CN103518655A - Method for manufacturing section of xenophysogobio boulengeri otolith - Google Patents
Method for manufacturing section of xenophysogobio boulengeri otolith Download PDFInfo
- Publication number
- CN103518655A CN103518655A CN201310477811.6A CN201310477811A CN103518655A CN 103518655 A CN103518655 A CN 103518655A CN 201310477811 A CN201310477811 A CN 201310477811A CN 103518655 A CN103518655 A CN 103518655A
- Authority
- CN
- China
- Prior art keywords
- otolith
- baking
- neutral gum
- drip
- tuo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for manufacturing a section of a xenophysogobio boulengeri otolith for fish age determination. The otolith of xenophysogobio boulengeri is picked, soaked, fixed, baked, cooled, grinded, degummed and mounted, clear annulations appear on the otolith of the xenophysogobio boulengeri, and therefore the age of the xenophysogobio boulengeri is determined. The new method is provided for fish age study, and is suitable for the study of the age of teleostean with two sides of the otoliths thick.
Description
Technical field
The present invention relates to the evaluation at fish age.
Background technology
Different Biao Qiu Tuo is under the jurisdiction of the different Biao Qiu of Cypriniformes Cyprinidae Qiu Tuo subfamily Tuo and belongs to, and is Upper Yangtze River Endemic fish, is mainly distributed in Upper Yangtze River master stream and tributary thereof, conventionally inhabit at the bottom of the sandstone at rivers flowing water place on, take benthonic invertebrates as food.Due to the foundation of Yangtze River Gorges dam, there is larger variation in Upper Yangtze River ecotope, and different these distinctive fish of Biao Qiu Tuo reduce gradually.In order to protect this distinctive fish, therefore different this Endemic fish of Biao Qiu Tuo to be carried out to age research and be necessary, this resource management and protection to different Biao Qiu Tuo has great importance.
At present, the common used material of fish Age Determination is had to scale, otolith, fin ray, nucleus, vertebrae etc., wherein the most frequently used is scale and otolith.Conventionally to the different Biao Qiu Tuo age, adopt scale to identify, and the outside scale of different Biao Qiu Tuo container and be often subject to washing away of current very, scale easily comes off and forms secondary squama, and it is damaged or cracked that the wheel line that its scale is made has, and affects the interpretation of annual ring.Therefore with scale, identify that the age data of different Biao Qiu Tuo is inaccurate.
Summary of the invention
The object of the invention is to overcome above-mentioned technical deficiency, a kind of microsection manufacture method of different Biao Qiu Tuo otolith has been proposed, it is that the i-coch otolith in different Biao Qiu Tuo is taken out by microsection manufacture and becomes otolith wheel line to manifest obvious method, thereby judges the age of different Biao Qiu Tuo.
Technical scheme of the present invention is as follows:
Because otolith is a kind of calcium carbonate sclerous tissues being positioned in fish inner ear, mainly that calcium carbonate forms, not only show a year deposition, and still can continued growth when the malnutritive environmental conditions such as hunger, even be difficult for while being subject to environmental pressure, be absorbed, once and form and just to remain unchanged, thereby become and identify the most accurate material of fish age.
Preparation method of the present invention is: 1, win, 2, soak, 3, fixing, 4, baking, 5, coolly put, 6, abrasive disc, 7, except glue, 8, mounting:
1, win: with scissors and tweezers, the otolith in different Biao Qiu Tuo cochlea inner equilibrium capsule is taken out, and pick the impurity such as clean cochlea.
2, soak: after otolith is taken out, put into 2-10mlEP pipe, adding concentration is more than 95% alcohol-pickled otolith, and otolith is immersed in to concentration is 3-5 days in more than 95% alcohol;
3, fixing: the otolith after soaking to be taken out and put on slide, drip a 1-2 and drip neutral gum and cover the i.e. fixing otolith of otolith;
4, baking: the fixing slide of otolith is put into baking oven and toast, the temperature of baking is 33-38 ℃, and baking time is 20-30 hour, and the temperature of its baking is higher, and the time of baking is shorter, and the temperature of baking is lower, and the time of baking is long;
5, cool putting: after the otolith baking on slide, take out and be placed on ventilation 5-7 hour, make it cooling and dry.
6, abrasive disc: with the sand paper of No. 3000-5000 polishing that gets wet, limit polishing, limit is observed, and is milled to behind otolith center, with alcolhol burner, neutral gum is melted, after neutral gum fusing, with needle point, the another side of otolith is routed up, then drip 1-2 and drip neutral gum and cover otolith, and then be baked to after neutral gum fusing with alcolhol burner, slide placement is cooling to neutral gum on one side, then wash the impurity being stained with while toasting, and then repeat polishing until otolith center.
7, except glue: drip 1-2 melted paraxylene on otolith after polishing, wait after neutral gum dissolves in dimethylbenzene, on the side of otolith, paper using slowly blots dimethylbenzene, and otolith just can manifest wheel line clearly, can judge, identify the age of different Biao Qiu Tuo;
8, mounting: otolith is after processing except glue, and a 1-2 drips neutral gum and covers otolith, gets final product long preservation.
Good effect of the present invention is:
1, because different Biao Qiu Tuo otolith is very little, two sides is thicker, and wheel line is lighter, the present invention is by the otolith microsection manufacture to different Biao Qiu Tuo, after the polishing of turn-over two sides, the scale wheel line of the different Biao Qiu of the wheel line aspect ratio Tuo on its otolith abrasive disc is more directly perceived and clear, and its accuracy is higher;
2, the present invention is for fish age research provides a kind of new method, filled up all blank of bright band and the unsharp age research of blanking bar on thicker otolith of two sides, and has been applicable to the research at thicker bony fish age of all otoliths two sides.
Embodiment
By the following examples 1,2, the invention will be further described.
Embodiment mono-
1. win: after with scissors and tweezers, the otolith in the statocyst in different Biao Qiu Tuo cochlea being taken out, pick the impurity such as clean cochlea;
2. soak: the otolith that picks the impurity such as clean cochlea is put into 5mlEP pipe, and adding concentration is 95% alcohol otolith is immersed in to concentration is to soak in 95% alcohol 5 days;
3. fixing: the otolith after soaking to be taken out and puts into slide and dry, and drip 1 neutral gum and cover otolith and fix;
4. baking: the slide of the otolith after fixing is put into baking oven and toasted, and the temperature of baking is 33 ℃, and the time of baking is 30 hours;
5. cool putting: the otolith on slide, after baking, takes out and is placed on ventilation 7 hours, cooling drying;
6. abrasive disc: with the sand paper of No. 3000 along the water otolith of polishing, limit polishing, limit is observed, and is milled to behind otolith center, with alcolhol burner, neutral gum is melted, then with needle point, the another side of otolith is rolled up, drip again 1 neutral gum and cover after otolith, with alcolhol burner, be baked to after neutral gum fusing, Yi Bian it is cooling that slide placement is cooled to neutral gum, wash again the impurity that baking is stained with, and then repeat polishing until otolith center;
7. except glue: dimethylbenzene is dripped to 2 and drops on otolith, neutral gum is dissolved, then otolith side paper using slowly blot dimethylbenzene, now on otolith, show and take turns clearly line;
8. mounting: drip 2 neutral gums on the otolith except after glue, and get final product long preservation after covering otolith.
Embodiment bis-
1. win: after with scissors and tweezers, the otolith in the statocyst in different Biao Qiu Tuo cochlea being taken out, pick the impurity such as clean cochlea;
2. soak: the otolith that picks the impurity such as clean cochlea is put into 10mlEP pipe, and adding concentration is 96% alcohol otolith is immersed in to concentration is to soak in 96% alcohol 4 days;
3. fixing: the otolith taking-up after soaking is put into slide and dry, and 2 neutral gums covering otoliths to be fixed;
4. baking: the slide of the otolith after fixing is put into baking oven and toasted, and the temperature of baking is 35 ℃, and the time of baking is 25 hours;
5. cool putting: the otolith on slide, after baking, takes out and is placed on ventilation 6 hours, cooling drying;
6. abrasive disc: with the sand paper of No. 5000 along the water otolith of polishing, limit polishing, limit is observed, be milled to behind otolith center, with alcolhol burner, neutral gum melted, then with needle point, the another side of otolith is rolled up, drip again after 2 neutral gums cover otolith and be baked to after neutral gum fusing with alcolhol burner, slide placement is cooled to neutral gum on one side cooling, then washes the impurity that baking is stained with, and then repeat polishing until otolith center;
7. except glue: dimethylbenzene is dripped to 2 and drops on otolith, neutral gum is dissolved, then otolith side paper using slowly blot dimethylbenzene, now on otolith, show and take turns clearly line;
8. mounting: drip 2 neutral gums on the otolith except after glue, and get final product long preservation after covering otolith.
Embodiment tri-
1. win: after with scissors and tweezers, the otolith in the statocyst in different Biao Qiu Tuo cochlea being taken out, pick the impurity such as clean cochlea;
2. soak: the otolith that picks the impurity such as clean cochlea is put into 5mlEP pipe, and adding concentration is 98% alcohol otolith is immersed in to concentration is to soak in 98% alcohol 3 days;
3. fixing: the otolith taking-up after soaking is put into slide and dry, and 2 neutral gums covering otoliths to be fixed;
4. baking: the slide of the otolith after fixing is put into baking oven and toasted, and the temperature of baking is 38 ℃, and the time of baking is 20 hours;
5. cool putting: the otolith on slide, after baking, takes out and is placed on ventilation 5 hours, cooling drying;
6. abrasive disc: with the sand paper of No. 3000 along the water otolith of polishing, limit polishing, limit is observed, be milled to behind otolith center, with alcolhol burner, neutral gum melted, then with needle point, the another side of otolith is rolled up, drip again after 2 neutral gums cover otolith and be baked to after neutral gum fusing with alcolhol burner, slide placement is cooled to neutral gum on one side cooling, then washes the impurity that baking is stained with, and then repeat polishing until otolith center;
7. except glue: dimethylbenzene is dripped to 2 and drops on otolith, neutral gum is dissolved, then otolith side paper using slowly blot dimethylbenzene, now on otolith, show and take turns clearly line;
8. mounting: drip 2 neutral gums on the otolith except after glue, and get final product long preservation after covering otolith.
Claims (1)
1. a microsection manufacture method for different fish glue loach Tuo otolith, is characterized in that: 1, win, 2, soak, 3, fixing, 4, baking, 5, coolly put, 6, abrasive disc, 7, except glue, 8, mounting:
1), win: with scissors and tweezers, the otolith in different Biao Qiu Tuo cochlea inner equilibrium capsule is taken out, and pick the impurity such as clean ear;
?2), soak: after otolith is taken out, put into 2-10mlEP pipe, adding concentration is the immersion otolith of 95% above alcohol, and otolith is immersed in to the middle 3-5 days that concentration is 95% above alcohol;
3), fixing: the otolith after soaking to be taken out and put on slide, drip 1-2 and drip neutral gum and cover otolith;
4), baking: the fixing slide of otolith is put into baking oven and toast, the temperature of baking is 33-38 ℃, and baking time is 20-30 hour, and the temperature of its baking is higher, and the time of baking is shorter, and the temperature of baking is lower, and the time of baking is long;
5), cool putting: after the otolith baking on slide, take out and be placed on ventilation 5-7 hour, make it cooling and dry;
6), abrasive disc: with the sand paper of No. 3000-5000 polishing that gets wet, limit polishing, limit is observed, and is milled to behind otolith center, with alcolhol burner, neutral gum is melted, after neutral gum fusing, with needle point, the another side of otolith is routed up, then drip 1-2 and drip neutral gum and cover otolith, and then be baked to after neutral gum fusing with alcolhol burner, slide placement is cooling to neutral gum on one side, then wash the impurity being stained with while toasting, and then repeat polishing until otolith center;
7), except glue: drip 1-2 melted paraxylene on otolith after polishing, Deng neutral gum, dissolve after dimethylbenzene, on the side of otolith, paper using slowly blots dimethylbenzene, except the otolith after glue just can manifest wheel line clearly, can judge, identify the age of different Biao Qiu Tuo;
8), mounting: otolith is after processing except glue, and a 1-2 drips neutral gum and covers otolith, gets final product long preservation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310477811.6A CN103518655A (en) | 2013-10-14 | 2013-10-14 | Method for manufacturing section of xenophysogobio boulengeri otolith |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310477811.6A CN103518655A (en) | 2013-10-14 | 2013-10-14 | Method for manufacturing section of xenophysogobio boulengeri otolith |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103518655A true CN103518655A (en) | 2014-01-22 |
Family
ID=49921297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310477811.6A Pending CN103518655A (en) | 2013-10-14 | 2013-10-14 | Method for manufacturing section of xenophysogobio boulengeri otolith |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103518655A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103843708A (en) * | 2014-03-17 | 2014-06-11 | 上海海洋大学 | Method for constructing saury otolith physiological state analyzing model |
| CN104374615A (en) * | 2014-09-16 | 2015-02-25 | 中国水产科学研究院黑龙江水产研究所 | Schizothoracinae otolith slice production method |
| CN105738175A (en) * | 2016-03-04 | 2016-07-06 | 中国水产科学研究院北戴河中心实验站 | Method for preparing otolith sample for early life history of fish |
| CN106813968A (en) * | 2017-02-09 | 2017-06-09 | 中国水产科学研究院黄海水产研究所 | A kind of preparation method of hairtail sagitta cross section |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101539495A (en) * | 2009-03-04 | 2009-09-23 | 上海海洋大学 | Preparation of mackerel otolith cross-sectional slices and method for determining age thereof |
| CN101548653A (en) * | 2009-05-12 | 2009-10-07 | 中国科学院水生生物研究所 | Method for researching age and migration life history of coilia by eardust |
| CN102172882A (en) * | 2011-01-28 | 2011-09-07 | 上海海洋大学 | Method for grinding and preserving cephalopod otolith slices |
| CN103231285A (en) * | 2013-04-26 | 2013-08-07 | 中国科学院水生生物研究所 | Manufacturing method and applications of otolith cross section grinding sheet of mandarin fish |
-
2013
- 2013-10-14 CN CN201310477811.6A patent/CN103518655A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101539495A (en) * | 2009-03-04 | 2009-09-23 | 上海海洋大学 | Preparation of mackerel otolith cross-sectional slices and method for determining age thereof |
| CN101548653A (en) * | 2009-05-12 | 2009-10-07 | 中国科学院水生生物研究所 | Method for researching age and migration life history of coilia by eardust |
| CN102172882A (en) * | 2011-01-28 | 2011-09-07 | 上海海洋大学 | Method for grinding and preserving cephalopod otolith slices |
| CN103231285A (en) * | 2013-04-26 | 2013-08-07 | 中国科学院水生生物研究所 | Manufacturing method and applications of otolith cross section grinding sheet of mandarin fish |
Non-Patent Citations (2)
| Title |
|---|
| 王生: "异鳔鳅鮀（Xenophysogobio boulengeri）生物学及资源量初步研究", 《中国优秀硕士学位论文全文数据库》, no. 9, 15 September 2012 (2012-09-15), pages 052 - 1 * |
| 王生等: "异鳔鳅鮀年龄结构、生长特性与生活史类型", 《动物学杂志》, vol. 47, no. 3, 20 June 2012 (2012-06-20), pages 1 - 8 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103843708A (en) * | 2014-03-17 | 2014-06-11 | 上海海洋大学 | Method for constructing saury otolith physiological state analyzing model |
| CN104374615A (en) * | 2014-09-16 | 2015-02-25 | 中国水产科学研究院黑龙江水产研究所 | Schizothoracinae otolith slice production method |
| CN105738175A (en) * | 2016-03-04 | 2016-07-06 | 中国水产科学研究院北戴河中心实验站 | Method for preparing otolith sample for early life history of fish |
| CN105738175B (en) * | 2016-03-04 | 2018-07-31 | 中国水产科学研究院北戴河中心实验站 | A kind of fish early life history otolith sample preparation methods |
| CN106813968A (en) * | 2017-02-09 | 2017-06-09 | 中国水产科学研究院黄海水产研究所 | A kind of preparation method of hairtail sagitta cross section |
| CN106813968B (en) * | 2017-02-09 | 2018-10-12 | 中国水产科学研究院黄海水产研究所 | A kind of preparation method of hairtail sagitta cross section |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103518655A (en) | Method for manufacturing section of xenophysogobio boulengeri otolith | |
| CN104983039A (en) | Method for fixation and softening of areca nuts | |
| CN102144786A (en) | Method for preparing instant smoked fish food | |
| CN103907914A (en) | Novel method for preparing canned mushroom | |
| CN108308076A (en) | A kind of Chlamys nobilis " Nan'ao gold shellfish " new culturing technique | |
| CN104365557A (en) | Artificial rearing, breed conservation and breeding production method for cryptolaemus montrouzieri mulsant | |
| CN103564332A (en) | Preparation process for wet rice vermicelli with long shelf life | |
| CN105285075A (en) | Peach preservative and production method thereof | |
| CN103548696A (en) | Method for quickly and efficiently breeding blueberry seedlings | |
| Sun et al. | Bottom-up control of phytoplankton growth in spring blooms in Central Yellow Sea, China | |
| CN103231285B (en) | A kind of preparation method of mandarin fish otolith cross section abrasive disc and application thereof | |
| CN103348801A (en) | Method for enhancing mitsuba seed germination rate | |
| Huq | Studies on the epidemiology of leaf rot and leaf spot diseases of betel vine (Piper betle L.) | |
| CN105738175B (en) | A kind of fish early life history otolith sample preparation methods | |
| CN103766563A (en) | Preparation method for candied melon | |
| CN103783182A (en) | Black tea fermentation process | |
| CN107379181A (en) | A kind of willow furniture waxing method | |
| KR20180073252A (en) | Method for manufacturing of laver and the laver manufactured thereby | |
| Breeman et al. | Ecology and distribution of the subtidal red alga Acrosymphyton purpuriferum (J. Ag.) Sjöst.(Rhodophyceae, Cryptonemiales) | |
| CN103270992B (en) | Rose-red color pearl growing method | |
| CN106107834A (en) | A kind of fried green fruit kernel and preparation method thereof | |
| Koelkebeck | What is egg shell quality and how to preserve it | |
| CN101946678A (en) | Method for cultivating sedum shitaiense Yan Zheng et D.C.Zhang | |
| CN101317654A (en) | Chuanminshen viloaceum Sheh et Shan processing method | |
| CN103766853A (en) | Soybean paste preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C05 | Deemed withdrawal (patent law before 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140122 |