base leaves, 5- cephalocaudal axises, 6-
Ventral margin, 7- rear ends, 8- otoliths center, 9- back edges, 10- dorsoventral axis；
Fig. 2 mark otoliths center；
Be clamped in otolith on thin head self-locking forceps by Fig. 3；
Fig. 4 are embedded with PUR to otolith；
Fig. 5 polish otolith for the first time；
Be bonded in otolith on slide by Fig. 6；
Fig. 7 are polishing to otolith center, annual ring to be occurred.
Specific embodiment
Embodiment
Technical scheme is further explained below by embodiment combination accompanying drawing, but protection of the invention
Scope is not limited by embodiment is any in form.
1) cross otolith back edge peak with pencil and draw a line perpendicular to its cephalocaudal axis about 0.6mm wide with otolith center
(Fig. 2).
2) otolith after mark is clamped on thin head self-locking forceps, tweezer point is placed on otolith in auditory nerve ditch, after otolith
It is outer (Fig. 3) that end (part that needs grind off) is placed in tweezer point.
3) using the PUR (CrystalbondTM) in thermal station heating container to 180 degrees Celsius；Otolith is immersed and is heated
Glue, reserves a little part in otolith base leaf front end, should not adhere to PUR and (be easy to for the otolith after polishing first to be bonded in load glass
On piece), allow the other parts of otolith uniformly to be embedded (Fig. 4) by PUR.
4) otolith after embedding is placed on experimental bench；After after PUR cooling, hand-held tweezers are used on otolith polishing and burnishing machine
240 grit papers are polished；When polishing is nearly to mark line, uses 400 and 600 grit papers instead successively and be polishing to mark line
(Fig. 5).
5) slide is placed in thermal station, temperature is adjusted to 160 degree or so.One hand-held self-locking forceps, by the otolith after polishing
It is placed on slide, buffed surface is close to slide；One hand-held thin head tweezers, after the hotmelt melts of otolith to be wrapped, open certainly
Lock tweezers, the non-viscose glue part in otolith base leaf front end is clamped with thin head tweezers, is placed on slide (Fig. 6).
6) slide is removed from thermal station, after after PUR cooling, slide is put on slide fixture.Successively with 240,
The sand paper of 400 and 600 granularities is polished from otolith front end, and period is inspected with anatomical lens, is milled to otolith center, annual ring clear
Stop (Fig. 7) during appearance.
Claims (3)
1. a kind of preparation method of hairtail sagitta cross section, it is characterised in that it is comprised the following steps：
1) mark otolith center；
2) otolith is clamped on thin head self-locking forceps；
3) otolith is embedded with PUR；
4) polishing otolith is to mark line；
5) buffed surface of otolith after polishing is close to and is bonded on slide；
6) polished since otolith front end, be polishing to stop when otolith center, annual ring occur, polishing plane is all the time perpendicular to otolith
Cephalocaudal axis.
2. the preparation method of a kind of hairtail sagitta cross section according to claim 1, it is characterised in that the step 3)
The specific method of embedding is heating PUR to 180 degrees Celsius；Otolith is immersed in hot melt adhesive liquid, otolith base leaf leading section is reserved
Point, PUR should not be adhered to, allow the other parts of otolith uniformly to be embedded by PUR.
3. the preparation method of a kind of hairtail sagitta cross section according to claim 1, it is characterised in that the step 4)
Polishing process be since otolith rear end polish, polishing plane all the time perpendicular to the cephalocaudal axis of otolith.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710072041.5A CN106813968B (en) | 2017-02-09 | 2017-02-09 | A kind of preparation method of hairtail sagitta cross section |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710072041.5A CN106813968B (en) | 2017-02-09 | 2017-02-09 | A kind of preparation method of hairtail sagitta cross section |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106813968A true CN106813968A (en) | 2017-06-09 |
| CN106813968B CN106813968B (en) | 2018-10-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710072041.5A Expired - Fee Related CN106813968B (en) | 2017-02-09 | 2017-02-09 | A kind of preparation method of hairtail sagitta cross section |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU935742A1 (en) * | 1980-06-23 | 1982-06-15 | Московский областной научно-исследовательский институт акушерства и гинекологии | Method of localizing inflammatory process in placenta |
| CN101539495A (en) * | 2009-03-04 | 2009-09-23 | 上海海洋大学 | Preparation of mackerel otolith cross-sectional slices and method for determining age thereof |
| CN101548653A (en) * | 2009-05-12 | 2009-10-07 | 中国科学院水生生物研究所 | Method for researching age and migration life history of coilia by eardust |
| CN103231285A (en) * | 2013-04-26 | 2013-08-07 | 中国科学院水生生物研究所 | Manufacturing method and applications of otolith cross section grinding sheet of mandarin fish |
| CN103518655A (en) * | 2013-10-14 | 2014-01-22 | 中国水产科学研究院长江水产研究所 | Method for manufacturing section of xenophysogobio boulengeri otolith |
| CN104374615A (en) * | 2014-09-16 | 2015-02-25 | 中国水产科学研究院黑龙江水产研究所 | Schizothoracinae otolith slice production method |
| CN105136541A (en) * | 2015-09-21 | 2015-12-09 | 中国水产科学研究院淡水渔业研究中心 | Pretreatment method for micro-chemical analysis of otoliths of Coilia nasus |
-
2017
- 2017-02-09 CN CN201710072041.5A patent/CN106813968B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU935742A1 (en) * | 1980-06-23 | 1982-06-15 | Московский областной научно-исследовательский институт акушерства и гинекологии | Method of localizing inflammatory process in placenta |
| CN101539495A (en) * | 2009-03-04 | 2009-09-23 | 上海海洋大学 | Preparation of mackerel otolith cross-sectional slices and method for determining age thereof |
| CN101548653A (en) * | 2009-05-12 | 2009-10-07 | 中国科学院水生生物研究所 | Method for researching age and migration life history of coilia by eardust |
| CN103231285A (en) * | 2013-04-26 | 2013-08-07 | 中国科学院水生生物研究所 | Manufacturing method and applications of otolith cross section grinding sheet of mandarin fish |
| CN103518655A (en) * | 2013-10-14 | 2014-01-22 | 中国水产科学研究院长江水产研究所 | Method for manufacturing section of xenophysogobio boulengeri otolith |
| CN104374615A (en) * | 2014-09-16 | 2015-02-25 | 中国水产科学研究院黑龙江水产研究所 | Schizothoracinae otolith slice production method |
| CN105136541A (en) * | 2015-09-21 | 2015-12-09 | 中国水产科学研究院淡水渔业研究中心 | Pretreatment method for micro-chemical analysis of otoliths of Coilia nasus |
Non-Patent Citations (2)
| Title |
|---|
| 宋昭彬 等: "人工饲养和野生草鱼幼鱼耳石微结构的比较研究", 《水生生物学报》 * |
| 罗燕平: "广州从化拟细鲫基础生物学的研究", 《华南师范大学2010学位年度硕士学位论文》 * |
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|---|---|
| CN106813968B (en) | 2018-10-12 |
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Granted publication date: 20181012 Termination date: 20210209 |